AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32754

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32754.t1 MTR_3g105230 92.547 483 15 2 1 462 1 483 0.0 928
MS.gene32754.t1 MTR_3g023740 84.679 483 53 2 1 462 1 483 0.0 840
MS.gene32754.t1 MTR_5g009970 46.035 454 221 6 29 458 40 493 2.87e-140 412
MS.gene32754.t1 MTR_5g010000 46.154 455 221 5 28 458 38 492 2.76e-136 402
MS.gene32754.t1 MTR_5g085730 41.884 499 252 8 1 461 1 499 4.35e-128 381
MS.gene32754.t1 MTR_3g080190 43.006 479 236 6 21 462 31 509 2.00e-127 380
MS.gene32754.t1 MTR_8g099410 43.923 469 226 7 27 459 25 492 6.31e-127 378
MS.gene32754.t1 MTR_3g080810 43.696 460 223 8 30 456 35 491 1.04e-126 377
MS.gene32754.t1 MTR_8g099410 43.923 469 226 7 27 459 25 492 3.21e-125 377
MS.gene32754.t1 MTR_5g093590 41.785 493 248 6 8 461 11 503 4.39e-125 373
MS.gene32754.t1 MTR_8g104820 42.208 462 229 9 30 456 30 488 1.28e-122 366
MS.gene32754.t1 MTR_3g117500 42.082 461 231 8 30 456 24 482 2.17e-120 360
MS.gene32754.t1 MTR_5g009970 44.444 414 206 6 69 458 6 419 1.11e-118 354
MS.gene32754.t1 MTR_3g010330 41.416 466 238 6 25 459 20 481 4.63e-117 352
MS.gene32754.t1 MTR_4g035975 43.100 471 222 12 30 460 27 491 1.67e-116 351
MS.gene32754.t1 MTR_4g035955 41.575 457 233 7 29 456 13 464 4.50e-115 346
MS.gene32754.t1 MTR_4g074960 41.365 469 237 8 29 460 27 494 4.89e-114 348
MS.gene32754.t1 MTR_4g035930 40.611 458 236 7 29 456 14 465 6.95e-113 341
MS.gene32754.t1 MTR_1g037340 41.957 460 235 6 30 459 25 482 1.93e-109 332
MS.gene32754.t1 MTR_1g088190 42.333 463 212 9 30 459 39 479 4.90e-109 331
MS.gene32754.t1 MTR_5g009970 42.857 392 200 6 91 458 1 392 2.86e-106 321
MS.gene32754.t1 MTR_3g113720 38.806 469 242 9 31 460 58 520 4.37e-105 323
MS.gene32754.t1 MTR_8g075990 37.179 468 251 9 31 460 64 526 8.43e-90 283
MS.gene32754.t1 MTR_5g009970 44.136 324 168 4 148 458 1 324 1.29e-86 268
MS.gene32754.t1 MTR_2g028480 35.378 489 248 12 30 459 111 590 4.60e-81 263
MS.gene32754.t1 MTR_3g090540 33.058 484 258 12 32 458 109 583 5.26e-75 246
MS.gene32754.t1 MTR_3g082440 34.216 491 250 15 30 460 116 593 1.38e-72 241
MS.gene32754.t1 MTR_1g088190 41.776 304 124 7 30 301 39 321 1.05e-59 199
MS.gene32754.t1 MTR_1g111340 47.879 165 42 3 236 390 1 131 6.11e-39 137
MS.gene32754.t1 MTR_7g108925 43.709 151 83 2 310 459 50 199 1.91e-35 131
MS.gene32754.t1 MTR_1g037320 40.104 192 99 3 91 267 1 191 9.70e-28 112
MS.gene32754.t1 MTR_1g037320 41.803 122 69 2 336 456 169 289 1.59e-23 100
MS.gene32754.t1 MTR_4g128810 26.651 424 203 17 43 460 14 335 1.52e-27 113
MS.gene32754.t1 MTR_0393s0050 43.077 130 73 1 309 437 26 155 3.04e-27 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32754.t1 AT3G43860 65.152 462 139 3 21 460 24 485 0.0 620
MS.gene32754.t1 AT3G43860 65.152 462 139 3 21 460 24 485 0.0 620
MS.gene32754.t1 AT4G11050 45.182 467 219 7 30 460 26 491 1.14e-131 391
MS.gene32754.t1 AT4G11050 45.182 467 219 7 30 460 26 491 5.09e-131 392
MS.gene32754.t1 AT4G11050 45.182 467 219 7 30 460 26 491 5.28e-131 392
MS.gene32754.t1 AT4G23560 46.696 454 217 6 31 460 24 476 1.93e-129 383
MS.gene32754.t1 AT4G09740 46.272 456 220 6 29 460 22 476 1.39e-128 381
MS.gene32754.t1 AT4G02290 44.805 462 220 7 30 459 52 510 9.42e-128 380
MS.gene32754.t1 AT1G64390 44.325 467 223 7 30 460 25 490 1.98e-127 383
MS.gene32754.t1 AT1G70710 42.191 493 246 10 1 460 1 487 5.49e-125 372
MS.gene32754.t1 AT1G02800 45.238 462 216 8 30 459 43 499 8.20e-125 372
MS.gene32754.t1 AT2G32990 42.245 490 241 7 10 457 14 503 8.26e-124 370
MS.gene32754.t1 AT1G23210 41.856 485 242 10 9 460 10 487 5.09e-121 362
MS.gene32754.t1 AT1G71380 41.213 478 247 10 14 460 6 480 1.58e-116 350
