AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013976.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013976.01.T01 MTR_4g051375 93.750 48 3 0 1 48 1 48 1.91e-27 94.4
MsG0380013976.01.T01 MTR_2g062930 97.826 46 1 0 1 46 1 46 4.19e-27 94.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013976.01.T01 ATCG00710 89.130 46 5 0 1 46 1 46 1.11e-24 86.7

Find 21 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCGTTGAATTCAGAATA+TGG 0.285183 3:+48550450 MsG0380013976.01.T01:CDS
ATGGGGAACTACCCCTTTGA+TGG 0.408879 3:+48550488 MsG0380013976.01.T01:CDS
GGTCCAAGACGAACTGTTGT+AGG 0.449268 3:+48550417 MsG0380013976.01.T01:CDS
AGAATATGGTAAAGTAGCTC+CGG 0.467064 3:+48550464 MsG0380013976.01.T01:CDS
ATGGCTACACAAACCGTTGA+GGG 0.512439 3:+48550381 MsG0380013976.01.T01:CDS
AAGTTGCAATACCCATCAAA+GGG 0.519804 3:-48550500 None:intergenic
GTTGAGGGTAGTTCTAGATC+TGG 0.522119 3:+48550396 MsG0380013976.01.T01:CDS
TGGTAAAGTAGCTCCGGGAT+GGG 0.527741 3:+48550470 MsG0380013976.01.T01:CDS
TCTCCTACAACAGTTCGTCT+TGG 0.532181 3:-48550420 None:intergenic
GGTAAAGTAGCTCCGGGATG+GGG 0.547587 3:+48550471 MsG0380013976.01.T01:CDS
TTACCATATTCTGAATTCAA+CGG 0.547816 3:-48550453 None:intergenic
GTTTAGCCAAGGCTTAGCTC+AGG 0.551669 3:-48550530 None:intergenic
TGGGGAACTACCCCTTTGAT+GGG 0.558023 3:+48550489 MsG0380013976.01.T01:CDS
ATGGTAAAGTAGCTCCGGGA+TGG 0.560142 3:+48550469 MsG0380013976.01.T01:CDS
CAAGTTGCAATACCCATCAA+AGG 0.570943 3:-48550501 None:intergenic
GAATATGGTAAAGTAGCTCC+GGG 0.573000 3:+48550465 MsG0380013976.01.T01:CDS
AAAGGGGTAGTTCCCCATCC+CGG 0.593845 3:-48550483 None:intergenic
AGCCAAGGCTTAGCTCAGGT+CGG 0.612646 3:-48550526 None:intergenic
TATGGCTACACAAACCGTTG+AGG 0.620476 3:+48550380 None:intergenic
AGATCTAGAACTACCCTCAA+CGG 0.642132 3:-48550394 None:intergenic
AGTTGCAATACCCATCAAAG+GGG 0.733608 3:-48550499 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTACCATATTCTGAATTCAA+CGG - Chr3:48550456-48550475 None:intergenic 25.0%
AAACCGTTGAATTCAGAATA+TGG + Chr3:48550450-48550469 MsG0380013976.01.T01:CDS 30.0%
AAGTTGCAATACCCATCAAA+GGG - Chr3:48550503-48550522 None:intergenic 35.0%
AGAATATGGTAAAGTAGCTC+CGG + Chr3:48550464-48550483 MsG0380013976.01.T01:CDS 35.0%
AGTTGCAATACCCATCAAAG+GGG - Chr3:48550502-48550521 None:intergenic 40.0%
CAAGTTGCAATACCCATCAA+AGG - Chr3:48550504-48550523 None:intergenic 40.0%
GAATATGGTAAAGTAGCTCC+GGG + Chr3:48550465-48550484 MsG0380013976.01.T01:CDS 40.0%
! AGATCTAGAACTACCCTCAA+CGG - Chr3:48550397-48550416 None:intergenic 40.0%
ATGGCTACACAAACCGTTGA+GGG + Chr3:48550381-48550400 MsG0380013976.01.T01:CDS 45.0%
TCTCCTACAACAGTTCGTCT+TGG - Chr3:48550423-48550442 None:intergenic 45.0%
!! GTTGAGGGTAGTTCTAGATC+TGG + Chr3:48550396-48550415 MsG0380013976.01.T01:CDS 45.0%
ATGGGGAACTACCCCTTTGA+TGG + Chr3:48550488-48550507 MsG0380013976.01.T01:CDS 50.0%
ATGGTAAAGTAGCTCCGGGA+TGG + Chr3:48550469-48550488 MsG0380013976.01.T01:CDS 50.0%
GGTCCAAGACGAACTGTTGT+AGG + Chr3:48550417-48550436 MsG0380013976.01.T01:CDS 50.0%
TGGGGAACTACCCCTTTGAT+GGG + Chr3:48550489-48550508 MsG0380013976.01.T01:CDS 50.0%
TGGTAAAGTAGCTCCGGGAT+GGG + Chr3:48550470-48550489 MsG0380013976.01.T01:CDS 50.0%
AAAGGGGTAGTTCCCCATCC+CGG - Chr3:48550486-48550505 None:intergenic 55.0%
GGTAAAGTAGCTCCGGGATG+GGG + Chr3:48550471-48550490 MsG0380013976.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48550381 48550539 48550381 ID=MsG0380013976.01;Name=MsG0380013976.01
Chr3 mRNA 48550381 48550539 48550381 ID=MsG0380013976.01.T01;Parent=MsG0380013976.01;Name=MsG0380013976.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|52
Chr3 exon 48550381 48550539 48550381 ID=MsG0380013976.01.T01:exon:8589;Parent=MsG0380013976.01.T01
Chr3 CDS 48550381 48550539 48550381 ID=MsG0380013976.01.T01:cds;Parent=MsG0380013976.01.T01
Gene Sequence

>MsG0380013976.01.T01

ATGGCTACACAAACCGTTGAGGGTAGTTCTAGATCTGGTCCAAGACGAACTGTTGTAGGAGATTTATTGAAACCGTTGAATTCAGAATATGGTAAAGTAGCTCCGGGATGGGGAACTACCCCTTTGATGGGTATTGCAACTTGTTCCGACCTGAGCTAA

Protein sequence

>MsG0380013976.01.T01

MATQTVEGSSRSGPRRTVVGDLLKPLNSEYGKVAPGWGTTPLMGIATCSDLS*