AlfalfaGEDB | MsG0380013963.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013963.01

KEGG: K20000
GO: -
PFAM: PF01824

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013963.01.T02	AFC82136.1	100	112	0	38	149	190	301	8.75E-72	228
MsG0380013963.01.T03	AFC82136.1	100	98	0	1	98	204	301	1.14E-61	200
MsG0380013963.01.T01	YP_009141593.1	99.401	334	2	1	334	1	334	0	671

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013963.01.T01	Q9TKI5	97.904	334	7	1	334	1	334	0	659
MsG0380013963.01.T02	Q8MCP6	98.214	112	2	38	149	334	445	3.01E-72	228
MsG0380013963.01.T03	Q8MCP6	97.959	98	2	1	98	348	445	2.51E-62	200

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013963.01.T01	D9IPA2	99.401	334	2	1	334	1	334	0.0	671
MsG0380013963.01.T02	W8CKI2	100.000	112	0	38	149	334	445	1.50e-70	230
MsG0380013963.01.T03	H9APK0	100.000	98	0	1	98	204	301	5.47e-62	200

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047910.01	MsG0380013963.01	0.801488	8.794289e-49	4.205152e-46
MsG0080048689.01	MsG0380013963.01	0.809592	1.753406e-50	1.033749e-47
MsG0080049077.01	MsG0380013963.01	0.823237	1.543568e-53	1.321505e-50
MsG0080049078.01	MsG0380013963.01	0.815746	7.895425e-52	5.490026e-49
MsG0080049080.01	MsG0380013963.01	0.803931	2.754445e-49	1.401928e-46
MsG0180000325.01	MsG0380013963.01	0.803112	4.072154e-49	2.029212e-46
MsG0180001919.01	MsG0380013963.01	0.803565	3.280429e-49	1.654005e-46
MsG0180003933.01	MsG0380013963.01	0.819441	1.159459e-52	8.923152e-50
MsG0180004302.01	MsG0380013963.01	0.811978	5.341199e-51	3.353997e-48
MsG0180004937.01	MsG0380013963.01	0.810879	9.254599e-51	5.644937e-48
MsG0180005879.01	MsG0380013963.01	0.829675	4.506811e-55	4.645025e-52
MsG0280006336.01	MsG0380013963.01	0.809814	1.570777e-50	9.315138e-48
MsG0380012065.01	MsG0380013963.01	0.807374	5.216315e-50	2.900485e-47
MsG0380012443.01	MsG0380013963.01	0.833490	5.177000e-56	5.971119e-53
MsG0380012444.01	MsG0380013963.01	0.808664	2.771648e-50	1.594072e-47
MsG0380013888.01	MsG0380013963.01	0.816792	4.607943e-52	3.296838e-49
MsG0380013889.01	MsG0380013963.01	0.834098	3.648053e-56	4.285215e-53
MsG0380013918.01	MsG0380013963.01	0.828022	1.132351e-54	1.112028e-51
MsG0380013919.01	MsG0380013963.01	0.832009	1.206681e-55	1.331824e-52
MsG0380013925.01	MsG0380013963.01	0.809359	1.967694e-50	1.152916e-47
MsG0380013926.01	MsG0380013963.01	0.805821	1.109126e-49	5.926127e-47
MsG0380013927.01	MsG0380013963.01	0.808922	2.440891e-50	1.413436e-47
MsG0380013930.01	MsG0380013963.01	0.810427	1.158747e-50	6.982619e-48
MsG0380013932.01	MsG0380013963.01	0.832452	9.376617e-56	1.048443e-52
MsG0380013935.01	MsG0380013963.01	0.815493	8.986959e-52	6.205771e-49
MsG0380013938.01	MsG0380013963.01	0.815599	8.510695e-52	5.893988e-49
MsG0380013939.01	MsG0380013963.01	0.845767	3.307724e-59	5.571850e-56
MsG0380013955.01	MsG0380013963.01	0.805552	1.263481e-49	6.703840e-47
MsG0380013958.01	MsG0380013963.01	0.846077	2.723943e-59	4.633942e-56
MsG0380013959.01	MsG0380013963.01	0.801876	7.321827e-49	3.535778e-46
MsG0380013963.01	MsG0380013964.01	0.844733	6.296116e-59	1.025999e-55
MsG0380013963.01	MsG0380013970.01	0.800984	1.115152e-48	5.265240e-46
