AlfalfaGEDB | MsG0280010525.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010525.01

KEGG: K02975
GO: -
PFAM: PF03297

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010525.01.T01	XP_003596834.1	100	107	0	0	1	107	1	107	7.03E-71	216

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010525.01.T01	Q9SIK2	91.667	108	8	1	1	107	1	108	2.37E-52	162

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010525.01.T01	B7FGM7	100.000	107	0	0	1	107	1	107	3.36e-71	216

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048687.01	MsG0280010525.01	0.832949	7.057279e-56	8.009235e-53
MsG0080048971.01	MsG0280010525.01	0.953843	1.118373e-111	3.019462e-106
MsG0180000575.01	MsG0280010525.01	0.833031	6.734094e-56	7.661628e-53
MsG0180003313.01	MsG0280010525.01	0.846264	2.422024e-59	4.145154e-56
MsG0180003729.01	MsG0280010525.01	0.804720	1.886561e-49	9.798713e-47
MsG0180003793.01	MsG0280010525.01	0.856060	4.151013e-62	9.783977e-59
MsG0180004009.01	MsG0280010525.01	0.804106	2.533150e-49	1.295011e-46
MsG0180004129.01	MsG0280010525.01	0.823174	1.596848e-53	1.364714e-50
MsG0180004130.01	MsG0280010525.01	0.815025	1.142080e-51	7.785640e-49
MsG0180004625.01	MsG0280010525.01	0.830697	2.537178e-55	2.693670e-52
MsG0180004854.01	MsG0280010525.01	0.833516	5.098818e-56	5.885907e-53
MsG0180005361.01	MsG0280010525.01	0.838962	2.105313e-57	2.865136e-54
MsG0180005366.01	MsG0280010525.01	0.835559	1.563839e-56	1.919155e-53
MsG0180005426.01	MsG0280010525.01	0.850526	1.603708e-60	3.150242e-57
MsG0280006424.01	MsG0280010525.01	0.809396	1.931554e-50	1.132845e-47
MsG0280006819.01	MsG0280010525.01	0.821024	5.031080e-53	4.046594e-50
MsG0280006836.01	MsG0280010525.01	0.877064	8.474908e-69	4.204014e-65
MsG0280006993.01	MsG0280010525.01	0.850702	1.430973e-60	2.827302e-57
MsG0280008118.01	MsG0280010525.01	0.843811	1.113526e-58	1.762672e-55
MsG0280009372.01	MsG0280010525.01	0.801032	1.090097e-48	5.153193e-46
MsG0280009375.01	MsG0280010525.01	0.837213	5.936234e-57	7.657074e-54
MsG0280010495.01	MsG0280010525.01	0.858618	7.278030e-63	1.868725e-59
MsG0280010525.01	MsG0280010801.01	0.829869	4.043893e-55	4.191710e-52
MsG0280010525.01	MsG0280010995.01	0.803181	3.940670e-49	1.967240e-46
MsG0280010525.01	MsG0280011234.01	0.852092	5.791357e-61	1.196927e-57
MsG0280010525.01	MsG0380012073.01	0.872140	3.997379e-67	1.654145e-63
MsG0280010525.01	MsG0380016389.01	0.808046	3.753678e-50	2.124174e-47
