AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0338340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338340 MtrunA17_Chr2g0326001 86.545 275 15 5 1 253 1 275 5.21e-148 415
Msa0338340 MtrunA17_Chr4g0030051 47.970 271 79 13 17 248 18 265 8.66e-51 167
Msa0338340 MtrunA17_Chr3g0127201 69.118 68 15 2 29 90 16 83 1.78e-24 99.0
Msa0338340 MtrunA17_Chr2g0328021 37.580 157 75 6 8 158 31 170 5.17e-22 92.4
Msa0338340 MtrunA17_Chr1g0188851 50.549 91 35 4 8 88 7 97 1.38e-21 91.3
Msa0338340 MtrunA17_Chr8g0346551 50.505 99 29 5 23 110 9 98 6.73e-21 89.0
Msa0338340 MtrunA17_Chr4g0010151 44.186 129 44 4 7 120 62 177 2.13e-20 89.0
Msa0338340 MtrunA17_Chr3g0126201 61.972 71 21 2 24 88 35 105 2.48e-20 88.6
Msa0338340 MtrunA17_Chr3g0117601 45.926 135 54 7 11 134 29 155 3.07e-20 88.2
Msa0338340 MtrunA17_Chr4g0031411 53.261 92 30 4 22 100 57 148 6.88e-20 87.4
Msa0338340 MtrunA17_Chr4g0047001 58.108 74 25 2 42 109 57 130 3.08e-19 85.9
Msa0338340 MtrunA17_Chr8g0374251 51.852 81 33 2 37 111 65 145 3.25e-18 82.8
Msa0338340 MtrunA17_Chr8g0367161 47.674 86 39 2 29 108 21 106 3.30e-18 82.8
Msa0338340 MtrunA17_Chr8g0374111 52.874 87 32 4 11 90 1 85 1.62e-17 79.0
Msa0338340 MtrunA17_Chr1g0177251 66.667 54 12 1 41 88 13 66 4.30e-17 79.0
Msa0338340 MtrunA17_Chr7g0237921 53.425 73 28 2 22 88 58 130 4.65e-17 79.3
Msa0338340 MtrunA17_Chr5g0411951 63.636 55 14 2 36 84 39 93 1.63e-16 77.8
Msa0338340 MtrunA17_Chr2g0282441 62.069 58 13 3 34 85 22 76 1.98e-16 77.8
Msa0338340 MtrunA17_Chr3g0092111 56.164 73 25 3 26 92 123 194 2.60e-16 78.2
Msa0338340 MtrunA17_Chr2g0282051 66.667 57 12 2 38 88 44 99 2.71e-16 76.6
Msa0338340 MtrunA17_Chr7g0223311 60.000 65 20 2 39 97 76 140 3.25e-16 77.4
Msa0338340 MtrunA17_Chr4g0047761 70.175 57 10 3 38 88 70 125 6.25e-16 75.9
Msa0338340 MtrunA17_Chr7g0217581 50.000 84 28 3 21 90 205 288 1.64e-15 75.9
Msa0338340 MtrunA17_Chr8g0341671 56.944 72 24 3 28 92 8 79 2.97e-15 72.8
Msa0338340 MtrunA17_Chr5g0417571 55.072 69 24 3 28 90 127 194 5.21e-15 73.6
Msa0338340 MtrunA17_Chr4g0042481 53.846 65 24 2 29 87 111 175 1.66e-14 72.8
Msa0338340 MtrunA17_Chr4g0061761 62.264 53 14 2 38 84 38 90 2.35e-14 71.6
Msa0338340 MtrunA17_Chr8g0355261 51.429 70 27 3 26 89 105 173 2.92e-14 72.0
Msa0338340 MtrunA17_Chr6g0453321 54.839 62 22 2 35 90 131 192 6.36e-14 70.9
Msa0338340 MtrunA17_Chr6g0460721 43.750 80 38 3 15 88 65 143 7.29e-14 70.1
Msa0338340 MtrunA17_Chr7g0254161 54.237 59 21 2 35 87 96 154 8.34e-14 70.5
Msa0338340 MtrunA17_Chr2g0282511 66.000 50 11 2 39 82 44 93 9.08e-14 69.7
Msa0338340 MtrunA17_Chr6g0460731 37.097 124 68 4 15 132 72 191 1.04e-13 70.1
Msa0338340 MtrunA17_Chr2g0308011 68.000 50 10 2 39 82 36 85 1.05e-13 68.2
Msa0338340 MtrunA17_Chr5g0417551 40.870 115 53 5 8 109 96 208 7.09e-13 67.8
Msa0338340 MtrunA17_Chr5g0417561 51.351 74 30 2 36 103 115 188 4.37e-12 65.1
Msa0338340 MtrunA17_Chr2g0283811 54.386 57 20 2 37 87 44 100 4.72e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338340 AT5G60200.1 35.217 230 92 8 26 240 33 220 1.11e-29 112
Msa0338340 AT2G28510.1 41.333 225 95 11 38 240 46 255 3.26e-27 106
Msa0338340 AT3G45610.1 32.540 252 105 10 11 239 1 210 1.33e-26 103
Msa0338340 AT4G24060.1 65.152 66 17 2 29 88 42 107 6.93e-22 93.2