MsG0380013963.01	MsG0380013972.01	0.808549	2.932063e-50	1.681392e-47
MsG0380013963.01	MsG0380013976.01	0.809601	1.745933e-50	1.029597e-47
MsG0380013963.01	MsG0380013979.01	0.843672	1.213134e-58	1.912146e-55
MsG0380013963.01	MsG0380013980.01	0.829238	5.756634e-55	5.857727e-52
MsG0380013963.01	MsG0380013981.01	0.830889	2.277392e-55	2.431527e-52
MsG0380013963.01	MsG0380013983.01	0.835716	1.426917e-56	1.759566e-53
MsG0380013963.01	MsG0380013992.01	0.810061	1.390231e-50	8.297868e-48
MsG0380013963.01	MsG0380013996.01	0.829474	5.045948e-55	5.169582e-52
MsG0380013963.01	MsG0380014022.01	0.820661	6.096105e-53	4.853100e-50
MsG0380013963.01	MsG0380014028.01	0.923376	2.881831e-89	1.148252e-84
MsG0380013963.01	MsG0380014034.01	0.820915	5.328312e-53	4.272491e-50
MsG0380013963.01	MsG0380014035.01	0.844378	7.846430e-59	1.264291e-55
MsG0380013963.01	MsG0380014036.01	0.815596	8.524861e-52	5.903216e-49
MsG0380013963.01	MsG0380014037.01	0.836532	8.854064e-57	1.118904e-53
MsG0380013963.01	MsG0380014048.01	0.802909	4.485239e-49	2.223657e-46
MsG0380013963.01	MsG0380014050.01	0.830737	2.481864e-55	2.637940e-52
MsG0380013963.01	MsG0380014053.01	0.825249	5.195058e-54	4.709939e-51
MsG0380013963.01	MsG0380015530.01	0.820316	7.316755e-53	5.769711e-50
MsG0380013963.01	MsG0380015733.01	0.817836	2.682152e-52	1.974747e-49
MsG0380013963.01	MsG0380016531.01	0.817998	2.464716e-52	1.822734e-49
MsG0380013963.01	MsG0380016751.01	0.803190	3.924142e-49	1.959467e-46
MsG0380013963.01	MsG0480022296.01	0.809867	1.530132e-50	9.086546e-48
MsG0380013963.01	MsG0480023881.01	0.818601	1.800411e-52	1.353698e-49
MsG0380013963.01	MsG0580024982.01	0.817090	3.949223e-52	2.848702e-49
MsG0380013963.01	MsG0580027249.01	0.800252	1.572814e-48	7.288926e-46
MsG0380013963.01	MsG0580029673.01	0.800385	1.477653e-48	6.871171e-46
MsG0380013963.01	MsG0780038496.01	0.817298	3.546147e-52	2.572521e-49
MsG0380013963.01	MsG0780039596.01	0.813101	3.035800e-51	1.964234e-48
MsG0380013963.01	MsG0780039859.01	0.832914	7.203570e-56	8.166371e-53
MsG0380013963.01	MsG0780039860.01	0.811111	8.243804e-51	5.059173e-48
MsG0380013963.01	MsG0780040315.01	0.838192	3.327761e-57	4.423573e-54
MsG0380013963.01	MsG0780040316.01	0.832590	8.667359e-56	9.731585e-53
MsG0380013963.01	MsG0780040317.01	0.820925	5.302335e-53	4.252710e-50
MsG0380013963.01	MsG0780040644.01	0.806035	9.999914e-50	5.372356e-47
MsG0380013963.01	MsG0780041106.01	0.801281	9.695450e-49	4.611983e-46
MsG0380013963.01	MsG0780041296.01	0.809356	1.970792e-50	1.154635e-47
MsG0380013963.01	MsG0880043032.01	0.812914	3.335486e-51	2.147463e-48
MsG0380013963.01	MsG0880043541.01	0.826354	2.841174e-54	2.658198e-51
MsG0380013963.01	MsG0880044312.01	0.834929	2.255871e-56	2.716785e-53
MsG0380013963.01	MsG0880044352.01	0.802512	5.416062e-49	2.658014e-46
MsG0380013963.01	MsG0880044440.01	0.838748	2.392218e-57	3.234439e-54
MsG0380013963.01	MsG0880045252.01	0.820848	5.522366e-53	4.419710e-50
MsG0380013963.01	MsG0880045283.01	0.820399	7.001387e-53	5.534164e-50
MsG0380013963.01	MsG0880045412.01	0.839946	1.168633e-57	1.639516e-54
MsG0280007041.01	MsG0380013963.01	0.847839	8.967138e-60	1.614362e-56
MsG0280007042.01	MsG0380013963.01	0.842710	2.190337e-58	3.349088e-55

