AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0962070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0962070 MtrunA17_Chr6g0481911 97.044 203 6 0 1 203 1 203 1.68e-146 405
Msa0962070 MtrunA17_Chr6g0481941 81.553 206 34 2 1 203 1 205 2.61e-117 332
Msa0962070 MtrunA17_Chr6g0482041 42.326 215 105 8 1 201 1 210 1.44e-46 152
Msa0962070 MtrunA17_Chr7g0228581 41.935 186 94 7 23 203 26 202 3.42e-37 128
Msa0962070 MtrunA17_Chr6g0481951 40.933 193 99 7 21 203 26 213 2.83e-35 123
Msa0962070 MtrunA17_Chr6g0481961 40.415 193 100 7 21 203 26 213 5.62e-34 120
Msa0962070 MtrunA17_Chr6g0482031 36.923 195 104 7 21 203 25 212 1.80e-30 111
Msa0962070 MtrunA17_Chr6g0482011 40.000 175 93 7 24 191 25 194 8.15e-30 109
Msa0962070 MtrunA17_Chr3g0137411 35.377 212 113 8 1 202 1 198 1.16e-24 96.3
Msa0962070 MtrunA17_Chr6g0482021 32.961 179 109 6 26 203 31 199 1.50e-23 92.8
Msa0962070 MtrunA17_Chr3g0137421 34.031 191 101 8 21 203 24 197 5.62e-22 89.0
Msa0962070 MtrunA17_Chr2g0314431 31.980 197 120 7 10 203 14 199 6.40e-20 83.6
Msa0962070 MtrunA17_Chr3g0137441 33.333 189 103 9 23 203 26 199 9.83e-20 83.2
Msa0962070 MtrunA17_Chr1g0173431 31.527 203 120 10 8 202 9 200 5.49e-19 80.9
Msa0962070 MtrunA17_Chr2g0314441 32.367 207 116 10 5 203 8 198 8.37e-19 80.5
Msa0962070 MtrunA17_Chr4g0022751 29.412 204 128 9 5 202 8 201 1.93e-17 77.0
Msa0962070 MtrunA17_Chr6g0476921 31.720 186 118 7 20 202 22 201 5.28e-17 75.9
Msa0962070 MtrunA17_Chr6g0476871 34.054 185 115 6 22 203 28 208 5.56e-17 76.3
Msa0962070 MtrunA17_Chr3g0104591 30.233 172 115 4 18 189 21 187 2.75e-16 73.9
Msa0962070 MtrunA17_Chr6g0476831 32.227 211 128 8 1 203 1 204 4.27e-16 73.9
Msa0962070 MtrunA17_Chr6g0476951 31.068 206 128 7 1 203 1 195 6.68e-16 72.8
Msa0962070 MtrunA17_Chr8g0359111 31.553 206 122 12 7 202 5 201 1.20e-15 72.0
Msa0962070 MtrunA17_Chr4g0022761 29.756 205 122 10 5 202 8 197 1.76e-15 71.6
Msa0962070 MtrunA17_Chr3g0082681 30.542 203 124 8 8 202 9 202 1.86e-15 71.6
Msa0962070 MtrunA17_Chr6g0476961 32.039 206 125 9 1 203 1 194 6.23e-15 70.1
Msa0962070 MtrunA17_Chr3g0082671 29.064 203 127 8 8 202 9 202 8.99e-15 69.7
Msa0962070 MtrunA17_Chr6g0477751 29.327 208 131 8 5 203 4 204 5.71e-13 65.1
Msa0962070 MtrunA17_Chr6g0452491 30.435 207 128 9 5 203 4 202 6.76e-13 64.7
Msa0962070 MtrunA17_Chr6g0452521 30.769 208 127 10 5 203 36 235 3.63e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0962070 AT1G17860.1 47.368 209 90 9 1 203 1 195 2.38e-53 169
Msa0962070 AT1G73260.1 36.464 181 107 5 26 203 30 205 1.20e-28 107
