AlfalfaGEDB | Msa1304980

M. sativa cultivar zhongmu-4 / Msa1304980

KEGG: K08726
GO: GO:0000287 GO:0001676 GO:0003008 GO:0003013 GO:0003018 GO:0003674 GO:0003824 GO:0004301 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006633 GO:0006690 GO:0006725 GO:0006793 GO:0006796 GO:0006805 GO:0006810 GO:0006873 GO:0006874 GO:0006875 GO:0006886 GO:0006950 GO:0006952 GO:0006954 GO:0006996 GO:0007031 GO:0008015 GO:0008104 GO:0008150 GO:0008152 GO:0008217 GO:0008610 GO:0009056 GO:0009058 GO:0009410 GO:0009636 GO:0009810 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015031 GO:0015643 GO:0015833 GO:0016043 GO:0016053 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0016801 GO:0016803 GO:0017144 GO:0018904 GO:0019216 GO:0019218 GO:0019222 GO:0019369 GO:0019373 GO:0019439 GO:0019725 GO:0019752 GO:0030003 GO:0030258 GO:0031907 GO:0031974 GO:0032501 GO:0032787 GO:0033036 GO:0033365 GO:0033559 GO:0034613 GO:0035150 GO:0035296 GO:0042221 GO:0042577 GO:0042578 GO:0042579 GO:0042592 GO:0042632 GO:0042759 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046272 GO:0046394 GO:0046483 GO:0046839 GO:0046872 GO:0046907 GO:0046983 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050880 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055088 GO:0055092 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070887 GO:0071466 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072330 GO:0072503 GO:0072507 GO:0072593 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0090066 GO:0090181 GO:0097176 GO:0097746 GO:0097755 GO:0098771 GO:1900673 GO:1901360 GO:1901361 GO:1901568 GO:1901575 GO:1901576
PFAM: PF00561.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1304980	sp\|I6YGS0\|EPHA_MYCTU	34.462	325	159	7	291	570	1	316	2.17e-48	174
Msa1304980	sp\|I6YGS0\|EPHA_MYCTU	36.032	247	140	7	1	233	1	243	1.38e-45	166

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1304980	A0A3Q0FD40	60.366	656	175	12	1	573	1	654	0.0	763

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0025060	Msa1304980	-0.846916	1.608329e-59	-8.615850e-47
Msa0025090	Msa1304980	-0.801639	8.189298e-49	-8.615850e-47
Msa0030320	Msa1304980	-0.804047	2.605031e-49	-8.615850e-47
Msa0030710	Msa1304980	-0.802770	4.790837e-49	-8.615850e-47
Msa0034800	Msa1304980	-0.818176	2.246543e-52	-8.615850e-47
Msa0038910	Msa1304980	-0.813492	2.490950e-51	-8.615850e-47
Msa0046680	Msa1304980	0.812978	3.229203e-51	-8.615850e-47
Msa0050850	Msa1304980	-0.808308	3.300878e-50	-8.615850e-47
Msa0077920	Msa1304980	-0.800123	1.670528e-48	-8.615850e-47
Msa0081700	Msa1304980	-0.816295	5.953982e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1304980	MtrunA17_Chr7g0272481	84.639	319	13	2	291	573	1	319	0.0	542
Msa1304980	MtrunA17_Chr7g0272481	79.297	256	43	2	1	252	1	250	6.45e-147	426
Msa1304980	MtrunA17_Chr7g0272171	82.132	319	21	2	291	573	1	319	0.0	531
Msa1304980	MtrunA17_Chr7g0272171	84.766	256	29	2	1	252	1	250	4.73e-155	446
Msa1304980	MtrunA17_Chr7g0272531	80.124	322	28	2	288	573	28	349	0.0	530
Msa1304980	MtrunA17_Chr7g0272531	86.328	256	25	2	1	252	31	280	9.70e-159	457
Msa1304980	MtrunA17_Chr7g0272501	80.251	319	27	2	291	573	1	319	0.0	521
Msa1304980	MtrunA17_Chr7g0272501	89.844	256	16	2	1	252	1	250	4.32e-165	472
Msa1304980	MtrunA17_Chr7g0272181	79.624	319	29	2	291	573	1	319	0.0	514
Msa1304980	MtrunA17_Chr7g0272181	80.859	256	39	2	1	252	1	250	6.61e-149	431

