AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0579720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0579720 MtrunA17_Chr5g0394561 84.553 123 14 1 1 118 1 123 3.69e-69 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 85 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTTGTTAAGAATGAATT+AGG 0.250316 4_1:+84577609 Msa0579720:three_prime_UTR
TGATGAATTTAGTACCCTTT+TGG 0.283432 4_1:-84575210 None:intergenic
TCAGAGAAAATGATAGTAAT+TGG 0.286768 4_1:-84576814 None:intergenic
AATAATTATTCTTTGTTAGT+AGG 0.294269 4_1:+84577266 Msa0579720:three_prime_UTR
GTAGAAACTAGCCTATTAAA+TGG 0.295916 4_1:+84577137 Msa0579720:three_prime_UTR
TCTCTTTCTCTTAAACTTGC+TGG 0.314804 4_1:-84576894 None:intergenic
TTCCTTATCTTACTTGCAAC+AGG 0.317413 4_1:-84577186 None:intergenic
CTTCATTGTGTTGCCTTTGA+AGG 0.318291 4_1:+84577417 Msa0579720:three_prime_UTR
CGCTGATCGCTAGTTTGTTT+AGG 0.326950 4_1:-84575121 None:intergenic
AGCGAAGCAGGCAGGCAGGC+AGG 0.340296 4_1:+84575140 Msa0579720:five_prime_UTR
ATTCACTTGATGAACACTTT+TGG 0.345424 4_1:+84576776 Msa0579720:CDS
ATCACTCCAATCCATTTAAT+AGG 0.345839 4_1:-84577148 None:intergenic
AGATGGAGATGTAGAGAGTA+TGG 0.346250 4_1:+84576947 Msa0579720:three_prime_UTR
AAACTGCATCTTTAACAAAC+TGG 0.356424 4_1:-84577592 None:intergenic
AACAAACTGGAAACTGTAGA+TGG 0.360982 4_1:-84577579 None:intergenic
AACAAACTGGAAACTGTAGA+TGG 0.360982 4_1:-84577690 None:intergenic
TGAAGAATGTTTACCTCATC+AGG 0.361386 4_1:-84575624 None:intergenic
CAGCTGCATCTTTAACAAAC+TGG 0.373302 4_1:-84577703 None:intergenic
CCTTACAAAACCGGCTTGTA+AGG 0.389747 4_1:-84577488 None:intergenic
AGGCAGGCAGGCAACGTAAT+GGG 0.394986 4_1:+84575152 Msa0579720:exon
GCTCATTCACAAATTTGAAA+TGG 0.398125 4_1:+84576864 Msa0579720:three_prime_UTR
AACAACTTCTCATTTCCAAA+AGG 0.412565 4_1:+84575195 Msa0579720:CDS
TGCAGATCACATTCTTTCAC+TGG 0.418252 4_1:+84576696 Msa0579720:CDS
GATCAGCGAAGCAGGCAGGC+AGG 0.419897 4_1:+84575136 Msa0579720:five_prime_UTR
AACTAGCCTATTAAATGGAT+TGG 0.433028 4_1:+84577142 Msa0579720:three_prime_UTR
TTCATTGTGTTGCCTTTGAA+GGG 0.441300 4_1:+84577418 Msa0579720:three_prime_UTR
AATTTCTCTCACCTGCTGTA+AGG 0.454722 4_1:-84576544 None:intergenic
TCGGGTGCTGCTGCATGGAC+AGG 0.461240 4_1:+84575696 Msa0579720:CDS
CTGACTCATCCTTACAAAAC+CGG 0.462771 4_1:-84577497 None:intergenic
CAAGTTTCTGTTAGCAATGT+GGG 0.470547 4_1:-84577452 None:intergenic
CAACACAATGAAGGGTTTGA+AGG 0.475112 4_1:-84577407 None:intergenic
CAGGCAGGCAGGCAACGTAA+TGG 0.476197 4_1:+84575151 Msa0579720:exon