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013963.01.T02	MTR_3g035685	99.107	112	1	0	38	149	334	445	1.16e-73	229
MsG0380013963.01.T02	MTR_7g021760	99.107	112	1	0	38	149	317	428	1.34e-73	228
MsG0380013963.01.T02	MTR_4g051280	82.143	112	2	1	38	149	334	427	1.62e-54	179
MsG0380013963.01.T02	MTR_0002s0390	66.019	103	35	0	47	149	347	449	8.11e-42	145
MsG0380013963.01.T02	MTR_4g079120	96.970	33	1	0	43	75	3	35	9.84e-15	65.1
MsG0380013963.01.T02	MTR_2g089570	90.625	32	3	0	38	69	17	48	3.64e-12	58.2
MsG0380013963.01.T03	MTR_3g035685	98.980	98	1	0	1	98	348	445	9.85e-64	201
MsG0380013963.01.T03	MTR_7g021760	98.980	98	1	0	1	98	331	428	1.36e-63	200
MsG0380013963.01.T03	MTR_4g051280	79.592	98	2	1	1	98	348	427	5.68e-45	151
MsG0380013963.01.T03	MTR_0002s0390	66.327	98	33	0	1	98	352	449	1.13e-39	137
MsG0380013963.01.T01	MTR_4g051280	96.108	334	13	0	1	334	1	334	0.0	648
MsG0380013963.01.T01	MTR_3g035685	96.108	334	13	0	1	334	1	334	0.0	647
MsG0380013963.01.T01	MTR_7g021760	96.440	309	11	0	1	309	1	309	0.0	597
MsG0380013963.01.T01	MTR_0002s0390	56.932	339	138	5	1	333	1	337	2.49e-112	336
MsG0380013963.01.T01	MTR_7g117940	95.192	104	5	0	1	104	1	104	3.51e-67	206
MsG0380013963.01.T01	MTR_7g098060	92.523	107	8	0	178	284	26	132	2.47e-57	182
MsG0380013963.01.T01	MTR_2g089600	95.000	60	3	0	160	219	1	60	2.27e-33	118

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013963.01.T02	ATCG00040	70.000	110	33	0	40	149	357	466	5.06e-46	157
MsG0380013963.01.T03	ATCG00040	71.429	98	28	0	1	98	369	466	1.04e-41	143
MsG0380013963.01.T01	ATCG00040	57.522	339	133	3	1	334	23	355	2.41e-126	372