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1304980	AT3G05600.1	57.099	324	98	6	291	573	1	324	6.93e-124	368
Msa1304980	AT3G05600.1	61.157	242	85	5	1	233	1	242	3.22e-100	307
Msa1304980	AT4G02340.1	51.402	321	113	6	291	573	1	316	2.96e-109	330
Msa1304980	AT4G02340.1	54.086	257	103	5	1	252	1	247	5.41e-92	286
Msa1304980	AT4G15960.1	48.214	336	131	5	280	573	40	374	5.45e-102	313
Msa1304980	AT4G15960.1	49.434	265	117	5	1	255	51	308	1.40e-82	263
Msa1304980	AT3G05600.2	61.157	242	85	5	1	233	1	242	4.30e-101	306
Msa1304980	AT3G05600.2	55.556	243	67	6	291	492	1	243	2.70e-82	258
Msa1304980	AT2G26740.1	53.727	322	106	6	294	573	1	321	2.85e-97	299
Msa1304980	AT2G26740.1	60.944	233	80	5	4	226	1	232	4.72e-88	275

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Find 173 sgRNAs with CRISPR-Local

Find 777 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCATATTGCTGAAAA+AGG	0.266560	8_3:+68263969	Msa1304980:CDS
AAAATGCATATTGCTGAGAA+AGG	0.485302	8_3:+68271566	Msa1304980:CDS
AAACTATCGACCATGTGCAA+TGG	0.571413	8_3:-68273184	None:intergenic
AACATGAAGGAGTATATCCA+TGG	0.620148	8_3:+68266298	Msa1304980:CDS
AACTCTCCCTTGGGAAATAT+TGG	0.280214	8_3:-68265716	None:intergenic
AACTCTCCCTTGGGAAGTAT+TGG	0.434048	8_3:-68272495	None:intergenic
AACTTATTCTAGATGATTAT+TGG	0.178759	8_3:-68273093	None:intergenic
AAGGAGTATATCCATGGTGG+AGG	0.581533	8_3:+68272888	Msa1304980:CDS
AAGGAGTATATCCATGGTGG+GGG	0.600787	8_3:+68266304	Msa1304980:CDS
AAGTTGTGTAATTTGTTATT+TGG	0.252164	8_3:+68273376	Msa1304980:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGCTGAAAAAGGAAAAGA+AGG	+	chr8_3:68263978-68263997	Msa1304980:CDS	30.0%
TGTTGATCAAGTATTTCTTG+TGG	+	chr8_3:68264202-68264221	Msa1304980:CDS	30.0%
GGGATTGCTTAAAGTAAATT+AGG	-	chr8_3:68264446-68264465	None:intergenic	30.0%
ATCAAGTGTTAAATCCACTT+GGG	-	chr8_3:68265202-68265221	None:intergenic	30.0%
AATCAAGTGTTAAATCCACT+TGG	-	chr8_3:68265203-68265222	None:intergenic	30.0%
AGTAGAGTCTAAATCAAAAG+AGG	+	chr8_3:68265237-68265256	Msa1304980:intron	30.0%
ATTTGGAGACAAAATAAGCA+AGG	-	chr8_3:68265802-68265821	None:intergenic	30.0%
