AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77223


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77223.t1 MTR_7g058640 93.846 195 12 0 1 195 1 195 3.26e-138 385
MS.gene77223.t1 MTR_7g058640 95.930 172 7 0 1 172 1 172 1.19e-123 348
MS.gene77223.t1 MTR_4g022570 74.872 195 49 0 1 195 1 195 2.67e-111 318
MS.gene77223.t1 MTR_1g047720 66.197 213 72 0 1 213 1 213 4.68e-111 317
MS.gene77223.t1 MTR_7g058900 70.769 195 57 0 1 195 1 195 2.72e-104 300
MS.gene77223.t1 MTR_4g083490 67.358 193 63 0 3 195 5 197 5.98e-101 291
MS.gene77223.t1 MTR_2g436870 61.033 213 83 0 1 213 1 213 1.52e-100 290
MS.gene77223.t1 MTR_2g028790 65.803 193 66 0 3 195 7 199 2.25e-97 282
MS.gene77223.t1 MTR_1g111890 47.619 231 102 2 1 212 1 231 3.58e-80 239
MS.gene77223.t1 MTR_7g058640 94.017 117 7 0 79 195 1 117 6.84e-77 227
MS.gene77223.t1 MTR_1g047710 60.902 133 51 1 56 187 3 135 3.37e-57 177
MS.gene77223.t1 MTR_4g022470 71.028 107 31 0 64 170 10 116 1.50e-52 165
MS.gene77223.t1 MTR_5g089370 36.649 191 118 3 5 194 2 190 2.82e-38 132
MS.gene77223.t1 MTR_4g052550 32.461 191 126 3 5 194 2 190 1.45e-36 127
MS.gene77223.t1 MTR_4g115390 29.524 210 141 4 6 210 3 210 3.16e-29 108
MS.gene77223.t1 MTR_4g022550 71.053 38 11 0 158 195 10 47 1.82e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77223.t1 AT2G33120 72.821 195 53 0 1 195 1 195 7.80e-108 309
MS.gene77223.t1 AT1G04760 69.000 200 62 0 1 200 1 200 9.28e-108 309
MS.gene77223.t1 AT1G04760 69.000 200 62 0 1 200 1 200 9.28e-108 309
MS.gene77223.t1 AT1G04750 72.308 195 54 0 1 195 1 195 2.18e-107 308
MS.gene77223.t1 AT2G33120 69.458 203 54 1 1 195 1 203 1.24e-104 301
MS.gene77223.t1 AT2G32670 69.744 195 59 0 1 195 66 260 2.28e-104 303
MS.gene77223.t1 AT4G15780 61.202 183 70 1 1 182 1 183 2.99e-83 245
MS.gene77223.t1 AT2G33110 60.513 195 73 1 1 195 1 191 5.21e-83 246
MS.gene77223.t1 AT3G54300 48.069 233 98 2 1 212 1 231 2.73e-79 237
MS.gene77223.t1 AT3G54300 48.069 233 98 2 1 212 1 231 2.73e-79 237
MS.gene77223.t1 AT3G54300 48.069 233 98 2 1 212 1 231 2.73e-79 237
MS.gene77223.t1 AT2G33110 63.095 168 62 0 1 168 1 168 2.28e-76 228
MS.gene77223.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.39e-58 181
MS.gene77223.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.39e-58 181
MS.gene77223.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.39e-58 181
MS.gene77223.t1 AT5G11150 33.649 211 133 4 5 213 2 207 3.66e-39 134
