AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0198800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0198800 MtrunA17_Chr2g0293221 90.533 169 15 1 1 169 212 379 2.04e-101 296
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0198800 AT1G19980.1 38.824 170 85 7 1 166 182 336 1.06e-21 90.1

Find 67 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGTGCAACGATTTAAAA+AGG 0.168184 2_1:-17486550 None:intergenic
GTGGAATCAATTTAATAAAA+TGG 0.179907 2_1:+17486278 Msa0198800:exon
GTCTCAGTAGTGGGAATAAT+TGG 0.182246 2_1:-17487404 None:intergenic
CAAAACTAAAGTCATCTTTA+AGG 0.195780 2_1:+17487459 Msa0198800:CDS
TAGTGACTCTTTCTTTGTTC+AGG 0.219957 2_1:-17486626 None:intergenic
TAACTTGTTAGTAGATATTA+TGG 0.232979 2_1:+17487516 Msa0198800:three_prime_UTR
CTTAAAGATGACTTTAGTTT+TGG 0.240955 2_1:-17487458 None:intergenic
TACCCTGCTCAGCTCATCTT+TGG 0.262415 2_1:-17486181 None:intergenic
ACCTCAGCAAATAGTTTAAA+AGG 0.262684 2_1:-17485838 None:intergenic
TCTCAATATCACTTTCAAAT+TGG 0.267779 2_1:+17485750 Msa0198800:five_prime_UTR
CAAACTACTCTCTAGCTAAA+AGG 0.299294 2_1:-17487801 None:intergenic
ATTGTTATAATTAGCTTTGT+TGG 0.312386 2_1:+17485864 Msa0198800:five_prime_UTR
AGTGACTCTTTCTTTGTTCA+GGG 0.333096 2_1:-17486625 None:intergenic
AGAAAGTAAAGTGGCTGATA+TGG 0.336902 2_1:+17486437 Msa0198800:CDS
GCAGTCTGAAAATATTAAAA+AGG 0.342062 2_1:+17486395 Msa0198800:CDS
ACTGTTCTCTTCCAACTTCA+AGG 0.343289 2_1:+17487433 Msa0198800:CDS
AGCTCATCTTTGGTTTCATC+AGG 0.351979 2_1:-17486171 None:intergenic
ACTGTGTGTGTTTGGAAATA+TGG 0.356173 2_1:-17487635 None:intergenic
GTGAGCTTTCTGTTTGGAAT+AGG 0.362590 2_1:+17487710 Msa0198800:three_prime_UTR
AGTAGATATTATGGTGTGTT+TGG 0.372558 2_1:+17487525 Msa0198800:three_prime_UTR
ATCACTTTCAAATTGGCATT+GGG 0.407727 2_1:+17485757 Msa0198800:five_prime_UTR
AAGTTGGAAGAGAACAGTTT+GGG 0.411264 2_1:-17487428 None:intergenic
AGAGAGTACAAAGGATGATT+TGG 0.418152 2_1:+17485914 Msa0198800:five_prime_UTR
ACATCAAATAACAGTGATAC+TGG 0.422056 2_1:+17486567 Msa0198800:CDS
AGATTAAACAAGGAAATATC+AGG 0.427351 2_1:+17486474 Msa0198800:CDS
AATACAAGACTGTGTGTGTT+TGG 0.428323 2_1:-17487643 None:intergenic
GAAGTTGGAAGAGAACAGTT+TGG 0.431216 2_1:-17487429 None:intergenic
CTCTTGCCAGTATCCTTCAA+TGG 0.432868 2_1:-17486081 None:intergenic
AAACCAAAGATGAGCTGAGC+AGG 0.433798 2_1:+17486178 Msa0198800:five_prime_UTR
CTCCACAAATTTCTCAGAAA+AGG 0.435679 2_1:+17486674 Msa0198800:CDS
TATCACTTTCAAATTGGCAT+TGG 0.437559 2_1:+17485756 Msa0198800:five_prime_UTR
CTGGAAAAGTTGAGAGTAGC+AGG 0.440032 2_1:+17486586 Msa0198800:CDS
TAAACAAGAAGAAGTAGAAA+AGG 0.440876 2_1:+17486329 Msa0198800:CDS
GGAACGCCATTGAAGGATAC+TGG 0.442386 2_1:+17486075 Msa0198800:five_prime_UTR
TATCCTTCAATGGCGTTCCT+TGG 0.442878 2_1:-17486071 None:intergenic
TTCTGAGAAATTTGTGGAGG+TGG 0.449334 2_1:-17486670 None:intergenic
AAAACATACTTCTCCATTCG+AGG 0.451403 2_1:-17485976 None:intergenic
TGATTAGTGAGCTTTCTGTT+TGG 0.467706 2_1:+17487704 Msa0198800:three_prime_UTR
GAGGTGGAAGCATGTTCATC+AGG 0.475857 2_1:-17486654 None:intergenic
TATGGTGTGTTTGGAACCAT+CGG 0.502703 2_1:+17487534 Msa0198800:three_prime_UTR
