AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1163050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163050 MtrunA17_Chr7g0258971 93.182 264 10 2 1 261 1 259 8.69e-179 491
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163050 AT2G41950.1 53.333 195 84 1 73 260 71 265 7.94e-70 216

Find 57 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGAGAATTCAAGGGTTT+TGG 0.256792 7_4:+54112763 Msa1163050:CDS
AAGAACATCCTCAAGTTTAA+AGG 0.259636 7_4:-54112993 None:intergenic
ATCCTTTCGAACACGCAATT+TGG 0.268681 7_4:+54114638 Msa1163050:three_prime_UTR
AATTTGGTTGGACTTTAATA+TGG 0.269394 7_4:+54114654 Msa1163050:three_prime_UTR
AGGTCAGAAATTACTACTTC+TGG 0.282494 7_4:-54114491 None:intergenic
TGAGGATGTTCTTGCTTCAT+TGG 0.285239 7_4:+54113003 Msa1163050:CDS
AGTTCAGGGAAATGGAATTA+AGG 0.301525 7_4:-54112559 None:intergenic
CTTTGATGTGGCTGAATTTG+TGG 0.337260 7_4:+54114556 Msa1163050:CDS
TTGTGGTTTGATTTGAAAAC+GGG 0.341226 7_4:-54112519 None:intergenic
TTCATGGTTGAAGAAATAAC+AGG 0.383817 7_4:-54112440 None:intergenic
ATTGACCGTATTATATCATC+TGG 0.387173 7_4:+54112734 Msa1163050:CDS
TGGTCAGTGCTTGACTATAC+TGG 0.395973 7_4:+54112704 Msa1163050:CDS
AAGAATTCGTCGACAAAGAT+TGG 0.401000 7_4:+54112684 Msa1163050:CDS
GAGTTTGTGGATTGTTTAGT+TGG 0.406284 7_4:+54112803 Msa1163050:CDS
AACTAACCAGGTGAACATTT+CGG 0.410159 7_4:-54113028 None:intergenic
TGGTGGAATTTGCATGGTGG+TGG 0.415264 7_4:-54112494 None:intergenic
TTTAATTTGATTAGTATCTC+AGG 0.419037 7_4:+54114733 Msa1163050:three_prime_UTR
AAGTGGTTCTGAAGAGTTTG+TGG 0.419196 7_4:+54112790 Msa1163050:CDS
GGAAAATTGATGAGAATTCA+AGG 0.435740 7_4:+54112756 Msa1163050:CDS
GATATAATACGGTCAATGTT+TGG 0.441601 7_4:-54112728 None:intergenic
TGCTTTGCCCTTTAAACTTG+AGG 0.443940 7_4:+54112985 Msa1163050:CDS
AGAACATCCTCAAGTTTAAA+GGG 0.444786 7_4:-54112992 None:intergenic
AGCTAAAGAAGATGAATTCA+TGG 0.452626 7_4:-54112456 None:intergenic
GAGATTTGATGATGATTTGA+GGG 0.454324 7_4:-54112612 None:intergenic
GGGTGGTGGAAAATGGGTTG+TGG 0.455723 7_4:-54112536 None:intergenic
TAAAGAAACACAACATCAAA+AGG 0.463883 7_4:-54112953 None:intergenic
GTTCAGGGAAATGGAATTAA+GGG 0.464943 7_4:-54112558 None:intergenic
TTCAGTATTTCTCTACCTTG+TGG 0.465559 7_4:-54114449 None:intergenic
GGTCAGAAATTACTACTTCT+GGG 0.473415 7_4:-54114490 None:intergenic
GTTGTGGTTTGATTTGAAAA+CGG 0.484213 7_4:-54112520 None:intergenic
ATCTCGTATCCGGGCTTCAC+AGG 0.484408 7_4:-54114787 None:intergenic
TTCACAGGTTACCATGCAAA+TGG 0.486151 7_4:-54114772 None:intergenic
TCAAGTTTAAAGGGCAAAGC+AGG 0.503741 7_4:-54112983 None:intergenic
TGAGATTTGATGATGATTTG+AGG 0.510757 7_4:-54112613 None:intergenic
GGGTGGTGGAATTTGCATGG+TGG 0.512751 7_4:-54112497 None:intergenic
AGCTGCTAATTCCTTTGATG+TGG 0.512960 7_4:+54114544 Msa1163050:CDS
TTTCGAACACGCAATTTGGT+TGG 0.524335 7_4:+54114642 Msa1163050:three_prime_UTR
AACCAAATTGCGTGTTCGAA+AGG 0.526692 7_4:-54114640 None:intergenic
GATAATATTAAGTGTTGGCA+AGG 0.531345 7_4:+54112899 Msa1163050:CDS
TTTGTGGATTGTTTAGTTGG+TGG 0.542847 7_4:+54112806 Msa1163050:CDS
TTTGAGGGTTTGAAGTGAAG+TGG 0.548659 7_4:-54112597 None:intergenic
GAAAATTGATGAGAATTCAA+GGG 0.549018 7_4:+54112757 Msa1163050:CDS
TTTCTTCAGGGATTGAAACT+GGG 0.564494 7_4:-54112640 None:intergenic
CACAAATTCAGCCACATCAA+AGG 0.572163 7_4:-54114555 None:intergenic
TCGTGTAAAATCCATTTGCA+TGG 0.572284 7_4:+54114761 Msa1163050:three_prime_UTR
AATATGATAATATTAAGTGT+TGG 0.576851 7_4:+54112894 Msa1163050:CDS
TGGTGGTGGTGTGAGAATGA+AGG 0.583844 7_4:-54112480 None:intergenic
TATTAAGTGTTGGCAAGGAG+AGG 0.585342 7_4:+54112904 Msa1163050:CDS
TTGGCTCCGAAATGTTCACC+TGG 0.591346 7_4:+54113022 Msa1163050:CDS
