Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94035.t1 | GAU40887.1 | 89.8 | 324 | 30 | 1 | 1 | 324 | 1 | 321 | 2.40E-169 | 604.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94035.t1 | E7C196 | 61.8 | 322 | 113 | 2 | 9 | 323 | 8 | 326 | 2.3e-115 | 416.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94035.t1 | A0A2Z6PC20 | 89.8 | 324 | 30 | 1 | 1 | 324 | 1 | 321 | 1.8e-169 | 604.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene94035 | 0.819824 | 9.48E-53 | -1.69E-46 |
| MS.gene049356 | MS.gene94035 | 0.833892 | 4.11E-56 | -1.69E-46 |
| MS.gene049369 | MS.gene94035 | 0.845104 | 5.00E-59 | -1.69E-46 |
| MS.gene049396 | MS.gene94035 | 0.858928 | 5.88E-63 | -1.69E-46 |
| MS.gene049609 | MS.gene94035 | 0.822899 | 1.85E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene94035 | 0.816522 | 5.30E-52 | -1.69E-46 |
| MS.gene050593 | MS.gene94035 | 0.814763 | 1.31E-51 | -1.69E-46 |
| MS.gene050670 | MS.gene94035 | 0.858868 | 6.13E-63 | -1.69E-46 |
| MS.gene050747 | MS.gene94035 | 0.831057 | 2.07E-55 | -1.69E-46 |
| MS.gene050772 | MS.gene94035 | 0.819604 | 1.06E-52 | -1.69E-46 |
| MS.gene05080 | MS.gene94035 | 0.800642 | 1.31E-48 | -1.69E-46 |
| MS.gene05081 | MS.gene94035 | 0.816276 | 6.01E-52 | -1.69E-46 |
| MS.gene050950 | MS.gene94035 | 0.820699 | 5.97E-53 | -1.69E-46 |
| MS.gene05105 | MS.gene94035 | 0.851138 | 1.08E-60 | -1.69E-46 |
| MS.gene051387 | MS.gene94035 | 0.832898 | 7.27E-56 | -1.69E-46 |
| MS.gene051525 | MS.gene94035 | 0.845263 | 4.53E-59 | -1.69E-46 |
| MS.gene051605 | MS.gene94035 | 0.80073 | 1.26E-48 | -1.69E-46 |
| MS.gene051838 | MS.gene94035 | 0.817126 | 3.88E-52 | -1.69E-46 |
| MS.gene051874 | MS.gene94035 | 0.826433 | 2.72E-54 | -1.69E-46 |
| MS.gene051941 | MS.gene94035 | 0.805291 | 1.43E-49 | -1.69E-46 |
| MS.gene052136 | MS.gene94035 | 0.810945 | 8.95E-51 | -1.69E-46 |
| MS.gene052188 | MS.gene94035 | 0.813117 | 3.01E-51 | -1.69E-46 |
| MS.gene052346 | MS.gene94035 | 0.849881 | 2.43E-60 | -1.69E-46 |
| MS.gene052414 | MS.gene94035 | 0.852826 | 3.58E-61 | -1.69E-46 |
| MS.gene052421 | MS.gene94035 | 0.867441 | 1.37E-65 | -1.69E-46 |
| MS.gene052485 | MS.gene94035 | 0.81409 | 1.84E-51 | -1.69E-46 |
| MS.gene052501 | MS.gene94035 | 0.804793 | 1.82E-49 | -1.69E-46 |
| MS.gene052505 | MS.gene94035 | 0.811452 | 6.95E-51 | -1.69E-46 |
| MS.gene052982 | MS.gene94035 | 0.829022 | 6.50E-55 | -1.69E-46 |
| MS.gene052999 | MS.gene94035 | 0.803106 | 4.08E-49 | -1.69E-46 |
| MS.gene053103 | MS.gene94035 | 0.802722 | 4.90E-49 | -1.69E-46 |
| MS.gene053169 | MS.gene94035 | 0.808447 | 3.08E-50 | -1.69E-46 |
| MS.gene05335 | MS.gene94035 | 0.834956 | 2.22E-56 | -1.69E-46 |
| MS.gene053449 | MS.gene94035 | 0.84329 | 1.53E-58 | -1.69E-46 |
| MS.gene053574 | MS.gene94035 | 0.839526 | 1.50E-57 | -1.69E-46 |
| MS.gene053626 | MS.gene94035 | 0.821916 | 3.13E-53 | -1.69E-46 |
| MS.gene053758 | MS.gene94035 | 0.802853 | 4.61E-49 | -1.69E-46 |
| MS.gene053824 | MS.gene94035 | 0.818728 | 1.68E-52 | -1.69E-46 |
| MS.gene053889 | MS.gene94035 | 0.804272 | 2.34E-49 | -1.69E-46 |
| MS.gene053912 | MS.gene94035 | 0.849448 | 3.21E-60 | -1.69E-46 |
| MS.gene053916 | MS.gene94035 | 0.828308 | 9.66E-55 | -1.69E-46 |
| MS.gene054408 | MS.gene94035 | 0.81372 | 2.22E-51 | -1.69E-46 |
| MS.gene054556 | MS.gene94035 | 0.800468 | 1.42E-48 | -1.69E-46 |
| MS.gene05494 | MS.gene94035 | 0.843405 | 1.43E-58 | -1.69E-46 |
| MS.gene05505 | MS.gene94035 | 0.86464 | 1.05E-64 | -1.69E-46 |
