AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0351110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0351110 MtrunA17_Chr3g0085901 85.088 114 17 0 3 116 240 353 4.17e-59 185
Msa0351110 MtrunA17_Chr7g0249591 55.963 109 48 0 1 109 251 359 4.13e-28 105
Msa0351110 MtrunA17_Chr3g0078191 57.009 107 44 1 3 107 291 397 8.67e-28 104
Msa0351110 MtrunA17_Chr2g0331731 53.271 107 48 1 3 107 66 172 2.55e-25 94.0
Msa0351110 MtrunA17_Chr4g0070811 55.140 107 46 1 3 107 162 268 4.74e-25 95.5
Msa0351110 MtrunA17_Chr6g0457621 53.774 106 44 2 3 104 269 373 5.46e-24 94.4
Msa0351110 MtrunA17_Chr2g0325081 52.336 107 49 1 3 107 258 364 6.61e-24 94.0
Msa0351110 MtrunA17_Chr6g0460081 53.774 106 44 2 3 104 269 373 6.77e-24 94.0
Msa0351110 MtrunA17_Chr7g0221741 51.852 108 50 1 2 107 247 354 7.11e-24 93.6
Msa0351110 MtrunA17_Chr3g0082451 52.381 105 50 0 3 107 245 349 8.00e-24 93.6
Msa0351110 MtrunA17_Chr3g0082431 52.381 105 50 0 3 107 246 350 8.25e-24 93.6
Msa0351110 MtrunA17_Chr5g0424761 50.467 107 52 1 3 109 254 359 1.60e-23 92.8
Msa0351110 MtrunA17_Chr2g0331741 54.630 108 45 3 3 107 323 429 1.84e-23 93.2
Msa0351110 MtrunA17_Chr5g0427711 59.048 105 43 0 3 107 244 348 4.47e-23 91.7
Msa0351110 MtrunA17_Chr6g0457681 53.271 107 46 3 3 107 299 403 1.67e-21 87.8
Msa0351110 MtrunA17_Chr8g0364381 50.943 106 48 3 3 105 319 423 2.21e-21 87.4
Msa0351110 MtrunA17_Chr8g0366411 47.407 135 38 3 5 108 319 451 9.93e-21 85.9
Msa0351110 MtrunA17_Chr3g0078891 50.476 105 52 0 3 107 266 370 2.23e-20 84.7
Msa0351110 MtrunA17_Chr3g0077781 54.369 103 43 3 3 104 263 362 2.36e-20 84.3
Msa0351110 MtrunA17_Chr4g0076201 40.000 145 45 3 3 108 32 173 2.27e-19 79.0
Msa0351110 MtrunA17_Chr3g0078731 48.624 109 52 1 3 107 266 374 5.94e-19 80.5
Msa0351110 MtrunA17_Chr4g0049121 42.759 145 42 4 3 108 285 427 9.51e-19 80.1
Msa0351110 MtrunA17_Chr6g0486921 37.241 145 50 2 3 108 281 423 1.25e-18 79.7
Msa0351110 MtrunA17_Chr6g0486911 39.189 148 46 3 3 108 260 405 7.03e-18 77.4
Msa0351110 MtrunA17_Chr4g0049531 38.889 144 48 2 3 108 200 341 1.66e-17 76.3
Msa0351110 MtrunA17_Chr3g0085941 50.926 108 48 2 3 106 251 357 9.77e-17 74.3
Msa0351110 MtrunA17_Chr3g0099441 38.298 141 49 2 3 107 309 447 1.21e-16 74.3
Msa0351110 MtrunA17_Chr4g0049031 46.212 132 45 2 3 110 266 395 1.85e-16 73.6
Msa0351110 MtrunA17_Chr4g0049081 37.241 145 50 3 3 108 30 172 3.22e-16 70.5
Msa0351110 MtrunA17_Chr7g0221701 46.729 107 46 2 2 107 218 314 8.64e-16 71.2
Msa0351110 MtrunA17_Chr3g0084261 40.833 120 46 1 13 107 273 392 9.87e-16 71.6
Msa0351110 MtrunA17_Chr3g0091161 40.602 133 44 2 11 108 256 388 1.33e-15 71.2
Msa0351110 MtrunA17_Chr4g0048151 36.667 150 55 3 3 114 270 417 1.43e-15 71.2
Msa0351110 MtrunA17_Chr4g0049141 35.897 156 47 4 3 112 70 218 1.71e-15 70.5
Msa0351110 MtrunA17_Chr3g0085951 39.695 131 44 1 10 105 244 374 1.90e-15 70.9
Msa0351110 MtrunA17_Chr4g0049071 36.029 136 50 1 8 106 292 427 4.44e-15 69.7
Msa0351110 MtrunA17_Chr3g0084241 41.880 117 42 2 13 106 255 368 4.64e-15 69.7
Msa0351110 MtrunA17_Chr3g0085981 41.353 133 43 2 10 107 157 289 5.47e-15 68.9
Msa0351110 MtrunA17_Chr3g0085961 47.059 102 26 2 34 107 282 383 8.53e-15 68.9
Msa0351110 MtrunA17_Chr4g0005311 40.000 130 40 3 15 107 271 399 3.06e-14 67.4
Msa0351110 MtrunA17_Chr3g0132661 35.461 141 53 2 3 107 263 401 1.34e-13 65.5
Msa0351110 MtrunA17_Chr4g0049091 37.838 148 50 5 3 111 177 321 7.32e-13 63.5
Msa0351110 MtrunA17_Chr4g0049111 34.483 145 54 2 3 108 283 425 1.28e-12 62.8
Msa0351110 MtrunA17_Chr4g0049041 34.395 157 56 3 3 114 264 418 1.31e-12 62.8
Msa0351110 MtrunA17_Chr3g0084251 37.705 122 49 3 13 107 449 570 3.11e-12 61.6