TCTCAGTAGTGGGAATAATT+GGG 0.507090 2_1:-17487403 None:intergenic
CTTCTTCTTCCCACTACTTG+AGG 0.508521 2_1:-17486115 None:intergenic
AAGAAAGGAACCTCAAGTAG+TGG 0.509613 2_1:+17486105 Msa0198800:five_prime_UTR
GAGTACAAAGGATGATTTGG+AGG 0.515610 2_1:+17485917 Msa0198800:five_prime_UTR
GGACCAAGGAACGCCATTGA+AGG 0.522380 2_1:+17486068 Msa0198800:intron
AAAAGAAGCTTGAAGAGGAA+AGG 0.524428 2_1:+17487377 Msa0198800:CDS
TACATAATTCAGCTCAAAAG+TGG 0.525721 2_1:-17487769 None:intergenic
AAACATACTTGTTTCTAGCA+TGG 0.526753 2_1:+17486365 Msa0198800:CDS
TCTTGTATTTGTTTATTGCA+TGG 0.529309 2_1:+17487657 Msa0198800:three_prime_UTR
GATACTGGCAAGAGGAAGAA+AGG 0.541904 2_1:+17486090 Msa0198800:five_prime_UTR
TTGAGAAAGGTAATCAAACC+CGG 0.569015 2_1:-17485949 None:intergenic
ATCTGAGTTAGAAAGTAAAG+TGG 0.569525 2_1:+17486428 Msa0198800:CDS
GACTCTTTCTTTGTTCAGGG+TGG 0.570173 2_1:-17486622 None:intergenic
AGAAAGGAACCTCAAGTAGT+GGG 0.601571 2_1:+17486106 Msa0198800:five_prime_UTR
TAAAGTGGCTGATATGGATG+CGG 0.611402 2_1:+17486443 Msa0198800:CDS
AGTTTGGGAGTCTCAGTAGT+GGG 0.624179 2_1:-17487413 None:intergenic
AGGAGAAAAGAAGCTTGAAG+AGG 0.631901 2_1:+17487372 Msa0198800:intron
GGCCACACCGTGAAAACCGA+TGG 0.632545 2_1:-17487550 None:intergenic
AACCAAAGATGAGCTGAGCA+GGG 0.640278 2_1:+17486179 Msa0198800:five_prime_UTR
CAGTTTGGGAGTCTCAGTAG+TGG 0.642972 2_1:-17487414 None:intergenic
CATACTTGTTTCTAGCATGG+AGG 0.643179 2_1:+17486368 Msa0198800:CDS
GGAAATATCAGGACTCTCAG+CGG 0.661029 2_1:+17486485 Msa0198800:CDS
TTTGACTGCAGAGAGTACAA+AGG 0.671398 2_1:+17485905 Msa0198800:five_prime_UTR
CATTGAAGGATACTGGCAAG+AGG 0.671920 2_1:+17486082 Msa0198800:five_prime_UTR
GTCACTTTATAACAGGACCA+AGG 0.685155 2_1:+17486054 Msa0198800:intron
CGAAATTAGTAAATTCAACG+TGG 0.703351 2_1:+17485783 Msa0198800:five_prime_UTR
GGTAAAGAGAGCTTAACGTG+CGG 0.756119 2_1:-17487614 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACACTAATACTTTGTT+TGG - chr2_1:17485811-17485830 None:intergenic 20.0%
!!! ATTGTTATAATTAGCTTTGT+TGG + chr2_1:17485864-17485883 Msa0198800:five_prime_UTR 20.0%
!!! TATGTTTTCTGATATTTATC+AGG + chr2_1:17485991-17486010 Msa0198800:intron 20.0%
!! GATAGTAATGACTAATAAAA+AGG - chr2_1:17486017-17486036 None:intergenic 20.0%
!! GTGGAATCAATTTAATAAAA+TGG + chr2_1:17486278-17486297 Msa0198800:exon 20.0%
!! ATCATTTAATACATACAGTA+CGG - chr2_1:17486737-17486756 None:intergenic 20.0%
!! TATTAAATGATTCTCTTCTA+TGG + chr2_1:17486746-17486765 Msa0198800:intron 20.0%
!! CAAATAAAATCATAAGAAAC+AGG - chr2_1:17487322-17487341 None:intergenic 20.0%
!!! TAACTTGTTAGTAGATATTA+TGG + chr2_1:17487516-17487535 Msa0198800:three_prime_UTR 20.0%
!! AAAAACACTAATACTTTGTT+TGG - chr2_1:17485811-17485830 None:intergenic 20.0%
!!! ATTGTTATAATTAGCTTTGT+TGG + chr2_1:17485864-17485883 Msa0198800:five_prime_UTR 20.0%
!!! TATGTTTTCTGATATTTATC+AGG + chr2_1:17485991-17486010 Msa0198800:intron 20.0%
!! GATAGTAATGACTAATAAAA+AGG - chr2_1:17486017-17486036 None:intergenic 20.0%
!! GTGGAATCAATTTAATAAAA+TGG + chr2_1:17486278-17486297 Msa0198800:exon 20.0%
!! ATCATTTAATACATACAGTA+CGG - chr2_1:17486737-17486756 None:intergenic 20.0%
!! TATTAAATGATTCTCTTCTA+TGG + chr2_1:17486746-17486765 Msa0198800:intron 20.0%