| MS.gene055116 | MS.gene94035 | 0.81097 | 8.84E-51 | -1.69E-46 |
| MS.gene055224 | MS.gene94035 | 0.843354 | 1.48E-58 | -1.69E-46 |
| MS.gene055289 | MS.gene94035 | 0.81221 | 4.75E-51 | -1.69E-46 |
| MS.gene055369 | MS.gene94035 | 0.80728 | 5.46E-50 | -1.69E-46 |
| MS.gene055741 | MS.gene94035 | 0.870699 | 1.20E-66 | -1.69E-46 |
| MS.gene055744 | MS.gene94035 | 0.870844 | 1.07E-66 | -1.69E-46 |
| MS.gene055909 | MS.gene94035 | 0.859501 | 3.96E-63 | -1.69E-46 |
| MS.gene055911 | MS.gene94035 | 0.858591 | 7.42E-63 | -1.69E-46 |
| MS.gene056166 | MS.gene94035 | 0.846856 | 1.67E-59 | -1.69E-46 |
| MS.gene056422 | MS.gene94035 | 0.859216 | 4.82E-63 | -1.69E-46 |
| MS.gene056448 | MS.gene94035 | 0.800482 | 1.41E-48 | -1.69E-46 |
| MS.gene056511 | MS.gene94035 | 0.867174 | 1.66E-65 | -1.69E-46 |
| MS.gene056514 | MS.gene94035 | 0.800202 | 1.61E-48 | -1.69E-46 |
| MS.gene057019 | MS.gene94035 | 0.834664 | 2.63E-56 | -1.69E-46 |
| MS.gene057076 | MS.gene94035 | 0.822045 | 2.92E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene94035 | 0.827749 | 1.32E-54 | -1.69E-46 |
| MS.gene057289 | MS.gene94035 | 0.819322 | 1.23E-52 | -1.69E-46 |
| MS.gene057734 | MS.gene94035 | 0.838862 | 2.23E-57 | -1.69E-46 |
| MS.gene057823 | MS.gene94035 | 0.806622 | 7.52E-50 | -1.69E-46 |
| MS.gene058007 | MS.gene94035 | 0.823192 | 1.58E-53 | -1.69E-46 |
| MS.gene058075 | MS.gene94035 | 0.820498 | 6.65E-53 | -1.69E-46 |
| MS.gene058120 | MS.gene94035 | 0.864404 | 1.25E-64 | -1.69E-46 |
| MS.gene058130 | MS.gene94035 | 0.818549 | 1.85E-52 | -1.69E-46 |
| MS.gene058376 | MS.gene94035 | 0.849422 | 3.27E-60 | -1.69E-46 |
| MS.gene05837 | MS.gene94035 | 0.824169 | 9.34E-54 | -1.69E-46 |
| MS.gene058444 | MS.gene94035 | 0.810259 | 1.26E-50 | -1.69E-46 |
| MS.gene058700 | MS.gene94035 | 0.860186 | 2.46E-63 | -1.69E-46 |
| MS.gene058928 | MS.gene94035 | 0.816105 | 6.56E-52 | -1.69E-46 |
| MS.gene058948 | MS.gene94035 | 0.82438 | 8.33E-54 | -1.69E-46 |
| MS.gene059305 | MS.gene94035 | 0.814469 | 1.52E-51 | -1.69E-46 |
| MS.gene059366 | MS.gene94035 | 0.828324 | 9.58E-55 | -1.69E-46 |
| MS.gene059368 | MS.gene94035 | 0.819691 | 1.02E-52 | -1.69E-46 |
| MS.gene059420 | MS.gene94035 | 0.833735 | 4.50E-56 | -1.69E-46 |
| MS.gene059455 | MS.gene94035 | 0.815228 | 1.03E-51 | -1.69E-46 |
| MS.gene059667 | MS.gene94035 | 0.827066 | 1.92E-54 | -1.69E-46 |
| MS.gene059674 | MS.gene94035 | 0.8143 | 1.65E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene94035 | 0.827339 | 1.65E-54 | -1.69E-46 |
| MS.gene059711 | MS.gene94035 | 0.829462 | 5.08E-55 | -1.69E-46 |
| MS.gene059807 | MS.gene94035 | 0.869994 | 2.04E-66 | -1.69E-46 |
| MS.gene060372 | MS.gene94035 | 0.886057 | 4.76E-72 | -1.69E-46 |
| MS.gene060401 | MS.gene94035 | 0.822762 | 1.99E-53 | -1.69E-46 |
| MS.gene060403 | MS.gene94035 | 0.806934 | 6.46E-50 | -1.69E-46 |
| MS.gene060835 | MS.gene94035 | 0.83398 | 3.90E-56 | -1.69E-46 |
| MS.gene060837 | MS.gene94035 | 0.868755 | 5.16E-66 | -1.69E-46 |
| MS.gene06097 | MS.gene94035 | 0.845414 | 4.12E-59 | -1.69E-46 |
| MS.gene061154 | MS.gene94035 | 0.856786 | 2.54E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94035.t1 | MTR_4g072350 | 81.790 | 324 | 56 | 1 | 1 | 324 | 1 | 321 | 0.0 | 548 |
| MS.gene94035.t1 | MTR_4g072360 | 77.709 | 323 | 69 | 1 | 1 | 323 | 1 | 320 | 0.0 | 528 |
| MS.gene94035.t1 | MTR_4g072320 | 78.019 | 323 | 62 | 3 | 1 | 323 | 1 | 314 | 0.0 | 520 |
