AlfalfaGEDB | MS.gene42623

M. sativa cultivar XinJiangDaYe / MS.gene42623

KEGG: -
GO: GO:0005575 GO:0008150 GO:0008152 GO:0009521 GO:0009523 GO:0009987 GO:0015979 GO:0016020 GO:0032991 GO:0044237 GO:0098796 GO:0110165
PFAM: PF06596.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42623.t1	RHN70500.1	96.7	120	4	0	1	120	24	143	1.40E-50	208.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42623.t1	G7J8E2	96.7	120	4	0	1	120	1	120	1.0e-50	208.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049173	MS.gene42623	0.814898	1.22E-51	-1.69E-46
MS.gene049285	MS.gene42623	0.802693	4.97E-49	-1.69E-46
MS.gene049320	MS.gene42623	0.803417	3.52E-49	-1.69E-46
MS.gene049609	MS.gene42623	0.81153	6.69E-51	-1.69E-46
MS.gene049614	MS.gene42623	0.802443	5.60E-49	-1.69E-46
MS.gene050767	MS.gene42623	0.848875	4.64E-60	-1.69E-46
MS.gene050770	MS.gene42623	0.850608	1.52E-60	-1.69E-46
MS.gene050772	MS.gene42623	0.84661	1.95E-59	-1.69E-46
MS.gene05100	MS.gene42623	0.808663	2.77E-50	-1.69E-46
MS.gene05105	MS.gene42623	0.807233	5.59E-50	-1.69E-46
MS.gene051228	MS.gene42623	0.811093	8.32E-51	-1.69E-46
MS.gene051666	MS.gene42623	0.801135	1.04E-48	-1.69E-46
MS.gene051692	MS.gene42623	0.826535	2.57E-54	-1.69E-46
MS.gene051858	MS.gene42623	0.827765	1.31E-54	-1.69E-46
MS.gene052271	MS.gene42623	0.843303	1.52E-58	-1.69E-46
MS.gene052421	MS.gene42623	0.815855	7.47E-52	-1.69E-46
MS.gene053009	MS.gene42623	0.817999	2.46E-52	-1.69E-46
MS.gene053103	MS.gene42623	0.805936	1.05E-49	-1.69E-46
MS.gene053207	MS.gene42623	0.806216	9.16E-50	-1.69E-46
MS.gene053209	MS.gene42623	0.811402	7.13E-51	-1.69E-46
MS.gene053213	MS.gene42623	0.815344	9.70E-52	-1.69E-46
MS.gene053286	MS.gene42623	0.800423	1.45E-48	-1.69E-46
MS.gene053322	MS.gene42623	0.820302	7.37E-53	-1.69E-46
MS.gene053449	MS.gene42623	0.809052	2.29E-50	-1.69E-46
MS.gene053632	MS.gene42623	0.800781	1.23E-48	-1.69E-46
MS.gene053813	MS.gene42623	0.807921	3.99E-50	-1.69E-46
MS.gene053916	MS.gene42623	0.814156	1.78E-51	-1.69E-46
MS.gene054376	MS.gene42623	0.825703	4.06E-54	-1.69E-46
MS.gene054600	MS.gene42623	0.813718	2.22E-51	-1.69E-46
MS.gene054642	MS.gene42623	0.802731	4.88E-49	-1.69E-46
MS.gene054802	MS.gene42623	0.809009	2.34E-50	-1.69E-46
MS.gene054803	MS.gene42623	0.803741	3.02E-49	-1.69E-46
MS.gene054804	MS.gene42623	0.810904	9.14E-51	-1.69E-46
MS.gene05505	MS.gene42623	0.824597	7.41E-54	-1.69E-46