Find 42 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAATGAATGTTCGAATAAT+TGG	0.184187	3:+48523256	None:intergenic
TGTAAATTTATCCTCCAAAA+AGG	0.215684	3:+48523818	None:intergenic
AGGATTTATTACGTAGAAAA+AGG	0.217646	3:+48523550	None:intergenic
GATCTAATCAGAGGAATAAT+TGG	0.252243	3:+48523121	None:intergenic
TAGTTCAAATCCTTCGATAC+TGG	0.264029	3:-48523740	MsG0380013963.01.T01:CDS
TTCTTATTATAGCCCCAAAA+TGG	0.277111	3:+48523978	None:intergenic
ACAGAATCATTTAATCATTT+CGG	0.283385	3:-48524021	MsG0380013963.01.T01:CDS
CTAAGGAACAGCTTAATTCT+AGG	0.309400	3:+48523898	None:intergenic
TATGATTTCTGCCTCCTCTA+AGG	0.324557	3:+48523881	None:intergenic
TATTCCTCTGATTAGATCAT+TGG	0.324715	3:-48523116	MsG0380013963.01.T02:CDS
TGGTTTATATTGATCGTTCT+TGG	0.359317	3:+48523276	None:intergenic
ATCCTATTAGTAAGCCGGTC+TGG	0.371851	3:-48523063	MsG0380013963.01.T02:CDS
TCGGTCAATAATATCAAAAT+CGG	0.400724	3:+48523030	None:intergenic
ATTCATTATGTTAGATATCA+AGG	0.403164	3:-48523399	MsG0380013963.01.T01:CDS
ATATCAAGGAAAATGCATTC+TGG	0.408653	3:-48523385	MsG0380013963.01.T01:CDS
GTATACTTTAAGATTCTTCA+AGG	0.409069	3:-48523430	MsG0380013963.01.T01:CDS
GCTTTCTTGTATTAAAACTT+TGG	0.413392	3:-48522921	MsG0380013963.01.T02:CDS
AGAATTAAGCTGTTCCTTAG+AGG	0.417803	3:-48523895	MsG0380013963.01.T01:CDS
ATTTCCAGATGGATAGGATA+TGG	0.418289	3:+48523765	None:intergenic
GCCCAGACCGGCTTACTAAT+AGG	0.422623	3:+48523061	None:intergenic
TCCTATTAGTAAGCCGGTCT+GGG	0.425073	3:-48523062	MsG0380013963.01.T02:CDS
AATATCAAAATCGGATGAAT+CGG	0.430249	3:+48523039	None:intergenic
AGTTCAAATCCTTCGATACT+GGG	0.471152	3:-48523739	MsG0380013963.01.T01:CDS
AATACCATATCCTATCCATC+TGG	0.476500	3:-48523769	MsG0380013963.01.T01:CDS
ACGAGCTAGATCTCGCCAAC+AGG	0.482502	3:-48524192	MsG0380013963.01.T01:CDS
AATCTTTCTCATTATTACAA+TGG	0.508907	3:-48522989	MsG0380013963.01.T02:CDS
GGGTAAATCGTTCAGTGGTA+CGG	0.514387	3:-48523207	MsG0380013963.01.T02:CDS
ATCGAATAAAATATATACTT+CGG	0.528810	3:-48522943	MsG0380013963.01.T02:CDS
ACTAAGATTTCCAGATGGAT+AGG	0.531965	3:+48523759	None:intergenic
TAGAGGAACAAGAATAATCT+CGG	0.549524	3:+48523606	None:intergenic
AGGGCATCCTATTAGTAAGC+CGG	0.566008	3:-48523068	MsG0380013963.01.T02:CDS
ATTAAGCTGTTCCTTAGAGG+AGG	0.579200	3:-48523892	MsG0380013963.01.T01:CDS
TCGGATGAATCGGCCCAGAC+CGG	0.580873	3:+48523049	None:intergenic
CATCTTTCACCCAGTATCGA+AGG	0.584400	3:+48523730	None:intergenic
TTATTCTCAAATAATATCAG+AGG	0.589909	3:-48523952	MsG0380013963.01.T01:CDS
TATTCTCAAATAATATCAGA+GGG	0.595885	3:-48523951	MsG0380013963.01.T01:CDS
TTTGAACTAAGATTTCCAGA+TGG	0.614536	3:+48523754	None:intergenic
AAATGGAATTTCCACGACGA+TGG	0.619896	3:+48523923	None:intergenic