MS.gene77223.t1 AT5G22360 32.536 209 136 4 5 211 2 207 4.25e-39 134
MS.gene77223.t1 AT5G11150 33.649 211 133 4 5 213 2 207 6.16e-39 134
MS.gene77223.t1 AT4G32150 34.211 190 122 3 5 193 2 189 8.70e-35 123
MS.gene77223.t1 AT2G25340 32.105 190 126 3 5 193 2 189 3.52e-32 116
MS.gene77223.t1 AT2G33120 67.742 62 20 0 134 195 52 113 4.65e-22 88.2
MS.gene77223.t1 AT3G24890 45.745 94 45 2 113 200 14 107 2.77e-18 77.8

Find 41 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCTTGAATAAAGAATT+TGG 0.138378 7.2:+37326886 MS.gene77223:CDS
CTTTGCAGGCACAAGATTTA+AGG 0.164312 7.2:+37327580 MS.gene77223:intron
GCAAGTTTGCTAACCTCTTC+TGG 0.229459 7.2:-37327053 None:intergenic
CAATCTTCACTTGACGATTA+AGG 0.242055 7.2:-37327228 None:intergenic
AGCTATTTCAGCAAAGTTTC+CGG 0.255031 7.2:-37326381 None:intergenic
TGCATATGCTCCTTCAATTT+CGG 0.281591 7.2:-37327014 None:intergenic
GGCGCGTGGCACGGTGATTC+TGG 0.320729 7.2:+37326340 MS.gene77223:CDS
TGGTTGCTGGGAGTCTTTGC+AGG 0.323885 7.2:-37326412 None:intergenic
AAAGCTTGAATAAAGAATTT+GGG 0.394970 7.2:+37326887 MS.gene77223:CDS
TCCGGTGAAGTTGGTGTGCT+CGG 0.407070 7.2:-37326363 None:intergenic
AACTGTCAACCTTCGTGATC+AGG 0.421098 7.2:+37327267 MS.gene77223:CDS
TTTGCAGGCACAAGATTTAA+GGG 0.423640 7.2:+37327581 MS.gene77223:intron
CAAGAAAGGCCATAGCAATA+TGG 0.430285 7.2:-37326801 None:intergenic
ATCACTTATATACAGCTTTG+TGG 0.443785 7.2:+37326319 MS.gene77223:CDS
TATGTTGGAAAATATTGATC+AGG 0.458760 7.2:+37327112 MS.gene77223:CDS
CTCCTTGATGTGATCAAGAA+AGG 0.487927 7.2:-37326815 None:intergenic
AGCTTACTGCCTTGTTGCTG+TGG 0.489435 7.2:+37326765 MS.gene77223:intron
TCAAGTTCAAGATGTTATGT+TGG 0.490196 7.2:+37327097 MS.gene77223:CDS
AGCAAAGTTTCCGGTGAAGT+TGG 0.500993 7.2:-37326372 None:intergenic
CAGCTTTGTGGCGCGTGGCA+CGG 0.506415 7.2:+37326331 MS.gene77223:CDS
GTAATTTAGACCGAAATTGA+AGG 0.508592 7.2:+37327004 MS.gene77223:intron
GGCAAGTACTGAATCCATCA+TGG 0.518155 7.2:-37326484 None:intergenic
GCCGAGCACACCAACTTCAC+CGG 0.518367 7.2:+37326362 MS.gene77223:CDS
GGCCTTTCTTGATCACATCA+AGG 0.528346 7.2:+37326813 MS.gene77223:CDS
TTCAACTACCTCGCCCATGA+TGG 0.533919 7.2:+37326470 MS.gene77223:CDS
ATCATGGGCGAGGTAGTTGA+AGG 0.542753 7.2:-37326468 None:intergenic
ACTTGCGAAAGTAAAAGCTC+AGG 0.550493 7.2:+37327070 MS.gene77223:CDS
TACTGAATCCATCATGGGCG+AGG 0.571090 7.2:-37326478 None:intergenic
ATATACAGCTTTGTGGCGCG+TGG 0.589934 7.2:+37326326 MS.gene77223:CDS
