AlfalfaGEDB | MS.gene72272

M. sativa cultivar XinJiangDaYe / MS.gene72272

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005975 GO:0008150 GO:0008152 GO:0016787 GO:0016798 GO:0044238 GO:0071704
PFAM: PF00759.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72272.t1	XP_003601390.1	98.7	525	7	0	1	525	1	525	0.00E+00	1085.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72272.t1	O48766	73.0	514	128	2	22	525	11	523	1.9e-233	809.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72272.t1	G7J6Q9	98.7	525	7	0	1	525	1	525	0.0e+00	1085.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene72272	0.810386	1.18E-50	-1.69E-46
MS.gene049887	MS.gene72272	0.835046	2.11E-56	-1.69E-46
MS.gene050807	MS.gene72272	0.829471	5.05E-55	-1.69E-46
MS.gene050808	MS.gene72272	0.805952	1.04E-49	-1.69E-46
MS.gene050959	MS.gene72272	0.800396	1.47E-48	-1.69E-46
MS.gene051102	MS.gene72272	0.830498	2.84E-55	-1.69E-46
MS.gene051719	MS.gene72272	0.809442	1.89E-50	-1.69E-46
MS.gene051720	MS.gene72272	0.813808	2.12E-51	-1.69E-46
MS.gene052133	MS.gene72272	0.805476	1.31E-49	-1.69E-46
MS.gene052397	MS.gene72272	0.809616	1.73E-50	-1.69E-46
MS.gene052516	MS.gene72272	0.806642	7.45E-50	-1.69E-46
MS.gene052665	MS.gene72272	0.825369	4.87E-54	-1.69E-46
MS.gene052732	MS.gene72272	0.836999	6.73E-57	-1.69E-46
MS.gene053144	MS.gene72272	0.843432	1.41E-58	-1.69E-46
MS.gene053174	MS.gene72272	0.809085	2.25E-50	-1.69E-46
MS.gene053581	MS.gene72272	0.856105	4.03E-62	-1.69E-46
MS.gene054550	MS.gene72272	0.817121	3.89E-52	-1.69E-46
MS.gene054584	MS.gene72272	0.833738	4.49E-56	-1.69E-46
MS.gene055465	MS.gene72272	0.834077	3.69E-56	-1.69E-46
MS.gene055466	MS.gene72272	0.817742	2.82E-52	-1.69E-46
MS.gene055468	MS.gene72272	0.821261	4.44E-53	-1.69E-46
MS.gene055469	MS.gene72272	0.843174	1.65E-58	-1.69E-46
MS.gene055537	MS.gene72272	0.814373	1.59E-51	-1.69E-46
MS.gene055891	MS.gene72272	0.817485	3.22E-52	-1.69E-46
MS.gene057057	MS.gene72272	0.80015	1.65E-48	-1.69E-46
MS.gene058852	MS.gene72272	0.825265	5.15E-54	-1.69E-46
MS.gene058945	MS.gene72272	0.809155	2.18E-50	-1.69E-46
MS.gene059171	MS.gene72272	0.829477	5.04E-55	-1.69E-46
MS.gene059713	MS.gene72272	0.827146	1.84E-54	-1.69E-46
MS.gene059937	MS.gene72272	0.813239	2.83E-51	-1.69E-46
MS.gene060884	MS.gene72272	0.804858	1.77E-49	-1.69E-46
MS.gene061006	MS.gene72272	0.840531	8.22E-58	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene72272	MS.gene51667	PPI
MS.gene68175	MS.gene72272	PPI
MS.gene56303	MS.gene72272	PPI
MS.gene014436	MS.gene72272	PPI
MS.gene72272	MS.gene019323	PPI
MS.gene72272	MS.gene026233	PPI
MS.gene72272	MS.gene50877	PPI
MS.gene020485	MS.gene72272	PPI
MS.gene72272	MS.gene21633	PPI
MS.gene72272	MS.gene20757	PPI
MS.gene72272	MS.gene96428	PPI
MS.gene72272	MS.gene026232	PPI
MS.gene72272	MS.gene81916	PPI
MS.gene72272	MS.gene014234	PPI
MS.gene72272	MS.gene95200	PPI
MS.gene72272	MS.gene063734	PPI
MS.gene072765	MS.gene72272	PPI
MS.gene009506	MS.gene72272	PPI
MS.gene72272	MS.gene020482	PPI
MS.gene72272	MS.gene68770	PPI
MS.gene72272	MS.gene42640	PPI
MS.gene072763	MS.gene72272	PPI
MS.gene72272	MS.gene024650	PPI
MS.gene72272	MS.gene064746	PPI
MS.gene024647	MS.gene72272	PPI
MS.gene51960	MS.gene72272	PPI
MS.gene72272	MS.gene42272	PPI
MS.gene014960	MS.gene72272	PPI
MS.gene72272	MS.gene057924	PPI
MS.gene72272	MS.gene21635	PPI
MS.gene72272	MS.gene063122	PPI
MS.gene72272	MS.gene22342	PPI
MS.gene72272	MS.gene88560	PPI
MS.gene72272	MS.gene51810	PPI
MS.gene020483	MS.gene72272	PPI
MS.gene72272	MS.gene02701	PPI
MS.gene72272	MS.gene69412	PPI
MS.gene72272	MS.gene015691	PPI
MS.gene72272	MS.gene057923	PPI
MS.gene72272	MS.gene88559	PPI
MS.gene72272	MS.gene95918	PPI
MS.gene72272	MS.gene32754	PPI
MS.gene72272	MS.gene81918	PPI
MS.gene72272	MS.gene07235	PPI
MS.gene29940	MS.gene72272	PPI
MS.gene72272	MS.gene95919	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72272.t1	MTR_3g080190	98.667	525	7	0	1	525	1	525	0.0	1090
MS.gene72272.t1	MTR_5g085730	82.016	506	89	2	21	525	12	516	0.0	886
MS.gene72272.t1	MTR_5g093590	71.546	485	137	1	22	505	16	500	0.0	745
MS.gene72272.t1	MTR_8g099410	61.803	466	176	1	40	505	28	491	0.0	630
MS.gene72272.t1	MTR_8g099410	61.803	466	176	1	40	505	28	491	0.0	627
MS.gene72272.t1	MTR_4g074960	60.897	468	180	3	39	505	27	492	0.0	599
MS.gene72272.t1	MTR_1g037340	50.826	484	226	6	23	503	5	479	1.98e-169	488
MS.gene72272.t1	MTR_5g009970	48.408	471	224	6	39	506	40	494	2.06e-154	450
MS.gene72272.t1	MTR_5g010000	48.745	478	226	6	36	510	36	497	3.41e-154	450
MS.gene72272.t1	MTR_8g104820	49.362	470	225	8	40	505	30	490	6.23e-153	446
MS.gene72272.t1	MTR_3g080810	48.932	468	228	7	40	505	35	493	7.52e-153	446
MS.gene72272.t1	MTR_3g117500	48.723	470	230	6	40	506	24	485	7.64e-152	443
MS.gene72272.t1	MTR_3g105230	46.436	463	232	5	31	493	21	467	6.92e-147	431
MS.gene72272.t1	MTR_4g035930	44.491	481	252	6	26	505	1	467	3.14e-145	426
MS.gene72272.t1	MTR_4g035955	45.322	481	247	7	26	505	1	466	5.59e-145	425
MS.gene72272.t1	MTR_3g023740	45.657	495	245	6	23	509	5	483	8.10e-143	420
MS.gene72272.t1	MTR_3g010330	47.122	469	232	7	40	505	25	480	2.69e-140	414
MS.gene72272.t1	MTR_4g035975	45.875	497	239	10	25	505	7	489	2.22e-138	409
MS.gene72272.t1	MTR_1g088190	46.341	492	220	10	15	503	26	476	4.21e-138	408
MS.gene72272.t1	MTR_5g009970	47.430	428	206	6	82	506	9	420	6.40e-134	395
