Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012186.01.T01 | XP_013458995.1 | 99.383 | 162 | 1 | 0 | 1 | 162 | 209 | 370 | 4.93E-109 | 325 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012186.01.T01 | Q8L7A4 | 83.951 | 162 | 26 | 0 | 1 | 162 | 224 | 385 | 8.08E-95 | 282 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012186.01.T01 | A0A072UVE7 | 99.383 | 162 | 1 | 0 | 1 | 162 | 209 | 370 | 2.36e-109 | 325 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047825.01 | MsG0380012186.01 | 0.816602 | 5.080432e-52 | 3.616131e-49 |
| MsG0080048843.01 | MsG0380012186.01 | 0.815964 | 7.058880e-52 | 4.937348e-49 |
| MsG0080048968.01 | MsG0380012186.01 | 0.833415 | 5.402474e-56 | 6.217540e-53 |
| MsG0180000055.01 | MsG0380012186.01 | 0.802435 | 5.618267e-49 | 2.751935e-46 |
| MsG0180000157.01 | MsG0380012186.01 | 0.820994 | 5.110666e-53 | 4.107220e-50 |
| MsG0180000611.01 | MsG0380012186.01 | 0.807383 | 5.191314e-50 | 2.887403e-47 |
| MsG0180000669.01 | MsG0380012186.01 | 0.804892 | 1.737138e-49 | 9.063258e-47 |
| MsG0180000739.01 | MsG0380012186.01 | 0.807637 | 4.585678e-50 | 2.567372e-47 |
| MsG0180000822.01 | MsG0380012186.01 | 0.824344 | 8.491687e-54 | 7.500883e-51 |
| MsG0180001043.01 | MsG0380012186.01 | 0.847658 | 1.006351e-59 | 1.801444e-56 |
| MsG0180001355.01 | MsG0380012186.01 | 0.827153 | 1.831079e-54 | 1.753045e-51 |
| MsG0180001712.01 | MsG0380012186.01 | 0.803689 | 3.091609e-49 | 1.563670e-46 |
| MsG0180001921.01 | MsG0380012186.01 | 0.824993 | 5.973868e-54 | 5.376829e-51 |
| MsG0180002075.01 | MsG0380012186.01 | 0.836842 | 7.381157e-57 | 9.415549e-54 |
| MsG0180004388.01 | MsG0380012186.01 | 0.809209 | 2.119217e-50 | 1.236703e-47 |
| MsG0180004767.01 | MsG0380012186.01 | 0.804813 | 1.803657e-49 | 9.390864e-47 |
| MsG0180004824.01 | MsG0380012186.01 | 0.834556 | 2.800207e-56 | 3.335041e-53 |
| MsG0180005125.01 | MsG0380012186.01 | 0.822628 | 2.139798e-53 | 1.800907e-50 |
| MsG0180005233.01 | MsG0380012186.01 | 0.845192 | 4.734177e-59 | 7.827040e-56 |
| MsG0180005268.01 | MsG0380012186.01 | 0.803583 | 3.253475e-49 | 1.641142e-46 |
| MsG0180005306.01 | MsG0380012186.01 | 0.832560 | 8.818137e-56 | 9.892348e-53 |
| MsG0180005350.01 | MsG0380012186.01 | 0.801543 | 8.570197e-49 | 4.103618e-46 |
| MsG0180005944.01 | MsG0380012186.01 | 0.810692 | 1.016060e-50 | 6.166378e-48 |
| MsG0180006016.01 | MsG0380012186.01 | 0.812489 | 4.131604e-51 | 2.630609e-48 |
| MsG0280011043.01 | MsG0380012186.01 | 0.811693 | 6.161931e-51 | 3.839826e-48 |
| MsG0280011235.01 | MsG0380012186.01 | 0.809180 | 2.149482e-50 | 1.253391e-47 |
