AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0267340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267340 MtrunA17_Chr7g0258971 93.182 264 10 2 1 261 1 259 8.69e-179 491
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267340 AT2G41950.1 53.333 195 84 1 73 260 71 265 7.94e-70 216

Find 57 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGAGAATTCAAGGGTTT+TGG 0.256792 2_2:+68920877 Msa0267340:CDS
AAGAACATCCTCAAGTTTAA+AGG 0.259636 2_2:-68921107 None:intergenic
ATCCTTTCGAACACGCAATT+TGG 0.268681 2_2:+68922752 Msa0267340:three_prime_UTR
AATTTGGTTGGACTTTAATA+TGG 0.269394 2_2:+68922768 Msa0267340:three_prime_UTR
AGGTCAGAAATTACTACTTC+TGG 0.282494 2_2:-68922605 None:intergenic
TGAGGATGTTCTTGCTTCAT+TGG 0.285239 2_2:+68921117 Msa0267340:CDS
AGTTCAGGGAAATGGAATTA+AGG 0.301525 2_2:-68920673 None:intergenic
CTTTGATGTGGCTGAATTTG+TGG 0.337260 2_2:+68922670 Msa0267340:CDS
TTGTGGTTTGATTTGAAAAC+GGG 0.341226 2_2:-68920633 None:intergenic
TTCATGGTTGAAGAAATAAC+AGG 0.383817 2_2:-68920554 None:intergenic
ATTGACCGTATTATATCATC+TGG 0.387173 2_2:+68920848 Msa0267340:CDS
TGGTCAGTGCTTGACTATAC+TGG 0.395973 2_2:+68920818 Msa0267340:CDS
AAGAATTCGTCGACAAAGAT+TGG 0.401000 2_2:+68920798 Msa0267340:CDS
GAGTTTGTGGATTGTTTAGT+TGG 0.406284 2_2:+68920917 Msa0267340:CDS
AACTAACCAGGTGAACATTT+CGG 0.410159 2_2:-68921142 None:intergenic
TGGTGGAATTTGCATGGTGG+TGG 0.415264 2_2:-68920608 None:intergenic
TTTAATTTGATTAGTATCTC+AGG 0.419037 2_2:+68922847 Msa0267340:three_prime_UTR
AAGTGGTTCTGAAGAGTTTG+TGG 0.419196 2_2:+68920904 Msa0267340:CDS
GGAAAATTGATGAGAATTCA+AGG 0.435740 2_2:+68920870 Msa0267340:CDS
GATATAATACGGTCAATGTT+TGG 0.441601 2_2:-68920842 None:intergenic
TGCTTTGCCCTTTAAACTTG+AGG 0.443940 2_2:+68921099 Msa0267340:CDS
AGAACATCCTCAAGTTTAAA+GGG 0.444786 2_2:-68921106 None:intergenic
AGCTAAAGAAGATGAATTCA+TGG 0.452626 2_2:-68920570 None:intergenic
GAGATTTGATGATGATTTGA+GGG 0.454324 2_2:-68920726 None:intergenic
GGGTGGTGGAAAATGGGTTG+TGG 0.455723 2_2:-68920650 None:intergenic
TAAAGAAACACAACATCAAA+AGG 0.463883 2_2:-68921067 None:intergenic
GTTCAGGGAAATGGAATTAA+GGG 0.464943 2_2:-68920672 None:intergenic
TTCAGTATTTCTCTACCTTG+TGG 0.465559 2_2:-68922563 None:intergenic
GGTCAGAAATTACTACTTCT+GGG 0.473415 2_2:-68922604 None:intergenic
GTTGTGGTTTGATTTGAAAA+CGG 0.484213 2_2:-68920634 None:intergenic
ATCTCGTATCCGGGCTTCAC+AGG 0.484408 2_2:-68922901 None:intergenic
TTCACAGGTTACCATGCAAA+TGG 0.486151 2_2:-68922886 None:intergenic
TCAAGTTTAAAGGGCAAAGC+AGG 0.503741 2_2:-68921097 None:intergenic
TGAGATTTGATGATGATTTG+AGG 0.510757 2_2:-68920727 None:intergenic
GGGTGGTGGAATTTGCATGG+TGG 0.512751 2_2:-68920611 None:intergenic
AGCTGCTAATTCCTTTGATG+TGG 0.512960 2_2:+68922658 Msa0267340:CDS
TTTCGAACACGCAATTTGGT+TGG 0.524335 2_2:+68922756 Msa0267340:three_prime_UTR
AACCAAATTGCGTGTTCGAA+AGG 0.526692 2_2:-68922754 None:intergenic
GATAATATTAAGTGTTGGCA+AGG 0.531345 2_2:+68921013 Msa0267340:CDS
TTTGTGGATTGTTTAGTTGG+TGG 0.542847 2_2:+68920920 Msa0267340:CDS
TTTGAGGGTTTGAAGTGAAG+TGG 0.548659 2_2:-68920711 None:intergenic
GAAAATTGATGAGAATTCAA+GGG 0.549018 2_2:+68920871 Msa0267340:CDS
TTTCTTCAGGGATTGAAACT+GGG 0.564494 2_2:-68920754 None:intergenic
CACAAATTCAGCCACATCAA+AGG 0.572163 2_2:-68922669 None:intergenic
TCGTGTAAAATCCATTTGCA+TGG 0.572284 2_2:+68922875 Msa0267340:three_prime_UTR
AATATGATAATATTAAGTGT+TGG 0.576851 2_2:+68921008 Msa0267340:CDS
TGGTGGTGGTGTGAGAATGA+AGG 0.583844 2_2:-68920594 None:intergenic
TATTAAGTGTTGGCAAGGAG+AGG 0.585342 2_2:+68921018 Msa0267340:CDS
TTGGCTCCGAAATGTTCACC+TGG 0.591346 2_2:+68921136 Msa0267340:CDS