TTTACCAAAAGATATGGTGG+AGG 0.594903 7.2:+37326841 MS.gene77223:CDS
TGCAGGCACAAGATTTAAGG+GGG 0.606935 7.2:+37327583 MS.gene77223:intron
TTGCAGGCACAAGATTTAAG+GGG 0.610788 7.2:+37327582 MS.gene77223:intron
GGCAATTAAGGAGAAAGATG+TGG 0.621585 7.2:+37327613 MS.gene77223:CDS
CAAAAGACTCCACAGCAACA+AGG 0.623781 7.2:-37326774 None:intergenic
GTAGTTGAAGGTATGACCAT+CGG 0.631667 7.2:-37326456 None:intergenic
GCAAGTACTGAATCCATCAT+GGG 0.632742 7.2:-37326483 None:intergenic
TTGTGTGGAACATCCAGAAG+AGG 0.637264 7.2:+37327040 MS.gene77223:CDS
AAATTCACTTACAACACCGA+TGG 0.653817 7.2:+37326440 MS.gene77223:CDS
CTTTCTTGATCACATCAAGG+AGG 0.668827 7.2:+37326816 MS.gene77223:CDS
GGAGCATATGCAATATTGTG+TGG 0.672159 7.2:+37327025 MS.gene77223:CDS
ATAAAAGGACCTGATCACGA+AGG 0.753478 7.2:-37327276 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATGTAATTTAGTTATTTA+TGG - chr7.2:37327476-37327495 None:intergenic 15.0%
!!! TTTTTTTTATTGTATATGCA+TGG + chr7.2:37326982-37327001 MS.gene77223:intron 15.0%
!! AAAAGCTTGAATAAAGAATT+TGG + chr7.2:37326886-37326905 MS.gene77223:CDS 20.0%
!! AAAGCTTGAATAAAGAATTT+GGG + chr7.2:37326887-37326906 MS.gene77223:CDS 20.0%
!! AGGTATATATATGTCTAAAT+TGG + chr7.2:37327132-37327151 MS.gene77223:intron 20.0%
!! TAAAAGAAAAAGATAAAACG+AGG - chr7.2:37326508-37326527 None:intergenic 20.0%
!! TTATAGTTATGTAACTGATA+TGG + chr7.2:37327546-37327565 MS.gene77223:intron 20.0%
!! TTTATGGATGAACAAATAAA+AGG - chr7.2:37327294-37327313 None:intergenic 20.0%
!!! TCTTTTACATAATCTAATTG+TGG + chr7.2:37326521-37326540 MS.gene77223:intron 20.0%
! CAAATCATAAATTTCCTTAG+AGG - chr7.2:37326674-37326693 None:intergenic 25.0%
! TATGTTGGAAAATATTGATC+AGG + chr7.2:37327112-37327131 MS.gene77223:CDS 25.0%
!! ATTTTTACACACACATGAAT+AGG + chr7.2:37327430-37327449 MS.gene77223:intron 25.0%
!! TTTTTACACACACATGAATA+GGG + chr7.2:37327431-37327450 MS.gene77223:intron 25.0%
!!! ATTTTATTTGTTTTGTCTGC+AGG + chr7.2:37327204-37327223 MS.gene77223:intron 25.0%
ATCACTTATATACAGCTTTG+TGG + chr7.2:37326319-37326338 MS.gene77223:CDS 30.0%
GTAATTTAGACCGAAATTGA+AGG + chr7.2:37327004-37327023 MS.gene77223:intron 30.0%
TAACTGATATGGTTTGAATG+TGG + chr7.2:37327557-37327576 MS.gene77223:intron 30.0%
TCAAATGCATTTACATGTAC+TGG + chr7.2:37326627-37326646 MS.gene77223:intron 30.0%
TCAAGTTCAAGATGTTATGT+TGG + chr7.2:37327097-37327116 MS.gene77223:CDS 30.0%