Msa0962070 AT1G73325.1 33.645 214 120 8 1 203 1 203 4.85e-23 92.4

Find 38 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTAGGGTTGTGTTTAAA+AGG 0.160681 6_3:-84917218 Msa0962070:CDS
GTGATCAAATTCCATCATTT+AGG 0.169249 6_3:-84917233 Msa0962070:CDS
ACACCTTGTTTAGCATTATA+AGG 0.259304 6_3:+84917546 None:intergenic
GTATGGCAACTGTTGGTAAT+TGG 0.271386 6_3:-84917386 Msa0962070:CDS
GAACCAGTTCTTGACATTTC+AGG 0.287884 6_3:-84917732 Msa0962070:CDS
GCTTCTCTTGAACAACATAA+AGG 0.342977 6_3:+84917604 None:intergenic
TGATCAAATTCCATCATTTA+GGG 0.346172 6_3:-84917232 Msa0962070:CDS
CAAACTTTGTGTAGGGAACT+AGG 0.366708 6_3:-84917297 Msa0962070:CDS
CTTCTCTTGAACAACATAAA+GGG 0.369437 6_3:+84917605 None:intergenic
AGGGTTGTTTGTTGATGATA+AGG 0.370874 6_3:-84917277 Msa0962070:CDS
AATGTCCTCAAACACAAGTT+TGG 0.377880 6_3:-84917473 Msa0962070:CDS
TTACCAACAGTTGCCATACT+TGG 0.386300 6_3:+84917390 None:intergenic
AGCTTCCAAACTTGTGTTTG+AGG 0.408512 6_3:+84917468 None:intergenic
GATGGAAAATGCTAAGACTA+AGG 0.440045 6_3:+84917794 None:intergenic
GGGTTGTTTGTTGATGATAA+GGG 0.441383 6_3:-84917276 Msa0962070:CDS
TTAAACACAACCCTAAATGA+TGG 0.447080 6_3:+84917222 None:intergenic
TGTAGAAGGTAATCCAAGTA+TGG 0.452203 6_3:-84917403 Msa0962070:CDS
ATTTAGGGTTGTGTTTAAAA+GGG 0.479397 6_3:-84917217 Msa0962070:CDS
GAAATGTCAAGAACTGGTTC+AGG 0.479615 6_3:+84917735 None:intergenic
TGACATTTGCAAGCTTCAGC+AGG 0.493041 6_3:+84917318 None:intergenic
AAGCTTCTTAAAGAGTTGAC+AGG 0.493248 6_3:-84917450 Msa0962070:CDS
TTACCAGTCATAAAAGGTAA+AGG 0.497856 6_3:-84917675 Msa0962070:CDS
GCAAATGTCAAACTTTGTGT+AGG 0.501531 6_3:-84917305 Msa0962070:CDS
TTGCCTGAAATGTCAAGAAC+TGG 0.505604 6_3:+84917729 None:intergenic
CAAATGTCAAACTTTGTGTA+GGG 0.511038 6_3:-84917304 Msa0962070:CDS
TCAGGCAAACAAGTAACAAC+GGG 0.511476 6_3:-84917714 Msa0962070:CDS
TTAAAGAGTTGACAGGGGTG+TGG 0.531517 6_3:-84917443 Msa0962070:CDS
GAGAAGCTTGAAGTAAAGAA+TGG 0.531990 6_3:-84917588 Msa0962070:CDS
AAACTTTGTGTAGGGAACTA+GGG 0.532296 6_3:-84917296 Msa0962070:CDS
GTCATAAAAGGTAAAGGTGG+TGG 0.532373 6_3:-84917669 Msa0962070:CDS
AGCTTCTTAAAGAGTTGACA+GGG 0.566536 6_3:-84917449 Msa0962070:CDS
TTCTCTTGAACAACATAAAG+GGG 0.573118 6_3:+84917606 None:intergenic
AATCCAAGTATGGCAACTGT+TGG 0.585675 6_3:-84917393 Msa0962070:CDS
TTCAGGCAAACAAGTAACAA+CGG 0.605095 6_3:-84917715 Msa0962070:CDS
CCAGTCATAAAAGGTAAAGG+TGG 0.625936 6_3:-84917672 Msa0962070:CDS
ACACCTTATAATGCTAAACA+AGG 0.651507 6_3:-84917549 Msa0962070:CDS
GGTTTAACAGTTGCAAACCA+TGG 0.676406 6_3:-84917648 Msa0962070:CDS