| MsG0380011754.01 | MsG0380012186.01 | 0.821130 | 4.754860e-53 | 3.835484e-50 |
| MsG0380012185.01 | MsG0380012186.01 | 0.827967 | 1.167617e-54 | 1.144775e-51 |
| MsG0380012186.01 | MsG0380012187.01 | 0.846701 | 1.840597e-59 | 3.195437e-56 |
| MsG0380012186.01 | MsG0380014908.01 | 0.821609 | 3.687096e-53 | 3.013923e-50 |
| MsG0380012186.01 | MsG0380015779.01 | 0.844900 | 5.677800e-59 | 9.300283e-56 |
| MsG0380012186.01 | MsG0380015818.01 | 0.810728 | 9.977434e-51 | 6.061197e-48 |
| MsG0380012186.01 | MsG0380015893.01 | 0.808735 | 2.676130e-50 | 1.541958e-47 |
| MsG0380012186.01 | MsG0380015934.01 | 0.817853 | 2.657817e-52 | 1.957769e-49 |
| MsG0380012186.01 | MsG0380016227.01 | 0.831491 | 1.620298e-55 | 1.761059e-52 |
| MsG0380012186.01 | MsG0380017226.01 | 0.801549 | 8.543815e-49 | 4.091595e-46 |
| MsG0380012186.01 | MsG0380017604.01 | 0.832768 | 7.829396e-56 | 8.837527e-53 |
| MsG0380012186.01 | MsG0380017631.01 | 0.827904 | 1.209080e-54 | 1.183160e-51 |
| MsG0380012186.01 | MsG0480018640.01 | 0.800393 | 1.472067e-48 | 6.846589e-46 |
| MsG0380012186.01 | MsG0480018970.01 | 0.809345 | 1.981053e-50 | 1.160301e-47 |
| MsG0380012186.01 | MsG0480020720.01 | 0.826236 | 3.030569e-54 | 2.825833e-51 |
| MsG0380012186.01 | MsG0480020843.01 | 0.819666 | 1.030172e-52 | 7.978108e-50 |
| MsG0380012186.01 | MsG0480022035.01 | 0.829789 | 4.230004e-55 | 4.374492e-52 |
| MsG0380012186.01 | MsG0480023517.01 | 0.825210 | 5.306600e-54 | 4.805887e-51 |
| MsG0380012186.01 | MsG0480023554.01 | 0.809865 | 1.531700e-50 | 9.095409e-48 |
| MsG0380012186.01 | MsG0480023776.01 | 0.801252 | 9.828085e-49 | 4.671640e-46 |
| MsG0380012186.01 | MsG0580024114.01 | 0.810907 | 9.126611e-51 | 5.570979e-48 |
| MsG0380012186.01 | MsG0580025235.01 | 0.803949 | 2.730426e-49 | 1.390338e-46 |
| MsG0380012186.01 | MsG0580025278.01 | 0.826090 | 3.282869e-54 | 3.048266e-51 |
| MsG0380012186.01 | MsG0580025328.01 | 0.814613 | 1.408919e-51 | 9.498680e-49 |
| MsG0380012186.01 | MsG0580025453.01 | 0.809705 | 1.658107e-50 | 9.804717e-48 |
| MsG0380012186.01 | MsG0580025799.01 | 0.827931 | 1.191173e-54 | 1.166589e-51 |
| MsG0380012186.01 | MsG0580027034.01 | 0.831121 | 1.996973e-55 | 2.146738e-52 |
| MsG0380012186.01 | MsG0580027441.01 | 0.830741 | 2.476272e-55 | 2.632313e-52 |
| MsG0380012186.01 | MsG0580029258.01 | 0.822546 | 2.235702e-53 | 1.877172e-50 |
| MsG0380012186.01 | MsG0580029594.01 | 0.802572 | 5.263145e-49 | 2.586914e-46 |
| MsG0380012186.01 | MsG0580029629.01 | 0.806370 | 8.499907e-50 | 4.605551e-47 |