Msa0351110 MtrunA17_Chr5g0404531 49.315 73 33 1 7 79 118 186 5.91e-12 59.3
Msa0351110 MtrunA17_Chr3g0091201 63.636 55 20 0 3 57 269 323 1.34e-11 59.7
Msa0351110 MtrunA17_Chr5g0419301 33.099 142 57 3 3 108 270 409 1.80e-11 59.3
Msa0351110 MtrunA17_Chr3g0077791 58.182 55 23 0 3 57 270 324 7.42e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTTCATGTAATATTAAT+TGG 0.138209 3_1:+17058166 Msa0351110:three_prime_UTR
CTATCACTTAGCTTATATTT+AGG 0.246978 3_1:+17054354 Msa0351110:five_prime_UTR
AGATAGCTCAATTTCGAAAT+AGG 0.280967 3_1:-17054604 None:intergenic
CAAACTATGCTGAAGATCTA+TGG 0.286643 3_1:+17054660 Msa0351110:exon
AGCTCAACCTTCGATAATTC+AGG 0.369068 3_1:-17055009 None:intergenic
CTTGCTTGATTATAATATAT+AGG 0.371682 3_1:-17058207 None:intergenic
GTTAATTTAACTATACTTAC+GGG 0.382839 3_1:+17054573 Msa0351110:five_prime_UTR
TTTGATCATTGATAATTGTT+TGG 0.386183 3_1:+17054506 Msa0351110:five_prime_UTR
ATAGTTAAATTAACAAGCTT+GGG 0.393579 3_1:-17054564 None:intergenic
TATTTCATGTAATATTAATT+GGG 0.408176 3_1:+17058167 Msa0351110:three_prime_UTR
TATAGTTAAATTAACAAGCT+TGG 0.411078 3_1:-17054565 None:intergenic
TTTGAGATGTGTTTACTTGT+GGG 0.427782 3_1:+17058129 Msa0351110:three_prime_UTR
CTTCGTTGATTCTACTTAGC+TGG 0.436870 3_1:+17054754 Msa0351110:CDS
TGTTAATTTAACTATACTTA+CGG 0.444111 3_1:+17054572 Msa0351110:five_prime_UTR
GTCTACTATTCGACCAGGTA+TGG 0.477241 3_1:-17055085 None:intergenic
CAGCAACTTCAAGTTATACA+AGG 0.482770 3_1:-17055037 None:intergenic
GTTGATTCTACTTAGCTGGC+TGG 0.484430 3_1:+17054758 Msa0351110:CDS
AAAGGGCTCGACACTATAGA+AGG 0.487480 3_1:-17054460 None:intergenic
AAATATAAGCTAAGTGATAG+AGG 0.490987 3_1:-17054351 None:intergenic
CATGGATGAAGCGAATGATA+AGG 0.505095 3_1:+17058087 Msa0351110:three_prime_UTR
TTGTACTAGACATAATGTAT+AGG 0.519879 3_1:-17058285 None:intergenic
ACTCTGGTCATAACTTCCTC+TGG 0.520357 3_1:+17055154 Msa0351110:CDS
CGTCTCTTTAGATGCTCTCC+AGG 0.529961 3_1:+17054809 Msa0351110:CDS
CTCAGTTCCTGAATTATCGA+AGG 0.530013 3_1:+17055002 Msa0351110:CDS
AAACTATGCTGAAGATCTAT+GGG 0.530161 3_1:+17054661 Msa0351110:exon
ATGACAAAGCAGAAGAGCTA+AGG 0.545138 3_1:+17058049 Msa0351110:three_prime_UTR
TCTCAAAATAATAAGCATCA+AGG 0.549097 3_1:-17058113 None:intergenic
GCTTGGGACTTGATATGCAA+AGG 0.559227 3_1:-17054548 None:intergenic
TTCCCACGCTCATCCATACC+TGG 0.565796 3_1:+17055072 Msa0351110:CDS
AAAGAAAAGGTACGAAGACA+TGG 0.570399 3_1:-17054630 None:intergenic
AGGAGAAAATGACGAAAACA+TGG 0.573848 3_1:+17058069 Msa0351110:three_prime_UTR
TGAAGTTGCTGAAAGTAGGA+AGG 0.579459 3_1:+17055049 Msa0351110:CDS
AGTTTGACCAGTAAAAGAAA+AGG 0.580501 3_1:-17054643 None:intergenic
AACTTCAAGTTATACAAGGA+AGG 0.591050 3_1:-17055033 None:intergenic
AAGACATGTGCGTGATTCAG+CGG 0.592722 3_1:+17054728 Msa0351110:CDS
GTTAACGTGATAACTTACTC+TGG 0.600140 3_1:+17055138 Msa0351110:CDS
TCAAAACTCACCCTCAGCAA+TGG 0.604257 3_1:+17055116 Msa0351110:CDS
TATCACGTTAACCATTGCTG+AGG 0.618338 3_1:-17055127 None:intergenic
CGACCAGGTATGGATGAGCG+TGG 0.619266 3_1:-17055075 None:intergenic
AACTTGAAGTTGCTGAAAGT+AGG 0.621828 3_1:+17055045 Msa0351110:CDS
TTACATGCTTGATAGAAGAG+AGG 0.622342 3_1:-17054695 None:intergenic
ATCACGTTAACCATTGCTGA+GGG 0.629609 3_1:-17055126 None:intergenic
CTTCGATAATTCAGGAACTG+AGG 0.634058 3_1:-17055001 None:intergenic
CTGGAGAGCATCTAAAGAGA+CGG 0.645546 3_1:-17054808 None:intergenic
GACCAGGTATGGATGAGCGT+GGG 0.649807 3_1:-17055074 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGGTAAAAATTAAAAATTAT+AGG + chr3_1:17055380-17055399 Msa0351110:intron 10.0%
!! TATTCATCAATTAAAAAAAA+AGG + chr3_1:17056231-17056250 Msa0351110:intron 10.0%