GCTTCTTAAAGAGTTGACAG+GGG 0.746376 6_3:-84917448 Msa0962070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTTACCAGTCATAAA+AGG - chr6_3:84917332-84917351 Msa0962070:CDS 20.0%
!!! ACATTTAGTTTTTGTTACAA+AGG + chr6_3:84917525-84917544 None:intergenic 20.0%
! TGATCAAATTCCATCATTTA+GGG - chr6_3:84917781-84917800 Msa0962070:CDS 25.0%
!! ATTTAGGGTTGTGTTTAAAA+GGG - chr6_3:84917796-84917815 Msa0962070:CDS 25.0%
AATGAAGGCTAAAAAGATGA+TGG + chr6_3:84917240-84917259 None:intergenic 30.0%
!! TGCAATAGTTTTGCAAATGA+AGG + chr6_3:84917255-84917274 None:intergenic 30.0%
TTACCAGTCATAAAAGGTAA+AGG - chr6_3:84917338-84917357 Msa0962070:CDS 30.0%
! ATGTTTGATTGTTTTCACCA+TGG + chr6_3:84917385-84917404 None:intergenic 30.0%
TTCTCTTGAACAACATAAAG+GGG + chr6_3:84917410-84917429 None:intergenic 30.0%
CTTCTCTTGAACAACATAAA+GGG + chr6_3:84917411-84917430 None:intergenic 30.0%
ACACCTTATAATGCTAAACA+AGG - chr6_3:84917464-84917483 Msa0962070:CDS 30.0%
! ACACCTTGTTTAGCATTATA+AGG + chr6_3:84917470-84917489 None:intergenic 30.0%
CAAATGTCAAACTTTGTGTA+GGG - chr6_3:84917709-84917728 Msa0962070:CDS 30.0%
GTGATCAAATTCCATCATTT+AGG - chr6_3:84917780-84917799 Msa0962070:CDS 30.0%
TTAAACACAACCCTAAATGA+TGG + chr6_3:84917794-84917813 None:intergenic 30.0%
! CATTTAGGGTTGTGTTTAAA+AGG - chr6_3:84917795-84917814 Msa0962070:CDS 30.0%
GATGGAAAATGCTAAGACTA+AGG + chr6_3:84917222-84917241 None:intergenic 35.0%
TTCAGGCAAACAAGTAACAA+CGG - chr6_3:84917298-84917317 Msa0962070:CDS 35.0%
GCTTCTCTTGAACAACATAA+AGG + chr6_3:84917412-84917431 None:intergenic 35.0%
GAGAAGCTTGAAGTAAAGAA+TGG - chr6_3:84917425-84917444 Msa0962070:CDS 35.0%
AATGTCCTCAAACACAAGTT+TGG - chr6_3:84917540-84917559 Msa0962070:CDS 35.0%
AAGCTTCTTAAAGAGTTGAC+AGG - chr6_3:84917563-84917582 Msa0962070:CDS 35.0%
AGCTTCTTAAAGAGTTGACA+GGG - chr6_3:84917564-84917583 Msa0962070:CDS 35.0%
TGTAGAAGGTAATCCAAGTA+TGG - chr6_3:84917610-84917629 Msa0962070:CDS 35.0%
GCAAATGTCAAACTTTGTGT+AGG - chr6_3:84917708-84917727 Msa0962070:CDS 35.0%
AAACTTTGTGTAGGGAACTA+GGG - chr6_3:84917717-84917736 Msa0962070:CDS 35.0%
!! AGGGTTGTTTGTTGATGATA+AGG - chr6_3:84917736-84917755 Msa0962070:CDS 35.0%
!! GGGTTGTTTGTTGATGATAA+GGG - chr6_3:84917737-84917756 Msa0962070:CDS 35.0%
! GAAATGTCAAGAACTGGTTC+AGG + chr6_3:84917281-84917300 None:intergenic 40.0%
GAACCAGTTCTTGACATTTC+AGG - chr6_3:84917281-84917300 Msa0962070:CDS 40.0%
TTGCCTGAAATGTCAAGAAC+TGG + chr6_3:84917287-84917306 None:intergenic 40.0%
TCAGGCAAACAAGTAACAAC+GGG - chr6_3:84917299-84917318 Msa0962070:CDS 40.0%
! CCACCTTTACCTTTTATGAC+TGG + chr6_3:84917344-84917363 None:intergenic 40.0%