Msa0338340 AT3G55370.1 52.632 95 33 4 12 99 52 141 3.73e-21 90.9
Msa0338340 AT3G55370.3 52.632 95 33 4 12 99 97 186 4.27e-21 91.7
Msa0338340 AT3G55370.2 52.632 95 33 4 12 99 52 141 5.11e-21 91.3
Msa0338340 AT3G61850.1 35.023 217 109 9 33 221 67 279 2.10e-20 88.6
Msa0338340 AT3G61850.2 35.023 217 109 9 33 221 55 267 2.19e-20 88.2
Msa0338340 AT3G61850.3 35.023 217 109 9 33 221 55 267 2.19e-20 88.2
Msa0338340 AT3G61850.5 62.500 64 18 1 33 90 55 118 1.23e-19 86.7
Msa0338340 AT3G61850.4 62.500 64 18 1 33 90 67 130 1.53e-19 86.7
Msa0338340 AT2G37590.1 54.054 74 28 2 22 89 71 144 3.19e-19 85.9
Msa0338340 AT5G60850.1 62.500 72 17 3 37 98 48 119 3.31e-19 85.5
Msa0338340 AT5G02460.1 63.934 61 16 2 34 88 89 149 1.00e-18 85.1
Msa0338340 AT1G64620.1 60.656 61 18 1 29 83 38 98 1.08e-17 81.6
Msa0338340 AT4G00940.1 61.667 60 17 1 35 88 63 122 1.24e-17 80.9
Msa0338340 AT4G00940.3 61.667 60 17 1 35 88 63 122 1.24e-17 80.9
Msa0338340 AT4G00940.2 61.667 60 17 1 35 88 63 122 1.24e-17 80.9
Msa0338340 AT5G65590.1 53.659 82 28 3 24 95 21 102 1.25e-17 81.3
Msa0338340 AT1G51700.1 48.421 95 39 4 20 107 16 107 3.10e-17 77.8
Msa0338340 AT3G52440.1 60.000 65 18 2 37 95 22 84 1.06e-16 77.4
Msa0338340 AT1G07640.2 43.678 87 42 2 9 88 45 131 1.13e-16 78.6
Msa0338340 AT3G52440.2 60.000 65 18 2 37 95 41 103 1.17e-16 77.8
Msa0338340 AT1G07640.3 43.678 87 42 2 9 88 53 139 1.35e-16 78.6
Msa0338340 AT2G28810.2 62.500 56 15 2 36 85 73 128 2.20e-16 77.8
Msa0338340 AT2G28810.1 62.500 56 15 2 36 85 90 145 3.02e-16 77.4
Msa0338340 AT5G62940.1 57.377 61 20 2 30 84 63 123 3.72e-16 77.4
Msa0338340 AT1G21340.1 63.158 57 15 2 39 89 37 93 3.96e-16 76.3
Msa0338340 AT2G46590.1 48.000 125 53 4 29 143 59 181 4.21e-16 77.4
Msa0338340 AT2G46590.2 48.000 125 53 4 29 143 71 193 4.66e-16 77.4
Msa0338340 AT1G07640.1 50.667 75 30 2 21 88 1 75 6.95e-16 75.9
Msa0338340 AT5G62430.1 47.253 91 41 3 26 109 39 129 1.07e-15 75.5
Msa0338340 AT1G28310.1 65.455 55 13 2 40 88 27 81 1.52e-15 75.1
Msa0338340 AT1G28310.2 65.455 55 13 2 40 88 51 105 1.87e-15 75.1
Msa0338340 AT5G39660.3 48.810 84 31 4 22 99 124 201 9.08e-15 73.9
Msa0338340 AT5G39660.1 48.810 84 31 4 22 99 124 201 9.08e-15 73.9
Msa0338340 AT5G39660.2 48.810 84 31 4 22 99 124 201 9.08e-15 73.9
Msa0338340 AT5G66940.1 54.930 71 25 3 32 96 25 94 9.53e-15 71.6
Msa0338340 AT4G21050.1 50.725 69 26 2 33 95 19 85 9.92e-15 71.2
Msa0338340 AT3G47500.1 58.065 62 20 2 35 90 105 166 1.16e-14 73.6
Msa0338340 AT3G21270.1 66.038 53 12 2 40 86 29 81 2.07e-14 70.5
Msa0338340 AT3G50410.1 56.667 60 20 2 37 90 27 86 2.17e-14 71.2
Msa0338340 AT4G38000.1 52.308 65 24 3 30 87 31 95 5.24e-14 70.1
Msa0338340 AT1G69570.1 55.932 59 20 2 35 87 127 185 1.08e-13 70.5
Msa0338340 AT1G26790.1 45.946 74 34 2 20 87 91 164 1.41e-13 70.1
Msa0338340 AT1G47655.1 57.895 57 18 2 32 82 21 77 2.96e-13 67.4
Msa0338340 AT4G21080.1 37.143 105 59 2 17 114 1 105 5.87e-13 67.0
Msa0338340 AT2G34140.1 52.542 59 22 2 35 87 53 111 8.54e-13 65.1
Msa0338340 AT1G29160.1 52.542 59 22 2 35 87 57 115 2.49e-12 63.9
Msa0338340 AT4G21040.1 38.679 106 56 4 17 114 1 105 2.35e-11 62.4

Find 74 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATAAACCATGGATTTA+TGG 0.149923 2_4:+58601953 Msa0338340:CDS
