AlfalfaGEDB | MS.gene59003

M. sativa cultivar XinJiangDaYe / MS.gene59003

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59003.t1	XP_013453854.1	98.8	83	1	0	1	83	276	358	2.80E-37	164.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59003.t1	D4N502	57.5	80	34	0	1	80	281	360	2.0e-20	99.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59003.t1	A0A072UPY1	98.8	83	1	0	1	83	276	358	2.0e-37	164.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04932	MS.gene59003	0.825096	5.65E-54	-1.69E-46
MS.gene049549	MS.gene59003	0.839045	2.00E-57	-1.69E-46
MS.gene050170	MS.gene59003	-0.812078	5.08E-51	-1.69E-46
MS.gene050381	MS.gene59003	0.804522	2.07E-49	-1.69E-46
MS.gene050647	MS.gene59003	0.834137	3.57E-56	-1.69E-46
MS.gene050868	MS.gene59003	-0.808915	2.45E-50	-1.69E-46
MS.gene050871	MS.gene59003	-0.810662	1.03E-50	-1.69E-46
MS.gene051127	MS.gene59003	0.801541	8.58E-49	-1.69E-46
MS.gene051138	MS.gene59003	0.831138	1.98E-55	-1.69E-46
MS.gene051651	MS.gene59003	0.809363	1.96E-50	-1.69E-46
MS.gene051874	MS.gene59003	-0.810539	1.10E-50	-1.69E-46
MS.gene052164	MS.gene59003	-0.804679	1.92E-49	-1.69E-46
MS.gene052211	MS.gene59003	0.827568	1.46E-54	-1.69E-46
MS.gene05240	MS.gene59003	0.847491	1.12E-59	-1.69E-46
MS.gene05244	MS.gene59003	0.813418	2.59E-51	-1.69E-46
MS.gene052606	MS.gene59003	0.836417	9.47E-57	-1.69E-46
MS.gene052805	MS.gene59003	0.80582	1.11E-49	-1.69E-46
MS.gene053031	MS.gene59003	0.813014	3.17E-51	-1.69E-46
MS.gene053033	MS.gene59003	0.800589	1.34E-48	-1.69E-46
MS.gene053403	MS.gene59003	0.805058	1.60E-49	-1.69E-46
MS.gene053406	MS.gene59003	0.830071	3.61E-55	-1.69E-46
MS.gene053420	MS.gene59003	0.845708	3.43E-59	-1.69E-46
MS.gene054146	MS.gene59003	0.81037	1.19E-50	-1.69E-46
MS.gene054181	MS.gene59003	0.853985	1.66E-61	-1.69E-46
MS.gene054706	MS.gene59003	0.826606	2.47E-54	-1.69E-46
MS.gene054707	MS.gene59003	0.805651	1.20E-49	-1.69E-46
MS.gene05470	MS.gene59003	-0.808702	2.72E-50	-1.69E-46
MS.gene054742	MS.gene59003	0.810261	1.26E-50	-1.69E-46
MS.gene055154	MS.gene59003	0.805776	1.13E-49	-1.69E-46
MS.gene055158	MS.gene59003	0.821479	3.95E-53	-1.69E-46
MS.gene055202	MS.gene59003	0.817646	2.96E-52	-1.69E-46
MS.gene055240	MS.gene59003	0.825582	4.33E-54	-1.69E-46
MS.gene05589	MS.gene59003	-0.833125	6.38E-56	-1.69E-46
MS.gene055959	MS.gene59003	0.820676	6.05E-53	-1.69E-46
MS.gene056218	MS.gene59003	0.844226	8.62E-59	-1.69E-46
MS.gene056298	MS.gene59003	0.816516	5.31E-52	-1.69E-46
MS.gene056387	MS.gene59003	-0.818761	1.66E-52	-1.69E-46
MS.gene056504	MS.gene59003	-0.817618	3.00E-52	-1.69E-46
MS.gene056590	MS.gene59003	0.831727	1.42E-55	-1.69E-46
MS.gene056591	MS.gene59003	0.832842	7.50E-56	-1.69E-46
MS.gene056694	MS.gene59003	0.805025	1.63E-49	-1.69E-46
MS.gene056697	MS.gene59003	0.806084	9.77E-50	-1.69E-46