! GAGGATTTTACCAAAAGATA+TGG + chr7.2:37326835-37326854 MS.gene77223:CDS 30.0%
! GATTTTACCAAAAGATATGG+TGG + chr7.2:37326838-37326857 MS.gene77223:CDS 30.0%
! GTTGTAAGTGAATTTTGTGT+TGG - chr7.2:37326435-37326454 None:intergenic 30.0%
! TTTTACACACACATGAATAG+GGG + chr7.2:37327432-37327451 MS.gene77223:intron 30.0%
!! AGTGAAGATTGATGTTTTGA+TGG + chr7.2:37327240-37327259 MS.gene77223:CDS 30.0%
AAATTCACTTACAACACCGA+TGG + chr7.2:37326440-37326459 MS.gene77223:CDS 35.0%
CAATCTTCACTTGACGATTA+AGG - chr7.2:37327231-37327250 None:intergenic 35.0%
TATGTCTAAATTGGATCCTC+TGG + chr7.2:37327141-37327160 MS.gene77223:intron 35.0%
TCAATGCAATGCGATATATG+AGG + chr7.2:37326553-37326572 MS.gene77223:intron 35.0%
TGCATATGCTCCTTCAATTT+CGG - chr7.2:37327017-37327036 None:intergenic 35.0%
TTTACCAAAAGATATGGTGG+AGG + chr7.2:37326841-37326860 MS.gene77223:CDS 35.0%
TTTGCAGGCACAAGATTTAA+GGG + chr7.2:37327581-37327600 MS.gene77223:intron 35.0%
! AGCTATTTCAGCAAAGTTTC+CGG - chr7.2:37326384-37326403 None:intergenic 35.0%
! TTTTCCTCCACCATATCTTT+TGG - chr7.2:37326848-37326867 None:intergenic 35.0%
! TTTTGATCGCCATATTGCTA+TGG + chr7.2:37326792-37326811 MS.gene77223:CDS 35.0%
!! TACATGTACTGGTTTCATGT+CGG + chr7.2:37326638-37326657 MS.gene77223:intron 35.0%
!!! TGAATTTTGTGTTGGTTGCT+GGG - chr7.2:37326427-37326446 None:intergenic 35.0%
AACGATCTGTGCTTCAAAGT+CGG - chr7.2:37327169-37327188 None:intergenic 40.0%
AAGATTTAAGGGGGAATGCA+GGG + chr7.2:37327592-37327611 MS.gene77223:CDS 40.0%
ACTTGCGAAAGTAAAAGCTC+AGG + chr7.2:37327070-37327089 MS.gene77223:CDS 40.0%
ATAAAAGGACCTGATCACGA+AGG - chr7.2:37327279-37327298 None:intergenic 40.0%
CAAGAAAGGCCATAGCAATA+TGG - chr7.2:37326804-37326823 None:intergenic 40.0%
CTCCTTGATGTGATCAAGAA+AGG - chr7.2:37326818-37326837 None:intergenic 40.0%
CTTTCTTGATCACATCAAGG+AGG + chr7.2:37326816-37326835 MS.gene77223:CDS 40.0%
CTTTGCAGGCACAAGATTTA+AGG + chr7.2:37327580-37327599 MS.gene77223:intron 40.0%
GGAGCATATGCAATATTGTG+TGG + chr7.2:37327025-37327044 MS.gene77223:CDS 40.0%
GGCAATTAAGGAGAAAGATG+TGG + chr7.2:37327613-37327632 MS.gene77223:CDS 40.0%
TAGCTATGACGCATGGTTTA+TGG - chr7.2:37327310-37327329 None:intergenic 40.0%
TTGCAGGCACAAGATTTAAG+GGG + chr7.2:37327582-37327601 MS.gene77223:intron 40.0%
! CAGTACTATGTTTTCTGCGA+TGG - chr7.2:37327361-37327380 None:intergenic 40.0%
! GTAGTTGAAGGTATGACCAT+CGG - chr7.2:37326459-37326478 None:intergenic 40.0%
