Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93743.t1 | RHN43932.1 | 96.9 | 127 | 2 | 1 | 1 | 125 | 32 | 158 | 3.70E-57 | 230.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93743.t1 | A0A396GS31 | 96.9 | 127 | 2 | 1 | 1 | 125 | 32 | 158 | 2.7e-57 | 230.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene93743 | 0.804865 | 1.76E-49 | -1.69E-46 |
| MS.gene049378 | MS.gene93743 | 0.812597 | 3.91E-51 | -1.69E-46 |
| MS.gene049577 | MS.gene93743 | 0.8169 | 4.36E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene93743 | 0.800046 | 1.73E-48 | -1.69E-46 |
| MS.gene050330 | MS.gene93743 | -0.829515 | 4.93E-55 | -1.69E-46 |
| MS.gene050404 | MS.gene93743 | 0.854356 | 1.30E-61 | -1.69E-46 |
| MS.gene050406 | MS.gene93743 | 0.859814 | 3.19E-63 | -1.69E-46 |
| MS.gene050426 | MS.gene93743 | -0.818157 | 2.27E-52 | -1.69E-46 |
| MS.gene050464 | MS.gene93743 | 0.808194 | 3.49E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene93743 | 0.824055 | 9.93E-54 | -1.69E-46 |
| MS.gene050642 | MS.gene93743 | 0.858461 | 8.11E-63 | -1.69E-46 |
| MS.gene051171 | MS.gene93743 | 0.852992 | 3.21E-61 | -1.69E-46 |
| MS.gene051240 | MS.gene93743 | 0.841561 | 4.41E-58 | -1.69E-46 |
| MS.gene051242 | MS.gene93743 | 0.842715 | 2.18E-58 | -1.69E-46 |
| MS.gene05137 | MS.gene93743 | 0.812395 | 4.33E-51 | -1.69E-46 |
| MS.gene051582 | MS.gene93743 | 0.825841 | 3.76E-54 | -1.69E-46 |
| MS.gene051983 | MS.gene93743 | 0.840692 | 7.46E-58 | -1.69E-46 |
| MS.gene052011 | MS.gene93743 | -0.811294 | 7.52E-51 | -1.69E-46 |
| MS.gene052077 | MS.gene93743 | 0.84725 | 1.30E-59 | -1.69E-46 |
| MS.gene052078 | MS.gene93743 | 0.80617 | 9.37E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene93743 | 0.84952 | 3.07E-60 | -1.69E-46 |
| MS.gene052253 | MS.gene93743 | 0.801176 | 1.02E-48 | -1.69E-46 |
| MS.gene052309 | MS.gene93743 | -0.851171 | 1.06E-60 | -1.69E-46 |
| MS.gene052340 | MS.gene93743 | 0.820899 | 5.38E-53 | -1.69E-46 |
| MS.gene052389 | MS.gene93743 | 0.81342 | 2.58E-51 | -1.69E-46 |
| MS.gene052590 | MS.gene93743 | 0.812391 | 4.34E-51 | -1.69E-46 |
| MS.gene052600 | MS.gene93743 | 0.818492 | 1.91E-52 | -1.69E-46 |
| MS.gene053330 | MS.gene93743 | 0.865711 | 4.84E-65 | -1.69E-46 |
| MS.gene053557 | MS.gene93743 | 0.80426 | 2.35E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene93743 | 0.840448 | 8.65E-58 | -1.69E-46 |
| MS.gene053907 | MS.gene93743 | 0.854805 | 9.64E-62 | -1.69E-46 |
| MS.gene053971 | MS.gene93743 | 0.838087 | 3.54E-57 | -1.69E-46 |
| MS.gene054930 | MS.gene93743 | 0.817399 | 3.37E-52 | -1.69E-46 |
| MS.gene054983 | MS.gene93743 | 0.827863 | 1.24E-54 | -1.69E-46 |
| MS.gene055428 | MS.gene93743 | 0.821538 | 3.83E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene93743 | 0.85873 | 6.74E-63 | -1.69E-46 |
| MS.gene056087 | MS.gene93743 | 0.80875 | 2.66E-50 | -1.69E-46 |
| MS.gene056305 | MS.gene93743 | 0.811109 | 8.25E-51 | -1.69E-46 |
