AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017797.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAATTTGTTCCAGGATTT+TGG 0.134941 3:-100752492 MsG0380017797.01.T01:intron
TGTTTCTCCTTCTCTTGTTT+TGG 0.171794 3:-100752836 MsG0380017797.01.T01:CDS
TACAAATAATTACCATAAAA+TGG 0.225925 3:+100752374 None:intergenic
TACTTCCACGTGAAGGTTGC+TGG 0.335178 3:-100752889 MsG0380017797.01.T01:five_prime_UTR
AAAGTTTCAGCGCTTCCTTT+TGG 0.350105 3:+100753072 None:intergenic
TTAATTGAATAGAATGTAGA+TGG 0.357479 3:-100752919 MsG0380017797.01.T01:intron
CTTTCCAGAATTTAACTTTG+CGG 0.419957 3:+100753046 None:intergenic
TGTGCACTCTCAATCACAAT+TGG 0.466118 3:+100752417 None:intergenic
ATAAAATGGAAGCGGGGCCA+AGG 0.466360 3:+100752388 None:intergenic
TAAAATGGAAGCGGGGCCAA+GGG 0.471744 3:+100752389 None:intergenic
AATTACCATAAAATGGAAGC+GGG 0.497706 3:+100752381 None:intergenic
ACTTCCACGTGAAGGTTGCT+GGG 0.535786 3:-100752888 MsG0380017797.01.T01:five_prime_UTR
AGATCGTGGTGAAGTCCAAA+AGG 0.544645 3:-100753087 MsG0380017797.01.T01:five_prime_UTR
ACAATGCGTTGAGTTGAAGA+AGG 0.555886 3:-100753298 MsG0380017797.01.T01:five_prime_UTR
TTCTGGAAAGAAGACGCTGA+TGG 0.556197 3:-100753033 MsG0380017797.01.T01:five_prime_UTR
TAATTACCATAAAATGGAAG+CGG 0.561078 3:+100752380 None:intergenic
GAGAGTGCACAGATGCCCCT+TGG 0.565824 3:-100752405 MsG0380017797.01.T01:three_prime_UTR
AAAAGTTCCAAAACAAGAGA+AGG 0.567363 3:+100752829 None:intergenic
AAAATGGAAGCGGGGCCAAG+GGG 0.595531 3:+100752390 None:intergenic
CGTGTCATCAAAGATTACCT+TGG 0.619516 3:-100752793 MsG0380017797.01.T01:CDS
CATCAAGAAGCTCATAGACA+AGG 0.620398 3:+100752734 None:intergenic
TGTGCAAACAACATCTACTG+AGG 0.675679 3:-100752466 MsG0380017797.01.T01:CDS
ATAACCCAGCAACCTTCACG+TGG 0.682802 3:+100752884 None:intergenic
ATTACCATAAAATGGAAGCG+GGG 0.703729 3:+100752382 None:intergenic
TGTGAACTACTTCCACGTGA+AGG 0.725411 3:-100752896 MsG0380017797.01.T01:five_prime_UTR
AGTCTTCATTAAGAACACCA+AGG 0.729590 3:+100752776 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTGAATTTCATGTTAT+CGG + Chr3:100752570-100752589 None:intergenic 15.0%
!! AAAAAAAACTACAAAAACAG+TGG + Chr3:100752685-100752704 None:intergenic 20.0%
!! ATTGTAAGTACTTATTAACT+TGG - Chr3:100752979-100752998 MsG0380017797.01.T01:intron 20.0%
!! TTAATTGAATAGAATGTAGA+TGG - Chr3:100752766-100752785 MsG0380017797.01.T01:CDS 20.0%
! AAATAAATAAATGCAGATCG+TGG - Chr3:100752584-100752603 MsG0380017797.01.T01:intron 25.0%
AAAAGTTCCAAAACAAGAGA+AGG + Chr3:100752859-100752878 None:intergenic 30.0%
! CTTTCCAGAATTTAACTTTG+CGG + Chr3:100752642-100752661 None:intergenic 30.0%
! TTTTCCGCAAAGTTAAATTC+TGG - Chr3:100752635-100752654 MsG0380017797.01.T01:intron 30.0%
AGTCTTCATTAAGAACACCA+AGG + Chr3:100752912-100752931 None:intergenic 35.0%
TGTATCTTGCTGCACTAAAT+GGG + Chr3:100753042-100753061 None:intergenic 35.0%
TTTGCACATAACCAAAATCC+TGG + Chr3:100753207-100753226 None:intergenic 35.0%
! AGTTCTTTTGCTTTCTCAAG+AGG - Chr3:100752447-100752466 MsG0380017797.01.T01:exon 35.0%
!! GTCAATTTGTTCCAGGATTT+TGG - Chr3:100753193-100753212 MsG0380017797.01.T01:intron 35.0%
!! TGTTTCTCCTTCTCTTGTTT+TGG - Chr3:100752849-100752868 MsG0380017797.01.T01:exon 35.0%
CATCAAGAAGCTCATAGACA+AGG + Chr3:100752954-100752973 None:intergenic 40.0%
CGTGTCATCAAAGATTACCT+TGG - Chr3:100752892-100752911 MsG0380017797.01.T01:five_prime_UTR 40.0%
