AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0509420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0509420 MtrunA17_Chr3g0114351 99.087 438 4 0 1 438 1 438 0.0 860
Msa0509420 MtrunA17_Chr5g0437961 73.882 425 111 0 8 432 3 427 0.0 650
Msa0509420 MtrunA17_Chr5g0437951 54.920 437 184 4 12 436 11 446 4.07e-173 492
Msa0509420 MtrunA17_Chr7g0220531 47.196 428 220 1 10 431 29 456 6.41e-137 401
Msa0509420 MtrunA17_Chr8g0366021 46.763 417 219 1 25 438 48 464 1.91e-129 382
Msa0509420 MtrunA17_Chr6g0455911 44.165 437 234 4 10 438 20 454 1.52e-120 359
Msa0509420 MtrunA17_Chr6g0455951 42.237 438 243 4 9 438 24 459 6.56e-119 355
Msa0509420 MtrunA17_Chr6g0455981 42.290 428 238 3 10 429 22 448 7.34e-119 354
Msa0509420 MtrunA17_Chr6g0455941 43.510 416 232 2 25 438 48 462 9.32e-115 344
Msa0509420 MtrunA17_Chr2g0333321 39.815 432 251 5 10 438 62 487 1.45e-107 327
Msa0509420 MtrunA17_Chr8g0366001 45.896 268 142 1 174 438 4 271 5.54e-78 243
Msa0509420 MtrunA17_Chr6g0459871 40.824 267 155 2 174 438 50 315 6.61e-61 200
Msa0509420 MtrunA17_Chr3g0114341 77.982 109 24 0 4 112 2 110 7.16e-49 162
Msa0509420 MtrunA17_Chr8g0365971 46.296 162 86 1 25 186 52 212 1.88e-39 141
Msa0509420 MtrunA17_Chr8g0365991 50.467 107 53 0 25 131 48 154 4.68e-25 101
Msa0509420 MtrunA17_Chr4g0022261 21.759 432 297 13 14 436 124 523 4.23e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0509420 AT3G56200.1 59.314 408 166 0 25 432 24 431 5.19e-174 494
Msa0509420 AT2G40420.1 58.469 431 174 2 6 431 2 432 4.66e-171 487
Msa0509420 AT3G30390.1 45.872 436 230 1 2 431 15 450 2.85e-132 389
Msa0509420 AT3G30390.3 45.872 436 230 1 2 431 15 450 2.85e-132 389
Msa0509420 AT3G30390.2 45.872 436 230 1 2 431 15 450 2.85e-132 389
Msa0509420 AT5G38820.1 44.860 428 228 2 12 431 17 444 8.26e-129 380
Msa0509420 AT5G38820.3 44.860 428 228 2 12 431 17 444 8.26e-129 380
Msa0509420 AT5G38820.2 44.860 428 228 2 12 431 22 449 1.37e-128 380
Msa0509420 AT1G80510.1 40.460 435 245 6 10 438 62 488 1.79e-97 301
Msa0509420 AT2G39130.2 23.611 432 271 13 19 435 155 542 9.10e-11 64.3
Msa0509420 AT2G39130.1 23.611 432 271 13 19 435 155 542 9.10e-11 64.3

Find 124 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTTACAATCGGTCTATT+TGG 0.163160 3_4:+57874422 Msa0509420:CDS
GCATTTGTTGCCATCTGTTC+TGG 0.170280 3_4:+57874066 Msa0509420:CDS
ATATACAAAATTCATATAAA+TGG 0.184445 3_4:+57872798 None:intergenic
TGAAAGAAGTACCAAATATC+TGG 0.234058 3_4:-57874719 None:intergenic
GGTTCTAACCAATGATAATT+AGG 0.245044 3_4:-57873250 None:intergenic
GTGGCTAGCAGAGGCTTCTT+AGG 0.255194 3_4:-57874626 None:intergenic
TGGTACTTCTTTCAGTTTCT+AGG 0.268753 3_4:+57874728 Msa0509420:CDS
TCTAATCGAGGAACAAGTTT+AGG 0.285115 3_4:-57874129 None:intergenic
TCAAATCCGGCGGCGGTTCC+TGG 0.299360 3_4:+57872945 Msa0509420:CDS
CTATAGCAATCCCAGATATT+TGG 0.301975 3_4:+57874708 Msa0509420:CDS
GTGTTATAATCACAAACTTT+GGG 0.314828 3_4:+57873213 Msa0509420:CDS
TCATTATCTTGTCAAATAAA+AGG 0.328980 3_4:+57875392 Msa0509420:three_prime_UTR
GGGGAGTTGGGGCGTGGAAG+GGG 0.330652 3_4:+57875121 Msa0509420:three_prime_UTR
CTAATCGAGGAACAAGTTTA+GGG 0.336457 3_4:-57874128 None:intergenic
TTACTTGCAGTACATCCTAT+TGG 0.338534 3_4:+57874329 Msa0509420:intron
TACTTGCAGTACATCCTATT+GGG 0.340851 3_4:+57874330 Msa0509420:intron
TGGGTTGGCTATTGTTGCTT+TGG 0.345042 3_4:+57874086 Msa0509420:CDS
CATTTGTTGCCATCTGTTCT+GGG 0.345258 3_4:+57874067 Msa0509420:CDS
CTCAAAGAGAAATTTATAAG+AGG 0.349290 3_4:-57874578 None:intergenic
GGTTCTTGTTATGCTTCCTT+TGG 0.349741 3_4:+57873656 Msa0509420:CDS
GTGTTGAATCTCCAAATAAA+AGG 0.361617 3_4:-57874448 None:intergenic