| MS.gene94035.t1 | MTR_6g073110 | 59.502 | 321 | 127 | 1 | 1 | 321 | 1 | 318 | 1.17e-146 | 416 |
| MS.gene94035.t1 | MTR_0374s0050 | 50.920 | 326 | 146 | 3 | 1 | 323 | 1 | 315 | 6.11e-112 | 327 |
| MS.gene94035.t1 | MTR_3g083130 | 49.844 | 321 | 138 | 8 | 8 | 321 | 9 | 313 | 6.98e-102 | 302 |
| MS.gene94035.t1 | MTR_4g036845 | 47.352 | 321 | 159 | 7 | 5 | 323 | 2 | 314 | 2.20e-93 | 280 |
| MS.gene94035.t1 | MTR_5g097900 | 50.485 | 309 | 134 | 7 | 16 | 321 | 19 | 311 | 6.07e-92 | 276 |
| MS.gene94035.t1 | MTR_5g097910 | 52.265 | 287 | 127 | 5 | 16 | 300 | 19 | 297 | 1.30e-91 | 276 |
| MS.gene94035.t1 | MTR_5g097940 | 52.265 | 287 | 127 | 5 | 16 | 300 | 19 | 297 | 1.30e-91 | 276 |
| MS.gene94035.t1 | MTR_8g070095 | 46.082 | 319 | 156 | 7 | 11 | 323 | 10 | 318 | 1.51e-88 | 268 |
| MS.gene94035.t1 | MTR_8g070115 | 42.547 | 322 | 175 | 5 | 3 | 323 | 2 | 314 | 3.29e-82 | 252 |
| MS.gene94035.t1 | MTR_6g072650 | 80.000 | 150 | 8 | 1 | 175 | 324 | 10 | 137 | 5.60e-82 | 244 |
| MS.gene94035.t1 | MTR_4g021350 | 43.810 | 315 | 163 | 7 | 11 | 323 | 10 | 312 | 1.16e-79 | 245 |
| MS.gene94035.t1 | MTR_7g063580 | 43.425 | 327 | 171 | 7 | 1 | 323 | 37 | 353 | 3.29e-78 | 243 |
| MS.gene94035.t1 | MTR_4g021410 | 42.547 | 322 | 164 | 7 | 1 | 321 | 1 | 302 | 1.91e-73 | 229 |
| MS.gene94035.t1 | MTR_4g021350 | 44.444 | 288 | 147 | 6 | 11 | 297 | 10 | 285 | 9.41e-72 | 224 |
| MS.gene94035.t1 | MTR_4g021450 | 41.667 | 324 | 168 | 7 | 1 | 323 | 1 | 304 | 7.50e-71 | 222 |
| MS.gene94035.t1 | MTR_4g021410 | 44.286 | 280 | 138 | 6 | 43 | 321 | 3 | 265 | 3.06e-68 | 214 |
| MS.gene94035.t1 | MTR_7g070500 | 37.898 | 314 | 180 | 7 | 11 | 321 | 17 | 318 | 3.58e-67 | 213 |
| MS.gene94035.t1 | MTR_7g070500 | 37.671 | 292 | 170 | 5 | 11 | 302 | 17 | 296 | 3.03e-61 | 197 |
| MS.gene94035.t1 | MTR_4g072060 | 38.127 | 299 | 166 | 8 | 9 | 297 | 3 | 292 | 1.63e-56 | 186 |
| MS.gene94035.t1 | MTR_4g021410 | 44.215 | 242 | 118 | 5 | 81 | 321 | 1 | 226 | 1.34e-55 | 181 |
| MS.gene94035.t1 | MTR_4g021450 | 43.033 | 244 | 122 | 5 | 81 | 323 | 1 | 228 | 3.11e-53 | 174 |
| MS.gene94035.t1 | MTR_4g021450 | 46.111 | 180 | 92 | 2 | 145 | 323 | 10 | 185 | 1.19e-49 | 164 |
| MS.gene94035.t1 | MTR_6g072710 | 63.717 | 113 | 41 | 0 | 193 | 305 | 1 | 113 | 1.32e-48 | 159 |
| MS.gene94035.t1 | MTR_6g072705 | 93.478 | 46 | 3 | 0 | 110 | 155 | 40 | 85 | 1.09e-25 | 98.6 |
| MS.gene94035.t1 | MTR_3g449790 | 25.260 | 289 | 155 | 10 | 42 | 295 | 83 | 345 | 1.27e-11 | 65.1 |
| MS.gene94035.t1 | MTR_3g092140 | 23.841 | 302 | 174 | 10 | 32 | 297 | 69 | 350 | 3.02e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94035.t1 | AT1G59960 | 51.623 | 308 | 145 | 2 | 17 | 323 | 21 | 325 | 1.44e-118 | 345 |
| MS.gene94035.t1 | AT1G59950 | 51.299 | 308 | 146 | 2 | 17 | 323 | 15 | 319 | 2.88e-109 | 321 |
| MS.gene94035.t1 | AT2G37790 | 48.408 | 314 | 151 | 5 | 11 | 323 | 10 | 313 | 1.19e-96 | 288 |
| MS.gene94035.t1 | AT2G37770 | 47.988 | 323 | 156 | 6 | 3 | 323 | 2 | 314 | 1.02e-94 | 284 |
| MS.gene94035.t1 | AT3G53880 | 45.860 | 314 | 160 | 5 | 11 | 323 | 10 | 314 | 1.15e-88 | 268 |
| MS.gene94035.t1 | AT2G37760 | 45.541 | 314 | 157 | 5 | 11 | 323 | 10 | 310 | 5.04e-86 | 261 |
| MS.gene94035.t1 | AT2G37760 | 45.541 | 314 | 157 | 5 | 11 | 323 | 10 | 310 | 5.04e-86 | 261 |
| MS.gene94035.t1 | AT5G62420 | 43.987 | 316 | 166 | 8 | 11 | 323 | 8 | 315 | 3.78e-85 | 259 |
| MS.gene94035.t1 | AT2G37760 | 45.675 | 289 | 144 | 4 | 11 | 299 | 10 | 285 | 2.14e-79 | 244 |