!! GCAAGTACTGAATCCATCAT+GGG - chr7.2:37326486-37326505 None:intergenic 40.0%
!!! GTGAATTTTGTGTTGGTTGC+TGG - chr7.2:37326428-37326447 None:intergenic 40.0%
AACTGTCAACCTTCGTGATC+AGG + chr7.2:37327267-37327286 MS.gene77223:CDS 45.0%
AGCAAAGTTTCCGGTGAAGT+TGG - chr7.2:37326375-37326394 None:intergenic 45.0%
CAAAAGACTCCACAGCAACA+AGG - chr7.2:37326777-37326796 None:intergenic 45.0%
CAAGATTTAAGGGGGAATGC+AGG + chr7.2:37327591-37327610 MS.gene77223:CDS 45.0%
GCAATGCGATATATGAGGCA+CGG + chr7.2:37326558-37326577 MS.gene77223:intron 45.0%
GGCCTTTCTTGATCACATCA+AGG + chr7.2:37326813-37326832 MS.gene77223:CDS 45.0%
TCATCACTAGCTATGACGCA+TGG - chr7.2:37327317-37327336 None:intergenic 45.0%
TCGGACATTGAACACCTCTA+AGG + chr7.2:37326657-37326676 MS.gene77223:intron 45.0%
TGCAGGCACAAGATTTAAGG+GGG + chr7.2:37327583-37327602 MS.gene77223:intron 45.0%
TTGTGTGGAACATCCAGAAG+AGG + chr7.2:37327040-37327059 MS.gene77223:CDS 45.0%
! TGGTTTGAATGTGGCTTTGC+AGG + chr7.2:37327566-37327585 MS.gene77223:intron 45.0%
!! GCAAGTTTGCTAACCTCTTC+TGG - chr7.2:37327056-37327075 None:intergenic 45.0%
!! GGCAAGTACTGAATCCATCA+TGG - chr7.2:37326487-37326506 None:intergenic 45.0%
ATCATGGGCGAGGTAGTTGA+AGG - chr7.2:37326471-37326490 None:intergenic 50.0%
TACTGAATCCATCATGGGCG+AGG - chr7.2:37326481-37326500 None:intergenic 50.0%
TTCAACTACCTCGCCCATGA+TGG + chr7.2:37326470-37326489 MS.gene77223:CDS 50.0%
! AGCTTACTGCCTTGTTGCTG+TGG + chr7.2:37326765-37326784 MS.gene77223:intron 50.0%
! ATATACAGCTTTGTGGCGCG+TGG + chr7.2:37326326-37326345 MS.gene77223:CDS 50.0%
GGGGGAATGCAGGGCAATTA+AGG + chr7.2:37327601-37327620 MS.gene77223:CDS 55.0%
TGGTTGCTGGGAGTCTTTGC+AGG - chr7.2:37326415-37326434 None:intergenic 55.0%
! TGCTTCAAAGTCGGCTCCAG+AGG - chr7.2:37327160-37327179 None:intergenic 55.0%
!! TCCGGTGAAGTTGGTGTGCT+CGG - chr7.2:37326366-37326385 None:intergenic 55.0%
GCCGAGCACACCAACTTCAC+CGG + chr7.2:37326362-37326381 MS.gene77223:CDS 60.0%
! CAGCTTTGTGGCGCGTGGCA+CGG + chr7.2:37326331-37326350 MS.gene77223:CDS 65.0%
GGCGCGTGGCACGGTGATTC+TGG + chr7.2:37326340-37326359 MS.gene77223:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 37326308 37327716 37326308 ID=MS.gene77223
chr7.2 mRNA 37326308 37327716 37326308 ID=MS.gene77223.t1;Parent=MS.gene77223
chr7.2 exon 37326308 37326497 37326308 ID=MS.gene77223.t1.exon1;Parent=MS.gene77223.t1
chr7.2 CDS 37326308 37326497 37326308 ID=cds.MS.gene77223.t1;Parent=MS.gene77223.t1