MsG0280010525.01	MsG0380016778.01	0.830153	3.448013e-55	3.603050e-52
MsG0280010525.01	MsG0380017382.01	0.842815	2.053847e-58	3.150759e-55
MsG0280010525.01	MsG0480018084.01	0.829150	6.047785e-55	6.137391e-52
MsG0280010525.01	MsG0480018477.01	0.841492	4.597798e-58	6.767407e-55
MsG0280010525.01	MsG0480021006.01	0.811452	6.953807e-51	4.306015e-48
MsG0280010525.01	MsG0480021159.01	0.855156	7.622156e-62	1.743130e-58
MsG0280010525.01	MsG0480023149.01	0.841436	4.758580e-58	6.992068e-55
MsG0280010525.01	MsG0480023399.01	0.801614	8.285755e-49	3.974444e-46
MsG0280010525.01	MsG0480023467.01	0.860855	1.543567e-63	4.280146e-60
MsG0280010525.01	MsG0480023771.01	0.817575	3.070997e-52	2.244808e-49
MsG0280010525.01	MsG0580024369.01	0.848588	5.569866e-60	1.027120e-56
MsG0280010525.01	MsG0580026157.01	0.802107	6.564435e-49	3.188485e-46
MsG0280010525.01	MsG0580027043.01	0.827538	1.480761e-54	1.433595e-51
MsG0280010525.01	MsG0580029132.01	0.863593	2.228132e-64	6.791026e-61
MsG0280010525.01	MsG0680032770.01	0.837793	4.213940e-57	5.534491e-54
MsG0280010525.01	MsG0680035848.01	0.813391	2.620779e-51	1.709391e-48
MsG0280010525.01	MsG0780036240.01	0.805763	1.141009e-49	6.087457e-47
MsG0280010525.01	MsG0780036822.01	0.853122	2.943622e-61	6.292644e-58
MsG0280010525.01	MsG0780038400.01	0.850777	1.363480e-60	2.700557e-57
MsG0280010525.01	MsG0780038587.01	0.842761	2.123069e-58	3.251309e-55
MsG0280010525.01	MsG0780040782.01	0.839715	1.342252e-57	1.869367e-54
MsG0280010525.01	MsG0780040900.01	0.854060	1.582154e-61	3.490492e-58
MsG0280010525.01	MsG0780041147.01	0.889235	2.905568e-73	2.324412e-69
MsG0280010525.01	MsG0780041223.01	0.838019	3.687603e-57	4.876876e-54
MsG0280010525.01	MsG0780041224.01	0.838434	2.881948e-57	3.859824e-54
MsG0280010525.01	MsG0780041332.01	0.833868	4.165607e-56	4.859752e-53
MsG0280010525.01	MsG0780041519.01	0.805479	1.308467e-49	6.929816e-47
MsG0280010525.01	MsG0880041967.01	0.846018	2.827280e-59	4.800968e-56
MsG0280010525.01	MsG0880042125.01	0.853219	2.761220e-61	5.922853e-58
MsG0280010525.01	MsG0880042991.01	0.804257	2.356426e-49	1.209277e-46
MsG0280010525.01	MsG0880043517.01	0.802001	6.901580e-49	3.343198e-46
MsG0280010525.01	MsG0880043770.01	0.808345	3.242360e-50	1.849429e-47
MsG0280010525.01	MsG0880046177.01	0.802688	4.981152e-49	2.455621e-46
MsG0280010525.01	MsG0880046444.01	0.876943	9.334336e-69	4.610254e-65