MS.gene32754.t1 AT1G22880 41.957 460 235 9 30 460 24 480 3.87e-115 347
MS.gene32754.t1 AT2G44550 40.702 484 253 8 3 456 8 487 5.65e-114 344
MS.gene32754.t1 AT4G39010 39.831 472 247 9 24 460 23 492 4.67e-112 340
MS.gene32754.t1 AT2G44540 40.417 480 248 8 10 458 18 490 3.57e-111 337
MS.gene32754.t1 AT1G19940 41.253 463 223 9 30 453 48 500 1.17e-109 334
MS.gene32754.t1 AT1G19940 41.253 463 223 9 30 453 48 500 1.17e-109 334
MS.gene32754.t1 AT2G44560 40.204 490 258 8 1 459 6 491 3.22e-109 332
MS.gene32754.t1 AT2G44570 40.333 481 250 9 11 459 17 492 4.37e-106 324
MS.gene32754.t1 AT1G48930 40.645 465 239 7 29 457 29 492 4.33e-105 326
MS.gene32754.t1 AT1G75680 40.969 454 227 10 31 449 58 505 1.86e-101 313
MS.gene32754.t1 AT4G39000 37.554 466 254 7 30 460 25 488 3.24e-100 309
MS.gene32754.t1 AT4G38990 37.120 493 263 11 4 456 2 487 1.44e-97 302
MS.gene32754.t1 AT4G02290 41.646 401 199 7 91 459 1 398 9.37e-95 292
MS.gene32754.t1 AT1G22880 39.348 399 210 9 91 460 1 396 4.70e-88 275
MS.gene32754.t1 AT4G39010 37.500 368 195 7 24 357 23 389 2.94e-83 262
MS.gene32754.t1 AT5G49720 35.992 489 245 14 30 459 108 587 7.57e-81 262
MS.gene32754.t1 AT1G19940 39.344 366 177 7 30 356 48 407 1.49e-76 245
MS.gene32754.t1 AT2G44570 38.992 377 194 8 11 356 17 388 7.48e-73 235
MS.gene32754.t1 AT4G24260 35.510 490 246 14 30 459 108 587 2.66e-72 240
MS.gene32754.t1 AT1G65610 34.216 491 253 16 30 462 116 594 8.48e-70 233
MS.gene32754.t1 AT1G22880 43.000 300 141 7 30 301 24 321 4.89e-67 218
MS.gene32754.t1 AT4G38990 36.336 333 166 6 30 335 23 336 4.65e-57 192
MS.gene32754.t1 AT4G24260 32.558 387 195 12 30 356 108 488 6.77e-42 155
MS.gene32754.t1 AT4G24260 37.405 262 128 7 30 259 108 365 4.22e-39 147

Find 103 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAGCAAAGCTTGCTTCTT+TGG 0.183970 3.1:-83078290 MS.gene32754:CDS
CTCCAAGGAACCCTGTTATT+TGG 0.186545 3.1:+83080477 None:intergenic
ACCATGTATGATCTTCCTTC+TGG 0.191808 3.1:+83078562 None:intergenic
TGAATGTAGGTCGACCTATC+TGG 0.258745 3.1:-83080197 MS.gene32754:intron
TAACAGATGGATTACATATT+GGG 0.264521 3.1:-83078592 MS.gene32754:intron
CCCTTCTACTGCTCCTATTC+AGG 0.265491 3.1:-83079573 MS.gene32754:CDS
TACCAGGAGACAAATGTATT+TGG 0.265919 3.1:+83079180 None:intergenic
TGGATTCTAAAAGAGTATAT+TGG 0.281538 3.1:-83080629 MS.gene32754:CDS
CTAGCAAAGCTTGCTTCTTT+GGG 0.282416 3.1:-83078289 MS.gene32754:CDS
GCAGGAGATAATGTGAAATA+TGG 0.284440 3.1:-83080161 MS.gene32754:CDS
GTTACTATCAATTACTTTAA+CGG 0.311656 3.1:+83079911 None:intergenic
TTAACAGATGGATTACATAT+TGG 0.314396 3.1:-83078593 MS.gene32754:intron
AAAATCCCTTATATTCTTAG+AGG 0.322178 3.1:-83080523 MS.gene32754:CDS
CCATGTATGATCTTCCTTCT+GGG 0.323883 3.1:+83078563 None:intergenic
TCTCCTCAACAAAGCAAAAT+TGG 0.327119 3.1:-83079796 MS.gene32754:intron
CCAGTTAGCTCATGTGGATT+TGG 0.335953 3.1:+83078418 None:intergenic
GAAAACTCCCTCCAAATAAC+AGG 0.336359 3.1:-83080488 MS.gene32754:CDS
TTTGTGCCTCTAAGAATATA+AGG 0.344488 3.1:+83080517 None:intergenic
ATTTGAAGCAACAAAGGAAA+TGG 0.364952 3.1:-83080078 MS.gene32754:CDS
CCAAATCCACATGAGCTAAC+TGG 0.370400 3.1:-83078418 MS.gene32754:CDS
GCCTGAATAGGAGCAGTAGA+AGG 0.378073 3.1:+83079572 None:intergenic
TCGAAATGGCTGCAAAGAAA+TGG 0.380962 3.1:-83078442 MS.gene32754:CDS
AGATCATACATGGTAGGATT+CGG 0.394514 3.1:-83078553 MS.gene32754:CDS
CAACAATGATGATCATCAAC+TGG 0.395874 3.1:+83079966 None:intergenic
GGATTCTAAAAGAGTATATT+GGG 0.397703 3.1:-83080628 MS.gene32754:CDS
ATTTGGTGGTAAGGACTATC+AGG 0.398032 3.1:+83079197 None:intergenic
GATTTGGCAAAATCGCATAA+AGG 0.402232 3.1:-83079615 MS.gene32754:CDS
GATCATACATGGTAGGATTC+GGG 0.419888 3.1:-83078552 MS.gene32754:CDS