!! CAAATAAAATCATAAGAAAC+AGG - chr2_1:17487322-17487341 None:intergenic 20.0%
!!! TAACTTGTTAGTAGATATTA+TGG + chr2_1:17487516-17487535 Msa0198800:three_prime_UTR 20.0%
! TCTCAATATCACTTTCAAAT+TGG + chr2_1:17485750-17485769 Msa0198800:five_prime_UTR 25.0%
! TAAACAAGAAGAAGTAGAAA+AGG + chr2_1:17486329-17486348 Msa0198800:CDS 25.0%
! GCAGTCTGAAAATATTAAAA+AGG + chr2_1:17486395-17486414 Msa0198800:CDS 25.0%
! GGAAACAAAAAGATTAAACA+AGG + chr2_1:17486464-17486483 Msa0198800:CDS 25.0%
! AGATTAAACAAGGAAATATC+AGG + chr2_1:17486474-17486493 Msa0198800:CDS 25.0%
! ATCTTGGTGAATATATTCAT+AGG - chr2_1:17487263-17487282 None:intergenic 25.0%
!!! CTTAAAGATGACTTTAGTTT+TGG - chr2_1:17487461-17487480 None:intergenic 25.0%
! CAAAACTAAAGTCATCTTTA+AGG + chr2_1:17487459-17487478 Msa0198800:CDS 25.0%
! TCTTGTATTTGTTTATTGCA+TGG + chr2_1:17487657-17487676 Msa0198800:three_prime_UTR 25.0%
! TCTCAATATCACTTTCAAAT+TGG + chr2_1:17485750-17485769 Msa0198800:five_prime_UTR 25.0%
! TAAACAAGAAGAAGTAGAAA+AGG + chr2_1:17486329-17486348 Msa0198800:CDS 25.0%
! GCAGTCTGAAAATATTAAAA+AGG + chr2_1:17486395-17486414 Msa0198800:CDS 25.0%
! GGAAACAAAAAGATTAAACA+AGG + chr2_1:17486464-17486483 Msa0198800:CDS 25.0%
! AGATTAAACAAGGAAATATC+AGG + chr2_1:17486474-17486493 Msa0198800:CDS 25.0%
! ATCTTGGTGAATATATTCAT+AGG - chr2_1:17487263-17487282 None:intergenic 25.0%
!!! CTTAAAGATGACTTTAGTTT+TGG - chr2_1:17487461-17487480 None:intergenic 25.0%
! CAAAACTAAAGTCATCTTTA+AGG + chr2_1:17487459-17487478 Msa0198800:CDS 25.0%
! TCTTGTATTTGTTTATTGCA+TGG + chr2_1:17487657-17487676 Msa0198800:three_prime_UTR 25.0%
TATCACTTTCAAATTGGCAT+TGG + chr2_1:17485756-17485775 Msa0198800:five_prime_UTR 30.0%
ATCACTTTCAAATTGGCATT+GGG + chr2_1:17485757-17485776 Msa0198800:five_prime_UTR 30.0%
CGAAATTAGTAAATTCAACG+TGG + chr2_1:17485783-17485802 Msa0198800:five_prime_UTR 30.0%
!! ACCTTTTAAACTATTTGCTG+AGG + chr2_1:17485837-17485856 Msa0198800:five_prime_UTR 30.0%
ACCTCAGCAAATAGTTTAAA+AGG - chr2_1:17485841-17485860 None:intergenic 30.0%
! TGCAAGAAAAGAATTTTGTG+TGG + chr2_1:17486259-17486278 Msa0198800:five_prime_UTR 30.0%
!! AAACATACTTGTTTCTAGCA+TGG + chr2_1:17486365-17486384 Msa0198800:CDS 30.0%
ATCTGAGTTAGAAAGTAAAG+TGG + chr2_1:17486428-17486447 Msa0198800:CDS 30.0%
GATGTGCAACGATTTAAAAA+GGG - chr2_1:17486552-17486571 None:intergenic 30.0%
TGATGTGCAACGATTTAAAA+AGG - chr2_1:17486553-17486572 None:intergenic 30.0%
ACATCAAATAACAGTGATAC+TGG + chr2_1:17486567-17486586 Msa0198800:CDS 30.0%
!! TACCTTTTCTGAGAAATTTG+TGG - chr2_1:17486679-17486698 None:intergenic 30.0%
ATAAGCAGATAGCAAAAGAA+CGG - chr2_1:17486711-17486730 None:intergenic 30.0%
TGACTATAACTATATCAGCT+TGG - chr2_1:17487118-17487137 None:intergenic 30.0%
TATAATGCAACTCGCTAATT+CGG - chr2_1:17487154-17487173 None:intergenic 30.0%
TGAATATATTCATAGGAGGA+AGG - chr2_1:17487256-17487275 None:intergenic 30.0%
TTGGTGAATATATTCATAGG+AGG - chr2_1:17487260-17487279 None:intergenic 30.0%
CAACAATAACATTCATGGAA+GGG - chr2_1:17487349-17487368 None:intergenic 30.0%
AGTAGATATTATGGTGTGTT+TGG + chr2_1:17487525-17487544 Msa0198800:three_prime_UTR 30.0%
