AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76347


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76347.t1 MTR_8g078100 98.667 375 5 0 1 375 1 375 0.0 760
MS.gene76347.t1 MTR_5g019430 70.822 377 105 2 1 372 1 377 0.0 546
MS.gene76347.t1 MTR_4g069160 57.825 377 153 4 1 375 1 373 2.44e-146 419
MS.gene76347.t1 MTR_1g034480 55.467 375 160 3 1 374 1 369 1.02e-137 397
MS.gene76347.t1 MTR_3g011140 63.514 74 27 0 285 358 1 74 9.94e-27 102
MS.gene76347.t1 MTR_8g068520 22.293 314 206 7 76 373 86 377 5.42e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76347.t1 AT2G18630 54.090 379 168 2 1 374 2 379 1.32e-138 400
MS.gene76347.t1 AT2G18630 54.090 379 168 2 1 374 2 379 1.59e-138 400
MS.gene76347.t1 AT4G34320 55.319 376 159 5 1 375 1 368 7.86e-133 385
MS.gene76347.t1 AT4G34320 55.319 376 159 5 1 375 1 368 7.86e-133 385
MS.gene76347.t1 AT5G66675 53.406 367 161 4 16 374 32 396 2.56e-128 375
MS.gene76347.t1 AT5G66675 53.406 367 161 4 16 374 32 396 2.56e-128 375
MS.gene76347.t1 AT5G66675 53.406 367 161 4 16 374 32 396 2.56e-128 375
MS.gene76347.t1 AT5G66675 53.406 367 161 4 16 374 32 396 2.56e-128 375
MS.gene76347.t1 AT4G34330 48.851 348 162 3 24 371 17 348 2.92e-107 319
MS.gene76347.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.50e-89 275
MS.gene76347.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.50e-89 275
MS.gene76347.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.50e-89 275
MS.gene76347.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.50e-89 275
MS.gene76347.t1 AT5G66660 41.713 362 203 2 21 374 35 396 5.80e-86 266
MS.gene76347.t1 AT3G28270 27.095 358 245 7 28 370 16 372 6.08e-35 132
MS.gene76347.t1 AT3G28270 27.095 358 245 7 28 370 16 372 6.08e-35 132
MS.gene76347.t1 AT3G28290 24.462 372 254 8 24 370 11 380 9.84e-30 118
MS.gene76347.t1 AT3G28300 24.462 372 254 8 24 370 11 380 9.84e-30 118
MS.gene76347.t1 AT3G57100 25.705 319 210 5 65 370 43 347 1.50e-25 106
MS.gene76347.t1 AT3G28320 24.444 315 211 8 80 370 2 313 3.76e-20 90.5

Find 50 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTTGTTAAAGTTATTT+TGG 0.163128 8.1:+29572805 MS.gene76347:CDS
TCATCAAAACTTTGCAAGTT+TGG 0.189922 8.1:-29572665 None:intergenic
GCTGCGTTGGTTGTTCCATT+AGG 0.253208 8.1:+29573301 MS.gene76347:CDS
TCGGGTGCTTACAAACAAAT+TGG 0.255573 8.1:+29573447 MS.gene76347:CDS
TGGATTGCAAGAGGGATATA+TGG 0.257789 8.1:+29572825 MS.gene76347:CDS
AAATAAAACTCCTCTGTAAA+AGG 0.261071 8.1:-29573073 None:intergenic
TTTGAAGAAGAGGTGCAAAA+TGG 0.287245 8.1:+29572986 MS.gene76347:CDS