!! ATTCATCAATTAAAAAAAAA+GGG + chr3_1:17056232-17056251 Msa0351110:intron 10.0%
!!! AACTTTTAAAAAATTACATT+TGG - chr3_1:17056723-17056742 None:intergenic 10.0%
!!! AATTTTTTAAAAGTTTTCAT+TGG + chr3_1:17056728-17056747 Msa0351110:intron 10.0%
!! TATTTCATGTAATATTAATT+GGG + chr3_1:17058167-17058186 Msa0351110:three_prime_UTR 10.0%
!! TGTTAATTTAACTATACTTA+CGG + chr3_1:17054572-17054591 Msa0351110:five_prime_UTR 15.0%
!!! ATTTAAAGTGAAAAATGAAA+TGG - chr3_1:17054872-17054891 None:intergenic 15.0%
!!! CAAATATATAATTTGTTGTT+AGG - chr3_1:17055763-17055782 None:intergenic 15.0%
!! CTAACAACAAATTATATATT+TGG + chr3_1:17055761-17055780 Msa0351110:intron 15.0%
!!! CTATTTTAAAAAATCTTGAA+AGG - chr3_1:17056777-17056796 None:intergenic 15.0%
!!! GAAAATAAGGATTATTTTAA+AGG - chr3_1:17057072-17057091 None:intergenic 15.0%
!!! TATTTAAGACTCAATTAATT+AGG + chr3_1:17057332-17057351 Msa0351110:intron 15.0%
!! CTATTTCATGTAATATTAAT+TGG + chr3_1:17058166-17058185 Msa0351110:three_prime_UTR 15.0%
!!! AATATTTTAAATGCCGAAAA+GGG - chr3_1:17054480-17054499 None:intergenic 20.0%
!!! TAATATTTTAAATGCCGAAA+AGG - chr3_1:17054481-17054500 None:intergenic 20.0%
!!! TTTGATCATTGATAATTGTT+TGG + chr3_1:17054506-17054525 Msa0351110:five_prime_UTR 20.0%
!! ATAGTTAAATTAACAAGCTT+GGG - chr3_1:17054567-17054586 None:intergenic 20.0%
!! TATAGTTAAATTAACAAGCT+TGG - chr3_1:17054568-17054587 None:intergenic 20.0%
!! GTTAATTTAACTATACTTAC+GGG + chr3_1:17054573-17054592 Msa0351110:five_prime_UTR 20.0%
!!! AAGTTTGTTTATCTACATAA+GGG - chr3_1:17055330-17055349 None:intergenic 20.0%
!! AATTATAAGTTACTAGCATT+CGG - chr3_1:17055736-17055755 None:intergenic 20.0%
!!! GAATTCTTTTGTAAAAACTT+TGG - chr3_1:17055848-17055867 None:intergenic 20.0%
!!! GGTTTGATTTGATTTAATAT+GGG - chr3_1:17055968-17055987 None:intergenic 20.0%
!!! TTTTGGTAATTCAATTAGTA+CGG + chr3_1:17056100-17056119 Msa0351110:intron 20.0%
!! AAAGATTCTTAACTATTTCA+TGG - chr3_1:17056933-17056952 None:intergenic 20.0%
!! TCTTTATTAATGTTCATCAT+TGG + chr3_1:17056948-17056967 Msa0351110:intron 20.0%
!!! TAAGGATTATTTTAAAGGAA+GGG - chr3_1:17057067-17057086 None:intergenic 20.0%
!!! ATAAGGATTATTTTAAAGGA+AGG - chr3_1:17057068-17057087 None:intergenic 20.0%
!! ATATATTCATGGAAATGAAA+AGG - chr3_1:17057130-17057149 None:intergenic 20.0%
!! TTCATTTCCATGAATATATA+AGG + chr3_1:17057131-17057150 Msa0351110:intron 20.0%
!!! TTTATAGCCTTATATATTCA+TGG - chr3_1:17057141-17057160 None:intergenic 20.0%
!!! AATTTAGCTTACAAAAAAGA+TGG + chr3_1:17057168-17057187 Msa0351110:intron 20.0%
!!! AGCAAATTTTATCTTAAATC+AGG + chr3_1:17057203-17057222 Msa0351110:intron 20.0%
!! NNNNNNNNAGGCTAAAGTTT+CGG - chr3_1:17057392-17057411 None:intergenic 20.0%
!!! TGGATTTGTTTATTACTAAA+GGG - chr3_1:17057571-17057590 None:intergenic 20.0%
!!! ATGGATTTGTTTATTACTAA+AGG - chr3_1:17057572-17057591 None:intergenic 20.0%
!! GAGATTGATAGATAAAAAAA+GGG - chr3_1:17057975-17057994 None:intergenic 20.0%
!! AGAGATTGATAGATAAAAAA+AGG - chr3_1:17057976-17057995 None:intergenic 20.0%
!! CTTGCTTGATTATAATATAT+AGG - chr3_1:17058210-17058229 None:intergenic 20.0%
! AAATATAAGCTAAGTGATAG+AGG - chr3_1:17054354-17054373 None:intergenic 25.0%
! CTATCACTTAGCTTATATTT+AGG + chr3_1:17054354-17054373 Msa0351110:five_prime_UTR 25.0%
! ATAAACACAACATAAAAAGC+AGG - chr3_1:17054918-17054937 None:intergenic 25.0%
!!! TGGTGATAACTTACTTTTTA+AGG + chr3_1:17055174-17055193 Msa0351110:exon 25.0%
!!! ATAACTTACTTTTTAAGGTG+AGG + chr3_1:17055179-17055198 Msa0351110:intron 25.0%
!! CAAGTTTGTTTATCTACATA+AGG - chr3_1:17055331-17055350 None:intergenic 25.0%
! ATTATAGGTCCTAATAGTAT+TGG + chr3_1:17055395-17055414 Msa0351110:intron 25.0%