CAGAAACGTGTGACAAAGTT+GGG 0.369400 3_4:+57875280 Msa0509420:three_prime_UTR
GGTGTTATGAAGGAAGCTTT+TGG 0.378184 3_4:+57873161 Msa0509420:CDS
GCATTCACATTTCATTTCAA+TGG 0.380536 3_4:+57874204 Msa0509420:CDS
GTTCTCTTCCTAATTATCAT+TGG 0.381754 3_4:+57873242 Msa0509420:CDS
CTCCGGAATCATGTCAATTC+CGG 0.385246 3_4:+57873013 Msa0509420:CDS
TGTGTTATAATCACAAACTT+TGG 0.385504 3_4:+57873212 Msa0509420:CDS
AATGTCGCCACCAGCATAAT+CGG 0.388972 3_4:+57872990 Msa0509420:CDS
GAATCCTTGAAGTACAGTTC+TGG 0.395671 3_4:+57874021 Msa0509420:intron
CGCCGGAATTGACATGATTC+CGG 0.403675 3_4:-57873015 None:intergenic
AAGAAAATCAAAGAAACATA+AGG 0.406357 3_4:-57875176 None:intergenic
TATTCCAGAACTGTACTTCA+AGG 0.410500 3_4:-57874025 None:intergenic
GCAGAGGCTTCTTAGGGAAT+AGG 0.417972 3_4:-57874619 None:intergenic
AACAGTGGTTTGGGATTCAT+TGG 0.424191 3_4:+57873598 Msa0509420:CDS
GGATTCATTGGTGGAATTCA+AGG 0.427676 3_4:+57873610 Msa0509420:CDS
GGGAGTTGAGAGAGGAAATG+TGG 0.430334 3_4:-57872854 None:intergenic
GATTGCTATAGCAGCAAGAT+AGG 0.434216 3_4:-57874696 None:intergenic
CCGGCGATTCTCAAAGTTCT+CGG 0.438035 3_4:+57873032 Msa0509420:CDS
AGAAAGCAACAACAAAAGAA+TGG 0.451699 3_4:-57875078 None:intergenic
GCGGTTCCTGGTTCAATCCC+TGG 0.452034 3_4:+57872957 Msa0509420:CDS
GCGACATTGAATACGGCGCC+AGG 0.467060 3_4:-57872975 None:intergenic
GCTGTTGTCTACTTTACAAT+CGG 0.469071 3_4:+57874413 Msa0509420:CDS
CTTGCAAGCTCAAACCCAAT+AGG 0.469781 3_4:-57874344 None:intergenic
CAGAATGCTGATTCTGCTGT+TGG 0.471631 3_4:+57874497 Msa0509420:CDS
TGTTGCCATCTGTTCTGGGT+TGG 0.478955 3_4:+57874071 Msa0509420:CDS
ACAAATCAAAGAATGATGTT+TGG 0.480585 3_4:-57874155 None:intergenic
AACCGCCGCCGGATTTGATC+CGG 0.483830 3_4:-57872940 None:intergenic
TACCTTAAAACAATAGTGCC+AGG 0.484684 3_4:-57874785 None:intergenic
GAAAGCAACAACAAAAGAAT+GGG 0.486157 3_4:-57875077 None:intergenic
TAAAAGGTCCCATGTGATGA+TGG 0.491633 3_4:+57875408 Msa0509420:three_prime_UTR
TGGCTAGCAGAGGCTTCTTA+GGG 0.492601 3_4:-57874625 None:intergenic
GTGAATGCTGTGACTACAAC+AGG 0.493679 3_4:-57874189 None:intergenic
AGAAAATCAAAGAAACATAA+GGG 0.496814 3_4:-57875175 None:intergenic
GGTGTAACCACGACGTACTC+TGG 0.498670 3_4:+57873140 Msa0509420:CDS
ATCAACAACAGCACTATGCC+TGG 0.499325 3_4:+57874751 Msa0509420:CDS
AAGTGGAGGTGAAGTGCACT+TGG 0.500293 3_4:+57873566 Msa0509420:CDS
ATAAGAATCAAGGCGAAAGA+AGG 0.506142 3_4:-57873062 None:intergenic
GCAGAAACGTGTGACAAAGT+TGG 0.511040 3_4:+57875279 Msa0509420:three_prime_UTR
TAAAGCATTGTATATGTTGG+TGG 0.512729 3_4:-57875022 None:intergenic
TCTCTTGACCGGATCAAATC+CGG 0.513994 3_4:+57872932 Msa0509420:CDS
GCTGGTGGCGACATTGAATA+CGG 0.515321 3_4:-57872982 None:intergenic
TCTAATGACAGGGATGCTCT+TGG 0.515706 3_4:+57874922 Msa0509420:intron
GCTATTGTTGCTTTGGTGCA+AGG 0.520668 3_4:+57874093 Msa0509420:CDS
GTCAACGTCCCTCTCTTGAC+CGG 0.521264 3_4:+57872921 Msa0509420:CDS
GAGAAAATCAACAGAAACTT+CGG 0.523044 3_4:-57873099 None:intergenic
TAAGTAGCAAGTCATAATGT+TGG 0.523113 3_4:+57875043 Msa0509420:exon
CAAAACCAACAAAATGAGCC+TGG 0.523599 3_4:-57875152 None:intergenic
GAAAGAAGTACCAAATATCT+GGG 0.527104 3_4:-57874718 None:intergenic
GACCGGATCAAATCCGGCGG+CGG 0.531549 3_4:+57872938 Msa0509420:CDS
AGTGGTTTGGGATTCATTGG+TGG 0.535144 3_4:+57873601 Msa0509420:CDS
AACAGAAACTTCGGCGAGCA+CGG 0.535273 3_4:-57873090 None:intergenic
GAATAGGAGTTCATCAATGT+TGG 0.539647 3_4:-57874603 None:intergenic
TCAAAGAGAAATTTATAAGA+GGG 0.545469 3_4:-57874577 None:intergenic
GACGTACTCTGGTGTTATGA+AGG 0.546221 3_4:+57873151 Msa0509420:CDS
TTGTTGTCTGTGGCTAGCAG+AGG 0.547461 3_4:-57874635 None:intergenic
CAAGAGAGGGACGTTGACTC+CGG 0.550502 3_4:-57872916 None:intergenic
GCCACCAGCATAATCGGCTC+CGG 0.553474 3_4:+57872996 Msa0509420:CDS