MS.gene056748	MS.gene59003	0.817889	2.61E-52	-1.69E-46
MS.gene056856	MS.gene59003	0.819551	1.09E-52	-1.69E-46
MS.gene057261	MS.gene59003	0.804105	2.53E-49	-1.69E-46
MS.gene057544	MS.gene59003	0.827003	1.99E-54	-1.69E-46
MS.gene057768	MS.gene59003	0.8204	7.00E-53	-1.69E-46
MS.gene057820	MS.gene59003	0.816667	4.91E-52	-1.69E-46
MS.gene058067	MS.gene59003	0.804972	1.67E-49	-1.69E-46
MS.gene058099	MS.gene59003	0.814583	1.43E-51	-1.69E-46
MS.gene058117	MS.gene59003	0.804329	2.28E-49	-1.69E-46
MS.gene058171	MS.gene59003	0.819801	9.60E-53	-1.69E-46
MS.gene058173	MS.gene59003	0.805862	1.09E-49	-1.69E-46
MS.gene058175	MS.gene59003	0.819663	1.03E-52	-1.69E-46
MS.gene05837	MS.gene59003	-0.812285	4.58E-51	-1.69E-46
MS.gene058838	MS.gene59003	-0.814863	1.24E-51	-1.69E-46
MS.gene059037	MS.gene59003	0.803292	3.74E-49	-1.69E-46
MS.gene059402	MS.gene59003	0.8337	4.59E-56	-1.69E-46
MS.gene059667	MS.gene59003	-0.80724	5.57E-50	-1.69E-46
MS.gene059865	MS.gene59003	-0.8003	1.54E-48	-1.69E-46
MS.gene059953	MS.gene59003	0.823773	1.16E-53	-1.69E-46
MS.gene059955	MS.gene59003	0.820977	5.16E-53	-1.69E-46
MS.gene06000	MS.gene59003	-0.809396	1.93E-50	-1.69E-46
MS.gene060087	MS.gene59003	0.803647	3.15E-49	-1.69E-46
MS.gene060372	MS.gene59003	-0.809904	1.50E-50	-1.69E-46
MS.gene060593	MS.gene59003	0.812714	3.69E-51	-1.69E-46
MS.gene060762	MS.gene59003	-0.812079	5.08E-51	-1.69E-46
MS.gene060778	MS.gene59003	-0.80308	4.14E-49	-1.69E-46
MS.gene06077	MS.gene59003	-0.805974	1.03E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59003.t1	MTR_8g064200	96.386	83	3	0	1	83	280	362	4.90e-53	168
MS.gene59003.t1	MTR_8g064200	96.386	83	3	0	1	83	275	357	5.11e-53	168
MS.gene59003.t1	MTR_5g048470	98.795	83	1	0	1	83	276	358	9.36e-53	167
MS.gene59003.t1	MTR_0056s0160	78.313	83	18	0	1	83	154	236	1.06e-43	141
MS.gene59003.t1	MTR_0009s0400	56.627	83	36	0	1	83	65	147	3.38e-34	114
MS.gene59003.t1	MTR_0009s0050	59.036	83	34	0	1	83	89	171	2.39e-33	113
MS.gene59003.t1	MTR_5g055680	57.831	83	35	0	1	83	269	351	8.95e-33	115
MS.gene59003.t1	MTR_5g048550	63.855	83	30	0	1	83	269	351	1.10e-32	115
MS.gene59003.t1	MTR_0009s0050	59.036	83	34	0	1	83	171	253	1.33e-32	113
MS.gene59003.t1	MTR_0009s0050	59.036	83	34	0	1	83	171	253	1.57e-32	113
MS.gene59003.t1	MTR_0009s0050	59.036	83	34	0	1	83	269	351	1.92e-31	112
MS.gene59003.t1	MTR_0009s0050	59.036	83	34	0	1	83	269	351	2.07e-31	112
MS.gene59003.t1	MTR_2g072910	62.651	83	31	0	1	83	275	357	3.91e-31	111
MS.gene59003.t1	MTR_5g048740	57.831	83	35	0	1	83	268	350	1.13e-30	110
MS.gene59003.t1	MTR_0009s0480	54.217	83	38	0	1	83	268	350	4.65e-30	108
MS.gene59003.t1	MTR_5g048570	55.422	83	37	0	1	83	267	349	6.83e-30	108
MS.gene59003.t1	MTR_5g055900	56.627	83	36	0	1	83	269	351	1.16e-29	107
MS.gene59003.t1	MTR_5g055690	59.036	83	34	0	1	83	270	352	1.65e-29	107
MS.gene59003.t1	MTR_5g055820	57.831	83	35	0	1	83	270	352	2.33e-29	107