! TTATAGGTCCTAATAGTATT+GGG + chr3_1:17055396-17055415 Msa0351110:intron 25.0%
! AAGATAAAGGTAACTGAAAA+TGG + chr3_1:17055658-17055677 Msa0351110:intron 25.0%
! AGATAAAGGTAACTGAAAAT+GGG + chr3_1:17055659-17055678 Msa0351110:intron 25.0%
! TAGATTTAAACGTAACATCT+AGG - chr3_1:17055794-17055813 None:intergenic 25.0%
! GTAAAAACTTTGGTTGAAAA+GGG - chr3_1:17055838-17055857 None:intergenic 25.0%
! TGTAAAAACTTTGGTTGAAA+AGG - chr3_1:17055839-17055858 None:intergenic 25.0%
!!! GGGTTTGATTTGATTTAATA+TGG - chr3_1:17055969-17055988 None:intergenic 25.0%
!!! ATGTGTGTTTCATGTTATTT+TGG + chr3_1:17056083-17056102 Msa0351110:intron 25.0%
! AATTAGTACGGAAATTAAAG+TGG + chr3_1:17056112-17056131 Msa0351110:intron 25.0%
! ATTAGTACGGAAATTAAAGT+GGG + chr3_1:17056113-17056132 Msa0351110:intron 25.0%
!! ATTGATGGATATTGTATTGA+GGG - chr3_1:17056429-17056448 None:intergenic 25.0%
!!! ACTTTTTCACTAAACATTGA+TGG - chr3_1:17056444-17056463 None:intergenic 25.0%
! TCTAATATAATCCAAGAGAA+TGG - chr3_1:17056473-17056492 None:intergenic 25.0%
! TATTAGACAATGTTTAGTGA+TGG + chr3_1:17056486-17056505 Msa0351110:intron 25.0%
! TTACCAAATGTATAATCACA+TGG + chr3_1:17056641-17056660 Msa0351110:intron 25.0%
! TTTCCATGTGATTATACATT+TGG - chr3_1:17056647-17056666 None:intergenic 25.0%
!! TTAGATTCAAAATGTTTCTG+TGG + chr3_1:17056816-17056835 Msa0351110:intron 25.0%
!! TAGATTCAAAATGTTTCTGT+GGG + chr3_1:17056817-17056836 Msa0351110:intron 25.0%
! ATTCCAATTGAATTCTTGAA+AGG + chr3_1:17056845-17056864 Msa0351110:intron 25.0%
! GGAGTAAAATGATGAAAATA+AGG - chr3_1:17057085-17057104 None:intergenic 25.0%
! AACTAATCAAATCAAGTGTA+GGG - chr3_1:17057106-17057125 None:intergenic 25.0%
! AAATCAGGATGATTACAAAT+TGG + chr3_1:17057218-17057237 Msa0351110:intron 25.0%
!!! ATTACAAATTGGTTGATGAA+AGG + chr3_1:17057229-17057248 Msa0351110:intron 25.0%
!!! GTTTTGTTCAATCATAAAGT+TGG - chr3_1:17057273-17057292 None:intergenic 25.0%
! TTCTTAGACTTAATACATCT+TGG + chr3_1:17057521-17057540 Msa0351110:intron 25.0%
!! GGATTTGTTTATTACTAAAG+GGG - chr3_1:17057570-17057589 None:intergenic 25.0%
! ATGTATACTGATAAATCTGT+TGG - chr3_1:17057641-17057660 None:intergenic 25.0%
! ACATCTCATAATTTCAAAAC+AGG - chr3_1:17057863-17057882 None:intergenic 25.0%
! GATAGATAAAAAAAGGGAAT+AGG - chr3_1:17057969-17057988 None:intergenic 25.0%
! AAGAAGTCAAAATCATATCA+GGG - chr3_1:17058014-17058033 None:intergenic 25.0%
!!! ATTTTGACTTCTTGTAATCA+AGG + chr3_1:17058021-17058040 Msa0351110:intron 25.0%
! TCTCAAAATAATAAGCATCA+AGG - chr3_1:17058116-17058135 None:intergenic 25.0%
! TTGTACTAGACATAATGTAT+AGG - chr3_1:17058288-17058307 None:intergenic 25.0%
AGATAGCTCAATTTCGAAAT+AGG - chr3_1:17054607-17054626 None:intergenic 30.0%
AGTTTGACCAGTAAAAGAAA+AGG - chr3_1:17054646-17054665 None:intergenic 30.0%
AAACTATGCTGAAGATCTAT+GGG + chr3_1:17054661-17054680 Msa0351110:exon 30.0%
!! TTTATAGGCATGTTTGGTTT+TGG + chr3_1:17054835-17054854 Msa0351110:intron 30.0%
! AACTTCAAGTTATACAAGGA+AGG - chr3_1:17055036-17055055 None:intergenic 30.0%
AAAAAGTAAGTTATCACCAG+AGG - chr3_1:17055173-17055192 None:intergenic 30.0%
! TTTTCACTCCCAATACTATT+AGG - chr3_1:17055407-17055426 None:intergenic 30.0%
ATGCTAAGTGATCGATAATT+AGG + chr3_1:17055563-17055582 Msa0351110:intron 30.0%
ACCAATAGTCATATTTGCTT+TGG + chr3_1:17055613-17055632 Msa0351110:intron 30.0%
TCCAAAGCAAATATGACTAT+TGG - chr3_1:17055617-17055636 None:intergenic 30.0%
AACAATGACTTGATTGGTAA+GGG - chr3_1:17055902-17055921 None:intergenic 30.0%
ATTTGTTTGATGCTTAAGTG+GGG - chr3_1:17055936-17055955 None:intergenic 30.0%
! GATTTGTTTGATGCTTAAGT+GGG - chr3_1:17055937-17055956 None:intergenic 30.0%
