Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92348.t1 | XP_003610640.1 | 95.9 | 73 | 1 | 1 | 1 | 73 | 1 | 71 | 1.70E-25 | 124.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92348.t1 | G7K0Z0 | 95.9 | 73 | 1 | 1 | 1 | 73 | 1 | 71 | 1.2e-25 | 124.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene92348 | 0.803132 | 4.03E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene92348 | 0.8176 | 3.03E-52 | -1.69E-46 |
| MS.gene049254 | MS.gene92348 | 0.82318 | 1.59E-53 | -1.69E-46 |
| MS.gene049283 | MS.gene92348 | 0.814174 | 1.76E-51 | -1.69E-46 |
| MS.gene049499 | MS.gene92348 | 0.821424 | 4.07E-53 | -1.69E-46 |
| MS.gene049652 | MS.gene92348 | 0.814245 | 1.70E-51 | -1.69E-46 |
| MS.gene049680 | MS.gene92348 | 0.80874 | 2.67E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene92348 | 0.827697 | 1.36E-54 | -1.69E-46 |
| MS.gene049807 | MS.gene92348 | 0.811264 | 7.64E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene92348 | 0.829236 | 5.76E-55 | -1.69E-46 |
| MS.gene050561 | MS.gene92348 | 0.815779 | 7.76E-52 | -1.69E-46 |
| MS.gene050562 | MS.gene92348 | 0.821058 | 4.94E-53 | -1.69E-46 |
| MS.gene050945 | MS.gene92348 | 0.801059 | 1.08E-48 | -1.69E-46 |
| MS.gene051019 | MS.gene92348 | 0.806847 | 6.74E-50 | -1.69E-46 |
| MS.gene051332 | MS.gene92348 | 0.819473 | 1.14E-52 | -1.69E-46 |
| MS.gene051699 | MS.gene92348 | 0.827251 | 1.73E-54 | -1.69E-46 |
| MS.gene051744 | MS.gene92348 | 0.81783 | 2.69E-52 | -1.69E-46 |
| MS.gene051832 | MS.gene92348 | 0.854251 | 1.39E-61 | -1.69E-46 |
| MS.gene051975 | MS.gene92348 | 0.805986 | 1.02E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene92348 | 0.845164 | 4.82E-59 | -1.69E-46 |
| MS.gene053094 | MS.gene92348 | 0.839141 | 1.89E-57 | -1.69E-46 |
| MS.gene053963 | MS.gene92348 | 0.815787 | 7.73E-52 | -1.69E-46 |
| MS.gene054222 | MS.gene92348 | 0.843074 | 1.75E-58 | -1.69E-46 |
| MS.gene054490 | MS.gene92348 | 0.802819 | 4.68E-49 | -1.69E-46 |
| MS.gene054576 | MS.gene92348 | 0.811086 | 8.35E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene92348 | 0.818698 | 1.71E-52 | -1.69E-46 |
| MS.gene054893 | MS.gene92348 | 0.820825 | 5.59E-53 | -1.69E-46 |
| MS.gene054957 | MS.gene92348 | 0.80131 | 9.56E-49 | -1.69E-46 |
| MS.gene055150 | MS.gene92348 | 0.803296 | 3.73E-49 | -1.69E-46 |
| MS.gene055724 | MS.gene92348 | 0.800302 | 1.54E-48 | -1.69E-46 |
| MS.gene056082 | MS.gene92348 | 0.815434 | 9.26E-52 | -1.69E-46 |
| MS.gene056677 | MS.gene92348 | 0.824617 | 7.33E-54 | -1.69E-46 |
| MS.gene056699 | MS.gene92348 | 0.835818 | 1.35E-56 | -1.69E-46 |
| MS.gene05686 | MS.gene92348 | 0.824918 | 6.22E-54 | -1.69E-46 |
| MS.gene056986 | MS.gene92348 | 0.807362 | 5.25E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene92348 | 0.877604 | 5.50E-69 | -1.69E-46 |
| MS.gene057208 | MS.gene92348 | 0.812951 | 3.27E-51 | -1.69E-46 |
| MS.gene058279 | MS.gene92348 | 0.822499 | 2.29E-53 | -1.69E-46 |
| MS.gene058592 | MS.gene92348 | 0.818899 | 1.54E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene92348 | 0.830371 | 3.05E-55 | -1.69E-46 |
| MS.gene059774 | MS.gene92348 | 0.819337 | 1.22E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene92348 | -0.896527 | 3.39E-76 | -1.69E-46 |
| MS.gene059901 | MS.gene92348 | -0.804174 | 2.45E-49 | -1.69E-46 |
| MS.gene059965 | MS.gene92348 | 0.803507 | 3.37E-49 | -1.69E-46 |
| MS.gene060012 | MS.gene92348 | 0.801391 | 9.20E-49 | -1.69E-46 |