GGGTTTGAAGGCTGCAATGA+AGG 0.483149 4_1:-84577395 None:intergenic
TTCTCTTAAACTTGCTGGAG+AGG 0.485869 4_1:-84576889 None:intergenic
CTTCAAAGGCAACACAATGA+AGG 0.490759 4_1:-84577416 None:intergenic
TATTTAACCTACGAAGGGGT+TGG 0.493546 4_1:-84576986 None:intergenic
ACAACTTCTCATTTCCAAAA+GGG 0.495370 4_1:+84575196 Msa0579720:CDS
ATGCCGCGATTAAGCGAGTA+GGG 0.497323 4_1:-84577359 None:intergenic
AGCAATATAGAGACTGTAGA+TGG 0.497945 4_1:+84576930 Msa0579720:three_prime_UTR
AAATATCCTCAGTCCACAAA+AGG 0.500662 4_1:-84576653 None:intergenic
AAACTAGCGATCAGCGAAGC+AGG 0.508176 4_1:+84575128 Msa0579720:five_prime_UTR
AAGTAAGATAAGGAAACCTT+AGG 0.510580 4_1:+84577194 Msa0579720:three_prime_UTR
CTTACTGTGTTCTGTACCAT+AGG 0.510901 4_1:+84577038 Msa0579720:three_prime_UTR
TTGTTTCGGGTGCTGCTGCA+TGG 0.518260 4_1:+84575691 Msa0579720:CDS
TTGTGTTGCCTTTGAAGGGG+TGG 0.519382 4_1:+84577422 Msa0579720:three_prime_UTR
TTTCAAGTTAACCTTACAGC+AGG 0.521889 4_1:+84576533 Msa0579720:CDS
GACTAAATCCACCCCTTCAA+AGG 0.525477 4_1:-84577430 None:intergenic
ATTTGTTATAATACGTACTC+AGG 0.537041 4_1:-84576841 None:intergenic
TAGCGATCAGCGAAGCAGGC+AGG 0.541579 4_1:+84575132 Msa0579720:five_prime_UTR
ACCCTGTTGCAAGTAAGATA+AGG 0.544458 4_1:+84577184 Msa0579720:three_prime_UTR
ACGGCAGTAACTGGGAGACT+TGG 0.544989 4_1:+84576503 Msa0579720:intron
TTCAAAGGCAACACAATGAA+GGG 0.546711 4_1:-84577415 None:intergenic
ACAAACTGGAAACTGTAGAT+GGG 0.551430 4_1:-84577578 None:intergenic
ACAAACTGGAAACTGTAGAT+GGG 0.551430 4_1:-84577689 None:intergenic
CAATATAAAATCTGATAGCA+TGG 0.555571 4_1:+84577634 Msa0579720:three_prime_UTR
GTTCCTTTACGGCAGTAACT+GGG 0.559706 4_1:+84576495 Msa0579720:intron
ACTCTACCAAGTGCGTCCTA+AGG 0.560325 4_1:-84577210 None:intergenic
TGGAAATTCTGCCTTCATTG+TGG 0.561423 4_1:+84577331 Msa0579720:three_prime_UTR
GTAGGGGCGGACCACAATGA+AGG 0.564894 4_1:-84577342 None:intergenic
AGCCCTCTCACCTTACAAGC+CGG 0.566268 4_1:+84577478 Msa0579720:three_prime_UTR
GGCTCTATTTAACCTACGAA+GGG 0.578290 4_1:-84576991 None:intergenic
CGCGATTAAGCGAGTAGGGG+CGG 0.579817 4_1:-84577355 None:intergenic
GACATTGATTTGTCACACAA+AGG 0.582985 4_1:-84577088 None:intergenic
GTGATCTGCAGAAGAATACA+AGG 0.587623 4_1:-84576683 None:intergenic
TTCACTTCAAATAAACGTCT+AGG 0.589744 4_1:-84577291 None:intergenic
TCCTTATCTTACTTGCAACA+GGG 0.596546 4_1:-84577185 None:intergenic
AACCGGCTTGTAAGGTGAGA+GGG 0.600933 4_1:-84577480 None:intergenic
AGGAAACCTTAGGACGCACT+TGG 0.603736 4_1:+84577204 Msa0579720:three_prime_UTR
AGAGTATGGAATGAATGTAG+AGG 0.609023 4_1:+84576961 Msa0579720:three_prime_UTR
