AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013579.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013579.01.T01 MTR_3g046760 72.115 104 7 1 1 82 52 155 6.61e-34 116
MsG0380013579.01.T01 MTR_4g096780 45.918 98 30 1 8 82 54 151 5.70e-21 83.6
MsG0380013579.01.T01 MTR_4g096780 45.918 98 30 1 8 82 54 151 9.83e-21 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013579.01.T01 AT2G30890 42.857 105 37 1 1 82 45 149 3.55e-24 91.7
MsG0380013579.01.T01 AT4G18260 45.918 98 30 1 8 82 60 157 1.01e-22 88.6

Find 20 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTAGGGGTTGCACTTTA+TGG 0.214906 3:+41090635 MsG0380013579.01.T01:CDS
CTTTCTCCTTTGGGCTTCAA+TGG 0.240506 3:+41089471 MsG0380013579.01.T01:CDS
ACAATAATCATCAAAGATTA+GGG 0.240819 3:+41090621 MsG0380013579.01.T01:CDS
CATTACATGGCTTTCTCCTT+TGG 0.245393 3:+41089461 MsG0380013579.01.T01:CDS
TTGCACTTTATGGTGTTATC+TGG 0.283526 3:+41090645 MsG0380013579.01.T01:CDS
ATTACATGGCTTTCTCCTTT+GGG 0.302177 3:+41089462 MsG0380013579.01.T01:CDS
AACAATAATCATCAAAGATT+AGG 0.323426 3:+41090620 MsG0380013579.01.T01:CDS
ATGGGATTCTTGATGCCTAT+TGG 0.346223 3:+41089490 MsG0380013579.01.T01:CDS
GTAATTTCAAATTGAAGTCT+TGG 0.346934 3:-41089439 None:intergenic
CAATTTGAAATTACATTACA+TGG 0.377562 3:+41089448 MsG0380013579.01.T01:CDS
TTTCTCCTTTGGGCTTCAAT+GGG 0.382298 3:+41089472 MsG0380013579.01.T01:CDS
GCTGTACTTCTTGCCACAGC+AGG 0.491683 3:+41090560 MsG0380013579.01.T01:CDS
TTTAGAGTTTCTGTTAAAGC+AGG 0.511706 3:+41090532 MsG0380013579.01.T01:intron
ATCTGGCTGCAAGTCCTAGT+TGG 0.526574 3:+41090662 MsG0380013579.01.T01:CDS
TTTGATGATTATTGTTGAAG+AGG 0.545214 3:-41090613 None:intergenic
TGACATGATTGCTCCTGCTG+TGG 0.597152 3:-41090573 None:intergenic
AGAATCCCATTGAAGCCCAA+AGG 0.642048 3:-41089477 None:intergenic
TTGGTATCTTTCGACCACGA+AGG 0.667973 3:+41090681 MsG0380013579.01.T01:CDS
GTGGTCGAAAGATACCAACT+AGG 0.688430 3:-41090676 None:intergenic
CAATAATCATCAAAGATTAG+GGG 0.720596 3:+41090622 MsG0380013579.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATGTTTTAAAAAAAGT+CGG - Chr3:41090474-41090493 None:intergenic 10.0%
!! GAATAAATTAACTAAAAATG+TGG - Chr3:41090152-41090171 None:intergenic 15.0%
!!! TAATGCATAAGTTTTAAAAA+TGG + Chr3:41089830-41089849 MsG0380013579.01.T01:intron 15.0%
!!! TATTCATTCTATTACTTTTA+AGG + Chr3:41089874-41089893 MsG0380013579.01.T01:intron 15.0%
!! AACAATAATCATCAAAGATT+AGG + Chr3:41090620-41090639 MsG0380013579.01.T01:CDS 20.0%
!! AATTAGATTATCAAATAGAG+AGG + Chr3:41089522-41089541 MsG0380013579.01.T01:intron 20.0%