| MS.gene060338 | MS.gene92348 | 0.819297 | 1.25E-52 | -1.69E-46 |
| MS.gene060365 | MS.gene92348 | 0.812613 | 3.88E-51 | -1.69E-46 |
| MS.gene060412 | MS.gene92348 | 0.814976 | 1.17E-51 | -1.69E-46 |
| MS.gene060697 | MS.gene92348 | 0.813047 | 3.12E-51 | -1.69E-46 |
| MS.gene060716 | MS.gene92348 | 0.800332 | 1.51E-48 | -1.69E-46 |
| MS.gene060935 | MS.gene92348 | 0.804803 | 1.81E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene92348 | 0.821612 | 3.68E-53 | -1.69E-46 |
| MS.gene061100 | MS.gene92348 | 0.805486 | 1.30E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene92348 | 0.88047 | 5.35E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92348.t1 | MTR_5g005330 | 95.890 | 73 | 1 | 1 | 1 | 73 | 1 | 71 | 1.45e-42 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92348.t1 | AT3G19660 | 58.333 | 60 | 22 | 1 | 1 | 60 | 1 | 57 | 4.49e-14 | 60.5 |
Find 15 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGTCCATCTTTATGTA+TGG | 0.307750 | 5.1:+381791 | None:intergenic |
| AATAAGAATAAGAAATTATA+TGG | 0.312903 | 5.1:+381658 | None:intergenic |
| ATTATTATCAGCAGCTTTCA+AGG | 0.340671 | 5.1:+381712 | None:intergenic |
| GGCAAATCAGCTGGGAAATC+TGG | 0.373442 | 5.1:-381870 | MS.gene92348:CDS |
| GACGTTGTGCTTGGTCTTAG+TGG | 0.439128 | 5.1:+381682 | None:intergenic |
| TAGAAGAATGGCAAATCAGC+TGG | 0.458624 | 5.1:-381879 | None:intergenic |
| AAAGAAGCCATACATAAAGA+TGG | 0.499886 | 5.1:-381798 | MS.gene92348:CDS |
| ACGTTGTGCTTGGTCTTAGT+GGG | 0.536017 | 5.1:+381683 | None:intergenic |
| TCAACGTTGAAGATGAAGAA+AGG | 0.541828 | 5.1:-381821 | MS.gene92348:CDS |
| TTATATGGTGACGTTGTGCT+TGG | 0.549241 | 5.1:+381673 | None:intergenic |
| GGTGGAGTCAATAAAGTCAA+AGG | 0.580028 | 5.1:-381849 | MS.gene92348:CDS |
| AGAAGAATGGCAAATCAGCT+GGG | 0.603317 | 5.1:-381878 | None:intergenic |
| TTGTGCTTGGTCTTAGTGGG+TGG | 0.613312 | 5.1:+381686 | None:intergenic |
| AAATCAGCTGGGAAATCTGG+TGG | 0.634337 | 5.1:-381867 | MS.gene92348:CDS |
| GGACAAGAGTGCAAGTGTGA+AGG | 0.667874 | 5.1:-381777 | MS.gene92348:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAAGCCATACATAAAGA+TGG | - | chr5.1:381747-381766 | MS.gene92348:CDS | 30.0% | |
| ATTATTATCAGCAGCTTTCA+AGG | + | chr5.1:381836-381855 | None:intergenic | 30.0% | |
| CTCTTGTCCATCTTTATGTA+TGG | + | chr5.1:381757-381776 | None:intergenic | 35.0% | |
| TCAACGTTGAAGATGAAGAA+AGG | - | chr5.1:381724-381743 | MS.gene92348:CDS | 35.0% | |
| GGTGGAGTCAATAAAGTCAA+AGG | - | chr5.1:381696-381715 | MS.gene92348:CDS | 40.0% | |
| ! | TTATATGGTGACGTTGTGCT+TGG | + | chr5.1:381875-381894 | None:intergenic | 40.0% |
| AAATCAGCTGGGAAATCTGG+TGG | - | chr5.1:381678-381697 | MS.gene92348:CDS | 45.0% | |
| !! | ACGTTGTGCTTGGTCTTAGT+GGG | + | chr5.1:381865-381884 | None:intergenic | 45.0% |
| GGCAAATCAGCTGGGAAATC+TGG | - | chr5.1:381675-381694 | MS.gene92348:CDS | 50.0% | |
| ! | GGACAAGAGTGCAAGTGTGA+AGG | - | chr5.1:381768-381787 | MS.gene92348:CDS | 50.0% |
| !! | GACGTTGTGCTTGGTCTTAG+TGG | + | chr5.1:381866-381885 | None:intergenic | 50.0% |
| !! | TTGTGCTTGGTCTTAGTGGG+TGG | + | chr5.1:381862-381881 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 381673 | 381894 | 381673 | ID=MS.gene92348 |
| chr5.1 | mRNA | 381673 | 381894 | 381673 | ID=MS.gene92348.t1;Parent=MS.gene92348 |
| chr5.1 | exon | 381673 | 381894 | 381673 | ID=MS.gene92348.t1.exon1;Parent=MS.gene92348.t1 |
| chr5.1 | CDS | 381673 | 381894 | 381673 | ID=cds.MS.gene92348.t1;Parent=MS.gene92348.t1 |
| Gene Sequence |
| Protein sequence |
