AlfalfaGEDB | MS.gene52607

M. sativa cultivar XinJiangDaYe / MS.gene52607

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52607.t1	XP_003624221.2	98.9	185	2	0	1	185	1	185	1.70E-98	368.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52607.t1	G7KSF0	98.9	185	2	0	1	185	1	185	1.2e-98	368.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049133	MS.gene52607	0.811361	7.27E-51	-1.69E-46
MS.gene049397	MS.gene52607	0.814923	1.20E-51	-1.69E-46
MS.gene049418	MS.gene52607	0.830543	2.77E-55	-1.69E-46
MS.gene049622	MS.gene52607	0.835468	1.65E-56	-1.69E-46
MS.gene049660	MS.gene52607	0.839857	1.23E-57	-1.69E-46
MS.gene049989	MS.gene52607	0.899089	2.80E-77	-1.69E-46
MS.gene050297	MS.gene52607	0.889422	2.46E-73	-1.69E-46
MS.gene050310	MS.gene52607	0.800801	1.22E-48	-1.69E-46
MS.gene05054	MS.gene52607	0.844993	5.36E-59	-1.69E-46
MS.gene050589	MS.gene52607	0.807629	4.60E-50	-1.69E-46
MS.gene050648	MS.gene52607	0.818068	2.38E-52	-1.69E-46
MS.gene051067	MS.gene52607	0.810913	9.10E-51	-1.69E-46
MS.gene051094	MS.gene52607	0.800304	1.53E-48	-1.69E-46
MS.gene05124	MS.gene52607	0.807931	3.97E-50	-1.69E-46
MS.gene051263	MS.gene52607	0.836748	7.80E-57	-1.69E-46
MS.gene051478	MS.gene52607	0.829898	3.98E-55	-1.69E-46
MS.gene051629	MS.gene52607	0.850712	1.42E-60	-1.69E-46
MS.gene051742	MS.gene52607	0.853127	2.93E-61	-1.69E-46
MS.gene051816	MS.gene52607	0.835183	1.95E-56	-1.69E-46
MS.gene051955	MS.gene52607	0.879618	1.08E-69	-1.69E-46
MS.gene052143	MS.gene52607	0.884612	1.65E-71	-1.69E-46
MS.gene052308	MS.gene52607	0.866874	2.07E-65	-1.69E-46
MS.gene052367	MS.gene52607	0.896621	3.10E-76	-1.69E-46
MS.gene053173	MS.gene52607	0.848547	5.72E-60	-1.69E-46
MS.gene053348	MS.gene52607	0.850042	2.19E-60	-1.69E-46
MS.gene053416	MS.gene52607	0.862864	3.75E-64	-1.69E-46
MS.gene053417	MS.gene52607	0.864868	8.92E-65	-1.69E-46
MS.gene05369	MS.gene52607	0.832024	1.20E-55	-1.69E-46
MS.gene053929	MS.gene52607	0.817623	3.00E-52	-1.69E-46
MS.gene054551	MS.gene52607	0.858686	6.95E-63	-1.69E-46
MS.gene054552	MS.gene52607	0.803825	2.90E-49	-1.69E-46
MS.gene054593	MS.gene52607	0.843468	1.38E-58	-1.69E-46
MS.gene054614	MS.gene52607	0.832684	8.22E-56	-1.69E-46
MS.gene054643	MS.gene52607	0.820502	6.63E-53	-1.69E-46
MS.gene055308	MS.gene52607	0.853384	2.48E-61	-1.69E-46
MS.gene055411	MS.gene52607	0.807181	5.73E-50	-1.69E-46
MS.gene055578	MS.gene52607	0.878591	2.48E-69	-1.69E-46
MS.gene055579	MS.gene52607	0.841593	4.32E-58	-1.69E-46
MS.gene055957	MS.gene52607	0.914359	2.10E-84	-1.69E-46
MS.gene056041	MS.gene52607	0.913552	5.39E-84	-1.69E-46
MS.gene056042	MS.gene52607	0.881897	1.64E-70	-1.69E-46
MS.gene056335	MS.gene52607	0.831504	1.61E-55	-1.69E-46
MS.gene05640	MS.gene52607	0.834439	3.00E-56	-1.69E-46
MS.gene056526	MS.gene52607	0.863256	2.83E-64	-1.69E-46
MS.gene056528	MS.gene52607	0.817101	3.93E-52	-1.69E-46
MS.gene057458	MS.gene52607	0.842204	2.98E-58	-1.69E-46
MS.gene057483	MS.gene52607	0.839622	1.42E-57	-1.69E-46
MS.gene058128	MS.gene52607	0.811947	5.43E-51	-1.69E-46
MS.gene058196	MS.gene52607	0.871898	4.81E-67	-1.69E-46
MS.gene058197	MS.gene52607	0.869049	4.14E-66	-1.69E-46
MS.gene058198	MS.gene52607	0.873261	1.69E-67	-1.69E-46
MS.gene058818	MS.gene52607	0.823732	1.18E-53	-1.69E-46
MS.gene058827	MS.gene52607	0.810985	8.78E-51	-1.69E-46
MS.gene058844	MS.gene52607	0.809691	1.67E-50	-1.69E-46
MS.gene058849	MS.gene52607	0.807547	4.79E-50	-1.69E-46
MS.gene058853	MS.gene52607	0.856491	3.10E-62	-1.69E-46
MS.gene059300	MS.gene52607	0.87254	2.94E-67	-1.69E-46
MS.gene059553	MS.gene52607	0.826984	2.01E-54	-1.69E-46
MS.gene060473	MS.gene52607	0.907594	4.28E-81	-1.69E-46
MS.gene060565	MS.gene52607	0.904867	7.83E-80	-1.69E-46
MS.gene06057	MS.gene52607	0.895289	1.10E-75	-1.69E-46
MS.gene060825	MS.gene52607	0.840851	6.78E-58	-1.69E-46
MS.gene06093	MS.gene52607	0.82485	6.45E-54	-1.69E-46
MS.gene061099	MS.gene52607	0.876467	1.36E-68	-1.69E-46
MS.gene061260	MS.gene52607	0.891544	3.61E-74	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52607.t1	MTR_7g080550	98.919	185	2	0	1	185	1	185	9.74e-136	377
MS.gene52607.t1	MTR_8g081530	70.286	175	45	2	14	185	4	174	7.32e-75	223