GCTTGTTCTGGTTGAGGTTT+TGG 0.170758 2_4:-58601549 None:intergenic
ATCCATAAATCCATGGTTTA+TGG 0.190178 2_4:-58601955 None:intergenic
TTCCATTACCAACCTCATTT+TGG 0.230948 2_4:+58600481 Msa0338340:five_prime_UTR
GATTCATTCATCTTATTGTT+TGG 0.284874 2_4:+58600625 Msa0338340:five_prime_UTR
ATTCCTTAGGGTTCCTCCTT+TGG 0.285324 2_4:-58601679 None:intergenic
ATTAGAAGTGGATTATTTCT+TGG 0.296220 2_4:+58601981 Msa0338340:CDS
GGACAGTTCAAAGCTTGTTC+TGG 0.303186 2_4:-58601561 None:intergenic
GTTGTTATAGTAACAGAATT+TGG 0.321643 2_4:-58601604 None:intergenic
GAATGAGGGAGATTGTTAAA+TGG 0.326085 2_4:-58601801 None:intergenic
AGTGGATTATTTCTTGGAAA+TGG 0.336042 2_4:+58601987 Msa0338340:CDS
GGGTTTCCATGGCAGCTAAA+TGG 0.341098 2_4:+58602215 Msa0338340:CDS
TATAGGTAGGTATATATAAT+AGG 0.347193 2_4:-58600446 None:intergenic
ATGTATGGTGTGTATGGAAA+TGG 0.354795 2_4:+58602038 Msa0338340:CDS
TCACTCTCTCTCTTGGGATT+GGG 0.359287 2_4:+58600419 None:intergenic
CAAGATTTGCAGAAATATCT+TGG 0.375372 2_4:-58601642 None:intergenic
ATTGTTAATTTCTTGATCAT+AGG 0.377525 2_4:-58602135 None:intergenic
AAAGCAATCATGTGGACAAA+TGG 0.379363 2_4:+58601875 Msa0338340:CDS
ATCACTCTCTCTCTTGGGAT+TGG 0.387605 2_4:+58600418 None:intergenic
CATTCATCTTATTGTTTGGA+TGG 0.393564 2_4:+58600629 Msa0338340:exon
CTAACAAAGGTCTTATCTAA+TGG 0.393902 2_4:-58602959 None:intergenic
TCATCAATGGAAAACATGTT+AGG 0.412061 2_4:+58601498 Msa0338340:CDS
AATGAGGGAGATTGTTAAAT+GGG 0.423268 2_4:-58601800 None:intergenic
TTGTTAATTTCTTGATCATA+GGG 0.429595 2_4:-58602134 None:intergenic
CAGAATATGTATGGTGTGTA+TGG 0.444454 2_4:+58602032 Msa0338340:intron
TTCAAAGCTTGTTCTGGTTG+AGG 0.444926 2_4:-58601555 None:intergenic
CACCAACAGGAACATTCCTT+AGG 0.445109 2_4:-58601692 None:intergenic
TGGAAGCAAAGGTGCTGTAT+AGG 0.448996 2_4:-58600463 None:intergenic
TTGTTAAATGGGCTGAGATT+TGG 0.449385 2_4:-58601789 None:intergenic
TGTATGGTGTGTATGGAAAT+GGG 0.450678 2_4:+58602039 Msa0338340:CDS
AAGCAATCATGTGGACAAAT+GGG 0.452916 2_4:+58601876 Msa0338340:CDS
TAATGCTAATGCAAAATCAT+TGG 0.457564 2_4:-58601841 None:intergenic
TCCTTCTTGTGGACAACCTC+AGG 0.458211 2_4:+58600653 Msa0338340:CDS
GTGTTGGTTGAGTTGCATCT+TGG 0.458480 2_4:-58601582 None:intergenic
AATAATGTTCAGAATATGTA+TGG 0.460682 2_4:+58602023 Msa0338340:intron
ATCCAAAATGAGGTTGGTAA+TGG 0.464507 2_4:-58600483 None:intergenic
TTTCTGCATCCACCACCAAC+AGG 0.469608 2_4:-58601705 None:intergenic
GTTCCTCCATTTAGCTGCCA+TGG 0.475903 2_4:-58602221 None:intergenic
ATGCAAATAACAATGCTTGT+GGG 0.493320 2_4:+58602090 Msa0338340:CDS
AATGTCGAGCCAATCATCAA+TGG 0.508256 2_4:+58601485 Msa0338340:CDS
CAAAGGTCTTATCTAATGGC+TGG 0.511950 2_4:-58602955 None:intergenic
GACAATGGTGAAGTAAATAG+TGG 0.512707 2_4:+58602062 Msa0338340:CDS
AGGAATGTTCCTGTTGGTGG+TGG 0.512948 2_4:+58601696 Msa0338340:CDS
TTTATGGATGCAATTAGAAG+TGG 0.515360 2_4:+58601969 Msa0338340:CDS
CTAAGGAATGTTCCTGTTGG+TGG 0.527080 2_4:+58601693 Msa0338340:CDS
ATAGTAACAGAATTTGGTGT+TGG 0.529356 2_4:-58601598 None:intergenic
TAATTGCATCCATAAATCCA+TGG 0.534717 2_4:-58601962 None:intergenic
GTTTGGATGGATCCTTCTTG+TGG 0.537133 2_4:+58600642 Msa0338340:exon
ACCAACAGGAACATTCCTTA+GGG 0.541748 2_4:-58601691 None:intergenic
AATGCAAATAACAATGCTTG+TGG 0.543604 2_4:+58602089 Msa0338340:CDS
