AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006336.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006336.01.T01 MTR_2g006290 91.503 153 10 1 1 153 1 150 9.02e-101 286
MsG0280006336.01.T01 MTR_8g088720 61.290 155 46 3 3 153 4 148 4.17e-62 188
MsG0280006336.01.T01 MTR_1g112030 55.333 150 58 3 3 151 14 155 7.08e-51 160
MsG0280006336.01.T01 MTR_1g112030 55.333 150 58 3 3 151 18 159 7.35e-51 160
MsG0280006336.01.T01 MTR_4g053120 58.519 135 42 3 28 151 23 154 1.28e-50 159
MsG0280006336.01.T01 MTR_1g112050 55.705 149 53 4 6 151 15 153 1.08e-49 157
MsG0280006336.01.T01 MTR_2g086510 61.157 121 43 2 32 151 37 154 5.93e-48 152
MsG0280006336.01.T01 MTR_5g006320 38.947 95 57 1 57 151 50 143 2.12e-17 74.3
MsG0280006336.01.T01 MTR_4g098490 39.024 82 50 0 70 151 77 158 1.33e-16 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006336.01.T01 AT1G60950 64.626 147 47 3 6 151 5 147 4.03e-61 186
MsG0280006336.01.T01 AT1G10960 63.699 146 50 2 6 151 5 147 7.72e-55 169
MsG0280006336.01.T01 AT2G27510 50.318 157 69 4 1 151 1 154 2.25e-45 146
MsG0280006336.01.T01 AT5G10000 43.662 142 75 3 14 151 7 147 1.13e-34 118
MsG0280006336.01.T01 AT4G14890 34.043 141 82 3 21 151 5 144 1.28e-18 77.8
MsG0280006336.01.T01 AT1G32550 37.037 108 68 0 44 151 46 153 5.80e-18 76.6
MsG0280006336.01.T01 AT1G32550 34.677 124 62 3 44 151 46 166 2.78e-12 62.0