GCTGAATTTGTGGATGAACA+TGG 0.600819 2_2:+68922680 Msa0267340:CDS
TGTGGTTTGATTTGAAAACG+GGG 0.613747 2_2:-68920632 None:intergenic
TTCAGGGAAATGGAATTAAG+GGG 0.628365 2_2:-68920671 None:intergenic
TCATGGTTGAAGAAATAACA+GGG 0.648096 2_2:-68920553 None:intergenic
GTGGTTTGATTTGAAAACGG+GGG 0.648806 2_2:-68920631 None:intergenic
TTGACCGTATTATATCATCT+GGG 0.656909 2_2:+68920849 Msa0267340:CDS
TCAGGGAAATGGAATTAAGG+GGG 0.657147 2_2:-68920670 None:intergenic
GTGATTATCAGTCATCCACA+AGG 0.692175 2_2:+68922548 Msa0267340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGATAATATTAAGTGT+TGG + chr2_2:68921008-68921027 Msa0267340:CDS 15.0%
!! TATTGAAATTGAACAATATT+TGG + chr2_2:68921412-68921431 Msa0267340:intron 15.0%
!! ATTGAAATTGAACAATATTT+GGG + chr2_2:68921413-68921432 Msa0267340:intron 15.0%
!!! CGATTTATTCTTTTTAATTT+TGG + chr2_2:68922504-68922523 Msa0267340:intron 15.0%
!! AATATGATAATATTAAGTGT+TGG + chr2_2:68921008-68921027 Msa0267340:CDS 15.0%
!! TATTGAAATTGAACAATATT+TGG + chr2_2:68921412-68921431 Msa0267340:intron 15.0%
!! ATTGAAATTGAACAATATTT+GGG + chr2_2:68921413-68921432 Msa0267340:intron 15.0%
!!! CGATTTATTCTTTTTAATTT+TGG + chr2_2:68922504-68922523 Msa0267340:intron 15.0%
!! TTACTATTGTTCCTAAAAAA+TGG + chr2_2:68921041-68921060 Msa0267340:CDS 20.0%
!!! TTTTGTTGTTAGTTTAGTAT+TGG + chr2_2:68921186-68921205 Msa0267340:intron 20.0%
!!! GATAACATTTTGTACTAAAT+AGG + chr2_2:68921337-68921356 Msa0267340:intron 20.0%
!!! AATTTGTTCAAATTAAGCTA+TGG + chr2_2:68921536-68921555 Msa0267340:intron 20.0%
!! ACAAAAATATTGTAAAACAG+CGG - chr2_2:68921578-68921597 None:intergenic 20.0%
!!! TCAAATTCCTTTTTACTATT+AGG + chr2_2:68921663-68921682 Msa0267340:intron 20.0%
!! AACTGACATAATATATAAAC+TGG - chr2_2:68921689-68921708 None:intergenic 20.0%
!! GTTTATATATTATGTCAGTT+TGG + chr2_2:68921689-68921708 Msa0267340:intron 20.0%
!!! TTTTTCTAATTTGTGATGAT+AGG - chr2_2:68921896-68921915 None:intergenic 20.0%
!!! TTTAATTTGATTAGTATCTC+AGG + chr2_2:68922847-68922866 Msa0267340:three_prime_UTR 20.0%
!! TTACTATTGTTCCTAAAAAA+TGG + chr2_2:68921041-68921060 Msa0267340:CDS 20.0%
!!! TTTTGTTGTTAGTTTAGTAT+TGG + chr2_2:68921186-68921205 Msa0267340:intron 20.0%
!!! GATAACATTTTGTACTAAAT+AGG + chr2_2:68921337-68921356 Msa0267340:intron 20.0%
!!! AATTTGTTCAAATTAAGCTA+TGG + chr2_2:68921536-68921555 Msa0267340:intron 20.0%
!! ACAAAAATATTGTAAAACAG+CGG - chr2_2:68921578-68921597 None:intergenic 20.0%
!!! TCAAATTCCTTTTTACTATT+AGG + chr2_2:68921663-68921682 Msa0267340:intron 20.0%
!! AACTGACATAATATATAAAC+TGG - chr2_2:68921689-68921708 None:intergenic 20.0%
!! GTTTATATATTATGTCAGTT+TGG + chr2_2:68921689-68921708 Msa0267340:intron 20.0%
!!! TTTTTCTAATTTGTGATGAT+AGG - chr2_2:68921896-68921915 None:intergenic 20.0%
!!! TTTAATTTGATTAGTATCTC+AGG + chr2_2:68922847-68922866 Msa0267340:three_prime_UTR 20.0%
!!! AAAATTGGATTTTGAGTTCA+GGG - chr2_2:68920690-68920709 None:intergenic 25.0%
!!! AAAAATTGGATTTTGAGTTC+AGG - chr2_2:68920691-68920710 None:intergenic 25.0%
!! TTTTCCCAGATGATATAATA+CGG - chr2_2:68920856-68920875 None:intergenic 25.0%
! GAAAATTGATGAGAATTCAA+GGG + chr2_2:68920871-68920890 Msa0267340:CDS 25.0%
! TAAAGAAACACAACATCAAA+AGG - chr2_2:68921070-68921089 None:intergenic 25.0%
! AGTTTCAATATGCAATGAAA+TGG - chr2_2:68921241-68921260 None:intergenic 25.0%
! TGAATATATGTGTTGTCAAT+TGG + chr2_2:68921496-68921515 Msa0267340:intron 25.0%
! TAAGATCTCATGAAATTAAC+AGG - chr2_2:68921803-68921822 None:intergenic 25.0%
!!! TTTTTAAAACAGAGTCCAAA+AGG + chr2_2:68922075-68922094 Msa0267340:intron 25.0%
!!! TTTTAAAACAGAGTCCAAAA+GGG + chr2_2:68922076-68922095 Msa0267340:intron 25.0%