| MsG0380012186.01 | MsG0580029758.01 | 0.822400 | 2.417905e-53 | 2.021728e-50 |
| MsG0380012186.01 | MsG0580030204.01 | 0.819239 | 1.289120e-52 | 9.865462e-50 |
| MsG0380012186.01 | MsG0580030235.01 | 0.825523 | 4.475332e-54 | 4.088951e-51 |
| MsG0380012186.01 | MsG0680030685.01 | 0.813483 | 2.501665e-51 | 1.635773e-48 |
| MsG0380012186.01 | MsG0680031459.01 | 0.820569 | 6.399575e-53 | 5.081886e-50 |
| MsG0380012186.01 | MsG0680031538.01 | 0.843078 | 1.747896e-58 | 2.703523e-55 |
| MsG0380012186.01 | MsG0680031651.01 | 0.800274 | 1.556097e-48 | 7.215594e-46 |
| MsG0380012186.01 | MsG0680034476.01 | 0.807917 | 3.997946e-50 | 2.254699e-47 |
| MsG0380012186.01 | MsG0680035582.01 | 0.804797 | 1.817920e-49 | 9.460903e-47 |
| MsG0380012186.01 | MsG0680035854.01 | 0.823613 | 1.260438e-53 | 1.090691e-50 |
| MsG0380012186.01 | MsG0780036240.01 | 0.818786 | 1.634665e-52 | 1.235406e-49 |
| MsG0380012186.01 | MsG0780036635.01 | 0.804001 | 2.663138e-49 | 1.357843e-46 |
| MsG0380012186.01 | MsG0780036696.01 | 0.817119 | 3.889796e-52 | 2.808037e-49 |
| MsG0380012186.01 | MsG0780039340.01 | 0.802275 | 6.060298e-49 | 2.956381e-46 |
| MsG0380012186.01 | MsG0780039875.01 | 0.823781 | 1.151561e-53 | 1.001305e-50 |
| MsG0380012186.01 | MsG0780039943.01 | 0.819815 | 9.526898e-53 | 7.409054e-50 |
| MsG0380012186.01 | MsG0780040726.01 | 0.817743 | 2.814493e-52 | 2.066939e-49 |
| MsG0380012186.01 | MsG0780040861.01 | 0.826613 | 2.464764e-54 | 2.322974e-51 |
| MsG0380012186.01 | MsG0780041399.01 | 0.817829 | 2.691095e-52 | 1.980974e-49 |
| MsG0380012186.01 | MsG0780041441.01 | 0.801180 | 1.016668e-48 | 4.823808e-46 |
| MsG0380012186.01 | MsG0880042053.01 | 0.856603 | 2.877442e-62 | 6.905799e-59 |
| MsG0380012186.01 | MsG0880042107.01 | 0.802907 | 4.488790e-49 | 2.225316e-46 |
| MsG0380012186.01 | MsG0880042611.01 | 0.814179 | 1.757921e-51 | 1.171286e-48 |
| MsG0380012186.01 | MsG0880043002.01 | 0.814487 | 1.502663e-51 | 1.009640e-48 |
| MsG0380012186.01 | MsG0880043835.01 | 0.802780 | 4.768789e-49 | 2.356545e-46 |
| MsG0380012186.01 | MsG0880046058.01 | 0.807369 | 5.227514e-50 | 2.906389e-47 |
| MsG0380012186.01 | MsG0880046091.01 | 0.835477 | 1.640743e-56 | 2.008659e-53 |
| MsG0380012186.01 | MsG0880046731.01 | 0.804352 | 2.250918e-49 | 1.158037e-46 |
| MsG0380012186.01 | MsG0880047143.01 | 0.803402 | 3.546266e-49 | 1.780469e-46 |
| MsG0280008239.01 | MsG0380012186.01 | 0.821196 | 4.590239e-53 | 3.709719e-50 |
| MsG0280008295.01 | MsG0380012186.01 | 0.808893 | 2.475410e-50 | 1.432307e-47 |
| MsG0280008971.01 | MsG0380012186.01 | 0.805908 | 1.063508e-49 | 5.694990e-47 |