TATCTAACATAATGAATGAG+AGG	0.620526	3:+48523405	None:intergenic
TTAGCCAATGATCTAATCAG+AGG	0.639023	3:+48523112	None:intergenic
GTGGGTATAGGAAGTCCTGT+TGG	0.646926	3:+48524177	None:intergenic
TGTGCGGGTAAATCGTTCAG+TGG	0.687547	3:-48523212	MsG0380013963.01.T02:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AACATTATTTTATTCATTTA+TGG	-	Chr3:48523778-48523797	MsG0380013963.01.T01:CDS	10.0%
!!!	TTCTTTATAATTTTAGTAAT+TGG	-	Chr3:48523424-48523443	MsG0380013963.01.T01:CDS	10.0%
!!	AATCGTAATAAATGAAAAAA+AGG	+	Chr3:48523402-48523421	None:intergenic	15.0%
!!	ATCGAATAAAATATATACTT+CGG	-	Chr3:48524161-48524180	MsG0380013963.01.T01:CDS	15.0%
!!	ATCGTAATAAATGAAAAAAA+GGG	+	Chr3:48523401-48523420	None:intergenic	15.0%
!!!	ATACAAACTTTTTTTTTTTG+AGG	+	Chr3:48524143-48524162	None:intergenic	15.0%
!!	AATCTTTCTCATTATTACAA+TGG	-	Chr3:48524115-48524134	MsG0380013963.01.T01:CDS	20.0%
!!	ACAGAATCATTTAATCATTT+CGG	-	Chr3:48523083-48523102	MsG0380013963.01.T02:CDS	20.0%
!!	ATTCATTATGTTAGATATCA+AGG	-	Chr3:48523705-48523724	MsG0380013963.01.T01:CDS	20.0%
!!	CACATACATAAAAATTATAG+AGG	+	Chr3:48523518-48523537	None:intergenic	20.0%
!!	TATTCTCAAATAATATCAGA+GGG	-	Chr3:48523153-48523172	MsG0380013963.01.T02:CDS	20.0%
!!	TCGTAATAAATGAAAAAAAG+GGG	+	Chr3:48523400-48523419	None:intergenic	20.0%
!!	TTATTCTCAAATAATATCAG+AGG	-	Chr3:48523152-48523171	MsG0380013963.01.T02:CDS	20.0%
!!	TTCCATTTATTCATCAAAAA+AGG	+	Chr3:48523759-48523778	None:intergenic	20.0%
!!!	AAAAATAGAAATCGAGTTTT+TGG	+	Chr3:48523466-48523485	None:intergenic	20.0%
!!!	ATTCTAACAAAAATCCATTT+TGG	-	Chr3:48523112-48523131	MsG0380013963.01.T02:CDS	20.0%
!!!	CGAAATTTTGTAATGTATTA+GGG	-	Chr3:48524017-48524036	MsG0380013963.01.T01:CDS	20.0%
!!!	TATTTTATTCATTTATGGCA+AGG	-	Chr3:48523783-48523802	MsG0380013963.01.T01:CDS	20.0%
!!!	TTCTAACAAAAATCCATTTT+GGG	-	Chr3:48523113-48523132	MsG0380013963.01.T02:CDS	20.0%
!!!	TTTTAATAGATCCATTTTTG+TGG	-	Chr3:48522993-48523012	MsG0380013963.01.T02:CDS	20.0%
!	AATATCAAAATCGGATGAAT+CGG	+	Chr3:48524068-48524087	None:intergenic	25.0%
!	AGGATTTATTACGTAGAAAA+AGG	+	Chr3:48523557-48523576	None:intergenic	25.0%
!	GAAATGAATGTTCGAATAAT+TGG	+	Chr3:48523851-48523870	None:intergenic	25.0%
!	GCTTTCTTGTATTAAAACTT+TGG	-	Chr3:48524183-48524202	MsG0380013963.01.T01:CDS	25.0%
!	GTAAATTTATCCTCCAAAAA+GGG	+	Chr3:48523288-48523307	None:intergenic	25.0%
!	TATCTAACATAATGAATGAG+AGG	+	Chr3:48523702-48523721	None:intergenic	25.0%
!	TCGGTCAATAATATCAAAAT+CGG	+	Chr3:48524077-48524096	None:intergenic	25.0%
!	TGTAAATTTATCCTCCAAAA+AGG	+	Chr3:48523289-48523308	None:intergenic	25.0%
!!	GCGAAATTTTGTAATGTATT+AGG	-	Chr3:48524016-48524035	MsG0380013963.01.T01:CDS	25.0%
