AlfalfaGEDB | MS.gene56303

M. sativa cultivar XinJiangDaYe / MS.gene56303

KEGG: K01179
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005975 GO:0008150 GO:0008152 GO:0016787 GO:0016798 GO:0044238 GO:0071704
PFAM: PF00759.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56303.t1	XP_003598326.1	85.4	418	5	2	15	376	67	484	3.40E-207	730.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56303.t1	Q9C9H5	65.5	415	86	3	15	373	66	479	4.3e-158	558.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56303.t1	G7IVD9	85.4	418	5	2	15	376	67	484	2.5e-207	730.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050338	MS.gene56303	0.838275	3.17E-57	-1.69E-46
MS.gene050707	MS.gene56303	0.810736	9.94E-51	-1.69E-46
MS.gene052383	MS.gene56303	0.800369	1.49E-48	-1.69E-46
MS.gene058382	MS.gene56303	0.80318	3.94E-49	-1.69E-46
MS.gene059699	MS.gene56303	0.813405	2.60E-51	-1.69E-46
MS.gene059703	MS.gene56303	0.816162	6.38E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene56303	MS.gene29940	PPI
MS.gene56303	MS.gene96428	PPI
MS.gene56303	MS.gene72272	PPI
MS.gene56303	MS.gene95200	PPI
MS.gene56303	MS.gene50877	PPI
MS.gene56303	MS.gene063734	PPI
MS.gene56303	MS.gene026232	PPI
MS.gene56303	MS.gene42640	PPI
MS.gene56303	MS.gene057924	PPI
MS.gene56303	MS.gene25810	PPI
MS.gene56303	MS.gene88560	PPI
MS.gene56303	MS.gene015691	PPI
MS.gene56303	MS.gene020482	PPI
MS.gene56303	MS.gene057923	PPI
MS.gene56303	MS.gene014960	PPI
MS.gene020483	MS.gene56303	PPI
MS.gene56303	MS.gene057880	PPI
MS.gene56303	MS.gene32754	PPI
MS.gene56303	MS.gene51810	PPI
MS.gene56303	MS.gene95919	PPI
MS.gene56303	MS.gene02701	PPI
MS.gene56303	MS.gene51667	PPI
MS.gene56303	MS.gene51960	PPI
MS.gene56303	MS.gene009506	PPI
MS.gene56303	MS.gene044195	PPI
MS.gene56303	MS.gene020485	PPI
MS.gene56303	MS.gene88559	PPI
MS.gene56303	MS.gene95918	PPI
MS.gene56303	MS.gene016750	PPI
MS.gene56303	MS.gene026233	PPI
MS.gene56303	MS.gene070498	PPI
MS.gene56303	MS.gene70730	PPI
MS.gene56303	MS.gene81610	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56303.t1	MTR_3g010330	85.407	418	5	2	15	376	67	484	0.0	728
MS.gene56303.t1	MTR_3g080810	48.931	421	154	4	14	374	76	495	7.12e-139	405
MS.gene56303.t1	MTR_1g088190	48.910	413	149	5	21	373	69	479	6.28e-133	389
MS.gene56303.t1	MTR_3g117500	47.754	423	156	7	16	375	67	487	4.44e-132	387
MS.gene56303.t1	MTR_8g104820	45.390	423	168	6	15	375	72	493	3.16e-124	367
MS.gene56303.t1	MTR_4g035975	45.585	419	164	7	14	372	75	489	2.43e-122	362
MS.gene56303.t1	MTR_4g035930	44.952	416	170	5	13	372	55	467	2.59e-122	362
MS.gene56303.t1	MTR_4g035955	45.192	416	169	5	13	372	54	466	5.99e-122	360
MS.gene56303.t1	MTR_5g010000	40.758	422	183	8	15	376	82	496	5.26e-105	318
MS.gene56303.t1	MTR_5g009970	39.007	423	191	8	12	374	6	421	3.36e-101	306
MS.gene56303.t1	MTR_5g009970	39.048	420	189	8	15	374	83	495	1.15e-99	305
MS.gene56303.t1	MTR_8g099410	38.785	428	190	10	15	374	70	493	1.67e-90	281
MS.gene56303.t1	MTR_8g099410	38.785	428	190	10	15	374	70	493	2.26e-89	281
MS.gene56303.t1	MTR_5g009970	37.157	401	185	8	34	374	1	394	2.07e-88	272
MS.gene56303.t1	MTR_1g037340	38.242	421	193	9	15	373	67	482	3.08e-87	272
MS.gene56303.t1	MTR_3g080190	36.385	426	201	8	15	372	82	505	9.64e-83	261
MS.gene56303.t1	MTR_0393s0050	87.143	140	3	1	231	355	20	159	1.77e-81	247
MS.gene56303.t1	MTR_4g074960	38.118	425	189	11	15	370	71	490	8.41e-79	254
