AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81680.t1 MTR_5g084570 94.000 50 3 0 1 50 1 50 1.82e-27 101
MS.gene81680.t1 MTR_5g084540 100.000 26 0 0 148 173 135 160 1.37e-14 67.8
MS.gene81680.t1 MTR_5g084530 74.000 50 12 1 1 50 1 49 6.63e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 83 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTACCTTTGATGCCAAAA+AGG 0.253882 5.1:-66576401 MS.gene81680:CDS
GTTGTAACCTCCACCATATT+TGG 0.288132 5.1:+66576224 None:intergenic
GAAGTAAACGATGCCAAATA+TGG 0.342429 5.1:-66576237 MS.gene81680:CDS
ACCTGCCATGGCAATGGTAT+TGG 0.432416 5.1:+66575926 None:intergenic
GAACCATAGCCAAGAGGCCT+AGG 0.443599 5.1:+66576464 None:intergenic
CATCCTAGGCCTCTTGGCTA+TGG 0.447886 5.1:-66576467 MS.gene81680:CDS
AATCCTCATCCTAGGCCTCT+TGG 0.458683 5.1:-66576473 MS.gene81680:CDS
GCCAATACCATTGCCATGGC+AGG 0.481585 5.1:-66575927 MS.gene81680:CDS
AGTGTTGTCCTTAGCTTGCA+TGG 0.498460 5.1:+66575879 None:intergenic
GGTGGTTACAACCATGGTGG+AGG 0.499176 5.1:-66576033 MS.gene81680:CDS
GGTGGCTACAACCATGGTGG+AGG 0.500815 5.1:-66575997 MS.gene81680:CDS
GGATACAACCACGGTGGTGG+TGG 0.504414 5.1:-66576135 MS.gene81680:CDS
GGTGGATACAACCACGGTGG+TGG 0.511490 5.1:-66576138 MS.gene81680:CDS
AGAAAAGCAATCCTCATCCT+AGG 0.516305 5.1:-66576481 MS.gene81680:CDS
GGATACAACAATGGTGGAGG+TGG 0.518813 5.1:-66576072 MS.gene81680:CDS
GGATACAACAATGGTGGAGG+TGG 0.518813 5.1:-66576114 MS.gene81680:CDS
GGAGGTTACAACCACGGCGG+TGG 0.523190 5.1:-66576213 MS.gene81680:CDS
GGCTACAACCATGGTGGAGG+CGG 0.523424 5.1:-66575994 MS.gene81680:CDS
GGCGGTTACAACCACGGTGG+AGG 0.523588 5.1:-66575955 MS.gene81680:CDS
GGCGGTTACAACCACGGTGG+AGG 0.523588 5.1:-66575976 MS.gene81680:CDS
GGATACAACCACGGTGGTGG+TGG 0.524452 5.1:-66576093 MS.gene81680:CDS
GGTTACAACCACGGTGGTGG+TGG 0.527779 5.1:-66576174 MS.gene81680:CDS
TAGCCAAGAGGCCTAGGATG+AGG 0.538496 5.1:+66576470 None:intergenic
ATTTGTTGCCATGCAAGCTA+AGG 0.539995 5.1:-66575887 MS.gene81680:CDS
AATGGTGGTGGCTACAACCA+TGG 0.544536 5.1:-66576003 MS.gene81680:CDS
GGTTACAACCACGGCGGTGG+TGG 0.544864 5.1:-66576210 MS.gene81680:CDS
GGAGGTGGTTACAATGGTGG+TGG 0.545650 5.1:-66576015 MS.gene81680:CDS
GGTTACAACCATGGTGGAGG+TGG 0.547395 5.1:-66576030 MS.gene81680:CDS
GGAGGTTACAACCACGGTGG+TGG 0.547932 5.1:-66576177 MS.gene81680:CDS
GGATACAACCACGGTGGTGG+TGG 0.553440 5.1:-66576051 MS.gene81680:CDS
TTCCTCAGAAGTCTCAGCTA+GGG 0.554700 5.1:-66576434 MS.gene81680:CDS
GGTGGATACAACCACGGTGG+TGG 0.558361 5.1:-66576054 MS.gene81680:CDS
GGTGGATACAACCACGGTGG+TGG 0.558361 5.1:-66576096 MS.gene81680:CDS