TCGAATTTGTCATTTATGTC+AGG 0.420508 3.1:+83078379 None:intergenic
TATTCAAGATGCAATCAAAT+GGG 0.427350 3.1:-83080051 MS.gene32754:CDS
TATGTGCAAGGTTCAGTCTT+AGG 0.428923 3.1:+83078337 None:intergenic
CTCCAAATAACAGGGTTCCT+TGG 0.434846 3.1:-83080479 MS.gene32754:CDS
GAATGTAGGTCGACCTATCT+GGG 0.442886 3.1:-83080196 MS.gene32754:intron
GAGCTAACTGGAGCCATTGT+AGG 0.443297 3.1:-83078406 MS.gene32754:CDS
GACAAATGTATTTGGTGGTA+AGG 0.445589 3.1:+83079188 None:intergenic
TTGTGCCTCTAAGAATATAA+GGG 0.447754 3.1:+83080518 None:intergenic
GGACAATTGATATCTTCGTT+TGG 0.451331 3.1:+83078475 None:intergenic
TTCTGAAACGGCTGCTGCTA+TGG 0.452071 3.1:-83079868 MS.gene32754:CDS
ACATACGTTATAGCCTGAAT+AGG 0.452957 3.1:+83079560 None:intergenic
AGGAAGCATGATACTTGTCC+AGG 0.461040 3.1:-83080574 MS.gene32754:CDS
TCTTCGTTTGGTGACAACTT+TGG 0.462848 3.1:+83078487 None:intergenic
TTCTTAGAGGCACAAAGATC+AGG 0.468999 3.1:-83080510 MS.gene32754:CDS
ATATTCAAGATGCAATCAAA+TGG 0.469316 3.1:-83080052 MS.gene32754:CDS
AGTTGATGATCATCATTGTT+GGG 0.470055 3.1:-83079964 MS.gene32754:CDS
GGAGACTCGGCAGTCGATGA+TGG 0.481663 3.1:-83080453 MS.gene32754:CDS
TCGCATAAAGGAACATATGA+TGG 0.482052 3.1:-83079603 MS.gene32754:CDS
GGACAAGTATCATGCTTCCT+TGG 0.482582 3.1:+83080577 None:intergenic
TCACTGTGACTACATTATCA+TGG 0.484072 3.1:-83080124 MS.gene32754:CDS
TTTGAAGCAACAAAGGAAAT+GGG 0.485003 3.1:-83080077 MS.gene32754:CDS
TTCAGAAGCAATCTCACTAC+CGG 0.492191 3.1:+83079884 None:intergenic
CAGTTGATGATCATCATTGT+TGG 0.500741 3.1:-83079965 MS.gene32754:CDS
TATGTTAATTCTCTTGCAGC+TGG 0.501079 3.1:-83078316 MS.gene32754:CDS
CACCAAATACATTTGTCTCC+TGG 0.502545 3.1:-83079182 MS.gene32754:intron
CAGGAGATAATGTGAAATAT+GGG 0.504345 3.1:-83080160 MS.gene32754:CDS
CAAGGAACCCTGTTATTTGG+AGG 0.510003 3.1:+83080480 None:intergenic
GGGTCAATTTAATTATAAAG+AGG 0.516477 3.1:-83080553 MS.gene32754:CDS
CTTTATAATTAAATTGACCC+TGG 0.518556 3.1:+83080556 None:intergenic
CAGCCATTTCGAAAAGCTCG+TGG 0.520074 3.1:+83078453 None:intergenic
CTATGATGAGCTTGTGTGGG+TGG 0.520571 3.1:+83078523 None:intergenic
ACCTCTATGATGAGCTTGTG+TGG 0.521792 3.1:+83078519 None:intergenic
TAGTGAGATTGCTTCTGAAA+CGG 0.528733 3.1:-83079880 MS.gene32754:CDS
CATGTAGGATGAATTGTTGT+GGG 0.533902 3.1:-83079426 MS.gene32754:intron
CCCAGAAGGAAGATCATACA+TGG 0.534406 3.1:-83078563 MS.gene32754:CDS
CAGGAGACAAATGTATTTGG+TGG 0.535513 3.1:+83079183 None:intergenic
GGATTTATTCATATGAGAGA+TGG 0.536941 3.1:-83079077 MS.gene32754:CDS
CACTGTGACTACATTATCAT+GGG 0.541514 3.1:-83080123 MS.gene32754:CDS
AATTGTTGTGGGCAGCAACT+TGG 0.542150 3.1:-83079415 MS.gene32754:CDS
CTTGAAACATTTAAAGCACA+TGG 0.547835 3.1:-83079245 MS.gene32754:CDS
CCTGAATAGGAGCAGTAGAA+GGG 0.550036 3.1:+83079573 None:intergenic
ATGGCTCCAGTTAGCTCATG+TGG 0.553897 3.1:+83078412 None:intergenic
GCATGGCTTACTTTGTTCCA+AGG 0.555429 3.1:-83080594 MS.gene32754:CDS
GGCAGCAACTTGGTTATACA+TGG 0.560142 3.1:-83079405 MS.gene32754:CDS
ATCAGTATGTCTTAAAACAA+TGG 0.562446 3.1:+83079836 None:intergenic
GCCGGTAGCATATTGTGTAT+TGG 0.563729 3.1:+83079052 None:intergenic
CCTCTATGATGAGCTTGTGT+GGG 0.566191 3.1:+83078520 None:intergenic
AACAGAATTTGAAGCAACAA+AGG 0.567677 3.1:-83080084 MS.gene32754:CDS
GCCAATACACAATATGCTAC+CGG 0.569100 3.1:-83079053 MS.gene32754:CDS
GGTTCCTTGGAGAGGAGACT+CGG 0.579042 3.1:-83080466 MS.gene32754:CDS
AAGGAACCCTGTTATTTGGA+GGG 0.579113 3.1:+83080481 None:intergenic
CCCACACAAGCTCATCATAG+AGG 0.580704 3.1:-83078520 MS.gene32754:CDS
GTAGTCACAGTGAATGCCAT+TGG 0.585430 3.1:+83080134 None:intergenic
AACAGATGGATTACATATTG+GGG 0.589442 3.1:-83078591 MS.gene32754:intron
ATTGGGCAATCATTGTAGCA+TGG 0.598810 3.1:-83080611 MS.gene32754:CDS
CTAACTGGAGCCATTGTAGG+TGG 0.599511 3.1:-83078403 MS.gene32754:CDS