MS.gene56303.t1	MTR_5g085730	35.047	428	204	10	15	372	73	496	1.09e-78	251
MS.gene56303.t1	MTR_5g093590	35.765	425	202	9	15	370	76	498	1.54e-78	250
MS.gene56303.t1	MTR_3g023740	36.930	417	197	8	15	373	72	480	2.73e-78	249
MS.gene56303.t1	MTR_3g105230	36.207	406	193	8	15	362	72	469	2.87e-77	246
MS.gene56303.t1	MTR_8g075990	35.363	427	197	13	15	370	104	522	3.35e-69	226
MS.gene56303.t1	MTR_3g113720	33.724	427	204	11	15	370	98	516	1.19e-68	225
MS.gene56303.t1	MTR_5g009970	39.858	281	146	5	111	374	52	326	2.39e-68	219
MS.gene56303.t1	MTR_1g088190	45.703	256	93	3	21	231	69	323	6.39e-68	218
MS.gene56303.t1	MTR_2g028480	35.135	444	194	18	16	375	159	592	2.79e-56	194
MS.gene56303.t1	MTR_7g108925	51.899	158	60	2	231	373	43	199	1.48e-50	169
MS.gene56303.t1	MTR_3g082440	33.028	436	200	19	17	369	162	588	3.66e-50	178
MS.gene56303.t1	MTR_3g090540	30.317	442	213	15	14	369	148	580	1.63e-42	157
MS.gene56303.t1	MTR_1g037320	38.650	163	54	4	34	153	1	160	3.33e-24	101
MS.gene56303.t1	MTR_1g037320	35.772	123	63	2	263	370	168	289	3.60e-15	75.5
MS.gene56303.t1	MTR_4g128810	26.506	332	168	11	58	369	55	330	8.68e-18	84.3
MS.gene56303.t1	MTR_7g108920	50.847	59	25	1	141	195	86	144	8.77e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56303.t1	AT1G71380	65.228	417	88	3	14	374	65	480	0.0	558
MS.gene56303.t1	AT1G22880	63.876	418	94	3	14	375	65	481	0.0	545
MS.gene56303.t1	AT1G22880	62.312	398	93	3	34	375	1	397	1.76e-179	504
MS.gene56303.t1	AT4G02290	51.069	421	144	5	13	373	92	510	1.62e-145	422
MS.gene56303.t1	AT1G02800	51.185	422	142	6	12	373	82	499	1.61e-143	417
MS.gene56303.t1	AT4G02290	49.500	400	140	5	34	373	1	398	1.78e-132	385
MS.gene56303.t1	AT1G70710	46.919	422	162	5	15	376	70	489	7.08e-132	387
MS.gene56303.t1	AT1G23210	46.318	421	164	5	15	375	70	488	1.90e-127	375
MS.gene56303.t1	AT4G39010	44.811	424	170	6	15	375	71	493	2.28e-120	357
MS.gene56303.t1	AT1G22880	65.637	259	48	1	14	231	65	323	5.90e-120	351
MS.gene56303.t1	AT4G39000	40.845	426	188	6	13	375	65	489	2.24e-109	329
MS.gene56303.t1	AT4G23560	41.587	416	175	9	15	370	65	472	1.53e-102	311
MS.gene56303.t1	AT4G09740	40.767	417	177	10	15	370	65	472	9.57e-99	301
MS.gene56303.t1	AT4G38990	38.283	431	197	7	13	375	63	492	1.34e-97	299
MS.gene56303.t1	AT4G39010	43.533	317	131	4	15	283	71	387	1.10e-89	275
MS.gene56303.t1	AT3G43860	38.717	421	192	8	12	374	73	485	1.64e-87	273
MS.gene56303.t1	AT3G43860	38.717	421	192	8	12	374	73	485	1.64e-87	273
MS.gene56303.t1	AT2G32990	37.264	424	196	9	15	370	81	502	1.59e-85	269
MS.gene56303.t1	AT2G44540	37.232	419	197	9	14	370	74	488	2.04e-85	268
MS.gene56303.t1	AT1G64390	37.705	427	194	10	15	373	67	489	5.09e-85	270
MS.gene56303.t1	AT2G44550	36.842	418	199	8	14	370	74	487	1.38e-84	265
MS.gene56303.t1	AT2G44570	37.352	423	198	10	14	373	74	492	8.21e-84	263
MS.gene56303.t1	AT4G11050	37.028	424	195	11	15	370	68	487	1.24e-82	262
MS.gene56303.t1	AT4G11050	37.028	424	195	11	15	370	68	487	4.02e-82	263
MS.gene56303.t1	AT2G44560	37.915	422	196	9	14	373	74	491	4.14e-82	259
MS.gene56303.t1	AT4G11050	37.028	424	195	11	15	370	68	487	4.24e-82	263
MS.gene56303.t1	AT1G75680	38.221	416	182	11	15	363	98	505	3.34e-78	250
MS.gene56303.t1	AT1G48930	37.736	424	192	9	15	370	72	491	3.95e-77	250
MS.gene56303.t1	AT1G19940	35.476	420	196	11	15	367	89	500	6.83e-75	241
MS.gene56303.t1	AT1G19940	35.476	420	196	11	15	367	89	500	6.83e-75	241
MS.gene56303.t1	AT4G38990	39.146	281	132	5	13	262	63	335	1.02e-60	199