CATGTGAGCAGCACCTGCCA+TGG 0.559419 5.1:+66575914 None:intergenic
TTTCCTCAGAAGTCTCAGCT+AGG 0.560253 5.1:-66576435 MS.gene81680:CDS
CACGGCGGTGGTGGTTACAA+TGG 0.571191 5.1:-66576201 MS.gene81680:CDS
GGTTACAACCACGGTGGAGG+CGG 0.572554 5.1:-66575952 MS.gene81680:CDS
AACGATGCCAAATATGGTGG+AGG 0.573315 5.1:-66576231 MS.gene81680:CDS
GGTTACAACCACGGTGGAGG+CGG 0.574574 5.1:-66575973 MS.gene81680:CDS
GGTGGATACAACAATGGCGG+TGG 0.583166 5.1:-66576156 MS.gene81680:CDS
GGCGGTGGTGGTTACAATGG+TGG 0.594919 5.1:-66576198 MS.gene81680:CDS
GGTTGTAACCGCCTCCACCA+TGG 0.597627 5.1:+66575986 None:intergenic
CATGGTGGAGGTGGTTACAA+TGG 0.597788 5.1:-66576021 MS.gene81680:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.1:-66576075 MS.gene81680:CDS
GGTGGATACAACAATGGTGG+AGG 0.598383 5.1:-66576117 MS.gene81680:CDS
GGTGGTGGTTACAATGGTGG+AGG 0.609526 5.1:-66576195 MS.gene81680:CDS
GGTGGAGGTGGTTACAATGG+TGG 0.613370 5.1:-66576018 MS.gene81680:CDS
AGCAGCACCTGCCATGGCAA+TGG 0.620983 5.1:+66575920 None:intergenic
GGTGGTGGTGGTTACAACCA+TGG 0.640243 5.1:-66576039 MS.gene81680:CDS
GGTGGAGGCGGTTACAACCA+CGG 0.641005 5.1:-66575982 MS.gene81680:CDS
GGTTGCCAATACCATTGCCA+TGG 0.642022 5.1:-66575931 MS.gene81680:CDS
GTCCCTAGCTGAGACTTCTG+AGG 0.645915 5.1:+66576432 None:intergenic
CATTGTAACCACCACCGCCG+TGG 0.646466 5.1:+66576202 None:intergenic
GTAAACGATGCCAAATATGG+TGG 0.647144 5.1:-66576234 MS.gene81680:CDS
CATTGTAACCACCTCCACCA+TGG 0.649002 5.1:+66576022 None:intergenic
TAAGAAGAACCATAGCCAAG+AGG 0.664133 5.1:+66576458 None:intergenic
GGTGGTGGTGGATACAACAA+TGG 0.666044 5.1:-66576162 MS.gene81680:CDS
GGTGGAGGCGGTTACAACCA+CGG 0.674017 5.1:-66575961 MS.gene81680:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.1:-66576078 MS.gene81680:CDS
GGTGGTGGATACAACAATGG+TGG 0.676280 5.1:-66576120 MS.gene81680:CDS
ATTGGCAACCGCCTCCACCG+TGG 0.678280 5.1:+66575944 None:intergenic
GGTTGTAACCGCCTCCACCG+TGG 0.681332 5.1:+66575965 None:intergenic
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.1:-66576081 MS.gene81680:CDS
GGTGGTGGTGGATACAACAA+TGG 0.684345 5.1:-66576123 MS.gene81680:CDS
TATGGTGGAGGTTACAACCA+CGG 0.702708 5.1:-66576219 MS.gene81680:CDS
AATGGTGGAGGTTACAACCA+CGG 0.710899 5.1:-66576183 MS.gene81680:CDS
TGTTGTATCCACCACCACCG+TGG 0.714982 5.1:+66576085 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.1:+66576127 None:intergenic
TGTTGTATCCACCACCACCG+TGG 0.714982 5.1:+66576166 None:intergenic
GGTGGTGGCTACAACCATGG+TGG 0.723784 5.1:-66576000 MS.gene81680:CDS
GGTGGAGGTGGATACAACCA+CGG 0.728168 5.1:-66576060 MS.gene81680:CDS