MS.gene055212	MS.gene42623	0.817349	3.45E-52	-1.69E-46
MS.gene055254	MS.gene42623	0.835011	2.15E-56	-1.69E-46
MS.gene055362	MS.gene42623	0.828038	1.12E-54	-1.69E-46
MS.gene055499	MS.gene42623	0.824522	7.71E-54	-1.69E-46
MS.gene055722	MS.gene42623	0.860848	1.55E-63	-1.69E-46
MS.gene05592	MS.gene42623	0.834473	2.94E-56	-1.69E-46
MS.gene056191	MS.gene42623	0.805158	1.53E-49	-1.69E-46
MS.gene056374	MS.gene42623	0.827906	1.21E-54	-1.69E-46
MS.gene056410	MS.gene42623	0.830639	2.62E-55	-1.69E-46
MS.gene056611	MS.gene42623	0.805934	1.05E-49	-1.69E-46
MS.gene056657	MS.gene42623	0.800128	1.67E-48	-1.69E-46
MS.gene057129	MS.gene42623	0.846798	1.73E-59	-1.69E-46
MS.gene057295	MS.gene42623	0.809734	1.63E-50	-1.69E-46
MS.gene057331	MS.gene42623	0.825312	5.02E-54	-1.69E-46
MS.gene057416	MS.gene42623	0.840617	7.80E-58	-1.69E-46
MS.gene057926	MS.gene42623	0.815715	8.02E-52	-1.69E-46
MS.gene058006	MS.gene42623	0.801429	9.04E-49	-1.69E-46
MS.gene058293	MS.gene42623	0.822432	2.38E-53	-1.69E-46
MS.gene058323	MS.gene42623	0.826543	2.56E-54	-1.69E-46
MS.gene058419	MS.gene42623	0.810033	1.41E-50	-1.69E-46
MS.gene058723	MS.gene42623	0.823302	1.49E-53	-1.69E-46
MS.gene058773	MS.gene42623	0.81225	4.66E-51	-1.69E-46
MS.gene059022	MS.gene42623	0.814147	1.79E-51	-1.69E-46
MS.gene059536	MS.gene42623	0.811039	8.55E-51	-1.69E-46
MS.gene059711	MS.gene42623	0.804644	1.96E-49	-1.69E-46
MS.gene059718	MS.gene42623	0.807397	5.16E-50	-1.69E-46
MS.gene059902	MS.gene42623	0.827494	1.52E-54	-1.69E-46
MS.gene059960	MS.gene42623	0.806045	9.95E-50	-1.69E-46
MS.gene059974	MS.gene42623	0.810946	8.95E-51	-1.69E-46
MS.gene060193	MS.gene42623	0.802305	5.97E-49	-1.69E-46
MS.gene060804	MS.gene42623	0.800814	1.21E-48	-1.69E-46
MS.gene060837	MS.gene42623	0.815434	9.26E-52	-1.69E-46
MS.gene061041	MS.gene42623	0.801898	7.25E-49	-1.69E-46
MS.gene061200	MS.gene42623	0.846818	1.71E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42623.t1	MTR_3g104950	96.667	120	4	0	1	120	1	120	1.26e-78	227
MS.gene42623.t1	MTR_1g015290	66.129	124	37	2	1	119	1	124	5.57e-48	150
MS.gene42623.t1	MTR_4g125600	57.851	121	44	5	1	119	1	116	5.01e-35	117
MS.gene42623.t1	MTR_2g041260	55.738	122	48	3	1	119	1	119	2.54e-24	90.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42623.t1	AT2G06520	52.427	103	46	1	20	119	13	115	2.39e-24	90.1

Find 22 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTATACTTGGAGCTGTTAT+TGG	0.192002	3.4:+91892185	MS.gene42623:CDS