TGCCAGGGAAAATAAAAGCC+AGG 0.554102 3_4:-57874769 None:intergenic
ATTGAACCAGGAACCGCCGC+CGG 0.560640 3_4:-57872951 None:intergenic
TGTTATAATCACAAACTTTG+GGG 0.562562 3_4:+57873214 Msa0509420:CDS
TTCTTGTGGATTGCACGACA+AGG 0.566718 3_4:-57875441 None:intergenic
ACAGGACAAAATTATAGCAC+TGG 0.566982 3_4:+57874957 Msa0509420:CDS
GATTCCGGAGCCGATTATGC+TGG 0.567198 3_4:-57873000 None:intergenic
ATGTCACCAACACCGTCTGC+CGG 0.570180 3_4:+57872897 Msa0509420:CDS
AATAGCCAACCCAGAACAGA+TGG 0.570854 3_4:-57874076 None:intergenic
CACAAATCTCTTGTTGTCTG+TGG 0.572225 3_4:-57874645 None:intergenic
CACGGCGACGATAAGAATCA+AGG 0.576008 3_4:-57873072 None:intergenic
GATTCATTGGTGGAATTCAA+GGG 0.579413 3_4:+57873611 Msa0509420:CDS
CTATGCACTCCACATTATGT+TGG 0.580938 3_4:+57874550 Msa0509420:CDS
CCGAGAACTTTGAGAATCGC+CGG 0.581794 3_4:-57873032 None:intergenic
AGACGGTGTTGGTGACATCC+CGG 0.584616 3_4:-57872892 None:intergenic
CACTCAAACAATAACTAGCT+AGG 0.585576 3_4:-57875356 None:intergenic
AATAACTAGCTAGGTTCACA+TGG 0.589905 3_4:-57875347 None:intergenic
TTGACTCCGGCAGACGGTGT+TGG 0.599042 3_4:-57872903 None:intergenic
ACTTAAAGCATTGTATATGT+TGG 0.600971 3_4:-57875025 None:intergenic
GAGGGAATACCAACATAATG+TGG 0.601359 3_4:-57874559 None:intergenic
TCTTGTGGATTGCACGACAA+GGG 0.602539 3_4:-57875440 None:intergenic
ATTTGACAAGATAATGACAT+GGG 0.602584 3_4:-57875386 None:intergenic
TCTTGGCATATCAACAAGAC+AGG 0.602838 3_4:+57874939 Msa0509420:CDS
ACGTATTAAACTATTGAACA+AGG 0.608704 3_4:-57874522 None:intergenic
GATTTGATCCGGTCAAGAGA+GGG 0.609106 3_4:-57872929 None:intergenic
TTGGTCTTGTACAAACGTGT+AGG 0.611958 3_4:+57873675 Msa0509420:CDS
ACCTTAAAACAATAGTGCCA+GGG 0.615343 3_4:-57874784 None:intergenic
TCCGGAGCCGATTATGCTGG+TGG 0.619780 3_4:-57872997 None:intergenic
GGATTTGATCCGGTCAAGAG+AGG 0.624870 3_4:-57872930 None:intergenic
AATATCAACTCTTCTTGCTG+TGG 0.627616 3_4:+57874044 Msa0509420:CDS
GGGACGTTGACTCCGGCAGA+CGG 0.628323 3_4:-57872909 None:intergenic
ACGGCGCCAGGGATTGAACC+AGG 0.629078 3_4:-57872963 None:intergenic
CGACATTGAATACGGCGCCA+GGG 0.630121 3_4:-57872974 None:intergenic
TTGAGCGCTTAAAGCTCCAA+CGG 0.631364 3_4:-57873186 None:intergenic
TTATCTGGAAAGGAAAGTGG+AGG 0.631552 3_4:+57873552 Msa0509420:CDS
AGAGTACGTCGTGGTTACAC+CGG 0.641853 3_4:-57873138 None:intergenic
TAATCGAGGAACAAGTTTAG+GGG 0.642883 3_4:-57874127 None:intergenic
GTTATAATCACAAACTTTGG+GGG 0.644288 3_4:+57873215 Msa0509420:CDS
TATTTGACAAGATAATGACA+TGG 0.644984 3_4:-57875387 None:intergenic
AGTGGAGGTGAAGTGCACTT+GGG 0.652737 3_4:+57873567 Msa0509420:CDS
TTTCATTACCATCATCACAT+GGG 0.659902 3_4:-57875416 None:intergenic
ACACGTTTGTACAAGACCAA+AGG 0.661754 3_4:-57873672 None:intergenic
GATGTTTGGTAGTCTAATCG+AGG 0.668855 3_4:-57874141 None:intergenic
AGGAAGCATAACAAGAACCA+AGG 0.686365 3_4:-57873652 None:intergenic
AGAAACGTGTGACAAAGTTG+GGG 0.694303 3_4:+57875281 Msa0509420:three_prime_UTR
CTTGACCGGATCAAATCCGG+CGG 0.711799 3_4:+57872935 Msa0509420:CDS
CATAACACCAGAGTACGTCG+TGG 0.777944 3_4:-57873147 None:intergenic
CGGTGTTGGTGACATCCCGG+CGG 0.797506 3_4:-57872889 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTAATTATTATTCTATTAA+AGG + chr3_4:57874827-57874846 Msa0509420:intron 10.0%
!! ATAAAAGAAACAGATTAAAT+TGG - chr3_4:57873274-57873293 None:intergenic 15.0%
!! AAACAAAAAATTAATCCAAA+AGG - chr3_4:57873343-57873362 None:intergenic 15.0%
!! TATTTATGAAAGTTTGAATT+TGG + chr3_4:57873369-57873388 Msa0509420:intron 15.0%
!! ATTTATGAAAGTTTGAATTT+GGG + chr3_4:57873370-57873389 Msa0509420:intron 15.0%
!!! ATTATTTGTTCCTTTTTTAA+GGG + chr3_4:57874243-57874262 Msa0509420:intron 15.0%
!! ATTAGAAACAAAGAAAATTT+AGG - chr3_4:57874908-57874927 None:intergenic 15.0%