GCTGAATTTGTGGATGAACA+TGG 0.600819 7_4:+54114566 Msa1163050:CDS
TGTGGTTTGATTTGAAAACG+GGG 0.613747 7_4:-54112518 None:intergenic
TTCAGGGAAATGGAATTAAG+GGG 0.628365 7_4:-54112557 None:intergenic
TCATGGTTGAAGAAATAACA+GGG 0.648096 7_4:-54112439 None:intergenic
GTGGTTTGATTTGAAAACGG+GGG 0.648806 7_4:-54112517 None:intergenic
TTGACCGTATTATATCATCT+GGG 0.656909 7_4:+54112735 Msa1163050:CDS
TCAGGGAAATGGAATTAAGG+GGG 0.657147 7_4:-54112556 None:intergenic
GTGATTATCAGTCATCCACA+AGG 0.692175 7_4:+54114434 Msa1163050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGATAATATTAAGTGT+TGG + chr7_4:54112894-54112913 Msa1163050:CDS 15.0%
!! TATTGAAATTGAACAATATT+TGG + chr7_4:54113298-54113317 Msa1163050:intron 15.0%
!! ATTGAAATTGAACAATATTT+GGG + chr7_4:54113299-54113318 Msa1163050:intron 15.0%
!!! CGATTTATTCTTTTTAATTT+TGG + chr7_4:54114390-54114409 Msa1163050:intron 15.0%
!! AATATGATAATATTAAGTGT+TGG + chr7_4:54112894-54112913 Msa1163050:CDS 15.0%
!! TATTGAAATTGAACAATATT+TGG + chr7_4:54113298-54113317 Msa1163050:intron 15.0%
!! ATTGAAATTGAACAATATTT+GGG + chr7_4:54113299-54113318 Msa1163050:intron 15.0%
!!! CGATTTATTCTTTTTAATTT+TGG + chr7_4:54114390-54114409 Msa1163050:intron 15.0%
!! TTACTATTGTTCCTAAAAAA+TGG + chr7_4:54112927-54112946 Msa1163050:CDS 20.0%
!!! TTTTGTTGTTAGTTTAGTAT+TGG + chr7_4:54113072-54113091 Msa1163050:intron 20.0%
!!! GATAACATTTTGTACTAAAT+AGG + chr7_4:54113223-54113242 Msa1163050:intron 20.0%
!!! AATTTGTTCAAATTAAGCTA+TGG + chr7_4:54113422-54113441 Msa1163050:intron 20.0%
!! ACAAAAATATTGTAAAACAG+CGG - chr7_4:54113464-54113483 None:intergenic 20.0%
!!! TCAAATTCCTTTTTACTATT+AGG + chr7_4:54113549-54113568 Msa1163050:intron 20.0%
!! AACTGACATAATATATAAAC+TGG - chr7_4:54113575-54113594 None:intergenic 20.0%
!! GTTTATATATTATGTCAGTT+TGG + chr7_4:54113575-54113594 Msa1163050:intron 20.0%
!!! TTTTTCTAATTTGTGATGAT+AGG - chr7_4:54113782-54113801 None:intergenic 20.0%
!!! TTTAATTTGATTAGTATCTC+AGG + chr7_4:54114733-54114752 Msa1163050:three_prime_UTR 20.0%
!! TTACTATTGTTCCTAAAAAA+TGG + chr7_4:54112927-54112946 Msa1163050:CDS 20.0%
!!! TTTTGTTGTTAGTTTAGTAT+TGG + chr7_4:54113072-54113091 Msa1163050:intron 20.0%
!!! GATAACATTTTGTACTAAAT+AGG + chr7_4:54113223-54113242 Msa1163050:intron 20.0%
!!! AATTTGTTCAAATTAAGCTA+TGG + chr7_4:54113422-54113441 Msa1163050:intron 20.0%
!! ACAAAAATATTGTAAAACAG+CGG - chr7_4:54113464-54113483 None:intergenic 20.0%
!!! TCAAATTCCTTTTTACTATT+AGG + chr7_4:54113549-54113568 Msa1163050:intron 20.0%
!! AACTGACATAATATATAAAC+TGG - chr7_4:54113575-54113594 None:intergenic 20.0%
!! GTTTATATATTATGTCAGTT+TGG + chr7_4:54113575-54113594 Msa1163050:intron 20.0%
!!! TTTTTCTAATTTGTGATGAT+AGG - chr7_4:54113782-54113801 None:intergenic 20.0%
!!! TTTAATTTGATTAGTATCTC+AGG + chr7_4:54114733-54114752 Msa1163050:three_prime_UTR 20.0%
!!! AAAATTGGATTTTGAGTTCA+GGG - chr7_4:54112576-54112595 None:intergenic 25.0%
!!! AAAAATTGGATTTTGAGTTC+AGG - chr7_4:54112577-54112596 None:intergenic 25.0%
!! TTTTCCCAGATGATATAATA+CGG - chr7_4:54112742-54112761 None:intergenic 25.0%
! GAAAATTGATGAGAATTCAA+GGG + chr7_4:54112757-54112776 Msa1163050:CDS 25.0%
! TAAAGAAACACAACATCAAA+AGG - chr7_4:54112956-54112975 None:intergenic 25.0%
! AGTTTCAATATGCAATGAAA+TGG - chr7_4:54113127-54113146 None:intergenic 25.0%
! TGAATATATGTGTTGTCAAT+TGG + chr7_4:54113382-54113401 Msa1163050:intron 25.0%
! TAAGATCTCATGAAATTAAC+AGG - chr7_4:54113689-54113708 None:intergenic 25.0%
!!! TTTTTAAAACAGAGTCCAAA+AGG + chr7_4:54113961-54113980 Msa1163050:intron 25.0%
!!! TTTTAAAACAGAGTCCAAAA+GGG + chr7_4:54113962-54113981 Msa1163050:intron 25.0%