chr7.2 exon 37326767 37326908 37326767 ID=MS.gene77223.t1.exon2;Parent=MS.gene77223.t1
chr7.2 CDS 37326767 37326908 37326767 ID=cds.MS.gene77223.t1;Parent=MS.gene77223.t1
chr7.2 exon 37327013 37327133 37327013 ID=MS.gene77223.t1.exon3;Parent=MS.gene77223.t1
chr7.2 CDS 37327013 37327133 37327013 ID=cds.MS.gene77223.t1;Parent=MS.gene77223.t1
chr7.2 exon 37327226 37327288 37327226 ID=MS.gene77223.t1.exon4;Parent=MS.gene77223.t1
chr7.2 CDS 37327226 37327288 37327226 ID=cds.MS.gene77223.t1;Parent=MS.gene77223.t1
chr7.2 exon 37327588 37327716 37327588 ID=MS.gene77223.t1.exon5;Parent=MS.gene77223.t1
chr7.2 CDS 37327588 37327716 37327588 ID=cds.MS.gene77223.t1;Parent=MS.gene77223.t1
Gene Sequence

>MS.gene77223

ATGGCGCAACAATCACTTATATACAGCTTTGTGGCGCGTGGCACGGTGATTCTGGCCGAGCACACCAACTTCACCGGAAACTTTGCTGAAATAGCTTTGCAATGCCTGCAAAGACTCCCAGCAACCAACACAAAATTCACTTACAACACCGATGGTCATACCTTCAACTACCTCGCCCATGATGGATTCAGTACTTGCCTCGTTTTATCTTTTTCTTTTACATAATCTAATTGTGGAATAGCATATCAATGCAATGCGATATATGAGGCACGGACACTAGTGTTTAGATCTTGATAATAATTTAAGAAAATATAATTGATCAAATGCATTTACATGTACTGGTTTCATGTCGGACATTGAACACCTCTAAGGAAATTTATGATTTGATTTATATATTGCTTTCTTTTTCTTTCTAAAATTCTACTATATTATTAGACTTACATGATCTATTATCTTTAGCTTACTGCCTTGTTGCTGTGGAGTCTTTTGATCGCCATATTGCTATGGCCTTTCTTGATCACATCAAGGAGGATTTTACCAAAAGATATGGTGGAGGAAAAGCTGCAACAGCAACATCTAAAAGCTTGAATAAAGAATTTGGGTACTCAAGTTTATAACAATTTTGTGTATAGAAAATGTGTTACAAAAGTTGAATTGCATTAATTAACATGAAGTTTTTTTTATTGTATATGCATGGTAATTTAGACCGAAATTGAAGGAGCATATGCAATATTGTGTGGAACATCCAGAAGAGGTTAGCAAACTTGCGAAAGTAAAAGCTCAGGTTTCTCAAGTTCAAGATGTTATGTTGGAAAATATTGATCAGGTATATATATGTCTAAATTGGATCCTCTGGAGCCGACTTTGAAGCACAGATCGTTAGATTAAGAGAGAAAATTTTATTTGTTTTGTCTGCAGGTCCTTAATCGTCAAGTGAAGATTGATGTTTTGATGGACAAAACTGTCAACCTTCGTGATCAGGTCCTTTTATTTGTTCATCCATAAACCATGCGTCATAGCTAGTGATGAAATTTTCACAAAATCACAAGACCATCGCAGAAAACATAGTACTGTGTTTATTTATGACTGTTCTAGTTCATGAATTAATTACTATATATTTATATTTTTACACACACATGAATAGGGGTCTAGTATAATCTAGTAACCATAAATAACTAAATTACATTGTCAAATTATCTTAACAGATATATACACATAATTTTAAATAAATTAGACATTTATAGTTATGTAACTGATATGGTTTGAATGTGGCTTTGCAGGCACAAGATTTAAGGGGGAATGCAGGGCAATTAAGGAGAAAGATGTGGTTTCAGAACATGAAGATAAAACTAATAGTTCTTGCTATCATAATTGTCATCATTCTAATAATTGTTCTTGTTCTTACATAA

Protein sequence

>MS.gene77223.t1

MAQQSLIYSFVARGTVILAEHTNFTGNFAEIALQCLQRLPATNTKFTYNTDGHTFNYLAHDGFTYCLVAVESFDRHIAMAFLDHIKEDFTKRYGGGKAATATSKSLNKEFGPKLKEHMQYCVEHPEEVSKLAKVKAQVSQVQDVMLENIDQVLNRQVKIDVLMDKTVNLRDQAQDLRGNAGQLRRKMWFQNMKIKLIVLAIIIVIILIIVLVLT