!!! AAGTTTTACACTTTAAAGTT+TGG + chr3_4:57873427-57873446 Msa0509420:intron 20.0%
!!! CATTATTTGTTCCTTTTTTA+AGG + chr3_4:57874242-57874261 Msa0509420:intron 20.0%
!! TCAAAGAGAAATTTATAAGA+GGG - chr3_4:57874580-57874599 None:intergenic 20.0%
!!! TCTACTACAAAAAGTTTTAT+TGG + chr3_4:57875098-57875117 Msa0509420:three_prime_UTR 20.0%
!!! CTACTACAAAAAGTTTTATT+GGG + chr3_4:57875099-57875118 Msa0509420:three_prime_UTR 20.0%
!!! TACTACAAAAAGTTTTATTG+GGG + chr3_4:57875100-57875119 Msa0509420:three_prime_UTR 20.0%
!! AGAAAATCAAAGAAACATAA+GGG - chr3_4:57875178-57875197 None:intergenic 20.0%
!! AAGAAAATCAAAGAAACATA+AGG - chr3_4:57875179-57875198 None:intergenic 20.0%
!! TCATTATCTTGTCAAATAAA+AGG + chr3_4:57875392-57875411 Msa0509420:three_prime_UTR 20.0%
! TGTGTTATAATCACAAACTT+TGG + chr3_4:57873212-57873231 Msa0509420:CDS 25.0%
! GTGTTATAATCACAAACTTT+GGG + chr3_4:57873213-57873232 Msa0509420:CDS 25.0%
! TGTTATAATCACAAACTTTG+GGG + chr3_4:57873214-57873233 Msa0509420:CDS 25.0%
! AGCTCTCTTAATTAATGATT+TGG - chr3_4:57873310-57873329 None:intergenic 25.0%
!! AAAGATAGGTTTTTGAATGA+TGG + chr3_4:57873472-57873491 Msa0509420:intron 25.0%
!! AAGATAGGTTTTTGAATGAT+GGG + chr3_4:57873473-57873492 Msa0509420:intron 25.0%
! CACTAAGAATTAAGAAATCA+TGG - chr3_4:57873724-57873743 None:intergenic 25.0%
! TTGAAGTTTCATCTAAATCA+TGG + chr3_4:57873864-57873883 Msa0509420:intron 25.0%
! TGAATATGACAAAATCACAA+TGG - chr3_4:57873892-57873911 None:intergenic 25.0%
!!! ACTTTTTTCGTTTTGAATTG+AGG + chr3_4:57873930-57873949 Msa0509420:intron 25.0%
! ACAAATCAAAGAATGATGTT+TGG - chr3_4:57874158-57874177 None:intergenic 25.0%
! ACGTATTAAACTATTGAACA+AGG - chr3_4:57874525-57874544 None:intergenic 25.0%
! CTCAAAGAGAAATTTATAAG+AGG - chr3_4:57874581-57874600 None:intergenic 25.0%
! TTCTATTAAAGGTGACAAAT+TGG + chr3_4:57874838-57874857 Msa0509420:intron 25.0%
! ACAAATTGGAAACAGAATAA+AGG + chr3_4:57874852-57874871 Msa0509420:intron 25.0%
!!! TTTTCTTTGTTTCTAATGAC+AGG + chr3_4:57874911-57874930 Msa0509420:intron 25.0%
!!! TTTCTTTGTTTCTAATGACA+GGG + chr3_4:57874912-57874931 Msa0509420:intron 25.0%
!!! ACTTAAAGCATTGTATATGT+TGG - chr3_4:57875028-57875047 None:intergenic 25.0%
!!! ACTACAAAAAGTTTTATTGG+GGG + chr3_4:57875101-57875120 Msa0509420:three_prime_UTR 25.0%
! ATTTGACAAGATAATGACAT+GGG - chr3_4:57875389-57875408 None:intergenic 25.0%
! TATTTGACAAGATAATGACA+TGG - chr3_4:57875390-57875409 None:intergenic 25.0%
GAGAAAATCAACAGAAACTT+CGG - chr3_4:57873102-57873121 None:intergenic 30.0%
GTTATAATCACAAACTTTGG+GGG + chr3_4:57873215-57873234 Msa0509420:CDS 30.0%
GTTCTCTTCCTAATTATCAT+TGG + chr3_4:57873242-57873261 Msa0509420:CDS 30.0%
! GGTTCTAACCAATGATAATT+AGG - chr3_4:57873253-57873272 None:intergenic 30.0%
GTTTGTGATGTTGAAAAGAT+AGG + chr3_4:57873458-57873477 Msa0509420:intron 30.0%
! TTTTTGATGTTTGTGTGTGA+TGG + chr3_4:57873513-57873532 Msa0509420:intron 30.0%
CTTAATTCTTAGTGCTTGTT+TGG + chr3_4:57873730-57873749 Msa0509420:intron 30.0%
!!! CTTCGTATATTTTGGTATCA+CGG - chr3_4:57873789-57873808 None:intergenic 30.0%
! AACATGGACTTCGTATATTT+TGG - chr3_4:57873797-57873816 None:intergenic 30.0%
AAATATACGAAGTCCATGTT+CGG + chr3_4:57873797-57873816 Msa0509420:intron 30.0%
! AAACGAAAAAAGTGCAAGTT+CGG - chr3_4:57873923-57873942 None:intergenic 30.0%
TTTGAATTGAGGAGATTAAG+CGG + chr3_4:57873941-57873960 Msa0509420:intron 30.0%
GCATTCACATTTCATTTCAA+TGG + chr3_4:57874204-57874223 Msa0509420:CDS 30.0%
TACTTTACAATCGGTCTATT+TGG + chr3_4:57874422-57874441 Msa0509420:CDS 30.0%
! CTATTTGGCTACCTTTTATT+TGG + chr3_4:57874437-57874456 Msa0509420:CDS 30.0%
GTGTTGAATCTCCAAATAAA+AGG - chr3_4:57874451-57874470 None:intergenic 30.0%