TTACATTTCATTGAAGGATT+TGG 0.290195 8.1:+29573417 MS.gene76347:CDS
TTGCTACTGGTGTTGAAGTT+AGG 0.293911 8.1:+29572726 MS.gene76347:CDS
CTGCGTTGGTTGTTCCATTA+GGG 0.303758 8.1:+29573302 MS.gene76347:CDS
CATTAGGGTCAGTTGGAAAA+TGG 0.326311 8.1:+29573317 MS.gene76347:CDS
GGGTTATGAGAAGGCTATAA+AGG 0.331087 8.1:+29573357 MS.gene76347:CDS
TGAAGGATTTGGACAACATT+CGG 0.353812 8.1:+29573428 MS.gene76347:CDS
GTTATTAGTTCTCTTGCTAC+TGG 0.384834 8.1:+29572713 MS.gene76347:CDS
GAAGGATTTGGACAACATTC+GGG 0.389768 8.1:+29573429 MS.gene76347:CDS
GCAGCCAAAGCTGTTACAAC+TGG 0.412158 8.1:-29573280 None:intergenic
GGATTGCAAGAGGGATATAT+GGG 0.412828 8.1:+29572826 MS.gene76347:CDS
TCAAAGACCTCATCTTAATC+AGG 0.418187 8.1:-29573670 None:intergenic
TCCGCCAGTTGTAACAGCTT+TGG 0.428937 8.1:+29573276 MS.gene76347:CDS
GAGGTGCAAAATGGGCTTGA+AGG 0.435928 8.1:+29572995 MS.gene76347:CDS
TTGAAGAAGAGGTGCAAAAT+GGG 0.439323 8.1:+29572987 MS.gene76347:CDS
AAGAGAACTAATAACCCTAT+TGG 0.447555 8.1:-29572705 None:intergenic
GGTTATGAGAAGGCTATAAA+GGG 0.448159 8.1:+29573358 MS.gene76347:CDS
GTATTAAAGAGCATACCAAT+AGG 0.457807 8.1:+29572690 MS.gene76347:CDS
TGGTACTTACATTTCATTGA+AGG 0.469068 8.1:+29573411 MS.gene76347:CDS
AACAGCTTTGGCTGCTGCGT+TGG 0.469212 8.1:+29573288 MS.gene76347:CDS
AATTACAAAACTCCAACGTC+TGG 0.474086 8.1:-29572898 None:intergenic
AGGTGCAAAATGGGCTTGAA+GGG 0.480313 8.1:+29572996 MS.gene76347:CDS
CTCTTGAGAAATGTTTGAGA+CGG 0.491708 8.1:+29572921 MS.gene76347:CDS
TGAAGACATTGCAAGAGCTT+AGG 0.500716 8.1:+29573029 MS.gene76347:CDS
AGGAAATTCAAGCAAGATGA+TGG 0.501543 8.1:+29572556 MS.gene76347:CDS
GTTGTTCCATTAGGGTCAGT+TGG 0.502956 8.1:+29573310 MS.gene76347:CDS
TCAATCGGTTTATACGCAGC+AGG 0.509528 8.1:+29573102 MS.gene76347:CDS
TATTAAAGAGCATACCAATA+GGG 0.511363 8.1:+29572691 MS.gene76347:CDS
TCTTGAGAAATGTTTGAGAC+GGG 0.517157 8.1:+29572922 MS.gene76347:CDS
AAACAAGGATTTGTTCGCGT+TGG 0.546843 8.1:+29572850 MS.gene76347:CDS
AATTGAAATCTCTCAAGTCA+TGG 0.554098 8.1:+29573173 MS.gene76347:CDS
GAATCAAGTGATTGTTAAGT+CGG 0.557993 8.1:+29572952 MS.gene76347:CDS
AATCTCTCAAGTCATGGAAG+AGG 0.565998 8.1:+29573179 MS.gene76347:CDS
GCCAAAGCTGTTACAACTGG+CGG 0.571434 8.1:-29573277 None:intergenic
GATTTGCAATTTCTTCAACA+TGG 0.599579 8.1:-29573128 None:intergenic
ATCTCTCAAGTCATGGAAGA+GGG 0.600067 8.1:+29573180 MS.gene76347:CDS
TGATAATAGTCTCCAGACGT+TGG 0.605979 8.1:+29572886 MS.gene76347:CDS
GAGGGATATATGGGGAAACA+AGG 0.609168 8.1:+29572835 MS.gene76347:CDS
GGTGCAAAATGGGCTTGAAG+GGG 0.611042 8.1:+29572997 MS.gene76347:CDS
