AlfalfaGEDB | MS.gene75508

M. sativa cultivar XinJiangDaYe / MS.gene75508

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75508.t1	XP_003601922.1	94.2	154	9	0	1	154	1	154	1.10E-76	295.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75508.t1	G7J380	94.2	154	9	0	1	154	1	154	8.3e-77	295.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049181	MS.gene75508	0.833214	6.06E-56	-1.69E-46
MS.gene049281	MS.gene75508	0.850599	1.53E-60	-1.69E-46
MS.gene05015	MS.gene75508	0.805397	1.36E-49	-1.69E-46
MS.gene050363	MS.gene75508	0.825949	3.55E-54	-1.69E-46
MS.gene050381	MS.gene75508	0.845147	4.87E-59	-1.69E-46
MS.gene050496	MS.gene75508	-0.801079	1.07E-48	-1.69E-46
MS.gene050883	MS.gene75508	0.886839	2.41E-72	-1.69E-46
MS.gene051044	MS.gene75508	0.822688	2.07E-53	-1.69E-46
MS.gene051127	MS.gene75508	0.847395	1.19E-59	-1.69E-46
MS.gene051364	MS.gene75508	0.807213	5.64E-50	-1.69E-46
MS.gene051946	MS.gene75508	0.82554	4.43E-54	-1.69E-46
MS.gene052053	MS.gene75508	0.809412	1.92E-50	-1.69E-46
MS.gene052089	MS.gene75508	0.840222	9.90E-58	-1.69E-46
MS.gene052091	MS.gene75508	0.804849	1.77E-49	-1.69E-46
MS.gene052482	MS.gene75508	-0.807287	5.44E-50	-1.69E-46
MS.gene052486	MS.gene75508	-0.809379	1.95E-50	-1.69E-46
MS.gene053420	MS.gene75508	0.813111	3.02E-51	-1.69E-46
MS.gene053549	MS.gene75508	0.814253	1.69E-51	-1.69E-46
MS.gene053561	MS.gene75508	0.80486	1.76E-49	-1.69E-46
MS.gene053826	MS.gene75508	0.842925	1.92E-58	-1.69E-46
MS.gene054056	MS.gene75508	0.831159	1.95E-55	-1.69E-46
MS.gene054959	MS.gene75508	0.805045	1.61E-49	-1.69E-46
MS.gene055154	MS.gene75508	0.824751	6.81E-54	-1.69E-46
MS.gene055549	MS.gene75508	0.874617	5.87E-68	-1.69E-46
MS.gene055550	MS.gene75508	0.865093	7.58E-65	-1.69E-46
MS.gene055551	MS.gene75508	0.837875	4.01E-57	-1.69E-46
MS.gene05589	MS.gene75508	-0.808025	3.79E-50	-1.69E-46
MS.gene055942	MS.gene75508	0.825562	4.38E-54	-1.69E-46
MS.gene056310	MS.gene75508	0.827602	1.43E-54	-1.69E-46
MS.gene056311	MS.gene75508	0.865637	5.11E-65	-1.69E-46
MS.gene056365	MS.gene75508	0.813661	2.29E-51	-1.69E-46
MS.gene056504	MS.gene75508	-0.8236	1.27E-53	-1.69E-46
MS.gene056761	MS.gene75508	0.814873	1.23E-51	-1.69E-46
MS.gene057014	MS.gene75508	0.831257	1.85E-55	-1.69E-46
MS.gene058068	MS.gene75508	0.822897	1.85E-53	-1.69E-46
MS.gene058099	MS.gene75508	0.828631	8.07E-55	-1.69E-46
MS.gene058109	MS.gene75508	0.819265	1.27E-52	-1.69E-46
MS.gene058111	MS.gene75508	0.857785	1.29E-62	-1.69E-46
MS.gene058171	MS.gene75508	0.825798	3.85E-54	-1.69E-46
MS.gene058173	MS.gene75508	0.819547	1.10E-52	-1.69E-46
MS.gene058175	MS.gene75508	0.826426	2.73E-54	-1.69E-46
MS.gene058268	MS.gene75508	0.814733	1.33E-51	-1.69E-46
MS.gene058269	MS.gene75508	0.805568	1.25E-49	-1.69E-46
MS.gene058493	MS.gene75508	0.867335	1.48E-65	-1.69E-46
MS.gene058494	MS.gene75508	0.843907	1.05E-58	-1.69E-46
MS.gene058628	MS.gene75508	0.867156	1.68E-65	-1.69E-46
MS.gene058629	MS.gene75508	0.858476	8.03E-63	-1.69E-46
MS.gene058630	MS.gene75508	0.871346	7.33E-67	-1.69E-46
MS.gene059045	MS.gene75508	0.814832	1.26E-51	-1.69E-46
MS.gene059690	MS.gene75508	0.837307	5.61E-57	-1.69E-46
MS.gene059694	MS.gene75508	0.837411	5.28E-57	-1.69E-46
MS.gene059697	MS.gene75508	0.828574	8.34E-55	-1.69E-46
MS.gene059865	MS.gene75508	-0.82096	5.20E-53	-1.69E-46
MS.gene060595	MS.gene75508	0.831997	1.22E-55	-1.69E-46
MS.gene06077	MS.gene75508	-0.817274	3.59E-52	-1.69E-46
MS.gene060982	MS.gene75508	-0.804121	2.51E-49	-1.69E-46
MS.gene061044	MS.gene75508	0.800269	1.56E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75508.t1	MTR_3g086830	94.156	154	9	0	1	154	1	154	1.32e-81	238
MS.gene75508.t1	MTR_8g089920	43.137	153	75	4	4	154	1	143	6.05e-33	114