!!	TCTAACAAAAATCCATTTTG+GGG	-	Chr3:48523114-48523133	MsG0380013963.01.T02:CDS	25.0%
!!!	AGATTTTAATCGTAAATGAG+AGG	+	Chr3:48523577-48523596	None:intergenic	25.0%
!!!	ATTTTTCGGGAGTATATTTA+TGG	-	Chr3:48522952-48522971	MsG0380013963.01.T02:CDS	25.0%
!!!	GTATACTTTAAGATTCTTCA+AGG	-	Chr3:48523674-48523693	MsG0380013963.01.T01:CDS	25.0%
!!!	TTAGGCTATTTTTAAATGTG+CGG	-	Chr3:48523876-48523895	MsG0380013963.01.T01:CDS	25.0%
	ATATACTCCCGAAAAATAAG+TGG	+	Chr3:48522949-48522968	None:intergenic	30.0%
	ATATCAAGGAAAATGCATTC+TGG	-	Chr3:48523719-48523738	MsG0380013963.01.T01:CDS	30.0%
	GATCTAATCAGAGGAATAAT+TGG	+	Chr3:48523986-48524005	None:intergenic	30.0%
	TAGAGGAACAAGAATAATCT+CGG	+	Chr3:48523501-48523520	None:intergenic	30.0%
	TATACTCCCGAAAAATAAGT+GGG	+	Chr3:48522948-48522967	None:intergenic	30.0%
	TATTCCTCTGATTAGATCAT+TGG	-	Chr3:48523988-48524007	MsG0380013963.01.T01:CDS	30.0%
	TGGTTTATATTGATCGTTCT+TGG	+	Chr3:48523831-48523850	None:intergenic	30.0%
	TTCTGCATATTCGCAAAAAT+CGG	+	Chr3:48524096-48524115	None:intergenic	30.0%
	TTCTTATTATAGCCCCAAAA+TGG	+	Chr3:48523129-48523148	None:intergenic	30.0%
	TTTGAACTAAGATTTCCAGA+TGG	+	Chr3:48523353-48523372	None:intergenic	30.0%
!	ACCTACATTTTCCACAAAAA+TGG	+	Chr3:48523007-48523026	None:intergenic	30.0%
!	CAGCTTAATTCTAGGAAAAA+TGG	+	Chr3:48523201-48523220	None:intergenic	30.0%
!	GAACATTCATTTCAGCTTTT+AGG	-	Chr3:48523858-48523877	MsG0380013963.01.T01:CDS	30.0%
!	TCTTGTAGAAGTTTTTGCTA+AGG	-	Chr3:48523644-48523663	MsG0380013963.01.T01:CDS	30.0%
!!	TCATTCCATTTTTCCCTTTT+TGG	-	Chr3:48523272-48523291	MsG0380013963.01.T01:CDS	30.0%
!!	TCCATTTTTGTGGAAAATGT+AGG	-	Chr3:48523003-48523022	MsG0380013963.01.T02:CDS	30.0%
!!	TCCTATACCCACTTATTTTT+CGG	-	Chr3:48522938-48522957	MsG0380013963.01.T02:CDS	30.0%
!!!	CGCCTTTTTTGATGAATAAA+TGG	-	Chr3:48523754-48523773	MsG0380013963.01.T01:CDS	30.0%
!!!	GGCAAGGTTTTTTTTATGTT+TGG	-	Chr3:48523799-48523818	MsG0380013963.01.T01:CDS	30.0%
!!!	TAGGCTATTTTTAAATGTGC+GGG	-	Chr3:48523877-48523896	MsG0380013963.01.T01:CDS	30.0%
	AATACCATATCCTATCCATC+TGG	-	Chr3:48523335-48523354	MsG0380013963.01.T01:CDS	35.0%
	ACTAAGATTTCCAGATGGAT+AGG	+	Chr3:48523348-48523367	None:intergenic	35.0%
	AGAATTAAGCTGTTCCTTAG+AGG	-	Chr3:48523209-48523228	MsG0380013963.01.T02:CDS	35.0%
	AGTTCAAATCCTTCGATACT+GGG	-	Chr3:48523365-48523384	MsG0380013963.01.T01:CDS	35.0%
	ATCCTCCAAAAAGGGAAAAA+TGG	+	Chr3:48523280-48523299	None:intergenic	35.0%
	ATTTCCAGATGGATAGGATA+TGG	+	Chr3:48523342-48523361	None:intergenic	35.0%
	CTAAGGAACAGCTTAATTCT+AGG	+	Chr3:48523209-48523228	None:intergenic	35.0%
	TAGTTCAAATCCTTCGATAC+TGG	-	Chr3:48523364-48523383	MsG0380013963.01.T01:CDS	35.0%