!! TGATTTGTTTGATGCTTAAG+TGG - chr3_1:17055938-17055957 None:intergenic 30.0%
! GATTTGATTTAATATGGGCT+GGG - chr3_1:17055963-17055982 None:intergenic 30.0%
! TGATTTGATTTAATATGGGC+TGG - chr3_1:17055964-17055983 None:intergenic 30.0%
! AACCCAATTCACATCATTTT+TGG + chr3_1:17055984-17056003 Msa0351110:intron 30.0%
!! ACCCAATTCACATCATTTTT+GGG + chr3_1:17055985-17056004 Msa0351110:intron 30.0%
TCCCAAAAATGATGTGAATT+GGG - chr3_1:17055989-17056008 None:intergenic 30.0%
ATCCCAAAAATGATGTGAAT+TGG - chr3_1:17055990-17056009 None:intergenic 30.0%
AATCTATAGTGACTATAGTG+GGG - chr3_1:17056028-17056047 None:intergenic 30.0%
CAATCTATAGTGACTATAGT+GGG - chr3_1:17056029-17056048 None:intergenic 30.0%
ATTAAAAAAAAAGGGCAACC+CGG + chr3_1:17056240-17056259 Msa0351110:intron 30.0%
! TTGATGGATATTGTATTGAG+GGG - chr3_1:17056428-17056447 None:intergenic 30.0%
! CATTGATGGATATTGTATTG+AGG - chr3_1:17056430-17056449 None:intergenic 30.0%
CAACCTTTCAAGAATTCAAT+TGG - chr3_1:17056851-17056870 None:intergenic 30.0%
TTCTTGAAAGGTTGTATCAT+TGG + chr3_1:17056857-17056876 Msa0351110:intron 30.0%
TAGCGTTCTTAAATCTAATG+TGG + chr3_1:17056893-17056912 Msa0351110:intron 30.0%
! ACATTCATGGGAGTATAAAA+AGG - chr3_1:17056999-17057018 None:intergenic 30.0%
AAGAATATCATGTATCCACA+TGG - chr3_1:17057023-17057042 None:intergenic 30.0%
CAACTAATCAAATCAAGTGT+AGG - chr3_1:17057107-17057126 None:intergenic 30.0%
AATTAATTAGGACCCCATTT+AGG + chr3_1:17057344-17057363 Msa0351110:intron 30.0%
ACGTTCACAATATACAAAAG+GGG - chr3_1:17057724-17057743 None:intergenic 30.0%
CACGTTCACAATATACAAAA+GGG - chr3_1:17057725-17057744 None:intergenic 30.0%
ACACGTTCACAATATACAAA+AGG - chr3_1:17057726-17057745 None:intergenic 30.0%
GCTAACATGAATCAATGAAT+TGG + chr3_1:17057747-17057766 Msa0351110:intron 30.0%
ATCAATGAATTGGCATACAT+TGG + chr3_1:17057757-17057776 Msa0351110:intron 30.0%
ACAATGAATGAAGAGACAAA+GGG - chr3_1:17057794-17057813 None:intergenic 30.0%
AACAATGAATGAAGAGACAA+AGG - chr3_1:17057795-17057814 None:intergenic 30.0%
AAAATCATATCAGGGACAAA+TGG - chr3_1:17058006-17058025 None:intergenic 30.0%
CAAGAAGTCAAAATCATATC+AGG - chr3_1:17058015-17058034 None:intergenic 30.0%
!!! TTTTGAGATGTGTTTACTTG+TGG + chr3_1:17058128-17058147 Msa0351110:three_prime_UTR 30.0%
! TTTGAGATGTGTTTACTTGT+GGG + chr3_1:17058129-17058148 Msa0351110:three_prime_UTR 30.0%
! AAAGAAAAGGTACGAAGACA+TGG - chr3_1:17054633-17054652 None:intergenic 35.0%
! CTTCGTACCTTTTCTTTTAC+TGG + chr3_1:17054636-17054655 Msa0351110:five_prime_UTR 35.0%
CAAACTATGCTGAAGATCTA+TGG + chr3_1:17054660-17054679 Msa0351110:exon 35.0%
TTACATGCTTGATAGAAGAG+AGG - chr3_1:17054698-17054717 None:intergenic 35.0%
! AAACATGCCTATAAAGTACC+TGG - chr3_1:17054830-17054849 None:intergenic 35.0%
AGGTACTTTATAGGCATGTT+TGG + chr3_1:17054829-17054848 Msa0351110:intron 35.0%
! CAGCAACTTCAAGTTATACA+AGG - chr3_1:17055040-17055059 None:intergenic 35.0%
AACTTGAAGTTGCTGAAAGT+AGG + chr3_1:17055045-17055064 Msa0351110:CDS 35.0%
AAAAAGTCTACTATTCGACC+AGG - chr3_1:17055093-17055112 None:intergenic 35.0%
GTTAACGTGATAACTTACTC+TGG + chr3_1:17055138-17055157 Msa0351110:CDS 35.0%
!!! ACTTACTTTTTAAGGTGAGG+TGG + chr3_1:17055182-17055201 Msa0351110:intron 35.0%
!! ACAAACTTGACTTTGAAGCA+AGG + chr3_1:17055342-17055361 Msa0351110:intron 35.0%
AAGCAAGGATAAAGCTAAAG+TGG + chr3_1:17055357-17055376 Msa0351110:intron 35.0%
GTGATCGATAATTAGGTACT+AGG + chr3_1:17055570-17055589 Msa0351110:intron 35.0%
!! ATTTGCTTTGGAGATTCAGA+TGG + chr3_1:17055625-17055644 Msa0351110:intron 35.0%
AGGTAACTGAAAATGGGATT+TGG + chr3_1:17055665-17055684 Msa0351110:intron 35.0%