!! TGAAACAAGAAATTGTTGTT+TGG + chr2_2:68922263-68922282 Msa0267340:intron 25.0%
! ACCTTATACATTTGATTATG+AGG + chr2_2:68922330-68922349 Msa0267340:intron 25.0%
! ATCTAGCAATGAAAAATGTA+TGG - chr2_2:68922381-68922400 None:intergenic 25.0%
!!! TAATTTTGGTTTTGACATGT+AGG + chr2_2:68922518-68922537 Msa0267340:intron 25.0%
!! CTTTGTAAATGTGTTTTATC+AGG - chr2_2:68922632-68922651 None:intergenic 25.0%
! AATTTGGTTGGACTTTAATA+TGG + chr2_2:68922768-68922787 Msa0267340:three_prime_UTR 25.0%
!! TGATAGTTAGCTAGAATTTT+AGG + chr2_2:68922803-68922822 Msa0267340:three_prime_UTR 25.0%
!!! AAAATTGGATTTTGAGTTCA+GGG - chr2_2:68920690-68920709 None:intergenic 25.0%
!!! AAAAATTGGATTTTGAGTTC+AGG - chr2_2:68920691-68920710 None:intergenic 25.0%
!! TTTTCCCAGATGATATAATA+CGG - chr2_2:68920856-68920875 None:intergenic 25.0%
! GAAAATTGATGAGAATTCAA+GGG + chr2_2:68920871-68920890 Msa0267340:CDS 25.0%
! TAAAGAAACACAACATCAAA+AGG - chr2_2:68921070-68921089 None:intergenic 25.0%
! AGTTTCAATATGCAATGAAA+TGG - chr2_2:68921241-68921260 None:intergenic 25.0%
! TGAATATATGTGTTGTCAAT+TGG + chr2_2:68921496-68921515 Msa0267340:intron 25.0%
! TAAGATCTCATGAAATTAAC+AGG - chr2_2:68921803-68921822 None:intergenic 25.0%
!!! TTTTTAAAACAGAGTCCAAA+AGG + chr2_2:68922075-68922094 Msa0267340:intron 25.0%
!!! TTTTAAAACAGAGTCCAAAA+GGG + chr2_2:68922076-68922095 Msa0267340:intron 25.0%
!! TGAAACAAGAAATTGTTGTT+TGG + chr2_2:68922263-68922282 Msa0267340:intron 25.0%
! ACCTTATACATTTGATTATG+AGG + chr2_2:68922330-68922349 Msa0267340:intron 25.0%
! ATCTAGCAATGAAAAATGTA+TGG - chr2_2:68922381-68922400 None:intergenic 25.0%
!!! TAATTTTGGTTTTGACATGT+AGG + chr2_2:68922518-68922537 Msa0267340:intron 25.0%
!! CTTTGTAAATGTGTTTTATC+AGG - chr2_2:68922632-68922651 None:intergenic 25.0%
! AATTTGGTTGGACTTTAATA+TGG + chr2_2:68922768-68922787 Msa0267340:three_prime_UTR 25.0%
!! TGATAGTTAGCTAGAATTTT+AGG + chr2_2:68922803-68922822 Msa0267340:three_prime_UTR 25.0%
TCATGGTTGAAGAAATAACA+GGG - chr2_2:68920556-68920575 None:intergenic 30.0%
TTCATGGTTGAAGAAATAAC+AGG - chr2_2:68920557-68920576 None:intergenic 30.0%
AGCTAAAGAAGATGAATTCA+TGG - chr2_2:68920573-68920592 None:intergenic 30.0%
!! TTGTGGTTTGATTTGAAAAC+GGG - chr2_2:68920636-68920655 None:intergenic 30.0%
!! GTTGTGGTTTGATTTGAAAA+CGG - chr2_2:68920637-68920656 None:intergenic 30.0%
TTGAAGTGAAGTGGAAAAAT+TGG - chr2_2:68920705-68920724 None:intergenic 30.0%
!! GAGATTTGATGATGATTTGA+GGG - chr2_2:68920729-68920748 None:intergenic 30.0%
!! TGAGATTTGATGATGATTTG+AGG - chr2_2:68920730-68920749 None:intergenic 30.0%
GATATAATACGGTCAATGTT+TGG - chr2_2:68920845-68920864 None:intergenic 30.0%
ATTGACCGTATTATATCATC+TGG + chr2_2:68920848-68920867 Msa0267340:CDS 30.0%
TTGACCGTATTATATCATCT+GGG + chr2_2:68920849-68920868 Msa0267340:CDS 30.0%
GGAAAATTGATGAGAATTCA+AGG + chr2_2:68920870-68920889 Msa0267340:CDS 30.0%
!! TCATGACTCACTTTTGATAT+TGG + chr2_2:68920970-68920989 Msa0267340:CDS 30.0%
!! GATAATATTAAGTGTTGGCA+AGG + chr2_2:68921013-68921032 Msa0267340:CDS 30.0%
!!! TCAAAAGGAGACCATTTTTT+AGG - chr2_2:68921055-68921074 None:intergenic 30.0%
AGAACATCCTCAAGTTTAAA+GGG - chr2_2:68921109-68921128 None:intergenic 30.0%
AAGAACATCCTCAAGTTTAA+AGG - chr2_2:68921110-68921129 None:intergenic 30.0%
ACAGATAAGAAAAACTAACC+AGG - chr2_2:68921157-68921176 None:intergenic 30.0%
ATAGCAATACGATAATGTCA+CGG - chr2_2:68921395-68921414 None:intergenic 30.0%
GAAATTGAACAATATTTGGG+TGG + chr2_2:68921416-68921435 Msa0267340:intron 30.0%
TATGTGTTGTCAATTGGATA+TGG + chr2_2:68921502-68921521 Msa0267340:intron 30.0%
! AAACTGGCCTAATAGTAAAA+AGG - chr2_2:68921673-68921692 None:intergenic 30.0%