TACATAATTCAGCTCAAAAG+TGG - chr2_1:17487772-17487791 None:intergenic 30.0%
TATCACTTTCAAATTGGCAT+TGG + chr2_1:17485756-17485775 Msa0198800:five_prime_UTR 30.0%
ATCACTTTCAAATTGGCATT+GGG + chr2_1:17485757-17485776 Msa0198800:five_prime_UTR 30.0%
CGAAATTAGTAAATTCAACG+TGG + chr2_1:17485783-17485802 Msa0198800:five_prime_UTR 30.0%
!! ACCTTTTAAACTATTTGCTG+AGG + chr2_1:17485837-17485856 Msa0198800:five_prime_UTR 30.0%
ACCTCAGCAAATAGTTTAAA+AGG - chr2_1:17485841-17485860 None:intergenic 30.0%
! TGCAAGAAAAGAATTTTGTG+TGG + chr2_1:17486259-17486278 Msa0198800:five_prime_UTR 30.0%
!! AAACATACTTGTTTCTAGCA+TGG + chr2_1:17486365-17486384 Msa0198800:CDS 30.0%
ATCTGAGTTAGAAAGTAAAG+TGG + chr2_1:17486428-17486447 Msa0198800:CDS 30.0%
GATGTGCAACGATTTAAAAA+GGG - chr2_1:17486552-17486571 None:intergenic 30.0%
TGATGTGCAACGATTTAAAA+AGG - chr2_1:17486553-17486572 None:intergenic 30.0%
ACATCAAATAACAGTGATAC+TGG + chr2_1:17486567-17486586 Msa0198800:CDS 30.0%
!! TACCTTTTCTGAGAAATTTG+TGG - chr2_1:17486679-17486698 None:intergenic 30.0%
ATAAGCAGATAGCAAAAGAA+CGG - chr2_1:17486711-17486730 None:intergenic 30.0%
TGACTATAACTATATCAGCT+TGG - chr2_1:17487118-17487137 None:intergenic 30.0%
TATAATGCAACTCGCTAATT+CGG - chr2_1:17487154-17487173 None:intergenic 30.0%
TGAATATATTCATAGGAGGA+AGG - chr2_1:17487256-17487275 None:intergenic 30.0%
TTGGTGAATATATTCATAGG+AGG - chr2_1:17487260-17487279 None:intergenic 30.0%
CAACAATAACATTCATGGAA+GGG - chr2_1:17487349-17487368 None:intergenic 30.0%
AGTAGATATTATGGTGTGTT+TGG + chr2_1:17487525-17487544 Msa0198800:three_prime_UTR 30.0%
TACATAATTCAGCTCAAAAG+TGG - chr2_1:17487772-17487791 None:intergenic 30.0%
! AGAGAGTACAAAGGATGATT+TGG + chr2_1:17485914-17485933 Msa0198800:five_prime_UTR 35.0%
!!! GATTTGGAGGATTTTAAAGC+CGG + chr2_1:17485930-17485949 Msa0198800:five_prime_UTR 35.0%
!!! ATTTGGAGGATTTTAAAGCC+GGG + chr2_1:17485931-17485950 Msa0198800:five_prime_UTR 35.0%
TTGAGAAAGGTAATCAAACC+CGG - chr2_1:17485952-17485971 None:intergenic 35.0%
!! CATTCGAGGATTTTTGAGAA+AGG - chr2_1:17485965-17485984 None:intergenic 35.0%
CTTTCTCAAAAATCCTCGAA+TGG + chr2_1:17485963-17485982 Msa0198800:five_prime_UTR 35.0%
AAAACATACTTCTCCATTCG+AGG - chr2_1:17485979-17485998 None:intergenic 35.0%
! CTGAGAAGTCACTTTATAAC+AGG + chr2_1:17486047-17486066 Msa0198800:intron 35.0%
AGAAAGTAAAGTGGCTGATA+TGG + chr2_1:17486437-17486456 Msa0198800:CDS 35.0%
AGTGACTCTTTCTTTGTTCA+GGG - chr2_1:17486628-17486647 None:intergenic 35.0%
TAGTGACTCTTTCTTTGTTC+AGG - chr2_1:17486629-17486648 None:intergenic 35.0%
!! CTTTTCTGAGAAATTTGTGG+AGG - chr2_1:17486676-17486695 None:intergenic 35.0%
CTCCACAAATTTCTCAGAAA+AGG + chr2_1:17486674-17486693 Msa0198800:CDS 35.0%
TAAGCAGATAGCAAAAGAAC+GGG - chr2_1:17486710-17486729 None:intergenic 35.0%
! AAACATGTGCTTGTTACCTT+TGG + chr2_1:17487048-17487067 Msa0198800:intron 35.0%
! AACATGTGCTTGTTACCTTT+GGG + chr2_1:17487049-17487068 Msa0198800:intron 35.0%
AAAAGCAGAATTTACCCCAA+AGG - chr2_1:17487067-17487086 None:intergenic 35.0%
ATAATGCAACTCGCTAATTC+GGG - chr2_1:17487153-17487172 None:intergenic 35.0%
GTTGCATTATATTGACCATC+TGG + chr2_1:17487163-17487182 Msa0198800:intron 35.0%