MS.gene56303.t1	AT4G24260	33.710	442	201	16	16	374	156	588	8.12e-56	193
MS.gene56303.t1	AT2G44570	36.145	332	159	10	14	296	74	401	1.22e-55	188
MS.gene56303.t1	AT1G19940	35.093	322	154	8	15	284	89	407	1.22e-54	186
MS.gene56303.t1	AT5G49720	34.763	443	196	16	16	374	155	588	5.16e-54	188
MS.gene56303.t1	AT1G65610	31.881	436	207	19	17	370	161	588	2.46e-44	162
MS.gene56303.t1	AT4G24260	33.728	338	150	14	16	284	156	488	1.27e-35	136
MS.gene56303.t1	AT4G24260	34.457	267	108	10	16	217	156	420	1.86e-33	129

Find 86 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAACAGTTCTCAAGGTATT+TGG	0.188395	3.1:+5422437	None:intergenic
ATGGCTCCAACCAATATATT+AGG	0.202587	3.1:+5422145	None:intergenic
TGCTGTTAAATACTACAATT+TGG	0.250375	3.1:-5423141	MS.gene56303:CDS
CTACAATTTGGCCAAGTCTT+TGG	0.251872	3.1:-5423129	MS.gene56303:CDS
CCAGAATATGAGCAATAAAA+TGG	0.291892	3.1:+5423554	None:intergenic
TACAATTTGGCCAAGTCTTT+GGG	0.295167	3.1:-5423128	MS.gene56303:CDS
CTGGTTTGGACCTCCAACTA+TGG	0.301617	3.1:+5422126	None:intergenic
CAACATTTGTGATCAACATT+TGG	0.314931	3.1:+5423809	None:intergenic
TTATCCCAGCTGAAGATATC+AGG	0.329473	3.1:+5423092	None:intergenic
TGAATCCTCTTGGGAAAATA+TGG	0.350676	3.1:+5422265	None:intergenic
TCATGGAGCACAATTGAATA+TGG	0.352249	3.1:-5423947	MS.gene56303:CDS
GAATCAGAACCCTAATATAT+TGG	0.355685	3.1:-5422155	MS.gene56303:CDS
AATCTAACATTAATTGTTAC+CGG	0.359003	3.1:+5422009	None:intergenic
TACAACCTATTCCAAGTATA+TGG	0.367719	3.1:-5422504	MS.gene56303:CDS
TAGGGTTCTGATTCATTGAA+TGG	0.377070	3.1:+5422164	None:intergenic
CATCAGGAAATCCGTCGTTC+TGG	0.380884	3.1:+5422107	None:intergenic
CTTTGTAGCTGCCATATACT+TGG	0.384398	3.1:+5422493	None:intergenic
GGAAATCCGTCGTTCTGGTT+TGG	0.384940	3.1:+5422112	None:intergenic
TACATCAATGGTGGGATTGT+TGG	0.387929	3.1:-5422049	MS.gene56303:CDS
GCTCTTGCTTCTTTCATTTG+TGG	0.396038	3.1:+5423911	None:intergenic
TCCTCTGTGGTGAATCCTCT+TGG	0.412922	3.1:+5422255	None:intergenic
ACACCGACATAGAGTCTTCC+AGG	0.415923	3.1:+5423839	None:intergenic
CTATAGTCACCGCGATCATC+AGG	0.416806	3.1:+5422091	None:intergenic
CAGTTTGCTTTGCAGTATCA+AGG	0.418073	3.1:-5423611	MS.gene56303:CDS
CCACCATTGATGTAAGTTGC+TGG	0.419287	3.1:+5422058	None:intergenic
TTGCTCATATTCTGGATTCA+AGG	0.420522	3.1:-5423546	MS.gene56303:intron
TGGCTCCAACCAATATATTA+GGG	0.420525	3.1:+5422146	None:intergenic
GGACCTTTAGCGTACTTTGC+CGG	0.422505	3.1:-5422028	MS.gene56303:CDS
ACATACTGAAGATTGCTTGC+AGG	0.422983	3.1:+5422548	None:intergenic
CTAACAGTGTCCATATCTTC+TGG	0.423308	3.1:+5423779	None:intergenic
TTGGGAAAGACCAGAAGATA+TGG	0.432588	3.1:-5423789	MS.gene56303:CDS
CAAATGTTGATCACAAATGT+TGG	0.436805	3.1:-5423808	MS.gene56303:CDS
GTGTTAGCACTTTGTTCCAT+TGG	0.437623	3.1:+5424073	None:intergenic
TGTAGTTGAAGAGGGTGAAT+TGG	0.441166	3.1:+5422800	None:intergenic
TCAGTTCATCCACAGACAAT+AGG	0.447266	3.1:-5422214	MS.gene56303:CDS
AGCTGCCATATACTTGGAAT+AGG	0.451707	3.1:+5422499	None:intergenic
ATATTGGTTGGAGCCATAGT+TGG	0.454285	3.1:-5422139	MS.gene56303:CDS
AATTGAATATGGAAAGAGAA+TGG	0.462216	3.1:-5423936	MS.gene56303:CDS
ATCAACCTGATATCTTCAGC+TGG	0.462559	3.1:-5423097	MS.gene56303:CDS
AAATGTTGATCACAAATGTT+GGG	0.467634	3.1:-5423807	MS.gene56303:CDS
TCTTCAGGATGAACTATTGT+GGG	0.468517	3.1:-5423192	MS.gene56303:intron
GTGCACATGTCCTTCTCTCA+AGG	0.474161	3.1:-5423058	MS.gene56303:CDS
ATTGAATATGGAAAGAGAAT+GGG	0.486477	3.1:-5423935	MS.gene56303:CDS
ATTGTTGTTGTGAATGCCAT+TGG	0.494336	3.1:+5423974	None:intergenic