TTTGACTGTGTGATCCTCTT+TGG	0.259959	3.4:-91891987	None:intergenic
CTATACTTGGAGCTGTTATT+GGG	0.283981	3.4:+91892186	MS.gene42623:CDS
ATTAATTAAGCTCTCTTAAC+CGG	0.332183	3.4:-91892224	None:intergenic
TTTGGTTGTGCGATGGGTAA+TGG	0.371937	3.4:-91891969	None:intergenic
AGATGCATTAATTTCAACCT+TGG	0.419109	3.4:-91892012	None:intergenic
TAATGCATCTTTGAAAGAAA+AGG	0.433518	3.4:+91892025	MS.gene42623:CDS
CCTGATGTGGCTCATGCAGC+TGG	0.437171	3.4:+91892095	MS.gene42623:CDS
ATCCTCTTTGGTTGTGCGAT+GGG	0.483107	3.4:-91891975	None:intergenic
TGGGGTTTCCAACTTTGATC+CGG	0.483190	3.4:+91892205	MS.gene42623:CDS
CCAGCTGCATGAGCCACATC+AGG	0.505992	3.4:-91892095	None:intergenic
GATCCTCTTTGGTTGTGCGA+TGG	0.509446	3.4:-91891976	None:intergenic
CTCCATGGTTATACCTGATG+TGG	0.524546	3.4:+91892082	MS.gene42623:CDS
TCTCTTAACCGGATCAAAGT+TGG	0.532912	3.4:-91892213	None:intergenic
TGCATCCTTGACAGCCTCCA+TGG	0.539158	3.4:+91892067	MS.gene42623:CDS
GCTGTGCTAACAGCTATACT+TGG	0.540967	3.4:+91892173	MS.gene42623:CDS
TATACTTGGAGCTGTTATTG+GGG	0.562747	3.4:+91892187	MS.gene42623:CDS
TATAACCATGGAGGCTGTCA+AGG	0.564152	3.4:-91892072	None:intergenic
TACCCATCGCACAACCAAAG+AGG	0.604798	3.4:+91891973	MS.gene42623:CDS
GATCACACAGTCAAAATCCA+AGG	0.634917	3.4:+91891995	MS.gene42623:CDS
AGCCACATCAGGTATAACCA+TGG	0.670542	3.4:-91892084	None:intergenic
CACATCAGGTATAACCATGG+AGG	0.712255	3.4:-91892081	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATGCATCTTTGAAAGAAA+AGG	+	chr3.4:91892025-91892044	MS.gene42623:CDS	25.0%
	AAATGCTTAGCAAAAAGTTC+TGG	-	chr3.4:91892142-91892161	None:intergenic	30.0%
	AGATGCATTAATTTCAACCT+TGG	-	chr3.4:91892015-91892034	None:intergenic	30.0%
!	TTTTTGCTAAGCATTTTTGC+TGG	+	chr3.4:91892146-91892165	MS.gene42623:CDS	30.0%
!!	ATGGATTTTTCAGCTTGTTT+TGG	-	chr3.4:91891929-91891948	None:intergenic	30.0%
!!	TGGATTTTTCAGCTTGTTTT+GGG	-	chr3.4:91891928-91891947	None:intergenic	30.0%
	CTATACTTGGAGCTGTTATT+GGG	+	chr3.4:91892186-91892205	MS.gene42623:CDS	35.0%
	TAATGGCCTGAAAAATGACT+CGG	-	chr3.4:91891955-91891974	None:intergenic	35.0%
	TATACTTGGAGCTGTTATTG+GGG	+	chr3.4:91892187-91892206	MS.gene42623:CDS	35.0%
	GATCACACAGTCAAAATCCA+AGG	+	chr3.4:91891995-91892014	MS.gene42623:CDS	40.0%
	GCTATACTTGGAGCTGTTAT+TGG	+	chr3.4:91892185-91892204	MS.gene42623:CDS	40.0%
	TCTCTTAACCGGATCAAAGT+TGG	-	chr3.4:91892216-91892235	None:intergenic	40.0%