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 39 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTGGTAATTGTTTATCATT+TGG	0.298328	3.3:-72411561	None:intergenic
AGGAATCTGTAGTGAATTCT+AGG	0.331571	3.3:+72411940	MS.gene75508:CDS
AGGGATGTTGGTGGAGGTAG+AGG	0.333746	3.3:+72411861	MS.gene75508:CDS
GAAGAAAGAAAGATGGCTTT+TGG	0.373560	3.3:+72411883	MS.gene75508:CDS
TTGTTTCGAGGAACCGAGTC+AGG	0.374583	3.3:+72411835	MS.gene75508:CDS
AGGTACTCAAATTGAGGAAA+AGG	0.393008	3.3:+72411910	MS.gene75508:CDS
GAGAGAATCAATAGGGTTCT+TGG	0.399060	3.3:-72411628	None:intergenic
TGAGGAGAAGCAGGAGAAGA+AGG	0.411004	3.3:-72411663	None:intergenic
CAATAGTAGAGAGAATCAAT+AGG	0.415959	3.3:-72411636	None:intergenic
TTTGTTGAGGCATCATCAAT+AGG	0.416519	3.3:-72411687	None:intergenic
GATGATGCCTCAACAAATCA+TGG	0.439486	3.3:+72411693	MS.gene75508:CDS
TAGTGAATTCTAGGACTTGA+AGG	0.449666	3.3:+72411949	MS.gene75508:CDS
GGTCCAAGGTACTCAAATTG+AGG	0.456858	3.3:+72411904	MS.gene75508:CDS
TTTCCTCAATTTGAGTACCT+TGG	0.458595	3.3:-72411907	None:intergenic
AGGGAGAGGGATGTTGGTGG+AGG	0.478556	3.3:+72411855	MS.gene75508:CDS
AGTCGCTTGCCGTTGTTTCG+AGG	0.489139	3.3:+72411823	MS.gene75508:CDS
TCAATAGGTTGAGGAGAAGC+AGG	0.490038	3.3:-72411672	None:intergenic
GGTACTCAAATTGAGGAAAA+GGG	0.507988	3.3:+72411911	MS.gene75508:CDS
TGTCATACAAGAAGCAGAAC+CGG	0.515419	3.3:-72411766	None:intergenic
AATTAGAAGCAACAAGAGTA+AGG	0.516499	3.3:+72411526	MS.gene75508:CDS
GGTAGAGGAAGAAAGAAAGA+TGG	0.520786	3.3:+72411876	MS.gene75508:CDS
CGAGGAACCGAGTCAGGGAG+AGG	0.521049	3.3:+72411841	MS.gene75508:CDS
AATAGTAGAGAGAATCAATA+GGG	0.528508	3.3:-72411635	None:intergenic
CAACATCCCTCTCCCTGACT+CGG	0.533206	3.3:-72411848	None:intergenic
CGAGTCAGGGAGAGGGATGT+TGG	0.536071	3.3:+72411849	MS.gene75508:CDS
CAATAATGAATCAGATGAAG+TGG	0.549086	3.3:+72411499	None:intergenic
GTCAGGGAGAGGGATGTTGG+TGG	0.559065	3.3:+72411852	MS.gene75508:CDS
GAGGCATCATCAATAGGTTG+AGG	0.568712	3.3:-72411681	None:intergenic
GAGTTTGTCTCTTAAACAAG+AGG	0.578724	3.3:-72411592	None:intergenic
CCAAATGATAAACAATTACC+AGG	0.580717	3.3:+72411561	MS.gene75508:CDS
GAGGAACCGAGTCAGGGAGA+GGG	0.581827	3.3:+72411842	MS.gene75508:CDS
ATCGTAGTAGACACTTTCGC+AGG	0.593355	3.3:+72411727	MS.gene75508:CDS
TGAAGACCCATGATTTGTTG+AGG	0.607126	3.3:-72411700	None:intergenic
AGGAATGCTTCACAAGCGAC+CGG	0.610619	3.3:+72411747	MS.gene75508:CDS
ATGATGCCTCAACAAATCAT+GGG	0.612329	3.3:+72411694	MS.gene75508:CDS
ATTGAGGAAAAGGGATACAG+AGG	0.620071	3.3:+72411920	MS.gene75508:CDS
TGTTTCGAGGAACCGAGTCA+GGG	0.624425	3.3:+72411836	MS.gene75508:CDS
AAACAAGAGGAACAAACACC+TGG	0.650993	3.3:-72411579	None:intergenic
GACTCGGTTCCTCGAAACAA+CGG	0.676399	3.3:-72411832	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATAGTAGAGAGAATCAATA+GGG	-	chr3.3:72411638-72411657	None:intergenic	25.0%
	AATTAGAAGCAACAAGAGTA+AGG	+	chr3.3:72411526-72411545	MS.gene75508:CDS	30.0%
	CAATAGTAGAGAGAATCAAT+AGG	-	chr3.3:72411639-72411658	None:intergenic	30.0%
	CCAAATGATAAACAATTACC+AGG	+	chr3.3:72411561-72411580	MS.gene75508:CDS	30.0%