GAAGGAAGATCATACATGGT+AGG 0.609021 3.1:-83078559 MS.gene32754:CDS
CTGAATAGGAGCAGTAGAAG+GGG 0.613296 3.1:+83079574 None:intergenic
AGTGAACAAGAAAGATGTGC+CGG 0.615391 3.1:+83079034 None:intergenic
TATGTCAGGTCCACCTACAA+TGG 0.616354 3.1:+83078393 None:intergenic
TGTGAAATATGGGCTACCAA+TGG 0.622156 3.1:-83080150 MS.gene32754:CDS
TCATGTAGGATGAATTGTTG+TGG 0.627857 3.1:-83079427 MS.gene32754:intron
AGAGAATTAACATATGTGCA+AGG 0.635433 3.1:+83078325 None:intergenic
ACTGCCGAGTCTCCTCTCCA+AGG 0.640954 3.1:+83080462 None:intergenic
AATGTAGGTCGACCTATCTG+GGG 0.642954 3.1:-83080195 MS.gene32754:intron
TCAGAAGCAATCTCACTACC+GGG 0.644101 3.1:+83079885 None:intergenic
AATAACAGGGTTCCTTGGAG+AGG 0.653619 3.1:-83080474 MS.gene32754:CDS
AGCCATTTCGAAAAGCTCGT+GGG 0.656326 3.1:+83078454 None:intergenic
ATTCAAGATGCAATCAAATG+GGG 0.657597 3.1:-83080050 MS.gene32754:CDS
AACAATGATGATCATCAACT+GGG 0.657794 3.1:+83079967 None:intergenic
GGAAGCATGATACTTGTCCA+GGG 0.658370 3.1:-83080573 MS.gene32754:CDS
GGTGGTAAGGACTATCAGGA+AGG 0.672514 3.1:+83079201 None:intergenic
AAAACTCCCTCCAAATAACA+GGG 0.697948 3.1:-83080487 MS.gene32754:CDS
GTAATTGATAGTAACACACC+CGG 0.711099 3.1:-83079903 MS.gene32754:CDS
ATGTAGGTCGACCTATCTGG+GGG 0.712955 3.1:-83080194 MS.gene32754:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATACATTAAATTAATAATT+TGG - chr3.1:83078670-83078689 MS.gene32754:intron 10.0%
!! TTTATATTATCAAACAAATT+TGG - chr3.1:83080035-83080054 MS.gene32754:CDS 10.0%
!!! TTTAACTTTTTGTTTATATT+TGG + chr3.1:83079926-83079945 None:intergenic 10.0%
!! TATAAACAAAAAGTTAAATG+TGG - chr3.1:83079928-83079947 MS.gene32754:CDS 15.0%
!!! AATTTTATTGAATTGAATGT+AGG - chr3.1:83078705-83078724 MS.gene32754:intron 15.0%
!! AAGAAACAAATTATATGTTG+AGG - chr3.1:83078918-83078937 MS.gene32754:intron 20.0%
!! AATTAATATGTGAGCATAAA+TGG + chr3.1:83080016-83080035 None:intergenic 20.0%
!! ATATTATCAAACAAATTTGG+AGG - chr3.1:83080038-83080057 MS.gene32754:CDS 20.0%
!! ATTATATATGATGATTATGC+AGG - chr3.1:83079814-83079833 MS.gene32754:CDS 20.0%
!! CATGATTAAAATATGTTAGA+CGG + chr3.1:83078647-83078666 None:intergenic 20.0%
!! CTGAATTTAAATATAAGCAA+AGG + chr3.1:83079162-83079181 None:intergenic 20.0%
!! GTTACTATCAATTACTTTAA+CGG + chr3.1:83079007-83079026 None:intergenic 20.0%
!! TAAACGTATATATATTTACC+AGG + chr3.1:83079754-83079773 None:intergenic 20.0%
!! TAATTGATTGTACATATCTT+TGG - chr3.1:83079463-83079482 MS.gene32754:intron 20.0%
!! TTATCAAAGCTTAGTAAATA+AGG + chr3.1:83079404-83079423 None:intergenic 20.0%
!!! AGTATACACTATATCAATTT+TGG + chr3.1:83080249-83080268 None:intergenic 20.0%
! AAAATCCCTTATATTCTTAG+AGG - chr3.1:83078392-83078411 MS.gene32754:CDS 25.0%
! AACAAATTATATGTTGAGGT+AGG - chr3.1:83078922-83078941 MS.gene32754:intron 25.0%
! ACAAATTATATGTTGAGGTA+GGG - chr3.1:83078923-83078942 MS.gene32754:intron 25.0%
! ATATGAGACTGAATTAATGT+TGG - chr3.1:83079432-83079451 MS.gene32754:intron 25.0%
! ATATTCAAGATGCAATCAAA+TGG - chr3.1:83078863-83078882 MS.gene32754:intron 25.0%
! ATCAGTATGTCTTAAAACAA+TGG + chr3.1:83079082-83079101 None:intergenic 25.0%
! CTTTATAATTAAATTGACCC+TGG + chr3.1:83078362-83078381 None:intergenic 25.0%
! GGGTCAATTTAATTATAAAG+AGG - chr3.1:83078362-83078381 MS.gene32754:CDS 25.0%
! TAGACGGAAAATAATGAATT+TGG + chr3.1:83078631-83078650 None:intergenic 25.0%
! TAGCGTGAACATATAATATT+TGG - chr3.1:83080295-83080314 MS.gene32754:intron 25.0%
! TATGAGACTGAATTAATGTT+GGG - chr3.1:83079433-83079452 MS.gene32754:intron 25.0%
! TATTCAAGATGCAATCAAAT+GGG - chr3.1:83078864-83078883 MS.gene32754:intron 25.0%
!! ATCTAAAGTCTTTATTACTG+CGG - chr3.1:83080196-83080215 MS.gene32754:intron 25.0%