| MS.gene056451 | MS.gene93743 | 0.822274 | 2.59E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene93743 | 0.832462 | 9.32E-56 | -1.69E-46 |
| MS.gene056633 | MS.gene93743 | 0.860878 | 1.52E-63 | -1.69E-46 |
| MS.gene056664 | MS.gene93743 | 0.831778 | 1.38E-55 | -1.69E-46 |
| MS.gene056899 | MS.gene93743 | -0.802113 | 6.54E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene93743 | 0.838925 | 2.15E-57 | -1.69E-46 |
| MS.gene05702 | MS.gene93743 | 0.827477 | 1.53E-54 | -1.69E-46 |
| MS.gene057121 | MS.gene93743 | 0.805317 | 1.42E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene93743 | 0.812196 | 4.79E-51 | -1.69E-46 |
| MS.gene058142 | MS.gene93743 | 0.874825 | 4.99E-68 | -1.69E-46 |
| MS.gene058201 | MS.gene93743 | 0.801141 | 1.04E-48 | -1.69E-46 |
| MS.gene058233 | MS.gene93743 | 0.850296 | 1.86E-60 | -1.69E-46 |
| MS.gene058276 | MS.gene93743 | 0.868135 | 8.18E-66 | -1.69E-46 |
| MS.gene058710 | MS.gene93743 | 0.807686 | 4.48E-50 | -1.69E-46 |
| MS.gene058746 | MS.gene93743 | 0.873813 | 1.10E-67 | -1.69E-46 |
| MS.gene058749 | MS.gene93743 | 0.822918 | 1.83E-53 | -1.69E-46 |
| MS.gene059011 | MS.gene93743 | 0.801174 | 1.02E-48 | -1.69E-46 |
| MS.gene059247 | MS.gene93743 | 0.846632 | 1.92E-59 | -1.69E-46 |
| MS.gene059285 | MS.gene93743 | 0.812778 | 3.57E-51 | -1.69E-46 |
| MS.gene059511 | MS.gene93743 | 0.803389 | 3.57E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene93743 | 0.822119 | 2.81E-53 | -1.69E-46 |
| MS.gene059894 | MS.gene93743 | 0.852935 | 3.33E-61 | -1.69E-46 |
| MS.gene059899 | MS.gene93743 | 0.831415 | 1.69E-55 | -1.69E-46 |
| MS.gene060407 | MS.gene93743 | 0.845685 | 3.48E-59 | -1.69E-46 |
| MS.gene061049 | MS.gene93743 | 0.863705 | 2.06E-64 | -1.69E-46 |
| MS.gene061114 | MS.gene93743 | -0.829281 | 5.62E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93743.t1 | MTR_8g107430 | 99.065 | 107 | 1 | 0 | 19 | 125 | 90 | 196 | 1.12e-72 | 215 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93743.t1 | AT3G17930 | 78.095 | 105 | 23 | 0 | 21 | 125 | 84 | 188 | 1.10e-57 | 177 |
Find 27 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCAACTTGTGCTGGTTT+TGG | 0.236008 | 4.2:-148686 | MS.gene93743:CDS |
| TTCAAACTCCTCCAATGATT+TGG | 0.257000 | 4.2:-149076 | None:intergenic |
| CTTGTAGCTCTGCTTCTGTT+AGG | 0.288048 | 4.2:+147812 | None:intergenic |
| CTTAGTCTGATTATTATTAG+AGG | 0.327985 | 4.2:+149041 | None:intergenic |
| AGGAAAGATGTCATATTAAT+CGG | 0.384907 | 4.2:-148909 | MS.gene93743:CDS |
| CTTGGCATTGCCTTAAAATC+CGG | 0.397706 | 4.2:-148870 | MS.gene93743:CDS |
| TTACAGACTAAGGCACCATT+TGG | 0.404439 | 4.2:-148939 | MS.gene93743:intron |
| TTGTAGCTCTGCTTCTGTTA+GGG | 0.423721 | 4.2:+147813 | None:intergenic |
| GGACTTGGAGTTACTTTCCT+TGG | 0.438033 | 4.2:-148888 | MS.gene93743:CDS |
| AAATGTTGTTCAACTTGTGC+TGG | 0.438263 | 4.2:-148692 | MS.gene93743:CDS |