| MsG0280009235.01 | MsG0380012186.01 | 0.828504 | 8.668257e-55 | 8.632531e-52 |
| MsG0280010423.01 | MsG0380012186.01 | -0.807208 | 5.654435e-50 | 3.130711e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012186.01.T01 | MTR_3g017500 | 99.383 | 162 | 1 | 0 | 1 | 162 | 209 | 370 | 5.97e-113 | 325 |
| MsG0380012186.01.T01 | MTR_3g017500 | 99.383 | 162 | 1 | 0 | 1 | 162 | 239 | 400 | 1.60e-112 | 325 |
| MsG0380012186.01.T01 | MTR_8g038400 | 83.951 | 162 | 26 | 0 | 1 | 162 | 206 | 367 | 2.61e-95 | 280 |
| MsG0380012186.01.T01 | MTR_8g088320 | 54.938 | 162 | 73 | 0 | 1 | 162 | 161 | 322 | 3.30e-63 | 197 |
| MsG0380012186.01.T01 | MTR_2g030230 | 52.469 | 162 | 77 | 0 | 1 | 162 | 168 | 329 | 4.25e-60 | 189 |
| MsG0380012186.01.T01 | MTR_2g011590 | 44.720 | 161 | 80 | 2 | 2 | 158 | 1 | 156 | 5.91e-41 | 135 |
| MsG0380012186.01.T01 | MTR_3g067585 | 44.444 | 162 | 81 | 2 | 1 | 158 | 1 | 157 | 6.79e-41 | 135 |
| MsG0380012186.01.T01 | MTR_8g107340 | 41.250 | 160 | 93 | 1 | 1 | 160 | 17 | 175 | 3.03e-40 | 134 |
| MsG0380012186.01.T01 | MTR_2g027100 | 45.860 | 157 | 84 | 1 | 1 | 156 | 1 | 157 | 1.48e-37 | 127 |
| MsG0380012186.01.T01 | MTR_1g031650 | 44.444 | 153 | 83 | 2 | 5 | 156 | 6 | 157 | 2.77e-37 | 126 |
| MsG0380012186.01.T01 | MTR_6g069470 | 39.375 | 160 | 96 | 1 | 1 | 160 | 13 | 171 | 3.84e-34 | 118 |
| MsG0380012186.01.T01 | MTR_4g084280 | 39.394 | 165 | 91 | 2 | 1 | 161 | 1 | 160 | 9.77e-34 | 117 |
| MsG0380012186.01.T01 | MTR_1g031650 | 42.500 | 120 | 68 | 1 | 37 | 156 | 2 | 120 | 2.42e-24 | 92.0 |
| MsG0380012186.01.T01 | MTR_1g008950 | 36.264 | 91 | 56 | 2 | 6 | 94 | 4 | 94 | 3.01e-13 | 63.2 |
| MsG0380012186.01.T01 | MTR_8g032320 | 36.145 | 83 | 51 | 2 | 6 | 86 | 4 | 86 | 7.25e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012186.01.T01 | AT3G07940 | 83.951 | 162 | 26 | 0 | 1 | 162 | 224 | 385 | 8.24e-96 | 282 |
| MsG0380012186.01.T01 | AT4G21160 | 56.173 | 162 | 71 | 0 | 1 | 162 | 176 | 337 | 9.34e-66 | 204 |
| MsG0380012186.01.T01 | AT4G21160 | 56.173 | 162 | 71 | 0 | 1 | 162 | 176 | 337 | 9.34e-66 | 204 |
| MsG0380012186.01.T01 | AT4G21160 | 56.173 | 162 | 71 | 0 | 1 | 162 | 176 | 337 | 9.34e-66 | 204 |
| MsG0380012186.01.T01 | AT4G21160 | 56.173 | 162 | 71 | 0 | 1 | 162 | 176 | 337 | 9.34e-66 | 204 |
| MsG0380012186.01.T01 | AT4G05330 | 56.790 | 162 | 70 | 0 | 1 | 162 | 140 | 301 | 1.04e-65 | 202 |
| MsG0380012186.01.T01 | AT4G05330 | 56.790 | 162 | 70 | 0 | 1 | 162 | 175 | 336 | 3.52e-65 | 202 |
| MsG0380012186.01.T01 | AT4G05330 | 56.790 | 162 | 70 | 0 | 1 | 162 | 175 | 336 | 3.52e-65 | 202 |