| MS.gene94035.t1 | AT2G37760 | 45.172 | 290 | 146 | 4 | 11 | 300 | 10 | 286 | 4.83e-79 | 243 |
| MS.gene94035.t1 | AT2G37760 | 45.172 | 290 | 146 | 4 | 11 | 300 | 10 | 286 | 4.83e-79 | 243 |
| MS.gene94035.t1 | AT3G53880 | 45.296 | 287 | 148 | 4 | 11 | 297 | 10 | 287 | 5.52e-78 | 241 |
| MS.gene94035.t1 | AT5G01670 | 39.938 | 323 | 179 | 8 | 2 | 321 | 9 | 319 | 3.14e-77 | 239 |
| MS.gene94035.t1 | AT5G01670 | 36.857 | 350 | 179 | 9 | 2 | 321 | 9 | 346 | 2.69e-71 | 225 |
| MS.gene94035.t1 | AT2G37760 | 46.008 | 263 | 135 | 4 | 62 | 323 | 8 | 264 | 1.50e-70 | 220 |
| MS.gene94035.t1 | AT2G37760 | 46.008 | 263 | 135 | 4 | 62 | 323 | 8 | 264 | 1.50e-70 | 220 |
| MS.gene94035.t1 | AT2G21260 | 39.000 | 300 | 164 | 8 | 8 | 297 | 2 | 292 | 4.00e-64 | 205 |
| MS.gene94035.t1 | AT2G21250 | 38.000 | 300 | 167 | 8 | 8 | 297 | 2 | 292 | 1.43e-62 | 201 |
| MS.gene94035.t1 | AT2G37770 | 50.000 | 212 | 95 | 5 | 3 | 213 | 2 | 203 | 1.49e-59 | 192 |
| MS.gene94035.t1 | AT2G21250 | 38.589 | 241 | 129 | 8 | 8 | 238 | 2 | 233 | 1.30e-45 | 155 |
| MS.gene94035.t1 | AT2G21260 | 36.036 | 222 | 127 | 6 | 86 | 297 | 8 | 224 | 2.10e-39 | 139 |
| MS.gene94035.t1 | AT1G06690 | 26.298 | 289 | 158 | 10 | 42 | 295 | 94 | 362 | 4.21e-14 | 72.8 |
Find 73 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGGAGCATACAAAGTATTT+TGG | 0.154726 | 6.4:-49713467 | MS.gene94035:CDS |
| TGAAGCTCTCACTTCAATTT+TGG | 0.164436 | 6.4:-49714234 | MS.gene94035:CDS |
| TTTGATACAGCTTCTATCTA+TGG | 0.288397 | 6.4:-49714182 | MS.gene94035:CDS |
| GCAGTTCCTAGGCCTATTAT+TGG | 0.318436 | 6.4:+49714278 | None:intergenic |
| TTGGGGTTCTAATGCTGTTA+TGG | 0.341183 | 6.4:-49713448 | MS.gene94035:CDS |
| GTTACTAACACCAATAGATT+TGG | 0.354709 | 6.4:+49713768 | None:intergenic |
| GAATCTGAACCAAGAAGAAT+TGG | 0.366890 | 6.4:-49712880 | MS.gene94035:CDS |
| TATGGAGAATCCTATTCTTC+AGG | 0.366943 | 6.4:-49713430 | MS.gene94035:CDS |
| GGGGAAAAGATGCCAATAAT+AGG | 0.375376 | 6.4:-49714290 | MS.gene94035:CDS |
| TCTATTGGTGTTAGTAACTT+TGG | 0.376784 | 6.4:-49713763 | MS.gene94035:CDS |
| ATGGAAGATTGTTACAGATT+AGG | 0.378605 | 6.4:-49713796 | MS.gene94035:CDS |
| AGGGAATTCTGCATGAATAA+AGG | 0.386783 | 6.4:-49713516 | MS.gene94035:CDS |
| GAAGTGAGAGCTTCATTTGA+TGG | 0.392979 | 6.4:+49714242 | None:intergenic |
| CATGAGGTGATACTAAATTC+AGG | 0.395724 | 6.4:-49714311 | MS.gene94035:CDS |
| TCAGCAATTTCCTGAAGAAT+AGG | 0.400823 | 6.4:+49713420 | None:intergenic |
| CCATGTTCCTTTGATGTCAA+AGG | 0.403046 | 6.4:+49713825 | None:intergenic |
| TTAGGCTTAGCCAAATCTAT+TGG | 0.410561 | 6.4:-49713778 | MS.gene94035:CDS |
| AAAATCTGGAAATATTTGAT+TGG | 0.413557 | 6.4:-49712902 | MS.gene94035:CDS |
| CATGTTCCTTTGATGTCAAA+GGG | 0.424119 | 6.4:+49713826 | None:intergenic |
| AGAGAGGATGAAACAAAATC+TGG | 0.435542 | 6.4:-49712916 | MS.gene94035:CDS |
| TCTTGCAGATAGCACTAAGA+TGG | 0.451601 | 6.4:-49712983 | MS.gene94035:intron |
| AAATGGACCTTACAAATCCT+TGG | 0.461855 | 6.4:-49712799 | MS.gene94035:CDS |
| AGCTTGGCCAAGAGGTTCTT+CGG | 0.463692 | 6.4:+49714157 | None:intergenic |
| TCCACAATGCAGACTTTACA+AGG | 0.465814 | 6.4:-49712841 | MS.gene94035:CDS |
| GAGGAGTTGTAGCAGTTCCT+AGG | 0.470932 | 6.4:+49714267 | None:intergenic |
| TGGATCTCTACTTGATTCAC+TGG | 0.472213 | 6.4:-49713906 | MS.gene94035:CDS |
| AGCATCAGTGCACCATAGCT+TGG | 0.473444 | 6.4:+49714076 | None:intergenic |
| AGCTCTTCCAAGGATTTGTA+AGG | 0.476136 | 6.4:+49712792 | None:intergenic |