ATCTCATGAAATTAACAGGT+AGG - chr2_2:68921799-68921818 None:intergenic 30.0%
!!! TTGGACTCTGTTTTAAAAAG+AGG - chr2_2:68922074-68922093 None:intergenic 30.0%
ATTGCATAGTGTTTCATGAA+AGG - chr2_2:68922184-68922203 None:intergenic 30.0%
! ATACTTACTGCTCAAGTTTT+AGG - chr2_2:68922234-68922253 None:intergenic 30.0%
GCCTCATAATCAAATGTATA+AGG - chr2_2:68922334-68922353 None:intergenic 30.0%
CACAAGAAAATCCATTAAGT+TGG - chr2_2:68922356-68922375 None:intergenic 30.0%
! GCTAGATTTTCATTGTGTTT+AGG + chr2_2:68922394-68922413 Msa0267340:intron 30.0%
! GTAAATGTGTTTTATCAGGA+AGG - chr2_2:68922628-68922647 None:intergenic 30.0%
!!! TAGCTGTTTTGATCAAATCT+AGG + chr2_2:68922711-68922730 Msa0267340:CDS 30.0%
TCATGGTTGAAGAAATAACA+GGG - chr2_2:68920556-68920575 None:intergenic 30.0%
TTCATGGTTGAAGAAATAAC+AGG - chr2_2:68920557-68920576 None:intergenic 30.0%
AGCTAAAGAAGATGAATTCA+TGG - chr2_2:68920573-68920592 None:intergenic 30.0%
!! TTGTGGTTTGATTTGAAAAC+GGG - chr2_2:68920636-68920655 None:intergenic 30.0%
!! GTTGTGGTTTGATTTGAAAA+CGG - chr2_2:68920637-68920656 None:intergenic 30.0%
TTGAAGTGAAGTGGAAAAAT+TGG - chr2_2:68920705-68920724 None:intergenic 30.0%
!! GAGATTTGATGATGATTTGA+GGG - chr2_2:68920729-68920748 None:intergenic 30.0%
!! TGAGATTTGATGATGATTTG+AGG - chr2_2:68920730-68920749 None:intergenic 30.0%
GATATAATACGGTCAATGTT+TGG - chr2_2:68920845-68920864 None:intergenic 30.0%
ATTGACCGTATTATATCATC+TGG + chr2_2:68920848-68920867 Msa0267340:CDS 30.0%
TTGACCGTATTATATCATCT+GGG + chr2_2:68920849-68920868 Msa0267340:CDS 30.0%
GGAAAATTGATGAGAATTCA+AGG + chr2_2:68920870-68920889 Msa0267340:CDS 30.0%
!! TCATGACTCACTTTTGATAT+TGG + chr2_2:68920970-68920989 Msa0267340:CDS 30.0%
!! GATAATATTAAGTGTTGGCA+AGG + chr2_2:68921013-68921032 Msa0267340:CDS 30.0%
!!! TCAAAAGGAGACCATTTTTT+AGG - chr2_2:68921055-68921074 None:intergenic 30.0%
AGAACATCCTCAAGTTTAAA+GGG - chr2_2:68921109-68921128 None:intergenic 30.0%
AAGAACATCCTCAAGTTTAA+AGG - chr2_2:68921110-68921129 None:intergenic 30.0%
ACAGATAAGAAAAACTAACC+AGG - chr2_2:68921157-68921176 None:intergenic 30.0%
ATAGCAATACGATAATGTCA+CGG - chr2_2:68921395-68921414 None:intergenic 30.0%
GAAATTGAACAATATTTGGG+TGG + chr2_2:68921416-68921435 Msa0267340:intron 30.0%
TATGTGTTGTCAATTGGATA+TGG + chr2_2:68921502-68921521 Msa0267340:intron 30.0%
! AAACTGGCCTAATAGTAAAA+AGG - chr2_2:68921673-68921692 None:intergenic 30.0%
ATCTCATGAAATTAACAGGT+AGG - chr2_2:68921799-68921818 None:intergenic 30.0%
!!! TTGGACTCTGTTTTAAAAAG+AGG - chr2_2:68922074-68922093 None:intergenic 30.0%
ATTGCATAGTGTTTCATGAA+AGG - chr2_2:68922184-68922203 None:intergenic 30.0%
! ATACTTACTGCTCAAGTTTT+AGG - chr2_2:68922234-68922253 None:intergenic 30.0%
GCCTCATAATCAAATGTATA+AGG - chr2_2:68922334-68922353 None:intergenic 30.0%
CACAAGAAAATCCATTAAGT+TGG - chr2_2:68922356-68922375 None:intergenic 30.0%
! GCTAGATTTTCATTGTGTTT+AGG + chr2_2:68922394-68922413 Msa0267340:intron 30.0%
! GTAAATGTGTTTTATCAGGA+AGG - chr2_2:68922628-68922647 None:intergenic 30.0%
!!! TAGCTGTTTTGATCAAATCT+AGG + chr2_2:68922711-68922730 Msa0267340:CDS 30.0%
! AGTGTGGTTTTCAAAGATGA+GGG - chr2_2:68920523-68920542 None:intergenic 35.0%
!! TGTGGTTTGATTTGAAAACG+GGG - chr2_2:68920635-68920654 None:intergenic 35.0%
TTCAGGGAAATGGAATTAAG+GGG - chr2_2:68920674-68920693 None:intergenic 35.0%
GTTCAGGGAAATGGAATTAA+GGG - chr2_2:68920675-68920694 None:intergenic 35.0%
AGTTCAGGGAAATGGAATTA+AGG - chr2_2:68920676-68920695 None:intergenic 35.0%
TTTCTTCAGGGATTGAAACT+GGG - chr2_2:68920757-68920776 None:intergenic 35.0%
! TTTTCTTCAGGGATTGAAAC+TGG - chr2_2:68920758-68920777 None:intergenic 35.0%
! CATAAGATGCGTTTTCTTCA+GGG - chr2_2:68920769-68920788 None:intergenic 35.0%
