Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047825.01 | MsG0180000739.01 | 0.800409 | 1.460901e-48 | 6.797542e-46 |
| MsG0080047868.01 | MsG0180000739.01 | 0.818487 | 1.910330e-52 | 1.431943e-49 |
| MsG0080047912.01 | MsG0180000739.01 | 0.890010 | 1.449050e-73 | 1.196938e-69 |
| MsG0080048656.01 | MsG0180000739.01 | 0.823605 | 1.265763e-53 | 1.095032e-50 |
| MsG0080048843.01 | MsG0180000739.01 | 0.836545 | 8.789818e-57 | 1.111201e-53 |
| MsG0080048968.01 | MsG0180000739.01 | 0.802942 | 4.416168e-49 | 2.191128e-46 |
| MsG0180000157.01 | MsG0180000739.01 | 0.819721 | 1.001146e-52 | 7.765255e-50 |
| MsG0180000669.01 | MsG0180000739.01 | 0.810097 | 1.365298e-50 | 8.156595e-48 |
| MsG0180000739.01 | MsG0180001043.01 | 0.829793 | 4.219941e-55 | 4.364637e-52 |
| MsG0180000739.01 | MsG0180002075.01 | 0.815233 | 1.026532e-51 | 7.037447e-49 |
| MsG0180000739.01 | MsG0180002489.01 | 0.803681 | 3.103704e-49 | 1.569425e-46 |
| MsG0180000739.01 | MsG0180003444.01 | 0.803250 | 3.812390e-49 | 1.906589e-46 |
| MsG0180000739.01 | MsG0180005125.01 | 0.826814 | 2.206612e-54 | 2.091785e-51 |
| MsG0180000739.01 | MsG0180005350.01 | 0.800529 | 1.380960e-48 | 6.444877e-46 |
| MsG0180000739.01 | MsG0180006016.01 | 0.809098 | 2.238083e-50 | 1.302207e-47 |
| MsG0180000739.01 | MsG0180006023.01 | 0.805622 | 1.221219e-49 | 6.491608e-47 |
| MsG0180000739.01 | MsG0280006728.01 | 0.803007 | 4.280888e-49 | 2.127462e-46 |
| MsG0180000739.01 | MsG0280007609.01 | 0.816112 | 6.539857e-52 | 4.593020e-49 |
| MsG0180000739.01 | MsG0280008239.01 | 0.817776 | 2.767352e-52 | 2.034119e-49 |
| MsG0180000739.01 | MsG0280008971.01 | 0.828749 | 7.560569e-55 | 7.582558e-52 |
| MsG0180000739.01 | MsG0280010389.01 | 0.803911 | 2.781368e-49 | 1.414878e-46 |
| MsG0180000739.01 | MsG0280010551.01 | 0.810907 | 9.127126e-51 | 5.571288e-48 |
| MsG0180000739.01 | MsG0280011070.01 | 0.808712 | 2.706113e-50 | 1.558327e-47 |
| MsG0180000739.01 | MsG0380011754.01 | 0.811183 | 7.954106e-51 | 4.890592e-48 |
| MsG0180000739.01 | MsG0380012186.01 | 0.807637 | 4.585678e-50 | 2.567372e-47 |
| MsG0180000739.01 | MsG0380015779.01 | 0.849465 | 3.177417e-60 | 6.028482e-57 |
| MsG0180000739.01 | MsG0380016227.01 | 0.813717 | 2.222209e-51 | 1.462271e-48 |
| MsG0180000739.01 | MsG0380016752.01 | 0.821827 | 3.282632e-53 | 2.700164e-50 |
| MsG0180000739.01 | MsG0380017226.01 | 0.811225 | 7.788928e-51 | 4.794322e-48 |
| MsG0180000739.01 | MsG0380017403.01 | 0.810396 | 1.176776e-50 | 7.085303e-48 |
| MsG0180000739.01 | MsG0380017604.01 | 0.823614 | 1.259694e-53 | 1.090072e-50 |
| MsG0180000739.01 | MsG0380017631.01 | 0.826909 | 2.094095e-54 | 1.990724e-51 |
| MsG0180000739.01 | MsG0380018009.01 | 0.806250 | 9.012837e-50 | 4.868676e-47 |
| MsG0180000739.01 | MsG0480020719.01 | 0.807725 | 4.393261e-50 | 2.465331e-47 |
| MsG0180000739.01 | MsG0480020720.01 | 0.841980 | 3.417899e-58 | 5.108143e-55 |
| MsG0180000739.01 | MsG0480021731.01 | 0.812977 | 3.230493e-51 | 2.083378e-48 |
| MsG0180000739.01 | MsG0480022768.01 | 0.803310 | 3.706260e-49 | 1.856286e-46 |
| MsG0180000739.01 | MsG0480023517.01 | 0.800959 | 1.128436e-48 | 5.324450e-46 |
| MsG0180000739.01 | MsG0580024114.01 | 0.804697 | 1.907096e-49 | 9.899262e-47 |
| MsG0180000739.01 | MsG0580024581.01 | 0.802447 | 5.586617e-49 | 2.737245e-46 |