MS.gene59003.t1	MTR_2g072890	55.422	83	37	0	1	83	276	358	5.34e-29	106
MS.gene59003.t1	MTR_5g048850	59.036	83	34	0	1	83	269	351	1.88e-28	104
MS.gene59003.t1	MTR_5g055800	53.659	82	37	1	1	82	269	349	1.17e-26	99.8
MS.gene59003.t1	MTR_0328s0030	50.602	83	41	0	1	83	267	349	1.94e-26	99.4
MS.gene59003.t1	MTR_1g033810	51.250	80	39	0	1	80	271	350	8.92e-23	89.4
MS.gene59003.t1	MTR_7g105070	32.558	86	54	2	1	82	279	364	4.51e-13	62.8
MS.gene59003.t1	MTR_3g026150	36.250	80	50	1	1	80	179	257	1.22e-12	61.2
MS.gene59003.t1	MTR_3g026150	36.250	80	50	1	1	80	282	360	1.60e-12	61.2
MS.gene59003.t1	MTR_3g063180	34.146	82	54	0	1	82	273	354	1.63e-12	61.2
MS.gene59003.t1	MTR_7g063730	33.333	81	54	0	1	81	286	366	2.62e-12	60.8
MS.gene59003.t1	MTR_2g437380	33.333	81	54	0	1	81	277	357	3.81e-12	60.1
MS.gene59003.t1	MTR_2g437380	33.333	81	54	0	1	81	183	263	6.33e-12	59.3
MS.gene59003.t1	MTR_5g065010	35.802	81	51	1	1	80	262	342	7.15e-12	59.3
MS.gene59003.t1	MTR_3g070860	31.646	79	54	0	1	79	278	356	8.01e-12	59.3
MS.gene59003.t1	MTR_4g015790	36.667	60	38	0	1	60	291	350	2.51e-11	57.8
MS.gene59003.t1	MTR_4g123020	40.580	69	41	0	1	69	242	310	3.92e-11	57.4
MS.gene59003.t1	MTR_8g024120	32.895	76	51	0	1	76	264	339	4.80e-11	57.0
MS.gene59003.t1	MTR_7g062550	31.325	83	57	0	1	83	282	364	5.77e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59003.t1	AT4G25300	50.000	80	40	0	1	80	183	262	1.41e-24	92.8
MS.gene59003.t1	AT4G25300	50.000	80	40	0	1	80	277	356	1.58e-23	91.7
MS.gene59003.t1	AT4G25310	48.750	80	41	0	1	80	246	325	5.84e-23	89.7
MS.gene59003.t1	AT1G17010	48.193	83	43	0	1	83	279	361	8.94e-23	89.7
MS.gene59003.t1	AT4G25310	48.750	80	41	0	1	80	274	353	9.39e-23	89.4
MS.gene59003.t1	AT1G78550	46.250	80	43	0	1	80	199	278	2.63e-21	84.7
MS.gene59003.t1	AT1G78550	46.250	80	43	0	1	80	277	356	1.11e-20	84.0
MS.gene59003.t1	AT1G17020	47.500	80	42	0	1	80	279	358	3.42e-20	82.4
MS.gene59003.t1	AT3G60290	37.349	83	50	2	1	82	275	356	2.54e-14	66.2
MS.gene59003.t1	AT2G44800	34.177	79	52	0	1	79	275	353	4.31e-13	62.8
MS.gene59003.t1	AT3G13610	31.579	76	52	0	1	76	282	357	1.12e-11	58.9
MS.gene59003.t1	AT3G21420	33.735	83	54	1	1	82	282	364	3.21e-11	57.8
MS.gene59003.t1	AT2G36690	32.500	80	54	0	1	80	284	363	3.37e-11	57.8
MS.gene59003.t1	AT2G36690	32.500	80	54	0	1	80	284	363	3.37e-11	57.8
MS.gene59003.t1	AT4G16330	34.615	78	47	1	1	78	183	256	3.40e-11	57.4
MS.gene59003.t1	AT3G55970	29.114	79	56	0	1	79	282	360	3.53e-11	57.4
MS.gene59003.t1	AT5G07480	31.325	83	56	1	1	82	274	356	4.11e-11	57.4
MS.gene59003.t1	AT2G38240	36.667	60	38	0	1	60	272	331	4.18e-11	57.4
MS.gene59003.t1	AT4G16330	34.615	78	47	1	1	78	289	362	7.38e-11	56.6
MS.gene59003.t1	AT5G07480	31.325	83	56	1	1	82	165	247	8.66e-11	55.8

Find 13 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGTAATAAGGCTTGGTAC+TGG	0.450966	5.3:-37007264	None:intergenic