| AGGCACCATTTGGATATTCA+AGG | 0.473273 | 4.2:-148929 | MS.gene93743:CDS |
| CTCTAATAATAATCAGACTA+AGG | 0.483910 | 4.2:-149040 | MS.gene93743:CDS |
| CTCCTCCAATGATTTGGATC+AGG | 0.484089 | 4.2:-149070 | None:intergenic |
| GGTGTCCTGATCCAAATCAT+TGG | 0.489290 | 4.2:+149065 | None:intergenic |
| AGCTTGTAGTGGATCAAATC+CGG | 0.498185 | 4.2:+148717 | None:intergenic |
| TCTTTCCTTGAATATCCAAA+TGG | 0.501246 | 4.2:+148924 | None:intergenic |
| GATGTCATATTAATCGGACT+TGG | 0.523993 | 4.2:-148903 | MS.gene93743:CDS |
| GAGTTGAGATACCTCAAGTC+CGG | 0.544969 | 4.2:+148851 | None:intergenic |
| TGCCTTAAAATCCGGACTTG+AGG | 0.557648 | 4.2:-148862 | MS.gene93743:intron |
| ACAACATTTCCAGCTTGTAG+TGG | 0.569187 | 4.2:+148706 | None:intergenic |
| GGATTTGATCCACTACAAGC+TGG | 0.576522 | 4.2:-148715 | MS.gene93743:CDS |
| TGACTACGAAAGTAAAGTCA+TGG | 0.582776 | 4.2:-148587 | MS.gene93743:CDS |
| AGTCTGATTATTATTAGAGG+AGG | 0.586722 | 4.2:+149044 | None:intergenic |
| AGTACTGCTTCAAGAAGTCG+AGG | 0.646449 | 4.2:-147791 | MS.gene93743:CDS |
| TATCTTCAGAGTCTCAAACA+AGG | 0.652304 | 4.2:-148635 | MS.gene93743:CDS |
| TTGAGATCCATCCAACAGTG+AGG | 0.728057 | 4.2:+148662 | None:intergenic |
| CTACGAAAGTAAAGTCATGG+AGG | 0.745272 | 4.2:-148584 | MS.gene93743:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTCATTATTGATTTATTT+GGG | - | chr4.2:148641-148660 | MS.gene93743:CDS | 10.0% |
| !! | CAAATAAATCAATAATGAAA+AGG | + | chr4.2:148642-148661 | None:intergenic | 15.0% |
| !!! | AATGTAAAACATTTTGAATA+AGG | + | chr4.2:148715-148734 | None:intergenic | 15.0% |
| !!! | CTTTTCATTATTGATTTATT+TGG | - | chr4.2:148640-148659 | MS.gene93743:CDS | 15.0% |
| !!! | TATTTACTGTAAACAATTTT+AGG | + | chr4.2:148470-148489 | None:intergenic | 15.0% |
| !! | ACTTATATGCTTAATATTAC+TGG | - | chr4.2:147842-147861 | MS.gene93743:intron | 20.0% |
| !! | ATAGAGGTAAAATTATTCTA+TGG | + | chr4.2:148979-148998 | None:intergenic | 20.0% |
| !! | CTTATATGCTTAATATTACT+GGG | - | chr4.2:147843-147862 | MS.gene93743:intron | 20.0% |
| !!! | TAAAACATTTTGAATAAGGT+TGG | + | chr4.2:148711-148730 | None:intergenic | 20.0% |
| !!! | TGTTTACAGTAAATATGTTT+TGG | - | chr4.2:148475-148494 | MS.gene93743:intron | 20.0% |
| ! | AATGAAATATTTCTTTCCGA+AGG | - | chr4.2:148882-148901 | MS.gene93743:CDS | 25.0% |
| ! | AGGAAAGATGTCATATTAAT+CGG | - | chr4.2:147927-147946 | MS.gene93743:intron | 25.0% |
| ! | ATGACTGAAGTTATAAACTT+GGG | - | chr4.2:148910-148929 | MS.gene93743:CDS | 25.0% |
| ! | CTCTAATAATAATCAGACTA+AGG | - | chr4.2:147796-147815 | MS.gene93743:CDS | 25.0% |
| ! | CTTAGTCTGATTATTATTAG+AGG | + | chr4.2:147798-147817 | None:intergenic | 25.0% |
| ! | CTTATTAACTCAGATATCAA+AGG | + | chr4.2:148740-148759 | None:intergenic | 25.0% |
| ! | GAATAATATCTTCTGACTTA+CGG | + | chr4.2:148516-148535 | None:intergenic | 25.0% |
| ! | GAGGTAAAATAATACTAACA+TGG | - | chr4.2:148271-148290 | MS.gene93743:intron | 25.0% |