GCAACAATAACATTCATGGA+AGG - chr2_1:17487350-17487369 None:intergenic 35.0%
AAAAGAAGCTTGAAGAGGAA+AGG + chr2_1:17487377-17487396 Msa0198800:CDS 35.0%
! TCTCAGTAGTGGGAATAATT+GGG - chr2_1:17487406-17487425 None:intergenic 35.0%
AAGTTGGAAGAGAACAGTTT+GGG - chr2_1:17487431-17487450 None:intergenic 35.0%
!!! TAGTTTTGGAACCTTGAAGT+TGG - chr2_1:17487447-17487466 None:intergenic 35.0%
!! CTACTAGTTGTAGCTGTTTT+TGG - chr2_1:17487583-17487602 None:intergenic 35.0%
ACTGTGTGTGTTTGGAAATA+TGG - chr2_1:17487638-17487657 None:intergenic 35.0%
AATACAAGACTGTGTGTGTT+TGG - chr2_1:17487646-17487665 None:intergenic 35.0%
TGATTAGTGAGCTTTCTGTT+TGG + chr2_1:17487704-17487723 Msa0198800:three_prime_UTR 35.0%
! AGAGAGTACAAAGGATGATT+TGG + chr2_1:17485914-17485933 Msa0198800:five_prime_UTR 35.0%
!!! GATTTGGAGGATTTTAAAGC+CGG + chr2_1:17485930-17485949 Msa0198800:five_prime_UTR 35.0%
!!! ATTTGGAGGATTTTAAAGCC+GGG + chr2_1:17485931-17485950 Msa0198800:five_prime_UTR 35.0%
TTGAGAAAGGTAATCAAACC+CGG - chr2_1:17485952-17485971 None:intergenic 35.0%
!! CATTCGAGGATTTTTGAGAA+AGG - chr2_1:17485965-17485984 None:intergenic 35.0%
CTTTCTCAAAAATCCTCGAA+TGG + chr2_1:17485963-17485982 Msa0198800:five_prime_UTR 35.0%
AAAACATACTTCTCCATTCG+AGG - chr2_1:17485979-17485998 None:intergenic 35.0%
! CTGAGAAGTCACTTTATAAC+AGG + chr2_1:17486047-17486066 Msa0198800:intron 35.0%
AGAAAGTAAAGTGGCTGATA+TGG + chr2_1:17486437-17486456 Msa0198800:CDS 35.0%
AGTGACTCTTTCTTTGTTCA+GGG - chr2_1:17486628-17486647 None:intergenic 35.0%
TAGTGACTCTTTCTTTGTTC+AGG - chr2_1:17486629-17486648 None:intergenic 35.0%
!! CTTTTCTGAGAAATTTGTGG+AGG - chr2_1:17486676-17486695 None:intergenic 35.0%
CTCCACAAATTTCTCAGAAA+AGG + chr2_1:17486674-17486693 Msa0198800:CDS 35.0%
TAAGCAGATAGCAAAAGAAC+GGG - chr2_1:17486710-17486729 None:intergenic 35.0%
! AAACATGTGCTTGTTACCTT+TGG + chr2_1:17487048-17487067 Msa0198800:intron 35.0%
! AACATGTGCTTGTTACCTTT+GGG + chr2_1:17487049-17487068 Msa0198800:intron 35.0%
AAAAGCAGAATTTACCCCAA+AGG - chr2_1:17487067-17487086 None:intergenic 35.0%
ATAATGCAACTCGCTAATTC+GGG - chr2_1:17487153-17487172 None:intergenic 35.0%
GTTGCATTATATTGACCATC+TGG + chr2_1:17487163-17487182 Msa0198800:intron 35.0%
GCAACAATAACATTCATGGA+AGG - chr2_1:17487350-17487369 None:intergenic 35.0%
AAAAGAAGCTTGAAGAGGAA+AGG + chr2_1:17487377-17487396 Msa0198800:CDS 35.0%
! TCTCAGTAGTGGGAATAATT+GGG - chr2_1:17487406-17487425 None:intergenic 35.0%
AAGTTGGAAGAGAACAGTTT+GGG - chr2_1:17487431-17487450 None:intergenic 35.0%
!!! TAGTTTTGGAACCTTGAAGT+TGG - chr2_1:17487447-17487466 None:intergenic 35.0%
!! CTACTAGTTGTAGCTGTTTT+TGG - chr2_1:17487583-17487602 None:intergenic 35.0%
ACTGTGTGTGTTTGGAAATA+TGG - chr2_1:17487638-17487657 None:intergenic 35.0%
AATACAAGACTGTGTGTGTT+TGG - chr2_1:17487646-17487665 None:intergenic 35.0%
TGATTAGTGAGCTTTCTGTT+TGG + chr2_1:17487704-17487723 Msa0198800:three_prime_UTR 35.0%
! TTTGACTGCAGAGAGTACAA+AGG + chr2_1:17485905-17485924 Msa0198800:five_prime_UTR 40.0%
! GAGTACAAAGGATGATTTGG+AGG + chr2_1:17485917-17485936 Msa0198800:five_prime_UTR 40.0%