!! TGAAACAAGAAATTGTTGTT+TGG + chr7_4:54114149-54114168 Msa1163050:intron 25.0%
! ACCTTATACATTTGATTATG+AGG + chr7_4:54114216-54114235 Msa1163050:intron 25.0%
! ATCTAGCAATGAAAAATGTA+TGG - chr7_4:54114267-54114286 None:intergenic 25.0%
!!! TAATTTTGGTTTTGACATGT+AGG + chr7_4:54114404-54114423 Msa1163050:intron 25.0%
!! CTTTGTAAATGTGTTTTATC+AGG - chr7_4:54114518-54114537 None:intergenic 25.0%
! AATTTGGTTGGACTTTAATA+TGG + chr7_4:54114654-54114673 Msa1163050:three_prime_UTR 25.0%
!! TGATAGTTAGCTAGAATTTT+AGG + chr7_4:54114689-54114708 Msa1163050:three_prime_UTR 25.0%
!!! AAAATTGGATTTTGAGTTCA+GGG - chr7_4:54112576-54112595 None:intergenic 25.0%
!!! AAAAATTGGATTTTGAGTTC+AGG - chr7_4:54112577-54112596 None:intergenic 25.0%
!! TTTTCCCAGATGATATAATA+CGG - chr7_4:54112742-54112761 None:intergenic 25.0%
! GAAAATTGATGAGAATTCAA+GGG + chr7_4:54112757-54112776 Msa1163050:CDS 25.0%
! TAAAGAAACACAACATCAAA+AGG - chr7_4:54112956-54112975 None:intergenic 25.0%
! AGTTTCAATATGCAATGAAA+TGG - chr7_4:54113127-54113146 None:intergenic 25.0%
! TGAATATATGTGTTGTCAAT+TGG + chr7_4:54113382-54113401 Msa1163050:intron 25.0%
! TAAGATCTCATGAAATTAAC+AGG - chr7_4:54113689-54113708 None:intergenic 25.0%
!!! TTTTTAAAACAGAGTCCAAA+AGG + chr7_4:54113961-54113980 Msa1163050:intron 25.0%
!!! TTTTAAAACAGAGTCCAAAA+GGG + chr7_4:54113962-54113981 Msa1163050:intron 25.0%
!! TGAAACAAGAAATTGTTGTT+TGG + chr7_4:54114149-54114168 Msa1163050:intron 25.0%
! ACCTTATACATTTGATTATG+AGG + chr7_4:54114216-54114235 Msa1163050:intron 25.0%
! ATCTAGCAATGAAAAATGTA+TGG - chr7_4:54114267-54114286 None:intergenic 25.0%
!!! TAATTTTGGTTTTGACATGT+AGG + chr7_4:54114404-54114423 Msa1163050:intron 25.0%
!! CTTTGTAAATGTGTTTTATC+AGG - chr7_4:54114518-54114537 None:intergenic 25.0%
! AATTTGGTTGGACTTTAATA+TGG + chr7_4:54114654-54114673 Msa1163050:three_prime_UTR 25.0%
!! TGATAGTTAGCTAGAATTTT+AGG + chr7_4:54114689-54114708 Msa1163050:three_prime_UTR 25.0%
TCATGGTTGAAGAAATAACA+GGG - chr7_4:54112442-54112461 None:intergenic 30.0%
TTCATGGTTGAAGAAATAAC+AGG - chr7_4:54112443-54112462 None:intergenic 30.0%
AGCTAAAGAAGATGAATTCA+TGG - chr7_4:54112459-54112478 None:intergenic 30.0%
!! TTGTGGTTTGATTTGAAAAC+GGG - chr7_4:54112522-54112541 None:intergenic 30.0%
!! GTTGTGGTTTGATTTGAAAA+CGG - chr7_4:54112523-54112542 None:intergenic 30.0%
TTGAAGTGAAGTGGAAAAAT+TGG - chr7_4:54112591-54112610 None:intergenic 30.0%
!! GAGATTTGATGATGATTTGA+GGG - chr7_4:54112615-54112634 None:intergenic 30.0%
!! TGAGATTTGATGATGATTTG+AGG - chr7_4:54112616-54112635 None:intergenic 30.0%
GATATAATACGGTCAATGTT+TGG - chr7_4:54112731-54112750 None:intergenic 30.0%
ATTGACCGTATTATATCATC+TGG + chr7_4:54112734-54112753 Msa1163050:CDS 30.0%
TTGACCGTATTATATCATCT+GGG + chr7_4:54112735-54112754 Msa1163050:CDS 30.0%
GGAAAATTGATGAGAATTCA+AGG + chr7_4:54112756-54112775 Msa1163050:CDS 30.0%
!! TCATGACTCACTTTTGATAT+TGG + chr7_4:54112856-54112875 Msa1163050:CDS 30.0%
!! GATAATATTAAGTGTTGGCA+AGG + chr7_4:54112899-54112918 Msa1163050:CDS 30.0%
!!! TCAAAAGGAGACCATTTTTT+AGG - chr7_4:54112941-54112960 None:intergenic 30.0%
AGAACATCCTCAAGTTTAAA+GGG - chr7_4:54112995-54113014 None:intergenic 30.0%
AAGAACATCCTCAAGTTTAA+AGG - chr7_4:54112996-54113015 None:intergenic 30.0%
ACAGATAAGAAAAACTAACC+AGG - chr7_4:54113043-54113062 None:intergenic 30.0%
ATAGCAATACGATAATGTCA+CGG - chr7_4:54113281-54113300 None:intergenic 30.0%
GAAATTGAACAATATTTGGG+TGG + chr7_4:54113302-54113321 Msa1163050:intron 30.0%
TATGTGTTGTCAATTGGATA+TGG + chr7_4:54113388-54113407 Msa1163050:intron 30.0%
! AAACTGGCCTAATAGTAAAA+AGG - chr7_4:54113559-54113578 None:intergenic 30.0%