GTAGTTAGCTCAATGCAGCT+TGG 0.613218 8.1:+29573391 MS.gene76347:CDS
ATCTGAGGTATTAAGATGGG+AGG 0.616456 8.1:+29572536 None:intergenic
GTGCAAAATGGGCTTGAAGG+GGG 0.641754 8.1:+29572998 MS.gene76347:CDS
GATTGCAAGAGGGATATATG+GGG 0.687000 8.1:+29572827 MS.gene76347:CDS
GTCGACAGATAAGAAAAGCG+AGG 0.701787 8.1:+29573617 MS.gene76347:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTATTTGTTGTTTCAAT+CGG + chr8.1:29573087-29573106 MS.gene76347:CDS 20.0%
!!! TGAAGTTGTTAAAGTTATTT+TGG + chr8.1:29572805-29572824 MS.gene76347:CDS 20.0%
! AAATAAAACTCCTCTGTAAA+AGG - chr8.1:29573076-29573095 None:intergenic 25.0%
! TTACATTTCATTGAAGGATT+TGG + chr8.1:29573417-29573436 MS.gene76347:CDS 25.0%
!! TATTAAAGAGCATACCAATA+GGG + chr8.1:29572691-29572710 MS.gene76347:CDS 25.0%
!! TGATTTTGCTCTTAAAAATG+AGG + chr8.1:29573501-29573520 MS.gene76347:CDS 25.0%
!!! GATTACTTATTTTGAAGAAG+AGG + chr8.1:29572976-29572995 MS.gene76347:CDS 25.0%
AAGAGAACTAATAACCCTAT+TGG - chr8.1:29572708-29572727 None:intergenic 30.0%
AATTGAAATCTCTCAAGTCA+TGG + chr8.1:29573173-29573192 MS.gene76347:CDS 30.0%
GAATCAAGTGATTGTTAAGT+CGG + chr8.1:29572952-29572971 MS.gene76347:CDS 30.0%
GATTTGCAATTTCTTCAACA+TGG - chr8.1:29573131-29573150 None:intergenic 30.0%
GCTAATGATTGCACAAAAAT+GGG + chr8.1:29572584-29572603 MS.gene76347:CDS 30.0%
TCATCAAAACTTTGCAAGTT+TGG - chr8.1:29572668-29572687 None:intergenic 30.0%
TGCTAATGATTGCACAAAAA+TGG + chr8.1:29572583-29572602 MS.gene76347:CDS 30.0%
TGGTACTTACATTTCATTGA+AGG + chr8.1:29573411-29573430 MS.gene76347:CDS 30.0%
! GTATTAAAGAGCATACCAAT+AGG + chr8.1:29572690-29572709 MS.gene76347:CDS 30.0%
!! ACTTTTTAAGGGTTATGAGA+AGG + chr8.1:29573348-29573367 MS.gene76347:CDS 30.0%
!! TTAGGAATTTTAAGGATGCT+GGG + chr8.1:29573047-29573066 MS.gene76347:CDS 30.0%
!!! AAGTTATTTTGGATTGCAAG+AGG + chr8.1:29572816-29572835 MS.gene76347:CDS 30.0%
!!! AGAAACTTTTTCAGAGACAT+TGG + chr8.1:29573564-29573583 MS.gene76347:CDS 30.0%
!!! AGTTATTTTGGATTGCAAGA+GGG + chr8.1:29572817-29572836 MS.gene76347:CDS 30.0%
!!! ATGGTGTAACTCACTTTTTA+AGG + chr8.1:29573336-29573355 MS.gene76347:CDS 30.0%
!!! TGGTGTAACTCACTTTTTAA+GGG + chr8.1:29573337-29573356 MS.gene76347:CDS 30.0%
!!! TTTTTTGGCTGCTTTTGTTT+CGG + chr8.1:29573216-29573235 MS.gene76347:CDS 30.0%
AATTACAAAACTCCAACGTC+TGG - chr8.1:29572901-29572920 None:intergenic 35.0%
AGGAAATTCAAGCAAGATGA+TGG + chr8.1:29572556-29572575 MS.gene76347:CDS 35.0%
CTCTTGAGAAATGTTTGAGA+CGG + chr8.1:29572921-29572940 MS.gene76347:CDS 35.0%
GGTTATGAGAAGGCTATAAA+GGG + chr8.1:29573358-29573377 MS.gene76347:CDS 35.0%