GTGCTTGGAGTGAAACTGAA+TGG 0.547945 2_4:-58600581 None:intergenic
TATGATCATAAGATGAATGA+GGG 0.553710 2_4:-58601815 None:intergenic
AGCAAGAAATGTGCAGTGGA+AGG 0.565157 2_4:+58602162 Msa0338340:CDS
TTATGATCATAAGATGAATG+AGG 0.565287 2_4:-58601816 None:intergenic
ACCTGAGGTTGTCCACAAGA+AGG 0.573402 2_4:-58600654 None:intergenic
ACCCTAAGGAATGTTCCTGT+TGG 0.579058 2_4:+58601690 Msa0338340:CDS
CATCATCATCATCTTCAAAG+AGG 0.581905 2_4:+58601745 Msa0338340:CDS
TGCAAATAACAATGCTTGTG+GGG 0.582187 2_4:+58602091 Msa0338340:CDS
TGTAGAAGGTATTGGACCAA+AGG 0.585230 2_4:+58601663 Msa0338340:intron
TGCTTGGAGTGAAACTGAAT+GGG 0.586484 2_4:-58600580 None:intergenic
CTCTTTGAAGATGATGATGA+TGG 0.589822 2_4:-58601744 None:intergenic
GGACCAAAGGAGGAACCCTA+AGG 0.594931 2_4:+58601676 Msa0338340:CDS
GAGGTTGGTAATGGAAGCAA+AGG 0.595868 2_4:-58600474 None:intergenic
GCAAGAAATGTGCAGTGGAA+GGG 0.598256 2_4:+58602163 Msa0338340:CDS
GTGTATGGAAATGGGGACAA+TGG 0.598902 2_4:+58602047 Msa0338340:CDS
AATCATTCAACCATAAACCA+TGG 0.606118 2_4:+58601945 Msa0338340:CDS
GCTAAATGGAGGAACAAACA+TGG 0.611119 2_4:+58602229 Msa0338340:CDS
AGAAGGTATTGGACCAAAGG+AGG 0.622045 2_4:+58601666 Msa0338340:intron
GTATGGTGTGTATGGAAATG+GGG 0.629075 2_4:+58602040 Msa0338340:CDS
AGCAAAGGTGCTGTATAGGT+AGG 0.632763 2_4:-58600459 None:intergenic
AGGCTTCAAAAGCAATCATG+TGG 0.635965 2_4:+58601867 Msa0338340:CDS
TTTCCATGGCAGCTAAATGG+AGG 0.645564 2_4:+58602218 Msa0338340:CDS
CTAAATGGAGGAACAAACAT+GGG 0.651200 2_4:+58602230 Msa0338340:CDS
GTGAAGCAAGAAATGTGCAG+TGG 0.683660 2_4:+58602158 Msa0338340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATTAAAGTACTAAAAT+TGG + chr2_4:58601371-58601390 Msa0338340:intron 10.0%
!!! AGAGACAAATTTTATTTTTT+TGG - chr2_4:58601333-58601352 None:intergenic 15.0%
!!! TTAATTTTAACATTTAGCAA+CGG - chr2_4:58601360-58601379 None:intergenic 15.0%
!! AATTATACACAAAATACAAT+TGG - chr2_4:58601400-58601419 None:intergenic 15.0%
!!! TTGTATTTTGTGTATAATTT+TGG + chr2_4:58601401-58601420 Msa0338340:intron 15.0%
!! TATAGGTAGGTATATATAAT+AGG - chr2_4:58600449-58600468 None:intergenic 20.0%
!!! AATATTATGTTTGAAGTTTG+AGG + chr2_4:58600749-58600768 Msa0338340:intron 20.0%
!!! ATATTATGTTTGAAGTTTGA+GGG + chr2_4:58600750-58600769 Msa0338340:intron 20.0%
!!! CAATATATTTTGTGTAAAGA+TGG - chr2_4:58600845-58600864 None:intergenic 20.0%
!! AATTAGATATATAGAAGACA+TGG - chr2_4:58600964-58600983 None:intergenic 20.0%
!!! TTGCAATTTATGTATTTTGT+TGG - chr2_4:58601070-58601089 None:intergenic 20.0%
!! AGTTGAATCAAAGTTATAAT+AGG - chr2_4:58601289-58601308 None:intergenic 20.0%
!! AATAATGTTCAGAATATGTA+TGG + chr2_4:58602023-58602042 Msa0338340:intron 20.0%
!! TTGTTAATTTCTTGATCATA+GGG - chr2_4:58602137-58602156 None:intergenic 20.0%
!! ATTGTTAATTTCTTGATCAT+AGG - chr2_4:58602138-58602157 None:intergenic 20.0%
!!! ATTAGTGAAAGTAAAGTTTT+TGG + chr2_4:58602191-58602210 Msa0338340:CDS 20.0%
!! TAGAATCAAAAGCTATATTA+CGG + chr2_4:58602332-58602351 Msa0338340:intron 20.0%
!!! TCATATTTTAGTCAAACATT+AGG - chr2_4:58602497-58602516 None:intergenic 20.0%
!!! TTAATAGAGATTGATTTATC+TGG + chr2_4:58602560-58602579 Msa0338340:intron 20.0%
!! GAAAATAATACAAACAAACA+AGG - chr2_4:58602609-58602628 None:intergenic 20.0%
! GATTCATTCATCTTATTGTT+TGG + chr2_4:58600625-58600644 Msa0338340:five_prime_UTR 25.0%