MS.gene72272.t1	MTR_3g113720	43.312	471	253	9	41	506	58	519	4.43e-125	376
MS.gene72272.t1	MTR_5g009970	45.721	409	203	6	101	506	1	393	1.56e-121	363
MS.gene72272.t1	MTR_8g075990	43.220	472	252	10	41	506	64	525	7.14e-120	363
MS.gene72272.t1	MTR_2g028480	42.270	511	252	16	40	523	111	605	2.67e-104	326
MS.gene72272.t1	MTR_3g082440	39.095	486	260	15	40	502	116	588	1.20e-93	298
MS.gene72272.t1	MTR_3g090540	36.296	540	277	18	19	510	68	588	3.43e-93	296
MS.gene72272.t1	MTR_5g009970	45.161	341	168	6	169	506	1	325	1.07e-88	276
MS.gene72272.t1	MTR_1g088190	44.776	335	144	8	15	347	26	321	7.96e-76	243
MS.gene72272.t1	MTR_1g037320	54.658	161	72	1	101	261	1	160	1.91e-46	164
MS.gene72272.t1	MTR_1g037320	48.361	122	62	1	382	503	169	289	1.51e-33	129
MS.gene72272.t1	MTR_7g108925	47.742	155	80	1	349	503	43	196	4.70e-46	161
MS.gene72272.t1	MTR_0393s0050	45.714	140	76	0	349	488	20	159	8.68e-38	137
MS.gene72272.t1	MTR_4g128810	27.812	320	154	10	216	502	55	330	2.64e-28	116
MS.gene72272.t1	MTR_4g047570	33.043	115	54	3	289	402	2	94	1.37e-14	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72272.t1	AT2G32990	75.051	493	113	1	22	504	11	503	0.0	788
MS.gene72272.t1	AT1G64390	62.446	466	173	1	40	505	25	488	0.0	624
MS.gene72272.t1	AT4G11050	61.456	467	178	1	40	506	26	490	0.0	613
MS.gene72272.t1	AT4G11050	61.456	467	178	1	40	506	26	490	0.0	613
MS.gene72272.t1	AT4G11050	61.456	467	178	1	40	506	26	490	0.0	611
MS.gene72272.t1	AT1G48930	58.584	466	191	2	39	504	29	492	0.0	551
MS.gene72272.t1	AT2G44550	51.566	479	223	4	25	503	18	487	8.75e-169	486
MS.gene72272.t1	AT2G44540	50.830	482	227	5	22	503	17	488	2.51e-164	475
MS.gene72272.t1	AT2G44560	51.458	480	224	5	24	503	18	488	3.04e-162	470
MS.gene72272.t1	AT2G44570	48.447	483	240	5	24	505	17	491	1.43e-160	466
MS.gene72272.t1	AT1G70710	47.826	483	242	6	24	505	12	485	2.23e-152	445
MS.gene72272.t1	AT1G02800	50.968	465	216	6	40	503	43	496	3.27e-152	445
MS.gene72272.t1	AT4G02290	50.000	468	222	7	40	505	52	509	3.62e-152	445
MS.gene72272.t1	AT4G39010	47.234	470	236	7	40	505	29	490	5.80e-148	434
MS.gene72272.t1	AT1G23210	47.966	467	233	6	40	505	28	485	1.71e-147	432
MS.gene72272.t1	AT4G09740	47.844	487	232	8	24	506	7	475	4.63e-147	431
MS.gene72272.t1	AT4G23560	47.737	486	234	8	24	506	7	475	5.17e-147	431
MS.gene72272.t1	AT3G43860	45.530	481	245	6	26	505	19	483	1.96e-142	419
MS.gene72272.t1	AT3G43860	45.530	481	245	6	26	505	19	483	1.96e-142	419
MS.gene72272.t1	AT1G22880	46.901	484	242	8	24	505	8	478	3.79e-141	416
MS.gene72272.t1	AT1G71380	46.201	487	244	10	24	505	5	478	3.41e-139	411
MS.gene72272.t1	AT1G19940	47.619	462	232	7	40	500	48	500	2.33e-138	410
MS.gene72272.t1	AT1G19940	47.619	462	232	7	40	500	48	500	2.33e-138	410
MS.gene72272.t1	AT4G39000	45.895	475	245	7	40	510	25	491	8.22e-137	405
MS.gene72272.t1	AT1G75680	47.702	457	229	7	41	496	58	505	1.02e-133	398
MS.gene72272.t1	AT4G38990	43.673	490	259	9	25	505	8	489	1.15e-127	382
MS.gene72272.t1	AT4G02290	47.420	407	202	7	101	505	1	397	7.43e-119	356
MS.gene72272.t1	AT2G44570	45.823	395	205	5	24	417	17	403	1.72e-115	347
MS.gene72272.t1	AT1G22880	45.209	407	208	8	101	505	1	394	3.76e-113	341
MS.gene72272.t1	AT4G39010	46.448	366	185	6	40	401	29	387	3.76e-110	333
MS.gene72272.t1	AT1G19940	45.879	364	192	5	40	402	48	407	1.00e-99	307
MS.gene72272.t1	AT5G49720	42.449	490	241	16	40	502	108	583	2.36e-97	308
MS.gene72272.t1	AT4G24260	41.104	489	251	15	40	503	108	584	1.12e-87	282
MS.gene72272.t1	AT1G65610	35.887	496	283	15	40	513	116	598	1.95e-87	282
MS.gene72272.t1	AT4G38990	43.017	358	174	6	25	381	8	336	1.52e-83	263
MS.gene72272.t1	AT1G22880	48.476	328	155	7	24	349	8	323	5.44e-83	261
MS.gene72272.t1	AT4G24260	39.433	388	203	13	40	402	108	488	1.07e-55	194
MS.gene72272.t1	AT4G24260	41.390	331	162	11	40	342	108	434	1.10e-53	188

Find 114 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCACAAGTTGATTACATTT+TGG	0.156175	3.1:-68095504	MS.gene72272:CDS
AGAACCAAATCCTAATATTT+TGG	0.167708	3.1:-68095330	MS.gene72272:CDS
CATACAACCAACGTGTTAAT+TGG	0.167826	3.1:-68098974	MS.gene72272:CDS
AAATATTAGGATTTGGTTCT+TGG	0.200576	3.1:+68095333	None:intergenic
ATGTAATGGAAGCAATTAAA+TGG	0.203237	3.1:-68098668	MS.gene72272:CDS
AACTCATGTTAAGTGGATTT+TGG	0.205279	3.1:+68095480	None:intergenic
ACCTCTATGATGCACCCTTT+TGG	0.242199	3.1:+68095430	None:intergenic
ATGAGTTACTTAGTGGGATA+TGG	0.273448	3.1:-68095464	MS.gene72272:CDS
GCAGGTGATCATGTGAAATT+TGG	0.274678	3.1:-68098777	MS.gene72272:CDS
TACTTTGAAGCACAACGTTC+TGG	0.276345	3.1:-68099005	MS.gene72272:CDS
ATTGTGCAGTTATTTGAGTT+TGG	0.280930	3.1:-68097384	MS.gene72272:intron
TTGTGCAGTTATTTGAGTTT+GGG	0.294397	3.1:-68097383	MS.gene72272:intron
ACCTCTGCCCACAACACATT+TGG	0.300565	3.1:+68098612	None:intergenic
CCAACCAAAATATTAGGATT+TGG	0.302553	3.1:+68095326	None:intergenic
ATATATAGAGAGTATTTAAA+AGG	0.306750	3.1:+68095130	None:intergenic
GTTAATTGGCGTCATCATTC+TGG	0.309546	3.1:-68098960	MS.gene72272:CDS
AAAGCTCCAACCAAAATATT+AGG	0.310755	3.1:+68095320	None:intergenic
CAGCTGCTTCCATTGTGTTT+AGG	0.320538	3.1:-68098132	MS.gene72272:CDS
TCAAAATTGTCTAGATTATC+AGG	0.328331	3.1:+68095287	None:intergenic
AGAGAGAATAAAGGGTTCAT+AGG	0.328810	3.1:-68095389	MS.gene72272:CDS
ATTTCTAATGCTCATACATT+TGG	0.331259	3.1:-68097216	MS.gene72272:CDS
GCACACAAGGGTATGATAAT+TGG	0.347724	3.1:-68095364	MS.gene72272:CDS