ATCTCATGAAATTAACAGGT+AGG - chr7_4:54113685-54113704 None:intergenic 30.0%
!!! TTGGACTCTGTTTTAAAAAG+AGG - chr7_4:54113960-54113979 None:intergenic 30.0%
ATTGCATAGTGTTTCATGAA+AGG - chr7_4:54114070-54114089 None:intergenic 30.0%
! ATACTTACTGCTCAAGTTTT+AGG - chr7_4:54114120-54114139 None:intergenic 30.0%
GCCTCATAATCAAATGTATA+AGG - chr7_4:54114220-54114239 None:intergenic 30.0%
CACAAGAAAATCCATTAAGT+TGG - chr7_4:54114242-54114261 None:intergenic 30.0%
! GCTAGATTTTCATTGTGTTT+AGG + chr7_4:54114280-54114299 Msa1163050:intron 30.0%
! GTAAATGTGTTTTATCAGGA+AGG - chr7_4:54114514-54114533 None:intergenic 30.0%
!!! TAGCTGTTTTGATCAAATCT+AGG + chr7_4:54114597-54114616 Msa1163050:CDS 30.0%
TCATGGTTGAAGAAATAACA+GGG - chr7_4:54112442-54112461 None:intergenic 30.0%
TTCATGGTTGAAGAAATAAC+AGG - chr7_4:54112443-54112462 None:intergenic 30.0%
AGCTAAAGAAGATGAATTCA+TGG - chr7_4:54112459-54112478 None:intergenic 30.0%
!! TTGTGGTTTGATTTGAAAAC+GGG - chr7_4:54112522-54112541 None:intergenic 30.0%
!! GTTGTGGTTTGATTTGAAAA+CGG - chr7_4:54112523-54112542 None:intergenic 30.0%
TTGAAGTGAAGTGGAAAAAT+TGG - chr7_4:54112591-54112610 None:intergenic 30.0%
!! GAGATTTGATGATGATTTGA+GGG - chr7_4:54112615-54112634 None:intergenic 30.0%
!! TGAGATTTGATGATGATTTG+AGG - chr7_4:54112616-54112635 None:intergenic 30.0%
GATATAATACGGTCAATGTT+TGG - chr7_4:54112731-54112750 None:intergenic 30.0%
ATTGACCGTATTATATCATC+TGG + chr7_4:54112734-54112753 Msa1163050:CDS 30.0%
TTGACCGTATTATATCATCT+GGG + chr7_4:54112735-54112754 Msa1163050:CDS 30.0%
GGAAAATTGATGAGAATTCA+AGG + chr7_4:54112756-54112775 Msa1163050:CDS 30.0%
!! TCATGACTCACTTTTGATAT+TGG + chr7_4:54112856-54112875 Msa1163050:CDS 30.0%
!! GATAATATTAAGTGTTGGCA+AGG + chr7_4:54112899-54112918 Msa1163050:CDS 30.0%
!!! TCAAAAGGAGACCATTTTTT+AGG - chr7_4:54112941-54112960 None:intergenic 30.0%
AGAACATCCTCAAGTTTAAA+GGG - chr7_4:54112995-54113014 None:intergenic 30.0%
AAGAACATCCTCAAGTTTAA+AGG - chr7_4:54112996-54113015 None:intergenic 30.0%
ACAGATAAGAAAAACTAACC+AGG - chr7_4:54113043-54113062 None:intergenic 30.0%
ATAGCAATACGATAATGTCA+CGG - chr7_4:54113281-54113300 None:intergenic 30.0%
GAAATTGAACAATATTTGGG+TGG + chr7_4:54113302-54113321 Msa1163050:intron 30.0%
TATGTGTTGTCAATTGGATA+TGG + chr7_4:54113388-54113407 Msa1163050:intron 30.0%
! AAACTGGCCTAATAGTAAAA+AGG - chr7_4:54113559-54113578 None:intergenic 30.0%
ATCTCATGAAATTAACAGGT+AGG - chr7_4:54113685-54113704 None:intergenic 30.0%
!!! TTGGACTCTGTTTTAAAAAG+AGG - chr7_4:54113960-54113979 None:intergenic 30.0%
ATTGCATAGTGTTTCATGAA+AGG - chr7_4:54114070-54114089 None:intergenic 30.0%
! ATACTTACTGCTCAAGTTTT+AGG - chr7_4:54114120-54114139 None:intergenic 30.0%
GCCTCATAATCAAATGTATA+AGG - chr7_4:54114220-54114239 None:intergenic 30.0%
CACAAGAAAATCCATTAAGT+TGG - chr7_4:54114242-54114261 None:intergenic 30.0%
! GCTAGATTTTCATTGTGTTT+AGG + chr7_4:54114280-54114299 Msa1163050:intron 30.0%
! GTAAATGTGTTTTATCAGGA+AGG - chr7_4:54114514-54114533 None:intergenic 30.0%
!!! TAGCTGTTTTGATCAAATCT+AGG + chr7_4:54114597-54114616 Msa1163050:CDS 30.0%
! AGTGTGGTTTTCAAAGATGA+GGG - chr7_4:54112409-54112428 None:intergenic 35.0%
!! TGTGGTTTGATTTGAAAACG+GGG - chr7_4:54112521-54112540 None:intergenic 35.0%
TTCAGGGAAATGGAATTAAG+GGG - chr7_4:54112560-54112579 None:intergenic 35.0%
GTTCAGGGAAATGGAATTAA+GGG - chr7_4:54112561-54112580 None:intergenic 35.0%
AGTTCAGGGAAATGGAATTA+AGG - chr7_4:54112562-54112581 None:intergenic 35.0%
TTTCTTCAGGGATTGAAACT+GGG - chr7_4:54112643-54112662 None:intergenic 35.0%
! TTTTCTTCAGGGATTGAAAC+TGG - chr7_4:54112644-54112663 None:intergenic 35.0%
! CATAAGATGCGTTTTCTTCA+GGG - chr7_4:54112655-54112674 None:intergenic 35.0%