ACAGGAGGCTATTATGATGC+AGG	0.500795	3.1:-5424019	MS.gene56303:CDS
ATCTGTGGCGTGGCGAATTG+CGG	0.500987	3.1:+5423889	None:intergenic
CCTCTGTGGTGAATCCTCTT+GGG	0.506991	3.1:+5422256	None:intergenic
TCACAACAACAATGTTATCA+TGG	0.509771	3.1:-5423964	MS.gene56303:CDS
CAGAACCCTAATATATTGGT+TGG	0.511456	3.1:-5422151	MS.gene56303:CDS
ATCTTCAGGATGAACTATTG+TGG	0.519125	3.1:-5423193	MS.gene56303:intron
ATCATCAGCTCCCAAAGACT+TGG	0.531480	3.1:+5423118	None:intergenic
GTAGTTGAAGAGGGTGAATT+GGG	0.534837	3.1:+5422801	None:intergenic
TTGGTTGGAGCCATAGTTGG+AGG	0.545842	3.1:-5422136	MS.gene56303:CDS
CTACAGAGAGCATTGCTGAA+TGG	0.545991	3.1:-5422882	MS.gene56303:intron
CCACAGACAATAGGATGTGA+TGG	0.547733	3.1:-5422205	MS.gene56303:CDS
GAACCAGCAACTTACATCAA+TGG	0.548177	3.1:-5422061	MS.gene56303:CDS
AGCTGGGATAACAAATATGC+TGG	0.549315	3.1:-5423080	MS.gene56303:CDS
GAGAACTGTTGCGAAAAGAC+AGG	0.553255	3.1:-5422426	MS.gene56303:CDS
GGAAGACTCTATGTCGGTGT+TGG	0.560843	3.1:-5423836	MS.gene56303:CDS
TTCAACTTACCCTTGAGAGA+AGG	0.570746	3.1:+5423048	None:intergenic
TTGCAGGTGGATTTGACAGG+AGG	0.571495	3.1:-5424034	MS.gene56303:CDS
TTACCGGCAAAGTACGCTAA+AGG	0.575384	3.1:+5422025	None:intergenic
AACTATTGTGGGGAGCTGCA+TGG	0.591973	3.1:-5423181	MS.gene56303:CDS
TGCACATGTCCTTCTCTCAA+GGG	0.598418	3.1:-5423057	MS.gene56303:intron
CCATCACATCCTATTGTCTG+TGG	0.600403	3.1:+5422205	None:intergenic
GGCTCATACAGTGATTCACT+TGG	0.605518	3.1:-5423590	MS.gene56303:CDS
TCAACCTGATATCTTCAGCT+GGG	0.606032	3.1:-5423096	MS.gene56303:CDS
TGTATACTGTGTAGTTGAAG+AGG	0.614209	3.1:+5422791	None:intergenic
CCAGCAACTTACATCAATGG+TGG	0.615251	3.1:-5422058	MS.gene56303:CDS
AAGCACACATTTAACTGTGG+TGG	0.619659	3.1:-5422473	MS.gene56303:CDS
GTGTTGATGTTGCACACTTG+AGG	0.620658	3.1:+5423861	None:intergenic
AAGTGTGCAACATCAACACC+TGG	0.627700	3.1:-5423857	MS.gene56303:CDS
CAGCAACTTACATCAATGGT+GGG	0.637988	3.1:-5422057	MS.gene56303:CDS
CTTCAGGATGAACTATTGTG+GGG	0.648937	3.1:-5423191	MS.gene56303:intron
GTATACTGTGTAGTTGAAGA+GGG	0.651354	3.1:+5422792	None:intergenic
TGAGGAGATAATCTGTGGCG+TGG	0.651976	3.1:+5423879	None:intergenic
CAGACAATAGGATGTGATGG+AGG	0.672550	3.1:-5422202	MS.gene56303:CDS
ACACCTGGAAGACTCTATGT+CGG	0.686227	3.1:-5423842	MS.gene56303:CDS
ACACTTGAGGAGATAATCTG+TGG	0.686648	3.1:+5423874	None:intergenic
CCCAAGAGGATTCACCACAG+AGG	0.688585	3.1:-5422256	MS.gene56303:CDS
TCAACTACACAGTATACACA+AGG	0.701067	3.1:-5422786	MS.gene56303:intron
TGTGGATGAACTGATAGTGA+AGG	0.707912	3.1:+5422223	None:intergenic
GACGGATTTCCTGATGATCG+CGG	0.711969	3.1:-5422100	MS.gene56303:CDS
GGAGGTCCAAACCAGAACGA+CGG	0.712912	3.1:-5422118	MS.gene56303:CDS
ACAAAGCACACATTTAACTG+TGG	0.729394	3.1:-5422476	MS.gene56303:CDS
AAGGCAATGAAGATCCTCTG+TGG	0.790898	3.1:+5422242	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACATAAATTTCAAATCAATA+TGG	-	chr3.1:5423442-5423461	MS.gene56303:intron	15.0%
!!	ACTATAAATATAATAGTTGA+TGG	+	chr3.1:5423372-5423391	None:intergenic	15.0%
!!!	AAAATTTGTTGTAAAATTTC+AGG	-	chr3.1:5423744-5423763	MS.gene56303:intron	15.0%
!!!	ATAAAATCAATTTTAAACAG+AGG	-	chr3.1:5422766-5422785	MS.gene56303:intron	15.0%
!!!	TAAAATCAATTTTAAACAGA+GGG	-	chr3.1:5422767-5422786	MS.gene56303:intron	15.0%
!!!	TATAGTTAAATATCTAGTTT+TGG	-	chr3.1:5422631-5422650	MS.gene56303:intron	15.0%
!!	AGTTTAGACAAAAAACAAAT+TGG	+	chr3.1:5423088-5423107	None:intergenic	20.0%
!!	ATAAAAACTTTGATCAGTAT+AGG	-	chr3.1:5423231-5423250	MS.gene56303:intron	20.0%