CAATTAACACGTTGGTTGTA+TGG	0.354636	3.1:+68098975	None:intergenic
CATGGAGTGCTATTGAGTTT+AGG	0.363527	3.1:-68098722	MS.gene72272:CDS
GTGACAATGATGTTTAAATC+TGG	0.367337	3.1:+68099109	None:intergenic
GCTCATACATTTGGTGGTAT+TGG	0.372035	3.1:-68097207	MS.gene72272:CDS
TTTCAGGTGGATTTGGTTGG+AGG	0.401221	3.1:-68098810	MS.gene72272:intron
TGTAATGGAAGCAATTAAAT+GGG	0.406228	3.1:-68098667	MS.gene72272:CDS
CCTCTGCCCACAACACATTT+GGG	0.407042	3.1:+68098613	None:intergenic
CAAAAGATCAATTGCCAAGA+TGG	0.412945	3.1:-68095563	MS.gene72272:CDS
GTATCATATAGAGAGAATAA+AGG	0.414088	3.1:-68095398	MS.gene72272:CDS
CTGGACGTTGCCAACAATAA+TGG	0.415828	3.1:+68098242	None:intergenic
CTTGAGGTTGTCATGTCCTC+TGG	0.417866	3.1:+68098223	None:intergenic
GTCAAAATATTCTTGTTTGT+CGG	0.429086	3.1:+68097245	None:intergenic
AAGATAGATGAGAAGAATCC+AGG	0.433832	3.1:-68098190	MS.gene72272:CDS
TTCAGGTGGATTTGGTTGGA+GGG	0.437613	3.1:-68098809	MS.gene72272:intron
TCATGCAAAGAGGGAATAGA+AGG	0.439518	3.1:-68095187	MS.gene72272:CDS
ATCCCAGCTGAATTCTGTTA+TGG	0.440268	3.1:+68097179	None:intergenic
ACTTTGAAGCACAACGTTCT+GGG	0.441438	3.1:-68099004	MS.gene72272:CDS
GGGAAATATGATGGAAGTGT+TGG	0.451425	3.1:-68097348	MS.gene72272:CDS
TATCATATAGAGAGAATAAA+GGG	0.456692	3.1:-68095397	MS.gene72272:CDS
TCTAATGCTCATACATTTGG+TGG	0.457457	3.1:-68097213	MS.gene72272:CDS
AGCTGGGATGTTAAGTATGC+TGG	0.465855	3.1:-68097165	MS.gene72272:CDS
CAAAATTGAGAATTTCATCA+TGG	0.467727	3.1:+68095531	None:intergenic
ATGGGCCTAAGTACCCCAAA+AGG	0.469761	3.1:-68095445	MS.gene72272:CDS
ATACATTTGGTGGTATTGGT+TGG	0.472675	3.1:-68097203	MS.gene72272:CDS
TACTAGCCCAAATGTGTTGT+GGG	0.473525	3.1:-68098619	MS.gene72272:CDS
AGGCAAGAAATTGTTGAAGC+TGG	0.479140	3.1:-68098702	MS.gene72272:CDS
CCCAAATGTGTTGTGGGCAG+AGG	0.481112	3.1:-68098613	MS.gene72272:intron
ACTAAGTAACTCATGTTAAG+TGG	0.486492	3.1:+68095473	None:intergenic
GGTCCATAACAGAATTCAGC+TGG	0.486609	3.1:-68097182	MS.gene72272:CDS
ACTTAACATGAGTTACTTAG+TGG	0.491572	3.1:-68095471	MS.gene72272:CDS
TGAGTTACTTAGTGGGATAT+GGG	0.492298	3.1:-68095463	MS.gene72272:CDS
TTGGTTGGAGCTTTGGTAGG+AGG	0.493848	3.1:-68095311	MS.gene72272:CDS
TGGAGAAACTGCTGCAGCTA+TGG	0.496286	3.1:-68098155	MS.gene72272:CDS
CATCATTCTGGTCTTACAGA+TGG	0.500607	3.1:-68098948	MS.gene72272:CDS
GAAGAATCCAGGTTCAGATC+TGG	0.506230	3.1:-68098179	MS.gene72272:CDS
ATTGTTGAAGCTGGTGAACT+TGG	0.513050	3.1:-68098693	MS.gene72272:CDS
TTACCTTTGATGCTAAAACT+TGG	0.515672	3.1:+68097139	None:intergenic
CTTACAGATGGTTTAGAACA+AGG	0.516101	3.1:-68098936	MS.gene72272:CDS
ATAATCAAAAGATTGTGTTA+TGG	0.516983	3.1:+68099055	None:intergenic
TGTGAAATTTGGTTTACCAA+TGG	0.517414	3.1:-68098766	MS.gene72272:CDS
CATGGCTCACAATCCCATCT+TGG	0.518926	3.1:+68095549	None:intergenic
AGCTACTATGCGTCGGTGAG+TGG	0.518978	3.1:-68097315	MS.gene72272:CDS
GAGAGAATAAAGGGTTCATA+GGG	0.518983	3.1:-68095388	MS.gene72272:CDS
TTGCAAAATTCATGCAAAGA+GGG	0.519154	3.1:-68095196	MS.gene72272:CDS
AAAAGATCAATTGCCAAGAT+GGG	0.520703	3.1:-68095562	MS.gene72272:CDS
ATATAATACTGCACCATTAG+TGG	0.523912	3.1:-68095225	MS.gene72272:CDS
AAATACAGAGGGAAATATGA+TGG	0.525154	3.1:-68097357	MS.gene72272:CDS
GAGACACAGACCATTATTGT+TGG	0.526224	3.1:-68098252	MS.gene72272:CDS
CAAGGGTATGATAATTGGTA+TGG	0.527762	3.1:-68095359	MS.gene72272:CDS
TGTGCAGTTATTTGAGTTTG+GGG	0.529149	3.1:-68097382	MS.gene72272:intron
AAAATATTCTTGTTTGTCGG+TGG	0.547238	3.1:+68097248	None:intergenic
TTTGCAAAATTCATGCAAAG+AGG	0.550554	3.1:-68095197	MS.gene72272:CDS
GATACATGGATGAGTTATTG+TGG	0.552659	3.1:-68097293	MS.gene72272:CDS
TATAATACTGCACCATTAGT+GGG	0.560164	3.1:-68095224	MS.gene72272:CDS
GTTGGAGGGTACTATGATGC+AGG	0.563279	3.1:-68098795	MS.gene72272:CDS
TGACAATGATGTTTAAATCT+GGG	0.565379	3.1:+68099110	None:intergenic
TGCGTCGGTGAGTGGATACA+TGG	0.568122	3.1:-68097307	MS.gene72272:CDS
AATGTAATCAACTTGTGACT+TGG	0.573884	3.1:+68095508	None:intergenic
GGGTTCATAGGGTGCACACA+AGG	0.574402	3.1:-68095377	MS.gene72272:CDS
ATACTAGCCCAAATGTGTTG+TGG	0.577675	3.1:-68098620	MS.gene72272:CDS
TCTCCAGCCAGATCTGAACC+TGG	0.578429	3.1:+68098172	None:intergenic
ATACATGGATGAGTTATTGT+GGG	0.579757	3.1:-68097292	MS.gene72272:CDS
TACAGATGGTTTAGAACAAG+GGG	0.583836	3.1:-68098934	MS.gene72272:intron
GCAAAGAGGGAATAGAAGGT+TGG	0.590189	3.1:-68095183	MS.gene72272:CDS
TATCTTAAAAGCTTGTCTTG+AGG	0.590517	3.1:+68098207	None:intergenic
AGTTATTGTGGGGAGCTATG+TGG	0.591433	3.1:-68097281	MS.gene72272:CDS
AATCCAGGTTCAGATCTGGC+TGG	0.593866	3.1:-68098175	MS.gene72272:CDS
CTTAACATGAGTTACTTAGT+GGG	0.597597	3.1:-68095470	MS.gene72272:CDS
ATTCTCTCTATATGATACAA+TGG	0.597953	3.1:+68095403	None:intergenic
TGGTGAACTTGGTCATGTAA+TGG	0.602665	3.1:-68098682	MS.gene72272:CDS
TTACTGTCACAATGTTGTCA+TGG	0.603705	3.1:-68098740	MS.gene72272:CDS
TGGGCCTAAGTACCCCAAAA+GGG	0.608177	3.1:-68095444	MS.gene72272:CDS
CTTACATAGAGTAATCCAGC+TGG	0.611840	3.1:+68095665	None:intergenic
TTACAGATGGTTTAGAACAA+GGG	0.612255	3.1:-68098935	MS.gene72272:CDS
AATGTGGAAAGAACACCAGC+TGG	0.616199	3.1:-68095680	MS.gene72272:CDS
CCCAAAAGGGTGCATCATAG+AGG	0.625621	3.1:-68095431	MS.gene72272:CDS
AGTTTGGGGACAAATACAGA+GGG	0.630720	3.1:-68097368	MS.gene72272:CDS
ATTGTGACAGTAAACGCCAT+TGG	0.633379	3.1:+68098750	None:intergenic
TTACTACTACCTGCTGAGCA+TGG	0.641231	3.1:+68098074	None:intergenic
GTCCATAACAGAATTCAGCT+GGG	0.643895	3.1:-68097181	MS.gene72272:CDS
GATGACGCCAATTAACACGT+TGG	0.651812	3.1:+68098967	None:intergenic