!! ATGAATTTGGTCATCAATTT+TGG + chr3.1:83078618-83078637 None:intergenic 25.0%
!! GAAAATAGCCAATAGTATTT+TGG - chr3.1:83079222-83079241 MS.gene32754:CDS 25.0%
!! GGATTCTAAAAGAGTATATT+GGG - chr3.1:83078287-83078306 MS.gene32754:CDS 25.0%
!! TGGATTCTAAAAGAGTATAT+TGG - chr3.1:83078286-83078305 MS.gene32754:CDS 25.0%
!!! ATATTTGGTTTGTTAACAGA+TGG - chr3.1:83080310-83080329 MS.gene32754:intron 25.0%
!!! CAATAGTATTTTGGTTACAT+GGG - chr3.1:83079231-83079250 MS.gene32754:CDS 25.0%
!!! CTTTTTGTCTTCATTTTTTC+TGG + chr3.1:83078982-83079001 None:intergenic 25.0%
!!! TAACAGATGGATTACATATT+GGG - chr3.1:83080323-83080342 MS.gene32754:intron 25.0%
!!! TTAACAGATGGATTACATAT+TGG - chr3.1:83080322-83080341 MS.gene32754:intron 25.0%
AACAATGATGATCATCAACT+GGG + chr3.1:83078951-83078970 None:intergenic 30.0%
AGAGAATTAACATATGTGCA+AGG + chr3.1:83080593-83080612 None:intergenic 30.0%
ATATATGATGATTATGCAGG+TGG - chr3.1:83079817-83079836 MS.gene32754:CDS 30.0%
ATTCAAGATGCAATCAAATG+GGG - chr3.1:83078865-83078884 MS.gene32754:intron 30.0%
CAAATTATATGTTGAGGTAG+GGG - chr3.1:83078924-83078943 MS.gene32754:intron 30.0%
CAGGAGATAATGTGAAATAT+GGG - chr3.1:83078755-83078774 MS.gene32754:intron 30.0%
CCATGTAACCAAAATACTAT+TGG + chr3.1:83079233-83079252 None:intergenic 30.0%
GGATTTATTCATATGAGAGA+TGG - chr3.1:83079838-83079857 MS.gene32754:CDS 30.0%
TAATGTAGCAGAATTCAGTT+GGG - chr3.1:83079585-83079604 MS.gene32754:CDS 30.0%
TCAACAAAGCAAAATTGGTA+TGG - chr3.1:83079124-83079143 MS.gene32754:intron 30.0%
TTGGAATTTCTGACATTTAG+AGG + chr3.1:83078599-83078618 None:intergenic 30.0%
! AACAGAATTTGAAGCAACAA+AGG - chr3.1:83078831-83078850 MS.gene32754:intron 30.0%
! ATTTGAAGCAACAAAGGAAA+TGG - chr3.1:83078837-83078856 MS.gene32754:intron 30.0%
! CAATTTTGGATCTTCTATGA+TGG + chr3.1:83080235-83080254 None:intergenic 30.0%
! CTTGAAACATTTAAAGCACA+TGG - chr3.1:83079670-83079689 MS.gene32754:intron 30.0%
! GAAGTATTTGTTACATGCAT+AGG - chr3.1:83079184-83079203 MS.gene32754:CDS 30.0%
! TCGAATTTGTCATTTATGTC+AGG + chr3.1:83080539-83080558 None:intergenic 30.0%
! TCTAAAGTCTTTATTACTGC+GGG - chr3.1:83080197-83080216 MS.gene32754:intron 30.0%
! TTGTGCCTCTAAGAATATAA+GGG + chr3.1:83078400-83078419 None:intergenic 30.0%
! TTTGAAGCAACAAAGGAAAT+GGG - chr3.1:83078838-83078857 MS.gene32754:intron 30.0%
! TTTGTGCCTCTAAGAATATA+AGG + chr3.1:83078401-83078420 None:intergenic 30.0%
!! AACAGATGGATTACATATTG+GGG - chr3.1:83080324-83080343 MS.gene32754:intron 30.0%
!! ACATATCTTTGGAATCATGT+AGG - chr3.1:83079474-83079493 MS.gene32754:intron 30.0%
!! AGTTGATGATCATCATTGTT+GGG - chr3.1:83078951-83078970 MS.gene32754:CDS 30.0%
!! ATACCAATTTTGCTTTGTTG+AGG + chr3.1:83079125-83079144 None:intergenic 30.0%
!! CTACATTTTGAAGGTCAAAA+AGG - chr3.1:83079646-83079665 MS.gene32754:intron 30.0%
!!! CCAATAGTATTTTGGTTACA+TGG - chr3.1:83079230-83079249 MS.gene32754:CDS 30.0%
!!! CTTGTTTTTTGGCAAAATCA+AGG + chr3.1:83079981-83080000 None:intergenic 30.0%
!!! GCATGTACTTACTTGTTTTT+TGG + chr3.1:83079992-83080011 None:intergenic 30.0%
!!! TTGTTTTTTCACCAATTTCC+TGG - chr3.1:83079268-83079287 MS.gene32754:CDS 30.0%
!!! TTTGTCTTCATTTTTTCTGG+TGG + chr3.1:83078979-83078998 None:intergenic 30.0%
AAAACTCCCTCCAAATAACA+GGG - chr3.1:83078428-83078447 MS.gene32754:CDS 35.0%
ACATACGTTATAGCCTGAAT+AGG + chr3.1:83079358-83079377 None:intergenic 35.0%
AGATCATACATGGTAGGATT+CGG - chr3.1:83080362-83080381 MS.gene32754:intron 35.0%
AGTTGGGATCTTAAATATGC+TGG - chr3.1:83079601-83079620 MS.gene32754:CDS 35.0%
CAACAATGATGATCATCAAC+TGG + chr3.1:83078952-83078971 None:intergenic 35.0%
CACTGTGACTACATTATCAT+GGG - chr3.1:83078792-83078811 MS.gene32754:intron 35.0%
CATGTAGGATGAATTGTTGT+GGG - chr3.1:83079489-83079508 MS.gene32754:intron 35.0%