!!!	AACAAATTTTATCAACAAAG+AGG	+	chr3.1:5423734-5423753	None:intergenic	20.0%
!	AAATGTTGATCACAAATGTT+GGG	-	chr3.1:5422281-5422300	MS.gene56303:intron	25.0%
!	AACAAAAGCTTAGAATAAGT+TGG	+	chr3.1:5422435-5422454	None:intergenic	25.0%
!	AATAGGCATTATTTATCTTC+AGG	-	chr3.1:5422881-5422900	MS.gene56303:intron	25.0%
!	AATTGAATATGGAAAGAGAA+TGG	-	chr3.1:5422152-5422171	MS.gene56303:CDS	25.0%
!	ATGAAATCATTCTCTATTCT+TGG	+	chr3.1:5423408-5423427	None:intergenic	25.0%
!	ATTGAATATGGAAAGAGAAT+GGG	-	chr3.1:5422153-5422172	MS.gene56303:CDS	25.0%
!	TCTCTCTAATAGTAAGAAAT+AGG	+	chr3.1:5422833-5422852	None:intergenic	25.0%
!	TGATTCATTGAATGGAAAAA+CGG	+	chr3.1:5423919-5423938	None:intergenic	25.0%
!	TGCTGTTAAATACTACAATT+TGG	-	chr3.1:5422947-5422966	MS.gene56303:intron	25.0%
!!	GGCTTATAGAGTATTATAAA+GGG	-	chr3.1:5423463-5423482	MS.gene56303:intron	25.0%
!!	TGGCTTATAGAGTATTATAA+AGG	-	chr3.1:5423462-5423481	MS.gene56303:intron	25.0%
!!	TGTGAAATTCAATTTTCCAA+TGG	-	chr3.1:5422098-5422117	MS.gene56303:CDS	25.0%
!!	TTTCAGGTAGACTATATATT+AGG	-	chr3.1:5423760-5423779	MS.gene56303:intron	25.0%
!!!	TTTTGGTAGTATCACTATTA+GGG	-	chr3.1:5422648-5422667	MS.gene56303:intron	25.0%
	ATTGCTTGAATTAATGTGCA+GGG	-	chr3.1:5423499-5423518	MS.gene56303:intron	30.0%
	CAAATGTTGATCACAAATGT+TGG	-	chr3.1:5422280-5422299	MS.gene56303:intron	30.0%
	CAACATTTGTGATCAACATT+TGG	+	chr3.1:5422282-5422301	None:intergenic	30.0%
	CCAGAATATGAGCAATAAAA+TGG	+	chr3.1:5422537-5422556	None:intergenic	30.0%
	GAATCAGAACCCTAATATAT+TGG	-	chr3.1:5423933-5423952	MS.gene56303:CDS	30.0%
	TACAACCTATTCCAAGTATA+TGG	-	chr3.1:5423584-5423603	MS.gene56303:CDS	30.0%
	TATGTATCTTCAAACAATCC+CGG	-	chr3.1:5422342-5422361	MS.gene56303:intron	30.0%
	TATTGCTTGAATTAATGTGC+AGG	-	chr3.1:5423498-5423517	MS.gene56303:intron	30.0%
	TCACAACAACAATGTTATCA+TGG	-	chr3.1:5422124-5422143	MS.gene56303:CDS	30.0%
	TCTACTTGTATCGTTCTAAT+AGG	-	chr3.1:5422864-5422883	MS.gene56303:CDS	30.0%
!	CCATTTTATTGCTCATATTC+TGG	-	chr3.1:5422534-5422553	MS.gene56303:CDS	30.0%
!	CTTATTCTAAGCTTTTGTTG+AGG	-	chr3.1:5422436-5422455	MS.gene56303:CDS	30.0%
!	TTGGTTGTTAAGTTTGAGAT+CGG	-	chr3.1:5422723-5422742	MS.gene56303:intron	30.0%
!	TTTGGTAGTATCACTATTAG+GGG	-	chr3.1:5422649-5422668	MS.gene56303:intron	30.0%
!!	ATACACAAACATTGTTTTGC+AGG	-	chr3.1:5422038-5422057	MS.gene56303:CDS	30.0%
!!	GTTTTGGTAGTATCACTATT+AGG	-	chr3.1:5422647-5422666	MS.gene56303:intron	30.0%
	ACAAAGCACACATTTAACTG+TGG	-	chr3.1:5423612-5423631	MS.gene56303:CDS	35.0%
	ACCATATAGGACATTCTAAG+TGG	+	chr3.1:5423793-5423812	None:intergenic	35.0%
	ATCTTCAGGATGAACTATTG+TGG	-	chr3.1:5422895-5422914	MS.gene56303:intron	35.0%
	ATGGCTCCAACCAATATATT+AGG	+	chr3.1:5423946-5423965	None:intergenic	35.0%
	ATGTCCTATATGGTAGGTTA+TGG	-	chr3.1:5423799-5423818	MS.gene56303:CDS	35.0%
	CAGAACCCTAATATATTGGT+TGG	-	chr3.1:5423937-5423956	MS.gene56303:CDS	35.0%
	CTTAGAATGTCCTATATGGT+AGG	-	chr3.1:5423793-5423812	MS.gene56303:CDS	35.0%
	GTATACTGTGTAGTTGAAGA+GGG	+	chr3.1:5423299-5423318	None:intergenic	35.0%
	TACAATTTGGCCAAGTCTTT+GGG	-	chr3.1:5422960-5422979	MS.gene56303:intron	35.0%
	TCAACTACACAGTATACACA+AGG	-	chr3.1:5423302-5423321	MS.gene56303:intron	35.0%
	TCATGGAGCACAATTGAATA+TGG	-	chr3.1:5422141-5422160	MS.gene56303:CDS	35.0%
	TCCACTTAGAATGTCCTATA+TGG	-	chr3.1:5423789-5423808	MS.gene56303:CDS	35.0%
	TCTTCAGGATGAACTATTGT+GGG	-	chr3.1:5422896-5422915	MS.gene56303:intron	35.0%
