AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1466460


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466460 MtrunA17_Chr7g0252401 100.000 182 0 0 1 182 132 313 3.83e-130 373
Msa1466460 MtrunA17_Chr8g0385571 71.978 182 49 1 1 182 152 331 1.80e-91 275
Msa1466460 MtrunA17_Chr3g0131501 65.934 182 60 1 1 182 130 309 1.11e-83 254
Msa1466460 MtrunA17_Chr5g0396801 40.650 123 62 3 71 182 29 151 1.33e-20 87.0
Msa1466460 MtrunA17_Chr8g0347451 39.655 116 63 2 74 182 49 164 2.59e-20 86.7
Msa1466460 MtrunA17_Chr6g0488081 37.273 110 63 2 76 182 19 125 1.83e-14 69.7
Msa1466460 MtrunA17_Chr8g0382201 36.082 97 62 0 70 166 189 285 2.89e-14 69.7
Msa1466460 MtrunA17_Chr3g0135861 39.286 84 48 1 76 156 35 118 3.28e-14 69.3
Msa1466460 MtrunA17_Chr1g0149111 38.889 108 64 1 72 179 135 240 4.18e-14 69.3
Msa1466460 MtrunA17_Chr7g0240341 32.500 120 68 2 76 182 25 144 8.06e-13 65.5
Msa1466460 MtrunA17_Chr3g0137941 35.849 106 66 2 74 179 120 223 8.70e-13 65.5
Msa1466460 MtrunA17_Chr3g0115801 33.696 92 61 0 74 165 114 205 2.65e-12 63.9
Msa1466460 MtrunA17_Chr5g0436041 33.051 118 68 3 76 182 34 151 5.15e-12 63.2
Msa1466460 MtrunA17_Chr2g0296061 31.148 122 67 1 78 182 17 138 5.89e-12 62.8
Msa1466460 MtrunA17_Chr4g0067231 32.258 124 65 2 78 182 16 139 1.23e-11 62.0
Msa1466460 MtrunA17_Chr4g0063561 34.862 109 68 2 72 179 119 225 1.99e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466460 AT5G51050.1 69.780 182 54 1 1 182 148 328 5.16e-88 266
Msa1466460 AT5G61810.1 67.033 182 55 1 1 182 133 309 8.68e-83 253
Msa1466460 AT5G07320.1 69.231 182 51 2 1 182 134 310 3.84e-80 246
Msa1466460 AT5G61810.2 66.082 171 53 1 12 182 1 166 1.48e-77 235
Msa1466460 AT4G01100.1 39.655 116 63 2 74 182 40 155 6.63e-21 88.6
Msa1466460 AT4G01100.2 35.385 130 63 2 74 182 40 169 6.96e-19 83.2
Msa1466460 AT4G01100.3 37.500 104 58 2 86 182 2 105 8.88e-17 76.6
Msa1466460 AT3G55640.2 35.714 126 70 3 68 182 30 155 6.40e-14 68.2
Msa1466460 AT3G55640.3 35.714 126 70 3 68 182 30 155 6.40e-14 68.2
Msa1466460 AT3G55640.1 35.714 126 70 3 68 182 30 155 1.49e-13 67.8
Msa1466460 AT3G55640.4 35.714 126 70 3 68 182 30 155 1.49e-13 67.8
Msa1466460 AT4G26180.2 34.545 110 66 2 76 182 21 127 2.82e-13 66.6
Msa1466460 AT4G26180.1 34.545 110 66 2 76 182 21 127 3.62e-13 66.6
Msa1466460 AT4G32400.1 36.111 108 67 1 72 179 110 215 6.84e-13 66.2