! TATACTTAAACTGCTCTTAT+GGG - chr2_4:58600805-58600824 None:intergenic 25.0%
! TTATACTTAAACTGCTCTTA+TGG - chr2_4:58600806-58600825 None:intergenic 25.0%
!! AATTTATATGTCTTCTAGCT+TGG + chr2_4:58600980-58600999 Msa0338340:intron 25.0%
! ACAGTATAAAAGATGAAGAT+GGG - chr2_4:58601037-58601056 None:intergenic 25.0%
! AACAGTATAAAAGATGAAGA+TGG - chr2_4:58601038-58601057 None:intergenic 25.0%
! GAGTAATGTGTATTTGTATA+TGG + chr2_4:58601248-58601267 Msa0338340:intron 25.0%
!! TTTTATTAGATTTGCACTCA+TGG + chr2_4:58601441-58601460 Msa0338340:intron 25.0%
! GTTGTTATAGTAACAGAATT+TGG - chr2_4:58601607-58601626 None:intergenic 25.0%
! TATGATCATAAGATGAATGA+GGG - chr2_4:58601818-58601837 None:intergenic 25.0%
! TTATGATCATAAGATGAATG+AGG - chr2_4:58601819-58601838 None:intergenic 25.0%
! TAATGCTAATGCAAAATCAT+TGG - chr2_4:58601844-58601863 None:intergenic 25.0%
!!! ATTAGAAGTGGATTATTTCT+TGG + chr2_4:58601981-58602000 Msa0338340:CDS 25.0%
!!! AGCTTTTGATTCTACATTAA+GGG - chr2_4:58602326-58602345 None:intergenic 25.0%
!!! TAGCTTTTGATTCTACATTA+AGG - chr2_4:58602327-58602346 None:intergenic 25.0%
!! TCAATTCTCTTAAGTAGTTT+TGG + chr2_4:58602536-58602555 Msa0338340:intron 25.0%
! AAAAAATGTGACATCTTAGA+GGG - chr2_4:58602647-58602666 None:intergenic 25.0%
! TTTATCTTGGAAAAATCAAC+AGG - chr2_4:58602925-58602944 None:intergenic 25.0%
TGAAAAATCCAAAATGAGGT+TGG - chr2_4:58600492-58600511 None:intergenic 30.0%
AGAGTGAAAAATCCAAAATG+AGG - chr2_4:58600496-58600515 None:intergenic 30.0%
TGGGAAAAAAGAAGAAGAAA+GGG - chr2_4:58600564-58600583 None:intergenic 30.0%
ATGGGAAAAAAGAAGAAGAA+AGG - chr2_4:58600565-58600584 None:intergenic 30.0%
CATTCATCTTATTGTTTGGA+TGG + chr2_4:58600629-58600648 Msa0338340:exon 30.0%
TAGCTTGGAAATTGAAGTTA+GGG + chr2_4:58600995-58601014 Msa0338340:intron 30.0%
! GAAGGTATATACTAGTACTT+AGG - chr2_4:58601104-58601123 None:intergenic 30.0%
!!! AGTAGCACTTTTTTTGAAGA+AGG - chr2_4:58601122-58601141 None:intergenic 30.0%
TACTCACACAACAAAAGTTA+GGG - chr2_4:58601233-58601252 None:intergenic 30.0%
TTACTCACACAACAAAAGTT+AGG - chr2_4:58601234-58601253 None:intergenic 30.0%
!! ACATGTTTTCCATTGATGAT+TGG - chr2_4:58601497-58601516 None:intergenic 30.0%
TCATCAATGGAAAACATGTT+AGG + chr2_4:58601498-58601517 Msa0338340:CDS 30.0%
!! ATAGTAACAGAATTTGGTGT+TGG - chr2_4:58601601-58601620 None:intergenic 30.0%
CAAGATTTGCAGAAATATCT+TGG - chr2_4:58601645-58601664 None:intergenic 30.0%
ATTTCTGCAAATCTTGTAGA+AGG + chr2_4:58601649-58601668 Msa0338340:intron 30.0%
AATGAGGGAGATTGTTAAAT+GGG - chr2_4:58601803-58601822 None:intergenic 30.0%
! ATTTTGCATTAGCATTAGCT+AGG + chr2_4:58601847-58601866 Msa0338340:CDS 30.0%
AATCATTCAACCATAAACCA+TGG + chr2_4:58601945-58601964 Msa0338340:CDS 30.0%
AACCATAAACCATGGATTTA+TGG + chr2_4:58601953-58601972 Msa0338340:CDS 30.0%
ATCCATAAATCCATGGTTTA+TGG - chr2_4:58601958-58601977 None:intergenic 30.0%
TAATTGCATCCATAAATCCA+TGG - chr2_4:58601965-58601984 None:intergenic 30.0%
TTTATGGATGCAATTAGAAG+TGG + chr2_4:58601969-58601988 Msa0338340:CDS 30.0%
!! AGTGGATTATTTCTTGGAAA+TGG + chr2_4:58601987-58602006 Msa0338340:CDS 30.0%
AATGCAAATAACAATGCTTG+TGG + chr2_4:58602089-58602108 Msa0338340:CDS 30.0%
ATGCAAATAACAATGCTTGT+GGG + chr2_4:58602090-58602109 Msa0338340:CDS 30.0%