	CCTGGTAATTGTTTATCATT+TGG	-	chr3.3:72411564-72411583	None:intergenic	30.0%
	AGGAATCTGTAGTGAATTCT+AGG	+	chr3.3:72411940-72411959	MS.gene75508:CDS	35.0%
	AGGTACTCAAATTGAGGAAA+AGG	+	chr3.3:72411910-72411929	MS.gene75508:CDS	35.0%
	ATGATGCCTCAACAAATCAT+GGG	+	chr3.3:72411694-72411713	MS.gene75508:CDS	35.0%
	GAGTTTGTCTCTTAAACAAG+AGG	-	chr3.3:72411595-72411614	None:intergenic	35.0%
	GGTACTCAAATTGAGGAAAA+GGG	+	chr3.3:72411911-72411930	MS.gene75508:CDS	35.0%
	TTTCCTCAATTTGAGTACCT+TGG	-	chr3.3:72411910-72411929	None:intergenic	35.0%
!	GAAGAAAGAAAGATGGCTTT+TGG	+	chr3.3:72411883-72411902	MS.gene75508:CDS	35.0%
!	TTTGTTGAGGCATCATCAAT+AGG	-	chr3.3:72411690-72411709	None:intergenic	35.0%
	AAACAAGAGGAACAAACACC+TGG	-	chr3.3:72411582-72411601	None:intergenic	40.0%
	ATTGAGGAAAAGGGATACAG+AGG	+	chr3.3:72411920-72411939	MS.gene75508:CDS	40.0%
	GAGAGAATCAATAGGGTTCT+TGG	-	chr3.3:72411631-72411650	None:intergenic	40.0%
	GATGATGCCTCAACAAATCA+TGG	+	chr3.3:72411693-72411712	MS.gene75508:CDS	40.0%
	GGTAGAGGAAGAAAGAAAGA+TGG	+	chr3.3:72411876-72411895	MS.gene75508:CDS	40.0%
	TGTCATACAAGAAGCAGAAC+CGG	-	chr3.3:72411769-72411788	None:intergenic	40.0%
!!	TGAAGACCCATGATTTGTTG+AGG	-	chr3.3:72411703-72411722	None:intergenic	40.0%
	ATCGTAGTAGACACTTTCGC+AGG	+	chr3.3:72411727-72411746	MS.gene75508:CDS	45.0%
!	GAAAGATGGCTTTTGGTCCA+AGG	+	chr3.3:72411890-72411909	MS.gene75508:CDS	45.0%
!	GAGGCATCATCAATAGGTTG+AGG	-	chr3.3:72411684-72411703	None:intergenic	45.0%
!	GGTCCAAGGTACTCAAATTG+AGG	+	chr3.3:72411904-72411923	MS.gene75508:CDS	45.0%
!	TCAATAGGTTGAGGAGAAGC+AGG	-	chr3.3:72411675-72411694	None:intergenic	45.0%
	AGGAATGCTTCACAAGCGAC+CGG	+	chr3.3:72411747-72411766	MS.gene75508:CDS	50.0%
	TGAGGAGAAGCAGGAGAAGA+AGG	-	chr3.3:72411666-72411685	None:intergenic	50.0%
	TGTTTCGAGGAACCGAGTCA+GGG	+	chr3.3:72411836-72411855	MS.gene75508:CDS	50.0%
	TTGTTTCGAGGAACCGAGTC+AGG	+	chr3.3:72411835-72411854	MS.gene75508:CDS	50.0%
!	GACTCGGTTCCTCGAAACAA+CGG	-	chr3.3:72411835-72411854	None:intergenic	50.0%
	CAACATCCCTCTCCCTGACT+CGG	-	chr3.3:72411851-72411870	None:intergenic	55.0%
!	AGGGATGTTGGTGGAGGTAG+AGG	+	chr3.3:72411861-72411880	MS.gene75508:CDS	55.0%
!	AGTCGCTTGCCGTTGTTTCG+AGG	+	chr3.3:72411823-72411842	MS.gene75508:CDS	55.0%
	CGAGTCAGGGAGAGGGATGT+TGG	+	chr3.3:72411849-72411868	MS.gene75508:CDS	60.0%
	GAGGAACCGAGTCAGGGAGA+GGG	+	chr3.3:72411842-72411861	MS.gene75508:CDS	60.0%
	GTCAGGGAGAGGGATGTTGG+TGG	+	chr3.3:72411852-72411871	MS.gene75508:CDS	60.0%
!	AGGGAGAGGGATGTTGGTGG+AGG	+	chr3.3:72411855-72411874	MS.gene75508:CDS	60.0%
	CGAGGAACCGAGTCAGGGAG+AGG	+	chr3.3:72411841-72411860	MS.gene75508:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	72411504	72411968	72411504	ID=MS.gene75508
chr3.3	mRNA	72411504	72411968	72411504	ID=MS.gene75508.t1;Parent=MS.gene75508
chr3.3	exon	72411504	72411968	72411504	ID=MS.gene75508.t1.exon1;Parent=MS.gene75508.t1
chr3.3	CDS	72411504	72411968	72411504	ID=cds.MS.gene75508.t1;Parent=MS.gene75508.t1