Msa1466460 AT3G53940.2 35.833 120 62 3 76 182 73 190 7.14e-13 65.5
Msa1466460 AT3G53940.1 35.833 120 62 3 76 182 73 190 1.20e-12 65.5
Msa1466460 AT3G21390.2 33.333 120 65 2 78 182 43 162 2.60e-12 64.3
Msa1466460 AT3G21390.1 33.333 120 65 2 78 182 21 140 3.44e-12 63.9
Msa1466460 AT1G78180.1 32.990 97 65 0 70 166 122 218 6.47e-12 63.5
Msa1466460 AT5G64970.3 29.897 97 68 0 70 166 129 225 1.30e-11 62.4
Msa1466460 AT5G64970.1 29.897 97 68 0 70 166 129 225 1.55e-11 62.4

Find 53 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGAACATTTACCATTATT+TGG 0.152309 tig0047191:+574 Msa1466460:CDS
CTGCTGGCTGGTGGTATAGC+TGG 0.297668 tig0047191:+965 Msa1466460:CDS
GGAGAGGAAAAGGCTGATAT+TGG 0.305280 tig0047191:+929 Msa1466460:CDS
TTATGCGAGCACTTGTTGTT+TGG 0.340534 tig0047191:-773 Msa1466460:intergenic
TGCATATCCGCTCCAAATAA+TGG 0.343042 tig0047191:-602 Msa1466460:intergenic
CGGATATGCATGGTCGATAT+CGG 0.355218 tig0047191:+599 Msa1466460:CDS
TGGAAAGAAGGTGGTTTAGT+AGG 0.387807 tig0047191:+800 Msa1466460:CDS
TTTCGTCGAGCGTGTTGATA+AGG 0.392137 tig0047191:+481 Msa1466460:CDS
GGATATGCATGGTCGATATC+GGG 0.394180 tig0047191:+600 Msa1466460:CDS
CAATGGCTTAAATGTTCTTA+AGG 0.402824 tig0047191:+835 Msa1466460:CDS
TAAAATCTAATGGCACTCTC+AGG 0.406499 tig0047191:-879 Msa1466460:intergenic
CTCATAGGTATAAAATCTAA+TGG 0.412822 tig0047191:-889 Msa1466460:intergenic
GCATATAGGTATCTTATTGC+AGG 0.428990 tig0047191:+665 Msa1466460:CDS
GAAGGTCTTCATCATCTCAT+AGG 0.451692 tig0047191:-904 Msa1466460:intergenic
TAATGGTGTTATAACATTTG+AGG 0.460039 tig0047191:+508 Msa1466460:CDS
CTTATTGCAGGAGGAGTGGC+AGG 0.466803 tig0047191:+677 Msa1466460:CDS
AAAGGCTGATATTGGAGGTA+TGG 0.476181 tig0047191:+937 Msa1466460:CDS
TGCACCTCTCGACCGTCTAA+AGG 0.491875 tig0047191:+721 Msa1466460:CDS
TGCTAAAGGAGGAGAGGAAA+AGG 0.498282 tig0047191:+919 Msa1466460:CDS
AAGGCTGATATTGGAGGTAT+GGG 0.501323 tig0047191:+938 Msa1466460:CDS
GAGGAAAAGGCTGATATTGG+AGG 0.503465 tig0047191:+932 Msa1466460:CDS
GTGTTATAACATTTGAGGAA+TGG 0.514699 tig0047191:+513 Msa1466460:CDS
AGGGATTAAAATTGATGATG+AGG 0.515030 tig0047191:+448 None:intergenic
ACCTTCATTGTGGATGCTAA+AGG 0.516847 tig0047191:+905 Msa1466460:CDS
TCCTTTAGCATCCACAATGA+AGG 0.516899 tig0047191:-922 Msa1466460:intergenic
TTATAACATTTGAGGAATGG+AGG 0.534313 tig0047191:+516 Msa1466460:CDS
AAAATCTAATGGCACTCTCA+GGG 0.534392 tig0047191:-878 Msa1466460:intergenic
ATGGGGCGGCTGCTGGCTGG+TGG 0.539699 tig0047191:+956 Msa1466460:CDS