| MsG0380012186.01.T01 | AT1G70790 | 44.444 | 153 | 84 | 1 | 5 | 156 | 6 | 158 | 2.75e-40 | 134 |
| MsG0380012186.01.T01 | AT1G70790 | 44.444 | 153 | 84 | 1 | 5 | 156 | 6 | 158 | 2.75e-40 | 134 |
| MsG0380012186.01.T01 | AT1G70790 | 44.444 | 153 | 84 | 1 | 5 | 156 | 6 | 158 | 2.75e-40 | 134 |
| MsG0380012186.01.T01 | AT1G66360 | 42.593 | 162 | 92 | 1 | 1 | 161 | 1 | 162 | 4.43e-40 | 133 |
| MsG0380012186.01.T01 | AT1G73580 | 42.500 | 160 | 91 | 1 | 1 | 160 | 4 | 162 | 1.29e-39 | 132 |
| MsG0380012186.01.T01 | AT1G70800 | 43.590 | 156 | 87 | 1 | 1 | 156 | 10 | 164 | 1.30e-39 | 132 |
| MsG0380012186.01.T01 | AT1G70810 | 41.667 | 156 | 90 | 1 | 1 | 156 | 1 | 155 | 2.42e-37 | 126 |
| MsG0380012186.01.T01 | AT5G37740 | 41.875 | 160 | 91 | 1 | 1 | 158 | 1 | 160 | 6.23e-37 | 125 |
| MsG0380012186.01.T01 | AT2G01540 | 42.484 | 153 | 87 | 1 | 5 | 156 | 6 | 158 | 4.86e-36 | 123 |
| MsG0380012186.01.T01 | AT5G47710 | 40.881 | 159 | 93 | 1 | 1 | 158 | 1 | 159 | 4.29e-35 | 120 |
| MsG0380012186.01.T01 | AT5G47710 | 40.881 | 159 | 93 | 1 | 1 | 158 | 1 | 159 | 4.29e-35 | 120 |
| MsG0380012186.01.T01 | AT5G47710 | 40.881 | 159 | 93 | 1 | 1 | 158 | 1 | 159 | 4.29e-35 | 120 |
| MsG0380012186.01.T01 | AT5G47710 | 40.881 | 159 | 93 | 1 | 1 | 158 | 1 | 159 | 4.29e-35 | 120 |
| MsG0380012186.01.T01 | AT1G48590 | 39.024 | 164 | 91 | 2 | 1 | 160 | 6 | 164 | 4.42e-35 | 120 |
| MsG0380012186.01.T01 | AT1G48590 | 39.024 | 164 | 91 | 2 | 1 | 160 | 6 | 164 | 4.42e-35 | 120 |
| MsG0380012186.01.T01 | AT1G48590 | 39.024 | 164 | 91 | 2 | 1 | 160 | 37 | 195 | 1.00e-34 | 120 |
| MsG0380012186.01.T01 | AT1G23140 | 37.888 | 161 | 99 | 1 | 1 | 161 | 1 | 160 | 2.39e-34 | 118 |
| MsG0380012186.01.T01 | AT5G37740 | 39.412 | 170 | 91 | 2 | 1 | 158 | 1 | 170 | 1.03e-33 | 117 |
| MsG0380012186.01.T01 | AT3G17980 | 36.585 | 164 | 95 | 2 | 1 | 160 | 13 | 171 | 1.03e-33 | 117 |
| MsG0380012186.01.T01 | AT1G70790 | 42.017 | 119 | 68 | 1 | 39 | 156 | 3 | 121 | 2.15e-24 | 92.8 |
| MsG0380012186.01.T01 | AT1G48590 | 30.488 | 164 | 73 | 3 | 1 | 160 | 37 | 163 | 3.06e-19 | 80.1 |
| MsG0380012186.01.T01 | AT3G07940 | 86.486 | 37 | 5 | 0 | 1 | 37 | 224 | 260 | 5.42e-15 | 70.9 |
| MsG0380012186.01.T01 | AT1G03370 | 37.778 | 90 | 55 | 1 | 8 | 96 | 3 | 92 | 5.45e-13 | 66.2 |
| MsG0380012186.01.T01 | AT1G03370 | 37.778 | 90 | 55 | 1 | 8 | 96 | 3 | 92 | 7.93e-13 | 65.5 |
Find 28 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAGGTTAATGTGGTTAA+AGG | 0.230072 | 3:-12385983 | MsG0380012186.01.T01:CDS |
| TGAATTTGTTGGATTGATTA+AGG | 0.287189 | 3:-12385999 | MsG0380012186.01.T01:CDS |