	TTTGACTGTGTGATCCTCTT+TGG	-	chr3.4:91891990-91892009	None:intergenic	40.0%
!	TTGCTAAGCATTTTTGCTGG+TGG	+	chr3.4:91892149-91892168	MS.gene42623:CDS	40.0%
!!	AGCAAAAAGTTCTGGAGAGA+TGG	-	chr3.4:91892134-91892153	None:intergenic	40.0%
	AGCCACATCAGGTATAACCA+TGG	-	chr3.4:91892087-91892106	None:intergenic	45.0%
	ATCCTCTTTGGTTGTGCGAT+GGG	-	chr3.4:91891978-91891997	None:intergenic	45.0%
	CACATCAGGTATAACCATGG+AGG	-	chr3.4:91892084-91892103	None:intergenic	45.0%
	CTCCATGGTTATACCTGATG+TGG	+	chr3.4:91892082-91892101	MS.gene42623:CDS	45.0%
	GCTGTGCTAACAGCTATACT+TGG	+	chr3.4:91892173-91892192	MS.gene42623:CDS	45.0%
	TATAACCATGGAGGCTGTCA+AGG	-	chr3.4:91892075-91892094	None:intergenic	45.0%
	TGGGGTTTCCAACTTTGATC+CGG	+	chr3.4:91892205-91892224	MS.gene42623:CDS	45.0%
	TTTGGTTGTGCGATGGGTAA+TGG	-	chr3.4:91891972-91891991	None:intergenic	45.0%
!	CTGAAAAATGACTCGGAGGA+TGG	-	chr3.4:91891948-91891967	None:intergenic	45.0%
!!	TTTTGGGTAGCAGAACTCAG+TGG	-	chr3.4:91891912-91891931	None:intergenic	45.0%
!!!	CATCCTCCGAGTCATTTTTC+AGG	+	chr3.4:91891946-91891965	MS.gene42623:CDS	45.0%
	GATCCTCTTTGGTTGTGCGA+TGG	-	chr3.4:91891979-91891998	None:intergenic	50.0%
	TACCCATCGCACAACCAAAG+AGG	+	chr3.4:91891973-91891992	MS.gene42623:CDS	50.0%
!	TGGCCTGAAAAATGACTCGG+AGG	-	chr3.4:91891952-91891971	None:intergenic	50.0%
	TGCATCCTTGACAGCCTCCA+TGG	+	chr3.4:91892067-91892086	MS.gene42623:CDS	55.0%
	CCAGCTGCATGAGCCACATC+AGG	-	chr3.4:91892098-91892117	None:intergenic	60.0%
	CCTGATGTGGCTCATGCAGC+TGG	+	chr3.4:91892095-91892114	MS.gene42623:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	91891879	91892241	91891879	ID=MS.gene42623
chr3.4	mRNA	91891879	91892241	91891879	ID=MS.gene42623.t1;Parent=MS.gene42623
chr3.4	exon	91891879	91892241	91891879	ID=MS.gene42623.t1.exon1;Parent=MS.gene42623.t1
chr3.4	CDS	91891879	91892241	91891879	ID=cds.MS.gene42623.t1;Parent=MS.gene42623.t1

Gene Sequence	>MS.gene42623 ATGGCTTCTGCTTCTGTTGCAACAGCTTTGCCACTGAGTTCTGCTACCCAAAACAAGCTGAAAAATCCATCCTCCGAGTCATTTTTCAGGCCATTACCCATCGCACAACCAAAGAGGATCACACAGTCAAAATCCAAGGTTGAAATTAATGCATCTTTGAAAGAAAAGGCAGTGACAGCACTTACAGCTGCATCCTTGACAGCCTCCATGGTTATACCTGATGTGGCTCATGCAGCTGGATCTGATCTCTCACCATCTCTCCAGAACTTTTTGCTAAGCATTTTTGCTGGTGGAGCTGTGCTAACAGCTATACTTGGAGCTGTTATTGGGGTTTCCAACTTTGATCCGGTTAAGAGAGCTTAA
Protein sequence	>MS.gene42623.t1 MASASVATALPLSSATQNKLKNPSSESFFRPLPIAQPKRITQSKSKVEINASLKEKAVTALTAASLTASMVIPDVAHAAGSDLSPSLQNFLLSIFAGGAVLTAILGAVIGVSNFDPVKRA