GGCAAAATTGACACCTGATG+AGG 0.610013 4_1:+84575611 Msa0579720:intron
GAATTGAGAACAACATGTGT+TGG 0.611077 4_1:+84577311 Msa0579720:three_prime_UTR
AATGCCGCGATTAAGCGAGT+AGG 0.613675 4_1:-84577360 None:intergenic
TCATTGTGTTGCCTTTGAAG+GGG 0.617589 4_1:+84577419 Msa0579720:three_prime_UTR
TCAAGTTTCTGTTAGCAATG+TGG 0.617853 4_1:-84577453 None:intergenic
GATCACATTCTTTCACTGGA+CGG 0.618350 4_1:+84576700 Msa0579720:CDS
TGGCTCTATTTAACCTACGA+AGG 0.619644 4_1:-84576992 None:intergenic
AAACCGGCTTGTAAGGTGAG+AGG 0.623481 4_1:-84577481 None:intergenic
CAAACTGGAAACTGTAGATG+GGG 0.623862 4_1:-84577577 None:intergenic
CAAACTGGAAACTGTAGATG+GGG 0.623862 4_1:-84577688 None:intergenic
ACTGTGTTCTGTACCATAGG+TGG 0.624270 4_1:+84577041 Msa0579720:three_prime_UTR
GGACGGCAGTACATTACAGA+AGG 0.634546 4_1:+84576717 Msa0579720:CDS
AGAGGATCCAACCCCTTCGT+AGG 0.638072 4_1:+84576979 Msa0579720:three_prime_UTR
CGCCCCTACTCGCTTAATCG+CGG 0.640895 4_1:+84577356 Msa0579720:three_prime_UTR
TGCCGCGATTAAGCGAGTAG+GGG 0.697980 4_1:-84577358 None:intergenic
GCTCTATTTAACCTACGAAG+GGG 0.698435 4_1:-84576990 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAACAGCTTATATTTTAT+AGG + chr4_1:84575437-84575456 Msa0579720:intron 15.0%
!! CAAATAGAATAAAGTTAAAT+TGG - chr4_1:84575466-84575485 None:intergenic 15.0%
!! GAAATAAGATTAATTGTAAA+AGG - chr4_1:84575748-84575767 None:intergenic 15.0%
!! AATAATTATTCTTTGTTAGT+AGG + chr4_1:84577266-84577285 Msa0579720:three_prime_UTR 15.0%
!!! TACAATTAATCTTATTTCCA+TGG + chr4_1:84575750-84575769 Msa0579720:intron 20.0%
!! TGAATTTAAGAGATAAGTAT+TGG - chr4_1:84576090-84576109 None:intergenic 20.0%
!!! ATGCTATCAGATTTTATATT+GGG - chr4_1:84577524-84577543 None:intergenic 20.0%
!!! ATGCTATCAGATTTTATATT+GGG - chr4_1:84577524-84577543 None:intergenic 20.0%
! AGAAATTAGAAACAAAACAG+TGG - chr4_1:84575250-84575269 None:intergenic 25.0%
! CATACTCAAATATGATAAGA+AGG - chr4_1:84575324-84575343 None:intergenic 25.0%
! TTTGAGTATGAATTTGATCA+TGG + chr4_1:84575334-84575353 Msa0579720:intron 25.0%
! TTGAGTATGAATTTGATCAT+GGG + chr4_1:84575335-84575354 Msa0579720:intron 25.0%
!!! TTGTGTATAAGCTTTTTCTA+TGG - chr4_1:84575492-84575511 None:intergenic 25.0%
!! TTTTTAACATAAGCTCTGTT+TGG - chr4_1:84575539-84575558 None:intergenic 25.0%
! GTAGTTTATGTAAATCTAAC+TGG + chr4_1:84575818-84575837 Msa0579720:intron 25.0%
! AAGATACAAAATGCAATAAC+CGG - chr4_1:84575888-84575907 None:intergenic 25.0%
! TAAATTCATCCACTTTAAGT+TGG + chr4_1:84576102-84576121 Msa0579720:intron 25.0%