! GTCACTTTATAACAGGACCA+AGG + chr2_1:17486054-17486073 Msa0198800:intron 40.0%
AAGAAAGGAACCTCAAGTAG+TGG + chr2_1:17486105-17486124 Msa0198800:five_prime_UTR 40.0%
AGAAAGGAACCTCAAGTAGT+GGG + chr2_1:17486106-17486125 Msa0198800:five_prime_UTR 40.0%
! AGCTCATCTTTGGTTTCATC+AGG - chr2_1:17486174-17486193 None:intergenic 40.0%
!! CATACTTGTTTCTAGCATGG+AGG + chr2_1:17486368-17486387 Msa0198800:CDS 40.0%
TAAAGTGGCTGATATGGATG+CGG + chr2_1:17486443-17486462 Msa0198800:CDS 40.0%
! TTCTGAGAAATTTGTGGAGG+TGG - chr2_1:17486673-17486692 None:intergenic 40.0%
ACAGGGCTCTCTGTATTAAA+TGG + chr2_1:17486860-17486879 Msa0198800:intron 40.0%
! ACATGTGCTTGTTACCTTTG+GGG + chr2_1:17487050-17487069 Msa0198800:intron 40.0%
TATTCATAGGAGGAAGGAAC+AGG - chr2_1:17487250-17487269 None:intergenic 40.0%
CTGGGCAACAATAACATTCA+TGG - chr2_1:17487354-17487373 None:intergenic 40.0%
CATGAATGTTATTGTTGCCC+AGG + chr2_1:17487352-17487371 Msa0198800:intron 40.0%
! CTTCAAGCTTCTTTTCTCCT+GGG - chr2_1:17487372-17487391 None:intergenic 40.0%
! TCTTCAAGCTTCTTTTCTCC+TGG - chr2_1:17487373-17487392 None:intergenic 40.0%
AGGAGAAAAGAAGCTTGAAG+AGG + chr2_1:17487372-17487391 Msa0198800:intron 40.0%
! GTCTCAGTAGTGGGAATAAT+TGG - chr2_1:17487407-17487426 None:intergenic 40.0%
GAAGTTGGAAGAGAACAGTT+TGG - chr2_1:17487432-17487451 None:intergenic 40.0%
ACTGTTCTCTTCCAACTTCA+AGG + chr2_1:17487433-17487452 Msa0198800:CDS 40.0%
! TATGGTGTGTTTGGAACCAT+CGG + chr2_1:17487534-17487553 Msa0198800:three_prime_UTR 40.0%
!!! TTTGGAACCATCGGTTTTCA+CGG + chr2_1:17487543-17487562 Msa0198800:three_prime_UTR 40.0%
!!! GTAGCTGTTTTTGGTACGTT+AGG - chr2_1:17487574-17487593 None:intergenic 40.0%
! GTGAGCTTTCTGTTTGGAAT+AGG + chr2_1:17487710-17487729 Msa0198800:three_prime_UTR 40.0%
! TTTGACTGCAGAGAGTACAA+AGG + chr2_1:17485905-17485924 Msa0198800:five_prime_UTR 40.0%
! GAGTACAAAGGATGATTTGG+AGG + chr2_1:17485917-17485936 Msa0198800:five_prime_UTR 40.0%
! GTCACTTTATAACAGGACCA+AGG + chr2_1:17486054-17486073 Msa0198800:intron 40.0%
AAGAAAGGAACCTCAAGTAG+TGG + chr2_1:17486105-17486124 Msa0198800:five_prime_UTR 40.0%
AGAAAGGAACCTCAAGTAGT+GGG + chr2_1:17486106-17486125 Msa0198800:five_prime_UTR 40.0%
! AGCTCATCTTTGGTTTCATC+AGG - chr2_1:17486174-17486193 None:intergenic 40.0%
!! CATACTTGTTTCTAGCATGG+AGG + chr2_1:17486368-17486387 Msa0198800:CDS 40.0%
TAAAGTGGCTGATATGGATG+CGG + chr2_1:17486443-17486462 Msa0198800:CDS 40.0%
! TTCTGAGAAATTTGTGGAGG+TGG - chr2_1:17486673-17486692 None:intergenic 40.0%
ACAGGGCTCTCTGTATTAAA+TGG + chr2_1:17486860-17486879 Msa0198800:intron 40.0%
! ACATGTGCTTGTTACCTTTG+GGG + chr2_1:17487050-17487069 Msa0198800:intron 40.0%
TATTCATAGGAGGAAGGAAC+AGG - chr2_1:17487250-17487269 None:intergenic 40.0%
CTGGGCAACAATAACATTCA+TGG - chr2_1:17487354-17487373 None:intergenic 40.0%
CATGAATGTTATTGTTGCCC+AGG + chr2_1:17487352-17487371 Msa0198800:intron 40.0%
! CTTCAAGCTTCTTTTCTCCT+GGG - chr2_1:17487372-17487391 None:intergenic 40.0%
! TCTTCAAGCTTCTTTTCTCC+TGG - chr2_1:17487373-17487392 None:intergenic 40.0%
AGGAGAAAAGAAGCTTGAAG+AGG + chr2_1:17487372-17487391 Msa0198800:intron 40.0%
! GTCTCAGTAGTGGGAATAAT+TGG - chr2_1:17487407-17487426 None:intergenic 40.0%