| AGTCAATGCAGCTTGGAAAA+TGG | 0.292719 | 3:-12385228 | MsG0380012186.01.T01:CDS |
| AGAACAATTTAAATCCTGTT+TGG | 0.340329 | 3:-12385771 | MsG0380012186.01.T01:CDS |
| CATTATTGATGATTTCTCAT+AGG | 0.350483 | 3:+12385252 | None:intergenic |
| GCAGGTATGGTTGAATTTGT+TGG | 0.378256 | 3:-12386010 | None:intergenic |
| CCTTATGTCATCCTTTCTCT+TGG | 0.379448 | 3:-12385923 | MsG0380012186.01.T01:CDS |
| GTCAATGCAGCTTGGAAAAT+GGG | 0.386273 | 3:-12385227 | MsG0380012186.01.T01:CDS |
| TATCTCATCTATTGAGTAAG+AGG | 0.392319 | 3:+12385073 | None:intergenic |
| TTAAGAGGAGGGATGTTCTC+AGG | 0.396970 | 3:+12385724 | None:intergenic |
| CATCCTTTCTCTTGGTCACC+AGG | 0.414383 | 3:-12385915 | MsG0380012186.01.T01:intron |
| GGAATAATTTCTCTTGAAGA+AGG | 0.433305 | 3:-12385172 | MsG0380012186.01.T01:CDS |
| TCTCAGGGATTGAAAGCATT+AGG | 0.434077 | 3:+12385740 | None:intergenic |
| ATTAGGCTTTCATTCCAAAC+AGG | 0.437169 | 3:+12385757 | None:intergenic |
| CTATGAGAAATCATCAATAA+TGG | 0.437790 | 3:-12385251 | MsG0380012186.01.T01:CDS |
| TGAAGAAGGGAAAGTGAGAC+AGG | 0.489048 | 3:-12385158 | MsG0380012186.01.T01:CDS |
| CCAAGAGAAAGGATGACATA+AGG | 0.500819 | 3:+12385923 | None:intergenic |
| TCACTATATCTCTGATAGCT+AGG | 0.520516 | 3:+12385954 | None:intergenic |
| CAAGAGAAAGGATGACATAA+GGG | 0.522273 | 3:+12385924 | None:intergenic |
| GACAATACACTTGTGAAAGA+TGG | 0.535420 | 3:-12385193 | MsG0380012186.01.T01:CDS |
| TAAGAGGAGGGATGTTCTCA+GGG | 0.542262 | 3:+12385725 | None:intergenic |
| TCACCTGGTGACCAAGAGAA+AGG | 0.560168 | 3:+12385912 | None:intergenic |
| GAATAATTTCTCTTGAAGAA+GGG | 0.562106 | 3:-12385171 | MsG0380012186.01.T01:CDS |
| ATAATGGAGTCAATGCAGCT+TGG | 0.580263 | 3:-12385235 | MsG0380012186.01.T01:CDS |
| TGGATTGATTAAGGTTAATG+TGG | 0.585859 | 3:-12385990 | MsG0380012186.01.T01:CDS |
| CTTGGAAAATGGGTAGCAAG+TGG | 0.649585 | 3:-12385217 | MsG0380012186.01.T01:CDS |
| AGGCTACAAAATGTTGAGAG+AGG | 0.650552 | 3:-12385124 | MsG0380012186.01.T01:CDS |
| TGAGACAGGAAATCTCATTG+AGG | 0.699747 | 3:-12385144 | MsG0380012186.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCAACTTACTATTTTAAG+AGG | + | Chr3:12385379-12385398 | None:intergenic | 20.0% |
| ! | AGAACAATTTAAATCCTGTT+TGG | - | Chr3:12385314-12385333 | MsG0380012186.01.T01:CDS | 25.0% |
| ! | ATTAAGGTTAATGTGGTTAA+AGG | - | Chr3:12385102-12385121 | MsG0380012186.01.T01:CDS | 25.0% |
| ! | CATCATTTGAAAAATAAGCT+TGG | - | Chr3:12385420-12385439 | MsG0380012186.01.T01:intron | 25.0% |
| ! | CTATGAGAAATCATCAATAA+TGG | - | Chr3:12385834-12385853 | MsG0380012186.01.T01:intron | 25.0% |