MsG0280010525.01	MsG0880046879.01	0.818260	2.150394e-52	1.601690e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010525.01.T01	MTR_2g086610	100.000	107	0	0	1	107	1	107	8.51e-75	216
MsG0280010525.01.T01	MTR_4g070600	99.065	107	1	0	1	107	1	107	2.67e-74	215
MsG0280010525.01.T01	MTR_4g070600	98.131	107	1	1	1	107	1	106	7.53e-72	209
MsG0280010525.01.T01	MTR_6g015615	80.556	72	4	2	2	72	13	75	2.70e-17	70.9
MsG0280010525.01.T01	MTR_3g117040	80.556	72	4	2	2	72	13	75	2.70e-17	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010525.01.T01	AT2G21580	91.667	108	8	1	1	107	1	108	2.41e-53	162
MsG0280010525.01.T01	AT4G39200	88.889	108	11	1	1	107	1	108	9.21e-52	158
MsG0280010525.01.T01	AT2G21580	87.963	108	11	2	1	107	1	107	1.09e-51	158
MsG0280010525.01.T01	AT4G39200	87.037	108	12	2	1	107	1	107	4.24e-50	154
MsG0280010525.01.T01	AT4G34555	88.785	107	11	1	1	106	1	107	9.73e-50	153
MsG0280010525.01.T01	AT2G16360	85.714	105	14	1	2	105	2	106	3.03e-46	144

Find 41 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGAGTGATGAGTTTGTATT+TGG	0.207961	2:-70933975	None:intergenic
TGCTAGCCGCAGAGAAGATT+TGG	0.298822	2:-70933671	None:intergenic
GAAAGCAAAAGAAGAAGAAA+TGG	0.344636	2:+70933870	MsG0280010525.01.T01:CDS
GGGGTTGGTGGTTACCATTA+GGG	0.358777	2:-70933710	None:intergenic
CAGTCCTCTCTGATCGTCTC+AGG	0.361688	2:+70933999	MsG0280010525.01.T01:CDS
GAAGATTTGGATTTGGAAGA+AGG	0.363786	2:-70933658	None:intergenic
CATTATTATGAGGATGTGTT+TGG	0.370583	2:+70934264	MsG0280010525.01.T01:three_prime_UTR
CCGCAGAGAAGATTTGGATT+TGG	0.380715	2:-70933665	None:intergenic
TGATGGCAAGAGGATTGATT+AGG	0.387796	2:+70934142	MsG0280010525.01.T01:CDS
TCATCAAAGCCGGCGAAATC+AGG	0.410202	2:+70933842	MsG0280010525.01.T01:CDS
GGTGGTGGTGCTGCCTTCTT+CGG	0.414824	2:-70933818	None:intergenic
TCGTGCAATCAGAGATTTGA+TGG	0.416147	2:+70934125	MsG0280010525.01.T01:CDS
TGAAACCGCCTATGTATTAG+TGG	0.440250	2:-70934205	None:intergenic
AAGATTTGGATTTGGAAGAA+GGG	0.455542	2:-70933657	None:intergenic
TCATCAACTTCAAACCCTAA+TGG	0.458152	2:+70933696	MsG0280010525.01.T01:exon
CTAATACATAGGCGGTTTCA+AGG	0.473470	2:+70934208	MsG0280010525.01.T01:exon
AACATGGTCTTGTTTGATCA+AGG	0.477202	2:+70933923	MsG0280010525.01.T01:CDS
CAAAGGTAAACAAAAGGAAA+AGG	0.503611	2:+70933895	MsG0280010525.01.T01:CDS
TTCAGTGAGAAGCTTATCAT+AGG	0.513766	2:-70933948	None:intergenic
GATTTCGCCGGCTTTGATGA+CGG	0.516878	2:-70933839	None:intergenic
GGGTTTGTTCTCAATAACTC+AGG	0.519467	2:-70933637	None:intergenic
TTAGAGACAACATTATTATG+AGG	0.522671	2:+70934254	MsG0280010525.01.T01:three_prime_UTR