!! GAAAGAAGTACCAAATATCT+GGG - chr3_4:57874721-57874740 None:intergenic 30.0%
!! TGAAAGAAGTACCAAATATC+TGG - chr3_4:57874722-57874741 None:intergenic 30.0%
!! TAAAGCATTGTATATGTTGG+TGG - chr3_4:57875025-57875044 None:intergenic 30.0%
TAAGTAGCAAGTCATAATGT+TGG + chr3_4:57875043-57875062 Msa0509420:exon 30.0%
GAAAGCAACAACAAAAGAAT+GGG - chr3_4:57875080-57875099 None:intergenic 30.0%
AGAAAGCAACAACAAAAGAA+TGG - chr3_4:57875081-57875100 None:intergenic 30.0%
!!! CTACAAAAAGTTTTATTGGG+GGG + chr3_4:57875102-57875121 Msa0509420:three_prime_UTR 30.0%
! CTTGCAAGATTTTTACAACA+AGG - chr3_4:57875207-57875226 None:intergenic 30.0%
! CTTGTTGTAAAAATCTTGCA+AGG + chr3_4:57875205-57875224 Msa0509420:three_prime_UTR 30.0%
TTTCATTACCATCATCACAT+GGG - chr3_4:57875419-57875438 None:intergenic 30.0%
! TTTTCATTACCATCATCACA+TGG - chr3_4:57875420-57875439 None:intergenic 30.0%
!!! ATTTTTTAGGGAGTTGAGAG+AGG - chr3_4:57872865-57872884 None:intergenic 35.0%
ATAAGAATCAAGGCGAAAGA+AGG - chr3_4:57873065-57873084 None:intergenic 35.0%
! GAGCTAAATATGACACCTTT+TGG + chr3_4:57873325-57873344 Msa0509420:intron 35.0%
!! TTGAGCAAAATCAAGTTGAG+TGG + chr3_4:57873402-57873421 Msa0509420:intron 35.0%
! TTTGTGTGTGATGGAATAAC+AGG + chr3_4:57873522-57873541 Msa0509420:intron 35.0%
! TTGTGTGTGATGGAATAACA+GGG + chr3_4:57873523-57873542 Msa0509420:intron 35.0%
! ATAACAGGGGATGTTTTATC+TGG + chr3_4:57873537-57873556 Msa0509420:intron 35.0%
! GTTTTATCTGGAAAGGAAAG+TGG + chr3_4:57873549-57873568 Msa0509420:CDS 35.0%
GATTCATTGGTGGAATTCAA+GGG + chr3_4:57873611-57873630 Msa0509420:CDS 35.0%
TTAGTGCTTGTTTGGTATGA+CGG + chr3_4:57873738-57873757 Msa0509420:intron 35.0%
TATTCCAGAACTGTACTTCA+AGG - chr3_4:57874028-57874047 None:intergenic 35.0%
AATATCAACTCTTCTTGCTG+TGG + chr3_4:57874044-57874063 Msa0509420:CDS 35.0%
TAATCGAGGAACAAGTTTAG+GGG - chr3_4:57874130-57874149 None:intergenic 35.0%
CTAATCGAGGAACAAGTTTA+GGG - chr3_4:57874131-57874150 None:intergenic 35.0%
TCTAATCGAGGAACAAGTTT+AGG - chr3_4:57874132-57874151 None:intergenic 35.0%
TCTAGCAACACCCTTAAAAA+AGG - chr3_4:57874256-57874275 None:intergenic 35.0%
TTACTTGCAGTACATCCTAT+TGG + chr3_4:57874329-57874348 Msa0509420:intron 35.0%
TACTTGCAGTACATCCTATT+GGG + chr3_4:57874330-57874349 Msa0509420:intron 35.0%
!!! ACTGCTGTTTTCATATGAGA+TGG - chr3_4:57874371-57874390 None:intergenic 35.0%
GCTGTTGTCTACTTTACAAT+CGG + chr3_4:57874413-57874432 Msa0509420:CDS 35.0%
GAATAGGAGTTCATCAATGT+TGG - chr3_4:57874606-57874625 None:intergenic 35.0%
CTATAGCAATCCCAGATATT+TGG + chr3_4:57874708-57874727 Msa0509420:CDS 35.0%
TGGTACTTCTTTCAGTTTCT+AGG + chr3_4:57874728-57874747 Msa0509420:CDS 35.0%
ACCTTAAAACAATAGTGCCA+GGG - chr3_4:57874787-57874806 None:intergenic 35.0%
TACCTTAAAACAATAGTGCC+AGG - chr3_4:57874788-57874807 None:intergenic 35.0%
AATCGTGAATGAATAAAGCC+AGG + chr3_4:57874880-57874899 Msa0509420:intron 35.0%
ACAAAGAAAATTTAGGAGCC+TGG - chr3_4:57874901-57874920 None:intergenic 35.0%
ACAGGACAAAATTATAGCAC+TGG + chr3_4:57874957-57874976 Msa0509420:CDS 35.0%
! AAATTCCAGGCTCATTTTGT+TGG + chr3_4:57875147-57875166 Msa0509420:three_prime_UTR 35.0%
AATAACTAGCTAGGTTCACA+TGG - chr3_4:57875350-57875369 None:intergenic 35.0%
CACTCAAACAATAACTAGCT+AGG - chr3_4:57875359-57875378 None:intergenic 35.0%
!! GGTGTTATGAAGGAAGCTTT+TGG + chr3_4:57873161-57873180 Msa0509420:CDS 40.0%
! TGTGTGTGATGGAATAACAG+GGG + chr3_4:57873524-57873543 Msa0509420:intron 40.0%
! AGGGGATGTTTTATCTGGAA+AGG + chr3_4:57873542-57873561 Msa0509420:intron 40.0%
TTATCTGGAAAGGAAAGTGG+AGG + chr3_4:57873552-57873571 Msa0509420:CDS 40.0%
! GACTTTTGCAACAGTGGTTT+GGG + chr3_4:57873589-57873608 Msa0509420:CDS 40.0%