CCAGTCATAAAAGGTAAAGG+TGG - chr6_3:84917341-84917360 Msa0962070:CDS 40.0%
GTCATAAAAGGTAAAGGTGG+TGG - chr6_3:84917344-84917363 Msa0962070:CDS 40.0%
GGTTTAACAGTTGCAAACCA+TGG - chr6_3:84917365-84917384 Msa0962070:CDS 40.0%
AGCTTCCAAACTTGTGTTTG+AGG + chr6_3:84917548-84917567 None:intergenic 40.0%
GCTTCTTAAAGAGTTGACAG+GGG - chr6_3:84917565-84917584 Msa0962070:CDS 40.0%
AATCCAAGTATGGCAACTGT+TGG - chr6_3:84917620-84917639 Msa0962070:CDS 40.0%
TTACCAACAGTTGCCATACT+TGG + chr6_3:84917626-84917645 None:intergenic 40.0%
!! GTATGGCAACTGTTGGTAAT+TGG - chr6_3:84917627-84917646 Msa0962070:CDS 40.0%
CAAACTTTGTGTAGGGAACT+AGG - chr6_3:84917716-84917735 Msa0962070:CDS 40.0%
TTAAAGAGTTGACAGGGGTG+TGG - chr6_3:84917570-84917589 Msa0962070:CDS 45.0%
!!! GGGTGTGGTTTTTAGCTACA+GGG - chr6_3:84917585-84917604 Msa0962070:CDS 45.0%
!!! GGTGTGGTTTTTAGCTACAG+GGG - chr6_3:84917586-84917605 Msa0962070:CDS 45.0%
!!! GTGTGGTTTTTAGCTACAGG+GGG - chr6_3:84917587-84917606 Msa0962070:CDS 45.0%
TGACATTTGCAAGCTTCAGC+AGG + chr6_3:84917698-84917717 None:intergenic 45.0%
!!! GGGGTGTGGTTTTTAGCTAC+AGG - chr6_3:84917584-84917603 Msa0962070:CDS 50.0%
! TTAGCTACAGGGGGTGTAGA+AGG - chr6_3:84917596-84917615 Msa0962070:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 84917212 84917823 84917212 ID=Msa0962070;Name=Msa0962070
chr6_3 mRNA 84917212 84917823 84917212 ID=Msa0962070-mRNA-1;Parent=Msa0962070;Name=Msa0962070-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|203
chr6_3 exon 84917212 84917823 84917212 ID=Msa0962070-mRNA-1:exon:15235;Parent=Msa0962070-mRNA-1
chr6_3 CDS 84917212 84917823 84917212 ID=Msa0962070-mRNA-1:cds;Parent=Msa0962070-mRNA-1
Gene Sequence

>Msa0962070

ATGAAAACCTTAGTCTTAGCATTTTCCATCATCTTTTTAGCCTTCATTTGCAAAACTATTGCAGCACCTGAACCAGTTCTTGACATTTCAGGCAAACAAGTAACAACGGGTGTGAAATACTATATTTTACCAGTCATAAAAGGTAAAGGTGGTGGTTTAACAGTTGCAAACCATGGTGAAAACAATCAAACATGTCCCCTTTATGTTGTTCAAGAGAAGCTTGAAGTAAAGAATGGTGAAGCAGTTACTTTCACACCTTATAATGCTAAACAAGGTGTGATTCTAACTTCAACTGATCTCAATATTAAGTCCTTTGTAACAAAAACTAAATGTCCTCAAACACAAGTTTGGAAGCTTCTTAAAGAGTTGACAGGGGTGTGGTTTTTAGCTACAGGGGGTGTAGAAGGTAATCCAAGTATGGCAACTGTTGGTAATTGGTTTAAGATTGAGAAAGCTGATAAAGATTATGTGCTTTCTTTCTGTCCTGCTGAAGCTTGCAAATGTCAAACTTTGTGTAGGGAACTAGGGTTGTTTGTTGATGATAAGGGAAATAAGCACTTAGCTCTTAGTGATCAAATTCCATCATTTAGGGTTGTGTTTAAAAGGGCTTGA

Protein sequence

>Msa0962070

MKTLVLAFSIIFLAFICKTIAAPEPVLDISGKQVTTGVKYYILPVIKGKGGGLTVANHGENNQTCPLYVVQEKLEVKNGEAVTFTPYNAKQGVILTSTDLNIKSFVTKTKCPQTQVWKLLKELTGVWFLATGGVEGNPSMATVGNWFKIEKADKDYVLSFCPAEACKCQTLCRELGLFVDDKGNKHLALSDQIPSFRVVFKRA*