!! AATTATAGTCTTTGTCAAAA+AGG - Chr3:41090223-41090242 None:intergenic 20.0%
!! ACAATAATCATCAAAGATTA+GGG + Chr3:41090621-41090640 MsG0380013579.01.T01:CDS 20.0%
!! CTAATAAGATCATGTAATAT+TGG + Chr3:41090188-41090207 MsG0380013579.01.T01:intron 20.0%
!! TACATAAATAGGTCAATTAA+AGG - Chr3:41089977-41089996 None:intergenic 20.0%
!!! CAATTTGAAATTACATTACA+TGG + Chr3:41089448-41089467 MsG0380013579.01.T01:CDS 20.0%
!!! TCTTTTTGATGATAATATGA+AGG + Chr3:41090283-41090302 MsG0380013579.01.T01:intron 20.0%
! AAAATATTGACAGATCCAAA+TGG - Chr3:41090042-41090061 None:intergenic 25.0%
! AAATATTGACAGATCCAAAT+GGG - Chr3:41090041-41090060 None:intergenic 25.0%
! AGACTATAATTGATACTTTG+AGG + Chr3:41090232-41090251 MsG0380013579.01.T01:intron 25.0%
! CAATAATCATCAAAGATTAG+GGG + Chr3:41090622-41090641 MsG0380013579.01.T01:CDS 25.0%
! CTATTTATGTAATAAAGCAG+TGG + Chr3:41089986-41090005 MsG0380013579.01.T01:intron 25.0%
! GTAATTTCAAATTGAAGTCT+TGG - Chr3:41089442-41089461 None:intergenic 25.0%
!! CACTGCTTTATTACATAAAT+AGG - Chr3:41089988-41090007 None:intergenic 25.0%
!! CTATGTTCATACAATTTTTC+AGG + Chr3:41089573-41089592 MsG0380013579.01.T01:intron 25.0%
!! TTTGATGATTATTGTTGAAG+AGG - Chr3:41090616-41090635 None:intergenic 25.0%
!!! GAATTTATTTTGTATTCTGC+TGG + Chr3:41089647-41089666 MsG0380013579.01.T01:intron 25.0%
AATAAAATTCTAAGCCACCT+TGG - Chr3:41089553-41089572 None:intergenic 30.0%
AATATTGACAGATCCAAATG+GGG - Chr3:41090040-41090059 None:intergenic 30.0%
ATAAAGATCAGTTGTCACAT+TGG + Chr3:41090072-41090091 MsG0380013579.01.T01:intron 30.0%
CTAATTGCAAGTATACCAAT+AGG - Chr3:41089508-41089527 None:intergenic 30.0%
TGACACGATACTAACATATA+TGG - Chr3:41090398-41090417 None:intergenic 30.0%
! CATTGGTTAACCTTTAATGA+TGG + Chr3:41090089-41090108 MsG0380013579.01.T01:intron 30.0%
! TTTAGAGTTTCTGTTAAAGC+AGG + Chr3:41090532-41090551 MsG0380013579.01.T01:intron 30.0%
ACCTTTAATGATGGCATGTA+AGG + Chr3:41090098-41090117 MsG0380013579.01.T01:intron 35.0%
ATTACATGGCTTTCTCCTTT+GGG + Chr3:41089462-41089481 MsG0380013579.01.T01:CDS 35.0%
GACCACAGAATATCTATCTA+AGG - Chr3:41089945-41089964 None:intergenic 35.0%
TCCTTACATGCCATCATTAA+AGG - Chr3:41090102-41090121 None:intergenic 35.0%
TGCCTTAGATAGATATTCTG+TGG + Chr3:41089940-41089959 MsG0380013579.01.T01:intron 35.0%
! TAAAGCAGTGGTGAAAGTTT+TGG + Chr3:41089998-41090017 MsG0380013579.01.T01:intron 35.0%
!! AATTTTCTTGCTGTCTTCAC+AGG - Chr3:41090337-41090356 None:intergenic 35.0%
!! TTGCACTTTATGGTGTTATC+TGG + Chr3:41090645-41090664 MsG0380013579.01.T01:CDS 35.0%
!!! TGGCTGCTTTTCTTTTTACT+TGG + Chr3:41089667-41089686 MsG0380013579.01.T01:intron 35.0%