CGTGTTGATAAGGATCATAA+TGG 0.546342 tig0047191:+491 Msa1466460:CDS
ACTGTTATTCCCGCAGGTGT+TGG 0.556455 tig0047191:+629 Msa1466460:CDS
ACATGCTTACCAACACCTGC+GGG 0.566513 tig0047191:-654 Msa1466460:intergenic
TATAACATTTGAGGAATGGA+GGG 0.568763 tig0047191:+517 Msa1466460:CDS
AAAACTCTTTGGAAAGAAGG+TGG 0.573548 tig0047191:+791 Msa1466460:CDS
TCTGCTGCTTTATCCTCATG+AGG 0.578499 tig0047191:+544 Msa1466460:CDS
GTAAGCATGTTCATGCATAT+AGG 0.586205 tig0047191:+651 Msa1466460:CDS
GATATGCATGGTCGATATCG+GGG 0.597937 tig0047191:+601 Msa1466460:CDS
TTATTTGGAGCGGATATGCA+TGG 0.601766 tig0047191:+589 Msa1466460:CDS
TTCTCAATAGTTGCCTCATG+AGG 0.605430 tig0047191:-573 Msa1466460:intergenic
GAGTGGCAGGTGCTGCATCA+CGG 0.607001 tig0047191:+690 Msa1466460:CDS
GTATCTTATTGCAGGAGGAG+TGG 0.608780 tig0047191:+673 Msa1466460:CDS
TGGCTTAAATGTTCTTAAGG+TGG 0.612520 tig0047191:+838 Msa1466460:CDS
GAACATACTGTTATTCCCGC+AGG 0.623642 tig0047191:+623 Msa1466460:CDS
CTTGCAATACAACCTTTAGA+CGG 0.625650 tig0047191:-749 Msa1466460:intergenic
ACATTTACCATTATTTGGAG+CGG 0.628260 tig0047191:+579 Msa1466460:CDS
AACATGCTTACCAACACCTG+CGG 0.635492 tig0047191:-655 Msa1466460:intergenic
TGTGGATGCTAAAGGAGGAG+AGG 0.642716 tig0047191:+913 Msa1466460:CDS
TTCATTGTGGATGCTAAAGG+AGG 0.644411 tig0047191:+908 Msa1466460:CDS
TATAGGTATCTTATTGCAGG+AGG 0.651279 tig0047191:+668 Msa1466460:CDS
GATGATGAAGACCTTCATTG+TGG 0.683051 tig0047191:+895 Msa1466460:CDS
ACAACCTTTAGACGGTCGAG+AGG 0.683942 tig0047191:-741 Msa1466460:intergenic
CTGATATTGGAGGTATGGGG+CGG 0.684136 tig0047191:+942 Msa1466460:CDS
AAATCTAATGGCACTCTCAG+GGG 0.691447 tig0047191:-877 Msa1466460:intergenic
AGGCTGATATTGGAGGTATG+GGG 0.704416 tig0047191:+939 Msa1466460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGGTGTTATAACATTTG+AGG + tig0047191:508-527 Msa1466460:CDS 25.0%
! TGAGAACATTTACCATTATT+TGG + tig0047191:574-593 Msa1466460:CDS 25.0%
! CTCATAGGTATAAAATCTAA+TGG - tig0047191:876-895 Msa1466460:intergenic 25.0%
GTGTTATAACATTTGAGGAA+TGG + tig0047191:513-532 Msa1466460:CDS 30.0%
TTATAACATTTGAGGAATGG+AGG + tig0047191:516-535 Msa1466460:CDS 30.0%
TATAACATTTGAGGAATGGA+GGG + tig0047191:517-536 Msa1466460:CDS 30.0%
ACATTTACCATTATTTGGAG+CGG + tig0047191:579-598 Msa1466460:CDS 30.0%
! GTAAAAACTCTTTGGAAAGA+AGG + tig0047191:788-807 Msa1466460:CDS 30.0%
CAATGGCTTAAATGTTCTTA+AGG + tig0047191:835-854 Msa1466460:CDS 30.0%
