Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69546.t1 | XP_003599832.1 | 91.3 | 173 | 15 | 0 | 3 | 175 | 8 | 180 | 8.20E-79 | 303.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69546.t1 | G7IXV5 | 91.3 | 173 | 15 | 0 | 3 | 175 | 8 | 180 | 5.9e-79 | 303.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene69546 | 0.808251 | 3.39E-50 | -1.69E-46 |
| MS.gene049404 | MS.gene69546 | 0.80905 | 2.29E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene69546 | 0.804333 | 2.27E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene69546 | 0.804184 | 2.44E-49 | -1.69E-46 |
| MS.gene050237 | MS.gene69546 | 0.825012 | 5.91E-54 | -1.69E-46 |
| MS.gene050303 | MS.gene69546 | 0.807376 | 5.21E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene69546 | 0.801859 | 7.38E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene69546 | 0.800299 | 1.54E-48 | -1.69E-46 |
| MS.gene050719 | MS.gene69546 | 0.804285 | 2.32E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene69546 | 0.805024 | 1.63E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene69546 | 0.81432 | 1.64E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene69546 | 0.800548 | 1.37E-48 | -1.69E-46 |
| MS.gene051744 | MS.gene69546 | 0.803085 | 4.12E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene69546 | 0.810016 | 1.42E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene69546 | 0.835167 | 1.96E-56 | -1.69E-46 |
| MS.gene052582 | MS.gene69546 | 0.826207 | 3.08E-54 | -1.69E-46 |
| MS.gene052584 | MS.gene69546 | 0.832775 | 7.80E-56 | -1.69E-46 |
| MS.gene052773 | MS.gene69546 | 0.815033 | 1.14E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene69546 | 0.833391 | 5.48E-56 | -1.69E-46 |
| MS.gene053008 | MS.gene69546 | 0.812184 | 4.82E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene69546 | 0.83395 | 3.97E-56 | -1.69E-46 |
| MS.gene053883 | MS.gene69546 | 0.802486 | 5.48E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene69546 | 0.815185 | 1.05E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene69546 | 0.821704 | 3.50E-53 | -1.69E-46 |
| MS.gene054657 | MS.gene69546 | 0.814463 | 1.52E-51 | -1.69E-46 |
| MS.gene05517 | MS.gene69546 | 0.818781 | 1.64E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene69546 | 0.814416 | 1.56E-51 | -1.69E-46 |
| MS.gene055468 | MS.gene69546 | 0.811787 | 5.88E-51 | -1.69E-46 |
| MS.gene055808 | MS.gene69546 | 0.803339 | 3.66E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene69546 | 0.835649 | 1.48E-56 | -1.69E-46 |
| MS.gene055841 | MS.gene69546 | 0.811645 | 6.31E-51 | -1.69E-46 |
| MS.gene056162 | MS.gene69546 | 0.82513 | 5.54E-54 | -1.69E-46 |
| MS.gene056174 | MS.gene69546 | 0.825395 | 4.80E-54 | -1.69E-46 |