! ACATAAGATGCGTTTTCTTC+AGG - chr7_4:54112656-54112675 None:intergenic 35.0%
AAGAATTCGTCGACAAAGAT+TGG + chr7_4:54112684-54112703 Msa1163050:CDS 35.0%
! TGATGAGAATTCAAGGGTTT+TGG + chr7_4:54112763-54112782 Msa1163050:CDS 35.0%
! GAGTTTGTGGATTGTTTAGT+TGG + chr7_4:54112803-54112822 Msa1163050:CDS 35.0%
! TTTGTGGATTGTTTAGTTGG+TGG + chr7_4:54112806-54112825 Msa1163050:CDS 35.0%
AACTAACCAGGTGAACATTT+CGG - chr7_4:54113031-54113050 None:intergenic 35.0%
GGTGGTAGAGAAAGTAATAA+AGG + chr7_4:54113320-54113339 Msa1163050:intron 35.0%
TGAAATGCTCATCCAAATGT+TGG - chr7_4:54113532-54113551 None:intergenic 35.0%
CAATTTAGATTGCGACAACA+AGG - chr7_4:54113927-54113946 None:intergenic 35.0%
!!! ACTCTGTTTTAAAAAGAGGC+TGG - chr7_4:54113956-54113975 None:intergenic 35.0%
! AGAGGTATAGAAGCATATAG+AGG - chr7_4:54114184-54114203 None:intergenic 35.0%
TGATTATGAGGCCAACTTAA+TGG + chr7_4:54114228-54114247 Msa1163050:intron 35.0%
TTAGTGCGTGTTTGAAAATG+CGG - chr7_4:54114350-54114369 None:intergenic 35.0%
!!! TTTTGGTTTTGACATGTAGG+TGG + chr7_4:54114407-54114426 Msa1163050:intron 35.0%
TTCAGTATTTCTCTACCTTG+TGG - chr7_4:54114452-54114471 None:intergenic 35.0%
GGTCAGAAATTACTACTTCT+GGG - chr7_4:54114493-54114512 None:intergenic 35.0%
AGGTCAGAAATTACTACTTC+TGG - chr7_4:54114494-54114513 None:intergenic 35.0%
!! ATGCAAATGGATTTTACACG+AGG - chr7_4:54114762-54114781 None:intergenic 35.0%
TCGTGTAAAATCCATTTGCA+TGG + chr7_4:54114761-54114780 Msa1163050:three_prime_UTR 35.0%
! AGTGTGGTTTTCAAAGATGA+GGG - chr7_4:54112409-54112428 None:intergenic 35.0%
!! TGTGGTTTGATTTGAAAACG+GGG - chr7_4:54112521-54112540 None:intergenic 35.0%
TTCAGGGAAATGGAATTAAG+GGG - chr7_4:54112560-54112579 None:intergenic 35.0%
GTTCAGGGAAATGGAATTAA+GGG - chr7_4:54112561-54112580 None:intergenic 35.0%
AGTTCAGGGAAATGGAATTA+AGG - chr7_4:54112562-54112581 None:intergenic 35.0%
TTTCTTCAGGGATTGAAACT+GGG - chr7_4:54112643-54112662 None:intergenic 35.0%
! TTTTCTTCAGGGATTGAAAC+TGG - chr7_4:54112644-54112663 None:intergenic 35.0%
! CATAAGATGCGTTTTCTTCA+GGG - chr7_4:54112655-54112674 None:intergenic 35.0%
! ACATAAGATGCGTTTTCTTC+AGG - chr7_4:54112656-54112675 None:intergenic 35.0%
AAGAATTCGTCGACAAAGAT+TGG + chr7_4:54112684-54112703 Msa1163050:CDS 35.0%
! TGATGAGAATTCAAGGGTTT+TGG + chr7_4:54112763-54112782 Msa1163050:CDS 35.0%
! GAGTTTGTGGATTGTTTAGT+TGG + chr7_4:54112803-54112822 Msa1163050:CDS 35.0%
! TTTGTGGATTGTTTAGTTGG+TGG + chr7_4:54112806-54112825 Msa1163050:CDS 35.0%
AACTAACCAGGTGAACATTT+CGG - chr7_4:54113031-54113050 None:intergenic 35.0%
GGTGGTAGAGAAAGTAATAA+AGG + chr7_4:54113320-54113339 Msa1163050:intron 35.0%
TGAAATGCTCATCCAAATGT+TGG - chr7_4:54113532-54113551 None:intergenic 35.0%
CAATTTAGATTGCGACAACA+AGG - chr7_4:54113927-54113946 None:intergenic 35.0%
!!! ACTCTGTTTTAAAAAGAGGC+TGG - chr7_4:54113956-54113975 None:intergenic 35.0%
! AGAGGTATAGAAGCATATAG+AGG - chr7_4:54114184-54114203 None:intergenic 35.0%
TGATTATGAGGCCAACTTAA+TGG + chr7_4:54114228-54114247 Msa1163050:intron 35.0%
TTAGTGCGTGTTTGAAAATG+CGG - chr7_4:54114350-54114369 None:intergenic 35.0%
!!! TTTTGGTTTTGACATGTAGG+TGG + chr7_4:54114407-54114426 Msa1163050:intron 35.0%
TTCAGTATTTCTCTACCTTG+TGG - chr7_4:54114452-54114471 None:intergenic 35.0%
GGTCAGAAATTACTACTTCT+GGG - chr7_4:54114493-54114512 None:intergenic 35.0%
AGGTCAGAAATTACTACTTC+TGG - chr7_4:54114494-54114513 None:intergenic 35.0%
!! ATGCAAATGGATTTTACACG+AGG - chr7_4:54114762-54114781 None:intergenic 35.0%
TCGTGTAAAATCCATTTGCA+TGG + chr7_4:54114761-54114780 Msa1163050:three_prime_UTR 35.0%
! TGTGGTTTTCAAAGATGAGG+GGG - chr7_4:54112407-54112426 None:intergenic 40.0%