GGTGGAGGTGGATACAACCA+CGG 0.728168 5.1:-66576102 MS.gene81680:CDS
GGTGGTGGTTACAACCATGG+TGG 0.730633 5.1:-66576036 MS.gene81680:CDS
AATGGCGGTGGATACAACCA+CGG 0.732761 5.1:-66576144 MS.gene81680:CDS
GGTGGTGGATACAACAATGG+CGG 0.734675 5.1:-66576159 MS.gene81680:CDS
GGTTGTAACCACCACCACCG+TGG 0.740537 5.1:+66576043 None:intergenic
GGCGGTGGATACAACCACGG+TGG 0.764343 5.1:-66576141 MS.gene81680:CDS
GGAGGCGGTTACAACCACGG+TGG 0.788813 5.1:-66575979 MS.gene81680:CDS
GGAGGCGGTTACAACCACGG+TGG 0.797498 5.1:-66575958 MS.gene81680:CDS
GGTGGAGGTTACAACCACGG+TGG 0.812517 5.1:-66576180 MS.gene81680:CDS
GGAGGTGGATACAACCACGG+TGG 0.823069 5.1:-66576057 MS.gene81680:CDS
GGAGGTGGATACAACCACGG+TGG 0.823069 5.1:-66576099 MS.gene81680:CDS
GGTGGAGGTTACAACCACGG+CGG 0.825418 5.1:-66576216 MS.gene81680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATATAGCTCACCCTTTT+TGG + chr5.1:66575976-66575995 None:intergenic 30.0%
AACTACCTTTGATGCCAAAA+AGG - chr5.1:66575959-66575978 MS.gene81680:CDS 35.0%
ACTACCTTTGATGCCAAAAA+GGG - chr5.1:66575960-66575979 MS.gene81680:CDS 35.0%
GAAGTAAACGATGCCAAATA+TGG - chr5.1:66576123-66576142 MS.gene81680:CDS 35.0%
AGAAAAGCAATCCTCATCCT+AGG - chr5.1:66575879-66575898 MS.gene81680:CDS 40.0%
GTAAACGATGCCAAATATGG+TGG - chr5.1:66576126-66576145 MS.gene81680:CDS 40.0%
GTTGTAACCTCCACCATATT+TGG + chr5.1:66576139-66576158 None:intergenic 40.0%
TAAGAAGAACCATAGCCAAG+AGG + chr5.1:66575905-66575924 None:intergenic 40.0%
! ATTTGTTGCCATGCAAGCTA+AGG - chr5.1:66576473-66576492 MS.gene81680:CDS 40.0%
AACGATGCCAAATATGGTGG+AGG - chr5.1:66576129-66576148 MS.gene81680:CDS 45.0%
TTCCTCAGAAGTCTCAGCTA+GGG - chr5.1:66575926-66575945 MS.gene81680:CDS 45.0%
TTTCCTCAGAAGTCTCAGCT+AGG - chr5.1:66575925-66575944 MS.gene81680:CDS 45.0%
! AATGGTGGAGGTTACAACCA+CGG - chr5.1:66576177-66576196 MS.gene81680:CDS 45.0%
! TATGGTGGAGGTTACAACCA+CGG - chr5.1:66576141-66576160 MS.gene81680:CDS 45.0%
!! AGTGTTGTCCTTAGCTTGCA+TGG + chr5.1:66576484-66576503 None:intergenic 45.0%
!! CTCACCCTTTTTGGCATCAA+AGG + chr5.1:66575967-66575986 None:intergenic 45.0%
AATCCTCATCCTAGGCCTCT+TGG - chr5.1:66575887-66575906 MS.gene81680:CDS 50.0%
AATGGCGGTGGATACAACCA+CGG - chr5.1:66576216-66576235 MS.gene81680:CDS 50.0%
ACCTGCCATGGCAATGGTAT+TGG + chr5.1:66576437-66576456 None:intergenic 50.0%
CATTGTAACCACCTCCACCA+TGG + chr5.1:66576341-66576360 None:intergenic 50.0%
GGTGGATACAACAATGGTGG+AGG - chr5.1:66576243-66576262 MS.gene81680:CDS 50.0%
GGTTGCCAATACCATTGCCA+TGG - chr5.1:66576429-66576448 MS.gene81680:CDS 50.0%