Gene Sequence

>MS.gene75508

ATGAATCAGATGAAGTGGTGCGAATTAGAAGCAACAAGAGTAAGGTGCAAAGAACATCCAAATGATAAACAATTACCAGGTGTTTGTTCCTCTTGTTTAAGAGACAAACTCTCAAATCTATACACCAAGAACCCTATTGATTCTCTCTACTATTGTTCTCCTTCTTCTCCTGCTTCTCCTCAACCTATTGATGATGCCTCAACAAATCATGGGTCTTCAGCTCATCGTAGTAGACACTTTCGCAGGAATGCTTCACAAGCGACCGGTTCTGCTTCTTGTATGACAAGCTTTAATCATGCGTTGAACTTGAAGAAGAGCAAGTCGCTTGCCGTTGTTTCGAGGAACCGAGTCAGGGAGAGGGATGTTGGTGGAGGTAGAGGAAGAAAGAAAGATGGCTTTTGGTCCAAGGTACTCAAATTGAGGAAAAGGGATACAGAGGAATCTGTAGTGAATTCTAGGACTTGA

Protein sequence

>MS.gene75508.t1

MNQMKWCELEATRVRCKEHPNDKQLPGVCSSCLRDKLSNLYTKNPIDSLYYCSPSSPASPQPIDDASTNHGSSAHRSRHFRRNASQATGSASCMTSFNHALNLKKSKSLAVVSRNRVRERDVGGGRGRKKDGFWSKVLKLRKRDTEESVVNSRT