! GTGTGGTTTTCAAAGATGAG+GGG - chr7_4:54112408-54112427 None:intergenic 40.0%
! GAGTGTGGTTTTCAAAGATG+AGG - chr7_4:54112410-54112429 None:intergenic 40.0%
!! GTGGTTTGATTTGAAAACGG+GGG - chr7_4:54112520-54112539 None:intergenic 40.0%
TCAGGGAAATGGAATTAAGG+GGG - chr7_4:54112559-54112578 None:intergenic 40.0%
!!! GGATTTTGAGTTCAGGGAAA+TGG - chr7_4:54112570-54112589 None:intergenic 40.0%
! TTTGAGGGTTTGAAGTGAAG+TGG - chr7_4:54112600-54112619 None:intergenic 40.0%
!!! TCAAGGGTTTTGGTTTCAAG+TGG + chr7_4:54112773-54112792 Msa1163050:CDS 40.0%
!! AAGTGGTTCTGAAGAGTTTG+TGG + chr7_4:54112790-54112809 Msa1163050:CDS 40.0%
!! TATTAAGTGTTGGCAAGGAG+AGG + chr7_4:54112904-54112923 Msa1163050:CDS 40.0%
TCAAGTTTAAAGGGCAAAGC+AGG - chr7_4:54112986-54113005 None:intergenic 40.0%
TGCTTTGCCCTTTAAACTTG+AGG + chr7_4:54112985-54113004 Msa1163050:CDS 40.0%
TGAGGATGTTCTTGCTTCAT+TGG + chr7_4:54113003-54113022 Msa1163050:CDS 40.0%
CAATTGTGAATCGCAGATTG+CGG + chr7_4:54113148-54113167 Msa1163050:intron 40.0%
TCGCAGATTGCGGAAAATAA+TGG + chr7_4:54113158-54113177 Msa1163050:intron 40.0%
AAAGTAGCAGTTCTGCTTAG+CGG - chr7_4:54113203-54113222 None:intergenic 40.0%
GTGTTGTCAATTGGATATGG+CGG + chr7_4:54113391-54113410 Msa1163050:intron 40.0%
GTTCAAATTCCGCCAACATT+TGG + chr7_4:54113517-54113536 Msa1163050:intron 40.0%
AATGCTCATCCAAATGTTGG+CGG - chr7_4:54113529-54113548 None:intergenic 40.0%
! CAACTAAGAGTCTAAGACAG+TGG - chr7_4:54113827-54113846 None:intergenic 40.0%
CTTGATATTGTAGAGAGACG+CGG + chr7_4:54113857-54113876 Msa1163050:intron 40.0%
! TTTTATTGTCTCCACTCTCC+AGG - chr7_4:54113904-54113923 None:intergenic 40.0%
!!! CTGTTTTAAAAAGAGGCTGG+CGG - chr7_4:54113953-54113972 None:intergenic 40.0%
! TCATACACAATGAGCCCTTT+TGG - chr7_4:54113979-54113998 None:intergenic 40.0%
TAAGGTGCACTTTGTAGAAG+AGG - chr7_4:54114202-54114221 None:intergenic 40.0%
!! GTTTTGACATGTAGGTGGAA+GGG + chr7_4:54114412-54114431 Msa1163050:intron 40.0%
GTGATTATCAGTCATCCACA+AGG + chr7_4:54114434-54114453 Msa1163050:CDS 40.0%
AGCTGCTAATTCCTTTGATG+TGG + chr7_4:54114544-54114563 Msa1163050:CDS 40.0%
CACAAATTCAGCCACATCAA+AGG - chr7_4:54114558-54114577 None:intergenic 40.0%
CTTTGATGTGGCTGAATTTG+TGG + chr7_4:54114556-54114575 Msa1163050:CDS 40.0%
GCTGAATTTGTGGATGAACA+TGG + chr7_4:54114566-54114585 Msa1163050:CDS 40.0%
ATCCTTTCGAACACGCAATT+TGG + chr7_4:54114638-54114657 Msa1163050:three_prime_UTR 40.0%
AACCAAATTGCGTGTTCGAA+AGG - chr7_4:54114643-54114662 None:intergenic 40.0%
TTTCGAACACGCAATTTGGT+TGG + chr7_4:54114642-54114661 Msa1163050:three_prime_UTR 40.0%
TTCACAGGTTACCATGCAAA+TGG - chr7_4:54114775-54114794 None:intergenic 40.0%
! TGTGGTTTTCAAAGATGAGG+GGG - chr7_4:54112407-54112426 None:intergenic 40.0%
! GTGTGGTTTTCAAAGATGAG+GGG - chr7_4:54112408-54112427 None:intergenic 40.0%
! GAGTGTGGTTTTCAAAGATG+AGG - chr7_4:54112410-54112429 None:intergenic 40.0%
!! GTGGTTTGATTTGAAAACGG+GGG - chr7_4:54112520-54112539 None:intergenic 40.0%
TCAGGGAAATGGAATTAAGG+GGG - chr7_4:54112559-54112578 None:intergenic 40.0%
!!! GGATTTTGAGTTCAGGGAAA+TGG - chr7_4:54112570-54112589 None:intergenic 40.0%
! TTTGAGGGTTTGAAGTGAAG+TGG - chr7_4:54112600-54112619 None:intergenic 40.0%
!!! TCAAGGGTTTTGGTTTCAAG+TGG + chr7_4:54112773-54112792 Msa1163050:CDS 40.0%
!! AAGTGGTTCTGAAGAGTTTG+TGG + chr7_4:54112790-54112809 Msa1163050:CDS 40.0%
!! TATTAAGTGTTGGCAAGGAG+AGG + chr7_4:54112904-54112923 Msa1163050:CDS 40.0%
TCAAGTTTAAAGGGCAAAGC+AGG - chr7_4:54112986-54113005 None:intergenic 40.0%
TGCTTTGCCCTTTAAACTTG+AGG + chr7_4:54112985-54113004 Msa1163050:CDS 40.0%
TGAGGATGTTCTTGCTTCAT+TGG + chr7_4:54113003-54113022 Msa1163050:CDS 40.0%