! CATGGTGGAGGTGGTTACAA+TGG - chr5.1:66576339-66576358 MS.gene81680:intron 50.0%
! GGATACAACAATGGTGGAGG+TGG - chr5.1:66576246-66576265 MS.gene81680:CDS 50.0%
!! AATGGTGGTGGCTACAACCA+TGG - chr5.1:66576357-66576376 MS.gene81680:intron 50.0%
!! GGTGGTGGATACAACAATGG+CGG - chr5.1:66576201-66576220 MS.gene81680:CDS 50.0%
!! GGTGGTGGATACAACAATGG+TGG - chr5.1:66576201-66576220 MS.gene81680:CDS 50.0%
!! GGTGGTGGTGGATACAACAA+TGG - chr5.1:66576198-66576217 MS.gene81680:CDS 50.0%
CATCCTAGGCCTCTTGGCTA+TGG - chr5.1:66575893-66575912 MS.gene81680:CDS 55.0%
GAACCATAGCCAAGAGGCCT+AGG + chr5.1:66575899-66575918 None:intergenic 55.0%
GCCAATACCATTGCCATGGC+AGG - chr5.1:66576433-66576452 MS.gene81680:CDS 55.0%
GGAGGTGGTTACAATGGTGG+TGG - chr5.1:66576345-66576364 MS.gene81680:intron 55.0%
GGTGGAGGTGGATACAACCA+CGG - chr5.1:66576258-66576277 MS.gene81680:CDS 55.0%
GGTGGAGGTGGTTACAATGG+TGG - chr5.1:66576342-66576361 MS.gene81680:intron 55.0%
GGTGGATACAACAATGGCGG+TGG - chr5.1:66576204-66576223 MS.gene81680:CDS 55.0%
GGTGGTTACAACCATGGTGG+AGG - chr5.1:66576327-66576346 MS.gene81680:intron 55.0%
GTCCCTAGCTGAGACTTCTG+AGG + chr5.1:66575931-66575950 None:intergenic 55.0%
TAGCCAAGAGGCCTAGGATG+AGG + chr5.1:66575893-66575912 None:intergenic 55.0%
TGTTGTATCCACCACCACCG+TGG + chr5.1:66576197-66576216 None:intergenic 55.0%
! GGTGGTGGTTACAACCATGG+TGG - chr5.1:66576324-66576343 MS.gene81680:intron 55.0%
! GGTGGTGGTTACAATGGTGG+AGG - chr5.1:66576165-66576184 MS.gene81680:CDS 55.0%
! GGTTACAACCATGGTGGAGG+TGG - chr5.1:66576330-66576349 MS.gene81680:intron 55.0%
!! GGTGGTGGTGGTTACAACCA+TGG - chr5.1:66576321-66576340 MS.gene81680:intron 55.0%
AGCAGCACCTGCCATGGCAA+TGG + chr5.1:66576443-66576462 None:intergenic 60.0%
CATGTGAGCAGCACCTGCCA+TGG + chr5.1:66576449-66576468 None:intergenic 60.0%
CATTGTAACCACCACCGCCG+TGG + chr5.1:66576161-66576180 None:intergenic 60.0%
GGAGGTGGATACAACCACGG+TGG - chr5.1:66576261-66576280 MS.gene81680:intron 60.0%
GGAGGTTACAACCACGGTGG+TGG - chr5.1:66576183-66576202 MS.gene81680:CDS 60.0%
GGTGGAGGCGGTTACAACCA+CGG - chr5.1:66576378-66576397 MS.gene81680:intron 60.0%
GGTGGAGGTTACAACCACGG+CGG - chr5.1:66576144-66576163 MS.gene81680:CDS 60.0%
GGTGGAGGTTACAACCACGG+TGG - chr5.1:66576144-66576163 MS.gene81680:CDS 60.0%
GGTGGATACAACCACGGTGG+TGG - chr5.1:66576222-66576241 MS.gene81680:CDS 60.0%
GGTGGCTACAACCATGGTGG+AGG - chr5.1:66576363-66576382 MS.gene81680:intron 60.0%
GGTTACAACCACGGTGGAGG+CGG - chr5.1:66576387-66576406 MS.gene81680:intron 60.0%
GGTTGTAACCACCACCACCG+TGG + chr5.1:66576320-66576339 None:intergenic 60.0%