	TGAATCCTCTTGGGAAAATA+TGG	+	chr3.1:5423826-5423845	None:intergenic	35.0%
	TGGCTCCAACCAATATATTA+GGG	+	chr3.1:5423945-5423964	None:intergenic	35.0%
	TGTATACTGTGTAGTTGAAG+AGG	+	chr3.1:5423300-5423319	None:intergenic	35.0%
	TTGCTCATATTCTGGATTCA+AGG	-	chr3.1:5422542-5422561	MS.gene56303:CDS	35.0%
	TTGCTTGAATTAATGTGCAG+GGG	-	chr3.1:5423500-5423519	MS.gene56303:intron	35.0%
!	ATTGTTGTTGTGAATGCCAT+TGG	+	chr3.1:5422117-5422136	None:intergenic	35.0%
!	TAGGGTTCTGATTCATTGAA+TGG	+	chr3.1:5423927-5423946	None:intergenic	35.0%
!	TTACACGTAGCAGACAATTT+TGG	-	chr3.1:5422704-5422723	MS.gene56303:intron	35.0%
	AAGCACACATTTAACTGTGG+TGG	-	chr3.1:5423615-5423634	MS.gene56303:CDS	40.0%
	ACACTTGAGGAGATAATCTG+TGG	+	chr3.1:5422217-5422236	None:intergenic	40.0%
	ACATACTGAAGATTGCTTGC+AGG	+	chr3.1:5423543-5423562	None:intergenic	40.0%
	AGCTGCCATATACTTGGAAT+AGG	+	chr3.1:5423592-5423611	None:intergenic	40.0%
	AGCTGGGATAACAAATATGC+TGG	-	chr3.1:5423008-5423027	MS.gene56303:intron	40.0%
	ATCAACCTGATATCTTCAGC+TGG	-	chr3.1:5422991-5423010	MS.gene56303:intron	40.0%
	CAGCAACTTACATCAATGGT+GGG	-	chr3.1:5424031-5424050	MS.gene56303:CDS	40.0%
	CCAATACTCGGATTCTTACT+CGG	-	chr3.1:5422604-5422623	MS.gene56303:intron	40.0%
	CTAACAGTGTCCATATCTTC+TGG	+	chr3.1:5422312-5422331	None:intergenic	40.0%
	CTACAATTTGGCCAAGTCTT+TGG	-	chr3.1:5422959-5422978	MS.gene56303:intron	40.0%
	CTTCAGGATGAACTATTGTG+GGG	-	chr3.1:5422897-5422916	MS.gene56303:intron	40.0%
	CTTTGTAGCTGCCATATACT+TGG	+	chr3.1:5423598-5423617	None:intergenic	40.0%
	GAACCAGCAACTTACATCAA+TGG	-	chr3.1:5424027-5424046	MS.gene56303:CDS	40.0%
	GCTCTTGCTTCTTTCATTTG+TGG	+	chr3.1:5422180-5422199	None:intergenic	40.0%
	GTAGTTGAAGAGGGTGAATT+GGG	+	chr3.1:5423290-5423309	None:intergenic	40.0%
	TAGTATCACTATTAGGGGTC+TGG	-	chr3.1:5422654-5422673	MS.gene56303:intron	40.0%
	TCAACCTGATATCTTCAGCT+GGG	-	chr3.1:5422992-5423011	MS.gene56303:intron	40.0%
	TCAGTTCATCCACAGACAAT+AGG	-	chr3.1:5423874-5423893	MS.gene56303:CDS	40.0%
	TGCTTGAATTAATGTGCAGG+GGG	-	chr3.1:5423501-5423520	MS.gene56303:intron	40.0%
	TGGACCATAACCTACCATAT+AGG	+	chr3.1:5423806-5423825	None:intergenic	40.0%
	TGTAGTTGAAGAGGGTGAAT+TGG	+	chr3.1:5423291-5423310	None:intergenic	40.0%
	TTATCCCAGCTGAAGATATC+AGG	+	chr3.1:5422999-5423018	None:intergenic	40.0%
	TTCAACTTACCCTTGAGAGA+AGG	+	chr3.1:5423043-5423062	None:intergenic	40.0%
	TTGGGAAAGACCAGAAGATA+TGG	-	chr3.1:5422299-5422318	MS.gene56303:intron	40.0%
	TTTGTTGAGGACTTCACAGA+AGG	-	chr3.1:5422449-5422468	MS.gene56303:CDS	40.0%
!	ATATTGGTTGGAGCCATAGT+TGG	-	chr3.1:5423949-5423968	MS.gene56303:CDS	40.0%
!	ATGGTCCATATTTTCCCAAG+AGG	-	chr3.1:5423818-5423837	MS.gene56303:CDS	40.0%
!	CAGTTTGCTTTGCAGTATCA+AGG	-	chr3.1:5422477-5422496	MS.gene56303:CDS	40.0%
!	CTGTGTGATACAGTTTTTCG+AGG	-	chr3.1:5422804-5422823	MS.gene56303:CDS	40.0%
!	GCAACAGTTCTCAAGGTATT+TGG	+	chr3.1:5423654-5423673	None:intergenic	40.0%
!	TACATCAATGGTGGGATTGT+TGG	-	chr3.1:5424039-5424058	MS.gene56303:CDS	40.0%
!	TCTTTTCGCAACAGTTCTCA+AGG	+	chr3.1:5423661-5423680	None:intergenic	40.0%
!	TGTGGATGAACTGATAGTGA+AGG	+	chr3.1:5423868-5423887	None:intergenic	40.0%
!!	CACAAACATTGTTTTGCAGG+TGG	-	chr3.1:5422041-5422060	MS.gene56303:CDS	40.0%
	AAGGCAATGAAGATCCTCTG+TGG	+	chr3.1:5423849-5423868	None:intergenic	45.0%
	AAGTGTGCAACATCAACACC+TGG	-	chr3.1:5422231-5422250	MS.gene56303:CDS	45.0%
	ACACCTGGAAGACTCTATGT+CGG	-	chr3.1:5422246-5422265	MS.gene56303:CDS	45.0%