| MS.gene056215 | MS.gene69546 | 0.829316 | 5.51E-55 | -1.69E-46 |
| MS.gene056366 | MS.gene69546 | 0.827377 | 1.62E-54 | -1.69E-46 |
| MS.gene056595 | MS.gene69546 | 0.80988 | 1.52E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene69546 | 0.827456 | 1.55E-54 | -1.69E-46 |
| MS.gene057060 | MS.gene69546 | 0.82905 | 6.39E-55 | -1.69E-46 |
| MS.gene057208 | MS.gene69546 | 0.80854 | 2.95E-50 | -1.69E-46 |
| MS.gene057314 | MS.gene69546 | 0.812146 | 4.91E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene69546 | 0.820989 | 5.12E-53 | -1.69E-46 |
| MS.gene057693 | MS.gene69546 | 0.801217 | 9.99E-49 | -1.69E-46 |
| MS.gene058009 | MS.gene69546 | 0.806014 | 1.01E-49 | -1.69E-46 |
| MS.gene058548 | MS.gene69546 | 0.800683 | 1.28E-48 | -1.69E-46 |
| MS.gene058636 | MS.gene69546 | 0.805923 | 1.06E-49 | -1.69E-46 |
| MS.gene058783 | MS.gene69546 | 0.809879 | 1.52E-50 | -1.69E-46 |
| MS.gene058829 | MS.gene69546 | 0.807416 | 5.11E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene69546 | 0.806825 | 6.81E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene69546 | 0.832323 | 1.01E-55 | -1.69E-46 |
| MS.gene059625 | MS.gene69546 | 0.813006 | 3.18E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene69546 | 0.819424 | 1.17E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene69546 | 0.808522 | 2.97E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene69546 | 0.806324 | 8.69E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene69546 | 0.836409 | 9.52E-57 | -1.69E-46 |
| MS.gene060521 | MS.gene69546 | 0.821326 | 4.29E-53 | -1.69E-46 |
| MS.gene060572 | MS.gene69546 | 0.828571 | 8.35E-55 | -1.69E-46 |
| MS.gene060587 | MS.gene69546 | 0.813682 | 2.26E-51 | -1.69E-46 |
| MS.gene060634 | MS.gene69546 | -0.813535 | 2.44E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene69546 | 0.81487 | 1.24E-51 | -1.69E-46 |
| MS.gene060917 | MS.gene69546 | 0.821486 | 3.94E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69546.t1 | MTR_3g047590 | 91.329 | 173 | 15 | 0 | 3 | 175 | 8 | 180 | 6.70e-114 | 321 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69546.t1 | AT1G06500 | 40.789 | 152 | 68 | 4 | 22 | 173 | 2 | 131 | 3.42e-30 | 107 |
| MS.gene69546.t1 | AT1G06500 | 40.789 | 152 | 68 | 4 | 22 | 173 | 2 | 131 | 3.42e-30 | 107 |
| MS.gene69546.t1 | AT1G06500 | 40.789 | 152 | 68 | 4 | 22 | 173 | 2 | 131 | 3.42e-30 | 107 |
| MS.gene69546.t1 | AT1G06500 | 40.789 | 152 | 68 | 4 | 22 | 173 | 2 | 131 | 3.42e-30 | 107 |
Find 45 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATTGCTCCATCAAATATT+TGG | 0.194483 | 3.1:+33719697 | MS.gene69546:CDS |
| ACATTGGATGAATTTAAATA+TGG | 0.196305 | 3.1:+33719382 | MS.gene69546:CDS |