TTATTGTTGGCAACGTCCAG+AGG	0.652974	3.1:-68098239	MS.gene72272:CDS
AGTGAAGAGCTACTATGCGT+CGG	0.655502	3.1:-68097322	MS.gene72272:CDS
AAAAGTAGCTGAGAATAATG+TGG	0.661774	3.1:+68098100	None:intergenic
GAGTTTGGGGACAAATACAG+AGG	0.667296	3.1:-68097369	MS.gene72272:CDS
GAACAATTATGTGCAAACTG+AGG	0.668349	3.1:-68095255	MS.gene72272:CDS
GTAATGGAAGCAATTAAATG+GGG	0.669304	3.1:-68098666	MS.gene72272:CDS
AAACAATGGCAGCAGCAATG+TGG	0.670730	3.1:-68095696	MS.gene72272:CDS
GGTTCATAGGGTGCACACAA+GGG	0.675227	3.1:-68095376	MS.gene72272:CDS
TTGAAGCACAACGTTCTGGG+AGG	0.691689	3.1:-68099001	MS.gene72272:CDS
GATTACTCTATGTAAGACAG+TGG	0.704386	3.1:-68095658	MS.gene72272:CDS
TACATGGATGAGTTATTGTG+GGG	0.766782	3.1:-68097291	MS.gene72272:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATGAATAATTTATTATGTT+TGG	-	chr3.1:68096313-68096332	MS.gene72272:intron	10.0%
!!!	TACTAATATTTTTACTTAAT+TGG	+	chr3.1:68096815-68096834	None:intergenic	10.0%
!!	AAAATACAAACAAAACAAAA+GGG	-	chr3.1:68097182-68097201	MS.gene72272:CDS	15.0%
!!	TAAAATACAAACAAAACAAA+AGG	-	chr3.1:68097181-68097200	MS.gene72272:CDS	15.0%
!!	TATTAAAGTATGTAAAACAA+GGG	-	chr3.1:68096391-68096410	MS.gene72272:intron	15.0%
!!!	AAAGTTGAGATTTTTATTTT+TGG	-	chr3.1:68095457-68095476	MS.gene72272:CDS	15.0%
!!!	AAGTTGAGATTTTTATTTTT+GGG	-	chr3.1:68095458-68095477	MS.gene72272:CDS	15.0%
!!!	TATCATTTTATTTGATTTGA+TGG	-	chr3.1:68097486-68097505	MS.gene72272:intron	15.0%
!!!	TTTTGATTGTGTTTTTTTTT+TGG	-	chr3.1:68096496-68096515	MS.gene72272:intron	15.0%
!!	AAAAAATAACAAATGGATCA+AGG	+	chr3.1:68098227-68098246	None:intergenic	20.0%
!!	AAAAATAACAAATGGATCAA+GGG	+	chr3.1:68098226-68098245	None:intergenic	20.0%
!!	AAAAGAACAACAAATATTTC+TGG	-	chr3.1:68098341-68098360	MS.gene72272:intron	20.0%
!!	ACTATCCAAAAAATAACAAA+TGG	+	chr3.1:68098234-68098253	None:intergenic	20.0%
!!	ATAATCAAAAGATTGTGTTA+TGG	+	chr3.1:68095248-68095267	None:intergenic	20.0%
!!	GAAGTCAAAAATAAAATGAT+GGG	-	chr3.1:68097413-68097432	MS.gene72272:intron	20.0%
!!	GACTATATAGCTAATATAAA+TGG	-	chr3.1:68097682-68097701	MS.gene72272:intron	20.0%
!!	GTATTAAAGTATGTAAAACA+AGG	-	chr3.1:68096390-68096409	MS.gene72272:intron	20.0%
!!	TACTAGTAAACTTTAACATA+AGG	+	chr3.1:68098441-68098460	None:intergenic	20.0%
!!	TATCATATAGAGAGAATAAA+GGG	-	chr3.1:68098903-68098922	MS.gene72272:intron	20.0%
!!	TGAAGTCAAAAATAAAATGA+TGG	-	chr3.1:68097412-68097431	MS.gene72272:intron	20.0%
!!	TTTAGAAGAAGAAAATTTCA+GGG	-	chr3.1:68098118-68098137	MS.gene72272:CDS	20.0%
!!!	AAGATTGTAAATAGCTATTT+TGG	-	chr3.1:68096415-68096434	MS.gene72272:intron	20.0%
!!!	ACTACAGTAAATAGTTATTT+TGG	+	chr3.1:68096672-68096691	None:intergenic	20.0%
!!!	ACTTTTGAAACTAAAGTAAA+AGG	+	chr3.1:68097462-68097481	None:intergenic	20.0%
!!!	ATTTGTTATTTTTTGGATAG+TGG	-	chr3.1:68098233-68098252	MS.gene72272:CDS	20.0%
!!!	GAAACAAAATTCTGAAATTA+AGG	-	chr3.1:68096003-68096022	MS.gene72272:intron	20.0%
!!!	GAAATTAAGGTTTTTTACTT+AGG	-	chr3.1:68096016-68096035	MS.gene72272:intron	20.0%
!!!	TACTACACTATCAATTTTAA+AGG	-	chr3.1:68096745-68096764	MS.gene72272:intron	20.0%
!!!	TTCAAACATGTCAATTTTAT+AGG	-	chr3.1:68097259-68097278	MS.gene72272:CDS	20.0%
!!!	TTCTAGTATTTTATTTGTGT+TGG	-	chr3.1:68097330-68097349	MS.gene72272:CDS	20.0%
!!!	TTGATCCATTTGTTATTTTT+TGG	-	chr3.1:68098226-68098245	MS.gene72272:CDS	20.0%
!!!	TTTCATTTTCTTGATTTTGA+TGG	-	chr3.1:68095410-68095429	MS.gene72272:CDS	20.0%
!!!	TTTGTTATTTTTTGGATAGT+GGG	-	chr3.1:68098234-68098253	MS.gene72272:CDS	20.0%
!	AGAATAGTCATTGGTAAATA+AGG	+	chr3.1:68098087-68098106	None:intergenic	25.0%
!	AGACAAAAAAGAGTGATTAA+AGG	+	chr3.1:68097211-68097230	None:intergenic	25.0%
!	ATCTAAGGGATATTGTTAAT+AGG	-	chr3.1:68097515-68097534	MS.gene72272:intron	25.0%
!	ATTCTCTCTATATGATACAA+TGG	+	chr3.1:68098900-68098919	None:intergenic	25.0%
!	ATTTCTAATGCTCATACATT+TGG	-	chr3.1:68097084-68097103	MS.gene72272:intron	25.0%
!	CAAAATTGAGAATTTCATCA+TGG	+	chr3.1:68098772-68098791	None:intergenic	25.0%
!	GTATCATATAGAGAGAATAA+AGG	-	chr3.1:68098902-68098921	MS.gene72272:intron	25.0%
!	GTTTAGAAGAAGAAAATTTC+AGG	-	chr3.1:68098117-68098136	MS.gene72272:CDS	25.0%
!	TACTTGTCTCTTAAAGTTAA+AGG	+	chr3.1:68096373-68096392	None:intergenic	25.0%
!	TCAAAATTGTCTAGATTATC+AGG	+	chr3.1:68099016-68099035	None:intergenic	25.0%
!	TGACAATGATGTTTAAATCT+GGG	+	chr3.1:68095193-68095212	None:intergenic	25.0%
!	TGACTAACAAAACATATAAC+AGG	+	chr3.1:68096550-68096569	None:intergenic	25.0%
!	TGCATATATAGAATAGTCAT+TGG	+	chr3.1:68098096-68098115	None:intergenic	25.0%
!	TTTCTTAAAGCCCAAAATAA+AGG	+	chr3.1:68098260-68098279	None:intergenic	25.0%
!!	AGAACCAAATCCTAATATTT+TGG	-	chr3.1:68098970-68098989	MS.gene72272:CDS	25.0%
!!	AGCTATATAGTCTATTCTTT+TGG	+	chr3.1:68097674-68097693	None:intergenic	25.0%
!!	ATGTAATGGAAGCAATTAAA+TGG	-	chr3.1:68095632-68095651	MS.gene72272:CDS	25.0%
!!	ATGTACATTTGAAGTGATAT+TGG	-	chr3.1:68098398-68098417	MS.gene72272:intron	25.0%
!!	GTCAAAATATTCTTGTTTGT+CGG	+	chr3.1:68097058-68097077	None:intergenic	25.0%
!!	TATATAGTCTATTCTTTTGG+AGG	+	chr3.1:68097671-68097690	None:intergenic	25.0%
!!	TGATAATCTAGACAATTTTG+AGG	-	chr3.1:68099015-68099034	MS.gene72272:CDS	25.0%
!!	TGTAATGGAAGCAATTAAAT+GGG	-	chr3.1:68095633-68095652	MS.gene72272:CDS	25.0%
!!	TGTACATTTGAAGTGATATT+GGG	-	chr3.1:68098399-68098418	MS.gene72272:intron	25.0%
!!!	AAATATTAGGATTTGGTTCT+TGG	+	chr3.1:68098970-68098989	None:intergenic	25.0%