| ATTGTTTAGGAATCTGAGGT+TGG | 0.476463 | 6.4:-49713935 | MS.gene94035:intron |
| TAGAAAGAAGAGTGTAGCTC+AGG | 0.477815 | 6.4:-49713394 | MS.gene94035:intron |
| AGAGCTTCATTTGATGGAAG+AGG | 0.479484 | 6.4:+49714248 | None:intergenic |
| GTTGAAGGCTTTAACTTCAC+AGG | 0.480998 | 6.4:-49713862 | MS.gene94035:CDS |
| ACATCTTCTTTCAATCTCAC+TGG | 0.482395 | 6.4:+49713883 | None:intergenic |
| GAATCTGAGGTTGGAGTATG+TGG | 0.495354 | 6.4:-49713926 | MS.gene94035:CDS |
| TGTGTGTGCATGGTCACCAT+TGG | 0.497595 | 6.4:-49713487 | MS.gene94035:CDS |
| AGGCTTTAACTTCACAGGTA+AGG | 0.505666 | 6.4:-49713857 | MS.gene94035:CDS |
| GTGTGTGCATGGTCACCATT+GGG | 0.511118 | 6.4:-49713486 | MS.gene94035:CDS |
| ATGAGGTGATACTAAATTCA+GGG | 0.518353 | 6.4:-49714310 | MS.gene94035:CDS |
| AGGTAGAAATGAATCCATCT+TGG | 0.519132 | 6.4:-49713554 | MS.gene94035:CDS |
| AGAGAACAATGCTTGCTTGA+GGG | 0.524372 | 6.4:+49714019 | None:intergenic |
| AAGATGCCAATAATAGGCCT+AGG | 0.525430 | 6.4:-49714284 | MS.gene94035:CDS |
| CAAATCCTTGGAAGAGCTGT+GGG | 0.525762 | 6.4:-49712787 | MS.gene94035:CDS |
| CAAAGGAACATGGAAAGCTA+TGG | 0.532610 | 6.4:-49713815 | MS.gene94035:CDS |
| TCTTATACCTGATTAACTGC+AGG | 0.540231 | 6.4:+49713697 | None:intergenic |
| CCTTTGACATCAAAGGAACA+TGG | 0.540695 | 6.4:-49713825 | MS.gene94035:CDS |
| GTGATTCCCTTTGACATCAA+AGG | 0.542697 | 6.4:-49713832 | MS.gene94035:CDS |
| AGATTGAAAGAAGATGTTGA+AGG | 0.547100 | 6.4:-49713877 | MS.gene94035:CDS |
| CTTGGCAGCAAGGAAAACTC+AGG | 0.549272 | 6.4:-49713536 | MS.gene94035:CDS |
| TATGGTACCGAAGAACCTCT+TGG | 0.552223 | 6.4:-49714164 | MS.gene94035:CDS |
| GTGTCAAAAGCTTTAGAGCT+CGG | 0.554884 | 6.4:-49714134 | MS.gene94035:CDS |
| TCACCATGACCTTGTTCTCA+AGG | 0.559342 | 6.4:-49714054 | MS.gene94035:CDS |
| ATGAATCCATCTTGGCAGCA+AGG | 0.561015 | 6.4:-49713546 | MS.gene94035:CDS |
| AATGTCTGTAACCAATGTCA+AGG | 0.577476 | 6.4:+49714204 | None:intergenic |
| TTGGTTGATGCCCTTGACAT+TGG | 0.578089 | 6.4:-49714215 | MS.gene94035:CDS |
| ACCATCCCACAGCTCTTCCA+AGG | 0.580081 | 6.4:+49712782 | None:intergenic |
| GAACAATGCTTGCTTGAGGG+TGG | 0.589137 | 6.4:+49714022 | None:intergenic |
| ACAAATCCTTGGAAGAGCTG+TGG | 0.595019 | 6.4:-49712788 | MS.gene94035:CDS |
| TGAAAAGCTTCAACAAAGAG+AGG | 0.595864 | 6.4:-49712932 | MS.gene94035:CDS |
| GAGAGAACAATGCTTGCTTG+AGG | 0.603868 | 6.4:+49714018 | None:intergenic |
| TGGAATTGTTTAGGAATCTG+AGG | 0.606116 | 6.4:-49713939 | MS.gene94035:intron |
| GGAAGCAAATAAACTTCATG+AGG | 0.606465 | 6.4:-49714327 | MS.gene94035:CDS |
| ATGTCTGTAACCAATGTCAA+GGG | 0.608239 | 6.4:+49714205 | None:intergenic |
| AAGGAATTCATGTGTGTGCA+TGG | 0.617297 | 6.4:-49713497 | MS.gene94035:CDS |
| TTTGACACAGCTTGGCCAAG+AGG | 0.617936 | 6.4:+49714149 | None:intergenic |
| AATACTTTGTATGCTCCCAA+TGG | 0.618795 | 6.4:+49713471 | None:intergenic |
| TATTCTTCAGGAAATTGCTG+AGG | 0.632039 | 6.4:-49713418 | MS.gene94035:CDS |
| GAGCCTTGAGAACAAGGTCA+TGG | 0.636860 | 6.4:+49714051 | None:intergenic |
| GCCTTGTAAAGTCTGCATTG+TGG | 0.641275 | 6.4:+49712840 | None:intergenic |
| CTAAGATGGATACATGAGCA+AGG | 0.646695 | 6.4:-49712969 | MS.gene94035:CDS |
| TTGGCAGCAAGGAAAACTCA+GGG | 0.649378 | 6.4:-49713535 | MS.gene94035:CDS |
| TGAGGTGATACTAAATTCAG+GGG | 0.660799 | 6.4:-49714309 | MS.gene94035:CDS |
| TAAGATGGATACATGAGCAA+GGG | 0.664827 | 6.4:-49712968 | MS.gene94035:CDS |
| TCCTTGGAAGAGCTGTGGGA+TGG | 0.668107 | 6.4:-49712783 | MS.gene94035:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATCTGGAAATATTTGAT+TGG | - | chr6.4:49714200-49714219 | MS.gene94035:CDS | 20.0% |