TGAAGGATTTGGACAACATT+CGG + chr8.1:29573428-29573447 MS.gene76347:CDS 35.0%
! GCAAGAGCTTAGGAATTTTA+AGG + chr8.1:29573039-29573058 MS.gene76347:CDS 35.0%
! GTTATTAGTTCTCTTGCTAC+TGG + chr8.1:29572713-29572732 MS.gene76347:CDS 35.0%
! TCTTGAGAAATGTTTGAGAC+GGG + chr8.1:29572922-29572941 MS.gene76347:CDS 35.0%
! TTGAAGAAGAGGTGCAAAAT+GGG + chr8.1:29572987-29573006 MS.gene76347:CDS 35.0%
! TTTGAAGAAGAGGTGCAAAA+TGG + chr8.1:29572986-29573005 MS.gene76347:CDS 35.0%
!! CTTAGGAATTTTAAGGATGC+TGG + chr8.1:29573046-29573065 MS.gene76347:CDS 35.0%
!! TAGGAATTTTAAGGATGCTG+GGG + chr8.1:29573048-29573067 MS.gene76347:CDS 35.0%
!!! GGTGTCGAATGCAATTTTTT+TGG + chr8.1:29573201-29573220 MS.gene76347:CDS 35.0%
AATCTCTCAAGTCATGGAAG+AGG + chr8.1:29573179-29573198 MS.gene76347:CDS 40.0%
ATCTCTCAAGTCATGGAAGA+GGG + chr8.1:29573180-29573199 MS.gene76347:CDS 40.0%
CATTAGGGTCAGTTGGAAAA+TGG + chr8.1:29573317-29573336 MS.gene76347:CDS 40.0%
GAAGGATTTGGACAACATTC+GGG + chr8.1:29573429-29573448 MS.gene76347:CDS 40.0%
GATTGCAAGAGGGATATATG+GGG + chr8.1:29572827-29572846 MS.gene76347:CDS 40.0%
GGATTGCAAGAGGGATATAT+GGG + chr8.1:29572826-29572845 MS.gene76347:CDS 40.0%
GGGTTATGAGAAGGCTATAA+AGG + chr8.1:29573357-29573376 MS.gene76347:CDS 40.0%
TCGGGTGCTTACAAACAAAT+TGG + chr8.1:29573447-29573466 MS.gene76347:CDS 40.0%
TGAAGACATTGCAAGAGCTT+AGG + chr8.1:29573029-29573048 MS.gene76347:CDS 40.0%
TGATAATAGTCTCCAGACGT+TGG + chr8.1:29572886-29572905 MS.gene76347:CDS 40.0%
TGGATTGCAAGAGGGATATA+TGG + chr8.1:29572825-29572844 MS.gene76347:CDS 40.0%
! AAACAAGGATTTGTTCGCGT+TGG + chr8.1:29572850-29572869 MS.gene76347:CDS 40.0%
! CATTTTCCAACTGACCCTAA+TGG - chr8.1:29573319-29573338 None:intergenic 40.0%
! TTGCTACTGGTGTTGAAGTT+AGG + chr8.1:29572726-29572745 MS.gene76347:CDS 40.0%
!!! TTCGGTGTTGATTTTCTCTG+TGG + chr8.1:29573234-29573253 MS.gene76347:CDS 40.0%
GAGGGATATATGGGGAAACA+AGG + chr8.1:29572835-29572854 MS.gene76347:CDS 45.0%
GTAGTTAGCTCAATGCAGCT+TGG + chr8.1:29573391-29573410 MS.gene76347:CDS 45.0%
GTCGACAGATAAGAAAAGCG+AGG + chr8.1:29573617-29573636 MS.gene76347:CDS 45.0%
GTTGTTCCATTAGGGTCAGT+TGG + chr8.1:29573310-29573329 MS.gene76347:CDS 45.0%
! AGGTGCAAAATGGGCTTGAA+GGG + chr8.1:29572996-29573015 MS.gene76347:CDS 45.0%
! TCAATCGGTTTATACGCAGC+AGG + chr8.1:29573102-29573121 MS.gene76347:CDS 45.0%
!! CTGCGTTGGTTGTTCCATTA+GGG + chr8.1:29573302-29573321 MS.gene76347:CDS 45.0%
GCAGCCAAAGCTGTTACAAC+TGG - chr8.1:29573283-29573302 None:intergenic 50.0%