ATGGAGCAAAGGTAAACAAA+AGG	0.525529	2:+70933889	MsG0280010525.01.T01:CDS
ACCACCACCGTCATCAAAGC+CGG	0.527384	2:+70933832	MsG0280010525.01.T01:CDS
CACTAGAGCCACTAATACAT+AGG	0.531061	2:+70934197	MsG0280010525.01.T01:CDS
TTTGTTGTAGGCACCGAAGA+AGG	0.534954	2:+70933805	MsG0280010525.01.T01:intron
GGAGTGATGAGTTTGTATTT+GGG	0.540872	2:-70933974	None:intergenic
ATCAGAGATTTGATGGCAAG+AGG	0.560553	2:+70934132	MsG0280010525.01.T01:CDS
TTTCCTCCTCCTGATTTCGC+CGG	0.569055	2:-70933851	None:intergenic
GCCGGCTTTGATGACGGTGG+TGG	0.569126	2:-70933833	None:intergenic
AGACGATCAGAGAGGACTGA+AGG	0.570313	2:-70933995	None:intergenic
GGCAAGAGGATTGATTAGGT+TGG	0.587429	2:+70934146	MsG0280010525.01.T01:CDS
AGTCCTCTCTGATCGTCTCA+GGG	0.592148	2:+70934000	MsG0280010525.01.T01:CDS
AAGAAGAAGAAATGGAGCAA+AGG	0.611863	2:+70933878	MsG0280010525.01.T01:CDS
AAGCCGGCGAAATCAGGAGG+AGG	0.614520	2:+70933848	MsG0280010525.01.T01:CDS
TCAAAGCCGGCGAAATCAGG+AGG	0.640955	2:+70933845	MsG0280010525.01.T01:CDS
AAAGGAAAAGGTTAACAACA+TGG	0.683005	2:+70933907	MsG0280010525.01.T01:CDS
CCAAATCCAAATCTTCTCTG+CGG	0.707561	2:+70933665	MsG0280010525.01.T01:five_prime_UTR
TTCGCCGGCTTTGATGACGG+TGG	0.725481	2:-70933836	None:intergenic
TAGAGCCACTAATACATAGG+CGG	0.728751	2:+70934200	MsG0280010525.01.T01:exon
ATACCCTGAGACGATCAGAG+AGG	0.757534	2:-70934003	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGTAATGTTTTCAGATTAA+TGG	+	Chr2:70934090-70934109	MsG0280010525.01.T01:intron	20.0%
!	TTAGAGACAACATTATTATG+AGG	+	Chr2:70934254-70934273	MsG0280010525.01.T01:three_prime_UTR	25.0%
!!!	TTTTCAGATTAATGGATCTT+TGG	+	Chr2:70934098-70934117	MsG0280010525.01.T01:intron	25.0%
	AAAGGAAAAGGTTAACAACA+TGG	+	Chr2:70933907-70933926	MsG0280010525.01.T01:CDS	30.0%
	AAGATTTGGATTTGGAAGAA+GGG	-	Chr2:70933660-70933679	None:intergenic	30.0%
	CAAAGGTAAACAAAAGGAAA+AGG	+	Chr2:70933895-70933914	MsG0280010525.01.T01:CDS	30.0%
	CATTATTATGAGGATGTGTT+TGG	+	Chr2:70934264-70934283	MsG0280010525.01.T01:three_prime_UTR	30.0%
	GAAAGCAAAAGAAGAAGAAA+TGG	+	Chr2:70933870-70933889	MsG0280010525.01.T01:CDS	30.0%
	TAAGGAAAGGAATTTGATGA+TGG	-	Chr2:70933741-70933760	None:intergenic	30.0%
!!	ACTTCCATTTTCTTTGTTGT+AGG	+	Chr2:70933793-70933812	MsG0280010525.01.T01:intron	30.0%
!!	AGGAATTTGATGATGGAAAT+TGG	-	Chr2:70933734-70933753	None:intergenic	30.0%
!!	GAATTTGATGATGGAAATTG+GGG	-	Chr2:70933732-70933751	None:intergenic	30.0%
!!	GGAATTTGATGATGGAAATT+GGG	-	Chr2:70933733-70933752	None:intergenic	30.0%