| !!! | AATTCTGTATCATTTTTTGT+TGG | - | chr6.4:49713143-49713162 | MS.gene94035:intron | 20.0% |
| !!! | ATTTTTTGTTGGAATTGTTT+AGG | - | chr6.4:49713154-49713173 | MS.gene94035:intron | 20.0% |
| !!! | GAAATGTTTTTATCTGAAAA+TGG | - | chr6.4:49714286-49714305 | MS.gene94035:CDS | 20.0% |
| ! | AACCTTAAAATGCTAGATAA+AGG | - | chr6.4:49713805-49713824 | MS.gene94035:CDS | 25.0% |
| ! | ACCTTAAAATGCTAGATAAA+GGG | - | chr6.4:49713806-49713825 | MS.gene94035:CDS | 25.0% |
| ! | AGCATTAACGTAATATTGAT+TGG | + | chr6.4:49713473-49713492 | None:intergenic | 25.0% |
| ! | TCAAAGTTGTAACTGAAATT+AGG | + | chr6.4:49713756-49713775 | None:intergenic | 25.0% |
| ! | TGTTATGAAATTCACTCAAT+TGG | - | chr6.4:49713502-49713521 | MS.gene94035:CDS | 25.0% |
| !! | ACTTATGCATAAGTACTTTT+AGG | - | chr6.4:49714027-49714046 | MS.gene94035:CDS | 25.0% |
| !! | ATATTGATTGGTTAATGACT+CGG | + | chr6.4:49713461-49713480 | None:intergenic | 25.0% |
| !!! | GGTTTTAGAACAATTTGTTT+AGG | + | chr6.4:49713789-49713808 | None:intergenic | 25.0% |
| AGATTGAAAGAAGATGTTGA+AGG | - | chr6.4:49713225-49713244 | MS.gene94035:intron | 30.0% | |
| ATGAGGTGATACTAAATTCA+GGG | - | chr6.4:49712792-49712811 | MS.gene94035:CDS | 30.0% | |
| ATGGAAGATTGTTACAGATT+AGG | - | chr6.4:49713306-49713325 | MS.gene94035:intron | 30.0% | |
| GTTACTAACACCAATAGATT+TGG | + | chr6.4:49713337-49713356 | None:intergenic | 30.0% | |
| TTTGATACAGCTTCTATCTA+TGG | - | chr6.4:49712920-49712939 | MS.gene94035:CDS | 30.0% | |
| ! | TGATTTTGTCCAATTCTTCT+TGG | + | chr6.4:49714234-49714253 | None:intergenic | 30.0% |
| ! | TTTTCATTACTTCCAAGCTA+TGG | - | chr6.4:49713014-49713033 | MS.gene94035:intron | 30.0% |
| !! | CTCTCGAACTTTTTCTTTTA+AGG | - | chr6.4:49713102-49713121 | MS.gene94035:intron | 30.0% |
| !! | TCTATTGGTGTTAGTAACTT+TGG | - | chr6.4:49713339-49713358 | MS.gene94035:intron | 30.0% |
| AAATGGACCTTACAAATCCT+TGG | - | chr6.4:49714303-49714322 | MS.gene94035:CDS | 35.0% | |
| AATACTTTGTATGCTCCCAA+TGG | + | chr6.4:49713634-49713653 | None:intergenic | 35.0% | |
| AATGTCTGTAACCAATGTCA+AGG | + | chr6.4:49712901-49712920 | None:intergenic | 35.0% | |
| ACATCTTCTTTCAATCTCAC+TGG | + | chr6.4:49713222-49713241 | None:intergenic | 35.0% | |
| AGAGAGGATGAAACAAAATC+TGG | - | chr6.4:49714186-49714205 | MS.gene94035:CDS | 35.0% | |
| AGGGAATTCTGCATGAATAA+AGG | - | chr6.4:49713586-49713605 | MS.gene94035:intron | 35.0% | |
| AGGTAGAAATGAATCCATCT+TGG | - | chr6.4:49713548-49713567 | MS.gene94035:CDS | 35.0% | |
| ATGTCTGTAACCAATGTCAA+GGG | + | chr6.4:49712900-49712919 | None:intergenic | 35.0% | |
| CATGAGGTGATACTAAATTC+AGG | - | chr6.4:49712791-49712810 | MS.gene94035:CDS | 35.0% | |
| CATGTTCCTTTGATGTCAAA+GGG | + | chr6.4:49713279-49713298 | None:intergenic | 35.0% | |
| CTACGAACATAAATACTTCG+AGG | - | chr6.4:49713857-49713876 | MS.gene94035:CDS | 35.0% | |
| CTGATTAACTGCAGGAAAAA+TGG | + | chr6.4:49713400-49713419 | None:intergenic | 35.0% | |
| GAATCTGAACCAAGAAGAAT+TGG | - | chr6.4:49714222-49714241 | MS.gene94035:CDS | 35.0% | |
| GGAAGCAAATAAACTTCATG+AGG | - | chr6.4:49712775-49712794 | MS.gene94035:CDS | 35.0% | |
| TAAGATGGATACATGAGCAA+GGG | - | chr6.4:49714134-49714153 | MS.gene94035:CDS | 35.0% | |
| TATGGAGAATCCTATTCTTC+AGG | - | chr6.4:49713672-49713691 | MS.gene94035:intron | 35.0% | |
| TATTCTTCAGGAAATTGCTG+AGG | - | chr6.4:49713684-49713703 | MS.gene94035:intron | 35.0% | |