!!!	AATTTTAAAGGCAAATAGTG+GGG	-	chr3.1:68096757-68096776	MS.gene72272:intron	25.0%
!!!	ATTAAGGTTTTTTACTTAGG+TGG	-	chr3.1:68096019-68096038	MS.gene72272:intron	25.0%
!!!	ATTTGTTTTCCTAAACACAA+TGG	+	chr3.1:68096180-68096199	None:intergenic	25.0%
!!!	CAATTTTAAAGGCAAATAGT+GGG	-	chr3.1:68096756-68096775	MS.gene72272:intron	25.0%
!!!	TCAATTTTAAAGGCAAATAG+TGG	-	chr3.1:68096755-68096774	MS.gene72272:intron	25.0%
!!!	TCACAAGTTGATTACATTTT+GGG	-	chr3.1:68098797-68098816	MS.gene72272:CDS	25.0%
!!!	TGTTGTTCTTTTAGAAATGT+GGG	+	chr3.1:68098333-68098352	None:intergenic	25.0%
!!!	TTAAGGTTTTTTACTTAGGT+GGG	-	chr3.1:68096020-68096039	MS.gene72272:intron	25.0%
!!!	TTGGAATTTTGACATATTCA+TGG	-	chr3.1:68096614-68096633	MS.gene72272:intron	25.0%
!!!	TTGTTGTTCTTTTAGAAATG+TGG	+	chr3.1:68098334-68098353	None:intergenic	25.0%
	AAAAGATCAATTGCCAAGAT+GGG	-	chr3.1:68098738-68098757	MS.gene72272:CDS	30.0%
	AAAAGTAGCTGAGAATAATG+TGG	+	chr3.1:68096203-68096222	None:intergenic	30.0%
	AAAGCTCCAACCAAAATATT+AGG	+	chr3.1:68098983-68099002	None:intergenic	30.0%
	AAATACAGAGGGAAATATGA+TGG	-	chr3.1:68096943-68096962	MS.gene72272:intron	30.0%
	AATGTAATCAACTTGTGACT+TGG	+	chr3.1:68098795-68098814	None:intergenic	30.0%
	ACCATTGAAACTTCAACATT+TGG	+	chr3.1:68097638-68097657	None:intergenic	30.0%
	ACCCTGAAGAAAACATAAAA+AGG	+	chr3.1:68097992-68098011	None:intergenic	30.0%
	ACTAAGTAACTCATGTTAAG+TGG	+	chr3.1:68098830-68098849	None:intergenic	30.0%
	ACTTAACATGAGTTACTTAG+TGG	-	chr3.1:68098829-68098848	MS.gene72272:intron	30.0%
	ATACATGGATGAGTTATTGT+GGG	-	chr3.1:68097008-68097027	MS.gene72272:intron	30.0%
	ATATAATACTGCACCATTAG+TGG	-	chr3.1:68099075-68099094	MS.gene72272:CDS	30.0%
	ATTATGCATTCACACTTATC+AGG	+	chr3.1:68096709-68096728	None:intergenic	30.0%
	ATTTGATGGAGCAATATCTA+AGG	-	chr3.1:68097500-68097519	MS.gene72272:intron	30.0%
	CCAACCAAAATATTAGGATT+TGG	+	chr3.1:68098977-68098996	None:intergenic	30.0%
	CCCTGAAGAAAACATAAAAA+GGG	+	chr3.1:68097991-68098010	None:intergenic	30.0%
	CTATCACAGTTCAATTAAAG+TGG	-	chr3.1:68097594-68097613	MS.gene72272:intron	30.0%
	CTCTTTGATTAGTAATCTCA+TGG	+	chr3.1:68096580-68096599	None:intergenic	30.0%
	CTTAACATGAGTTACTTAGT+GGG	-	chr3.1:68098830-68098849	MS.gene72272:intron	30.0%
	GAGAAATAAGCCATTGAAAA+AGG	+	chr3.1:68097360-68097379	None:intergenic	30.0%
	GTGAATATAGCACAATTTAG+AGG	+	chr3.1:68096252-68096271	None:intergenic	30.0%
	GTGACAATGATGTTTAAATC+TGG	+	chr3.1:68095194-68095213	None:intergenic	30.0%
	TAATGCACAATGATTGATAG+TGG	-	chr3.1:68098161-68098180	MS.gene72272:CDS	30.0%
	TATAATACTGCACCATTAGT+GGG	-	chr3.1:68099076-68099095	MS.gene72272:CDS	30.0%
	TATCTTAAAAGCTTGTCTTG+AGG	+	chr3.1:68096096-68096115	None:intergenic	30.0%
	TCATGTCTCAACAAAAACAA+TGG	-	chr3.1:68098590-68098609	MS.gene72272:intron	30.0%
	TCTCAAATTATTAGCAGTGT+GGG	+	chr3.1:68095777-68095796	None:intergenic	30.0%
	TTACCTTTGATGCTAAAACT+TGG	+	chr3.1:68097164-68097183	None:intergenic	30.0%
	TTCTCAAATTATTAGCAGTG+TGG	+	chr3.1:68095778-68095797	None:intergenic	30.0%
	TTGCAAAATTCATGCAAAGA+GGG	-	chr3.1:68099104-68099123	MS.gene72272:CDS	30.0%
	TTGTGCTATATTCACTATAG+TGG	-	chr3.1:68096257-68096276	MS.gene72272:intron	30.0%
	TTTGATGGAGCAATATCTAA+GGG	-	chr3.1:68097501-68097520	MS.gene72272:intron	30.0%
	TTTGCAAAATTCATGCAAAG+AGG	-	chr3.1:68099103-68099122	MS.gene72272:CDS	30.0%
!	AAAATATTCTTGTTTGTCGG+TGG	+	chr3.1:68097055-68097074	None:intergenic	30.0%
!	AAGGTGCTTAAAAATGAAGT+GGG	+	chr3.1:68096354-68096373	None:intergenic	30.0%
!	ACCCTTTTTATGTTTTCTTC+AGG	-	chr3.1:68097987-68098006	MS.gene72272:intron	30.0%
!	ATGTTAGTAAAGCATTATGC+AGG	+	chr3.1:68097393-68097412	None:intergenic	30.0%
!	ATTGTGCAGTTATTTGAGTT+TGG	-	chr3.1:68096916-68096935	MS.gene72272:intron	30.0%
!	CCCTTTTTATGTTTTCTTCA+GGG	-	chr3.1:68097988-68098007	MS.gene72272:intron	30.0%
!	GATGGGTTTAAGACTTAATT+TGG	-	chr3.1:68097430-68097449	MS.gene72272:intron	30.0%
!	GTAACTTTTGCAGTTTTTGA+TGG	-	chr3.1:68098499-68098518	MS.gene72272:intron	30.0%
!	GTAATGGAAGCAATTAAATG+GGG	-	chr3.1:68095634-68095653	MS.gene72272:CDS	30.0%
!	TGTGAAATTTGGTTTACCAA+TGG	-	chr3.1:68095534-68095553	MS.gene72272:CDS	30.0%
!	TTACAGATGGTTTAGAACAA+GGG	-	chr3.1:68095365-68095384	MS.gene72272:CDS	30.0%
!	TTGTGCAGTTATTTGAGTTT+GGG	-	chr3.1:68096917-68096936	MS.gene72272:intron	30.0%
!	TTTCCAATTTTTGAACTTCC+TGG	-	chr3.1:68095955-68095974	MS.gene72272:intron	30.0%
!!	AAAGGTGCTTAAAAATGAAG+TGG	+	chr3.1:68096355-68096374	None:intergenic	30.0%
!!	AACTCATGTTAAGTGGATTT+TGG	+	chr3.1:68098823-68098842	None:intergenic	30.0%
!!	TAGACAATTTTGAGGATCAA+AGG	-	chr3.1:68099023-68099042	MS.gene72272:CDS	30.0%
!!	TATTTCTGGAAATGTACTTC+TGG	-	chr3.1:68098355-68098374	MS.gene72272:intron	30.0%
!!	TGATCTTTTGATTCTCACTT+TGG	+	chr3.1:68098727-68098746	None:intergenic	30.0%
!!!	CCAAATCCTAATATTTTGGT+TGG	-	chr3.1:68098974-68098993	MS.gene72272:CDS	30.0%
!!!	GTCACAAGTTGATTACATTT+TGG	-	chr3.1:68098796-68098815	MS.gene72272:CDS	30.0%
!!!	TAATATTTTGGTTGGAGCTT+TGG	-	chr3.1:68098982-68099001	MS.gene72272:CDS	30.0%
!!!	TGTTGGTTTTCCTTTTTCAA+TGG	-	chr3.1:68097347-68097366	MS.gene72272:CDS	30.0%
!!!	TTTTGGGTTTGTGTTTTTTC+AGG	-	chr3.1:68095474-68095493	MS.gene72272:CDS	30.0%
	AAGATAGATGAGAAGAATCC+AGG	-	chr3.1:68096110-68096129	MS.gene72272:intron	35.0%
	ACAGTTCAATTAAAGTGGTC+TGG	-	chr3.1:68097599-68097618	MS.gene72272:intron	35.0%