| ! | GAATAATTTCTCTTGAAGAA+GGG | - | Chr3:12385914-12385933 | MsG0380012186.01.T01:intron | 25.0% |
| ! | TTGAAAAATAAGCTTGGAAT+TGG | - | Chr3:12385426-12385445 | MsG0380012186.01.T01:intron | 25.0% |
| !! | ATTCAAAAACGACGATTTTA+TGG | - | Chr3:12385765-12385784 | MsG0380012186.01.T01:CDS | 25.0% |
| !! | CATTATTGATGATTTCTCAT+AGG | + | Chr3:12385836-12385855 | None:intergenic | 25.0% |
| !! | TTCAAAAACGACGATTTTAT+GGG | - | Chr3:12385766-12385785 | MsG0380012186.01.T01:CDS | 25.0% |
| !!! | AACTTACTATTTTAAGAGGA+GGG | + | Chr3:12385375-12385394 | None:intergenic | 25.0% |
| !!! | TGAATTTGTTGGATTGATTA+AGG | - | Chr3:12385086-12385105 | MsG0380012186.01.T01:CDS | 25.0% |
| AAATGATGTCTCACAGAAAA+TGG | + | Chr3:12385408-12385427 | None:intergenic | 30.0% | |
| AAGTTCCAAATGAATTCTAG+AGG | + | Chr3:12385506-12385525 | None:intergenic | 30.0% | |
| ACTTATCATAATGCTTGCAA+AGG | - | Chr3:12385222-12385241 | MsG0380012186.01.T01:CDS | 30.0% | |
| GGAATAATTTCTCTTGAAGA+AGG | - | Chr3:12385913-12385932 | MsG0380012186.01.T01:intron | 30.0% | |
| TCATATACAATCTGCATGTA+AGG | + | Chr3:12385736-12385755 | None:intergenic | 30.0% | |
| TCTAGCTACAAGTAAGATTA+AGG | + | Chr3:12385469-12385488 | None:intergenic | 30.0% | |
| TCTTACTTGTAGCTAGAATT+CGG | - | Chr3:12385472-12385491 | MsG0380012186.01.T01:intron | 30.0% | |
| TGGATTGATTAAGGTTAATG+TGG | - | Chr3:12385095-12385114 | MsG0380012186.01.T01:CDS | 30.0% | |
| TTATCAAACATGAAATCCAG+AGG | + | Chr3:12385700-12385719 | None:intergenic | 30.0% | |
| TTATTATCTGATCTTGCAGT+CGG | - | Chr3:12385273-12385292 | MsG0380012186.01.T01:CDS | 30.0% | |
| !! | CAACTTACTATTTTAAGAGG+AGG | + | Chr3:12385376-12385395 | None:intergenic | 30.0% |
| !!! | TTTTGCCTCTAGAATTCATT+TGG | - | Chr3:12385498-12385517 | MsG0380012186.01.T01:intron | 30.0% |
| ATTAGGCTTTCATTCCAAAC+AGG | + | Chr3:12385331-12385350 | None:intergenic | 35.0% | |
| CAAGAGAAAGGATGACATAA+GGG | + | Chr3:12385164-12385183 | None:intergenic | 35.0% | |
| CATGACTTAAAAGAAGAGCA+TGG | - | Chr3:12385667-12385686 | MsG0380012186.01.T01:intron | 35.0% | |
| GACAATACACTTGTGAAAGA+TGG | - | Chr3:12385892-12385911 | MsG0380012186.01.T01:intron | 35.0% | |
| TCACTATATCTCTGATAGCT+AGG | + | Chr3:12385134-12385153 | None:intergenic | 35.0% | |
| ! | TTGAATCAAGTGCGTGTTTT+TGG | + | Chr3:12385635-12385654 | None:intergenic | 35.0% |
| !! | CATGCTCTTCTTTTAAGTCA+TGG | + | Chr3:12385669-12385688 | None:intergenic | 35.0% |
| !!! | TAAGCTTGGAATTGGTCTTT+TGG | - | Chr3:12385434-12385453 | MsG0380012186.01.T01:intron | 35.0% |
| AGGCTACAAAATGTTGAGAG+AGG | - | Chr3:12385961-12385980 | MsG0380012186.01.T01:CDS | 40.0% | |