CAATTGTGAATCGCAGATTG+CGG + chr7_4:54113148-54113167 Msa1163050:intron 40.0%
TCGCAGATTGCGGAAAATAA+TGG + chr7_4:54113158-54113177 Msa1163050:intron 40.0%
AAAGTAGCAGTTCTGCTTAG+CGG - chr7_4:54113203-54113222 None:intergenic 40.0%
GTGTTGTCAATTGGATATGG+CGG + chr7_4:54113391-54113410 Msa1163050:intron 40.0%
GTTCAAATTCCGCCAACATT+TGG + chr7_4:54113517-54113536 Msa1163050:intron 40.0%
AATGCTCATCCAAATGTTGG+CGG - chr7_4:54113529-54113548 None:intergenic 40.0%
! CAACTAAGAGTCTAAGACAG+TGG - chr7_4:54113827-54113846 None:intergenic 40.0%
CTTGATATTGTAGAGAGACG+CGG + chr7_4:54113857-54113876 Msa1163050:intron 40.0%
! TTTTATTGTCTCCACTCTCC+AGG - chr7_4:54113904-54113923 None:intergenic 40.0%
!!! CTGTTTTAAAAAGAGGCTGG+CGG - chr7_4:54113953-54113972 None:intergenic 40.0%
! TCATACACAATGAGCCCTTT+TGG - chr7_4:54113979-54113998 None:intergenic 40.0%
TAAGGTGCACTTTGTAGAAG+AGG - chr7_4:54114202-54114221 None:intergenic 40.0%
!! GTTTTGACATGTAGGTGGAA+GGG + chr7_4:54114412-54114431 Msa1163050:intron 40.0%
GTGATTATCAGTCATCCACA+AGG + chr7_4:54114434-54114453 Msa1163050:CDS 40.0%
AGCTGCTAATTCCTTTGATG+TGG + chr7_4:54114544-54114563 Msa1163050:CDS 40.0%
CACAAATTCAGCCACATCAA+AGG - chr7_4:54114558-54114577 None:intergenic 40.0%
CTTTGATGTGGCTGAATTTG+TGG + chr7_4:54114556-54114575 Msa1163050:CDS 40.0%
GCTGAATTTGTGGATGAACA+TGG + chr7_4:54114566-54114585 Msa1163050:CDS 40.0%
ATCCTTTCGAACACGCAATT+TGG + chr7_4:54114638-54114657 Msa1163050:three_prime_UTR 40.0%
AACCAAATTGCGTGTTCGAA+AGG - chr7_4:54114643-54114662 None:intergenic 40.0%
TTTCGAACACGCAATTTGGT+TGG + chr7_4:54114642-54114661 Msa1163050:three_prime_UTR 40.0%
TTCACAGGTTACCATGCAAA+TGG - chr7_4:54114775-54114794 None:intergenic 40.0%
! TGGTTTTCAAAGATGAGGGG+GGG - chr7_4:54112405-54112424 None:intergenic 45.0%
! GTGGTTTTCAAAGATGAGGG+GGG - chr7_4:54112406-54112425 None:intergenic 45.0%
!!! ACAGGGTTTTAGAGTGAGTG+TGG - chr7_4:54112425-54112444 None:intergenic 45.0%
!! GTTTGATTTGAAAACGGGGG+TGG - chr7_4:54112517-54112536 None:intergenic 45.0%
!! TTAAGGGGGTGGTGGAAAAT+GGG - chr7_4:54112545-54112564 None:intergenic 45.0%
!! ATTAAGGGGGTGGTGGAAAA+TGG - chr7_4:54112546-54112565 None:intergenic 45.0%
AAATGGAATTAAGGGGGTGG+TGG - chr7_4:54112553-54112572 None:intergenic 45.0%
TGGTCAGTGCTTGACTATAC+TGG + chr7_4:54112704-54112723 Msa1163050:CDS 45.0%
!! GGTTTTGACATGTAGGTGGA+AGG + chr7_4:54114411-54114430 Msa1163050:intron 45.0%
! TGGTTTTCAAAGATGAGGGG+GGG - chr7_4:54112405-54112424 None:intergenic 45.0%
! GTGGTTTTCAAAGATGAGGG+GGG - chr7_4:54112406-54112425 None:intergenic 45.0%
!!! ACAGGGTTTTAGAGTGAGTG+TGG - chr7_4:54112425-54112444 None:intergenic 45.0%
!! GTTTGATTTGAAAACGGGGG+TGG - chr7_4:54112517-54112536 None:intergenic 45.0%
!! TTAAGGGGGTGGTGGAAAAT+GGG - chr7_4:54112545-54112564 None:intergenic 45.0%
!! ATTAAGGGGGTGGTGGAAAA+TGG - chr7_4:54112546-54112565 None:intergenic 45.0%
AAATGGAATTAAGGGGGTGG+TGG - chr7_4:54112553-54112572 None:intergenic 45.0%
TGGTCAGTGCTTGACTATAC+TGG + chr7_4:54112704-54112723 Msa1163050:CDS 45.0%
!! GGTTTTGACATGTAGGTGGA+AGG + chr7_4:54114411-54114430 Msa1163050:intron 45.0%
TTTCAAAGATGAGGGGGGGT+CGG - chr7_4:54112401-54112420 None:intergenic 50.0%
!! TGGTGGTGGTGTGAGAATGA+AGG - chr7_4:54112483-54112502 None:intergenic 50.0%
TGGTGGAATTTGCATGGTGG+TGG - chr7_4:54112497-54112516 None:intergenic 50.0%
TGATTTGAAAACGGGGGTGG+TGG - chr7_4:54112514-54112533 None:intergenic 50.0%
GGGAAATGGAATTAAGGGGG+TGG - chr7_4:54112556-54112575 None:intergenic 50.0%
TTGGCTCCGAAATGTTCACC+TGG + chr7_4:54113022-54113041 Msa1163050:CDS 50.0%