GGTTGTAACCGCCTCCACCA+TGG + chr5.1:66576377-66576396 None:intergenic 60.0%
! CACGGCGGTGGTGGTTACAA+TGG - chr5.1:66576159-66576178 MS.gene81680:CDS 60.0%
! GGATACAACCACGGTGGTGG+TGG - chr5.1:66576225-66576244 MS.gene81680:CDS 60.0%
! GGCGGTGGTGGTTACAATGG+TGG - chr5.1:66576162-66576181 MS.gene81680:CDS 60.0%
! GGCTACAACCATGGTGGAGG+CGG - chr5.1:66576366-66576385 MS.gene81680:intron 60.0%
! GGTGGTGGCTACAACCATGG+TGG - chr5.1:66576360-66576379 MS.gene81680:intron 60.0%
! GGTTACAACCACGGTGGTGG+TGG - chr5.1:66576186-66576205 MS.gene81680:CDS 60.0%
ATTGGCAACCGCCTCCACCG+TGG + chr5.1:66576419-66576438 None:intergenic 65.0%
GGAGGCGGTTACAACCACGG+TGG - chr5.1:66576381-66576400 MS.gene81680:intron 65.0%
GGAGGTTACAACCACGGCGG+TGG - chr5.1:66576147-66576166 MS.gene81680:CDS 65.0%
GGCGGTGGATACAACCACGG+TGG - chr5.1:66576219-66576238 MS.gene81680:CDS 65.0%
GGCGGTTACAACCACGGTGG+AGG - chr5.1:66576384-66576403 MS.gene81680:intron 65.0%
GGTTACAACCACGGCGGTGG+TGG - chr5.1:66576150-66576169 MS.gene81680:CDS 65.0%
GGTTGTAACCGCCTCCACCG+TGG + chr5.1:66576398-66576417 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 66575870 66576512 66575870 ID=MS.gene81680
chr5.1 mRNA 66575870 66576512 66575870 ID=MS.gene81680.t1;Parent=MS.gene81680
chr5.1 exon 66576401 66576512 66576401 ID=MS.gene81680.t1.exon1;Parent=MS.gene81680.t1
chr5.1 CDS 66576401 66576512 66576401 ID=cds.MS.gene81680.t1;Parent=MS.gene81680.t1
chr5.1 exon 66575870 66576279 66575870 ID=MS.gene81680.t1.exon2;Parent=MS.gene81680.t1
chr5.1 CDS 66575870 66576279 66575870 ID=cds.MS.gene81680.t1;Parent=MS.gene81680.t1
Gene Sequence

>MS.gene81680

ATGGATTCGAGAAAAGCAATCCTCATCCTAGGCCTCTTGGCTATGGTTCTTCTTATTTCCTCAGAAGTCTCAGCTAGGGACTTAACTGAAACTACCTTTGATGCCAAAAAGGGTGAGCTATATATTATTTTTCTTTATAAATACTTTCACGAGCATTTTCTATATTGTTTAAATTCTTTTCTAGTTAATTATATATAAAGTAATGATTCATATTATATGTGCTGTGTTTGCAGAAGTTGTTGAAAAGACTAATGAAGTAAACGATGCCAAATATGGTGGAGGTTACAACCACGGCGGTGGTGGTTACAATGGTGGAGGTTACAACCACGGTGGTGGTGGATACAACAATGGCGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGATACAACCACGGTGGTGGTGGATACAACAATGGTGGAGGTGGATACAACCACGGTGGTGGTGGTTACAACCATGGTGGAGGTGGTTACAATGGTGGTGGCTACAACCATGGTGGAGGCGGTTACAACCACGGTGGAGGCGGTTACAACCACGGTGGAGGCGGTTGCCAATACCATTGCCATGGCAGGTGCTGCTCACATGCTGAATTTGTTGCCATGCAAGCTAAGGACAACACTCAGAATTGA

Protein sequence

>MS.gene81680.t1

MDSRKAILILGLLAMVLLISSEVSARDLTETTFDAKKEVVEKTNEVNDAKYGGGYNHGGGGYNGGGYNHGGGGYNNGGGYNHGGGGYNNGGGGYNHGGGGYNNGGGGYNHGGGGYNHGGGGYNGGGYNHGGGGYNHGGGGYNHGGGGCQYHCHGRCCSHAEFVAMQAKDNTQN