| TCAGCAATTTCCTGAAGAAT+AGG | + | chr6.4:49713685-49713704 | None:intergenic | 35.0% | |
| TCTTATACCTGATTAACTGC+AGG | + | chr6.4:49713408-49713427 | None:intergenic | 35.0% | |
| TGAAAAGCTTCAACAAAGAG+AGG | - | chr6.4:49714170-49714189 | MS.gene94035:CDS | 35.0% | |
| TGAGGTGATACTAAATTCAG+GGG | - | chr6.4:49712793-49712812 | MS.gene94035:CDS | 35.0% | |
| TGGAATTGTTTAGGAATCTG+AGG | - | chr6.4:49713163-49713182 | MS.gene94035:intron | 35.0% | |
| TTAGGCTTAGCCAAATCTAT+TGG | - | chr6.4:49713324-49713343 | MS.gene94035:intron | 35.0% | |
| TTGCACAAAGTAATCCAAAC+AGG | + | chr6.4:49713832-49713851 | None:intergenic | 35.0% | |
| ! | ATTGTTTAGGAATCTGAGGT+TGG | - | chr6.4:49713167-49713186 | MS.gene94035:intron | 35.0% |
| ! | CATGTCGATTATCTCGATTT+AGG | - | chr6.4:49713528-49713547 | MS.gene94035:CDS | 35.0% |
| ! | CATTTTTCCTGCAGTTAATC+AGG | - | chr6.4:49713398-49713417 | MS.gene94035:CDS | 35.0% |
| ! | GCCCTTTATCTAGCATTTTA+AGG | + | chr6.4:49713810-49713829 | None:intergenic | 35.0% |
| ! | TAATGACTCGGACAAATACT+CGG | + | chr6.4:49713449-49713468 | None:intergenic | 35.0% |
| ! | TGAAGCTCTCACTTCAATTT+TGG | - | chr6.4:49712868-49712887 | MS.gene94035:CDS | 35.0% |
| !! | GGAGCATACAAAGTATTTTG+GGG | - | chr6.4:49713637-49713656 | MS.gene94035:intron | 35.0% |
| !! | GGGAGCATACAAAGTATTTT+GGG | - | chr6.4:49713636-49713655 | MS.gene94035:intron | 35.0% |
| !! | TGGGAGCATACAAAGTATTT+TGG | - | chr6.4:49713635-49713654 | MS.gene94035:intron | 35.0% |
| AAGATGCCAATAATAGGCCT+AGG | - | chr6.4:49712818-49712837 | MS.gene94035:CDS | 40.0% | |
| AAGGAATTCATGTGTGTGCA+TGG | - | chr6.4:49713605-49713624 | MS.gene94035:intron | 40.0% | |
| AGAGAACAATGCTTGCTTGA+GGG | + | chr6.4:49713086-49713105 | None:intergenic | 40.0% | |
| AGAGCTTCATTTGATGGAAG+AGG | + | chr6.4:49712857-49712876 | None:intergenic | 40.0% | |
| AGCTCTTCCAAGGATTTGTA+AGG | + | chr6.4:49714313-49714332 | None:intergenic | 40.0% | |
| CAAAAAGCTTTCCGTACTCT+TGG | - | chr6.4:49713365-49713384 | MS.gene94035:intron | 40.0% | |
| CAAAGGAACATGGAAAGCTA+TGG | - | chr6.4:49713287-49713306 | MS.gene94035:intron | 40.0% | |
| CCATGTTCCTTTGATGTCAA+AGG | + | chr6.4:49713280-49713299 | None:intergenic | 40.0% | |
| CCTTTGACATCAAAGGAACA+TGG | - | chr6.4:49713277-49713296 | MS.gene94035:intron | 40.0% | |
| CTAAGATGGATACATGAGCA+AGG | - | chr6.4:49714133-49714152 | MS.gene94035:CDS | 40.0% | |
| GAAGTGAGAGCTTCATTTGA+TGG | + | chr6.4:49712863-49712882 | None:intergenic | 40.0% | |
| GGGGAAAAGATGCCAATAAT+AGG | - | chr6.4:49712812-49712831 | MS.gene94035:CDS | 40.0% | |
| GTGATTCCCTTTGACATCAA+AGG | - | chr6.4:49713270-49713289 | MS.gene94035:intron | 40.0% | |
| TCCACAATGCAGACTTTACA+AGG | - | chr6.4:49714261-49714280 | MS.gene94035:CDS | 40.0% | |
| TCTTGCAGATAGCACTAAGA+TGG | - | chr6.4:49714119-49714138 | MS.gene94035:CDS | 40.0% | |
| TGGATCTCTACTTGATTCAC+TGG | - | chr6.4:49713196-49713215 | MS.gene94035:intron | 40.0% | |
| ! | AGGCTTTAACTTCACAGGTA+AGG | - | chr6.4:49713245-49713264 | MS.gene94035:intron | 40.0% |
| ! | CTAAAGCTTTTGACACAGCT+TGG | + | chr6.4:49712964-49712983 | None:intergenic | 40.0% |
| ! | GGTTGCATTTTCCAAGAGTA+CGG | + | chr6.4:49713379-49713398 | None:intergenic | 40.0% |
| ! | GTTGAAGGCTTTAACTTCAC+AGG | - | chr6.4:49713240-49713259 | MS.gene94035:intron | 40.0% |
| ! | TAGAAAGAAGAGTGTAGCTC+AGG | - | chr6.4:49713708-49713727 | MS.gene94035:CDS | 40.0% |
| ! | TTGGGGTTCTAATGCTGTTA+TGG | - | chr6.4:49713654-49713673 | MS.gene94035:intron | 40.0% |