! CGTGTTGATAAGGATCATAA+TGG + tig0047191:491-510 Msa1466460:CDS 35.0%
GTAAGCATGTTCATGCATAT+AGG + tig0047191:651-670 Msa1466460:CDS 35.0%
GCATATAGGTATCTTATTGC+AGG + tig0047191:665-684 Msa1466460:CDS 35.0%
TATAGGTATCTTATTGCAGG+AGG + tig0047191:668-687 Msa1466460:CDS 35.0%
CTTGCAATACAACCTTTAGA+CGG - tig0047191:736-755 Msa1466460:intergenic 35.0%
! TTCCAAAGAGTTTTTACTGC+AGG - tig0047191:785-804 Msa1466460:intergenic 35.0%
! AAAACTCTTTGGAAAGAAGG+TGG + tig0047191:791-810 Msa1466460:CDS 35.0%
!!! GGTTTAGTAGGATTTTTTCG+AGG + tig0047191:812-831 Msa1466460:CDS 35.0%
TGGCTTAAATGTTCTTAAGG+TGG + tig0047191:838-857 Msa1466460:CDS 35.0%
AAAATCTAATGGCACTCTCA+GGG - tig0047191:865-884 Msa1466460:intergenic 35.0%
TAAAATCTAATGGCACTCTC+AGG - tig0047191:866-885 Msa1466460:intergenic 35.0%
TTCTCAATAGTTGCCTCATG+AGG - tig0047191:560-579 Msa1466460:intergenic 40.0%
TGCATATCCGCTCCAAATAA+TGG - tig0047191:589-608 Msa1466460:intergenic 40.0%
! TTATTTGGAGCGGATATGCA+TGG + tig0047191:589-608 Msa1466460:CDS 40.0%
!! TTATGCGAGCACTTGTTGTT+TGG - tig0047191:760-779 Msa1466460:intergenic 40.0%
TGCCTGCAGTAAAAACTCTT+TGG + tig0047191:780-799 Msa1466460:CDS 40.0%
TGGAAAGAAGGTGGTTTAGT+AGG + tig0047191:800-819 Msa1466460:CDS 40.0%
!!! GTAGGATTTTTTCGAGGCAA+TGG + tig0047191:818-837 Msa1466460:CDS 40.0%
AAATCTAATGGCACTCTCAG+GGG - tig0047191:864-883 Msa1466460:intergenic 40.0%
GAAGGTCTTCATCATCTCAT+AGG - tig0047191:891-910 Msa1466460:intergenic 40.0%
GATGATGAAGACCTTCATTG+TGG + tig0047191:895-914 Msa1466460:CDS 40.0%
ACCTTCATTGTGGATGCTAA+AGG + tig0047191:905-924 Msa1466460:CDS 40.0%
TCCTTTAGCATCCACAATGA+AGG - tig0047191:909-928 Msa1466460:intergenic 40.0%
TTCATTGTGGATGCTAAAGG+AGG + tig0047191:908-927 Msa1466460:CDS 40.0%
AAAGGCTGATATTGGAGGTA+TGG + tig0047191:937-956 Msa1466460:CDS 40.0%
! AAGGCTGATATTGGAGGTAT+GGG + tig0047191:938-957 Msa1466460:CDS 40.0%
! TTTCGTCGAGCGTGTTGATA+AGG + tig0047191:481-500 Msa1466460:CDS 45.0%
!! TCTGCTGCTTTATCCTCATG+AGG + tig0047191:544-563 Msa1466460:CDS 45.0%
CGGATATGCATGGTCGATAT+CGG + tig0047191:599-618 Msa1466460:CDS 45.0%
GGATATGCATGGTCGATATC+GGG + tig0047191:600-619 Msa1466460:CDS 45.0%
GATATGCATGGTCGATATCG+GGG + tig0047191:601-620 Msa1466460:CDS 45.0%
GAACATACTGTTATTCCCGC+AGG + tig0047191:623-642 Msa1466460:CDS 45.0%
AACATGCTTACCAACACCTG+CGG - tig0047191:642-661 Msa1466460:intergenic 45.0%