| !! | AGCATGATATTTTCCTTTTT+GGG | + | chr4.2:148412-148431 | None:intergenic | 25.0% |
| !! | ATATATATTTTGCAGTTTGC+CGG | - | chr4.2:148100-148119 | MS.gene93743:intron | 25.0% |
| !! | TAGCATGATATTTTCCTTTT+TGG | + | chr4.2:148413-148432 | None:intergenic | 25.0% |
| AATATGTATGTAGTAGTCTG+CGG | - | chr4.2:148361-148380 | MS.gene93743:intron | 30.0% | |
| AGTCTGATTATTATTAGAGG+AGG | + | chr4.2:147795-147814 | None:intergenic | 30.0% | |
| ATCATGCAACTATTCTTTCT+TGG | - | chr4.2:148075-148094 | MS.gene93743:intron | 30.0% | |
| ATGTACCATGCGAAATAAAA+AGG | + | chr4.2:148554-148573 | None:intergenic | 30.0% | |
| GATGACTGAAGTTATAAACT+TGG | - | chr4.2:148909-148928 | MS.gene93743:CDS | 30.0% | |
| GCATATAAGTAGAGTATATG+AGG | + | chr4.2:147832-147851 | None:intergenic | 30.0% | |
| GGCATGCTTAAAACAATAAA+AGG | + | chr4.2:148300-148319 | None:intergenic | 30.0% | |
| TCTTTCCTTGAATATCCAAA+TGG | + | chr4.2:147915-147934 | None:intergenic | 30.0% | |
| ! | CTAGTATTTGTTACAGACTA+AGG | - | chr4.2:147887-147906 | MS.gene93743:intron | 30.0% |
| !! | TTGTACCTTTTTATTTCGCA+TGG | - | chr4.2:148546-148565 | MS.gene93743:intron | 30.0% |
| !!! | TTATTGTTTTAAGCATGCCT+GGG | - | chr4.2:148301-148320 | MS.gene93743:intron | 30.0% |
| !!! | TTTATTGTTTTAAGCATGCC+TGG | - | chr4.2:148300-148319 | MS.gene93743:intron | 30.0% |
| AAATGTTGTTCAACTTGTGC+TGG | - | chr4.2:148144-148163 | MS.gene93743:intron | 35.0% | |
| ACTTCAGTCATCTATACCTT+CGG | + | chr4.2:148901-148920 | None:intergenic | 35.0% | |
| GAATGTTTAACAGTCTCTCA+GGG | + | chr4.2:148832-148851 | None:intergenic | 35.0% | |
| GATGTCATATTAATCGGACT+TGG | - | chr4.2:147933-147952 | MS.gene93743:intron | 35.0% | |
| GTCTTCAAAATGCTGAAATG+TGG | - | chr4.2:148383-148402 | MS.gene93743:intron | 35.0% | |
| TATCTTCAGAGTCTCAAACA+AGG | - | chr4.2:148201-148220 | MS.gene93743:intron | 35.0% | |
| TGAATGTTTAACAGTCTCTC+AGG | + | chr4.2:148833-148852 | None:intergenic | 35.0% | |
| TGACTACGAAAGTAAAGTCA+TGG | - | chr4.2:148249-148268 | MS.gene93743:intron | 35.0% | |
| ! | ATTTTCCAATTACCACACAC+AGG | - | chr4.2:148023-148042 | MS.gene93743:intron | 35.0% |
| ACAACATTTCCAGCTTGTAG+TGG | + | chr4.2:148133-148152 | None:intergenic | 40.0% | |
| AGGCACCATTTGGATATTCA+AGG | - | chr4.2:147907-147926 | MS.gene93743:intron | 40.0% | |
| CAAAGAATAACAAGAAGCCC+AGG | + | chr4.2:148321-148340 | None:intergenic | 40.0% | |
| CTACGAAAGTAAAGTCATGG+AGG | - | chr4.2:148252-148271 | MS.gene93743:intron | 40.0% | |
| CTCTATTGAATCTGCAGAAG+CGG | - | chr4.2:148993-149012 | MS.gene93743:intron | 40.0% | |
| CTTGGCATTGCCTTAAAATC+CGG | - | chr4.2:147966-147985 | MS.gene93743:intron | 40.0% | |
| TGAAATGTGGTGTCCCAAAA+AGG | - | chr4.2:148396-148415 | MS.gene93743:intron | 40.0% | |
| ! | TACCTCAAGTCCGGATTTTA+AGG | + | chr4.2:147979-147998 | None:intergenic | 40.0% |
| ! | TTACAGACTAAGGCACCATT+TGG | - | chr4.2:147897-147916 | MS.gene93743:intron | 40.0% |