CTAATGTAGCAGAATTCAGT+TGG - chr3.1:83079584-83079603 MS.gene32754:CDS 35.0%
GATTTGGCAAAATCGCATAA+AGG - chr3.1:83079300-83079319 MS.gene32754:intron 35.0%
GCAGGAGATAATGTGAAATA+TGG - chr3.1:83078754-83078773 MS.gene32754:intron 35.0%
GGACAATTGATATCTTCGTT+TGG + chr3.1:83080443-83080462 None:intergenic 35.0%
TACCAGGAGACAAATGTATT+TGG + chr3.1:83079738-83079757 None:intergenic 35.0%
TATGTTAATTCTCTTGCAGC+TGG - chr3.1:83080599-83080618 MS.gene32754:CDS 35.0%
TCACTGTGACTACATTATCA+TGG - chr3.1:83078791-83078810 MS.gene32754:intron 35.0%
TCATGTAGGATGAATTGTTG+TGG - chr3.1:83079488-83079507 MS.gene32754:intron 35.0%
TCGCATAAAGGAACATATGA+TGG - chr3.1:83079312-83079331 MS.gene32754:intron 35.0%
TCTCCTCAACAAAGCAAAAT+TGG - chr3.1:83079119-83079138 MS.gene32754:intron 35.0%
! GACAAATGTATTTGGTGGTA+AGG + chr3.1:83079730-83079749 None:intergenic 35.0%
! GTAATTGATAGTAACACACC+CGG - chr3.1:83079012-83079031 MS.gene32754:CDS 35.0%
! TAGTGAGATTGCTTCTGAAA+CGG - chr3.1:83079035-83079054 MS.gene32754:CDS 35.0%
! TTTTGGATCTTCTATGATGG+CGG + chr3.1:83080232-83080251 None:intergenic 35.0%
!! CAGTTGATGATCATCATTGT+TGG - chr3.1:83078950-83078969 MS.gene32754:CDS 35.0%
ACCATGTATGATCTTCCTTC+TGG + chr3.1:83080356-83080375 None:intergenic 40.0%
AGTGAACAAGAAAGATGTGC+CGG + chr3.1:83079884-83079903 None:intergenic 40.0%
ATATTGGGGAAAAACCCAGA+AGG - chr3.1:83080338-83080357 MS.gene32754:intron 40.0%
ATTGGGCAATCATTGTAGCA+TGG - chr3.1:83078304-83078323 MS.gene32754:CDS 40.0%
CACCAAATACATTTGTCTCC+TGG - chr3.1:83079733-83079752 MS.gene32754:intron 40.0%
CAGGAGACAAATGTATTTGG+TGG + chr3.1:83079735-83079754 None:intergenic 40.0%
CATCATAATATCCCCCAGAT+AGG + chr3.1:83078735-83078754 None:intergenic 40.0%
CCATGTATGATCTTCCTTCT+GGG + chr3.1:83080355-83080374 None:intergenic 40.0%
GAAAACTCCCTCCAAATAAC+AGG - chr3.1:83078427-83078446 MS.gene32754:CDS 40.0%
GAAGGAAGATCATACATGGT+AGG - chr3.1:83080356-83080375 MS.gene32754:intron 40.0%
GATCATACATGGTAGGATTC+GGG - chr3.1:83080363-83080382 MS.gene32754:intron 40.0%
GCCAATACACAATATGCTAC+CGG - chr3.1:83079862-83079881 MS.gene32754:CDS 40.0%
TATGTGCAAGGTTCAGTCTT+AGG + chr3.1:83080581-83080600 None:intergenic 40.0%
TCGAAATGGCTGCAAAGAAA+TGG - chr3.1:83080473-83080492 MS.gene32754:CDS 40.0%
TTCAGAAGCAATCTCACTAC+CGG + chr3.1:83079034-83079053 None:intergenic 40.0%
! AAGGAACCCTGTTATTTGGA+GGG + chr3.1:83078437-83078456 None:intergenic 40.0%
! ATTTGGTGGTAAGGACTATC+AGG + chr3.1:83079721-83079740 None:intergenic 40.0%
! CTAGCAAAGCTTGCTTCTTT+GGG - chr3.1:83080626-83080645 MS.gene32754:CDS 40.0%
! CTCACTCAGCTACATTTTGA+AGG - chr3.1:83079637-83079656 MS.gene32754:intron 40.0%
! TCTAGCAAAGCTTGCTTCTT+TGG - chr3.1:83080625-83080644 MS.gene32754:CDS 40.0%
! TCTTCGTTTGGTGACAACTT+TGG + chr3.1:83080431-83080450 None:intergenic 40.0%
! TGTGAAATATGGGCTACCAA+TGG - chr3.1:83078765-83078784 MS.gene32754:intron 40.0%
! TTCTTAGAGGCACAAAGATC+AGG - chr3.1:83078405-83078424 MS.gene32754:CDS 40.0%
AATAACAGGGTTCCTTGGAG+AGG - chr3.1:83078441-83078460 MS.gene32754:CDS 45.0%
AATGTAGGTCGACCTATCTG+GGG - chr3.1:83078720-83078739 MS.gene32754:intron 45.0%
AATTGTTGTGGGCAGCAACT+TGG - chr3.1:83079500-83079519 MS.gene32754:intron 45.0%
ACCTCTATGATGAGCTTGTG+TGG + chr3.1:83080399-83080418 None:intergenic 45.0%
AGCCATTTCGAAAAGCTCGT+GGG + chr3.1:83080464-83080483 None:intergenic 45.0%
CCAAATCCACATGAGCTAAC+TGG - chr3.1:83080497-83080516 MS.gene32754:CDS 45.0%
CCAGTTAGCTCATGTGGATT+TGG + chr3.1:83080500-83080519 None:intergenic 45.0%
CCCAGAAGGAAGATCATACA+TGG - chr3.1:83080352-83080371 MS.gene32754:intron 45.0%
CCTCTATGATGAGCTTGTGT+GGG + chr3.1:83080398-83080417 None:intergenic 45.0%