	AAGAAGAAGAAATGGAGCAA+AGG	+	Chr2:70933878-70933897	MsG0280010525.01.T01:CDS	35.0%
	AGGAGTGATGAGTTTGTATT+TGG	-	Chr2:70933978-70933997	None:intergenic	35.0%
	ATGGAGCAAAGGTAAACAAA+AGG	+	Chr2:70933889-70933908	MsG0280010525.01.T01:CDS	35.0%
	CGAGATTTAGATCTAAGGAA+AGG	-	Chr2:70933754-70933773	None:intergenic	35.0%
	GAAGATTTGGATTTGGAAGA+AGG	-	Chr2:70933661-70933680	None:intergenic	35.0%
	GGAGTGATGAGTTTGTATTT+GGG	-	Chr2:70933977-70933996	None:intergenic	35.0%
	TCATCAACTTCAAACCCTAA+TGG	+	Chr2:70933696-70933715	MsG0280010525.01.T01:exon	35.0%
	TGCAACGAGATTTAGATCTA+AGG	-	Chr2:70933759-70933778	None:intergenic	35.0%
	TTCAGTGAGAAGCTTATCAT+AGG	-	Chr2:70933951-70933970	None:intergenic	35.0%
!!	AACATGGTCTTGTTTGATCA+AGG	+	Chr2:70933923-70933942	MsG0280010525.01.T01:CDS	35.0%
	CACTAGAGCCACTAATACAT+AGG	+	Chr2:70934197-70934216	MsG0280010525.01.T01:CDS	40.0%
	CCAAATCCAAATCTTCTCTG+CGG	+	Chr2:70933665-70933684	MsG0280010525.01.T01:five_prime_UTR	40.0%
	CTAATACATAGGCGGTTTCA+AGG	+	Chr2:70934208-70934227	MsG0280010525.01.T01:exon	40.0%
	GGTGCCTACAACAAAGAAAA+TGG	-	Chr2:70933800-70933819	None:intergenic	40.0%
	TAGAGCCACTAATACATAGG+CGG	+	Chr2:70934200-70934219	MsG0280010525.01.T01:exon	40.0%
	TCGTGCAATCAGAGATTTGA+TGG	+	Chr2:70934125-70934144	MsG0280010525.01.T01:CDS	40.0%
	TGAAACCGCCTATGTATTAG+TGG	-	Chr2:70934208-70934227	None:intergenic	40.0%
	TGAATGAATCGAAGCGACAA+AGG	-	Chr2:70934054-70934073	None:intergenic	40.0%
	TGATGGCAAGAGGATTGATT+AGG	+	Chr2:70934142-70934161	MsG0280010525.01.T01:CDS	40.0%
!	ATCAGAGATTTGATGGCAAG+AGG	+	Chr2:70934132-70934151	MsG0280010525.01.T01:CDS	40.0%
!!	TTGATGATGGAAATTGGGGT+TGG	-	Chr2:70933728-70933747	None:intergenic	40.0%
	CCGCAGAGAAGATTTGGATT+TGG	-	Chr2:70933668-70933687	None:intergenic	45.0%
	GGCAAGAGGATTGATTAGGT+TGG	+	Chr2:70934146-70934165	MsG0280010525.01.T01:CDS	45.0%
!	ATGATGGAAATTGGGGTTGG+TGG	-	Chr2:70933725-70933744	None:intergenic	45.0%
!!	TTTGTTGTAGGCACCGAAGA+AGG	+	Chr2:70933805-70933824	MsG0280010525.01.T01:intron	45.0%
	AGACGATCAGAGAGGACTGA+AGG	-	Chr2:70933998-70934017	None:intergenic	50.0%
	AGTCCTCTCTGATCGTCTCA+GGG	+	Chr2:70934000-70934019	MsG0280010525.01.T01:CDS	50.0%
	ATACCCTGAGACGATCAGAG+AGG	-	Chr2:70934006-70934025	None:intergenic	50.0%
	TCATCAAAGCCGGCGAAATC+AGG	+	Chr2:70933842-70933861	MsG0280010525.01.T01:CDS	50.0%
	TGCTAGCCGCAGAGAAGATT+TGG	-	Chr2:70933674-70933693	None:intergenic	50.0%