! TCTTGAATGTCAATGATAAT+AGG + chr4_1:84576133-84576152 Msa0579720:intron 25.0%
! AGGAAAAGAAATCAACTATA+TGG - chr4_1:84576433-84576452 None:intergenic 25.0%
! TGATAATCATTGTTCCTTTA+CGG + chr4_1:84576484-84576503 Msa0579720:intron 25.0%
! AAAACAACAAACATGAAGAA+TGG - chr4_1:84576612-84576631 None:intergenic 25.0%
!!! TTGTTGTTTTATCATTTTGC+AGG + chr4_1:84576622-84576641 Msa0579720:intron 25.0%
! TCAGAGAAAATGATAGTAAT+TGG - chr4_1:84576817-84576836 None:intergenic 25.0%
!!! ATTTGTTATAATACGTACTC+AGG - chr4_1:84576844-84576863 None:intergenic 25.0%
!!! CCATTGTAGTTTTATTTTTG+TGG - chr4_1:84577067-84577086 None:intergenic 25.0%
! CCACAAAAATAAAACTACAA+TGG + chr4_1:84577064-84577083 Msa0579720:three_prime_UTR 25.0%
!!! AATGATGAGTTTTATGAATG+AGG + chr4_1:84577242-84577261 Msa0579720:three_prime_UTR 25.0%
!! CATGCTATCAGATTTTATAT+TGG - chr4_1:84577525-84577544 None:intergenic 25.0%
!! GTCAAATTTTGCAATTCAAA+TGG - chr4_1:84577558-84577577 None:intergenic 25.0%
!!! CAGTTTGTTAAGAATGAATT+AGG + chr4_1:84577609-84577628 Msa0579720:three_prime_UTR 25.0%
!! CATGCTATCAGATTTTATAT+TGG - chr4_1:84577525-84577544 None:intergenic 25.0%
! CAATATAAAATCTGATAGCA+TGG + chr4_1:84577523-84577542 Msa0579720:three_prime_UTR 25.0%
AACAACTTCTCATTTCCAAA+AGG + chr4_1:84575195-84575214 Msa0579720:CDS 30.0%
ACAACTTCTCATTTCCAAAA+GGG + chr4_1:84575196-84575215 Msa0579720:CDS 30.0%
! TGATGAATTTAGTACCCTTT+TGG - chr4_1:84575213-84575232 None:intergenic 30.0%
! GAAGAATGTCATAAGCTTTT+TGG - chr4_1:84575368-84575387 None:intergenic 30.0%
!!! GTTCTGACTCTTTATTTTCT+AGG + chr4_1:84575590-84575609 Msa0579720:intron 30.0%
!!! CACTCTTTTTTCACTTGTTT+CGG + chr4_1:84575677-84575696 Msa0579720:CDS 30.0%
!!! ACTCTTTTTTCACTTGTTTC+GGG + chr4_1:84575678-84575697 Msa0579720:CDS 30.0%
! TGCATACAATTTCCTGATTT+TGG + chr4_1:84575773-84575792 Msa0579720:intron 30.0%
AGATAACATGTTCCAAAATC+AGG - chr4_1:84575788-84575807 None:intergenic 30.0%
ATGTAAATCTAACTGGTAAC+TGG + chr4_1:84575825-84575844 Msa0579720:intron 30.0%
GATCATAGTTGCATGAATTT+AGG + chr4_1:84576033-84576052 Msa0579720:intron 30.0%
ATACAATACGAAGAAGAGAA+AGG + chr4_1:84576061-84576080 Msa0579720:intron 30.0%
AGAATCAAACCAACTTAAAG+TGG - chr4_1:84576114-84576133 None:intergenic 30.0%
ATTCTTTCCCAATTCTAGAT+GGG + chr4_1:84576172-84576191 Msa0579720:intron 30.0%
AATCTCAATGAGAAGATACT+TGG - chr4_1:84576236-84576255 None:intergenic 30.0%
TCATGATGTTAAGGTAATAC+AGG - chr4_1:84576304-84576323 None:intergenic 30.0%
ACGATAACATCATGATGTTA+AGG - chr4_1:84576313-84576332 None:intergenic 30.0%