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52607.t1	AT5G16250	67.914	187	54	3	1	185	1	183	2.26e-80	237
MS.gene52607.t1	AT3G02640	66.845	187	58	3	1	185	1	185	1.96e-77	229
MS.gene52607.t1	AT5G36800	61.582	177	55	4	18	185	44	216	4.31e-68	207
MS.gene52607.t1	AT5G36710	61.582	177	55	4	18	185	44	216	4.31e-68	207
MS.gene52607.t1	AT5G36800	61.582	177	55	4	18	185	9	181	7.21e-68	205
MS.gene52607.t1	AT5G36710	61.582	177	55	4	18	185	9	181	7.21e-68	205

Find 40 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCATGTCTTCACCATTGC+TGG	0.213239	7.4:+25785596	MS.gene52607:CDS
ACCATTGCTGGAGCTGTTTC+AGG	0.221629	7.4:+25785608	MS.gene52607:CDS
TACTTGTAGGACTGTTGTTT+TGG	0.257403	7.4:+25785898	MS.gene52607:CDS
TTCTGCAACTATGTCCTCTT+AGG	0.263638	7.4:+25785497	MS.gene52607:CDS
GGATTGGCCATGGCCTTTCC+AGG	0.339796	7.4:+25785955	MS.gene52607:CDS
AGAGGACATAGTTGCAGAAA+AGG	0.386856	7.4:-25785493	None:intergenic
TGCAGAAAAGGAAGAGTTTG+TGG	0.401747	7.4:-25785481	None:intergenic
CCGTTGGTATTCTGCTCATA+TGG	0.404355	7.4:+25785652	MS.gene52607:CDS
AAGTATTATGCTTGTGTTGA+AGG	0.407930	7.4:+25785869	MS.gene52607:CDS
GTGAGTTGCAGTCTTATGTA+AGG	0.427295	7.4:+25785750	MS.gene52607:CDS
GTTTCAGGCTGTGCTGCTGT+TGG	0.438604	7.4:+25785623	MS.gene52607:CDS
TCAACAAGATGAAGATTTGA+AGG	0.442954	7.4:+25785934	MS.gene52607:CDS
TGACGAGGTGTGATTGTAGA+TGG	0.444409	7.4:-25785449	None:intergenic
TGAAGACATGGAGAAGGATA+AGG	0.461871	7.4:-25785586	None:intergenic
TGTCACCAATTCTTCAAACC+TGG	0.477395	7.4:-25785973	None:intergenic
AAGATGAAGATTTGAAGGAT+TGG	0.477591	7.4:+25785939	MS.gene52607:CDS
AGCTTGAAGCTGCACCTAAG+AGG	0.489271	7.4:-25785511	None:intergenic
AGGAAGAAGCCGCAGACGGA+AGG	0.533462	7.4:-25785566	None:intergenic
TTGAAGGTGGGAATACTTGT+AGG	0.538479	7.4:+25785885	MS.gene52607:CDS
CGCTTAGTCCCTTCCGTCTG+CGG	0.538763	7.4:+25785557	MS.gene52607:CDS
CTCCAGCAATGGTGAAGACA+TGG	0.539403	7.4:-25785598	None:intergenic
CTGCTGTTGGTGCTAACCGT+TGG	0.557004	7.4:+25785636	MS.gene52607:CDS
ATTATGCTTGTGTTGAAGGT+GGG	0.566434	7.4:+25785873	MS.gene52607:CDS
CCAATTCTTCAAACCTGGAA+AGG	0.571102	7.4:-25785968	None:intergenic
TATTATGCTTGTGTTGAAGG+TGG	0.573085	7.4:+25785872	MS.gene52607:CDS
TATGGTAGCTACTGTTCTAA+CGG	0.573662	7.4:+25785670	MS.gene52607:CDS
GGAAGAAGCCGCAGACGGAA+GGG	0.584820	7.4:-25785565	None:intergenic
GCCTGAAACAGCTCCAGCAA+TGG	0.585887	7.4:-25785609	None:intergenic
CTTCAAACCTGGAAAGGCCA+TGG	0.588475	7.4:-25785962	None:intergenic