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATTCTTGAGTTCCTTC+TGG 0.323416 2:+604745 None:intergenic
GAACCAGCTCTGCATGAGTA+TGG 0.373256 2:+604667 None:intergenic
ATGATCAGATTGAAGAAGGT+TGG 0.396228 2:-604576 MsG0280006336.01.T01:CDS
TTCTTCCTTGTGAGTCTCAA+TGG 0.400867 2:+604516 None:intergenic
TCAAGAATGTAAACATCATC+TGG 0.411090 2:+604718 None:intergenic
GGTTCTTGTTCTTCATGTGC+TGG 0.425033 2:-604649 MsG0280006336.01.T01:CDS
ACCGCTGGGAAAAGCCTTTG+TGG 0.440903 2:+604846 None:intergenic
GCAACCGCACCTGCTTTGTA+TGG 0.461704 2:-604937 MsG0280006336.01.T01:CDS
GTGCTGACTGACACTGGCAT+TGG 0.464692 2:+604880 None:intergenic
TGATCAGATTGAAGAAGGTT+GGG 0.468092 2:-604575 MsG0280006336.01.T01:CDS
CTTCCATACTCATGCAGAGC+TGG 0.477015 2:-604670 MsG0280006336.01.T01:CDS
CTTGACCATGCTGAGGAAGT+TGG 0.477077 2:-604700 MsG0280006336.01.T01:CDS
CATTGGCTGCCTCCTGAGGA+AGG 0.479539 2:+604897 None:intergenic
AGTTGCAATGGCTAGTTACA+AGG 0.482157 2:-604782 MsG0280006336.01.T01:CDS
CAGTGAGCACCTCCTTCCTC+AGG 0.501999 2:-604909 MsG0280006336.01.T01:CDS
AGCCTTTGTGGTTGTTGTGG+TGG 0.506920 2:+604858 None:intergenic
CTTCAATCTGATCATCATCA+AGG 0.519281 2:+604584 None:intergenic
TGTGCTGGCAAAGTTGCTGC+TGG 0.520711 2:-604634 MsG0280006336.01.T01:CDS
AAAAGCCTTTGTGGTTGTTG+TGG 0.532495 2:+604855 None:intergenic
GATGATGATCAGATTGAAGA+AGG 0.539674 2:-604580 MsG0280006336.01.T01:CDS
GTGGTGGTGCTGACTGACAC+TGG 0.553388 2:+604874 None:intergenic
CTGGCATTGGCTGCCTCCTG+AGG 0.569091 2:+604893 None:intergenic
TGGCTGCCTCCTGAGGAAGG+AGG 0.573079 2:+604900 None:intergenic
ACTGCAGTTCCATACAAAGC+AGG 0.605761 2:+604928 None:intergenic
GTGAAGCTTGTTACACCAGA+AGG 0.613290 2:-604760 MsG0280006336.01.T01:CDS
TGAGCACCTCCTTCCTCAGG+AGG 0.621811 2:-604906 MsG0280006336.01.T01:CDS
TGGTGATCTTGCAGTTGCAA+TGG 0.626955 2:-604794 MsG0280006336.01.T01:CDS
AAAAGTAACACAGGAAAGCG+TGG 0.634896 2:-604814 MsG0280006336.01.T01:CDS
TTACATTCTTGACCATGCTG+AGG 0.655914 2:-604707 MsG0280006336.01.T01:CDS
CAATACCAACTTCCTCAGCA+TGG 0.657416 2:+604695 None:intergenic
GGTGAAGTTGATCAGTCAGA+TGG 0.657495 2:-604613 MsG0280006336.01.T01:CDS
CACCACCACAACAACCACAA+AGG 0.670170 2:-604860 MsG0280006336.01.T01:CDS
AGTTCCATACAAAGCAGGTG+CGG 0.688139 2:+604933 None:intergenic
TGTTACCATTGAGACTCACA+AGG 0.695016 2:-604521 MsG0280006336.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGGTTTAAAAAGTAACAC+AGG - Chr2:604618-604637 MsG0280006336.01.T01:CDS 25.0%
TCAAGAATGTAAACATCATC+TGG + Chr2:604726-604745 None:intergenic 30.0%
ATGATCAGATTGAAGAAGGT+TGG - Chr2:604865-604884 MsG0280006336.01.T01:CDS 35.0%
CTTCAATCTGATCATCATCA+AGG + Chr2:604860-604879 None:intergenic 35.0%
GATGATGATCAGATTGAAGA+AGG - Chr2:604861-604880 MsG0280006336.01.T01:CDS 35.0%
TCAAATTCTTGAGTTCCTTC+TGG + Chr2:604699-604718 None:intergenic 35.0%
TGATCAGATTGAAGAAGGTT+GGG - Chr2:604866-604885 MsG0280006336.01.T01:CDS 35.0%
! ACTTTTTAAACCAAAACCGC+TGG + Chr2:604613-604632 None:intergenic 35.0%
! CTTTTTAAACCAAAACCGCT+GGG + Chr2:604612-604631 None:intergenic 35.0%
AAAAGCCTTTGTGGTTGTTG+TGG + Chr2:604589-604608 None:intergenic 40.0%
AAAAGTAACACAGGAAAGCG+TGG - Chr2:604627-604646 MsG0280006336.01.T01:CDS 40.0%
TGTTACCATTGAGACTCACA+AGG - Chr2:604920-604939 MsG0280006336.01.T01:CDS 40.0%
TTACATTCTTGACCATGCTG+AGG - Chr2:604734-604753 MsG0280006336.01.T01:CDS 40.0%
TTCTTCCTTGTGAGTCTCAA+TGG + Chr2:604928-604947 None:intergenic 40.0%
! AGTTGCAATGGCTAGTTACA+AGG - Chr2:604659-604678 MsG0280006336.01.T01:CDS 40.0%
ACTGCAGTTCCATACAAAGC+AGG + Chr2:604516-604535 None:intergenic 45.0%
AGTTCCATACAAAGCAGGTG+CGG + Chr2:604511-604530 None:intergenic 45.0%
CAATACCAACTTCCTCAGCA+TGG + Chr2:604749-604768 None:intergenic 45.0%
GGTTCTTGTTCTTCATGTGC+TGG - Chr2:604792-604811 MsG0280006336.01.T01:CDS 45.0%
TGGTGATCTTGCAGTTGCAA+TGG - Chr2:604647-604666 MsG0280006336.01.T01:CDS 45.0%
! GGTGAAGTTGATCAGTCAGA+TGG - Chr2:604828-604847 MsG0280006336.01.T01:CDS 45.0%
!! GTGAAGCTTGTTACACCAGA+AGG - Chr2:604681-604700 MsG0280006336.01.T01:CDS 45.0%
AGCCTTTGTGGTTGTTGTGG+TGG + Chr2:604586-604605 None:intergenic 50.0%
CACCACCACAACAACCACAA+AGG - Chr2:604581-604600 MsG0280006336.01.T01:CDS 50.0%
CTTCCATACTCATGCAGAGC+TGG - Chr2:604771-604790 MsG0280006336.01.T01:CDS 50.0%
CTTGACCATGCTGAGGAAGT+TGG - Chr2:604741-604760 MsG0280006336.01.T01:CDS 50.0%
GAACCAGCTCTGCATGAGTA+TGG + Chr2:604777-604796 None:intergenic 50.0%
! AAGGCTTTTCCCAGCGGTTT+TGG - Chr2:604600-604619 MsG0280006336.01.T01:CDS 50.0%
! ACCACAAAGGCTTTTCCCAG+CGG - Chr2:604594-604613 MsG0280006336.01.T01:CDS 50.0%
ACCGCTGGGAAAAGCCTTTG+TGG + Chr2:604598-604617 None:intergenic 55.0%
GCAACCGCACCTGCTTTGTA+TGG - Chr2:604504-604523 MsG0280006336.01.T01:CDS 55.0%
! TGTGCTGGCAAAGTTGCTGC+TGG - Chr2:604807-604826 MsG0280006336.01.T01:CDS 55.0%
!! GTGCTGACTGACACTGGCAT+TGG + Chr2:604564-604583 None:intergenic 55.0%
CAGTGAGCACCTCCTTCCTC+AGG - Chr2:604532-604551 MsG0280006336.01.T01:CDS 60.0%
TGAGCACCTCCTTCCTCAGG+AGG - Chr2:604535-604554 MsG0280006336.01.T01:CDS 60.0%
! CATTGGCTGCCTCCTGAGGA+AGG + Chr2:604547-604566 None:intergenic 60.0%
!! GTGGTGGTGCTGACTGACAC+TGG + Chr2:604570-604589 None:intergenic 60.0%
TGGCTGCCTCCTGAGGAAGG+AGG + Chr2:604544-604563 None:intergenic 65.0%
!! CTGGCATTGGCTGCCTCCTG+AGG + Chr2:604551-604570 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 604501 604962 604501 ID=MsG0280006336.01;Name=MsG0280006336.01
Chr2 mRNA 604501 604962 604501 ID=MsG0280006336.01.T01;Parent=MsG0280006336.01;Name=MsG0280006336.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|153
Chr2 exon 604501 604962 604501 ID=MsG0280006336.01.T01:exon:771;Parent=MsG0280006336.01.T01
Chr2 CDS 604501 604962 604501 ID=MsG0280006336.01.T01:cds;Parent=MsG0280006336.01.T01
Gene Sequence