! AACAGTGGTTTGGGATTCAT+TGG + chr3_4:57873598-57873617 Msa0509420:CDS 40.0%
GGATTCATTGGTGGAATTCA+AGG + chr3_4:57873610-57873629 Msa0509420:CDS 40.0%
! AGTTGCTCTTTTCATCACCT+TGG + chr3_4:57873635-57873654 Msa0509420:CDS 40.0%
! AGGAAGCATAACAAGAACCA+AGG - chr3_4:57873655-57873674 None:intergenic 40.0%
! GGTTCTTGTTATGCTTCCTT+TGG + chr3_4:57873656-57873675 Msa0509420:CDS 40.0%
ACACGTTTGTACAAGACCAA+AGG - chr3_4:57873675-57873694 None:intergenic 40.0%
!! TTGGTCTTGTACAAACGTGT+AGG + chr3_4:57873675-57873694 Msa0509420:CDS 40.0%
TAGTGCTTGTTTGGTATGAC+GGG + chr3_4:57873739-57873758 Msa0509420:intron 40.0%
!!! CGTATATTTTGGTATCACGG+TGG - chr3_4:57873786-57873805 None:intergenic 40.0%
AAAGTGCAAGTTCGGAATCA+CGG - chr3_4:57873915-57873934 None:intergenic 40.0%
GAATCCTTGAAGTACAGTTC+TGG + chr3_4:57874021-57874040 Msa0509420:intron 40.0%
! CATTTGTTGCCATCTGTTCT+GGG + chr3_4:57874067-57874086 Msa0509420:CDS 40.0%
! GATGTTTGGTAGTCTAATCG+AGG - chr3_4:57874144-57874163 None:intergenic 40.0%
CTATGCACTCCACATTATGT+TGG + chr3_4:57874550-57874569 Msa0509420:CDS 40.0%
GAGGGAATACCAACATAATG+TGG - chr3_4:57874562-57874581 None:intergenic 40.0%
! CACAAATCTCTTGTTGTCTG+TGG - chr3_4:57874648-57874667 None:intergenic 40.0%
GATTGCTATAGCAGCAAGAT+AGG - chr3_4:57874699-57874718 None:intergenic 40.0%
!! TCCCTGGCACTATTGTTTTA+AGG + chr3_4:57874783-57874802 Msa0509420:CDS 40.0%
TCTTGGCATATCAACAAGAC+AGG + chr3_4:57874939-57874958 Msa0509420:CDS 40.0%
!!! AAAGTTTTATTGGGGGGAGT+TGG + chr3_4:57875108-57875127 Msa0509420:three_prime_UTR 40.0%
!!! AAGTTTTATTGGGGGGAGTT+GGG + chr3_4:57875109-57875128 Msa0509420:three_prime_UTR 40.0%
CAAAACCAACAAAATGAGCC+TGG - chr3_4:57875155-57875174 None:intergenic 40.0%
CAGAAACGTGTGACAAAGTT+GGG + chr3_4:57875280-57875299 Msa0509420:three_prime_UTR 40.0%
AGAAACGTGTGACAAAGTTG+GGG + chr3_4:57875281-57875300 Msa0509420:three_prime_UTR 40.0%
TAAAAGGTCCCATGTGATGA+TGG + chr3_4:57875408-57875427 Msa0509420:three_prime_UTR 40.0%
TCCCTAAAAAATCTCACCGC+CGG + chr3_4:57872873-57872892 Msa0509420:five_prime_UTR 45.0%
GATTTGATCCGGTCAAGAGA+GGG - chr3_4:57872932-57872951 None:intergenic 45.0%
TCTCTTGACCGGATCAAATC+CGG + chr3_4:57872932-57872951 Msa0509420:CDS 45.0%
AATGTCGCCACCAGCATAAT+CGG + chr3_4:57872990-57873009 Msa0509420:CDS 45.0%
CTCCGGAATCATGTCAATTC+CGG + chr3_4:57873013-57873032 Msa0509420:CDS 45.0%
! CTCATGCGTTTTACACATGC+CGG + chr3_4:57873119-57873138 Msa0509420:CDS 45.0%
!! GACGTACTCTGGTGTTATGA+AGG + chr3_4:57873151-57873170 Msa0509420:CDS 45.0%
!!! AAGGAAGCTTTTGGTTCCGT+TGG + chr3_4:57873170-57873189 Msa0509420:CDS 45.0%
TTGAGCGCTTAAAGCTCCAA+CGG - chr3_4:57873189-57873208 None:intergenic 45.0%
! ACTTGGGACTTTTGCAACAG+TGG + chr3_4:57873583-57873602 Msa0509420:CDS 45.0%
! GGACTTTTGCAACAGTGGTT+TGG + chr3_4:57873588-57873607 Msa0509420:CDS 45.0%
! AGTGGTTTGGGATTCATTGG+TGG + chr3_4:57873601-57873620 Msa0509420:CDS 45.0%
!! TTTGGTATGACGGGCTTTGT+TGG + chr3_4:57873748-57873767 Msa0509420:intron 45.0%
! GCATTTGTTGCCATCTGTTC+TGG + chr3_4:57874066-57874085 Msa0509420:CDS 45.0%
AATAGCCAACCCAGAACAGA+TGG - chr3_4:57874079-57874098 None:intergenic 45.0%
!! TGGGTTGGCTATTGTTGCTT+TGG + chr3_4:57874086-57874105 Msa0509420:CDS 45.0%
!! GCTATTGTTGCTTTGGTGCA+AGG + chr3_4:57874093-57874112 Msa0509420:CDS 45.0%
GTGAATGCTGTGACTACAAC+AGG - chr3_4:57874192-57874211 None:intergenic 45.0%
CTTGCAAGCTCAAACCCAAT+AGG - chr3_4:57874347-57874366 None:intergenic 45.0%
!! CAGAATGCTGATTCTGCTGT+TGG + chr3_4:57874497-57874516 Msa0509420:CDS 45.0%
ATCAACAACAGCACTATGCC+TGG + chr3_4:57874751-57874770 Msa0509420:CDS 45.0%