	TTAGCCAATGATCTAATCAG+AGG	+	Chr3:48523995-48524014	None:intergenic	35.0%
!!	CCTATACCCACTTATTTTTC+GGG	-	Chr3:48522939-48522958	MsG0380013963.01.T02:CDS	35.0%
!!	TTCCATTTTTCCCTTTTTGG+AGG	-	Chr3:48523275-48523294	MsG0380013963.01.T01:CDS	35.0%
	AAATGGAATTTCCACGACGA+TGG	+	Chr3:48523184-48523203	None:intergenic	40.0%
	ATTAAGCTGTTCCTTAGAGG+AGG	-	Chr3:48523212-48523231	MsG0380013963.01.T02:CDS	40.0%
	CCCGAAAAATAAGTGGGTAT+AGG	+	Chr3:48522942-48522961	None:intergenic	40.0%
	TATGATTTCTGCCTCCTCTA+AGG	+	Chr3:48523226-48523245	None:intergenic	40.0%
	ATCCTATTAGTAAGCCGGTC+TGG	-	Chr3:48524041-48524060	MsG0380013963.01.T01:CDS	45.0%
	GGGTAAATCGTTCAGTGGTA+CGG	-	Chr3:48523897-48523916	MsG0380013963.01.T01:CDS	45.0%
	TCCTATTAGTAAGCCGGTCT+GGG	-	Chr3:48524042-48524061	MsG0380013963.01.T01:CDS	45.0%
!	AGGGCATCCTATTAGTAAGC+CGG	-	Chr3:48524036-48524055	MsG0380013963.01.T01:CDS	45.0%
!	CATCTTTCACCCAGTATCGA+AGG	+	Chr3:48523377-48523396	None:intergenic	45.0%
	GTGGGTATAGGAAGTCCTGT+TGG	+	Chr3:48522930-48522949	None:intergenic	50.0%
	TGTGCGGGTAAATCGTTCAG+TGG	-	Chr3:48523892-48523911	MsG0380013963.01.T01:CDS	50.0%
	ACGAGCTAGATCTCGCCAAC+AGG	-	Chr3:48522912-48522931	MsG0380013963.01.T02:CDS	55.0%
!	AGAGGGTTTTGCCATCGTCG+TGG	-	Chr3:48523170-48523189	MsG0380013963.01.T02:CDS	55.0%
!	GCCCAGACCGGCTTACTAAT+AGG	+	Chr3:48524046-48524065	None:intergenic	55.0%
	TCGGATGAATCGGCCCAGAC+CGG	+	Chr3:48524058-48524077	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48522886	48524240	48522886	ID=MsG0380013963.01;Name=MsG0380013963.01
Chr3	mRNA	48523236	48524240	48523236	ID=MsG0380013963.01.T01;Parent=MsG0380013963.01;Name=MsG0380013963.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|334
Chr3	exon	48523236	48524240	48523236	ID=MsG0380013963.01.T01:exon:9327;Parent=MsG0380013963.01.T01
Chr3	CDS	48523236	48524240	48523236	ID=MsG0380013963.01.T01:cds;Parent=MsG0380013963.01.T01
Chr3	mRNA	48522886	48523353	48522886	ID=MsG0380013963.01.T02;Parent=MsG0380013963.01;Name=MsG0380013963.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|155
Chr3	exon	48522886	48523353	48522886	ID=MsG0380013963.01.T02:exon:9328;Parent=MsG0380013963.01.T02
Chr3	CDS	48522886	48523353	48522886	ID=MsG0380013963.01.T02:cds;Parent=MsG0380013963.01.T02
Chr3	mRNA	48522886	48523200	48522886	ID=MsG0380013963.01.T03;Parent=MsG0380013963.01;Name=MsG0380013963.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|104
Chr3	exon	48522886	48523200	48522886	ID=MsG0380013963.01.T03:exon:9329;Parent=MsG0380013963.01.T03
Chr3	CDS	48522886	48523200	48522886	ID=MsG0380013963.01.T03:cds;Parent=MsG0380013963.01.T03