| CATGCATCCATCCATGATTT+TGG | 0.208551 | 3.1:-33719730 | None:intergenic |
| TTCCTTGTTGATGTGAATTC+TGG | 0.218734 | 3.1:-33719294 | None:intergenic |
| GCCTCTCCTCCGCTTTCTTC+TGG | 0.239178 | 3.1:-33719505 | None:intergenic |
| GGAAGAGAAACATTTAAATT+AGG | 0.257008 | 3.1:+33719628 | MS.gene69546:CDS |
| TATCGACTACTTCAAATAAA+TGG | 0.302615 | 3.1:+33719422 | MS.gene69546:CDS |
| ACACCGTGTTCGGAAAATTC+TGG | 0.307317 | 3.1:-33719342 | None:intergenic |
| ATCCAATGTTGTCATTCCTT+TGG | 0.364028 | 3.1:-33719368 | None:intergenic |
| CATTGGATGAATTTAAATAT+GGG | 0.366867 | 3.1:+33719383 | MS.gene69546:CDS |
| TGAAGTAGTCGATAAGCTTT+CGG | 0.377284 | 3.1:-33719413 | None:intergenic |
| GATCTTCATTGCCAAAATCA+TGG | 0.401657 | 3.1:+33719719 | MS.gene69546:CDS |
| CCTTTGGCTAACACCGTGTT+CGG | 0.405151 | 3.1:-33719352 | None:intergenic |
| CACCGTGTTCGGAAAATTCT+GGG | 0.447219 | 3.1:-33719341 | None:intergenic |
| ATGAAGATCCAAATATTTGA+TGG | 0.453836 | 3.1:-33719705 | None:intergenic |
| GTTGACTCTTGCCTCCGTCT+TGG | 0.455211 | 3.1:-33719483 | None:intergenic |
| CGGAGGAGAGGCAGAGAAGA+AGG | 0.461047 | 3.1:+33719516 | MS.gene69546:CDS |
| GTGTAAACAAGCTAGGCAAA+AGG | 0.464270 | 3.1:+33719668 | MS.gene69546:CDS |
| AGGAAAAGAGCTGTAGAAGA+AGG | 0.487958 | 3.1:+33719607 | MS.gene69546:CDS |
| TTAGGTGTTCTCCGAGGCTA+TGG | 0.498800 | 3.1:+33719646 | MS.gene69546:CDS |
| GACAAAAGCACAAGGTGTTA+CGG | 0.499816 | 3.1:+33719267 | MS.gene69546:CDS |
| GACTACTTCAAATAAATGGT+GGG | 0.503994 | 3.1:+33719426 | MS.gene69546:CDS |
| GAGAAGAAGGAATGTGAAAC+CGG | 0.508809 | 3.1:+33719529 | MS.gene69546:CDS |
| GGAAATAAAAGCGTTGAAAG+TGG | 0.510593 | 3.1:+33719313 | MS.gene69546:CDS |
| CTTCAAATAAATGGTGGGGA+TGG | 0.520192 | 3.1:+33719431 | MS.gene69546:CDS |
| TGTAAACAAGCTAGGCAAAA+GGG | 0.535406 | 3.1:+33719669 | MS.gene69546:CDS |
| ACCAGAAGAAAGCGGAGGAG+AGG | 0.544142 | 3.1:+33719504 | MS.gene69546:CDS |
| TTCATTGCCAAAATCATGGA+TGG | 0.547716 | 3.1:+33719723 | MS.gene69546:CDS |
| CGACTACTTCAAATAAATGG+TGG | 0.553601 | 3.1:+33719425 | MS.gene69546:CDS |
| CCGAACACGGTGTTAGCCAA+AGG | 0.554812 | 3.1:+33719352 | MS.gene69546:CDS |
| GCTTGTTTACACCATAGCCT+CGG | 0.602789 | 3.1:-33719657 | None:intergenic |
| GGCTATGGTGTAAACAAGCT+AGG | 0.604381 | 3.1:+33719661 | MS.gene69546:CDS |
| ACTACTTCAAATAAATGGTG+GGG | 0.619643 | 3.1:+33719427 | MS.gene69546:CDS |
| TGCAACAAGCAAATGATGGG+AGG | 0.634860 | 3.1:+33719245 | MS.gene69546:CDS |
| AGTCAACCAGAAGAAAGCGG+AGG | 0.636989 | 3.1:+33719499 | MS.gene69546:CDS |
| AAGAGTCAACCAGAAGAAAG+CGG | 0.639731 | 3.1:+33719496 | MS.gene69546:CDS |
| AGCCAAAGGAATGACAACAT+TGG | 0.640708 | 3.1:+33719366 | MS.gene69546:CDS |
| CACCAGAATTCACATCAACA+AGG | 0.644361 | 3.1:+33719292 | MS.gene69546:CDS |