AGATTAGGGGTTGCACTTTA+TGG + Chr3:41090635-41090654 MsG0380013579.01.T01:CDS 40.0%
ATGGGATTCTTGATGCCTAT+TGG + Chr3:41089490-41089509 MsG0380013579.01.T01:CDS 40.0%
CAAATAGAGAGGAGAATCCA+AGG + Chr3:41089533-41089552 MsG0380013579.01.T01:intron 40.0%
CATTACATGGCTTTCTCCTT+TGG + Chr3:41089461-41089480 MsG0380013579.01.T01:CDS 40.0%
TTTCTCCTTTGGGCTTCAAT+GGG + Chr3:41089472-41089491 MsG0380013579.01.T01:CDS 40.0%
!! GTGGTGAAAGTTTTGGTAGA+TGG + Chr3:41090005-41090024 MsG0380013579.01.T01:intron 40.0%
!! GTTTTGGTAGATGGTTACTC+TGG + Chr3:41090014-41090033 MsG0380013579.01.T01:intron 40.0%
AGAATCCCATTGAAGCCCAA+AGG - Chr3:41089480-41089499 None:intergenic 45.0%
ATAGAGAGGAGAATCCAAGG+TGG + Chr3:41089536-41089555 MsG0380013579.01.T01:intron 45.0%
CTTTCTCCTTTGGGCTTCAA+TGG + Chr3:41089471-41089490 MsG0380013579.01.T01:CDS 45.0%
GTGGTCGAAAGATACCAACT+AGG - Chr3:41090679-41090698 None:intergenic 45.0%
TTGGTATCTTTCGACCACGA+AGG + Chr3:41090681-41090700 MsG0380013579.01.T01:CDS 45.0%
!! CTTAAATAATTAAATATAAT+TGG - Chr3:41089696-41089715 None:intergenic 5.0%
!!! AATTAGTAATTATTTAAAAT+AGG + Chr3:41089738-41089757 MsG0380013579.01.T01:intron 5.0%
ATCTGGCTGCAAGTCCTAGT+TGG + Chr3:41090662-41090681 MsG0380013579.01.T01:CDS 50.0%
ATGGTTACTCTGGCCCCATT+TGG + Chr3:41090024-41090043 MsG0380013579.01.T01:intron 50.0%
TGACATGATTGCTCCTGCTG+TGG - Chr3:41090576-41090595 None:intergenic 50.0%
GCTGTACTTCTTGCCACAGC+AGG + Chr3:41090560-41090579 MsG0380013579.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 41089433 41090706 41089433 ID=MsG0380013579.01;Name=MsG0380013579.01
Chr3 mRNA 41089433 41090706 41089433 ID=MsG0380013579.01.T01;Parent=MsG0380013579.01;Name=MsG0380013579.01.T01;_AED=0.49;_eAED=0.52;_QI=0|0|0|1|0|0|2|0|82
Chr3 exon 41089433 41089511 41089433 ID=MsG0380013579.01.T01:exon:14655;Parent=MsG0380013579.01.T01
Chr3 exon 41090537 41090706 41090537 ID=MsG0380013579.01.T01:exon:14656;Parent=MsG0380013579.01.T01
Chr3 CDS 41089433 41089511 41089433 ID=MsG0380013579.01.T01:cds;Parent=MsG0380013579.01.T01
Chr3 CDS 41090537 41090706 41090537 ID=MsG0380013579.01.T01:cds;Parent=MsG0380013579.01.T01
Gene Sequence

>MsG0380013579.01.T01

ATGAGTCCAAGACTTCAATTTGAAATTACATTACATGGCTTTCTCCTTTGGGCTTCAATGGGATTCTTGATGCCTATTGAGTTTCTGTTAAAGCAGGTAGTTGCTGTACTTCTTGCCACAGCAGGAGCAATCATGTCAATAAAAAATTTCAACAACCTCTTCAACAATAATCATCAAAGATTAGGGGTTGCACTTTATGGTGTTATCTGGCTGCAAGTCCTAGTTGGTATCTTTCGACCACGAAGGTAA

Protein sequence

>MsG0380013579.01.T01

MSPRLQFEITLHGFLLWASMGFLMPIEFLLKQVVAVLLATAGAIMSIKNFNNLFNNNHQRLGVALYGVIWLQVLVGIFRPRR*