!!! TGCCTGGCTTTTATTTTCCC+TGG + chr3_4:57874767-57874786 Msa0509420:CDS 45.0%
TGCCAGGGAAAATAAAAGCC+AGG - chr3_4:57874772-57874791 None:intergenic 45.0%
TCTAATGACAGGGATGCTCT+TGG + chr3_4:57874922-57874941 Msa0509420:intron 45.0%
!!! AGTTTTATTGGGGGGAGTTG+GGG + chr3_4:57875110-57875129 Msa0509420:three_prime_UTR 45.0%
GCAGAAACGTGTGACAAAGT+TGG + chr3_4:57875279-57875298 Msa0509420:three_prime_UTR 45.0%
GGGAGTTGAGAGAGGAAATG+TGG - chr3_4:57872857-57872876 None:intergenic 50.0%
!!! CCCGGCGGTGAGATTTTTTA+GGG - chr3_4:57872877-57872896 None:intergenic 50.0%
CCCTAAAAAATCTCACCGCC+GGG + chr3_4:57872874-57872893 Msa0509420:five_prime_UTR 50.0%
!!! TCCCGGCGGTGAGATTTTTT+AGG - chr3_4:57872878-57872897 None:intergenic 50.0%
GGATTTGATCCGGTCAAGAG+AGG - chr3_4:57872933-57872952 None:intergenic 50.0%
! GCTGGTGGCGACATTGAATA+CGG - chr3_4:57872985-57873004 None:intergenic 50.0%
CGCCGGAATTGACATGATTC+CGG - chr3_4:57873018-57873037 None:intergenic 50.0%
! CCGAGAACTTTGAGAATCGC+CGG - chr3_4:57873035-57873054 None:intergenic 50.0%
CCGGCGATTCTCAAAGTTCT+CGG + chr3_4:57873032-57873051 Msa0509420:CDS 50.0%
CACGGCGACGATAAGAATCA+AGG - chr3_4:57873075-57873094 None:intergenic 50.0%
AACAGAAACTTCGGCGAGCA+CGG - chr3_4:57873093-57873112 None:intergenic 50.0%
AGAGTACGTCGTGGTTACAC+CGG - chr3_4:57873141-57873160 None:intergenic 50.0%
CATAACACCAGAGTACGTCG+TGG - chr3_4:57873150-57873169 None:intergenic 50.0%
AAGTGGAGGTGAAGTGCACT+TGG + chr3_4:57873566-57873585 Msa0509420:CDS 50.0%
AGTGGAGGTGAAGTGCACTT+GGG + chr3_4:57873567-57873586 Msa0509420:CDS 50.0%
TACGAAGTCCATGTTCGGCT+TGG + chr3_4:57873802-57873821 Msa0509420:intron 50.0%
ATTCACTGCCAAGCCGAACA+TGG - chr3_4:57873813-57873832 None:intergenic 50.0%
! TGTTGCCATCTGTTCTGGGT+TGG + chr3_4:57874071-57874090 Msa0509420:CDS 50.0%
GCAGAGGCTTCTTAGGGAAT+AGG - chr3_4:57874622-57874641 None:intergenic 50.0%
TGGCTAGCAGAGGCTTCTTA+GGG - chr3_4:57874628-57874647 None:intergenic 50.0%
TTGTTGTCTGTGGCTAGCAG+AGG - chr3_4:57874638-57874657 None:intergenic 50.0%
GTGGAAGGGGCAAAAATTCC+AGG + chr3_4:57875134-57875153 Msa0509420:three_prime_UTR 50.0%
!! AGACGGTGTTGGTGACATCC+CGG - chr3_4:57872895-57872914 None:intergenic 55.0%
ATGTCACCAACACCGTCTGC+CGG + chr3_4:57872897-57872916 Msa0509420:CDS 55.0%
!! CAAGAGAGGGACGTTGACTC+CGG - chr3_4:57872919-57872938 None:intergenic 55.0%
GTCAACGTCCCTCTCTTGAC+CGG + chr3_4:57872921-57872940 Msa0509420:CDS 55.0%
CTTGACCGGATCAAATCCGG+CGG + chr3_4:57872935-57872954 Msa0509420:CDS 55.0%
CGACATTGAATACGGCGCCA+GGG - chr3_4:57872977-57872996 None:intergenic 55.0%
GATTCCGGAGCCGATTATGC+TGG - chr3_4:57873003-57873022 None:intergenic 55.0%
GGTGTAACCACGACGTACTC+TGG + chr3_4:57873140-57873159 Msa0509420:CDS 55.0%
GTGGCTAGCAGAGGCTTCTT+AGG - chr3_4:57874629-57874648 None:intergenic 55.0%
TTGACTCCGGCAGACGGTGT+TGG - chr3_4:57872906-57872925 None:intergenic 60.0%
AACCGCCGCCGGATTTGATC+CGG - chr3_4:57872943-57872962 None:intergenic 60.0%
ATTGAACCAGGAACCGCCGC+CGG - chr3_4:57872954-57872973 None:intergenic 60.0%
GCGGTTCCTGGTTCAATCCC+TGG + chr3_4:57872957-57872976 Msa0509420:CDS 60.0%
GCGACATTGAATACGGCGCC+AGG - chr3_4:57872978-57872997 None:intergenic 60.0%
! GCCACCAGCATAATCGGCTC+CGG + chr3_4:57872996-57873015 Msa0509420:CDS 60.0%
TCCGGAGCCGATTATGCTGG+TGG - chr3_4:57873000-57873019 None:intergenic 60.0%
!! CGGTGTTGGTGACATCCCGG+CGG - chr3_4:57872892-57872911 None:intergenic 65.0%
!! GGGACGTTGACTCCGGCAGA+CGG - chr3_4:57872912-57872931 None:intergenic 65.0%
GACCGGATCAAATCCGGCGG+CGG + chr3_4:57872938-57872957 Msa0509420:CDS 65.0%
TCAAATCCGGCGGCGGTTCC+TGG + chr3_4:57872945-57872964 Msa0509420:CDS 65.0%
ACGGCGCCAGGGATTGAACC+AGG - chr3_4:57872966-57872985 None:intergenic 65.0%
