AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043162.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043162.01.T01 MTR_8g030830 93.085 188 13 0 1 188 1 188 2.78e-120 343
MsG0880043162.01.T01 MTR_8g446960 94.149 188 11 0 1 188 1 188 2.75e-113 325
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043162.01.T01 AT1G13870 72.340 188 50 2 1 188 1 186 6.92e-96 281

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATTCTTTGAATAACATTA+AGG 0.212951 8:-19995205 MsG0880043162.01.T01:CDS
CGATGCAATATTTGAAGATT+TGG 0.217452 8:-19995049 MsG0880043162.01.T01:CDS
AGAATTTAAGAGGAGTTCTT+AGG 0.298956 8:-19995272 MsG0880043162.01.T01:CDS
TGGTGTTTGGCTCGTGCTGC+AGG 0.319369 8:-19995165 MsG0880043162.01.T01:CDS
TCCCATCTATTTCTTCTCTC+TGG 0.324920 8:+19995009 None:intergenic
CCGATATGAGCTATGGTGTT+TGG 0.374786 8:-19995178 MsG0880043162.01.T01:CDS
TGACGATTCTTTGAGAGCTT+CGG 0.376709 8:+19995380 None:intergenic
GGCTAGACTTAGCGCAGCTT+TGG 0.378208 8:+19995401 None:intergenic
AGGATGATGGTTGTAGAAAA+TGG 0.390890 8:-19995110 MsG0880043162.01.T01:CDS
GATTCTTTGAATAACATTAA+GGG 0.396934 8:-19995204 MsG0880043162.01.T01:CDS
AACCAGAGAGAAGAAATAGA+TGG 0.404380 8:-19995011 MsG0880043162.01.T01:CDS
TACTGTGATGTTGAGGATGA+TGG 0.439873 8:-19995123 MsG0880043162.01.T01:CDS
CCATCAGAGAAGAATTTAAG+AGG 0.458052 8:-19995282 MsG0880043162.01.T01:CDS
TTCGTAGTTTCAAATGGCAC+TGG 0.481368 8:-19995457 None:intergenic
TTCTTAGGTCAGAAGTTGAC+AGG 0.485003 8:-19995257 MsG0880043162.01.T01:CDS
CCTCTTAAATTCTTCTCTGA+TGG 0.488428 8:+19995282 None:intergenic
AAGCTTGTTTCCATCTTGAT+CGG 0.496584 8:-19995326 MsG0880043162.01.T01:CDS
CAATATTTGAAGATTTGGTA+AGG 0.503933 8:-19995044 MsG0880043162.01.T01:CDS
AACTTTGATTCCGATCAAGA+TGG 0.519631 8:+19995316 None:intergenic
TTTGTTTGAGTTGAAATCAT+CGG 0.521912 8:-19994980 MsG0880043162.01.T01:CDS
CCAAACACCATAGCTCATAT+CGG 0.531120 8:+19995178 None:intergenic
AATCAAGAGCGAAGGGAGAA+AGG 0.534973 8:-19995087 MsG0880043162.01.T01:CDS
ACCAGAGAGAAGAAATAGAT+GGG 0.553983 8:-19995010 MsG0880043162.01.T01:CDS
TGTTGTTTACTGTGATGTTG+AGG 0.554593 8:-19995130 MsG0880043162.01.T01:CDS
TTGCAGTATCTTCACATCGC+GGG 0.554850 8:+19994880 None:intergenic
GGTGTTTGGCTCGTGCTGCA+GGG 0.562065 8:-19995164 MsG0880043162.01.T01:CDS
GATGATTTCAACTCAAACAA+AGG 0.581112 8:+19994982 None:intergenic
TATTTGAAGATTTGGTAAGG+AGG 0.589953 8:-19995041 MsG0880043162.01.T01:CDS
AGGGTTACCGATATGAGCTA+TGG 0.591762 8:-19995185 MsG0880043162.01.T01:CDS
GAAAATGGAATCAAGAGCGA+AGG 0.596859 8:-19995095 MsG0880043162.01.T01:CDS
AAAATGGAATCAAGAGCGAA+GGG 0.624157 8:-19995094 MsG0880043162.01.T01:CDS
GACAGCATCATCTACAACGG+AGG 0.629029 8:+19994933 None:intergenic
ATGGCACTGGTTGTAATATG+TGG 0.645253 8:-19995444 MsG0880043162.01.T01:CDS
ATATGTGGTCAACCATGCAG+TGG 0.681649 8:-19995429 MsG0880043162.01.T01:CDS
GTTGCAGTATCTTCACATCG+CGG 0.688808 8:+19994879 None:intergenic
AGAGACAGCATCATCTACAA+CGG 0.703655 8:+19994930 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTCTTTGAATAACATTAA+GGG - Chr8:19995131-19995150 MsG0880043162.01.T01:CDS 20.0%
!! TGATTCTTTGAATAACATTA+AGG - Chr8:19995130-19995149 MsG0880043162.01.T01:CDS 20.0%
!! CAATATTTGAAGATTTGGTA+AGG - Chr8:19995291-19995310 MsG0880043162.01.T01:CDS 25.0%