| GTAGTCGATAAGCTTTCGGA+CGG | 0.669763 | 3.1:-33719409 | None:intergenic |
| CATCTTTGTTGAGATCTGTG+AGG | 0.675369 | 3.1:+33719587 | MS.gene69546:CDS |
| AGTAGTGAAGAAACTCGTCA+AGG | 0.678227 | 3.1:+33719562 | MS.gene69546:CDS |
| CGTACTGACACAAACCAAGA+CGG | 0.693330 | 3.1:+33719469 | MS.gene69546:CDS |
| GATGGGAGGACAAAAGCACA+AGG | 0.714490 | 3.1:+33719259 | MS.gene69546:CDS |
| TTTAAATTAGGTGTTCTCCG+AGG | 0.721425 | 3.1:+33719640 | MS.gene69546:CDS |
| ACTGACACAAACCAAGACGG+AGG | 0.740979 | 3.1:+33719472 | MS.gene69546:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATTGGATGAATTTAAATA+TGG | + | chr3.1:33719382-33719401 | MS.gene69546:CDS | 20.0% |
| !! | CATTGGATGAATTTAAATAT+GGG | + | chr3.1:33719383-33719402 | MS.gene69546:CDS | 20.0% |
| ! | ATATTGCTCCATCAAATATT+TGG | + | chr3.1:33719697-33719716 | MS.gene69546:CDS | 25.0% |
| ! | ATGAAGATCCAAATATTTGA+TGG | - | chr3.1:33719708-33719727 | None:intergenic | 25.0% |
| ! | GGAAGAGAAACATTTAAATT+AGG | + | chr3.1:33719628-33719647 | MS.gene69546:CDS | 25.0% |
| !! | TATCGACTACTTCAAATAAA+TGG | + | chr3.1:33719422-33719441 | MS.gene69546:CDS | 25.0% |
| ACTACTTCAAATAAATGGTG+GGG | + | chr3.1:33719427-33719446 | MS.gene69546:CDS | 30.0% | |
| GACTACTTCAAATAAATGGT+GGG | + | chr3.1:33719426-33719445 | MS.gene69546:CDS | 30.0% | |
| ! | TTCACTACTTTCAACTTTTC+CGG | - | chr3.1:33719551-33719570 | None:intergenic | 30.0% |
| ! | TTTTGCAACAAGCAAATGAT+GGG | + | chr3.1:33719242-33719261 | MS.gene69546:CDS | 30.0% |
| ATCCAATGTTGTCATTCCTT+TGG | - | chr3.1:33719371-33719390 | None:intergenic | 35.0% | |
| CGACTACTTCAAATAAATGG+TGG | + | chr3.1:33719425-33719444 | MS.gene69546:CDS | 35.0% | |
| GATCTTCATTGCCAAAATCA+TGG | + | chr3.1:33719719-33719738 | MS.gene69546:CDS | 35.0% | |
| TGAAGTAGTCGATAAGCTTT+CGG | - | chr3.1:33719416-33719435 | None:intergenic | 35.0% | |
| TGTAAACAAGCTAGGCAAAA+GGG | + | chr3.1:33719669-33719688 | MS.gene69546:CDS | 35.0% | |
| TTCATTGCCAAAATCATGGA+TGG | + | chr3.1:33719723-33719742 | MS.gene69546:CDS | 35.0% | |
| TTCCTTGTTGATGTGAATTC+TGG | - | chr3.1:33719297-33719316 | None:intergenic | 35.0% | |
| TTTAAATTAGGTGTTCTCCG+AGG | + | chr3.1:33719640-33719659 | MS.gene69546:CDS | 35.0% | |
| ! | GTTTTGCAACAAGCAAATGA+TGG | + | chr3.1:33719241-33719260 | MS.gene69546:CDS | 35.0% |
| !! | GGAAATAAAAGCGTTGAAAG+TGG | + | chr3.1:33719313-33719332 | MS.gene69546:CDS | 35.0% |
| AAGAGTCAACCAGAAGAAAG+CGG | + | chr3.1:33719496-33719515 | MS.gene69546:CDS | 40.0% | |
| AGCCAAAGGAATGACAACAT+TGG | + | chr3.1:33719366-33719385 | MS.gene69546:CDS | 40.0% | |
| AGGAAAAGAGCTGTAGAAGA+AGG | + | chr3.1:33719607-33719626 | MS.gene69546:CDS | 40.0% | |
| AGTAGTGAAGAAACTCGTCA+AGG | + | chr3.1:33719562-33719581 | MS.gene69546:CDS | 40.0% | |