ATGTGCCTCGATAGTGATCC+TGG + chr7_4:54113883-54113902 Msa1163050:intron 50.0%
ACTCTCCAGGATCACTATCG+AGG - chr7_4:54113891-54113910 None:intergenic 50.0%
TCGATAGTGATCCTGGAGAG+TGG + chr7_4:54113890-54113909 Msa1163050:intron 50.0%
GTGCGTGTTTGAAAATGCGG+TGG - chr7_4:54114347-54114366 None:intergenic 50.0%
TTTCAAAGATGAGGGGGGGT+CGG - chr7_4:54112401-54112420 None:intergenic 50.0%
!! TGGTGGTGGTGTGAGAATGA+AGG - chr7_4:54112483-54112502 None:intergenic 50.0%
TGGTGGAATTTGCATGGTGG+TGG - chr7_4:54112497-54112516 None:intergenic 50.0%
TGATTTGAAAACGGGGGTGG+TGG - chr7_4:54112514-54112533 None:intergenic 50.0%
GGGAAATGGAATTAAGGGGG+TGG - chr7_4:54112556-54112575 None:intergenic 50.0%
TTGGCTCCGAAATGTTCACC+TGG + chr7_4:54113022-54113041 Msa1163050:CDS 50.0%
ATGTGCCTCGATAGTGATCC+TGG + chr7_4:54113883-54113902 Msa1163050:intron 50.0%
ACTCTCCAGGATCACTATCG+AGG - chr7_4:54113891-54113910 None:intergenic 50.0%
TCGATAGTGATCCTGGAGAG+TGG + chr7_4:54113890-54113909 Msa1163050:intron 50.0%
GTGCGTGTTTGAAAATGCGG+TGG - chr7_4:54114347-54114366 None:intergenic 50.0%
TTCAAAGATGAGGGGGGGTC+GGG - chr7_4:54112400-54112419 None:intergenic 55.0%
!! GGGTGGTGGAATTTGCATGG+TGG - chr7_4:54112500-54112519 None:intergenic 55.0%
!! GGGTGGTGGAAAATGGGTTG+TGG - chr7_4:54112539-54112558 None:intergenic 55.0%
TTCAAAGATGAGGGGGGGTC+GGG - chr7_4:54112400-54112419 None:intergenic 55.0%
!! GGGTGGTGGAATTTGCATGG+TGG - chr7_4:54112500-54112519 None:intergenic 55.0%
!! GGGTGGTGGAAAATGGGTTG+TGG - chr7_4:54112539-54112558 None:intergenic 55.0%
!! CGGGGGTGGTGGAATTTGCA+TGG - chr7_4:54112503-54112522 None:intergenic 60.0%
!! CGGGGGTGGTGGAATTTGCA+TGG - chr7_4:54112503-54112522 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 54112359 54114794 54112359 ID=Msa1163050;Name=Msa1163050
chr7_4 mRNA 54112359 54114794 54112359 ID=Msa1163050-mRNA-1;Parent=Msa1163050;Name=Msa1163050-mRNA-1;_AED=0.00;_eAED=0.00;_QI=99|1|1|1|1|1|2|169|261
chr7_4 exon 54112359 54113043 54112359 ID=Msa1163050-mRNA-1:exon:15679;Parent=Msa1163050-mRNA-1
chr7_4 exon 54114426 54114794 54114426 ID=Msa1163050-mRNA-1:exon:15680;Parent=Msa1163050-mRNA-1
chr7_4 five_prime_UTR 54112359 54112457 54112359 ID=Msa1163050-mRNA-1:five_prime_utr;Parent=Msa1163050-mRNA-1
chr7_4 CDS 54112458 54113043 54112458 ID=Msa1163050-mRNA-1:cds;Parent=Msa1163050-mRNA-1
chr7_4 CDS 54114426 54114625 54114426 ID=Msa1163050-mRNA-1:cds;Parent=Msa1163050-mRNA-1
chr7_4 three_prime_UTR 54114626 54114794 54114626 ID=Msa1163050-mRNA-1:three_prime_utr;Parent=Msa1163050-mRNA-1
Gene Sequence

>Msa1163050

ATGAATTCATCTTCTTTAGCTTCCTTCATTCTCACACCACCACCATGCAAATTCCACCACCCCCGTTTTCAAATCAAACCACAACCCATTTTCCACCACCCCCTTAATTCCATTTCCCTGAACTCAAAATCCAATTTTTCCACTTCACTTCAAACCCTCAAATCATCATCAAATCTCAATAACCCAGTTTCAATCCCTGAAGAAAACGCATCTTATGTCGCTGTTGAAGAATTCGTCGACAAAGATTGGTCAGTGCTTGACTATACTGGACCAAACATTGACCGTATTATATCATCTGGGAAAATTGATGAGAATTCAAGGGTTTTGGTTTCAAGTGGTTCTGAAGAGTTTGTGGATTGTTTAGTTGGTGGTTCTAAATTCAAGTCTATGCTTGTTCTTCATGACTCACTTTTGATATTGGCTCTTCTTATTGAGAAATATGATAATATTAAGTGTTGGCAAGGAGAGGTTACTATTGTTCCTAAAAAATGGTCTCCTTTTGATGTTGTGTTTCTTTATTTTTTGCCTGCTTTGCCCTTTAAACTTGAGGATGTTCTTGCTTCATTGGCTCCGAAATGTTCACCTGGTGGAAGGGTGATTATCAGTCATCCACAAGGTAGAGAAATACTGAAACAGCAACGACAACAGTACCCAGAAGTAGTAATTTCTGACCTTCCTGATAAAACACATTTACAAAGTGTTGCAGCTGCTAATTCCTTTGATGTGGCTGAATTTGTGGATGAACATGGCTTTTATTTAGCTGTTTTGATCAAATCTAGGACTTAA
Protein sequence

>Msa1163050

MNSSSLASFILTPPPCKFHHPRFQIKPQPIFHHPLNSISLNSKSNFSTSLQTLKSSSNLNNPVSIPEENASYVAVEEFVDKDWSVLDYTGPNIDRIISSGKIDENSRVLVSSGSEEFVDCLVGGSKFKSMLVLHDSLLILALLIEKYDNIKCWQGEVTIVPKKWSPFDVVFLYFLPALPFKLEDVLASLAPKCSPGGRVIISHPQGREILKQQRQQYPEVVISDLPDKTHLQSVAAANSFDVAEFVDEHGFYLAVLIKSRT*