	ACAGGAGGCTATTATGATGC+AGG	-	chr3.1:5422069-5422088	MS.gene56303:CDS	45.0%
	ATCATCAGCTCCCAAAGACT+TGG	+	chr3.1:5422973-5422992	None:intergenic	45.0%
	CAGACAATAGGATGTGATGG+AGG	-	chr3.1:5423886-5423905	MS.gene56303:CDS	45.0%
	CCACAGACAATAGGATGTGA+TGG	-	chr3.1:5423883-5423902	MS.gene56303:CDS	45.0%
	CCACCATTGATGTAAGTTGC+TGG	+	chr3.1:5424033-5424052	None:intergenic	45.0%
	CCAGCAACTTACATCAATGG+TGG	-	chr3.1:5424030-5424049	MS.gene56303:CDS	45.0%
	CCATCACATCCTATTGTCTG+TGG	+	chr3.1:5423886-5423905	None:intergenic	45.0%
	CCGAGTAAGAATCCGAGTAT+TGG	+	chr3.1:5422607-5422626	None:intergenic	45.0%
	GAGAACTGTTGCGAAAAGAC+AGG	-	chr3.1:5423662-5423681	MS.gene56303:intron	45.0%
	GGCTCATACAGTGATTCACT+TGG	-	chr3.1:5422498-5422517	MS.gene56303:CDS	45.0%
	GGGTCTGGATTCTCTAATGT+AGG	-	chr3.1:5422669-5422688	MS.gene56303:intron	45.0%
	TGCACATGTCCTTCTCTCAA+GGG	-	chr3.1:5423031-5423050	MS.gene56303:intron	45.0%
	TTACCGGCAAAGTACGCTAA+AGG	+	chr3.1:5424066-5424085	None:intergenic	45.0%
	TTCAGCAGCAACATCAGAAC+CGG	+	chr3.1:5422364-5422383	None:intergenic	45.0%
!	CCTAGAGCATCTCCAATACT+CGG	-	chr3.1:5422592-5422611	MS.gene56303:intron	45.0%
!	GTGTTGATGTTGCACACTTG+AGG	+	chr3.1:5422230-5422249	None:intergenic	45.0%
!!	CTACAGAGAGCATTGCTGAA+TGG	-	chr3.1:5423149-5423168	MS.gene56303:CDS	45.0%
!!	GTTTTGCAGGTGGATTTGAC+AGG	-	chr3.1:5422051-5422070	MS.gene56303:CDS	45.0%
	AACTATTGTGGGGAGCTGCA+TGG	-	chr3.1:5422907-5422926	MS.gene56303:intron	50.0%
	ACACCGACATAGAGTCTTCC+AGG	+	chr3.1:5422252-5422271	None:intergenic	50.0%
	CATCAGGAAATCCGTCGTTC+TGG	+	chr3.1:5423984-5424003	None:intergenic	50.0%
	CCTCTGTGGTGAATCCTCTT+GGG	+	chr3.1:5423835-5423854	None:intergenic	50.0%
	CTATAGTCACCGCGATCATC+AGG	+	chr3.1:5424000-5424019	None:intergenic	50.0%
	GGAAGACTCTATGTCGGTGT+TGG	-	chr3.1:5422252-5422271	MS.gene56303:CDS	50.0%
	GGACCTTTAGCGTACTTTGC+CGG	-	chr3.1:5424060-5424079	MS.gene56303:CDS	50.0%
	GTGCACATGTCCTTCTCTCA+AGG	-	chr3.1:5423030-5423049	MS.gene56303:intron	50.0%
	TCAGCAGCAACATCAGAACC+GGG	+	chr3.1:5422363-5422382	None:intergenic	50.0%
	TCCTCTGTGGTGAATCCTCT+TGG	+	chr3.1:5423836-5423855	None:intergenic	50.0%
	TGAGGAGATAATCTGTGGCG+TGG	+	chr3.1:5422212-5422231	None:intergenic	50.0%
	TTGGTTGGAGCCATAGTTGG+AGG	-	chr3.1:5423952-5423971	MS.gene56303:CDS	50.0%
!	GACGGATTTCCTGATGATCG+CGG	-	chr3.1:5423988-5424007	MS.gene56303:CDS	50.0%
!	GGAAATCCGTCGTTCTGGTT+TGG	+	chr3.1:5423979-5423998	None:intergenic	50.0%
!	GGGAGCTGCATGGCTTTTTA+GGG	-	chr3.1:5422917-5422936	MS.gene56303:intron	50.0%
!	TTGCAGGTGGATTTGACAGG+AGG	-	chr3.1:5422054-5422073	MS.gene56303:CDS	50.0%
!!	CCGAGTATTGGAGATGCTCT+AGG	+	chr3.1:5422595-5422614	None:intergenic	50.0%
!!	CTGGTTTGGACCTCCAACTA+TGG	+	chr3.1:5423965-5423984	None:intergenic	50.0%
	ATCTGTGGCGTGGCGAATTG+CGG	+	chr3.1:5422202-5422221	None:intergenic	55.0%
	CCCAAGAGGATTCACCACAG+AGG	-	chr3.1:5423832-5423851	MS.gene56303:CDS	55.0%
	GGAGGTCCAAACCAGAACGA+CGG	-	chr3.1:5423970-5423989	MS.gene56303:CDS	55.0%
!	AACTGCTGCTGCACTTGCTG+CGG	-	chr3.1:5422383-5422402	MS.gene56303:intron	55.0%
!	GGGGAGCTGCATGGCTTTTT+AGG	-	chr3.1:5422916-5422935	MS.gene56303:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	5422018	5424092	5422018	ID=MS.gene56303
chr3.1	mRNA	5422018	5424092	5422018	ID=MS.gene56303.t1;Parent=MS.gene56303
chr3.1	exon	5423766	5424092	5423766	ID=MS.gene56303.t1.exon1;Parent=MS.gene56303.t1
chr3.1	CDS	5423766	5424092	5423766	ID=cds.MS.gene56303.t1;Parent=MS.gene56303.t1