TATTGGGGGGAGTTGGGGCG+TGG + chr3_4:57875115-57875134 Msa0509420:three_prime_UTR 65.0%
GGGGGAGTTGGGGCGTGGAA+GGG + chr3_4:57875120-57875139 Msa0509420:three_prime_UTR 70.0%
GGGGAGTTGGGGCGTGGAAG+GGG + chr3_4:57875121-57875140 Msa0509420:three_prime_UTR 70.0%
GGGGGGAGTTGGGGCGTGGA+AGG + chr3_4:57875119-57875138 Msa0509420:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 57872813 57875452 57872813 ID=Msa0509420;Name=Msa0509420
chr3_4 mRNA 57872813 57875452 57872813 ID=Msa0509420-mRNA-1;Parent=Msa0509420;Name=Msa0509420-mRNA-1;_AED=0.20;_eAED=0.20;_QI=84|1|1|1|1|1|5|393|438
chr3_4 exon 57872813 57873263 57872813 ID=Msa0509420-mRNA-1:exon:13277;Parent=Msa0509420-mRNA-1
chr3_4 exon 57873544 57873696 57873544 ID=Msa0509420-mRNA-1:exon:13278;Parent=Msa0509420-mRNA-1
chr3_4 exon 57874022 57874225 57874022 ID=Msa0509420-mRNA-1:exon:13279;Parent=Msa0509420-mRNA-1
chr3_4 exon 57874339 57874804 57874339 ID=Msa0509420-mRNA-1:exon:13280;Parent=Msa0509420-mRNA-1
chr3_4 exon 57874933 57875452 57874933 ID=Msa0509420-mRNA-1:exon:13281;Parent=Msa0509420-mRNA-1
chr3_4 five_prime_UTR 57872813 57872896 57872813 ID=Msa0509420-mRNA-1:five_prime_utr;Parent=Msa0509420-mRNA-1
chr3_4 CDS 57872897 57873263 57872897 ID=Msa0509420-mRNA-1:cds;Parent=Msa0509420-mRNA-1
chr3_4 CDS 57873544 57873696 57873544 ID=Msa0509420-mRNA-1:cds;Parent=Msa0509420-mRNA-1
chr3_4 CDS 57874022 57874225 57874022 ID=Msa0509420-mRNA-1:cds;Parent=Msa0509420-mRNA-1
chr3_4 CDS 57874339 57874804 57874339 ID=Msa0509420-mRNA-1:cds;Parent=Msa0509420-mRNA-1
chr3_4 CDS 57874933 57875059 57874933 ID=Msa0509420-mRNA-1:cds;Parent=Msa0509420-mRNA-1
chr3_4 three_prime_UTR 57875060 57875452 57875060 ID=Msa0509420-mRNA-1:three_prime_utr;Parent=Msa0509420-mRNA-1
Gene Sequence

>Msa0509420

ATGTCACCAACACCGTCTGCCGGAGTCAACGTCCCTCTCTTGACCGGATCAAATCCGGCGGCGGTTCCTGGTTCAATCCCTGGCGCCGTATTCAATGTCGCCACCAGCATAATCGGCTCCGGAATCATGTCAATTCCGGCGATTCTCAAAGTTCTCGGCGTGCTTCCTTCTTTCGCCTTGATTCTTATCGTCGCCGTGCTCGCCGAAGTTTCTGTTGATTTTCTCATGCGTTTTACACATGCCGGTGTAACCACGACGTACTCTGGTGTTATGAAGGAAGCTTTTGGTTCCGTTGGAGCTTTAAGCGCTCAAGTTTGTGTTATAATCACAAACTTTGGGGGTTTAGTTCTCTTCCTAATTATCATTGGGGATGTTTTATCTGGAAAGGAAAGTGGAGGTGAAGTGCACTTGGGACTTTTGCAACAGTGGTTTGGGATTCATTGGTGGAATTCAAGGGAAGTTGCTCTTTTCATCACCTTGGTTCTTGTTATGCTTCCTTTGGTCTTGTACAAACGTGTAGAATCCTTGAAGTACAGTTCTGGAATATCAACTCTTCTTGCTGTGGCATTTGTTGCCATCTGTTCTGGGTTGGCTATTGTTGCTTTGGTGCAAGGAAAAACACAAACCCCTAAACTTGTTCCTCGATTAGACTACCAAACATCATTCTTTGATTTGTTCACTGCAGTTCCTGTTGTAGTCACAGCATTCACATTTCATTTCAATGTACATCCTATTGGGTTTGAGCTTGCAAGTCCATCTCATATGAAAACAGCAGTTCGATTAGCAATAATGTTTTGTGCTGTTGTCTACTTTACAATCGGTCTATTTGGCTACCTTTTATTTGGAGATTCAACACAATCAGACATTCTAATCAATTTCGATCAGAATGCTGATTCTGCTGTTGGTTCCTTGTTCAATAGTTTAATACGTGTAAGCTATGCACTCCACATTATGTTGGTATTCCCTCTTATAAATTTCTCTTTGAGAGCCAACATTGATGAACTCCTATTCCCTAAGAAGCCTCTGCTAGCCACAGACAACAAGAGATTTGTGATTATCACTCTTGTGCTCTTAGTTTTCTCCTATCTTGCTGCTATAGCAATCCCAGATATTTGGTACTTCTTTCAGTTTCTAGGATCAACAACAGCACTATGCCTGGCTTTTATTTTCCCTGGCACTATTGTTTTAAGGGATGCTCTTGGCATATCAACAAGACAGGACAAAATTATAGCACTGGTTATGATTATACTAGCTGTAGTGACAAGTGTAATTGCAATCTCCACCAACATATACAATGCTTTAAGTAGCAAGTCATAA
Protein sequence

>Msa0509420

MSPTPSAGVNVPLLTGSNPAAVPGSIPGAVFNVATSIIGSGIMSIPAILKVLGVLPSFALILIVAVLAEVSVDFLMRFTHAGVTTTYSGVMKEAFGSVGALSAQVCVIITNFGGLVLFLIIIGDVLSGKESGGEVHLGLLQQWFGIHWWNSREVALFITLVLVMLPLVLYKRVESLKYSSGISTLLAVAFVAICSGLAIVALVQGKTQTPKLVPRLDYQTSFFDLFTAVPVVVTAFTFHFNVHPIGFELASPSHMKTAVRLAIMFCAVVYFTIGLFGYLLFGDSTQSDILINFDQNADSAVGSLFNSLIRVSYALHIMLVFPLINFSLRANIDELLFPKKPLLATDNKRFVIITLVLLVFSYLAAIAIPDIWYFFQFLGSTTALCLAFIFPGTIVLRDALGISTRQDKIIALVMIILAVVTSVIAISTNIYNALSSKS*