! ATTCACTTGATGAACACTTT+TGG + chr4_1:84576776-84576795 Msa0579720:CDS 30.0%
GCTCATTCACAAATTTGAAA+TGG + chr4_1:84576864-84576883 Msa0579720:three_prime_UTR 30.0%
ACACAGTAAGCAGTAAAAAA+AGG - chr4_1:84577028-84577047 None:intergenic 30.0%
! GTAGAAACTAGCCTATTAAA+TGG + chr4_1:84577137-84577156 Msa0579720:three_prime_UTR 30.0%
! AACTAGCCTATTAAATGGAT+TGG + chr4_1:84577142-84577161 Msa0579720:three_prime_UTR 30.0%
ATCACTCCAATCCATTTAAT+AGG - chr4_1:84577151-84577170 None:intergenic 30.0%
AAGTAAGATAAGGAAACCTT+AGG + chr4_1:84577194-84577213 Msa0579720:three_prime_UTR 30.0%
TTCACTTCAAATAAACGTCT+AGG - chr4_1:84577294-84577313 None:intergenic 30.0%
AAACTGCATCTTTAACAAAC+TGG - chr4_1:84577595-84577614 None:intergenic 30.0%
! GTCATGTTTTGCAATTCAAA+TGG - chr4_1:84577669-84577688 None:intergenic 30.0%
TGAAGAATGTTTACCTCATC+AGG - chr4_1:84575627-84575646 None:intergenic 35.0%
! ATCTAACTGGTAACTGGTTT+AGG + chr4_1:84575831-84575850 Msa0579720:intron 35.0%
ATGTCACAAGCAATTAATCC+TGG + chr4_1:84576011-84576030 Msa0579720:intron 35.0%
AATTCATGCAACTATGATCC+AGG - chr4_1:84576032-84576051 None:intergenic 35.0%
TAGAATTGGGAAAGAATGAG+TGG - chr4_1:84576169-84576188 None:intergenic 35.0%
CATTCTTTCCCAATTCTAGA+TGG + chr4_1:84576171-84576190 Msa0579720:intron 35.0%
TTCTTTCCCAATTCTAGATG+GGG + chr4_1:84576173-84576192 Msa0579720:intron 35.0%
!! TTTCAAGTTAACCTTACAGC+AGG + chr4_1:84576533-84576552 Msa0579720:CDS 35.0%
CAAACATGAAGAATGGAATG+AGG - chr4_1:84576605-84576624 None:intergenic 35.0%
AAATATCCTCAGTCCACAAA+AGG - chr4_1:84576656-84576675 None:intergenic 35.0%
TCTCTTTCTCTTAAACTTGC+TGG - chr4_1:84576897-84576916 None:intergenic 35.0%
AGCAATATAGAGACTGTAGA+TGG + chr4_1:84576930-84576949 Msa0579720:three_prime_UTR 35.0%
AGAGTATGGAATGAATGTAG+AGG + chr4_1:84576961-84576980 Msa0579720:three_prime_UTR 35.0%
TAAAAAAAGGTCTAGCTGAC+TGG - chr4_1:84577015-84577034 None:intergenic 35.0%
!!! TTTATTTTTGTGGCCACCTA+TGG - chr4_1:84577057-84577076 None:intergenic 35.0%
!! GACATTGATTTGTCACACAA+AGG - chr4_1:84577091-84577110 None:intergenic 35.0%
TCCTTATCTTACTTGCAACA+GGG - chr4_1:84577188-84577207 None:intergenic 35.0%
TTCCTTATCTTACTTGCAAC+AGG - chr4_1:84577189-84577208 None:intergenic 35.0%
GAATTGAGAACAACATGTGT+TGG + chr4_1:84577311-84577330 Msa0579720:three_prime_UTR 35.0%
TTCAAAGGCAACACAATGAA+GGG - chr4_1:84577418-84577437 None:intergenic 35.0%
TTCATTGTGTTGCCTTTGAA+GGG + chr4_1:84577418-84577437 Msa0579720:three_prime_UTR 35.0%