	AGAGAGAATAAAGGGTTCAT+AGG	-	chr3.1:68098911-68098930	MS.gene72272:intron	35.0%
	AGGGTTTGCAATAAATCAGT+TGG	-	chr3.1:68098007-68098026	MS.gene72272:intron	35.0%
	AGTAAAAGCAAAAAGCAGCA+TGG	+	chr3.1:68097286-68097305	None:intergenic	35.0%
	ATGAGTTACTTAGTGGGATA+TGG	-	chr3.1:68098836-68098855	MS.gene72272:intron	35.0%
	ATTAAAGTGGTCTGGCTATA+TGG	-	chr3.1:68097607-68097626	MS.gene72272:intron	35.0%
	CAAAAGATCAATTGCCAAGA+TGG	-	chr3.1:68098737-68098756	MS.gene72272:CDS	35.0%
	CAACCAGGAAGTTCAAAAAT+TGG	+	chr3.1:68095961-68095980	None:intergenic	35.0%
	CACTTATCAGGAACATTACT+TGG	+	chr3.1:68096697-68096716	None:intergenic	35.0%
	CATACAACCAACGTGTTAAT+TGG	-	chr3.1:68095326-68095345	MS.gene72272:CDS	35.0%
	CATTAATCATCACATCAACC+AGG	+	chr3.1:68095976-68095995	None:intergenic	35.0%
	GAACAATTATGTGCAAACTG+AGG	-	chr3.1:68099045-68099064	MS.gene72272:CDS	35.0%
	GAGAGAATAAAGGGTTCATA+GGG	-	chr3.1:68098912-68098931	MS.gene72272:intron	35.0%
	GATACATGGATGAGTTATTG+TGG	-	chr3.1:68097007-68097026	MS.gene72272:intron	35.0%
	GATTACTCTATGTAAGACAG+TGG	-	chr3.1:68098642-68098661	MS.gene72272:CDS	35.0%
	GCCAAATGTTGAAGTTTCAA+TGG	-	chr3.1:68097634-68097653	MS.gene72272:intron	35.0%
	TACATGGATGAGTTATTGTG+GGG	-	chr3.1:68097009-68097028	MS.gene72272:intron	35.0%
	TCAATCAGATAAGCAATCAG+TGG	+	chr3.1:68097886-68097905	None:intergenic	35.0%
	TCTAATGCTCATACATTTGG+TGG	-	chr3.1:68097087-68097106	MS.gene72272:intron	35.0%
	TGAGTTACTTAGTGGGATAT+GGG	-	chr3.1:68098837-68098856	MS.gene72272:intron	35.0%
	TTACTGTCACAATGTTGTCA+TGG	-	chr3.1:68095560-68095579	MS.gene72272:CDS	35.0%
	TTTGAATTAAGCCAACCTAG+AGG	+	chr3.1:68097245-68097264	None:intergenic	35.0%
	TTTGCAAAAACTCCCACTAA+TGG	+	chr3.1:68099091-68099110	None:intergenic	35.0%
!	AAAACTGCTAGAGCACATTT+TGG	-	chr3.1:68098039-68098058	MS.gene72272:intron	35.0%
!	AAGAGACTCGTGAATGTTTT+TGG	-	chr3.1:68096595-68096614	MS.gene72272:intron	35.0%
!	CAAGGGTATGATAATTGGTA+TGG	-	chr3.1:68098941-68098960	MS.gene72272:CDS	35.0%
!	CAATTAACACGTTGGTTGTA+TGG	+	chr3.1:68095328-68095347	None:intergenic	35.0%
!	CTGGTTGATGTGATGATTAA+TGG	-	chr3.1:68095974-68095993	MS.gene72272:intron	35.0%
!	CTTACAGATGGTTTAGAACA+AGG	-	chr3.1:68095364-68095383	MS.gene72272:CDS	35.0%
!	GACATATTCATGGACTATAG+TGG	-	chr3.1:68096624-68096643	MS.gene72272:intron	35.0%
!	TACAGATGGTTTAGAACAAG+GGG	-	chr3.1:68095366-68095385	MS.gene72272:CDS	35.0%
!	TGTGCAGTTATTTGAGTTTG+GGG	-	chr3.1:68096918-68096937	MS.gene72272:intron	35.0%
!!	ACATGCATTGACTGTCAAAA+TGG	-	chr3.1:68097848-68097867	MS.gene72272:intron	35.0%
!!	ATACATTTGGTGGTATTGGT+TGG	-	chr3.1:68097097-68097116	MS.gene72272:intron	35.0%
!!	ATAGCTTGTGAGACATTTTG+AGG	-	chr3.1:68095880-68095899	MS.gene72272:intron	35.0%
!!	TCTCCAAGTTTTAGCATCAA+AGG	-	chr3.1:68097158-68097177	MS.gene72272:CDS	35.0%
!!!	TAGAGCACATTTTGGTTACT+TGG	-	chr3.1:68098047-68098066	MS.gene72272:intron	35.0%
!!!	TGCTGCTTTTTGCTTTTACT+TGG	-	chr3.1:68097286-68097305	MS.gene72272:CDS	35.0%
!!!	TGTGTTTTTTCAGGTGGATT+TGG	-	chr3.1:68095483-68095502	MS.gene72272:CDS	35.0%
!!!	TTTTTTACTTAGGTGGGTGA+TGG	-	chr3.1:68096026-68096045	MS.gene72272:intron	35.0%
!!!	TTTTTTCAGGTGGATTTGGT+TGG	-	chr3.1:68095487-68095506	MS.gene72272:CDS	35.0%
	ACTATAGTGGCACTAGTGTT+TGG	-	chr3.1:68096270-68096289	MS.gene72272:intron	40.0%
	AGGCAAGAAATTGTTGAAGC+TGG	-	chr3.1:68095598-68095617	MS.gene72272:CDS	40.0%
	AGTTTGGGGACAAATACAGA+GGG	-	chr3.1:68096932-68096951	MS.gene72272:intron	40.0%
	ATACTAGCCCAAATGTGTTG+TGG	-	chr3.1:68095680-68095699	MS.gene72272:CDS	40.0%
	ATCCCAGCTGAATTCTGTTA+TGG	+	chr3.1:68097124-68097143	None:intergenic	40.0%
	ATTGTGACAGTAAACGCCAT+TGG	+	chr3.1:68095553-68095572	None:intergenic	40.0%
	ATTGTTGAAGCTGGTGAACT+TGG	-	chr3.1:68095607-68095626	MS.gene72272:CDS	40.0%
	CAACCTTTCAACTCATCATG+AGG	-	chr3.1:68097923-68097942	MS.gene72272:intron	40.0%
	CATGAGGCAAGCTATTACTA+TGG	-	chr3.1:68097939-68097958	MS.gene72272:intron	40.0%
	CTTACATAGAGTAATCCAGC+TGG	+	chr3.1:68098638-68098657	None:intergenic	40.0%
	GAGACACAGACCATTATTGT+TGG	-	chr3.1:68096048-68096067	MS.gene72272:intron	40.0%
	GATTAGACATGTCACAGTGT+CGG	+	chr3.1:68095841-68095860	None:intergenic	40.0%
	GCAGGTGATCATGTGAAATT+TGG	-	chr3.1:68095523-68095542	MS.gene72272:CDS	40.0%
	GGCATTGTCTCGCATATTTA+AGG	-	chr3.1:68096645-68096664	MS.gene72272:intron	40.0%
	GTCCATAACAGAATTCAGCT+GGG	-	chr3.1:68097119-68097138	MS.gene72272:intron	40.0%
	GTTAATTGGCGTCATCATTC+TGG	-	chr3.1:68095340-68095359	MS.gene72272:CDS	40.0%
	TAACAAATGGATCAAGGGCA+AGG	+	chr3.1:68098221-68098240	None:intergenic	40.0%
	TACTAGCCCAAATGTGTTGT+GGG	-	chr3.1:68095681-68095700	MS.gene72272:CDS	40.0%
	TAGACTGCATCAATTGCACA+TGG	+	chr3.1:68097577-68097596	None:intergenic	40.0%
	TCATGCAAAGAGGGAATAGA+AGG	-	chr3.1:68099113-68099132	MS.gene72272:CDS	40.0%
	TGGTGAACTTGGTCATGTAA+TGG	-	chr3.1:68095618-68095637	MS.gene72272:CDS	40.0%
!	AAGGGTGTATTCCATGAATG+TGG	+	chr3.1:68097973-68097992	None:intergenic	40.0%
!	CATGGAGTGCTATTGAGTTT+AGG	-	chr3.1:68095578-68095597	MS.gene72272:CDS	40.0%
!	GAAAGTTGATCCCTTTGCAT+AGG	+	chr3.1:68098304-68098323	None:intergenic	40.0%
!	GCACACAAGGGTATGATAAT+TGG	-	chr3.1:68098936-68098955	MS.gene72272:CDS	40.0%
!	GCTCATACATTTGGTGGTAT+TGG	-	chr3.1:68097093-68097112	MS.gene72272:intron	40.0%
!	GGGAAATATGATGGAAGTGT+TGG	-	chr3.1:68096952-68096971	MS.gene72272:intron	40.0%
!	GTGCTCTAGCAGTTTTACAA+TGG	+	chr3.1:68098035-68098054	None:intergenic	40.0%