Gene Sequence

>MsG0380013963.01.T02

ATGGAAACATTATTTTATTCATTTATGGCAAGGTTTTTTTTATGTTTGGTCTCAACCAAGAACGATCAATATAAACCAATTATTCGAACATTCATTTCAGCTTTTAGGCTATTTTTAAATGTGCGGGTAAATCGTTCAGTGGTACGGAGTCAAATGCTGCAAAATACATTTTTAATCGAAATTTTTAGCAAAAAACTTGATATAATAGTTCCAATTATTCCTCTGATTAGATCATTGGCTAAAGCGAAATTTTGTAATGTATTAGGGCATCCTATTAGTAAGCCGGTCTGGGCCGATTCATCCGATTTTGATATTATTGACCGATTTTTGCGAATATGCAGAAATCTTTCTCATTATTACAATGGATCCTCAAAAAAAAAAAGTTTGTATCGAATAAAATATATACTTCGGCTTTCTTGTATTAAAACTTTGGCTTGTAAACACAAAGTACTGTACGCGCTTTTTTGA

>MsG0380013963.01.T03

ATGCTGCAAAATACATTTTTAATCGAAATTTTTAGCAAAAAACTTGATATAATAGTTCCAATTATTCCTCTGATTAGATCATTGGCTAAAGCGAAATTTTGTAATGTATTAGGGCATCCTATTAGTAAGCCGGTCTGGGCCGATTCATCCGATTTTGATATTATTGACCGATTTTTGCGAATATGCAGAAATCTTTCTCATTATTACAATGGATCCTCAAAAAAAAAAAGTTTGTATCGAATAAAATATATACTTCGGCTTTCTTGTATTAAAACTTTGGCTTGTAAACACAAAGTACTGTACGCGCTTTTTTGA

>MsG0380013963.01.T01

ATGAAGGAATATCAAGTATATTTAGAACGAGCTAGATCTCGCCAACAGGACTTCCTATACCCACTTATTTTTCGGGAGTATATTTATGGACTTGCTTATAGTCATGATTTTAATAGATCCATTTTTGTGGAAAATGTAGGTTCTGACAGTAAATATAGTTTACTAATTGTAAAACGTTTAATTACTCGAATGTATCAACAGAATCATTTAATCATTTCGGCTAATGATTCTAACAAAAATCCATTTTGGGGCTATAATAAGAATTTTTATTCTCAAATAATATCAGAGGGTTTTGCCATCGTCGTGGAAATTCCATTTTTCCTAGAATTAAGCTGTTCCTTAGAGGAGGCAGAAATCATAAAATCTTATAAAAATTTGAGATCAATTCATTCCATTTTTCCCTTTTTGGAGGATAAATTTACATATTTAAATTATGTGTCAGATATACGAATACCATATCCTATCCATCTGGAAATCTTAGTTCAAATCCTTCGATACTGGGTGAAAGATGCCCCTTTTTTTCATTTATTACGATTGTTTCTTTATAATTTTAGTAATTGGAATAGTTTTATTACTACCAAAAACTCGATTTCTATTTTTTCAAAAAGTAATCCGAGATTATTCTTGTTCCTCTATAATTTTTATGTATGTGAATATGAATCTATCTTCCTTTTTCTACGTAATAAATCCTCTCATTTACGATTAAAATCTTTTAGCGTTTTTTTTGAACGAATTTTTTTTTATGCAAAAAGAGAACATCTTGTAGAAGTTTTTGCTAAGGATTTTTCGTATACTTTAAGATTCTTCAAGGATCCTCTCATTCATTATGTTAGATATCAAGGAAAATGCATTCTGGCTTCAAAGAATTCGCCTTTTTTGATGAATAAATGGAAACATTATTTTATTCATTTATGGCAAGGTTTTTTTTATGTTTGGTCTCAACCAAGAACGATCAATATAAACCAATTATTCGAACATTCATTTCAGCTTTTAGGCTATTTTTAA

Protein sequence

>MsG0380013963.01.T02

METLFYSFMARFFLCLVSTKNDQYKPIIRTFISAFRLFLNVRVNRSVVRSQMLQNTFLIEIFSKKLDIIVPIIPLIRSLAKAKFCNVLGHPISKPVWADSSDFDIIDRFLRICRNLSHYYNGSSKKKSLYRIKYILRLSCIKTLACKHKVLYALF*

>MsG0380013963.01.T03

MLQNTFLIEIFSKKLDIIVPIIPLIRSLAKAKFCNVLGHPISKPVWADSSDFDIIDRFLRICRNLSHYYNGSSKKKSLYRIKYILRLSCIKTLACKHKVLYALF*

>MsG0380013963.01.T01

MKEYQVYLERARSRQQDFLYPLIFREYIYGLAYSHDFNRSIFVENVGSDSKYSLLIVKRLITRMYQQNHLIISANDSNKNPFWGYNKNFYSQIISEGFAIVVEIPFFLELSCSLEEAEIIKSYKNLRSIHSIFPFLEDKFTYLNYVSDIRIPYPIHLEILVQILRYWVKDAPFFHLLRLFLYNFSNWNSFITTKNSISIFSKSNPRLFLFLYNFYVCEYESIFLFLRNKSSHLRLKSFSVFFERIFFYAKREHLVEVFAKDFSYTLRFFKDPLIHYVRYQGKCILASKNSPFLMNKWKHYFIHLWQGFFYVWSQPRTININQLFEHSFQLLGYF*