! ACATAAGATGCGTTTTCTTC+AGG - chr2_2:68920770-68920789 None:intergenic 35.0%
AAGAATTCGTCGACAAAGAT+TGG + chr2_2:68920798-68920817 Msa0267340:CDS 35.0%
! TGATGAGAATTCAAGGGTTT+TGG + chr2_2:68920877-68920896 Msa0267340:CDS 35.0%
! GAGTTTGTGGATTGTTTAGT+TGG + chr2_2:68920917-68920936 Msa0267340:CDS 35.0%
! TTTGTGGATTGTTTAGTTGG+TGG + chr2_2:68920920-68920939 Msa0267340:CDS 35.0%
AACTAACCAGGTGAACATTT+CGG - chr2_2:68921145-68921164 None:intergenic 35.0%
GGTGGTAGAGAAAGTAATAA+AGG + chr2_2:68921434-68921453 Msa0267340:intron 35.0%
TGAAATGCTCATCCAAATGT+TGG - chr2_2:68921646-68921665 None:intergenic 35.0%
CAATTTAGATTGCGACAACA+AGG - chr2_2:68922041-68922060 None:intergenic 35.0%
!!! ACTCTGTTTTAAAAAGAGGC+TGG - chr2_2:68922070-68922089 None:intergenic 35.0%
! AGAGGTATAGAAGCATATAG+AGG - chr2_2:68922298-68922317 None:intergenic 35.0%
TGATTATGAGGCCAACTTAA+TGG + chr2_2:68922342-68922361 Msa0267340:intron 35.0%
TTAGTGCGTGTTTGAAAATG+CGG - chr2_2:68922464-68922483 None:intergenic 35.0%
!!! TTTTGGTTTTGACATGTAGG+TGG + chr2_2:68922521-68922540 Msa0267340:intron 35.0%
TTCAGTATTTCTCTACCTTG+TGG - chr2_2:68922566-68922585 None:intergenic 35.0%
GGTCAGAAATTACTACTTCT+GGG - chr2_2:68922607-68922626 None:intergenic 35.0%
AGGTCAGAAATTACTACTTC+TGG - chr2_2:68922608-68922627 None:intergenic 35.0%
!! ATGCAAATGGATTTTACACG+AGG - chr2_2:68922876-68922895 None:intergenic 35.0%
TCGTGTAAAATCCATTTGCA+TGG + chr2_2:68922875-68922894 Msa0267340:three_prime_UTR 35.0%
! AGTGTGGTTTTCAAAGATGA+GGG - chr2_2:68920523-68920542 None:intergenic 35.0%
!! TGTGGTTTGATTTGAAAACG+GGG - chr2_2:68920635-68920654 None:intergenic 35.0%
TTCAGGGAAATGGAATTAAG+GGG - chr2_2:68920674-68920693 None:intergenic 35.0%
GTTCAGGGAAATGGAATTAA+GGG - chr2_2:68920675-68920694 None:intergenic 35.0%
AGTTCAGGGAAATGGAATTA+AGG - chr2_2:68920676-68920695 None:intergenic 35.0%
TTTCTTCAGGGATTGAAACT+GGG - chr2_2:68920757-68920776 None:intergenic 35.0%
! TTTTCTTCAGGGATTGAAAC+TGG - chr2_2:68920758-68920777 None:intergenic 35.0%
! CATAAGATGCGTTTTCTTCA+GGG - chr2_2:68920769-68920788 None:intergenic 35.0%
! ACATAAGATGCGTTTTCTTC+AGG - chr2_2:68920770-68920789 None:intergenic 35.0%
AAGAATTCGTCGACAAAGAT+TGG + chr2_2:68920798-68920817 Msa0267340:CDS 35.0%
! TGATGAGAATTCAAGGGTTT+TGG + chr2_2:68920877-68920896 Msa0267340:CDS 35.0%
! GAGTTTGTGGATTGTTTAGT+TGG + chr2_2:68920917-68920936 Msa0267340:CDS 35.0%
! TTTGTGGATTGTTTAGTTGG+TGG + chr2_2:68920920-68920939 Msa0267340:CDS 35.0%
AACTAACCAGGTGAACATTT+CGG - chr2_2:68921145-68921164 None:intergenic 35.0%
GGTGGTAGAGAAAGTAATAA+AGG + chr2_2:68921434-68921453 Msa0267340:intron 35.0%
TGAAATGCTCATCCAAATGT+TGG - chr2_2:68921646-68921665 None:intergenic 35.0%
CAATTTAGATTGCGACAACA+AGG - chr2_2:68922041-68922060 None:intergenic 35.0%
!!! ACTCTGTTTTAAAAAGAGGC+TGG - chr2_2:68922070-68922089 None:intergenic 35.0%
! AGAGGTATAGAAGCATATAG+AGG - chr2_2:68922298-68922317 None:intergenic 35.0%
TGATTATGAGGCCAACTTAA+TGG + chr2_2:68922342-68922361 Msa0267340:intron 35.0%
TTAGTGCGTGTTTGAAAATG+CGG - chr2_2:68922464-68922483 None:intergenic 35.0%
!!! TTTTGGTTTTGACATGTAGG+TGG + chr2_2:68922521-68922540 Msa0267340:intron 35.0%
TTCAGTATTTCTCTACCTTG+TGG - chr2_2:68922566-68922585 None:intergenic 35.0%
GGTCAGAAATTACTACTTCT+GGG - chr2_2:68922607-68922626 None:intergenic 35.0%
AGGTCAGAAATTACTACTTC+TGG - chr2_2:68922608-68922627 None:intergenic 35.0%
!! ATGCAAATGGATTTTACACG+AGG - chr2_2:68922876-68922895 None:intergenic 35.0%
TCGTGTAAAATCCATTTGCA+TGG + chr2_2:68922875-68922894 Msa0267340:three_prime_UTR 35.0%
! TGTGGTTTTCAAAGATGAGG+GGG - chr2_2:68920521-68920540 None:intergenic 40.0%