CAACATGACAATAATCTATG+CGG	+	chr8_3:68265955-68265974	Msa1304980:intron	30.0%
ATAATCACTTCTTCCAGATT+GGG	-	chr8_3:68266346-68266365	None:intergenic	30.0%
GAAGAAGTGATTATCCAAAA+AGG	+	chr8_3:68266352-68266371	Msa1304980:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	68263927	68273399	68263927	ID=Msa1304980;Name=Msa1304980
chr8_3	mRNA	68263927	68273399	68263927	ID=Msa1304980-mRNA-1;Parent=Msa1304980;Name=Msa1304980-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0\|0.28\|0.37\|1\|0.28\|0.5\|8\|365\|573
chr8_3	exon	68263927	68264155	68263927	ID=Msa1304980-mRNA-1:exon:21103;Parent=Msa1304980-mRNA-1
chr8_3	exon	68264168	68264394	68264168	ID=Msa1304980-mRNA-1:exon:21104;Parent=Msa1304980-mRNA-1
chr8_3	exon	68265629	68265873	68265629	ID=Msa1304980-mRNA-1:exon:21105;Parent=Msa1304980-mRNA-1
chr8_3	exon	68266278	68266415	68266278	ID=Msa1304980-mRNA-1:exon:21106;Parent=Msa1304980-mRNA-1
chr8_3	exon	68271493	68271713	68271493	ID=Msa1304980-mRNA-1:exon:21107;Parent=Msa1304980-mRNA-1
chr8_3	exon	68271768	68271937	68271768	ID=Msa1304980-mRNA-1:exon:21108;Parent=Msa1304980-mRNA-1
chr8_3	exon	68272408	68272652	68272408	ID=Msa1304980-mRNA-1:exon:21109;Parent=Msa1304980-mRNA-1
chr8_3	exon	68272788	68273399	68272788	ID=Msa1304980-mRNA-1:exon:21110;Parent=Msa1304980-mRNA-1
chr8_3	CDS	68263927	68264155	68263927	ID=Msa1304980-mRNA-1:cds;Parent=Msa1304980-mRNA-1
chr8_3	CDS	68264168	68264394	68264168	ID=Msa1304980-mRNA-1:cds;Parent=Msa1304980-mRNA-1
chr8_3	CDS	68265629	68265873	68265629	ID=Msa1304980-mRNA-1:cds;Parent=Msa1304980-mRNA-1
chr8_3	CDS	68266278	68266415	68266278	ID=Msa1304980-mRNA-1:cds;Parent=Msa1304980-mRNA-1
chr8_3	CDS	68271493	68271713	68271493	ID=Msa1304980-mRNA-1:cds;Parent=Msa1304980-mRNA-1
chr8_3	CDS	68271768	68271937	68271768	ID=Msa1304980-mRNA-1:cds;Parent=Msa1304980-mRNA-1
chr8_3	CDS	68272408	68272652	68272408	ID=Msa1304980-mRNA-1:cds;Parent=Msa1304980-mRNA-1
chr8_3	CDS	68272788	68273034	68272788	ID=Msa1304980-mRNA-1:cds;Parent=Msa1304980-mRNA-1
chr8_3	three_prime_UTR	68273035	68273399	68273035	ID=Msa1304980-mRNA-1:three_prime_utr;Parent=Msa1304980-mRNA-1