!! AGTGAAAGTAAAGTTTTTGG+TGG + chr2_4:58602194-58602213 Msa0338340:CDS 30.0%
!!! GTGAAAGTAAAGTTTTTGGT+GGG + chr2_4:58602195-58602214 Msa0338340:CDS 30.0%
!!! ACGGTTCTAAATTGCAATTT+TGG + chr2_4:58602351-58602370 Msa0338340:intron 30.0%
AAATTGTGTAATTTCCGCAA+AGG - chr2_4:58602452-58602471 None:intergenic 30.0%
! GAGATTGATTTATCTGGAAT+TGG + chr2_4:58602566-58602585 Msa0338340:intron 30.0%
TAGAGGGACTAAAAAAAAGA+GGG - chr2_4:58602631-58602650 None:intergenic 30.0%
TTAGAGGGACTAAAAAAAAG+AGG - chr2_4:58602632-58602651 None:intergenic 30.0%
CAAAAAATGTGACATCTTAG+AGG - chr2_4:58602648-58602667 None:intergenic 30.0%
!!! CGACTGTCTTGTTTTAATAT+TGG + chr2_4:58602670-58602689 Msa0338340:intron 30.0%
! GAACTATTTTACCTAAAGCA+TGG + chr2_4:58602766-58602785 Msa0338340:intron 30.0%
AAACAAGAAAACCATGCTTT+AGG - chr2_4:58602780-58602799 None:intergenic 30.0%
! TCTTGTTTGATAGACAATAG+AGG + chr2_4:58602792-58602811 Msa0338340:intron 30.0%
ACAGTTAGAATCACTATTTC+AGG + chr2_4:58602878-58602897 Msa0338340:intron 30.0%
! TTCCATTACCAACCTCATTT+TGG + chr2_4:58600481-58600500 Msa0338340:five_prime_UTR 35.0%
!! ATCCAAAATGAGGTTGGTAA+TGG - chr2_4:58600486-58600505 None:intergenic 35.0%
GGGAAAAAAGAAGAAGAAAG+GGG - chr2_4:58600563-58600582 None:intergenic 35.0%
!!! AACTTTGTTTTGTGTGTGCT+TGG - chr2_4:58600599-58600618 None:intergenic 35.0%
GAAGAATATGTTAGCTAGCA+AGG - chr2_4:58600713-58600732 None:intergenic 35.0%
GCTAGCTAACATATTCTTCA+TGG + chr2_4:58600714-58600733 Msa0338340:intron 35.0%
CTAGCTTGGAAATTGAAGTT+AGG + chr2_4:58600994-58601013 Msa0338340:intron 35.0%
!! CTAGTACAAGTTTTCAAGAG+TGG + chr2_4:58601140-58601159 Msa0338340:intron 35.0%
! GCAAATCTTGTAGAAGGTAT+TGG + chr2_4:58601655-58601674 Msa0338340:intron 35.0%
! CTCTTTGAAGATGATGATGA+TGG - chr2_4:58601747-58601766 None:intergenic 35.0%
CATCATCATCATCTTCAAAG+AGG + chr2_4:58601745-58601764 Msa0338340:CDS 35.0%
!! TTGTTAAATGGGCTGAGATT+TGG - chr2_4:58601792-58601811 None:intergenic 35.0%
GAATGAGGGAGATTGTTAAA+TGG - chr2_4:58601804-58601823 None:intergenic 35.0%
AAAGCAATCATGTGGACAAA+TGG + chr2_4:58601875-58601894 Msa0338340:CDS 35.0%
AAGCAATCATGTGGACAAAT+GGG + chr2_4:58601876-58601895 Msa0338340:CDS 35.0%
GATCATGATGCTTCAAATCT+AGG + chr2_4:58601906-58601925 Msa0338340:intron 35.0%
GCTTCAAATCTAGGAAATTC+AGG + chr2_4:58601915-58601934 Msa0338340:intron 35.0%
CAGAATATGTATGGTGTGTA+TGG + chr2_4:58602032-58602051 Msa0338340:intron 35.0%
ATGTATGGTGTGTATGGAAA+TGG + chr2_4:58602038-58602057 Msa0338340:CDS 35.0%
TGTATGGTGTGTATGGAAAT+GGG + chr2_4:58602039-58602058 Msa0338340:CDS 35.0%
GACAATGGTGAAGTAAATAG+TGG + chr2_4:58602062-58602081 Msa0338340:CDS 35.0%
TGCAAATAACAATGCTTGTG+GGG + chr2_4:58602091-58602110 Msa0338340:CDS 35.0%
CTAAATGGAGGAACAAACAT+GGG + chr2_4:58602230-58602249 Msa0338340:CDS 35.0%
GTGAAACCATTATTCCAACT+TGG - chr2_4:58602275-58602294 None:intergenic 35.0%
ATAATGGTTTCACATCTCCT+TGG + chr2_4:58602282-58602301 Msa0338340:intron 35.0%
TGGTAAAATGCAGACAAATG+CGG + chr2_4:58602397-58602416 Msa0338340:intron 35.0%
!!! TCCAACTTCATTGATTTTGG+TGG - chr2_4:58602732-58602751 None:intergenic 35.0%
!!! CACTCCAACTTCATTGATTT+TGG - chr2_4:58602735-58602754 None:intergenic 35.0%
ACAAGAGAAAACTCAGAATC+AGG - chr2_4:58602854-58602873 None:intergenic 35.0%