! GTGTGGTTTTCAAAGATGAG+GGG - chr2_2:68920522-68920541 None:intergenic 40.0%
! GAGTGTGGTTTTCAAAGATG+AGG - chr2_2:68920524-68920543 None:intergenic 40.0%
!! GTGGTTTGATTTGAAAACGG+GGG - chr2_2:68920634-68920653 None:intergenic 40.0%
TCAGGGAAATGGAATTAAGG+GGG - chr2_2:68920673-68920692 None:intergenic 40.0%
!!! GGATTTTGAGTTCAGGGAAA+TGG - chr2_2:68920684-68920703 None:intergenic 40.0%
! TTTGAGGGTTTGAAGTGAAG+TGG - chr2_2:68920714-68920733 None:intergenic 40.0%
!!! TCAAGGGTTTTGGTTTCAAG+TGG + chr2_2:68920887-68920906 Msa0267340:CDS 40.0%
!! AAGTGGTTCTGAAGAGTTTG+TGG + chr2_2:68920904-68920923 Msa0267340:CDS 40.0%
!! TATTAAGTGTTGGCAAGGAG+AGG + chr2_2:68921018-68921037 Msa0267340:CDS 40.0%
TCAAGTTTAAAGGGCAAAGC+AGG - chr2_2:68921100-68921119 None:intergenic 40.0%
TGCTTTGCCCTTTAAACTTG+AGG + chr2_2:68921099-68921118 Msa0267340:CDS 40.0%
TGAGGATGTTCTTGCTTCAT+TGG + chr2_2:68921117-68921136 Msa0267340:CDS 40.0%
CAATTGTGAATCGCAGATTG+CGG + chr2_2:68921262-68921281 Msa0267340:intron 40.0%
TCGCAGATTGCGGAAAATAA+TGG + chr2_2:68921272-68921291 Msa0267340:intron 40.0%
AAAGTAGCAGTTCTGCTTAG+CGG - chr2_2:68921317-68921336 None:intergenic 40.0%
GTGTTGTCAATTGGATATGG+CGG + chr2_2:68921505-68921524 Msa0267340:intron 40.0%
GTTCAAATTCCGCCAACATT+TGG + chr2_2:68921631-68921650 Msa0267340:intron 40.0%
AATGCTCATCCAAATGTTGG+CGG - chr2_2:68921643-68921662 None:intergenic 40.0%
! CAACTAAGAGTCTAAGACAG+TGG - chr2_2:68921941-68921960 None:intergenic 40.0%
CTTGATATTGTAGAGAGACG+CGG + chr2_2:68921971-68921990 Msa0267340:intron 40.0%
! TTTTATTGTCTCCACTCTCC+AGG - chr2_2:68922018-68922037 None:intergenic 40.0%
!!! CTGTTTTAAAAAGAGGCTGG+CGG - chr2_2:68922067-68922086 None:intergenic 40.0%
! TCATACACAATGAGCCCTTT+TGG - chr2_2:68922093-68922112 None:intergenic 40.0%
TAAGGTGCACTTTGTAGAAG+AGG - chr2_2:68922316-68922335 None:intergenic 40.0%
!! GTTTTGACATGTAGGTGGAA+GGG + chr2_2:68922526-68922545 Msa0267340:intron 40.0%
GTGATTATCAGTCATCCACA+AGG + chr2_2:68922548-68922567 Msa0267340:CDS 40.0%
AGCTGCTAATTCCTTTGATG+TGG + chr2_2:68922658-68922677 Msa0267340:CDS 40.0%
CACAAATTCAGCCACATCAA+AGG - chr2_2:68922672-68922691 None:intergenic 40.0%
CTTTGATGTGGCTGAATTTG+TGG + chr2_2:68922670-68922689 Msa0267340:CDS 40.0%
GCTGAATTTGTGGATGAACA+TGG + chr2_2:68922680-68922699 Msa0267340:CDS 40.0%
ATCCTTTCGAACACGCAATT+TGG + chr2_2:68922752-68922771 Msa0267340:three_prime_UTR 40.0%
AACCAAATTGCGTGTTCGAA+AGG - chr2_2:68922757-68922776 None:intergenic 40.0%
TTTCGAACACGCAATTTGGT+TGG + chr2_2:68922756-68922775 Msa0267340:three_prime_UTR 40.0%
TTCACAGGTTACCATGCAAA+TGG - chr2_2:68922889-68922908 None:intergenic 40.0%
! TGTGGTTTTCAAAGATGAGG+GGG - chr2_2:68920521-68920540 None:intergenic 40.0%
! GTGTGGTTTTCAAAGATGAG+GGG - chr2_2:68920522-68920541 None:intergenic 40.0%
! GAGTGTGGTTTTCAAAGATG+AGG - chr2_2:68920524-68920543 None:intergenic 40.0%
!! GTGGTTTGATTTGAAAACGG+GGG - chr2_2:68920634-68920653 None:intergenic 40.0%
TCAGGGAAATGGAATTAAGG+GGG - chr2_2:68920673-68920692 None:intergenic 40.0%
!!! GGATTTTGAGTTCAGGGAAA+TGG - chr2_2:68920684-68920703 None:intergenic 40.0%
! TTTGAGGGTTTGAAGTGAAG+TGG - chr2_2:68920714-68920733 None:intergenic 40.0%
!!! TCAAGGGTTTTGGTTTCAAG+TGG + chr2_2:68920887-68920906 Msa0267340:CDS 40.0%
!! AAGTGGTTCTGAAGAGTTTG+TGG + chr2_2:68920904-68920923 Msa0267340:CDS 40.0%
!! TATTAAGTGTTGGCAAGGAG+AGG + chr2_2:68921018-68921037 Msa0267340:CDS 40.0%
TCAAGTTTAAAGGGCAAAGC+AGG - chr2_2:68921100-68921119 None:intergenic 40.0%
TGCTTTGCCCTTTAAACTTG+AGG + chr2_2:68921099-68921118 Msa0267340:CDS 40.0%
TGAGGATGTTCTTGCTTCAT+TGG + chr2_2:68921117-68921136 Msa0267340:CDS 40.0%