TATTTCTCACGCCAGCTTGA+AGG	0.471903	5.3:+37007339	MS.gene59003:CDS
CTAATGTCGTTGAACACTGC+AGG	0.481272	5.3:-37007294	None:intergenic
AGTGTGGAAGATTTCTTCAA+AGG	0.508864	5.3:+37007315	MS.gene59003:CDS
TAATGTCGTTGAACACTGCA+GGG	0.582551	5.3:-37007293	None:intergenic
CAATCTAGCACTGTGAAATG+TGG	0.587201	5.3:-37007226	None:intergenic
CGATTAACTCAGAGAAGGAG+AGG	0.590103	5.3:+37007193	MS.gene59003:CDS
AGCACTGTGAAATGTGGCAA+TGG	0.625118	5.3:-37007220	None:intergenic
GTTTGACAGATAATGACGAA+TGG	0.632619	5.3:+37007141	None:intergenic
AGCAACGATTAACTCAGAGA+AGG	0.647001	5.3:+37007188	MS.gene59003:CDS
AAATGTACATTGATGACATG+AGG	0.655321	5.3:+37007364	MS.gene59003:CDS
AATGTCGTTGAACACTGCAG+GGG	0.691919	5.3:-37007292	None:intergenic
AGTGTTCAACGACATTAGTG+TGG	0.716232	5.3:+37007299	MS.gene59003:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAATGTACATTGATGACATG+AGG	+	chr5.3:37007364-37007383	MS.gene59003:CDS	30.0%
	AGTGTGGAAGATTTCTTCAA+AGG	+	chr5.3:37007315-37007334	MS.gene59003:CDS	35.0%
!	TGTACATTTTTCCTTCAAGC+TGG	-	chr5.3:37007353-37007372	None:intergenic	35.0%
!!!	GTTTTAGGAGTAATAAGGCT+TGG	-	chr5.3:37007273-37007292	None:intergenic	35.0%
	AGCAACGATTAACTCAGAGA+AGG	+	chr5.3:37007188-37007207	MS.gene59003:CDS	40.0%
	AGTGTTCAACGACATTAGTG+TGG	+	chr5.3:37007299-37007318	MS.gene59003:CDS	40.0%
	CAATCTAGCACTGTGAAATG+TGG	-	chr5.3:37007229-37007248	None:intergenic	40.0%
	TAATGTCGTTGAACACTGCA+GGG	-	chr5.3:37007296-37007315	None:intergenic	40.0%
!!!	CAGGGGTTTTAGGAGTAATA+AGG	-	chr5.3:37007278-37007297	None:intergenic	40.0%
	AATGTCGTTGAACACTGCAG+GGG	-	chr5.3:37007295-37007314	None:intergenic	45.0%
	AGCACTGTGAAATGTGGCAA+TGG	-	chr5.3:37007223-37007242	None:intergenic	45.0%
	CGATTAACTCAGAGAAGGAG+AGG	+	chr5.3:37007193-37007212	MS.gene59003:CDS	45.0%
	CTAATGTCGTTGAACACTGC+AGG	-	chr5.3:37007297-37007316	None:intergenic	45.0%
	TATTTCTCACGCCAGCTTGA+AGG	+	chr5.3:37007339-37007358	MS.gene59003:CDS	45.0%
!	GGAGTAATAAGGCTTGGTAC+TGG	-	chr5.3:37007267-37007286	None:intergenic	45.0%
!	TTGAACACTGCAGGGGTTTT+AGG	-	chr5.3:37007288-37007307	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	37007150	37007401	37007150	ID=MS.gene59003
chr5.3	mRNA	37007150	37007401	37007150	ID=MS.gene59003.t1;Parent=MS.gene59003
chr5.3	exon	37007150	37007401	37007150	ID=MS.gene59003.t1.exon1;Parent=MS.gene59003.t1
chr5.3	CDS	37007150	37007401	37007150	ID=cds.MS.gene59003.t1;Parent=MS.gene59003.t1

Gene Sequence	>MS.gene59003 ATAATGACGAATGGTATTTATCGAAGTATTGAACATCGAGCAACGATTAACTCAGAGAAGGAGAGGATTTCCATTGCCACATTTCACAGTGCTAGATTGAATGCAATTCTTGCTCCAGTACCAAGCCTTATTACTCCTAAAACCCCTGCAGTGTTCAACGACATTAGTGTGGAAGATTTCTTCAAAGGATATTTCTCACGCCAGCTTGAAGGAAAAATGTACATTGATGACATGAGGATGAAAAATGAGTGA
Protein sequence	>MS.gene59003.t1 IMTNGIYRSIEHRATINSEKERISIATFHSARLNAILAPVPSLITPKTPAVFNDISVEDFFKGYFSRQLEGKMYIDDMRMKNE