!!! TTTGTTTGAGTTGAAATCAT+CGG - Chr8:19995355-19995374 MsG0880043162.01.T01:CDS 25.0%
AGAATTTAAGAGGAGTTCTT+AGG - Chr8:19995063-19995082 MsG0880043162.01.T01:CDS 30.0%
CGATGCAATATTTGAAGATT+TGG - Chr8:19995286-19995305 MsG0880043162.01.T01:CDS 30.0%
GATGATTTCAACTCAAACAA+AGG + Chr8:19995356-19995375 None:intergenic 30.0%
TCTCTTACATAACCAAAAAG+TGG - Chr8:19995423-19995442 MsG0880043162.01.T01:CDS 30.0%
! TATTTGAAGATTTGGTAAGG+AGG - Chr8:19995294-19995313 MsG0880043162.01.T01:CDS 30.0%
AAAATGGAATCAAGAGCGAA+GGG - Chr8:19995241-19995260 MsG0880043162.01.T01:CDS 35.0%
AACCAGAGAGAAGAAATAGA+TGG - Chr8:19995324-19995343 MsG0880043162.01.T01:CDS 35.0%
AACTTTGATTCCGATCAAGA+TGG + Chr8:19995022-19995041 None:intergenic 35.0%
ACCAGAGAGAAGAAATAGAT+GGG - Chr8:19995325-19995344 MsG0880043162.01.T01:CDS 35.0%
CCATCAGAGAAGAATTTAAG+AGG - Chr8:19995053-19995072 MsG0880043162.01.T01:CDS 35.0%
CCTCTTAAATTCTTCTCTGA+TGG + Chr8:19995056-19995075 None:intergenic 35.0%
! AAGCTTGTTTCCATCTTGAT+CGG - Chr8:19995009-19995028 MsG0880043162.01.T01:CDS 35.0%
! AGGATGATGGTTGTAGAAAA+TGG - Chr8:19995225-19995244 MsG0880043162.01.T01:CDS 35.0%
! TGTTGTTTACTGTGATGTTG+AGG - Chr8:19995205-19995224 MsG0880043162.01.T01:CDS 35.0%
AGAGACAGCATCATCTACAA+CGG + Chr8:19995408-19995427 None:intergenic 40.0%
CCAAACACCATAGCTCATAT+CGG + Chr8:19995160-19995179 None:intergenic 40.0%
GAAAATGGAATCAAGAGCGA+AGG - Chr8:19995240-19995259 MsG0880043162.01.T01:CDS 40.0%
TACTGTGATGTTGAGGATGA+TGG - Chr8:19995212-19995231 MsG0880043162.01.T01:CDS 40.0%
TTCTTAGGTCAGAAGTTGAC+AGG - Chr8:19995078-19995097 MsG0880043162.01.T01:CDS 40.0%
! TCCCATCTATTTCTTCTCTC+TGG + Chr8:19995329-19995348 None:intergenic 40.0%
! TGACGATTCTTTGAGAGCTT+CGG + Chr8:19994958-19994977 None:intergenic 40.0%
!! ATGGCACTGGTTGTAATATG+TGG - Chr8:19994891-19994910 MsG0880043162.01.T01:CDS 40.0%
!!! GGGTTTTTGAGTCCACTTTT+TGG + Chr8:19995438-19995457 None:intergenic 40.0%
AATCAAGAGCGAAGGGAGAA+AGG - Chr8:19995248-19995267 MsG0880043162.01.T01:CDS 45.0%
AGGGTTACCGATATGAGCTA+TGG - Chr8:19995150-19995169 MsG0880043162.01.T01:CDS 45.0%
ATATGTGGTCAACCATGCAG+TGG - Chr8:19994906-19994925 MsG0880043162.01.T01:CDS 45.0%
CCGATATGAGCTATGGTGTT+TGG - Chr8:19995157-19995176 MsG0880043162.01.T01:CDS 45.0%
!! GCTTTGGATTTTCCACTGCA+TGG + Chr8:19994921-19994940 None:intergenic 45.0%
GACAGCATCATCTACAACGG+AGG + Chr8:19995405-19995424 None:intergenic 50.0%
GGCTAGACTTAGCGCAGCTT+TGG + Chr8:19994937-19994956 None:intergenic 55.0%
! GGTGTTTGGCTCGTGCTGCA+GGG - Chr8:19995171-19995190 MsG0880043162.01.T01:CDS 60.0%
! TGGTGTTTGGCTCGTGCTGC+AGG - Chr8:19995170-19995189 MsG0880043162.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 19994891 19995466 19994891 ID=MsG0880043162.01;Name=MsG0880043162.01
Chr8 mRNA 19994891 19995466 19994891 ID=MsG0880043162.01.T01;Parent=MsG0880043162.01;Name=MsG0880043162.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|191
Chr8 exon 19994891 19995466 19994891 ID=MsG0880043162.01.T01:exon:5310;Parent=MsG0880043162.01.T01
Chr8 CDS 19994891 19995466 19994891 ID=MsG0880043162.01.T01:cds;Parent=MsG0880043162.01.T01
Gene Sequence

