Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013976.01.T01 | YP_003587580.1 | 95.833 | 48 | 2 | 0 | 1 | 48 | 1 | 48 | 6.08E-25 | 97.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013976.01.T01 | A0A0F6NG12 | 95.833 | 48 | 2 | 0 | 1 | 48 | 1 | 48 | 2.90e-25 | 97.4 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048059.01 | MsG0380013976.01 | 0.833766 | 4.416210e-56 | 5.136231e-53 |
| MsG0080048560.01 | MsG0380013976.01 | 0.854654 | 1.065693e-61 | 2.397195e-58 |
| MsG0080048611.01 | MsG0380013976.01 | 0.800060 | 1.720688e-48 | 7.935460e-46 |
| MsG0080048689.01 | MsG0380013976.01 | 0.840879 | 6.664874e-58 | 9.624664e-55 |
| MsG0080048690.01 | MsG0380013976.01 | 0.823518 | 1.326417e-53 | 1.144696e-50 |
| MsG0080048691.01 | MsG0380013976.01 | 0.842468 | 2.539194e-58 | 3.853888e-55 |
| MsG0080048697.01 | MsG0380013976.01 | 0.836090 | 1.146972e-56 | 1.430475e-53 |
| MsG0080048896.01 | MsG0380013976.01 | 0.846885 | 1.639139e-59 | 2.862031e-56 |
| MsG0080048909.01 | MsG0380013976.01 | 0.816761 | 4.682234e-52 | 3.347212e-49 |
| MsG0080048929.01 | MsG0380013976.01 | 0.807465 | 4.989465e-50 | 2.781079e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013976.01.T01 | MTR_4g051375 | 93.750 | 48 | 3 | 0 | 1 | 48 | 1 | 48 | 1.91e-27 | 94.4 |
| MsG0380013976.01.T01 | MTR_2g062930 | 97.826 | 46 | 1 | 0 | 1 | 46 | 1 | 46 | 4.19e-27 | 94.4 |
Find 21 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCGTTGAATTCAGAATA+TGG | 0.285183 | 3:+48550450 | MsG0380013976.01.T01:CDS |
| AAAGGGGTAGTTCCCCATCC+CGG | 0.593845 | 3:-48550483 | None:intergenic |
| AAGTTGCAATACCCATCAAA+GGG | 0.519804 | 3:-48550500 | None:intergenic |
| AGAATATGGTAAAGTAGCTC+CGG | 0.467064 | 3:+48550464 | MsG0380013976.01.T01:CDS |
| AGATCTAGAACTACCCTCAA+CGG | 0.642132 | 3:-48550394 | None:intergenic |
| AGCCAAGGCTTAGCTCAGGT+CGG | 0.612646 | 3:-48550526 | None:intergenic |
| AGTTGCAATACCCATCAAAG+GGG | 0.733608 | 3:-48550499 | None:intergenic |
| ATGGCTACACAAACCGTTGA+GGG | 0.512439 | 3:+48550381 | MsG0380013976.01.T01:CDS |
| ATGGGGAACTACCCCTTTGA+TGG | 0.408879 | 3:+48550488 | MsG0380013976.01.T01:CDS |
| ATGGTAAAGTAGCTCCGGGA+TGG | 0.560142 | 3:+48550469 | MsG0380013976.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCGTTGAATTCAGAATA+TGG | + | Chr3:48550450-48550469 | MsG0380013976.01.T01:CDS | 30.0% | |
| AAGTTGCAATACCCATCAAA+GGG | - | Chr3:48550503-48550522 | None:intergenic | 35.0% | |
| AGAATATGGTAAAGTAGCTC+CGG | + | Chr3:48550464-48550483 | MsG0380013976.01.T01:CDS | 35.0% | |
| AGTTGCAATACCCATCAAAG+GGG | - | Chr3:48550502-48550521 | None:intergenic | 40.0% | |
| CAAGTTGCAATACCCATCAA+AGG | - | Chr3:48550504-48550523 | None:intergenic | 40.0% | |
| GAATATGGTAAAGTAGCTCC+GGG | + | Chr3:48550465-48550484 | MsG0380013976.01.T01:CDS | 40.0% | |
| ATGGCTACACAAACCGTTGA+GGG | + | Chr3:48550381-48550400 | MsG0380013976.01.T01:CDS | 45.0% | |
| TCTCCTACAACAGTTCGTCT+TGG | - | Chr3:48550423-48550442 | None:intergenic | 45.0% | |
| ATGGGGAACTACCCCTTTGA+TGG | + | Chr3:48550488-48550507 | MsG0380013976.01.T01:CDS | 50.0% | |
| ATGGTAAAGTAGCTCCGGGA+TGG | + | Chr3:48550469-48550488 | MsG0380013976.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48550381 | 48550539 | 48550381 | ID=MsG0380013976.01;Name=MsG0380013976.01 |
| Chr3 | mRNA | 48550381 | 48550539 | 48550381 | ID=MsG0380013976.01.T01;Parent=MsG0380013976.01;Name=MsG0380013976.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|52 |
| Chr3 | exon | 48550381 | 48550539 | 48550381 | ID=MsG0380013976.01.T01:exon:8589;Parent=MsG0380013976.01.T01 |
| Chr3 | CDS | 48550381 | 48550539 | 48550381 | ID=MsG0380013976.01.T01:cds;Parent=MsG0380013976.01.T01 |
| Gene Sequence |
>MsG0380013976.01.T01 ATGGCTACACAAACCGTTGAGGGTAGTTCTAGATCTGGTCCAAGACGAACTGTTGTAGGAGATTTATTGAAACCGTTGAATTCAGAATATGGTAAAGTAGCTCCGGGATGGGGAACTACCCCTTTGATGGGTATTGCAACTTGTTCCGACCTGAGCTAA |
| Protein sequence |
>MsG0380013976.01.T01 MATQTVEGSSRSGPRRTVVGDLLKPLNSEYGKVAPGWGTTPLMGIATCSDLS* |