GTGTATCTTGCTGCACTAAA+TGG + Chr3:100753043-100753062 None:intergenic 40.0%
TGTGCAAACAACATCTACTG+AGG - Chr3:100753219-100753238 MsG0380017797.01.T01:five_prime_UTR 40.0%
TGTGCACTCTCAATCACAAT+TGG + Chr3:100753271-100753290 None:intergenic 40.0%
! AAAGTTTCAGCGCTTCCTTT+TGG + Chr3:100752616-100752635 None:intergenic 40.0%
! TGCTGAAGTCAATTTGTTCC+AGG - Chr3:100753186-100753205 MsG0380017797.01.T01:intron 40.0%
!! ACAATGCGTTGAGTTGAAGA+AGG - Chr3:100752387-100752406 MsG0380017797.01.T01:three_prime_UTR 40.0%
AGATCGTGGTGAAGTCCAAA+AGG - Chr3:100752598-100752617 MsG0380017797.01.T01:intron 45.0%
GAGTAGTTGCTATAGCGTAG+CGG - Chr3:100753089-100753108 MsG0380017797.01.T01:intron 45.0%
TGTGAACTACTTCCACGTGA+AGG - Chr3:100752789-100752808 MsG0380017797.01.T01:CDS 45.0%
! TTCTGGAAAGAAGACGCTGA+TGG - Chr3:100752652-100752671 MsG0380017797.01.T01:intron 45.0%
!! ATTATTTATAAAAAAGAAAT+TGG + Chr3:100752533-100752552 None:intergenic 5.0%
ACTTCCACGTGAAGGTTGCT+GGG - Chr3:100752797-100752816 MsG0380017797.01.T01:CDS 50.0%
ATAAAATGGAAGCGGGGCCA+AGG + Chr3:100753300-100753319 None:intergenic 50.0%
ATAACCCAGCAACCTTCACG+TGG + Chr3:100752804-100752823 None:intergenic 50.0%
TAAAATGGAAGCGGGGCCAA+GGG + Chr3:100753299-100753318 None:intergenic 50.0%
TACTTCCACGTGAAGGTTGC+TGG - Chr3:100752796-100752815 MsG0380017797.01.T01:CDS 50.0%
! TTGGCCCCGCTTCCATTTTA+TGG - Chr3:100753299-100753318 MsG0380017797.01.T01:five_prime_UTR 50.0%
AAAATGGAAGCGGGGCCAAG+GGG + Chr3:100753298-100753317 None:intergenic 55.0%
! GAGAGTGCACAGATGCCCCT+TGG - Chr3:100753280-100753299 MsG0380017797.01.T01:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 100752383 100753324 100752383 ID=MsG0380017797.01;Name=MsG0380017797.01
Chr3 mRNA 100752383 100753324 100752383 ID=MsG0380017797.01.T01;Parent=MsG0380017797.01;Name=MsG0380017797.01.T01;_AED=0.45;_eAED=0.45;_QI=273|1|0.75|1|0.33|0.5|4|77|57
Chr3 exon 100752383 100752499 100752383 ID=MsG0380017797.01.T01:exon:27428;Parent=MsG0380017797.01.T01
Chr3 exon 100752726 100752929 100752726 ID=MsG0380017797.01.T01:exon:27427;Parent=MsG0380017797.01.T01
Chr3 exon 100753015 100753107 100753015 ID=MsG0380017797.01.T01:exon:27426;Parent=MsG0380017797.01.T01
Chr3 exon 100753215 100753324 100753215 ID=MsG0380017797.01.T01:exon:27425;Parent=MsG0380017797.01.T01
Chr3 five_prime_UTR 100753215 100753324 100753215 ID=MsG0380017797.01.T01:five_prime_utr;Parent=MsG0380017797.01.T01
Chr3 five_prime_UTR 100753015 100753107 100753015 ID=MsG0380017797.01.T01:five_prime_utr;Parent=MsG0380017797.01.T01
Chr3 five_prime_UTR 100752860 100752929 100752860 ID=MsG0380017797.01.T01:five_prime_utr;Parent=MsG0380017797.01.T01
Chr3 CDS 100752726 100752859 100752726 ID=MsG0380017797.01.T01:cds;Parent=MsG0380017797.01.T01
Chr3 CDS 100752460 100752499 100752460 ID=MsG0380017797.01.T01:cds;Parent=MsG0380017797.01.T01
Chr3 three_prime_UTR 100752383 100752459 100752383 ID=MsG0380017797.01.T01:three_prime_utr;Parent=MsG0380017797.01.T01
Gene Sequence

>MsG0380017797.01.T01

ATGTTTCTCCTTCTCTTGTTTTGGAACTTTTACATAGAACTGCACGTGTCATCAAAGATTACCTTGGTGTTCTTAATGAAGACTCTTTTCGAAAGAATTTTCTCCTTGTCTATGAGCTTCTTGATGAAGTCATTGATTTTGGTTATGTGCAAACAACATCTACTGAGGTTTTGA

Protein sequence

>MsG0380017797.01.T01

MFLLLLFWNFYIELHVSSKITLVFLMKTLFERIFSLSMSFLMKSLILVMCKQHLLRF*