TGCTTGGAGTGAAACTGAAT+GGG - chr2_4:58600583-58600602 None:intergenic 40.0%
AATGTCGAGCCAATCATCAA+TGG + chr2_4:58601485-58601504 Msa0338340:CDS 40.0%
! TTCAAAGCTTGTTCTGGTTG+AGG - chr2_4:58601558-58601577 None:intergenic 40.0%
!! TGTAGAAGGTATTGGACCAA+AGG + chr2_4:58601663-58601682 Msa0338340:intron 40.0%
ACCAACAGGAACATTCCTTA+GGG - chr2_4:58601694-58601713 None:intergenic 40.0%
AGGCTTCAAAAGCAATCATG+TGG + chr2_4:58601867-58601886 Msa0338340:CDS 40.0%
GTATGGTGTGTATGGAAATG+GGG + chr2_4:58602040-58602059 Msa0338340:CDS 40.0%
!!! AAGTTTTTGGTGGGTTTCCA+TGG + chr2_4:58602204-58602223 Msa0338340:CDS 40.0%
GCTAAATGGAGGAACAAACA+TGG + chr2_4:58602229-58602248 Msa0338340:CDS 40.0%
TTGAATCTGCAAGACCAAGT+TGG + chr2_4:58602258-58602277 Msa0338340:intron 40.0%
! GCAAGACCAAGTTGGAATAA+TGG + chr2_4:58602266-58602285 Msa0338340:intron 40.0%
CGTTACGACTCTTGATATTG+TGG + chr2_4:58602377-58602396 Msa0338340:intron 40.0%
! AGGCATTGTGATCGCAATTA+TGG - chr2_4:58602432-58602451 None:intergenic 40.0%
TGGTGGCAAACATACATATC+TGG - chr2_4:58602715-58602734 None:intergenic 40.0%
GCCACCAAAATCAATGAAGT+TGG + chr2_4:58602728-58602747 Msa0338340:intron 40.0%
TGGCAGAGCTGAATTTATCT+TGG - chr2_4:58602938-58602957 None:intergenic 40.0%
!! AGCAAAGGTGCTGTATAGGT+AGG - chr2_4:58600462-58600481 None:intergenic 45.0%
!! TGGAAGCAAAGGTGCTGTAT+AGG - chr2_4:58600466-58600485 None:intergenic 45.0%
!! GAGGTTGGTAATGGAAGCAA+AGG - chr2_4:58600477-58600496 None:intergenic 45.0%
GTGCTTGGAGTGAAACTGAA+TGG - chr2_4:58600584-58600603 None:intergenic 45.0%
GTTTGGATGGATCCTTCTTG+TGG + chr2_4:58600642-58600661 Msa0338340:exon 45.0%
CTAATGAGTAGTCCTACCTG+AGG - chr2_4:58600672-58600691 None:intergenic 45.0%
CTCAGGTAGGACTACTCATT+AGG + chr2_4:58600670-58600689 Msa0338340:intron 45.0%
!! GCTTGTTCTGGTTGAGGTTT+TGG - chr2_4:58601552-58601571 None:intergenic 45.0%
! GGACAGTTCAAAGCTTGTTC+TGG - chr2_4:58601564-58601583 None:intergenic 45.0%
!! GTGTTGGTTGAGTTGCATCT+TGG - chr2_4:58601585-58601604 None:intergenic 45.0%
!! AGAAGGTATTGGACCAAAGG+AGG + chr2_4:58601666-58601685 Msa0338340:intron 45.0%
ATTCCTTAGGGTTCCTCCTT+TGG - chr2_4:58601682-58601701 None:intergenic 45.0%
ACCCTAAGGAATGTTCCTGT+TGG + chr2_4:58601690-58601709 Msa0338340:CDS 45.0%
CACCAACAGGAACATTCCTT+AGG - chr2_4:58601695-58601714 None:intergenic 45.0%
CTAAGGAATGTTCCTGTTGG+TGG + chr2_4:58601693-58601712 Msa0338340:CDS 45.0%
GTGTATGGAAATGGGGACAA+TGG + chr2_4:58602047-58602066 Msa0338340:CDS 45.0%
GTGAAGCAAGAAATGTGCAG+TGG + chr2_4:58602158-58602177 Msa0338340:CDS 45.0%
AGCAAGAAATGTGCAGTGGA+AGG + chr2_4:58602162-58602181 Msa0338340:CDS 45.0%
GCAAGAAATGTGCAGTGGAA+GGG + chr2_4:58602163-58602182 Msa0338340:CDS 45.0%
TTTCCATGGCAGCTAAATGG+AGG + chr2_4:58602218-58602237 Msa0338340:CDS 45.0%
GTTTCACATCTCCTTGGCAA+GGG + chr2_4:58602288-58602307 Msa0338340:intron 45.0%
TTGCGATCACAATGCCTTTG+CGG + chr2_4:58602435-58602454 Msa0338340:intron 45.0%
TCCTTCTTGTGGACAACCTC+AGG + chr2_4:58600653-58600672 Msa0338340:CDS 50.0%
ACCTGAGGTTGTCCACAAGA+AGG - chr2_4:58600657-58600676 None:intergenic 50.0%
TCTTGTGGACAACCTCAGGT+AGG + chr2_4:58600657-58600676 Msa0338340:intron 50.0%
! AGGAATGTTCCTGTTGGTGG+TGG + chr2_4:58601696-58601715 Msa0338340:CDS 50.0%