TAAGGGATTTGAATCACATG+TGG - chr3_1:17055885-17055904 None:intergenic 35.0%
CAACAATGACTTGATTGGTA+AGG - chr3_1:17055903-17055922 None:intergenic 35.0%
CTTACCAATCAAGTCATTGT+TGG + chr3_1:17055901-17055920 Msa0351110:intron 35.0%
ACTACCAACAATGACTTGAT+TGG - chr3_1:17055908-17055927 None:intergenic 35.0%
!!! CATCATTTTTGGGATGTTAG+AGG + chr3_1:17055995-17056014 Msa0351110:intron 35.0%
GCAATCTATAGTGACTATAG+TGG - chr3_1:17056030-17056049 None:intergenic 35.0%
AGAAAAGACCAAAAAGAGTG+TGG + chr3_1:17056180-17056199 Msa0351110:intron 35.0%
! GAAAAGACCAAAAAGAGTGT+GGG + chr3_1:17056181-17056200 Msa0351110:intron 35.0%
TCTTGTGTAAAAACTGGGTA+AGG - chr3_1:17056332-17056351 None:intergenic 35.0%
! TTACCCAGTTTTTACACAAG+AGG + chr3_1:17056331-17056350 Msa0351110:intron 35.0%
AAAAGTTGTTGTCATGTGAC+TGG - chr3_1:17056386-17056405 None:intergenic 35.0%
AAAGTTATGAGCCATTCTCT+TGG + chr3_1:17056459-17056478 Msa0351110:intron 35.0%
!! TGGATGCTCTAAAATTCATG+AGG + chr3_1:17056968-17056987 Msa0351110:intron 35.0%
TAGTCCTAGAGATCCTAAAT+GGG - chr3_1:17057360-17057379 None:intergenic 35.0%
TTAGTCCTAGAGATCCTAAA+TGG - chr3_1:17057361-17057380 None:intergenic 35.0%
AACATTCCTGTAGCACATTT+GGG + chr3_1:17057688-17057707 Msa0351110:intron 35.0%
AGGAGAAAATGACGAAAACA+TGG + chr3_1:17058069-17058088 Msa0351110:three_prime_UTR 35.0%
!! CTTCGTTGATTCTACTTAGC+TGG + chr3_1:17054754-17054773 Msa0351110:CDS 40.0%
ATGCTCTCCAGGTACTTTAT+AGG + chr3_1:17054820-17054839 Msa0351110:intron 40.0%
CTGATCCTAATACTTCCTGA+TGG + chr3_1:17054966-17054985 Msa0351110:intron 40.0%
!! CTAAGCCATCAGGAAGTATT+AGG - chr3_1:17054974-17054993 None:intergenic 40.0%
CTAATACTTCCTGATGGCTT+AGG + chr3_1:17054972-17054991 Msa0351110:intron 40.0%
CTTCGATAATTCAGGAACTG+AGG - chr3_1:17055004-17055023 None:intergenic 40.0%
CTCAGTTCCTGAATTATCGA+AGG + chr3_1:17055002-17055021 Msa0351110:CDS 40.0%
AGCTCAACCTTCGATAATTC+AGG - chr3_1:17055012-17055031 None:intergenic 40.0%
TGAAGTTGCTGAAAGTAGGA+AGG + chr3_1:17055049-17055068 Msa0351110:CDS 40.0%
ATCACGTTAACCATTGCTGA+GGG - chr3_1:17055129-17055148 None:intergenic 40.0%
TATCACGTTAACCATTGCTG+AGG - chr3_1:17055130-17055149 None:intergenic 40.0%
GTCATGACTAGAGACATCAT+GGG - chr3_1:17055279-17055298 None:intergenic 40.0%
AGTCATGACTAGAGACATCA+TGG - chr3_1:17055280-17055299 None:intergenic 40.0%
CAAGGATAAAGCTAAAGTGG+TGG + chr3_1:17055360-17055379 Msa0351110:intron 40.0%
! GTTGCTATTGCTACGAGTAT+TGG + chr3_1:17055502-17055521 Msa0351110:intron 40.0%
! GTGTAGGAGGTATAATGTAG+TGG + chr3_1:17055527-17055546 Msa0351110:intron 40.0%
TGGCACATCAAGGAAGATAA+AGG + chr3_1:17055645-17055664 Msa0351110:intron 40.0%
TAGCATTCGGACATGTTTGT+TGG - chr3_1:17055723-17055742 None:intergenic 40.0%
ACATGTGGAGAGATTTGACT+TGG - chr3_1:17055870-17055889 None:intergenic 40.0%
CGTACAATACACCAAATGGT+GGG - chr3_1:17056306-17056325 None:intergenic 40.0%
GTTGCGTACAATACACCAAA+TGG - chr3_1:17056310-17056329 None:intergenic 40.0%
CAGCCTCTTGTGTAAAAACT+GGG - chr3_1:17056337-17056356 None:intergenic 40.0%
ACAGCCTCTTGTGTAAAAAC+TGG - chr3_1:17056338-17056357 None:intergenic 40.0%
ATTGTATTGAGGGGTAACCT+TGG - chr3_1:17056419-17056438 None:intergenic 40.0%
!! TGAGAGCATTAACTCATGCT+AGG - chr3_1:17056533-17056552 None:intergenic 40.0%
TAATGCTCTCATTAGCGTAC+AGG + chr3_1:17056542-17056561 Msa0351110:intron 40.0%
CTAGGTACTTGTGTGATAGT+CGG + chr3_1:17056573-17056592 Msa0351110:intron 40.0%
!! GCTCTAAAATTCATGAGGTC+AGG + chr3_1:17056973-17056992 Msa0351110:intron 40.0%
TATCCACATGGCACATTCAT+GGG - chr3_1:17057011-17057030 None:intergenic 40.0%
AGTCCTAGAGATCCTAAATG+GGG - chr3_1:17057359-17057378 None:intergenic 40.0%