GAGTCAAAACAACCCACTCA+AGG	0.600902	7.4:-25785832	None:intergenic
CGCAGACGGAAGGGACTAAG+CGG	0.602315	7.4:-25785556	None:intergenic
CTAAGCGGAGCGAAAGAGTG+AGG	0.610580	7.4:-25785541	None:intergenic
AAGATTTGAAGGATTGGCCA+TGG	0.611621	7.4:+25785945	MS.gene52607:CDS
TGAGTTGCAGTCTTATGTAA+GGG	0.621403	7.4:+25785751	MS.gene52607:CDS
CAATGGTGAAGACATGGAGA+AGG	0.622789	7.4:-25785592	None:intergenic
TCTTATGTAAGGGAAGAAGA+TGG	0.628672	7.4:+25785761	MS.gene52607:CDS
TTGTGGATGTTGTAGTGACG+AGG	0.630615	7.4:-25785464	None:intergenic
CGAGGTGTGATTGTAGATGG+AGG	0.643030	7.4:-25785446	None:intergenic
GATAAGGAAGAAGCCGCAGA+CGG	0.647152	7.4:-25785570	None:intergenic
CCATATGAGCAGAATACCAA+CGG	0.664716	7.4:-25785652	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGATGAAGATTTGAAGGAT+TGG	+	chr7.4:25785939-25785958	MS.gene52607:CDS	30.0%
	AAGTATTATGCTTGTGTTGA+AGG	+	chr7.4:25785869-25785888	MS.gene52607:CDS	30.0%
	TCAACAAGATGAAGATTTGA+AGG	+	chr7.4:25785934-25785953	MS.gene52607:CDS	30.0%
!!!	TTCTAACGGCTATTTTTCAA+GGG	+	chr7.4:25785684-25785703	MS.gene52607:CDS	30.0%
!!!	TTTCTGTTCTTGTTTTTACG+AGG	+	chr7.4:25785711-25785730	MS.gene52607:CDS	30.0%
	ATTATGCTTGTGTTGAAGGT+GGG	+	chr7.4:25785873-25785892	MS.gene52607:CDS	35.0%
	TATGGTAGCTACTGTTCTAA+CGG	+	chr7.4:25785670-25785689	MS.gene52607:CDS	35.0%
	TATTATGCTTGTGTTGAAGG+TGG	+	chr7.4:25785872-25785891	MS.gene52607:CDS	35.0%
	TCTTATGTAAGGGAAGAAGA+TGG	+	chr7.4:25785761-25785780	MS.gene52607:CDS	35.0%
!	TGAGTTGCAGTCTTATGTAA+GGG	+	chr7.4:25785751-25785770	MS.gene52607:CDS	35.0%
!	TTTGATCTTTTGCCTTGAGT+GGG	+	chr7.4:25785820-25785839	MS.gene52607:CDS	35.0%
!	TTTTGATCTTTTGCCTTGAG+TGG	+	chr7.4:25785819-25785838	MS.gene52607:CDS	35.0%
!!	TACTTGTAGGACTGTTGTTT+TGG	+	chr7.4:25785898-25785917	MS.gene52607:CDS	35.0%
!!!	ACTTGTAGGACTGTTGTTTT+GGG	+	chr7.4:25785899-25785918	MS.gene52607:CDS	35.0%
!!!	GTTCTAACGGCTATTTTTCA+AGG	+	chr7.4:25785683-25785702	MS.gene52607:CDS	35.0%
	AGAGGACATAGTTGCAGAAA+AGG	-	chr7.4:25785496-25785515	None:intergenic	40.0%
	CCATATGAGCAGAATACCAA+CGG	-	chr7.4:25785655-25785674	None:intergenic	40.0%
	TGAAGACATGGAGAAGGATA+AGG	-	chr7.4:25785589-25785608	None:intergenic	40.0%
	TGCAGAAAAGGAAGAGTTTG+TGG	-	chr7.4:25785484-25785503	None:intergenic	40.0%
	TTCTGCAACTATGTCCTCTT+AGG	+	chr7.4:25785497-25785516	MS.gene52607:CDS	40.0%
	TTGAAGGTGGGAATACTTGT+AGG	+	chr7.4:25785885-25785904	MS.gene52607:CDS	40.0%
!	GTGAGTTGCAGTCTTATGTA+AGG	+	chr7.4:25785750-25785769	MS.gene52607:CDS	40.0%