GCCAAAGCTGTTACAACTGG+CGG - chr8.1:29573280-29573299 None:intergenic 50.0%
TCCGCCAGTTGTAACAGCTT+TGG + chr8.1:29573276-29573295 MS.gene76347:CDS 50.0%
! GAGGTGCAAAATGGGCTTGA+AGG + chr8.1:29572995-29573014 MS.gene76347:CDS 50.0%
! GGTGCAAAATGGGCTTGAAG+GGG + chr8.1:29572997-29573016 MS.gene76347:CDS 50.0%
! GTGCAAAATGGGCTTGAAGG+GGG + chr8.1:29572998-29573017 MS.gene76347:CDS 50.0%
! TGCTGGGGATCCTTTTACAG+AGG + chr8.1:29573063-29573082 MS.gene76347:CDS 50.0%
!! GCTGCGTTGGTTGTTCCATT+AGG + chr8.1:29573301-29573320 MS.gene76347:CDS 50.0%
! AACAGCTTTGGCTGCTGCGT+TGG + chr8.1:29573288-29573307 MS.gene76347:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 29572551 29573678 29572551 ID=MS.gene76347
chr8.1 mRNA 29572551 29573678 29572551 ID=MS.gene76347.t1;Parent=MS.gene76347
chr8.1 exon 29572551 29573678 29572551 ID=MS.gene76347.t1.exon1;Parent=MS.gene76347.t1
chr8.1 CDS 29572551 29573678 29572551 ID=cds.MS.gene76347.t1;Parent=MS.gene76347.t1
Gene Sequence

>MS.gene76347

ATGGGAGGAAATTCAAGCAAGATGATGGTTGATGCTAATGATTGCACAAAAATGGGTGCTCATTCTGTGTATGCAGCTGATTTAAGCTCATATGAAGCTGCATGTGTTAAAGATCCAAACTTGCAAAGTTTTGATGAGAGTATTAAAGAGCATACCAATAGGGTTATTAGTTCTCTTGCTACTGGTGTTGAAGTTAGGTCTTTATCTTTCGATTCGCTGCGAGAAGTTACTGATAGTTTGCTTGAGATGAATCATGAAGTTGTTAAAGTTATTTTGGATTGCAAGAGGGATATATGGGGAAACAAGGATTTGTTCGCGTTGGTTAATGATTATTTTGATAATAGTCTCCAGACGTTGGAGTTTTGTAATTCTCTTGAGAAATGTTTGAGACGGGCACGCGAGAATCAAGTGATTGTTAAGTCGGTGATTACTTATTTTGAAGAAGAGGTGCAAAATGGGCTTGAAGGGGGAACTTGTGTGAAGACATTGCAAGAGCTTAGGAATTTTAAGGATGCTGGGGATCCTTTTACAGAGGAGTTTTATTTGTTGTTTCAATCGGTTTATACGCAGCAGGCAACCATGTTGAAGAAATTGCAAATCAGAAAGAGAAAGCTTGATAAGAAATTGAAATCTCTCAAGTCATGGAAGAGGGTGTCGAATGCAATTTTTTTGGCTGCTTTTGTTTCGGTGTTGATTTTCTCTGTGGTTGCAGCTTCTATTGCTGCTCCGCCAGTTGTAACAGCTTTGGCTGCTGCGTTGGTTGTTCCATTAGGGTCAGTTGGAAAATGGTGTAACTCACTTTTTAAGGGTTATGAGAAGGCTATAAAGGGACAAAGAGAAGTAGTTAGCTCAATGCAGCTTGGTACTTACATTTCATTGAAGGATTTGGACAACATTCGGGTGCTTACAAACAAATTGGAACTACAGCTTGAATCGTTAATGCAGAATGCTGATTTTGCTCTTAAAAATGAGGATGTCATAAAGCTTGCAATCGATGAGATTAAGAAGAATATAGAAACTTTTTCAGAGACATTGGAAACTCTAAGTGCAAATGCTGATAAATGCAGTCGACAGATAAGAAAAGCGAGGACAGTCGTTATACAAAATATAATCAAAAGACCTGATTAA

Protein sequence

>MS.gene76347.t1

MGGNSSKMMVDANDCTKMGAHSVYAADLSSYEAACVKDPNLQSFDESIKEHTNRVISSLATGVEVRSLSFDSLREVTDSLLEMNHEVVKVILDCKRDIWGNKDLFALVNDYFDNSLQTLEFCNSLEKCLRRARENQVIVKSVITYFEEEVQNGLEGGTCVKTLQELRNFKDAGDPFTEEFYLLFQSVYTQQATMLKKLQIRKRKLDKKLKSLKSWKRVSNAIFLAAFVSVLIFSVVAASIAAPPVVTALAAALVVPLGSVGKWCNSLFKGYEKAIKGQREVVSSMQLGTYISLKDLDNIRVLTNKLELQLESLMQNADFALKNEDVIKLAIDEIKKNIETFSETLETLSANADKCSRQIRKARTVVIQNIIKRPD