| AGTCAATGCAGCTTGGAAAA+TGG | - | Chr3:12385857-12385876 | MsG0380012186.01.T01:intron | 40.0% | |
| CCAAGAGAAAGGATGACATA+AGG | + | Chr3:12385165-12385184 | None:intergenic | 40.0% | |
| CCTTATGTCATCCTTTCTCT+TGG | - | Chr3:12385162-12385181 | MsG0380012186.01.T01:CDS | 40.0% | |
| GTCAATGCAGCTTGGAAAAT+GGG | - | Chr3:12385858-12385877 | MsG0380012186.01.T01:intron | 40.0% | |
| TGAGACAGGAAATCTCATTG+AGG | - | Chr3:12385941-12385960 | MsG0380012186.01.T01:CDS | 40.0% | |
| TTGACATGAAGAAACTCACC+TGG | + | Chr3:12385191-12385210 | None:intergenic | 40.0% | |
| ! | ATAATGGAGTCAATGCAGCT+TGG | - | Chr3:12385850-12385869 | MsG0380012186.01.T01:intron | 40.0% |
| ! | GCTTTTGCTGCTGATACTAA+TGG | + | Chr3:12385814-12385833 | None:intergenic | 40.0% |
| !! | TCTCAGGGATTGAAAGCATT+AGG | + | Chr3:12385348-12385367 | None:intergenic | 40.0% |
| !!! | AGTGCGTGTTTTTGGTTTTG+TGG | + | Chr3:12385627-12385646 | None:intergenic | 40.0% |
| CTTGGAAAATGGGTAGCAAG+TGG | - | Chr3:12385868-12385887 | MsG0380012186.01.T01:intron | 45.0% | |
| TAAGAGGAGGGATGTTCTCA+GGG | + | Chr3:12385363-12385382 | None:intergenic | 45.0% | |
| TGAAGAAGGGAAAGTGAGAC+AGG | - | Chr3:12385927-12385946 | MsG0380012186.01.T01:CDS | 45.0% | |
| TTAAGAGGAGGGATGTTCTC+AGG | + | Chr3:12385364-12385383 | None:intergenic | 45.0% | |
| AGAGCATGGTCTTCATCCTC+TGG | - | Chr3:12385681-12385700 | MsG0380012186.01.T01:intron | 50.0% | |
| CATCCTTTCTCTTGGTCACC+AGG | - | Chr3:12385170-12385189 | MsG0380012186.01.T01:CDS | 50.0% | |
| TCACCTGGTGACCAAGAGAA+AGG | + | Chr3:12385176-12385195 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 12385081 | 12386026 | 12385081 | ID=MsG0380012186.01;Name=MsG0380012186.01 |
| Chr3 | mRNA | 12385081 | 12386026 | 12385081 | ID=MsG0380012186.01.T01;Parent=MsG0380012186.01;Name=MsG0380012186.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|3|0|162 |
| Chr3 | exon | 12385916 | 12386026 | 12385916 | ID=MsG0380012186.01.T01:exon:2368;Parent=MsG0380012186.01.T01 |
| Chr3 | exon | 12385720 | 12385815 | 12385720 | ID=MsG0380012186.01.T01:exon:2367;Parent=MsG0380012186.01.T01 |
| Chr3 | exon | 12385081 | 12385362 | 12385081 | ID=MsG0380012186.01.T01:exon:2366;Parent=MsG0380012186.01.T01 |
| Chr3 | CDS | 12385916 | 12386026 | 12385916 | ID=MsG0380012186.01.T01:cds;Parent=MsG0380012186.01.T01 |
| Chr3 | CDS | 12385720 | 12385815 | 12385720 | ID=MsG0380012186.01.T01:cds;Parent=MsG0380012186.01.T01 |
| Chr3 | CDS | 12385081 | 12385362 | 12385081 | ID=MsG0380012186.01.T01:cds;Parent=MsG0380012186.01.T01 |
| Gene Sequence |
| Protein sequence |