| CACCAGAATTCACATCAACA+AGG | + | chr3.1:33719292-33719311 | MS.gene69546:CDS | 40.0% | |
| CATCTTTGTTGAGATCTGTG+AGG | + | chr3.1:33719587-33719606 | MS.gene69546:CDS | 40.0% | |
| GAGAAGAAGGAATGTGAAAC+CGG | + | chr3.1:33719529-33719548 | MS.gene69546:CDS | 40.0% | |
| GTGTAAACAAGCTAGGCAAA+AGG | + | chr3.1:33719668-33719687 | MS.gene69546:CDS | 40.0% | |
| ! | CATGCATCCATCCATGATTT+TGG | - | chr3.1:33719733-33719752 | None:intergenic | 40.0% |
| ! | CTTCAAATAAATGGTGGGGA+TGG | + | chr3.1:33719431-33719450 | MS.gene69546:CDS | 40.0% |
| ! | GACAAAAGCACAAGGTGTTA+CGG | + | chr3.1:33719267-33719286 | MS.gene69546:CDS | 40.0% |
| ACACCGTGTTCGGAAAATTC+TGG | - | chr3.1:33719345-33719364 | None:intergenic | 45.0% | |
| CACCGTGTTCGGAAAATTCT+GGG | - | chr3.1:33719344-33719363 | None:intergenic | 45.0% | |
| GCTTGTTTACACCATAGCCT+CGG | - | chr3.1:33719660-33719679 | None:intergenic | 45.0% | |
| GGCTATGGTGTAAACAAGCT+AGG | + | chr3.1:33719661-33719680 | MS.gene69546:CDS | 45.0% | |
| GTAGTCGATAAGCTTTCGGA+CGG | - | chr3.1:33719412-33719431 | None:intergenic | 45.0% | |
| ! | CGTACTGACACAAACCAAGA+CGG | + | chr3.1:33719469-33719488 | MS.gene69546:CDS | 45.0% |
| ! | TGCAACAAGCAAATGATGGG+AGG | + | chr3.1:33719245-33719264 | MS.gene69546:CDS | 45.0% |
| ACTGACACAAACCAAGACGG+AGG | + | chr3.1:33719472-33719491 | MS.gene69546:CDS | 50.0% | |
| AGTCAACCAGAAGAAAGCGG+AGG | + | chr3.1:33719499-33719518 | MS.gene69546:CDS | 50.0% | |
| CCTTTGGCTAACACCGTGTT+CGG | - | chr3.1:33719355-33719374 | None:intergenic | 50.0% | |
| TTAGGTGTTCTCCGAGGCTA+TGG | + | chr3.1:33719646-33719665 | MS.gene69546:CDS | 50.0% | |
| ! | CGCCCAGAATTTTCCGAACA+CGG | + | chr3.1:33719339-33719358 | MS.gene69546:CDS | 50.0% |
| ! | GATGGGAGGACAAAAGCACA+AGG | + | chr3.1:33719259-33719278 | MS.gene69546:CDS | 50.0% |
| ACCAGAAGAAAGCGGAGGAG+AGG | + | chr3.1:33719504-33719523 | MS.gene69546:CDS | 55.0% | |
| ! | CCGAACACGGTGTTAGCCAA+AGG | + | chr3.1:33719352-33719371 | MS.gene69546:CDS | 55.0% |
| ! | GTTGACTCTTGCCTCCGTCT+TGG | - | chr3.1:33719486-33719505 | None:intergenic | 55.0% |
| CGGAGGAGAGGCAGAGAAGA+AGG | + | chr3.1:33719516-33719535 | MS.gene69546:CDS | 60.0% | |
| GCCTCTCCTCCGCTTTCTTC+TGG | - | chr3.1:33719508-33719527 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 33719226 | 33719753 | 33719226 | ID=MS.gene69546 |
| chr3.1 | mRNA | 33719226 | 33719753 | 33719226 | ID=MS.gene69546.t1;Parent=MS.gene69546 |
| chr3.1 | exon | 33719226 | 33719753 | 33719226 | ID=MS.gene69546.t1.exon1;Parent=MS.gene69546.t1 |
| chr3.1 | CDS | 33719226 | 33719753 | 33719226 | ID=cds.MS.gene69546.t1;Parent=MS.gene69546.t1 |
| Gene Sequence |
| Protein sequence |