GAAGTTGGAAGAGAACAGTT+TGG - chr2_1:17487432-17487451 None:intergenic 40.0%
ACTGTTCTCTTCCAACTTCA+AGG + chr2_1:17487433-17487452 Msa0198800:CDS 40.0%
! TATGGTGTGTTTGGAACCAT+CGG + chr2_1:17487534-17487553 Msa0198800:three_prime_UTR 40.0%
!!! TTTGGAACCATCGGTTTTCA+CGG + chr2_1:17487543-17487562 Msa0198800:three_prime_UTR 40.0%
!!! GTAGCTGTTTTTGGTACGTT+AGG - chr2_1:17487574-17487593 None:intergenic 40.0%
! GTGAGCTTTCTGTTTGGAAT+AGG + chr2_1:17487710-17487729 Msa0198800:three_prime_UTR 40.0%
TATCCTTCAATGGCGTTCCT+TGG - chr2_1:17486074-17486093 None:intergenic 45.0%
CTCTTGCCAGTATCCTTCAA+TGG - chr2_1:17486084-17486103 None:intergenic 45.0%
CATTGAAGGATACTGGCAAG+AGG + chr2_1:17486082-17486101 Msa0198800:five_prime_UTR 45.0%
GATACTGGCAAGAGGAAGAA+AGG + chr2_1:17486090-17486109 Msa0198800:five_prime_UTR 45.0%
CTTCTTCTTCCCACTACTTG+AGG - chr2_1:17486118-17486137 None:intergenic 45.0%
AAACCAAAGATGAGCTGAGC+AGG + chr2_1:17486178-17486197 Msa0198800:five_prime_UTR 45.0%
AACCAAAGATGAGCTGAGCA+GGG + chr2_1:17486179-17486198 Msa0198800:five_prime_UTR 45.0%
GGAAATATCAGGACTCTCAG+CGG + chr2_1:17486485-17486504 Msa0198800:CDS 45.0%
CTGGAAAAGTTGAGAGTAGC+AGG + chr2_1:17486586-17486605 Msa0198800:CDS 45.0%
GACTCTTTCTTTGTTCAGGG+TGG - chr2_1:17486625-17486644 None:intergenic 45.0%
! AGCTGTTGTAGTCTTGACAC+AGG + chr2_1:17486842-17486861 Msa0198800:intron 45.0%
! GCTGTTGTAGTCTTGACACA+GGG + chr2_1:17486843-17486862 Msa0198800:intron 45.0%
CAACTCGCTAATTCGGGAAT+AGG - chr2_1:17487147-17487166 None:intergenic 45.0%
! GCGTTGAAGCTATTTCCAGA+TGG - chr2_1:17487181-17487200 None:intergenic 45.0%
TAGGAGGAAGGAACAGGAAA+TGG - chr2_1:17487244-17487263 None:intergenic 45.0%
AGTTTGGGAGTCTCAGTAGT+GGG - chr2_1:17487416-17487435 None:intergenic 45.0%
GGTAAAGAGAGCTTAACGTG+CGG - chr2_1:17487617-17487636 None:intergenic 45.0%
TATCCTTCAATGGCGTTCCT+TGG - chr2_1:17486074-17486093 None:intergenic 45.0%
CTCTTGCCAGTATCCTTCAA+TGG - chr2_1:17486084-17486103 None:intergenic 45.0%
CATTGAAGGATACTGGCAAG+AGG + chr2_1:17486082-17486101 Msa0198800:five_prime_UTR 45.0%
GATACTGGCAAGAGGAAGAA+AGG + chr2_1:17486090-17486109 Msa0198800:five_prime_UTR 45.0%
CTTCTTCTTCCCACTACTTG+AGG - chr2_1:17486118-17486137 None:intergenic 45.0%
AAACCAAAGATGAGCTGAGC+AGG + chr2_1:17486178-17486197 Msa0198800:five_prime_UTR 45.0%
AACCAAAGATGAGCTGAGCA+GGG + chr2_1:17486179-17486198 Msa0198800:five_prime_UTR 45.0%
GGAAATATCAGGACTCTCAG+CGG + chr2_1:17486485-17486504 Msa0198800:CDS 45.0%
CTGGAAAAGTTGAGAGTAGC+AGG + chr2_1:17486586-17486605 Msa0198800:CDS 45.0%
GACTCTTTCTTTGTTCAGGG+TGG - chr2_1:17486625-17486644 None:intergenic 45.0%
! AGCTGTTGTAGTCTTGACAC+AGG + chr2_1:17486842-17486861 Msa0198800:intron 45.0%
! GCTGTTGTAGTCTTGACACA+GGG + chr2_1:17486843-17486862 Msa0198800:intron 45.0%
CAACTCGCTAATTCGGGAAT+AGG - chr2_1:17487147-17487166 None:intergenic 45.0%
! GCGTTGAAGCTATTTCCAGA+TGG - chr2_1:17487181-17487200 None:intergenic 45.0%
TAGGAGGAAGGAACAGGAAA+TGG - chr2_1:17487244-17487263 None:intergenic 45.0%
AGTTTGGGAGTCTCAGTAGT+GGG - chr2_1:17487416-17487435 None:intergenic 45.0%