CAAGTTTCTGTTAGCAATGT+GGG - chr4_1:84577455-84577474 None:intergenic 35.0%
TCAAGTTTCTGTTAGCAATG+TGG - chr4_1:84577456-84577475 None:intergenic 35.0%
ACAAACTGGAAACTGTAGAT+GGG - chr4_1:84577581-84577600 None:intergenic 35.0%
AACAAACTGGAAACTGTAGA+TGG - chr4_1:84577582-84577601 None:intergenic 35.0%
ACAAACTGGAAACTGTAGAT+GGG - chr4_1:84577581-84577600 None:intergenic 35.0%
AACAAACTGGAAACTGTAGA+TGG - chr4_1:84577582-84577601 None:intergenic 35.0%
!! CATGGACAGGTTTGTCATTT+TGG + chr4_1:84575709-84575728 Msa0579720:intron 40.0%
TCAGGAAATTGTATGCACCA+TGG - chr4_1:84575770-84575789 None:intergenic 40.0%
! CATCAACCCCATCTAGAATT+GGG - chr4_1:84576182-84576201 None:intergenic 40.0%
GAAATCAACTATATGGCGTC+AGG - chr4_1:84576426-84576445 None:intergenic 40.0%
AATTTCTCTCACCTGCTGTA+AGG - chr4_1:84576547-84576566 None:intergenic 40.0%
TGGTAGACGATGTACAGATA+AGG - chr4_1:84576579-84576598 None:intergenic 40.0%
TATCTGTACATCGTCTACCA+AGG + chr4_1:84576579-84576598 Msa0579720:intron 40.0%
GTGATCTGCAGAAGAATACA+AGG - chr4_1:84576686-84576705 None:intergenic 40.0%
TGCAGATCACATTCTTTCAC+TGG + chr4_1:84576696-84576715 Msa0579720:CDS 40.0%
GATCACATTCTTTCACTGGA+CGG + chr4_1:84576700-84576719 Msa0579720:CDS 40.0%
!! TTCTCTTAAACTTGCTGGAG+AGG - chr4_1:84576892-84576911 None:intergenic 40.0%
AGATGGAGATGTAGAGAGTA+TGG + chr4_1:84576947-84576966 Msa0579720:three_prime_UTR 40.0%
TATTTAACCTACGAAGGGGT+TGG - chr4_1:84576989-84577008 None:intergenic 40.0%
GCTCTATTTAACCTACGAAG+GGG - chr4_1:84576993-84577012 None:intergenic 40.0%
GGCTCTATTTAACCTACGAA+GGG - chr4_1:84576994-84577013 None:intergenic 40.0%
! TGGCTCTATTTAACCTACGA+AGG - chr4_1:84576995-84577014 None:intergenic 40.0%
CTTACTGTGTTCTGTACCAT+AGG + chr4_1:84577038-84577057 Msa0579720:three_prime_UTR 40.0%
ACCCTGTTGCAAGTAAGATA+AGG + chr4_1:84577184-84577203 Msa0579720:three_prime_UTR 40.0%
TGGAAATTCTGCCTTCATTG+TGG + chr4_1:84577331-84577350 Msa0579720:three_prime_UTR 40.0%
CAACACAATGAAGGGTTTGA+AGG - chr4_1:84577410-84577429 None:intergenic 40.0%
CTTCAAAGGCAACACAATGA+AGG - chr4_1:84577419-84577438 None:intergenic 40.0%
CTTCATTGTGTTGCCTTTGA+AGG + chr4_1:84577417-84577436 Msa0579720:three_prime_UTR 40.0%
! TCATTGTGTTGCCTTTGAAG+GGG + chr4_1:84577419-84577438 Msa0579720:three_prime_UTR 40.0%
CTGACTCATCCTTACAAAAC+CGG - chr4_1:84577500-84577519 None:intergenic 40.0%
CAAACTGGAAACTGTAGATG+GGG - chr4_1:84577580-84577599 None:intergenic 40.0%
CAAACTGGAAACTGTAGATG+GGG - chr4_1:84577580-84577599 None:intergenic 40.0%