!!	AAGATTTGAAGGATTGGCCA+TGG	+	chr7.4:25785945-25785964	MS.gene52607:CDS	40.0%
!!!	AACGGCTATTTTTCAAGGGT+CGG	+	chr7.4:25785688-25785707	MS.gene52607:CDS	40.0%
!!!	TCAGTGATTTTGAAGCTGTC+TGG	+	chr7.4:25785785-25785804	MS.gene52607:CDS	40.0%
!!!	TGTTGTTTTGGGAAGTGCTA+AGG	+	chr7.4:25785910-25785929	MS.gene52607:CDS	40.0%
	CAATGGTGAAGACATGGAGA+AGG	-	chr7.4:25785595-25785614	None:intergenic	45.0%
	CCGTTGGTATTCTGCTCATA+TGG	+	chr7.4:25785652-25785671	MS.gene52607:CDS	45.0%
	GAGTCAAAACAACCCACTCA+AGG	-	chr7.4:25785835-25785854	None:intergenic	45.0%
	TGACGAGGTGTGATTGTAGA+TGG	-	chr7.4:25785452-25785471	None:intergenic	45.0%
	TTGTGGATGTTGTAGTGACG+AGG	-	chr7.4:25785467-25785486	None:intergenic	45.0%
!	ACGAGGACGAGTGATTTTCT+TGG	+	chr7.4:25785728-25785747	MS.gene52607:CDS	45.0%
!!!	GTGATTTTGAAGCTGTCTGG+TGG	+	chr7.4:25785788-25785807	MS.gene52607:CDS	45.0%
!!!	TGAGTGGGTTGTTTTGACTC+TGG	+	chr7.4:25785835-25785854	MS.gene52607:CDS	45.0%
	AGCTTGAAGCTGCACCTAAG+AGG	-	chr7.4:25785514-25785533	None:intergenic	50.0%
	CGAGGTGTGATTGTAGATGG+AGG	-	chr7.4:25785449-25785468	None:intergenic	50.0%
	CTCCAGCAATGGTGAAGACA+TGG	-	chr7.4:25785601-25785620	None:intergenic	50.0%
	CTCCATGTCTTCACCATTGC+TGG	+	chr7.4:25785596-25785615	MS.gene52607:CDS	50.0%
	GATAAGGAAGAAGCCGCAGA+CGG	-	chr7.4:25785573-25785592	None:intergenic	50.0%
!	ACCATTGCTGGAGCTGTTTC+AGG	+	chr7.4:25785608-25785627	MS.gene52607:CDS	50.0%
	CTAAGCGGAGCGAAAGAGTG+AGG	-	chr7.4:25785544-25785563	None:intergenic	55.0%
	GCCTGAAACAGCTCCAGCAA+TGG	-	chr7.4:25785612-25785631	None:intergenic	55.0%
!	GTTTCAGGCTGTGCTGCTGT+TGG	+	chr7.4:25785623-25785642	MS.gene52607:CDS	55.0%
!!	CTGCTGTTGGTGCTAACCGT+TGG	+	chr7.4:25785636-25785655	MS.gene52607:CDS	55.0%
	AGGAAGAAGCCGCAGACGGA+AGG	-	chr7.4:25785569-25785588	None:intergenic	60.0%
	CGCAGACGGAAGGGACTAAG+CGG	-	chr7.4:25785559-25785578	None:intergenic	60.0%
	CGCTTAGTCCCTTCCGTCTG+CGG	+	chr7.4:25785557-25785576	MS.gene52607:CDS	60.0%
	GGAAGAAGCCGCAGACGGAA+GGG	-	chr7.4:25785568-25785587	None:intergenic	60.0%
!!	GGATTGGCCATGGCCTTTCC+AGG	+	chr7.4:25785955-25785974	MS.gene52607:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	25785425	25785982	25785425	ID=MS.gene52607
chr7.4	mRNA	25785425	25785982	25785425	ID=MS.gene52607.t1;Parent=MS.gene52607
chr7.4	exon	25785425	25785982	25785425	ID=MS.gene52607.t1.exon1;Parent=MS.gene52607.t1
chr7.4	CDS	25785425	25785982	25785425	ID=cds.MS.gene52607.t1;Parent=MS.gene52607.t1