| !! | GTGTCAAAAGCTTTAGAGCT+CGG | - | chr6.4:49712968-49712987 | MS.gene94035:CDS | 40.0% |
| !! | TTTTGAGAGCCTTGAGAACA+AGG | + | chr6.4:49713060-49713079 | None:intergenic | 40.0% |
| ACAAATCCTTGGAAGAGCTG+TGG | - | chr6.4:49714314-49714333 | MS.gene94035:CDS | 45.0% | |
| ATGAATCCATCTTGGCAGCA+AGG | - | chr6.4:49713556-49713575 | MS.gene94035:intron | 45.0% | |
| CAAATCCTTGGAAGAGCTGT+GGG | - | chr6.4:49714315-49714334 | MS.gene94035:CDS | 45.0% | |
| GAGAGAACAATGCTTGCTTG+AGG | + | chr6.4:49713087-49713106 | None:intergenic | 45.0% | |
| GCAGTTCCTAGGCCTATTAT+TGG | + | chr6.4:49712827-49712846 | None:intergenic | 45.0% | |
| GCCTTGTAAAGTCTGCATTG+TGG | + | chr6.4:49714265-49714284 | None:intergenic | 45.0% | |
| TCACCATGACCTTGTTCTCA+AGG | - | chr6.4:49713048-49713067 | MS.gene94035:intron | 45.0% | |
| TGCTAGATAAAGGGCCTGTT+TGG | - | chr6.4:49713815-49713834 | MS.gene94035:CDS | 45.0% | |
| TTGGCAGCAAGGAAAACTCA+GGG | - | chr6.4:49713567-49713586 | MS.gene94035:intron | 45.0% | |
| ! | AGTTTTCCTTGCTGCCAAGA+TGG | + | chr6.4:49713565-49713584 | None:intergenic | 45.0% |
| ! | GAATCTGAGGTTGGAGTATG+TGG | - | chr6.4:49713176-49713195 | MS.gene94035:intron | 45.0% |
| ! | TATGGTACCGAAGAACCTCT+TGG | - | chr6.4:49712938-49712957 | MS.gene94035:CDS | 45.0% |
| ! | TTGGTTGATGCCCTTGACAT+TGG | - | chr6.4:49712887-49712906 | MS.gene94035:CDS | 45.0% |
| AGCATCAGTGCACCATAGCT+TGG | + | chr6.4:49713029-49713048 | None:intergenic | 50.0% | |
| AGCTTGGCCAAGAGGTTCTT+CGG | + | chr6.4:49712948-49712967 | None:intergenic | 50.0% | |
| CTTGGCAGCAAGGAAAACTC+AGG | - | chr6.4:49713566-49713585 | MS.gene94035:intron | 50.0% | |
| GAACAATGCTTGCTTGAGGG+TGG | + | chr6.4:49713083-49713102 | None:intergenic | 50.0% | |
| GAGCCTTGAGAACAAGGTCA+TGG | + | chr6.4:49713054-49713073 | None:intergenic | 50.0% | |
| GAGGAGTTGTAGCAGTTCCT+AGG | + | chr6.4:49712838-49712857 | None:intergenic | 50.0% | |
| GGCAGATTACGAGAGCTCAT+TGG | - | chr6.4:49713878-49713897 | MS.gene94035:CDS | 50.0% | |
| GTGTGTGCATGGTCACCATT+GGG | - | chr6.4:49713616-49713635 | MS.gene94035:intron | 50.0% | |
| TGTGTGTGCATGGTCACCAT+TGG | - | chr6.4:49713615-49713634 | MS.gene94035:intron | 50.0% | |
| TTTGACACAGCTTGGCCAAG+AGG | + | chr6.4:49712956-49712975 | None:intergenic | 50.0% | |
| ACCATCCCACAGCTCTTCCA+AGG | + | chr6.4:49714323-49714342 | None:intergenic | 55.0% | |
| TCCTTGGAAGAGCTGTGGGA+TGG | - | chr6.4:49714319-49714338 | MS.gene94035:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 49712773 | 49714351 | 49712773 | ID=MS.gene94035 |
| chr6.4 | mRNA | 49712773 | 49714351 | 49712773 | ID=MS.gene94035.t1;Parent=MS.gene94035 |
| chr6.4 | exon | 49714026 | 49714351 | 49714026 | ID=MS.gene94035.t1.exon1;Parent=MS.gene94035.t1 |
| chr6.4 | CDS | 49714026 | 49714351 | 49714026 | ID=cds.MS.gene94035.t1;Parent=MS.gene94035.t1 |
| chr6.4 | exon | 49713705 | 49713948 | 49713705 | ID=MS.gene94035.t1.exon2;Parent=MS.gene94035.t1 |
| chr6.4 | CDS | 49713705 | 49713948 | 49713705 | ID=cds.MS.gene94035.t1;Parent=MS.gene94035.t1 |
| chr6.4 | exon | 49713395 | 49713574 | 49713395 | ID=MS.gene94035.t1.exon3;Parent=MS.gene94035.t1 |
| chr6.4 | CDS | 49713395 | 49713574 | 49713395 | ID=cds.MS.gene94035.t1;Parent=MS.gene94035.t1 |
| chr6.4 | exon | 49712773 | 49712997 | 49712773 | ID=MS.gene94035.t1.exon4;Parent=MS.gene94035.t1 |
| chr6.4 | CDS | 49712773 | 49712997 | 49712773 | ID=cds.MS.gene94035.t1;Parent=MS.gene94035.t1 |
| Gene Sequence |
| Protein sequence |