CAGCTGCATCTTTAACAAAC+TGG - chr4_1:84577706-84577725 None:intergenic 40.0%
!! CGCTGATCGCTAGTTTGTTT+AGG - chr4_1:84575124-84575143 None:intergenic 45.0%
GGCAAAATTGACACCTGATG+AGG + chr4_1:84575611-84575630 Msa0579720:intron 45.0%
!! CGACGCAATAGTTGTTTTGC+CGG + chr4_1:84575866-84575885 Msa0579720:intron 45.0%
! GCATCAACCCCATCTAGAAT+TGG - chr4_1:84576183-84576202 None:intergenic 45.0%
AAGTCGCAAGTTCGCAACAA+AGG - chr4_1:84576453-84576472 None:intergenic 45.0%
TGTTCCTTTACGGCAGTAAC+TGG + chr4_1:84576494-84576513 Msa0579720:intron 45.0%
GTTCCTTTACGGCAGTAACT+GGG + chr4_1:84576495-84576514 Msa0579720:intron 45.0%
TGAAGAATGGAATGAGGCCT+TGG - chr4_1:84576599-84576618 None:intergenic 45.0%
ACTGTGTTCTGTACCATAGG+TGG + chr4_1:84577041-84577060 Msa0579720:three_prime_UTR 45.0%
GACTAAATCCACCCCTTCAA+AGG - chr4_1:84577433-84577452 None:intergenic 45.0%
!! CCTTACAAAACCGGCTTGTA+AGG - chr4_1:84577491-84577510 None:intergenic 45.0%
!! CCTTACAAGCCGGTTTTGTA+AGG + chr4_1:84577488-84577507 Msa0579720:three_prime_UTR 45.0%
!! CGGTTTTGTAAGGATGAGTC+AGG + chr4_1:84577498-84577517 Msa0579720:three_prime_UTR 45.0%
AAACTAGCGATCAGCGAAGC+AGG + chr4_1:84575128-84575147 Msa0579720:five_prime_UTR 50.0%
TCTCCCAGTTACTGCCGTAA+AGG - chr4_1:84576501-84576520 None:intergenic 50.0%
GGACGGCAGTACATTACAGA+AGG + chr4_1:84576717-84576736 Msa0579720:CDS 50.0%
AGGAAACCTTAGGACGCACT+TGG + chr4_1:84577204-84577223 Msa0579720:three_prime_UTR 50.0%
ACTCTACCAAGTGCGTCCTA+AGG - chr4_1:84577213-84577232 None:intergenic 50.0%
ATGCCGCGATTAAGCGAGTA+GGG - chr4_1:84577362-84577381 None:intergenic 50.0%
AATGCCGCGATTAAGCGAGT+AGG - chr4_1:84577363-84577382 None:intergenic 50.0%
GGGTTTGAAGGCTGCAATGA+AGG - chr4_1:84577398-84577417 None:intergenic 50.0%
! TTGTGTTGCCTTTGAAGGGG+TGG + chr4_1:84577422-84577441 Msa0579720:three_prime_UTR 50.0%
!! AACCGGCTTGTAAGGTGAGA+GGG - chr4_1:84577483-84577502 None:intergenic 50.0%
!! AAACCGGCTTGTAAGGTGAG+AGG - chr4_1:84577484-84577503 None:intergenic 50.0%
AGGCAGGCAGGCAACGTAAT+GGG + chr4_1:84575152-84575171 Msa0579720:exon 55.0%
!! TTGTTTCGGGTGCTGCTGCA+TGG + chr4_1:84575691-84575710 Msa0579720:CDS 55.0%
ACGGCAGTAACTGGGAGACT+TGG + chr4_1:84576503-84576522 Msa0579720:intron 55.0%
!! CTGCTGCCTTTTGTGGACTG+AGG + chr4_1:84576647-84576666 Msa0579720:CDS 55.0%
AGAGGATCCAACCCCTTCGT+AGG + chr4_1:84576979-84576998 Msa0579720:three_prime_UTR 55.0%
TGCCGCGATTAAGCGAGTAG+GGG - chr4_1:84577361-84577380 None:intergenic 55.0%
AGCCCTCTCACCTTACAAGC+CGG + chr4_1:84577478-84577497 Msa0579720:three_prime_UTR 55.0%