Gene Sequence

>Msa1304980

ATGGAAGGAATAGAACACAGAACAGTGGAAGTAAATGGAATCAAAATGCATATTGCTGAAAAAGGAAAAGAAGGTCCTCTTGTCTTGTTCCTTCATGGCTTCCCCGAACTGTGGTATTCATGGCGCCATCAGATTGTAGCTCTCAGCTCCCTCGGCTACCGTGCTGTTGCTCCGGATCTCCGTGGTTATGGTGACACCGATGCTCCGTCTTCAGTTAGTAGCTACACAGTAGGTGACCTTATTGCACTTATAGATTTTCTGGGTGTTGATCAAGTATTTCTTGTGGCTCATGATTGGGGTGCCATCATTGGTTGGTATCTTTGTATGTTTCGCCCTGAAAGAATCAAAGCTTATGTTTGTCTCAGTGTTCCACTACTCCGTAGAAACCCCAAAATCAGGACCGTTGATGCCATGCATGCTGCTTATGGAGATGATTATTACATATGTAGATTTCAGGAACCAGGCAAAATGGAAGCTGAGATGGCTGAAGTTGGCACTGCATATGTGATGAAAAGTACCCTCACAACTCGGAAAACTGGTCCTCCAATATTTCCCAAGGGAGAGTTTGGAACTGGATTCAACTCGGATACTCCTGACACCTTACCCTCTTGGCTTACAGAAGAAGACCTTGCTTATTTTGTCTCCAAATTTGAGAAAACAGGCTTCACTGCAGCATTGAACTACTATAGAAACTTGAACTCACAGTGTATACACCTCACTAAACATGAAGGAGTATATCCATGGTGGGGGATTCAAGGAAGATGTGCCCAATCTGGAAGAAGTGATTATCCAAAAAGGAGTGGCGCACTTCAACAATCAAGAAGCAGCAGAGGAAATAATTCACAACACAATTCAAACACAAAAAAGACGATGGAAGGAATAGAACACAGAACAGTGGAAGTGAATGGAATCAAAATGCATATTGCTGAGAAAGGAAAAGAAGGACCTGTTGTCCTGTTCCTTCACGGCTTTCCTGAACTCTGGTACTCTTGGCGCCACCAGATTGCTGCTCTCGGCTCTCTCGGATACCGTGCTGTTGCTCCAGATCTTCGTGGCTACGGTGACATCGTTGCTCTCATCGACTTGCTTGGTGTGGAACAAGTGTTTCTTGTAGCTCATGATTGGGGTGCCCTAATTGGTTGGTATCTTTGCATGTTTCGCCCTGAAAGAATCAAAGCCTATGTTTGTCTCAGTGTTCCATTTACGCGTAGAAACCCTAAAATCAGAACTGAACCTGGCAAAATAGAAGCTGAGATAGCTCAGGTTGGCACTGCATATGTGCTGAAAAATGTCCTCACAACTCGCAAAACTGGTCCTCCAATACTTCCCAAGGGAGAGTTTGGAACTGGATTCAATCCGGATACCCCTGACACCTTGCCCACTTGGCTCACAGAAGATGATCTTGCTTATTTTGTCTCCAAATTTGAGAAAACTGGCTTCACCGGAGGATTGAATTACTATAGAAATTTTAACTTAAATTGGGAGCTCACAGCACCATGGAGTGGAGTGAAAATCAAGGTGCCTGTGAAATTCATTACAGGTGATTTGGATATGGTGTACACCTCGCCACATGTGAAGGAGTATATCCATGGTGGAGGATTCAAGGAAGATGTGCCGAATTTGGAGGAAGTGATTGTGCAAAAAGGAGTAGCTCATTTCAACAATCAAGAAGCTGCAGGGGAAATCAGTAATCACATTTACGAATTCATCAAGAAATTTTGA

Protein sequence

>Msa1304980

MEGIEHRTVEVNGIKMHIAEKGKEGPLVLFLHGFPELWYSWRHQIVALSSLGYRAVAPDLRGYGDTDAPSSVSSYTVGDLIALIDFLGVDQVFLVAHDWGAIIGWYLCMFRPERIKAYVCLSVPLLRRNPKIRTVDAMHAAYGDDYYICRFQEPGKMEAEMAEVGTAYVMKSTLTTRKTGPPIFPKGEFGTGFNSDTPDTLPSWLTEEDLAYFVSKFEKTGFTAALNYYRNLNSQCIHLTKHEGVYPWWGIQGRCAQSGRSDYPKRSGALQQSRSSRGNNSQHNSNTKKTMEGIEHRTVEVNGIKMHIAEKGKEGPVVLFLHGFPELWYSWRHQIAALGSLGYRAVAPDLRGYGDIVALIDLLGVEQVFLVAHDWGALIGWYLCMFRPERIKAYVCLSVPFTRRNPKIRTEPGKIEAEIAQVGTAYVLKNVLTTRKTGPPILPKGEFGTGFNPDTPDTLPTWLTEDDLAYFVSKFEKTGFTGGLNYYRNFNLNWELTAPWSGVKIKVPVKFITGDLDMVYTSPHVKEYIHGGGFKEDVPNLEEVIVQKGVAHFNNQEAAGEISNHIYEFIKKF*