!	TTCAATGGCTTATTTCTCGC+TGG	-	chr3.1:68097362-68097381	MS.gene72272:CDS	40.0%
!	TTGCCTCATGATGAGTTGAA+AGG	+	chr3.1:68097929-68097948	None:intergenic	40.0%
!!	ACTTTGAAGCACAACGTTCT+GGG	-	chr3.1:68095296-68095315	MS.gene72272:CDS	40.0%
!!	CATCATTCTGGTCTTACAGA+TGG	-	chr3.1:68095352-68095371	MS.gene72272:CDS	40.0%
!!	CTTTTAGAAATGTGGGAGTG+TGG	+	chr3.1:68098326-68098345	None:intergenic	40.0%
!!	GCACAATGATTGATAGTGGA+TGG	-	chr3.1:68098165-68098184	MS.gene72272:CDS	40.0%
!!	TACTTTGAAGCACAACGTTC+TGG	-	chr3.1:68095295-68095314	MS.gene72272:CDS	40.0%
!!!	ATTTTGGTTGGAGCTTTGGT+AGG	-	chr3.1:68098986-68099005	MS.gene72272:CDS	40.0%
!!!	GGATAGTGGGCCTTTATTTT+GGG	-	chr3.1:68098247-68098266	MS.gene72272:CDS	40.0%
!!!	TGGATAGTGGGCCTTTATTT+TGG	-	chr3.1:68098246-68098265	MS.gene72272:CDS	40.0%
!!!	TGGGTTTGTGTTTTTTCAGG+TGG	-	chr3.1:68095477-68095496	MS.gene72272:CDS	40.0%
	AAACAATGGCAGCAGCAATG+TGG	-	chr3.1:68098604-68098623	MS.gene72272:intron	45.0%
	AATGTGGAAAGAACACCAGC+TGG	-	chr3.1:68098620-68098639	MS.gene72272:CDS	45.0%
	ACTGTGACATGTCTAATCCG+AGG	-	chr3.1:68095843-68095862	MS.gene72272:intron	45.0%
	AGCTGGGATGTTAAGTATGC+TGG	-	chr3.1:68097135-68097154	MS.gene72272:intron	45.0%
	AGTGAAGAGCTACTATGCGT+CGG	-	chr3.1:68096978-68096997	MS.gene72272:intron	45.0%
	AGTGGCACTAGTGTTTGGTT+TGG	-	chr3.1:68096275-68096294	MS.gene72272:intron	45.0%
	AGTTATTGTGGGGAGCTATG+TGG	-	chr3.1:68097019-68097038	MS.gene72272:intron	45.0%
	CAGCTGCTTCCATTGTGTTT+AGG	-	chr3.1:68096168-68096187	MS.gene72272:intron	45.0%
	CATTTCACGTTCCTCTAGGT+TGG	-	chr3.1:68097231-68097250	MS.gene72272:CDS	45.0%
	CTGGACGTTGCCAACAATAA+TGG	+	chr3.1:68096061-68096080	None:intergenic	45.0%
	GAAGAATCCAGGTTCAGATC+TGG	-	chr3.1:68096121-68096140	MS.gene72272:intron	45.0%
	GAGTTTGGGGACAAATACAG+AGG	-	chr3.1:68096931-68096950	MS.gene72272:intron	45.0%
	GATCCCTTTGCATAGGTAGA+AGG	+	chr3.1:68098297-68098316	None:intergenic	45.0%
	GATGACGCCAATTAACACGT+TGG	+	chr3.1:68095336-68095355	None:intergenic	45.0%
	GCAAAGAGGGAATAGAAGGT+TGG	-	chr3.1:68099117-68099136	MS.gene72272:CDS	45.0%
	GGTCCATAACAGAATTCAGC+TGG	-	chr3.1:68097118-68097137	MS.gene72272:intron	45.0%
	GTCTCACAAGCTATGAAGCA+CGG	+	chr3.1:68095874-68095893	None:intergenic	45.0%
	GTCTCATTTCACGTTCCTCT+AGG	-	chr3.1:68097227-68097246	MS.gene72272:CDS	45.0%
	GTGCCTTCTACCTATGCAAA+GGG	-	chr3.1:68098291-68098310	MS.gene72272:intron	45.0%
	GTGGGGTATGAAGTATGAAC+TGG	-	chr3.1:68096774-68096793	MS.gene72272:intron	45.0%
	TGTGCCTTCTACCTATGCAA+AGG	-	chr3.1:68098290-68098309	MS.gene72272:intron	45.0%
	TTACTACTACCTGCTGAGCA+TGG	+	chr3.1:68096229-68096248	None:intergenic	45.0%
	TTATTGTTGGCAACGTCCAG+AGG	-	chr3.1:68096061-68096080	MS.gene72272:intron	45.0%
!	ACCTCTATGATGCACCCTTT+TGG	+	chr3.1:68098873-68098892	None:intergenic	45.0%
!	CCTCTATGATGCACCCTTTT+GGG	+	chr3.1:68098872-68098891	None:intergenic	45.0%
!	CTCTATGATGCACCCTTTTG+GGG	+	chr3.1:68098871-68098890	None:intergenic	45.0%
!	TTCAGGTGGATTTGGTTGGA+GGG	-	chr3.1:68095491-68095510	MS.gene72272:CDS	45.0%
!	TTTCAGGTGGATTTGGTTGG+AGG	-	chr3.1:68095490-68095509	MS.gene72272:CDS	45.0%
	AATCCAGGTTCAGATCTGGC+TGG	-	chr3.1:68096125-68096144	MS.gene72272:intron	50.0%
	ACCTCTGCCCACAACACATT+TGG	+	chr3.1:68095691-68095710	None:intergenic	50.0%
	ATGGGCCTAAGTACCCCAAA+AGG	-	chr3.1:68098855-68098874	MS.gene72272:intron	50.0%
	CATGGCTCACAATCCCATCT+TGG	+	chr3.1:68098754-68098773	None:intergenic	50.0%
	CCTCTGCCCACAACACATTT+GGG	+	chr3.1:68095690-68095709	None:intergenic	50.0%
	CTTGAGGTTGTCATGTCCTC+TGG	+	chr3.1:68096080-68096099	None:intergenic	50.0%
	GGTTCATAGGGTGCACACAA+GGG	-	chr3.1:68098924-68098943	MS.gene72272:intron	50.0%
	TATGAAGCACGGACACTCCT+CGG	+	chr3.1:68095863-68095882	None:intergenic	50.0%
	TGGAGAAACTGCTGCAGCTA+TGG	-	chr3.1:68096145-68096164	MS.gene72272:intron	50.0%
	TGGGCCTAAGTACCCCAAAA+GGG	-	chr3.1:68098856-68098875	MS.gene72272:intron	50.0%
	TGGTGTGTGCTCCACATTCA+TGG	-	chr3.1:68097959-68097978	MS.gene72272:intron	50.0%
!	ACTTTTGCACCATGCTCAGC+AGG	-	chr3.1:68096217-68096236	MS.gene72272:intron	50.0%
!	CCCAAAAGGGTGCATCATAG+AGG	-	chr3.1:68098869-68098888	MS.gene72272:intron	50.0%
!	TTGAAGCACAACGTTCTGGG+AGG	-	chr3.1:68095299-68095318	MS.gene72272:CDS	50.0%
!	TTGGTTGGAGCTTTGGTAGG+AGG	-	chr3.1:68098989-68099008	MS.gene72272:CDS	50.0%
!!	GGCACTAGTGTTTGGTTTGG+TGG	-	chr3.1:68096278-68096297	MS.gene72272:intron	50.0%
!!	GTTGGAGGGTACTATGATGC+AGG	-	chr3.1:68095505-68095524	MS.gene72272:CDS	50.0%
!!!	TGCACCCTTTTGGGGTACTT+AGG	+	chr3.1:68098863-68098882	None:intergenic	50.0%
	CCCAAATGTGTTGTGGGCAG+AGG	-	chr3.1:68095687-68095706	MS.gene72272:CDS	55.0%
	GGGTTCATAGGGTGCACACA+AGG	-	chr3.1:68098923-68098942	MS.gene72272:intron	55.0%
	TCTCCAGCCAGATCTGAACC+TGG	+	chr3.1:68096131-68096150	None:intergenic	55.0%
!	AGCTACTATGCGTCGGTGAG+TGG	-	chr3.1:68096985-68097004	MS.gene72272:intron	55.0%
!	TGCGTCGGTGAGTGGATACA+TGG	-	chr3.1:68096993-68097012	MS.gene72272:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	68095145	68099177	68095145	ID=MS.gene72272
chr3.1	mRNA	68095145	68099177	68095145	ID=MS.gene72272.t1;Parent=MS.gene72272
chr3.1	exon	68098935	68099177	68098935	ID=MS.gene72272.t1.exon1;Parent=MS.gene72272.t1
chr3.1	CDS	68098935	68099177	68098935	ID=cds.MS.gene72272.t1;Parent=MS.gene72272.t1
chr3.1	exon	68098614	68098826	68098614	ID=MS.gene72272.t1.exon2;Parent=MS.gene72272.t1