!!! AGGAACTTTTGAGGCATTGA+TGG - chr3_1:17057591-17057610 None:intergenic 40.0%
ATCAATGCCTCAAAAGTTCC+TGG + chr3_1:17057590-17057609 Msa0351110:intron 40.0%
CATTGCAGTTGAAGAGGAAT+TGG - chr3_1:17057667-17057686 None:intergenic 40.0%
ATGTTGCATTGCAGTTGAAG+AGG - chr3_1:17057673-17057692 None:intergenic 40.0%
CAACATTCCTGTAGCACATT+TGG + chr3_1:17057687-17057706 Msa0351110:intron 40.0%
GATATTCCTCATCTTTGTGC+TGG + chr3_1:17057822-17057841 Msa0351110:intron 40.0%
ATGACAAAGCAGAAGAGCTA+AGG + chr3_1:17058049-17058068 Msa0351110:three_prime_UTR 40.0%
CATGGATGAAGCGAATGATA+AGG + chr3_1:17058087-17058106 Msa0351110:three_prime_UTR 40.0%
AAAGGGCTCGACACTATAGA+AGG - chr3_1:17054463-17054482 None:intergenic 45.0%
!! CTATAGTGTCGAGCCCTTTT+CGG + chr3_1:17054464-17054483 Msa0351110:five_prime_UTR 45.0%
GCTTGGGACTTGATATGCAA+AGG - chr3_1:17054551-17054570 None:intergenic 45.0%
AAGACATGTGCGTGATTCAG+CGG + chr3_1:17054728-17054747 Msa0351110:CDS 45.0%
! GTTGATTCTACTTAGCTGGC+TGG + chr3_1:17054758-17054777 Msa0351110:CDS 45.0%
!! CTGGAGAGCATCTAAAGAGA+CGG - chr3_1:17054811-17054830 None:intergenic 45.0%
AGGAAAGAACCTAAGCCATC+AGG - chr3_1:17054984-17055003 None:intergenic 45.0%
GTCTACTATTCGACCAGGTA+TGG - chr3_1:17055088-17055107 None:intergenic 45.0%
TCAAAACTCACCCTCAGCAA+TGG + chr3_1:17055116-17055135 Msa0351110:CDS 45.0%
ACTCTGGTCATAACTTCCTC+TGG + chr3_1:17055154-17055173 Msa0351110:CDS 45.0%
GAGATTCAGATGGCACATCA+AGG + chr3_1:17055635-17055654 Msa0351110:intron 45.0%
! AAGACCAAAAAGAGTGTGGG+TGG + chr3_1:17056184-17056203 Msa0351110:intron 45.0%
! AGACCAAAAAGAGTGTGGGT+GGG + chr3_1:17056185-17056204 Msa0351110:intron 45.0%
GCGTACAATACACCAAATGG+TGG - chr3_1:17056307-17056326 None:intergenic 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + chr3_1:17056342-17056361 Msa0351110:intron 45.0%
GTTGTTGTCATGTGACTGGA+AGG - chr3_1:17056382-17056401 None:intergenic 45.0%
! AACTTTTACCAGTTGCGCCA+AGG + chr3_1:17056399-17056418 Msa0351110:intron 45.0%
TTGCTTATCGACCTCATACG+AGG - chr3_1:17056601-17056620 None:intergenic 45.0%
GTATCCACATGGCACATTCA+TGG - chr3_1:17057012-17057031 None:intergenic 45.0%
TCCACCACACGTATCTCTAA+CGG + chr3_1:17057614-17057633 Msa0351110:intron 45.0%
AGTTGACCCAAATGTGCTAC+AGG - chr3_1:17057697-17057716 None:intergenic 45.0%
TGTAGCACATTTGGGTCAAC+TGG + chr3_1:17057696-17057715 Msa0351110:intron 45.0%
GTAGCACATTTGGGTCAACT+GGG + chr3_1:17057697-17057716 Msa0351110:intron 45.0%
ATTCCTCATCTTTGTGCTGG+TGG + chr3_1:17057825-17057844 Msa0351110:intron 45.0%
CGTCTCTTTAGATGCTCTCC+AGG + chr3_1:17054809-17054828 Msa0351110:CDS 50.0%
!! GCTACGAGTATTGGCTGTGT+AGG + chr3_1:17055511-17055530 Msa0351110:intron 50.0%
!! ACGAGTATTGGCTGTGTAGG+AGG + chr3_1:17055514-17055533 Msa0351110:intron 50.0%
TGGGATTTGGCTGCTGTAGT+TGG + chr3_1:17055678-17055697 Msa0351110:intron 50.0%
TAGATTGCCTTACCGACCAC+AGG + chr3_1:17056042-17056061 Msa0351110:intron 50.0%
AGATTGCCTTACCGACCACA+GGG + chr3_1:17056043-17056062 Msa0351110:intron 50.0%
ATAAAGACTGCCACACCCTG+TGG - chr3_1:17056061-17056080 None:intergenic 50.0%
! CATCCCACCCACACTCTTTT+TGG - chr3_1:17056191-17056210 None:intergenic 50.0%
GTGGGTGGGATGACTTCAAT+AGG + chr3_1:17056199-17056218 Msa0351110:intron 50.0%
ATGCGGGAACTTTAGTGCAC+CGG - chr3_1:17056262-17056281 None:intergenic 50.0%
CTAAAGTTCCCGCATACGCA+AGG + chr3_1:17056267-17056286 Msa0351110:intron 50.0%
TCATGTGACTGGAAGGTCAC+GGG - chr3_1:17056375-17056394 None:intergenic 50.0%
GTCATGTGACTGGAAGGTCA+CGG - chr3_1:17056376-17056395 None:intergenic 50.0%
TGCTCTCATTAGCGTACAGG+TGG + chr3_1:17056545-17056564 Msa0351110:intron 50.0%
AGCGTACAGGTGGTACTTCT+AGG + chr3_1:17056555-17056574 Msa0351110:intron 50.0%