CCTGAATAGGAGCAGTAGAA+GGG + chr3.1:83079345-83079364 None:intergenic 45.0%
CTCCAAATAACAGGGTTCCT+TGG - chr3.1:83078436-83078455 MS.gene32754:CDS 45.0%
CTCCAAGGAACCCTGTTATT+TGG + chr3.1:83078441-83078460 None:intergenic 45.0%
CTGAATAGGAGCAGTAGAAG+GGG + chr3.1:83079344-83079363 None:intergenic 45.0%
GAATGTAGGTCGACCTATCT+GGG - chr3.1:83078719-83078738 MS.gene32754:intron 45.0%
GCATGGCTTACTTTGTTCCA+AGG - chr3.1:83078321-83078340 MS.gene32754:CDS 45.0%
GCCGGTAGCATATTGTGTAT+TGG + chr3.1:83079866-83079885 None:intergenic 45.0%
GGACAAGTATCATGCTTCCT+TGG + chr3.1:83078341-83078360 None:intergenic 45.0%
GGCAGCAACTTGGTTATACA+TGG - chr3.1:83079510-83079529 MS.gene32754:intron 45.0%
GTAGTCACAGTGAATGCCAT+TGG + chr3.1:83078784-83078803 None:intergenic 45.0%
TATGTCAGGTCCACCTACAA+TGG + chr3.1:83080525-83080544 None:intergenic 45.0%
TCAGAAGCAATCTCACTACC+GGG + chr3.1:83079033-83079052 None:intergenic 45.0%
TCGAAAAGCTGCCAGGAAAT+TGG + chr3.1:83079282-83079301 None:intergenic 45.0%
TCTGGGGGATATTATGATGC+AGG - chr3.1:83078736-83078755 MS.gene32754:intron 45.0%
TGAATGTAGGTCGACCTATC+TGG - chr3.1:83078718-83078737 MS.gene32754:intron 45.0%
! AGGAAGCATGATACTTGTCC+AGG - chr3.1:83078341-83078360 MS.gene32754:CDS 45.0%
! CAAGGAACCCTGTTATTTGG+AGG + chr3.1:83078438-83078457 None:intergenic 45.0%
! GGAAGCATGATACTTGTCCA+GGG - chr3.1:83078342-83078361 MS.gene32754:CDS 45.0%
! TTCCTGGCAGCTTTTCGATT+TGG - chr3.1:83079284-83079303 MS.gene32754:CDS 45.0%
!! ATACATTAAATTAATAATTT+GGG - chr3.1:83078671-83078690 MS.gene32754:intron 5.0%
!! TAAATTTAATTAAAAAAAAC+TGG + chr3.1:83080148-83080167 None:intergenic 5.0%
ATGGCTCCAGTTAGCTCATG+TGG + chr3.1:83080506-83080525 None:intergenic 50.0%
ATGTAGGTCGACCTATCTGG+GGG - chr3.1:83078721-83078740 MS.gene32754:intron 50.0%
CAGCCATTTCGAAAAGCTCG+TGG + chr3.1:83080465-83080484 None:intergenic 50.0%
CCCACACAAGCTCATCATAG+AGG - chr3.1:83080395-83080414 MS.gene32754:intron 50.0%
CCCTTCTACTGCTCCTATTC+AGG - chr3.1:83079342-83079361 MS.gene32754:CDS 50.0%
CTAACTGGAGCCATTGTAGG+TGG - chr3.1:83080512-83080531 MS.gene32754:CDS 50.0%
CTATGATGAGCTTGTGTGGG+TGG + chr3.1:83080395-83080414 None:intergenic 50.0%
GAGCTAACTGGAGCCATTGT+AGG - chr3.1:83080509-83080528 MS.gene32754:CDS 50.0%
GCCTGAATAGGAGCAGTAGA+AGG + chr3.1:83079346-83079365 None:intergenic 50.0%
GGTGGTAAGGACTATCAGGA+AGG + chr3.1:83079717-83079736 None:intergenic 50.0%
TGCCAAATCGAAAAGCTGCC+AGG + chr3.1:83079289-83079308 None:intergenic 50.0%
! GTCCCACGAGCTTTTCGAAA+TGG - chr3.1:83080459-83080478 MS.gene32754:CDS 50.0%
!! TTCTGAAACGGCTGCTGCTA+TGG - chr3.1:83079047-83079066 MS.gene32754:CDS 50.0%
GGTTCCTTGGAGAGGAGACT+CGG - chr3.1:83078449-83078468 MS.gene32754:CDS 55.0%
ACTGCCGAGTCTCCTCTCCA+AGG + chr3.1:83078456-83078475 None:intergenic 60.0%
GGAGACTCGGCAGTCGATGA+TGG - chr3.1:83078462-83078481 MS.gene32754:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 83078285 83080652 83078285 ID=MS.gene32754
chr3.1 mRNA 83078285 83080652 83078285 ID=MS.gene32754.t1;Parent=MS.gene32754
chr3.1 exon 83080440 83080652 83080440 ID=MS.gene32754.t1.exon1;Parent=MS.gene32754.t1
chr3.1 CDS 83080440 83080652 83080440 ID=cds.MS.gene32754.t1;Parent=MS.gene32754.t1
chr3.1 exon 83080027 83080210 83080027 ID=MS.gene32754.t1.exon2;Parent=MS.gene32754.t1
chr3.1 CDS 83080027 83080210 83080027 ID=cds.MS.gene32754.t1;Parent=MS.gene32754.t1
chr3.1 exon 83079797 83079993 83079797 ID=MS.gene32754.t1.exon3;Parent=MS.gene32754.t1
chr3.1 CDS 83079797 83079993 83079797 ID=cds.MS.gene32754.t1;Parent=MS.gene32754.t1
chr3.1 exon 83079566 83079643 83079566 ID=MS.gene32754.t1.exon4;Parent=MS.gene32754.t1
chr3.1 CDS 83079566 83079643 83079566 ID=cds.MS.gene32754.t1;Parent=MS.gene32754.t1