	TTTCCTCCTCCTGATTTCGC+CGG	-	Chr2:70933854-70933873	None:intergenic	50.0%
!!	GATTTCGCCGGCTTTGATGA+CGG	-	Chr2:70933842-70933861	None:intergenic	50.0%
!!	GGGGTTGGTGGTTACCATTA+GGG	-	Chr2:70933713-70933732	None:intergenic	50.0%
!!	TGGGGTTGGTGGTTACCATT+AGG	-	Chr2:70933714-70933733	None:intergenic	50.0%
	ACCACCACCGTCATCAAAGC+CGG	+	Chr2:70933832-70933851	MsG0280010525.01.T01:CDS	55.0%
	CAGTCCTCTCTGATCGTCTC+AGG	+	Chr2:70933999-70934018	MsG0280010525.01.T01:CDS	55.0%
	TCAAAGCCGGCGAAATCAGG+AGG	+	Chr2:70933845-70933864	MsG0280010525.01.T01:CDS	55.0%
	AAGCCGGCGAAATCAGGAGG+AGG	+	Chr2:70933848-70933867	MsG0280010525.01.T01:CDS	60.0%
!!	GGTGGTGGTGCTGCCTTCTT+CGG	-	Chr2:70933821-70933840	None:intergenic	60.0%
!!	TTCGCCGGCTTTGATGACGG+TGG	-	Chr2:70933839-70933858	None:intergenic	60.0%
!!	GCCGGCTTTGATGACGGTGG+TGG	-	Chr2:70933836-70933855	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	70933640	70934388	70933640	ID=MsG0280010525.01;Name=MsG0280010525.01
Chr2	mRNA	70933640	70934388	70933640	ID=MsG0280010525.01.T01;Parent=MsG0280010525.01;Name=MsG0280010525.01.T01;_AED=0.45;_eAED=0.45;_QI=75\|1\|1\|1\|1\|1\|3\|170\|107
Chr2	exon	70933640	70933717	70933640	ID=MsG0280010525.01.T01:exon:10062;Parent=MsG0280010525.01.T01
Chr2	exon	70933815	70934021	70933815	ID=MsG0280010525.01.T01:exon:10063;Parent=MsG0280010525.01.T01
Chr2	exon	70934105	70934388	70934105	ID=MsG0280010525.01.T01:exon:10064;Parent=MsG0280010525.01.T01
Chr2	five_prime_UTR	70933640	70933714	70933640	ID=MsG0280010525.01.T01:five_prime_utr;Parent=MsG0280010525.01.T01
Chr2	CDS	70933715	70933717	70933715	ID=MsG0280010525.01.T01:cds;Parent=MsG0280010525.01.T01
Chr2	CDS	70933815	70934021	70933815	ID=MsG0280010525.01.T01:cds;Parent=MsG0280010525.01.T01
Chr2	CDS	70934105	70934218	70934105	ID=MsG0280010525.01.T01:cds;Parent=MsG0280010525.01.T01
Chr2	three_prime_UTR	70934219	70934388	70934219	ID=MsG0280010525.01.T01:three_prime_utr;Parent=MsG0280010525.01.T01

Gene Sequence	>MsG0280010525.01.T01 ATGGCACCGAAGAAGGCAGCACCACCACCGTCATCAAAGCCGGCGAAATCAGGAGGAGGAAAGCAAAAGAAGAAGAAATGGAGCAAAGGTAAACAAAAGGAAAAGGTTAACAACATGGTCTTGTTTGATCAAGGTACCTATGATAAGCTTCTCACTGAAGCTCCCAAATACAAACTCATCACTCCTTCAGTCCTCTCTGATCGTCTCAGGATTAATGGATCTTTGGCACGTCGTGCAATCAGAGATTTGATGGCAAGAGGATTGATTAGGTTGGTGTCTGCTCATTCTAGTCAGCAGATTTACACTAGAGCCACTAATACATAG
Protein sequence	>MsG0280010525.01.T01 MAPKKAAPPPSSKPAKSGGGKQKKKKWSKGKQKEKVNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMARGLIRLVSAHSSQQIYTRATNT*