ACTCCCATGAATGTGCCATG+TGG + chr3_1:17057005-17057024 Msa0351110:intron 50.0%
! GGACCCCATTTAGGATCTCT+AGG + chr3_1:17057353-17057372 Msa0351110:intron 50.0%
ACCGTTAGAGATACGTGTGG+TGG - chr3_1:17057618-17057637 None:intergenic 50.0%
TGGACCGTTAGAGATACGTG+TGG - chr3_1:17057621-17057640 None:intergenic 50.0%
ATGCCACCAGCACAAAGATG+AGG - chr3_1:17057831-17057850 None:intergenic 50.0%
TTCCCACGCTCATCCATACC+TGG + chr3_1:17055072-17055091 Msa0351110:CDS 55.0%
GACCAGGTATGGATGAGCGT+GGG - chr3_1:17055077-17055096 None:intergenic 55.0%
TGCGGGAACTTTAGTGCACC+GGG - chr3_1:17056261-17056280 None:intergenic 55.0%
AAGGTCACGGGTTCAAGTCC+TGG - chr3_1:17056363-17056382 None:intergenic 55.0%
! GATAGTCGGTGCCTCGTATG+AGG + chr3_1:17056587-17056606 Msa0351110:intron 55.0%
! GGTGGAGCCAGGAACTTTTG+AGG - chr3_1:17057600-17057619 None:intergenic 55.0%
CGACCAGGTATGGATGAGCG+TGG - chr3_1:17055078-17055097 None:intergenic 60.0%
AGACTGCCACACCCTGTGGT+CGG - chr3_1:17056057-17056076 None:intergenic 60.0%
GTTCCCGCATACGCAAGGTC+CGG + chr3_1:17056272-17056291 Msa0351110:intron 60.0%
TTCCCGCATACGCAAGGTCC+GGG + chr3_1:17056273-17056292 Msa0351110:intron 60.0%
TTCCCGGACCTTGCGTATGC+GGG - chr3_1:17056278-17056297 None:intergenic 60.0%
CTTCCCGGACCTTGCGTATG+CGG - chr3_1:17056279-17056298 None:intergenic 60.0%
GCATACGCAAGGTCCGGGAA+GGG + chr3_1:17056278-17056297 Msa0351110:intron 60.0%
CATACGCAAGGTCCGGGAAG+GGG + chr3_1:17056279-17056298 Msa0351110:intron 60.0%
! CAAATGGTGGGACCCCTTCC+CGG - chr3_1:17056294-17056313 None:intergenic 60.0%
AGGGGTAACCTTGGCGCAAC+TGG - chr3_1:17056410-17056429 None:intergenic 60.0%
! GAGATACGTGTGGTGGAGCC+AGG - chr3_1:17057611-17057630 None:intergenic 60.0%
GCCTTACCGACCACAGGGTG+TGG + chr3_1:17056048-17056067 Msa0351110:intron 65.0%
GCCACACCCTGTGGTCGGTA+AGG - chr3_1:17056052-17056071 None:intergenic 65.0%
CGCATACGCAAGGTCCGGGA+AGG + chr3_1:17056277-17056296 Msa0351110:intron 65.0%
CGGGAAGGGGTCCCACCATT+TGG + chr3_1:17056292-17056311 Msa0351110:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 17054350 17058318 17054350 ID=Msa0351110;Name=Msa0351110
chr3_1 mRNA 17054350 17058318 17054350 ID=Msa0351110-mRNA-1;Parent=Msa0351110;Name=Msa0351110-mRNA-1;_AED=0.03;_eAED=0.03;_QI=316|1|1|1|0.5|0.33|3|292|116
chr3_1 exon 17054350 17054830 17054350 ID=Msa0351110-mRNA-1:exon:4085;Parent=Msa0351110-mRNA-1
chr3_1 exon 17054994 17055195 17054994 ID=Msa0351110-mRNA-1:exon:4086;Parent=Msa0351110-mRNA-1
chr3_1 exon 17058043 17058318 17058043 ID=Msa0351110-mRNA-1:exon:4087;Parent=Msa0351110-mRNA-1
chr3_1 five_prime_UTR 17054350 17054665 17054350 ID=Msa0351110-mRNA-1:five_prime_utr;Parent=Msa0351110-mRNA-1
chr3_1 CDS 17054666 17054830 17054666 ID=Msa0351110-mRNA-1:cds;Parent=Msa0351110-mRNA-1
chr3_1 CDS 17054994 17055179 17054994 ID=Msa0351110-mRNA-1:cds;Parent=Msa0351110-mRNA-1
chr3_1 three_prime_UTR 17055180 17055195 17055180 ID=Msa0351110-mRNA-1:three_prime_utr;Parent=Msa0351110-mRNA-1
chr3_1 three_prime_UTR 17058043 17058318 17058043 ID=Msa0351110-mRNA-1:three_prime_utr;Parent=Msa0351110-mRNA-1
Gene Sequence

>Msa0351110

ATGCTGAAGATCTATGGGAGCAAGTGCAACCTCTCTTCTATCAAGCATGTAAATATTCATTTAAGACATGTGCGTGATTCAGCGGAGACTTCGTTGATTCTACTTAGCTGGCTGGTTGAGTTTGCTAGTATCGAATCATTGACCGTCTCTTTAGATGCTCTCCAGGTTCTTTCCTCAGTTCCTGAATTATCGAAGGTTGAGCTTCCTTCCTTGTATAACTTGAAGTTGCTGAAAGTAGGAAGGTTCCCACGCTCATCCATACCTGGTCGAATAGTAGACTTTTTGCTTCAAAACTCACCCTCAGCAATGGTTAACGTGATAACTTACTCTGGTCATAACTTCCTCTGGTGA
Protein sequence

>Msa0351110

MLKIYGSKCNLSSIKHVNIHLRHVRDSAETSLILLSWLVEFASIESLTVSLDALQVLSSVPELSKVELPSLYNLKLLKVGRFPRSSIPGRIVDFLLQNSPSAMVNVITYSGHNFLW*