>MsG0880043162.01.T01

ATGGCACTGGTTGTAATATGTGGTCAACCATGCAGTGGAAAATCCAAAGCTGCGCTAAGTCTAGCCGAAGCTCTCAAAGAATCGTCAGAATTCAAACATCAAGTGAGAATCATAGATGAAGCTTGTTTCCATCTTGATCGGAATCAAAGTTATGCAAATATGCCATCAGAGAAGAATTTAAGAGGAGTTCTTAGGTCAGAAGTTGACAGGTCTCTGTCAAAAGATACTGTTATTATCGTTGATTCTTTGAATAACATTAAGGGTTACCGATATGAGCTATGGTGTTTGGCTCGTGCTGCAGGGATTAGATACTGTGTTGTTTACTGTGATGTTGAGGATGATGGTTGTAGAAAATGGAATCAAGAGCGAAGGGAGAAAGGTGAAGATAATTATGACGATGCAATATTTGAAGATTTGGTAAGGAGGTTTGAGAAACCAGAGAGAAGAAATAGATGGGATTCACCTTTGTTTGAGTTGAAATCATCGGCATCGTCTTCTTCGTCTGATTCTGCCTCCGTTGTAGATGATGCTGTCTCTTACATAACCAAAAAGTGGACTCAAAAACCCGCGATGTGA

Protein sequence

>MsG0880043162.01.T01

MALVVICGQPCSGKSKAALSLAEALKESSEFKHQVRIIDEACFHLDRNQSYANMPSEKNLRGVLRSEVDRSLSKDTVIIVDSLNNIKGYRYELWCLARAAGIRYCVVYCDVEDDGCRKWNQERREKGEDNYDDAIFEDLVRRFEKPERRNRWDSPLFELKSSASSSSSDSASVVDDAVSYITKKWTQKPAM*