chr3.1	exon	5423547	5423642	5423547	ID=MS.gene56303.t1.exon2;Parent=MS.gene56303.t1
chr3.1	CDS	5423547	5423642	5423547	ID=cds.MS.gene56303.t1;Parent=MS.gene56303.t1
chr3.1	exon	5423058	5423207	5423058	ID=MS.gene56303.t1.exon3;Parent=MS.gene56303.t1
chr3.1	CDS	5423058	5423207	5423058	ID=cds.MS.gene56303.t1;Parent=MS.gene56303.t1
chr3.1	exon	5422787	5422898	5422787	ID=MS.gene56303.t1.exon4;Parent=MS.gene56303.t1
chr3.1	CDS	5422787	5422898	5422787	ID=cds.MS.gene56303.t1;Parent=MS.gene56303.t1
chr3.1	exon	5422423	5422590	5422423	ID=MS.gene56303.t1.exon5;Parent=MS.gene56303.t1
chr3.1	CDS	5422423	5422590	5422423	ID=cds.MS.gene56303.t1;Parent=MS.gene56303.t1
chr3.1	exon	5422018	5422295	5422018	ID=MS.gene56303.t1.exon6;Parent=MS.gene56303.t1
chr3.1	CDS	5422018	5422295	5422018	ID=cds.MS.gene56303.t1;Parent=MS.gene56303.t1

Gene Sequence

>MS.gene56303

ATGGAACAAAGTGCTAACACATACACAAACATTGTTTTGCAGGTGGATTTGACAGGAGGCTATTATGATGCAGGAGACAATGTGAAATTCAATTTTCCAATGGCATTCACAACAACAATGTTATCATGGAGCACAATTGAATATGGAAAGAGAATGGGACCACAAATGAAAGAAGCAAGAGCCGCAATTCGCCACGCCACAGATTATCTCCTCAAGTGTGCAACATCAACACCTGGAAGACTCTATGTCGGTGTTGGAGATCCAAATGTTGATCACAAATGTTGGGAAAGACCAGAAGATATGGACACTGTTAGAACTGTTTATTATGTATCTTCAAACAATCCCGGTTCTGATGTTGCTGCTGAAACTGCTGCTGCACTTGCTGCGGCGTCTATTGTTTTTAGAAAAGTTGATCCAACTTATTCTAAGCTTTTGTTGAGGACTTCACAGAAGGTTTATCAGTTTGCTTTGCAGTATCAAGGCTCATACAGTGATTCACTTGGTTCTGCTGTTTGTCCATTTTATTGCTCATATTCTGGATTCAAGGTAAAATTTATTTTCTACTGAGTTTTAACCTAGAGCATCTCCAATACTCGGATTCTTACTCGGTTCGTATAGTTAAATATCTAGTTTTGGTAGTATCACTATTAGGGGTCTGGATTCTCTAATGTAGGAGAATCACACATTTACACGTAGCAGACAATTTTGGTTGTTAAGTTTGAGATCGGACATCTCAGATCACACATTCATAAAATCAATTTTAAACAGAGGGTCAAAATCACTAAACTGTGTGATACAGTTTTTCGAGGCAACCTATTTCTTACTATTAGAGAGAGTTGATGTGATTCTACTTGTATCGTTCTAATAGGCATTATTTATCTTCAGGATGAACTATTGTGGGGAGCTGCATGGCTTTTTAGGGCAACAAATGCTGTTAAATACTACAATTTGGCCAAGTCTTTGGGAGCTGATGATCAACCTGATATCTTCAGCTGGGATAACAAATATGCTGGTGCACATGTCCTTCTCTCAAGGGTAAGTTGAATTTCATTGAAAAATATGAATAACCAATTTGTTTTTTGTCTAAACTTTGCTGATTCACTAGTTTTCTATTGTTGTTTTTAATTGTTACTACAGAGAGCATTGCTGAATGGTGATAAAAATTTCTATTGTTGTTTTTAATTGTTACTACAGAGAGCATTGCTGAATGGTGATAAAAACTTTGATCAGTATAGGCAAGAAGCTGATGATTTCATGTGCAAGATCTTGCCCAATTCACCCTCTTCAACTACACAGTATACACAAGGTAAAAGCAACATGACTATCAGCTATTATAATTCGAAAAATGTTACCATCAACTATTATATTTATAGTATTCTAAGTGATTCCAAGAATAGAGAATGATTTCATGAAAATGTAAAATGACATAAATTTCAAATCAATATGGCTTATAGAGTATTATAAAGGGCGTTGTATCATGTATTGCTTGAATTAATGTGCAGGGGGACTAATGTTCAAGTTACCTGCAAGCAATCTTCAGTATGTGACATCTATAACATTCTTACTTACAACCTATTCCAAGTATATGGCAGCTACAAAGCACACATTTAACTGTGGTGGCGTCTTTGTCACTCCAAATACCTTGAGAACTGTTGCGAAAAGACAGGCAAGTCTTATTATTATTTTAACAAAAAAGTTGTAACAATCTCTTCTCCTCTTTGTTGATAAAATTTGTTGTAAAATTTCAGGTAGACTATATATTAGGTGAAAATCCACTTAGAATGTCCTATATGGTAGGTTATGGTCCATATTTTCCCAAGAGGATTCACCACAGAGGATCTTCATTGCCTTCACTATCAGTTCATCCACAGACAATAGGATGTGATGGAGGTTTCAATCCGTTTTTCCATTCAATGAATCAGAACCCTAATATATTGGTTGGAGCCATAGTTGGAGGTCCAAACCAGAACGACGGATTTCCTGATGATCGCGGTGACTATAGTCATTCAGAACCAGCAACTTACATCAATGGTGGGATTGTTGGACCTTTAGCGTACTTTGCCGGTAACAATTAA

Protein sequence

>MS.gene56303.t1

MEQSANTYTNIVLQVDLTGGYYDAGDNVKFNFPMAFTTTMLSWSTIEYGKRMGPQMKEARAAIRHATDYLLKCATSTPGRLYVGVGDPNVDHKCWERPEDMDTVRTVYYKVYQFALQYQGSYSDSLGSAVCPFYCSYSGFKDELLWGAAWLFRATNAVKYYNLAKSLGADDQPDIFSWDNKYAGAHVLLSRRALLNGDKNFDQYRQEADDFMCKILPNSPSSTTQYTQGGLMFKLPASNLQYVTSITFLLTTYSKYMAATKHTFNCGGVFVTPNTLRTVAKRQASYGPYFPKRIHHRGSSLPSLSVHPQTIGCDGGFNPFFHSMNQNPNILVGAIVGGPNQNDGFPDDRGDYSHSEPATYINGGIVGPLAYFAGNN