TAGCGATCAGCGAAGCAGGC+AGG + chr4_1:84575132-84575151 Msa0579720:five_prime_UTR 60.0%
CAGGCAGGCAGGCAACGTAA+TGG + chr4_1:84575151-84575170 Msa0579720:exon 60.0%
! GCAGGAGCTGCTGCCTTTTG+TGG + chr4_1:84576640-84576659 Msa0579720:intron 60.0%
GTAGGGGCGGACCACAATGA+AGG - chr4_1:84577345-84577364 None:intergenic 60.0%
CGCGATTAAGCGAGTAGGGG+CGG - chr4_1:84577358-84577377 None:intergenic 60.0%
CGCCCCTACTCGCTTAATCG+CGG + chr4_1:84577356-84577375 Msa0579720:three_prime_UTR 60.0%
GATCAGCGAAGCAGGCAGGC+AGG + chr4_1:84575136-84575155 Msa0579720:five_prime_UTR 65.0%
!! TCGGGTGCTGCTGCATGGAC+AGG + chr4_1:84575696-84575715 Msa0579720:CDS 65.0%
AGCGAAGCAGGCAGGCAGGC+AGG + chr4_1:84575140-84575159 Msa0579720:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 84575072 84577728 84575072 ID=Msa0579720;Name=Msa0579720
chr4_1 mRNA 84575072 84577728 84575072 ID=Msa0579720-mRNA-1;Parent=Msa0579720;Name=Msa0579720-mRNA-1;_AED=0.23;_eAED=0.23;_QI=98|1|1|1|1|1|4|918|121
chr4_1 exon 84575072 84575217 84575072 ID=Msa0579720-mRNA-1:exon:30904;Parent=Msa0579720-mRNA-1
chr4_1 exon 84575612 84575717 84575612 ID=Msa0579720-mRNA-1:exon:30905;Parent=Msa0579720-mRNA-1
chr4_1 exon 84576510 84576554 84576510 ID=Msa0579720-mRNA-1:exon:30906;Parent=Msa0579720-mRNA-1
chr4_1 exon 84576644 84577728 84576644 ID=Msa0579720-mRNA-1:exon:30907;Parent=Msa0579720-mRNA-1
chr4_1 five_prime_UTR 84575072 84575169 84575072 ID=Msa0579720-mRNA-1:five_prime_utr;Parent=Msa0579720-mRNA-1
chr4_1 CDS 84575170 84575217 84575170 ID=Msa0579720-mRNA-1:cds;Parent=Msa0579720-mRNA-1
chr4_1 CDS 84575612 84575717 84575612 ID=Msa0579720-mRNA-1:cds;Parent=Msa0579720-mRNA-1
chr4_1 CDS 84576510 84576554 84576510 ID=Msa0579720-mRNA-1:cds;Parent=Msa0579720-mRNA-1
chr4_1 CDS 84576644 84576810 84576644 ID=Msa0579720-mRNA-1:cds;Parent=Msa0579720-mRNA-1
chr4_1 three_prime_UTR 84576811 84577728 84576811 ID=Msa0579720-mRNA-1:three_prime_utr;Parent=Msa0579720-mRNA-1
Gene Sequence

>Msa0579720

ATGGGCGAAGCATTGTTCGATCTTGAACAACTTCTCATTTCCAAAAGGGCAAAATTGACACCTGATGAGGTAAACATTCTTCAATCATGCAAAACTAATGCTATAAAGAATTTCACTCTTTTTTCACTTGTTTCGGGTGCTGCTGCATGGACAGTAACTGGGAGACTTGGTAAAGCATTTCAAGTTAACCTTACAGCAGGAGCTGCTGCCTTTTGTGGACTGAGGATATTTAGTAGATCCTTGTATTCTTCTGCAGATCACATTCTTTCACTGGACGGCAGTACATTACAGAAGGAGTTAGCAAATATGTTTGTCTTCTCATCTCAATTGCATTCACTTGATGAACACTTTTGGTTTGTGCAATAA
Protein sequence

>Msa0579720

MGEALFDLEQLLISKRAKLTPDEVNILQSCKTNAIKNFTLFSLVSGAAAWTVTGRLGKAFQVNLTAGAAAFCGLRIFSRSLYSSADHILSLDGSTLQKELANMFVFSSQLHSLDEHFWFVQ*