Gene Sequence

>MS.gene52607

ATGGGTCTCTCAATGAACAATCCTCCATCTACAATCACACCTCGTCACTACAACATCCACAAACTCTTCCTTTTCTGCAACTATGTCCTCTTAGGTGCAGCTTCAAGCTGTATCTTCCTCACTCTTTCGCTCCGCTTAGTCCCTTCCGTCTGCGGCTTCTTCCTTATCCTTCTCCATGTCTTCACCATTGCTGGAGCTGTTTCAGGCTGTGCTGCTGTTGGTGCTAACCGTTGGTATTCTGCTCATATGGTAGCTACTGTTCTAACGGCTATTTTTCAAGGGTCGGTTTCTGTTCTTGTTTTTACGAGGACGAGTGATTTTCTTGGTGAGTTGCAGTCTTATGTAAGGGAAGAAGATGGTTCAGTGATTTTGAAGCTGTCTGGTGGACTTGCTATTTTGATCTTTTGCCTTGAGTGGGTTGTTTTGACTCTGGCGTTTTTCTTGAAGTATTATGCTTGTGTTGAAGGTGGGAATACTTGTAGGACTGTTGTTTTGGGAAGTGCTAAGGTTCAACAAGATGAAGATTTGAAGGATTGGCCATGGCCTTTCCAGGTTTGA

Protein sequence

>MS.gene52607.t1

MGLSMNNPPSTITPRHYNIHKLFLFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHVFTIAGAVSGCAAVGANRWYSAHMVATVLTAIFQGSVSVLVFTRTSDFLGELQSYVREEDGSVILKLSGGLAILIFCLEWVVLTLAFFLKYYACVEGGNTCRTVVLGSAKVQQDEDLKDWPWPFQV