TTTCTGCATCCACCACCAAC+AGG - chr2_4:58601708-58601727 None:intergenic 50.0%
GGGTTTCCATGGCAGCTAAA+TGG + chr2_4:58602215-58602234 Msa0338340:CDS 50.0%
GTTCCTCCATTTAGCTGCCA+TGG - chr2_4:58602224-58602243 None:intergenic 50.0%
GGTTTCACATCTCCTTGGCA+AGG + chr2_4:58602287-58602306 Msa0338340:intron 50.0%
CTATGAAGAAGCCCTTGCCA+AGG - chr2_4:58602302-58602321 None:intergenic 50.0%
GGACCAAAGGAGGAACCCTA+AGG + chr2_4:58601676-58601695 Msa0338340:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 58600432 58602969 58600432 ID=Msa0338340;Name=Msa0338340
chr2_4 mRNA 58600432 58602969 58600432 ID=Msa0338340-mRNA-1;Parent=Msa0338340;Name=Msa0338340-mRNA-1;_AED=0.19;_eAED=0.19;_QI=216|0.16|0.14|0.85|0.16|0.28|7|0|258
chr2_4 exon 58600432 58600674 58600432 ID=Msa0338340-mRNA-1:exon:18087;Parent=Msa0338340-mRNA-1
chr2_4 exon 58601483 58601630 58601483 ID=Msa0338340-mRNA-1:exon:18088;Parent=Msa0338340-mRNA-1
chr2_4 exon 58601642 58601663 58601642 ID=Msa0338340-mRNA-1:exon:18089;Parent=Msa0338340-mRNA-1
chr2_4 exon 58601671 58601897 58601671 ID=Msa0338340-mRNA-1:exon:18090;Parent=Msa0338340-mRNA-1
chr2_4 exon 58601937 58602028 58601937 ID=Msa0338340-mRNA-1:exon:18091;Parent=Msa0338340-mRNA-1
chr2_4 exon 58602035 58602275 58602035 ID=Msa0338340-mRNA-1:exon:18092;Parent=Msa0338340-mRNA-1
chr2_4 exon 58602950 58602969 58602950 ID=Msa0338340-mRNA-1:exon:18093;Parent=Msa0338340-mRNA-1
chr2_4 five_prime_UTR 58600432 58600647 58600432 ID=Msa0338340-mRNA-1:five_prime_utr;Parent=Msa0338340-mRNA-1
chr2_4 CDS 58600648 58600674 58600648 ID=Msa0338340-mRNA-1:cds;Parent=Msa0338340-mRNA-1
chr2_4 CDS 58601483 58601630 58601483 ID=Msa0338340-mRNA-1:cds;Parent=Msa0338340-mRNA-1
chr2_4 CDS 58601642 58601663 58601642 ID=Msa0338340-mRNA-1:cds;Parent=Msa0338340-mRNA-1
chr2_4 CDS 58601671 58601897 58601671 ID=Msa0338340-mRNA-1:cds;Parent=Msa0338340-mRNA-1
chr2_4 CDS 58601937 58602028 58601937 ID=Msa0338340-mRNA-1:cds;Parent=Msa0338340-mRNA-1
chr2_4 CDS 58602035 58602275 58602035 ID=Msa0338340-mRNA-1:cds;Parent=Msa0338340-mRNA-1
chr2_4 CDS 58602950 58602969 58602950 ID=Msa0338340-mRNA-1:cds;Parent=Msa0338340-mRNA-1
Gene Sequence

>Msa0338340

ATGGATCCTTCTTGTGGACAACCTCAGCAAATGTCGAGCCAATCATCAATGGAAAACATGTTAGGTTGCTCAAAAGATGAGCAAGAAAGAAAGCCAAAACCTCAACCAGAACAAGCTTTGAACTGTCCAAGATGCAACTCAACCAACACCAAATTCTGTTACTATAACAACTATACCAAGATATTTCTGCAAATCTTGTATTGGACCAAAGGAGGAACCCTAAGGAATGTTCCTGTTGGTGGTGGATGCAGAAAAAACAAAAGATCATCACCATCATCATCATCTTCAAAGAGGATTCAAGATCAAACATTCACACCAAATCTCAGCCCATTTAACAATCTCCCTCATTCATCTTATGATCATAATTCCAATGATTTTGCATTAGCATTAGCTAGGCTTCAAAAGCAATCATGTGGACAAATGGGTATGAACAATCATTCAACCATAAACCATGGATTTATGGATGCAATTAGAAGTGGATTATTTCTTGGAAATGGACTACATTATAATAATAATAATATGTATGGTGTGTATGGAAATGGGGACAATGGTGAAGTAAATAGTGGTAACAATGCAAATAACAATGCTTGTGGGGTTAGTGAAGAAATGATGTTTCCCTATGATCAAGAAATTAACAATGTGAAGCAAGAAATGTGCAGTGGAAGGGAACAAATTAGTGAAAGTAAAGTTTTTGGTGGGTTTCCATGGCAGCTAAATGGAGGAACAAACATGGGTGAAGTTGAATCTGCAAGACCAACTCTGCCAGCCATTAGATAA
Protein sequence

>Msa0338340

MDPSCGQPQQMSSQSSMENMLGCSKDEQERKPKPQPEQALNCPRCNSTNTKFCYYNNYTKIFLQILYWTKGGTLRNVPVGGGCRKNKRSSPSSSSSKRIQDQTFTPNLSPFNNLPHSSYDHNSNDFALALARLQKQSCGQMGMNNHSTINHGFMDAIRSGLFLGNGLHYNNNNMYGVYGNGDNGEVNSGNNANNNACGVSEEMMFPYDQEINNVKQEMCSGREQISESKVFGGFPWQLNGGTNMGEVESARPTLPAIR*