| MsG0180000739.01 | MsG0580024733.01 | 0.808266 | 3.369468e-50 | 1.918009e-47 |
| MsG0180000739.01 | MsG0580025134.01 | 0.810427 | 1.158857e-50 | 6.983256e-48 |
| MsG0180000739.01 | MsG0580025235.01 | 0.815266 | 1.009473e-51 | 6.927029e-49 |
| MsG0180000739.01 | MsG0580025453.01 | 0.803326 | 3.676437e-49 | 1.842138e-46 |
| MsG0180000739.01 | MsG0580025769.01 | 0.808544 | 2.939939e-50 | 1.685647e-47 |
| MsG0180000739.01 | MsG0580025799.01 | 0.807368 | 5.230641e-50 | 2.908021e-47 |
| MsG0180000739.01 | MsG0580027034.01 | 0.810570 | 1.079641e-50 | 6.530637e-48 |
| MsG0180000739.01 | MsG0580028900.01 | 0.800083 | 1.701762e-48 | 7.852931e-46 |
| MsG0180000739.01 | MsG0580029258.01 | 0.820580 | 6.364703e-53 | 5.055522e-50 |
| MsG0180000739.01 | MsG0580029594.01 | 0.820004 | 8.623968e-53 | 6.742304e-50 |
| MsG0180000739.01 | MsG0580029629.01 | 0.803323 | 3.681846e-49 | 1.844719e-46 |
| MsG0180000739.01 | MsG0580030235.01 | 0.833279 | 5.842908e-56 | 6.696703e-53 |
| MsG0180000739.01 | MsG0680030685.01 | 0.800393 | 1.472167e-48 | 6.847030e-46 |
| MsG0180000739.01 | MsG0680031538.01 | 0.806484 | 8.043811e-50 | 4.371104e-47 |
| MsG0180000739.01 | MsG0680033391.01 | 0.810070 | 1.383930e-50 | 8.262065e-48 |
| MsG0180000739.01 | MsG0780036609.01 | 0.809403 | 1.925335e-50 | 1.129393e-47 |
| MsG0180000739.01 | MsG0780036696.01 | 0.824282 | 8.781747e-54 | 7.743316e-51 |
| MsG0180000739.01 | MsG0780037512.01 | 0.805675 | 1.190742e-49 | 6.338096e-47 |
| MsG0180000739.01 | MsG0780039943.01 | 0.808646 | 2.795758e-50 | 1.607188e-47 |
| MsG0180000739.01 | MsG0780040182.01 | 0.806124 | 9.577957e-50 | 5.157324e-47 |
| MsG0180000739.01 | MsG0780040726.01 | 0.806689 | 7.282326e-50 | 3.977985e-47 |
| MsG0180000739.01 | MsG0780040861.01 | 0.830000 | 3.757201e-55 | 3.908693e-52 |
| MsG0180000739.01 | MsG0780041222.01 | 0.808408 | 3.142936e-50 | 1.795681e-47 |
| MsG0180000739.01 | MsG0780041399.01 | 0.815512 | 8.898307e-52 | 6.147752e-49 |
| MsG0180000739.01 | MsG0880042053.01 | 0.810985 | 8.776635e-51 | 5.369042e-48 |
| MsG0180000739.01 | MsG0880045583.01 | 0.816776 | 4.645596e-52 | 3.322376e-49 |
| MsG0180000739.01 | MsG0880046058.01 | 0.810098 | 1.364912e-50 | 8.154431e-48 |
| MsG0180000739.01 | MsG0880046091.01 | 0.841698 | 4.057471e-58 | 6.009818e-55 |
| MsG0180000739.01 | MsG0880046244.01 | 0.820831 | 5.572001e-53 | 4.457336e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000739.01.T01 | MTR_8g064930 | 60.000 | 40 | 16 | 0 | 1 | 40 | 11 | 50 | 8.70e-13 | 57.4 |
| MsG0180000739.01.T01 | MTR_0091s0080 | 50.000 | 48 | 24 | 0 | 1 | 48 | 259 | 306 | 1.16e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGAATGACATTGATATTC+TGG | 0.189504 | 1:-10485894 | None:intergenic |
| CCAGAATATCAATGTCATTC+AGG | 0.310628 | 1:+10485894 | MsG0180000739.01.T01:CDS |
| TCCTGTTTGTTATAGCCATA+AGG | 0.397282 | 1:-10486030 | None:intergenic |
| ACAAAATAGAGCTGGGTGCC+TGG | 0.399485 | 1:+10485975 | MsG0180000739.01.T01:CDS |
| ATGACATTGATATTCTGGTT+TGG | 0.423705 | 1:-10485889 | None:intergenic |
| TGGCTATAACAAACAGGAAA+GGG | 0.435466 | 1:+10486035 | MsG0180000739.01.T01:CDS |
| GTATCCGAATCTACTCCTTA+TGG | 0.451663 | 1:+10486015 | MsG0180000739.01.T01:CDS |
| CGCATATGTCAGGCAAATCC+AGG | 0.467174 | 1:-10485993 | None:intergenic |