GTATCTTATTGCAGGAGGAG+TGG + tig0047191:673-692 Msa1466460:CDS 45.0%
TGCTAAAGGAGGAGAGGAAA+AGG + tig0047191:919-938 Msa1466460:CDS 45.0%
GGAGAGGAAAAGGCTGATAT+TGG + tig0047191:929-948 Msa1466460:CDS 45.0%
GAGGAAAAGGCTGATATTGG+AGG + tig0047191:932-951 Msa1466460:CDS 45.0%
! AGGCTGATATTGGAGGTATG+GGG + tig0047191:939-958 Msa1466460:CDS 45.0%
ACTGTTATTCCCGCAGGTGT+TGG + tig0047191:629-648 Msa1466460:CDS 50.0%
ACATGCTTACCAACACCTGC+GGG - tig0047191:641-660 Msa1466460:intergenic 50.0%
ACAACCTTTAGACGGTCGAG+AGG - tig0047191:728-747 Msa1466460:intergenic 50.0%
TGTGGATGCTAAAGGAGGAG+AGG + tig0047191:913-932 Msa1466460:CDS 50.0%
! CTGATATTGGAGGTATGGGG+CGG + tig0047191:942-961 Msa1466460:CDS 50.0%
CTTATTGCAGGAGGAGTGGC+AGG + tig0047191:677-696 Msa1466460:CDS 55.0%
TGCACCTCTCGACCGTCTAA+AGG + tig0047191:721-740 Msa1466460:CDS 55.0%
! GAGTGGCAGGTGCTGCATCA+CGG + tig0047191:690-709 Msa1466460:CDS 60.0%
! CTGCTGGCTGGTGGTATAGC+TGG + tig0047191:965-984 Msa1466460:CDS 60.0%
! TGGAGGTATGGGGCGGCTGC+TGG + tig0047191:949-968 Msa1466460:CDS 70.0%
GGTATGGGGCGGCTGCTGGC+TGG + tig0047191:953-972 Msa1466460:CDS 75.0%
! ATGGGGCGGCTGCTGGCTGG+TGG + tig0047191:956-975 Msa1466460:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0047191 gene 452 997 452 ID=Msa1466460;Name=Msa1466460
tig0047191 mRNA 452 997 452 ID=Msa1466460-mRNA-1;Parent=Msa1466460;Name=Msa1466460-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|182
tig0047191 exon 452 997 452 ID=Msa1466460-mRNA-1:exon:25076;Parent=Msa1466460-mRNA-1
tig0047191 CDS 452 997 452 ID=Msa1466460-mRNA-1:cds;Parent=Msa1466460-mRNA-1
Gene Sequence

>Msa1466460

ATTAAAATTGATGATGAGGAACTTGCTCATTTCGTCGAGCGTGTTGATAAGGATCATAATGGTGTTATAACATTTGAGGAATGGAGGGATTTTCTGCTGCTTTATCCTCATGAGGCAACTATTGAGAACATTTACCATTATTTGGAGCGGATATGCATGGTCGATATCGGGGAACATACTGTTATTCCCGCAGGTGTTGGTAAGCATGTTCATGCATATAGGTATCTTATTGCAGGAGGAGTGGCAGGTGCTGCATCACGGACAGCAACTGCACCTCTCGACCGTCTAAAGGTTGTATTGCAAGTCCAAACAACAAGTGCTCGCATAATGCCTGCAGTAAAAACTCTTTGGAAAGAAGGTGGTTTAGTAGGATTTTTTCGAGGCAATGGCTTAAATGTTCTTAAGGTGGCCCCTGAGAGTGCCATTAGATTTTATACCTATGAGATGATGAAGACCTTCATTGTGGATGCTAAAGGAGGAGAGGAAAAGGCTGATATTGGAGGTATGGGGCGGCTGCTGGCTGGTGGTATAGCTGGTGCTGTAGCT

Protein sequence

>Msa1466460

IKIDDEELAHFVERVDKDHNGVITFEEWRDFLLLYPHEATIENIYHYLERICMVDIGEHTVIPAGVGKHVHAYRYLIAGGVAGAASRTATAPLDRLKVVLQVQTTSARIMPAVKTLWKEGGLVGFFRGNGLNVLKVAPESAIRFYTYEMMKTFIVDAKGGEEKADIGGMGRLLAGGIAGAVA