| ! | TTGTAGCTCTGCTTCTGTTA+GGG | + | chr4.2:149026-149045 | None:intergenic | 40.0% |
| !! | AGCTTGTAGTGGATCAAATC+CGG | + | chr4.2:148122-148141 | None:intergenic | 40.0% |
| !!! | GTTCAACTTGTGCTGGTTTT+GGG | - | chr4.2:148151-148170 | MS.gene93743:intron | 40.0% |
| !!! | TGTTCAACTTGTGCTGGTTT+TGG | - | chr4.2:148150-148169 | MS.gene93743:intron | 40.0% |
| !!! | TGTTTTGGCCAGAGATAATC+AGG | - | chr4.2:148490-148509 | MS.gene93743:intron | 40.0% |
| AAGTGCCTGTGTGTGGTAAT+TGG | + | chr4.2:148031-148050 | None:intergenic | 45.0% | |
| ACTTACGGCCTGATTATCTC+TGG | + | chr4.2:148501-148520 | None:intergenic | 45.0% | |
| AGTACTGCTTCAAGAAGTCG+AGG | - | chr4.2:149045-149064 | MS.gene93743:CDS | 45.0% | |
| CAAACATAAGTGCCTGTGTG+TGG | + | chr4.2:148038-148057 | None:intergenic | 45.0% | |
| CGCTTCTGCAGATTCAATAG+AGG | + | chr4.2:148995-149014 | None:intergenic | 45.0% | |
| GAGTTGAGATACCTCAAGTC+CGG | + | chr4.2:147988-148007 | None:intergenic | 45.0% | |
| GGATTTGATCCACTACAAGC+TGG | - | chr4.2:148121-148140 | MS.gene93743:intron | 45.0% | |
| TATAAACTTGGGTCTGCAGC+AGG | - | chr4.2:148921-148940 | MS.gene93743:CDS | 45.0% | |
| TGCCTTAAAATCCGGACTTG+AGG | - | chr4.2:147974-147993 | MS.gene93743:intron | 45.0% | |
| TTGAGATCCATCCAACAGTG+AGG | + | chr4.2:148177-148196 | None:intergenic | 45.0% | |
| ! | CTTGTAGCTCTGCTTCTGTT+AGG | + | chr4.2:149027-149046 | None:intergenic | 45.0% |
| ! | GGACTTGGAGTTACTTTCCT+TGG | - | chr4.2:147948-147967 | MS.gene93743:intron | 45.0% |
| !! | AGTCTCTCAGGGTTGATACA+TGG | + | chr4.2:148821-148840 | None:intergenic | 45.0% |
| !!! | CGGATTTTAAGGCAATGCCA+AGG | + | chr4.2:147968-147987 | None:intergenic | 45.0% |
| ! | AGCAGAGAGCGCAAGAACTT+TGG | + | chr4.2:148584-148603 | None:intergenic | 50.0% |
| ! | TTTTGGGCCTCACTGTTGGA+TGG | - | chr4.2:148167-148186 | MS.gene93743:intron | 50.0% |
| !!! | CTGGTTTTGGGCCTCACTGT+TGG | - | chr4.2:148163-148182 | MS.gene93743:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 147774 | 149084 | 147774 | ID=MS.gene93743 |
| chr4.2 | mRNA | 147774 | 149084 | 147774 | ID=MS.gene93743.t1;Parent=MS.gene93743 |
| chr4.2 | exon | 149034 | 149084 | 149034 | ID=MS.gene93743.t1.exon1;Parent=MS.gene93743.t1 |
| chr4.2 | CDS | 149034 | 149084 | 149034 | ID=cds.MS.gene93743.t1;Parent=MS.gene93743.t1 |
| chr4.2 | exon | 148863 | 148955 | 148863 | ID=MS.gene93743.t1.exon2;Parent=MS.gene93743.t1 |
| chr4.2 | CDS | 148863 | 148955 | 148863 | ID=cds.MS.gene93743.t1;Parent=MS.gene93743.t1 |
| chr4.2 | exon | 148585 | 148743 | 148585 | ID=MS.gene93743.t1.exon3;Parent=MS.gene93743.t1 |
| chr4.2 | CDS | 148585 | 148743 | 148585 | ID=cds.MS.gene93743.t1;Parent=MS.gene93743.t1 |
| chr4.2 | exon | 147774 | 147848 | 147774 | ID=MS.gene93743.t1.exon4;Parent=MS.gene93743.t1 |
| chr4.2 | CDS | 147774 | 147848 | 147774 | ID=cds.MS.gene93743.t1;Parent=MS.gene93743.t1 |
| Gene Sequence |
| Protein sequence |