>MsG0280006336.01.T01

ATGGCAACCGCACCTGCTTTGTATGGAACTGCAGTGAGCACCTCCTTCCTCAGGAGGCAGCCAATGCCAGTGTCAGTCAGCACCACCACAACAACCACAAAGGCTTTTCCCAGCGGTTTTGGTTTAAAAAGTAACACAGGAAAGCGTGGTGATCTTGCAGTTGCAATGGCTAGTTACAAGGTGAAGCTTGTTACACCAGAAGGAACTCAAGAATTTGAATGTCCAGATGATGTTTACATTCTTGACCATGCTGAGGAAGTTGGTATTGAACTTCCATACTCATGCAGAGCTGGTTCTTGTTCTTCATGTGCTGGCAAAGTTGCTGCTGGTGAAGTTGATCAGTCAGATGGTTCTTTCCTTGATGATGATCAGATTGAAGAAGGTTGGGTTCTTACATGTGTTGCTTATGCTAAGTCTGATGTTACCATTGAGACTCACAAGGAAGAAGAACTCACTGCTTAA

Protein sequence

>MsG0280006336.01.T01

MATAPALYGTAVSTSFLRRQPMPVSVSTTTTTTKAFPSGFGLKSNTGKRGDLAVAMASYKVKLVTPEGTQEFECPDDVYILDHAEEVGIELPYSCRAGSCSSCAGKVAAGEVDQSDGSFLDDDQIEEGWVLTCVAYAKSDVTIETHKEEELTA*