CAATTGTGAATCGCAGATTG+CGG + chr2_2:68921262-68921281 Msa0267340:intron 40.0%
TCGCAGATTGCGGAAAATAA+TGG + chr2_2:68921272-68921291 Msa0267340:intron 40.0%
AAAGTAGCAGTTCTGCTTAG+CGG - chr2_2:68921317-68921336 None:intergenic 40.0%
GTGTTGTCAATTGGATATGG+CGG + chr2_2:68921505-68921524 Msa0267340:intron 40.0%
GTTCAAATTCCGCCAACATT+TGG + chr2_2:68921631-68921650 Msa0267340:intron 40.0%
AATGCTCATCCAAATGTTGG+CGG - chr2_2:68921643-68921662 None:intergenic 40.0%
! CAACTAAGAGTCTAAGACAG+TGG - chr2_2:68921941-68921960 None:intergenic 40.0%
CTTGATATTGTAGAGAGACG+CGG + chr2_2:68921971-68921990 Msa0267340:intron 40.0%
! TTTTATTGTCTCCACTCTCC+AGG - chr2_2:68922018-68922037 None:intergenic 40.0%
!!! CTGTTTTAAAAAGAGGCTGG+CGG - chr2_2:68922067-68922086 None:intergenic 40.0%
! TCATACACAATGAGCCCTTT+TGG - chr2_2:68922093-68922112 None:intergenic 40.0%
TAAGGTGCACTTTGTAGAAG+AGG - chr2_2:68922316-68922335 None:intergenic 40.0%
!! GTTTTGACATGTAGGTGGAA+GGG + chr2_2:68922526-68922545 Msa0267340:intron 40.0%
GTGATTATCAGTCATCCACA+AGG + chr2_2:68922548-68922567 Msa0267340:CDS 40.0%
AGCTGCTAATTCCTTTGATG+TGG + chr2_2:68922658-68922677 Msa0267340:CDS 40.0%
CACAAATTCAGCCACATCAA+AGG - chr2_2:68922672-68922691 None:intergenic 40.0%
CTTTGATGTGGCTGAATTTG+TGG + chr2_2:68922670-68922689 Msa0267340:CDS 40.0%
GCTGAATTTGTGGATGAACA+TGG + chr2_2:68922680-68922699 Msa0267340:CDS 40.0%
ATCCTTTCGAACACGCAATT+TGG + chr2_2:68922752-68922771 Msa0267340:three_prime_UTR 40.0%
AACCAAATTGCGTGTTCGAA+AGG - chr2_2:68922757-68922776 None:intergenic 40.0%
TTTCGAACACGCAATTTGGT+TGG + chr2_2:68922756-68922775 Msa0267340:three_prime_UTR 40.0%
TTCACAGGTTACCATGCAAA+TGG - chr2_2:68922889-68922908 None:intergenic 40.0%
! TGGTTTTCAAAGATGAGGGG+GGG - chr2_2:68920519-68920538 None:intergenic 45.0%
! GTGGTTTTCAAAGATGAGGG+GGG - chr2_2:68920520-68920539 None:intergenic 45.0%
!!! ACAGGGTTTTAGAGTGAGTG+TGG - chr2_2:68920539-68920558 None:intergenic 45.0%
!! GTTTGATTTGAAAACGGGGG+TGG - chr2_2:68920631-68920650 None:intergenic 45.0%
!! TTAAGGGGGTGGTGGAAAAT+GGG - chr2_2:68920659-68920678 None:intergenic 45.0%
!! ATTAAGGGGGTGGTGGAAAA+TGG - chr2_2:68920660-68920679 None:intergenic 45.0%
AAATGGAATTAAGGGGGTGG+TGG - chr2_2:68920667-68920686 None:intergenic 45.0%
TGGTCAGTGCTTGACTATAC+TGG + chr2_2:68920818-68920837 Msa0267340:CDS 45.0%
!! GGTTTTGACATGTAGGTGGA+AGG + chr2_2:68922525-68922544 Msa0267340:intron 45.0%
! TGGTTTTCAAAGATGAGGGG+GGG - chr2_2:68920519-68920538 None:intergenic 45.0%
! GTGGTTTTCAAAGATGAGGG+GGG - chr2_2:68920520-68920539 None:intergenic 45.0%
!!! ACAGGGTTTTAGAGTGAGTG+TGG - chr2_2:68920539-68920558 None:intergenic 45.0%
!! GTTTGATTTGAAAACGGGGG+TGG - chr2_2:68920631-68920650 None:intergenic 45.0%
!! TTAAGGGGGTGGTGGAAAAT+GGG - chr2_2:68920659-68920678 None:intergenic 45.0%
!! ATTAAGGGGGTGGTGGAAAA+TGG - chr2_2:68920660-68920679 None:intergenic 45.0%
AAATGGAATTAAGGGGGTGG+TGG - chr2_2:68920667-68920686 None:intergenic 45.0%
TGGTCAGTGCTTGACTATAC+TGG + chr2_2:68920818-68920837 Msa0267340:CDS 45.0%
!! GGTTTTGACATGTAGGTGGA+AGG + chr2_2:68922525-68922544 Msa0267340:intron 45.0%
TTTCAAAGATGAGGGGGGGT+CGG - chr2_2:68920515-68920534 None:intergenic 50.0%
!! TGGTGGTGGTGTGAGAATGA+AGG - chr2_2:68920597-68920616 None:intergenic 50.0%
TGGTGGAATTTGCATGGTGG+TGG - chr2_2:68920611-68920630 None:intergenic 50.0%
TGATTTGAAAACGGGGGTGG+TGG - chr2_2:68920628-68920647 None:intergenic 50.0%
GGGAAATGGAATTAAGGGGG+TGG - chr2_2:68920670-68920689 None:intergenic 50.0%
TTGGCTCCGAAATGTTCACC+TGG + chr2_2:68921136-68921155 Msa0267340:CDS 50.0%