chr3.1	CDS	68098614	68098826	68098614	ID=cds.MS.gene72272.t1;Parent=MS.gene72272.t1
chr3.1	exon	68098084	68098284	68098084	ID=MS.gene72272.t1.exon3;Parent=MS.gene72272.t1
chr3.1	CDS	68098084	68098284	68098084	ID=cds.MS.gene72272.t1;Parent=MS.gene72272.t1
chr3.1	exon	68097143	68097397	68097143	ID=MS.gene72272.t1.exon4;Parent=MS.gene72272.t1
chr3.1	CDS	68097143	68097397	68097143	ID=cds.MS.gene72272.t1;Parent=MS.gene72272.t1
chr3.1	exon	68095145	68095810	68095145	ID=MS.gene72272.t1.exon5;Parent=MS.gene72272.t1
chr3.1	CDS	68095145	68095810	68095145	ID=cds.MS.gene72272.t1;Parent=MS.gene72272.t1

Gene Sequence

>MS.gene72272

ATGAAGAATGAGAGACAACAACAACATCATAGAGCTTCTAAAATTCCCAGATTTAAACATCATTGTCACATTTTTCTCTTCATTTTGTTCACTCTTTTTTCCATAACACAATCTTTTGATTATGCTGATGCACTTTCAAAAAGTCTTCTTTACTTTGAAGCACAACGTTCTGGGAGGTTACCATACAACCAACGTGTTAATTGGCGTCATCATTCTGGTCTTACAGATGGTTTAGAACAAGGGGTGAGATCAAAATCATAACTTTTTTCATTTTCTTGATTTTGATGGATTATGATTGTTTTTGTGTAGTTTAAAGTTGAGATTTTTATTTTTGGGTTTGTGTTTTTTCAGGTGGATTTGGTTGGAGGGTACTATGATGCAGGTGATCATGTGAAATTTGGTTTACCAATGGCGTTTACTGTCACAATGTTGTCATGGAGTGCTATTGAGTTTAGGCAAGAAATTGTTGAAGCTGGTGAACTTGGTCATGTAATGGAAGCAATTAAATGGGGAACTGATTATTTCATCAAAGCTCATACTAGCCCAAATGTGTTGTGGGCAGAGGTATATTAAAGTCTATTTTGAATTGATTTATTTGAGTTTACATAAACACATGCGAGACTGACACGCCCACACTGCTAATAATTTGAGAAAATCATATAATTCAGTGTAATTATATGTGTTGTGTCGTGTCCGACACTGTGACATGTCTAATCCGAGGAGTGTCCGTGCTTCATAGCTTGTGAGACATTTTGAGGAAAGTTATGATAAACAGCTAGTAACATGTTCATAAGCTGTTTTTTCAGCTTATTTCCAATTTTTGAACTTCCTGGTTGATGTGATGATTAATGGATTCAAGAAACAAAATTCTGAAATTAAGGTTTTTTACTTAGGTGGGTGATGGAGACACAGACCATTATTGTTGGCAACGTCCAGAGGACATGACAACCTCAAGACAAGCTTTTAAGATAGATGAGAAGAATCCAGGTTCAGATCTGGCTGGAGAAACTGCTGCAGCTATGGCAGCTGCTTCCATTGTGTTTAGGAAAACAAATCCACATTATTCTCAGCTACTTTTGCACCATGCTCAGCAGGTAGTAGTAACCTCTAAATTGTGCTATATTCACTATAGTGGCACTAGTGTTTGGTTTGGTGGTTTAGTTTAAAATATGAATAATTTATTATGTTTGGAGCTAGTTTGCTTTACCCACTTCATTTTTAAGCACCTTTAACTTTAAGAGACAAGTATTAAAGTATGTAAAACAAGGGTAAGATTGTAAATAGCTATTTTGGTCTTTGATTGAGTGAGTTGTTGTTGCATAGTCACATAATGTATCAAAATTACAAAATGTTTTGATTGTGTTTTTTTTTTGGTCAATTTGATTCTTAAACATAGTCACATCCTGTTATATGTTTTGTTAGTCAGTTTGATCCATGAGATTACTAATCAAAGAGACTCGTGAATGTTTTTGGAATTTTGACATATTCATGGACTATAGTGGCATTGTCTCGCATATTTAAGGACCAAAATAACTATTTACTGTAGTTGCCAAGTAATGTTCCTGATAAGTGTGAATGCATAATTTTCTTTTAACAAAACTACTACACTATCAATTTTAAAGGCAAATAGTGGGGTATGAAGTATGAACTGGAAGTAGTTTCAACTGCCAATTAAGTAAAAATATTAGTACAAGTTTAAATTAGTTGTGTTTATATTTTTATAGTAATTGAAAGCAATATTTATTTAATTAGTTAGTTGTATATGTGTACTATTGTGCAGTTATTTGAGTTTGGGGACAAATACAGAGGGAAATATGATGGAAGTGTTGGAGTAGTGAAGAGCTACTATGCGTCGGTGAGTGGATACATGGATGAGTTATTGTGGGGAGCTATGTGGTTGTACAAAGCCACCGACAAACAAGAATATTTTGACTACATTATTTCTAATGCTCATACATTTGGTGGTATTGGTTGGTCCATAACAGAATTCAGCTGGGATGTTAAGTATGCTGGTCTCCAAGTTTTAGCATCAAAGGTAAAATACAAACAAAACAAAAGGGTTTCCTTTAATCACTCTTTTTTGTCTCATTTCACGTTCCTCTAGGTTGGCTTAATTCAAACATGTCAATTTTATAGGTCCATGCTGCTTTTTGCTTTTACTTGGTTTTTTCTTTTCTTTCAAACATTCTAGTATTTTATTTGTGTTGGTTTTCCTTTTTCAATGGCTTATTTCTCGCTGGCTTTGCCTGCATAATGCTTTACTAACATGAAGTCAAAAATAAAATGATGGGTTTAAGACTTAATTTGGTTTGTTCCTTTTACTTTAGTTTCAAAAGTTATTTATCATTTTATTTGATTTGATGGAGCAATATCTAAGGGATATTGTTAATAGGAGTTGAAAATCTTGAAAAGTATACTCAAGAACAATGCCATGTGCAATTGATGCAGTCTATCACAGTTCAATTAAAGTGGTCTGGCTATATGGTTGTGCCAAATGTTGAAGTTTCAATGGTTGCAACAATGCCTCCAAAAGAATAGACTATATAGCTAATATAAATGGTTTGTGAATAGTGCTTATAAACATTATAATTAATTAAAAATTAAGAAACAACAAAATCTTAATTGAAATTATTATTTAAATTTTTTATTTGATTGTATCTATTTTATAAATACTCAGCATGCATGTATAGATTTCACTGATATACATGCATTGACTGTCAAAATGGTAATAATGTATGCCACTGATTGCTTATCTGATTGATAGTATGTCTAAACTCTCAACCTTTCAACTCATCATGAGGCAAGCTATTACTATGGTGTGTGCTCCACATTCATGGAATACACCCTTTTTATGTTTTCTTCAGGGTTTGCAATAAATCAGTTGGATCCATTGTAAAACTGCTAGAGCACATTTTGGTTACTTGGAGTAGCACTATTTTCCTTATTTACCAATGACTATTCTATATATGCATGTTTAGAAGAAGAAAATTTCAGGGTTCATTCATTATGTTGTTAGTAATGCACAATGATTGATAGTGGATGGAAACATGTGATGTAACACATTGTTCACATGCCTTGCCCTTGATCCATTTGTTATTTTTTGGATAGTGGGCCTTTATTTTGGGCTTTAAGAAACACTTTGAAATGTGCCTTCTACCTATGCAAAGGGATCAACTTTCCACACTCCCACATTTCTAAAAGAACAACAAATATTTCTGGAAATGTACTTCTGGACGACACATTTTGTCTGAAAATGTACATTTGAAGTGATATTGGGTGTGCAGAAAGTTCTTCCTTATGTTAAAGTTTACTAGTAAATATGTCTTATAAAGTGAGTGTGATCTAAATGCAATTGTAACTTTTGCAGTTTTTGATGGATGCAAAACACAACAAGCATGCTGATTTACTAGAACAGTACAAATCAAAAGCAGAGTACTATATATGTTCATGTCTCAACAAAAACAATGGCAGCAGCAATGTGGAAAGAACACCAGCTGGATTACTCTATGTAAGACAGTGGAACAACATGCAATATGTTTCTACAGCAGCATTTCTACTCACAGTTTACTCTGATTTTCTCCAAAGTGAGAATCAAAAGATCAATTGCCAAGATGGGATTGTGAGCCATGATGAAATTCTCAATTTTGCCAAGTCACAAGTTGATTACATTTTGGGCCAAAATCCACTTAACATGAGTTACTTAGTGGGATATGGGCCTAAGTACCCCAAAAGGGTGCATCATAGAGGTGCATCCATTGTATCATATAGAGAGAATAAAGGGTTCATAGGGTGCACACAAGGGTATGATAATTGGTATGGTGTCCAAGAACCAAATCCTAATATTTTGGTTGGAGCTTTGGTAGGAGGACCTGATAATCTAGACAATTTTGAGGATCAAAGGAACAATTATGTGCAAACTGAGGCTTGCACATATAATACTGCACCATTAGTGGGAGTTTTTGCAAAATTCATGCAAAGAGGGAATAGAAGGTTGGTTCATGATTCTGATTCACTTTTAGTTGCTTCCTTTTAA

Protein sequence

>MS.gene72272.t1

MKNERQQQHHRASKIPRFKHHCHIFLFILFTLFSITQSFDYADALSKSLLYFEAQRSGRLPYNQRVNWRHHSGLTDGLEQGVDLVGGYYDAGDHVKFGLPMAFTVTMLSWSAIEFRQEIVEAGELGHVMEAIKWGTDYFIKAHTSPNVLWAEVGDGDTDHYCWQRPEDMTTSRQAFKIDEKNPGSDLAGETAAAMAAASIVFRKTNPHYSQLLLHHAQQLFEFGDKYRGKYDGSVGVVKSYYASVSGYMDELLWGAMWLYKATDKQEYFDYIISNAHTFGGIGWSITEFSWDVKYAGLQVLASKFLMDAKHNKHADLLEQYKSKAEYYICSCLNKNNGSSNVERTPAGLLYVRQWNNMQYVSTAAFLLTVYSDFLQSENQKINCQDGIVSHDEILNFAKSQVDYILGQNPLNMSYLVGYGPKYPKRVHHRGASIVSYRENKGFIGCTQGYDNWYGVQEPNPNILVGALVGGPDNLDNFEDQRNNYVQTEACTYNTAPLVGVFAKFMQRGNRRLVHDSDSLLVASF