chr3.1 exon 83079336 83079441 83079336 ID=MS.gene32754.t1.exon5;Parent=MS.gene32754.t1
chr3.1 CDS 83079336 83079441 83079336 ID=cds.MS.gene32754.t1;Parent=MS.gene32754.t1
chr3.1 exon 83079183 83079305 83079183 ID=MS.gene32754.t1.exon6;Parent=MS.gene32754.t1
chr3.1 CDS 83079183 83079305 83079183 ID=cds.MS.gene32754.t1;Parent=MS.gene32754.t1
chr3.1 exon 83078938 83079101 83078938 ID=MS.gene32754.t1.exon7;Parent=MS.gene32754.t1
chr3.1 CDS 83078938 83079101 83078938 ID=cds.MS.gene32754.t1;Parent=MS.gene32754.t1
chr3.1 exon 83078285 83078608 83078285 ID=MS.gene32754.t1.exon8;Parent=MS.gene32754.t1
chr3.1 CDS 83078285 83078608 83078285 ID=cds.MS.gene32754.t1;Parent=MS.gene32754.t1
Gene Sequence

>MS.gene32754

ATGGATTCTAAAAGAGTATATTGGGCAATCATTGTAGCATGGCTTACTTTGTTCCAAGGAAGCATGATACTTGTCCAGGGTCAATTTAATTATAAAGAGGCTCTCACAAAATCCCTTATATTCTTAGAGGCACAAAGATCAGGAAAACTCCCTCCAAATAACAGGGTTCCTTGGAGAGGAGACTCGGCAGTCGATGATGGAAAACTCGCTAATGTATCTTTCTTTTTCTCATAAAATGAAATTATCACTACTATAGAACATTGAATTAACGACAAAAAAGTGTACAGAAAAAGAAAAAAACATGAAATACGCCTCTAAATGTCAGAAATTCCAAAATTGATGACCAAATTCATTATTTTCCGTCTAACATATTTTAATCATGAAAGATACATTAAATTAATAATTTGGGCTAATGTGCATAATTTTATTGAATTGAATGTAGGTCGACCTATCTGGGGGATATTATGATGCAGGAGATAATGTGAAATATGGGCTACCAATGGCATTCACTGTGACTACATTATCATGGGCTGCAATATTTTACAAAACAGAATTTGAAGCAACAAAGGAAATGGGAAATATTCAAGATGCAATCAAATGGGGAACAGATTATTTTCTAAAAGCTAGTTCTAGAAGAAACAAATTATATGTTGAGGTAGGGGACCCAGTTGATGATCATCATTGTTGGGCTCCACCAGAAAAAATGAAGACAAAAAGATCCGTTAAAGTAATTGATAGTAACACACCCGGTAGTGAGATTGCTTCTGAAACGGCTGCTGCTATGGCTTCTTCTTCCATTGTTTTAAGACATACTGATCGTAAATATGCTCGTCGTCTCCTCAACAAAGCAAAATTGGTATGGAATATATGTAGTCCTTTGCTTATATTTAAATTCAGTTGAAGTATTTGTTACATGCATAGGAGATGTTTTTTTCATGAAAATAGCCAATAGTATTTTGGTTACATGGGATTATGAAAACTAATTGTTTTTTCACCAATTTCCTGGCAGCTTTTCGATTTGGCAAAATCGCATAAAGGAACATATGATGGAGAGTGCCCCTTCTACTGCTCCTATTCAGGCTATAACGTATGTTATTACTCTTATAAACAACATTTCCTTATTTACTAAGCTTTGATAATTAGTGTTATATGAGACTGAATTAATGTTGGGATTGTAATAATTGATTGTACATATCTTTGGAATCATGTAGGATGAATTGTTGTGGGCAGCAACTTGGTTATACATGGCAACAAAGAAATCGATGTACATGAAATATATACAAGAAGAATCAATTAGTGCTAATGTAGCAGAATTCAGTTGGGATCTTAAATATGCTGGAGTTCAAGTTCTTCTCACTCAGCTACATTTTGAAGGTCAAAAAGGTCTTGAAACATTTAAAGCACATGGTGAAAGTTACATATGCTCAGTCCTTCCTGATAGTCCTTACCACCAAATACATTTGTCTCCTGGTAAATATATATACGTTTATGATTATATTTAATTGTCATCTTTACATGTATAATCTATAATTATATATGATGATTATGCAGGTGGATTTATTCATATGAGAGATGGAGCCAATACACAATATGCTACCGGCACATCTTTCTTGTTCACTGTATACAGTGATTTGCTTGCCAAATATAAACAAAAAGTTAAATGTGGAGACAAAGAATTTGACTCATCTCATATCCTTGATTTTGCCAAAAAACAAGTAAGTACATGCACCATTTATGCTCACATATTAATTTTATATTATCAAACAAATTTGGAGGCTCACAAAACGAAGTTAATCTCAAATTTTGCAATCTCTAATAATATTCAACGACGAAATTCAAGACATACTTCTATTTTTGTTTCCAGTTTTTTTTAATTAAATTTAGAGAAGCTTTTCTCTCTAATTTCTTTAAATCTAAAGTCTTTATTACTGCGGGAGCACAATACCGCCATCATAGAAGATCCAAAATTGATATAGTGTATACTTTAGAAATAAACAATATAGACATCTATAGCGTGAACATATAATATTTGGTTTGTTAACAGATGGATTACATATTGGGGAAAAACCCAGAAGGAAGATCATACATGGTAGGATTCGGGAAAAATCCACCCACACAAGCTCATCATAGAGGTGCTTCAGTGCCAAAGTTGTCACCAAACGAAGATATCAATTGTCCCACGAGCTTTTCGAAATGGCTGCAAAGAAATGGACCAAATCCACATGAGCTAACTGGAGCCATTGTAGGTGGACCTGACATAAATGACAAATTCGATGATAATCGTACAGATTCACCTAAGACTGAACCTTGCACATATGTTAATTCTCTTGCAGCTGGTGCTCTAGCAAAGCTTGCTTCTTTGGGTTAA
Protein sequence

>MS.gene32754.t1

MDSKRVYWAIIVAWLTLFQGSMILVQGQFNYKEALTKSLIFLEAQRSGKLPPNNRVPWRGDSAVDDGKLANVDLSGGYYDAGDNVKYGLPMAFTVTTLSWAAIFYKTEFEATKEMGNIQDAIKWGTDYFLKARDPVDDHHCWAPPEKMKTKRSVKVIDSNTPGSEIASETAAAMASSSIVLRHTDRKYARRLLNKAKLLFDLAKSHKGTYDGECPFYCSYSGYNDELLWAATWLYMATKKSMYMKYIQEESISANVAEFILLTQLHFEGQKGLETFKAHGESYICSVLPDSPYHQIHLSPGGFIHMRDGANTQYATGTSFLFTVYSDLLAKYKQKVKCGDKEFDSSHILDFAKKQMDYILGKNPEGRSYMVGFGKNPPTQAHHRGASVPKLSPNEDINCPTSFSKWLQRNGPNPHELTGAIVGGPDINDKFDDNRTDSPKTEPCTYVNSLAAGALAKLASLG