ATGTGCCTCGATAGTGATCC+TGG + chr2_2:68921997-68922016 Msa0267340:intron 50.0%
ACTCTCCAGGATCACTATCG+AGG - chr2_2:68922005-68922024 None:intergenic 50.0%
TCGATAGTGATCCTGGAGAG+TGG + chr2_2:68922004-68922023 Msa0267340:intron 50.0%
GTGCGTGTTTGAAAATGCGG+TGG - chr2_2:68922461-68922480 None:intergenic 50.0%
TTTCAAAGATGAGGGGGGGT+CGG - chr2_2:68920515-68920534 None:intergenic 50.0%
!! TGGTGGTGGTGTGAGAATGA+AGG - chr2_2:68920597-68920616 None:intergenic 50.0%
TGGTGGAATTTGCATGGTGG+TGG - chr2_2:68920611-68920630 None:intergenic 50.0%
TGATTTGAAAACGGGGGTGG+TGG - chr2_2:68920628-68920647 None:intergenic 50.0%
GGGAAATGGAATTAAGGGGG+TGG - chr2_2:68920670-68920689 None:intergenic 50.0%
TTGGCTCCGAAATGTTCACC+TGG + chr2_2:68921136-68921155 Msa0267340:CDS 50.0%
ATGTGCCTCGATAGTGATCC+TGG + chr2_2:68921997-68922016 Msa0267340:intron 50.0%
ACTCTCCAGGATCACTATCG+AGG - chr2_2:68922005-68922024 None:intergenic 50.0%
TCGATAGTGATCCTGGAGAG+TGG + chr2_2:68922004-68922023 Msa0267340:intron 50.0%
GTGCGTGTTTGAAAATGCGG+TGG - chr2_2:68922461-68922480 None:intergenic 50.0%
TTCAAAGATGAGGGGGGGTC+GGG - chr2_2:68920514-68920533 None:intergenic 55.0%
!! GGGTGGTGGAATTTGCATGG+TGG - chr2_2:68920614-68920633 None:intergenic 55.0%
!! GGGTGGTGGAAAATGGGTTG+TGG - chr2_2:68920653-68920672 None:intergenic 55.0%
TTCAAAGATGAGGGGGGGTC+GGG - chr2_2:68920514-68920533 None:intergenic 55.0%
!! GGGTGGTGGAATTTGCATGG+TGG - chr2_2:68920614-68920633 None:intergenic 55.0%
!! GGGTGGTGGAAAATGGGTTG+TGG - chr2_2:68920653-68920672 None:intergenic 55.0%
!! CGGGGGTGGTGGAATTTGCA+TGG - chr2_2:68920617-68920636 None:intergenic 60.0%
!! CGGGGGTGGTGGAATTTGCA+TGG - chr2_2:68920617-68920636 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 68920473 68922908 68920473 ID=Msa0267340;Name=Msa0267340
chr2_2 mRNA 68920473 68922908 68920473 ID=Msa0267340-mRNA-1;Parent=Msa0267340;Name=Msa0267340-mRNA-1;_AED=0.00;_eAED=0.00;_QI=99|1|1|1|1|1|2|169|261
chr2_2 exon 68920473 68921157 68920473 ID=Msa0267340-mRNA-1:exon:21883;Parent=Msa0267340-mRNA-1
chr2_2 exon 68922540 68922908 68922540 ID=Msa0267340-mRNA-1:exon:21884;Parent=Msa0267340-mRNA-1
chr2_2 five_prime_UTR 68920473 68920571 68920473 ID=Msa0267340-mRNA-1:five_prime_utr;Parent=Msa0267340-mRNA-1
chr2_2 CDS 68920572 68921157 68920572 ID=Msa0267340-mRNA-1:cds;Parent=Msa0267340-mRNA-1
chr2_2 CDS 68922540 68922739 68922540 ID=Msa0267340-mRNA-1:cds;Parent=Msa0267340-mRNA-1
chr2_2 three_prime_UTR 68922740 68922908 68922740 ID=Msa0267340-mRNA-1:three_prime_utr;Parent=Msa0267340-mRNA-1
Gene Sequence

>Msa0267340

ATGAATTCATCTTCTTTAGCTTCCTTCATTCTCACACCACCACCATGCAAATTCCACCACCCCCGTTTTCAAATCAAACCACAACCCATTTTCCACCACCCCCTTAATTCCATTTCCCTGAACTCAAAATCCAATTTTTCCACTTCACTTCAAACCCTCAAATCATCATCAAATCTCAATAACCCAGTTTCAATCCCTGAAGAAAACGCATCTTATGTCGCTGTTGAAGAATTCGTCGACAAAGATTGGTCAGTGCTTGACTATACTGGACCAAACATTGACCGTATTATATCATCTGGGAAAATTGATGAGAATTCAAGGGTTTTGGTTTCAAGTGGTTCTGAAGAGTTTGTGGATTGTTTAGTTGGTGGTTCTAAATTCAAGTCTATGCTTGTTCTTCATGACTCACTTTTGATATTGGCTCTTCTTATTGAGAAATATGATAATATTAAGTGTTGGCAAGGAGAGGTTACTATTGTTCCTAAAAAATGGTCTCCTTTTGATGTTGTGTTTCTTTATTTTTTGCCTGCTTTGCCCTTTAAACTTGAGGATGTTCTTGCTTCATTGGCTCCGAAATGTTCACCTGGTGGAAGGGTGATTATCAGTCATCCACAAGGTAGAGAAATACTGAAACAGCAACGACAACAGTACCCAGAAGTAGTAATTTCTGACCTTCCTGATAAAACACATTTACAAAGTGTTGCAGCTGCTAATTCCTTTGATGTGGCTGAATTTGTGGATGAACATGGCTTTTATTTAGCTGTTTTGATCAAATCTAGGACTTAA
Protein sequence

>Msa0267340

MNSSSLASFILTPPPCKFHHPRFQIKPQPIFHHPLNSISLNSKSNFSTSLQTLKSSSNLNNPVSIPEENASYVAVEEFVDKDWSVLDYTGPNIDRIISSGKIDENSRVLVSSGSEEFVDCLVGGSKFKSMLVLHDSLLILALLIEKYDNIKCWQGEVTIVPKKWSPFDVVFLYFLPALPFKLEDVLASLAPKCSPGGRVIISHPQGREILKQQRQQYPEVVISDLPDKTHLQSVAAANSFDVAEFVDEHGFYLAVLIKSRT*