| TCCTTATGGCTATAACAAAC+AGG | 0.471089 | 1:+10486029 | MsG0180000739.01.T01:CDS |
| AGAGTTCTACAAAATAGAGC+TGG | 0.478218 | 1:+10485967 | MsG0180000739.01.T01:CDS |
| ACATTCTTTGACAAAACAGT+TGG | 0.514565 | 1:-10485830 | None:intergenic |
| GATTCGGATACGCATATGTC+AGG | 0.519976 | 1:-10486003 | None:intergenic |
| ATAGCCATAAGGAGTAGATT+CGG | 0.520589 | 1:-10486019 | None:intergenic |
| ATGGCTATAACAAACAGGAA+AGG | 0.562348 | 1:+10486034 | MsG0180000739.01.T01:CDS |
| GAGTTCTACAAAATAGAGCT+GGG | 0.605880 | 1:+10485968 | MsG0180000739.01.T01:CDS |
| GGCTATAACAAACAGGAAAG+GGG | 0.627042 | 1:+10486036 | MsG0180000739.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATTCTTTGACAAAACAGT+TGG | - | Chr1:10485833-10485852 | None:intergenic | 30.0% | |
| ! | AAAACGAGAAGGTCTATTTT+CGG | + | Chr1:10485926-10485945 | MsG0180000739.01.T01:CDS | 30.0% |
| ! | AACTGTTTTGTCAAAGAATG+TGG | + | Chr1:10485832-10485851 | MsG0180000739.01.T01:CDS | 30.0% |
| ! | ATGACATTGATATTCTGGTT+TGG | - | Chr1:10485892-10485911 | None:intergenic | 30.0% |
| AGAGTTCTACAAAATAGAGC+TGG | + | Chr1:10485967-10485986 | MsG0180000739.01.T01:CDS | 35.0% | |
| ATAGCCATAAGGAGTAGATT+CGG | - | Chr1:10486022-10486041 | None:intergenic | 35.0% | |
| ATGGCTATAACAAACAGGAA+AGG | + | Chr1:10486034-10486053 | MsG0180000739.01.T01:CDS | 35.0% | |
| CCAGAATATCAATGTCATTC+AGG | + | Chr1:10485894-10485913 | MsG0180000739.01.T01:CDS | 35.0% | |
| CCTGAATGACATTGATATTC+TGG | - | Chr1:10485897-10485916 | None:intergenic | 35.0% | |
| GAGTTCTACAAAATAGAGCT+GGG | + | Chr1:10485968-10485987 | MsG0180000739.01.T01:CDS | 35.0% | |
| TCCTTATGGCTATAACAAAC+AGG | + | Chr1:10486029-10486048 | MsG0180000739.01.T01:CDS | 35.0% | |
| TGGCTATAACAAACAGGAAA+GGG | + | Chr1:10486035-10486054 | MsG0180000739.01.T01:CDS | 35.0% | |
| !! | GGTTGATAAGAAAAACGAGA+AGG | + | Chr1:10485915-10485934 | MsG0180000739.01.T01:CDS | 35.0% |
| !! | TCCTGTTTGTTATAGCCATA+AGG | - | Chr1:10486033-10486052 | None:intergenic | 35.0% |
| GGCTATAACAAACAGGAAAG+GGG | + | Chr1:10486036-10486055 | MsG0180000739.01.T01:CDS | 40.0% | |
| GTATCCGAATCTACTCCTTA+TGG | + | Chr1:10486015-10486034 | MsG0180000739.01.T01:CDS | 40.0% | |
| GATTCGGATACGCATATGTC+AGG | - | Chr1:10486006-10486025 | None:intergenic | 45.0% | |
| ! | GAGAAGGTCTATTTTCGGCA+GGG | + | Chr1:10485931-10485950 | MsG0180000739.01.T01:CDS | 45.0% |
| ACAAAATAGAGCTGGGTGCC+TGG | + | Chr1:10485975-10485994 | MsG0180000739.01.T01:CDS | 50.0% | |
| CGCATATGTCAGGCAAATCC+AGG | - | Chr1:10485996-10486015 | None:intergenic | 50.0% | |
| ! | AGGTCTATTTTCGGCAGGGT+TGG | + | Chr1:10485935-10485954 | MsG0180000739.01.T01:CDS | 50.0% |
| ! | CGAGAAGGTCTATTTTCGGC+AGG | + | Chr1:10485930-10485949 | MsG0180000739.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 10485826 | 10486062 | 10485826 | ID=MsG0180000739.01;Name=MsG0180000739.01 |
| Chr1 | mRNA | 10485826 | 10486062 | 10485826 | ID=MsG0180000739.01.T01;Parent=MsG0180000739.01;Name=MsG0180000739.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|78 |
| Chr1 | exon | 10485826 | 10486062 | 10485826 | ID=MsG0180000739.01.T01:exon:1284;Parent=MsG0180000739.01.T01 |
| Chr1 | CDS | 10485826 | 10486062 | 10485826 | ID=MsG0180000739.01.T01:cds;Parent=MsG0180000739.01.T01 |
| Gene Sequence |
| Protein sequence |