GGTAAAGAGAGCTTAACGTG+CGG - chr2_1:17487617-17487636 None:intergenic 45.0%
!! AAAATAAAAAATAAATATCT+TGG - chr2_1:17487279-17487298 None:intergenic 5.0%
!! AAAATAAAAAATAAATATCT+TGG - chr2_1:17487279-17487298 None:intergenic 5.0%
GGAACGCCATTGAAGGATAC+TGG + chr2_1:17486075-17486094 Msa0198800:five_prime_UTR 50.0%
TACCCTGCTCAGCTCATCTT+TGG - chr2_1:17486184-17486203 None:intergenic 50.0%
! GAGGTGGAAGCATGTTCATC+AGG - chr2_1:17486657-17486676 None:intergenic 50.0%
CAGTTTGGGAGTCTCAGTAG+TGG - chr2_1:17487417-17487436 None:intergenic 50.0%
!!! AACCATCGGTTTTCACGGTG+TGG + chr2_1:17487548-17487567 Msa0198800:three_prime_UTR 50.0%
GGAACGCCATTGAAGGATAC+TGG + chr2_1:17486075-17486094 Msa0198800:five_prime_UTR 50.0%
TACCCTGCTCAGCTCATCTT+TGG - chr2_1:17486184-17486203 None:intergenic 50.0%
! GAGGTGGAAGCATGTTCATC+AGG - chr2_1:17486657-17486676 None:intergenic 50.0%
CAGTTTGGGAGTCTCAGTAG+TGG - chr2_1:17487417-17487436 None:intergenic 50.0%
!!! AACCATCGGTTTTCACGGTG+TGG + chr2_1:17487548-17487567 Msa0198800:three_prime_UTR 50.0%
GGACCAAGGAACGCCATTGA+AGG + chr2_1:17486068-17486087 Msa0198800:intron 55.0%
GGACCAAGGAACGCCATTGA+AGG + chr2_1:17486068-17486087 Msa0198800:intron 55.0%
GGCCACACCGTGAAAACCGA+TGG - chr2_1:17487553-17487572 None:intergenic 60.0%
GGCCACACCGTGAAAACCGA+TGG - chr2_1:17487553-17487572 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 17485749 17487814 17485749 ID=Msa0198800;Name=Msa0198800
chr2_1 mRNA 17485749 17487814 17485749 ID=Msa0198800-mRNA-1;Parent=Msa0198800;Name=Msa0198800-mRNA-1;_AED=0.00;_eAED=0.00;_QI=469|1|1|1|1|1|3|324|171
chr2_1 exon 17485749 17485989 17485749 ID=Msa0198800-mRNA-1:exon:7382;Parent=Msa0198800-mRNA-1
chr2_1 exon 17486069 17486695 17486069 ID=Msa0198800-mRNA-1:exon:7383;Parent=Msa0198800-mRNA-1
chr2_1 exon 17487374 17487814 17487374 ID=Msa0198800-mRNA-1:exon:7384;Parent=Msa0198800-mRNA-1
chr2_1 five_prime_UTR 17485749 17485989 17485749 ID=Msa0198800-mRNA-1:five_prime_utr;Parent=Msa0198800-mRNA-1
chr2_1 five_prime_UTR 17486069 17486296 17486069 ID=Msa0198800-mRNA-1:five_prime_utr;Parent=Msa0198800-mRNA-1
chr2_1 CDS 17486297 17486695 17486297 ID=Msa0198800-mRNA-1:cds;Parent=Msa0198800-mRNA-1
chr2_1 CDS 17487374 17487490 17487374 ID=Msa0198800-mRNA-1:cds;Parent=Msa0198800-mRNA-1
chr2_1 three_prime_UTR 17487491 17487814 17487491 ID=Msa0198800-mRNA-1:three_prime_utr;Parent=Msa0198800-mRNA-1
Gene Sequence

>Msa0198800

ATGGAGACTGACTACACTAAGAAATTGAGAAGTAAACAAGAAGAAGTAGAAAAGGCAAATGAGAAGATAAACATACTTGTTTCTAGCATGGAGGAAATGCAGTCTGAAAATATTAAAAAGGATAGTAGAATATCTGAGTTAGAAAGTAAAGTGGCTGATATGGATGCGGAAACAAAAAGATTAAACAAGGAAATATCAGGACTCTCAGCGGAATTGCAATCTTTAAGAAAGTTGAAGAGCAGCGAAGTTAAACCCTTTTTAAATCGTTGCACATCAAATAACAGTGATACTGGAAAAGTTGAGAGTAGCAGGAGTAGAAGAAACACCACCCTGAACAAAGAAAGAGTCACTAGTACTCCTGATGAACATGCTTCCACCTCCACAAATTTCTCAGAAAAGGAGAAAAGAAGCTTGAAGAGGAAAGGAAGCCCAATTATTCCCACTACTGAGACTCCCAAACTGTTCTCTTCCAACTTCAAGGTTCCAAAACTAAAGTCATCTTTAAGGAACAGATGA
Protein sequence

>Msa0198800

METDYTKKLRSKQEEVEKANEKINILVSSMEEMQSENIKKDSRISELESKVADMDAETKRLNKEISGLSAELQSLRKLKSSEVKPFLNRCTSNNSDTGKVESSRSRRNTTLNKERVTSTPDEHASTSTNFSEKEKRSLKRKGSPIIPTTETPKLFSSNFKVPKLKSSLRNR*