AlfalfaGEDB | Msa1024310

M. sativa cultivar zhongmu-4 / Msa1024310

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1024310	XP_003623274.1	84.615	117	8	2	1	113	1	111	3.54e-61	193

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1024310	G7KRF0	84.615	117	8	2	1	113	1	111	1.69e-61	193

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010150	Msa1024310	0.805041	1.616365e-49	-8.615850e-47
Msa0027860	Msa1024310	0.820944	5.248366e-53	-8.615850e-47
Msa0168010	Msa1024310	0.811438	7.001309e-51	-8.615850e-47
Msa0195190	Msa1024310	0.805805	1.117966e-49	-8.615850e-47
Msa0240580	Msa1024310	0.808802	2.589703e-50	-8.615850e-47
Msa0245660	Msa1024310	0.803231	3.847174e-49	-8.615850e-47
Msa0278960	Msa1024310	0.814295	1.657206e-51	-8.615850e-47
Msa0303900	Msa1024310	0.801008	1.102646e-48	-8.615850e-47
Msa0323090	Msa1024310	0.808880	2.491838e-50	-8.615850e-47
Msa0366510	Msa1024310	0.807344	5.291069e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1024310	MtrunA17_Chr7g0242141	84.615	117	8	2	1	113	1	111	3.25e-65	193
Msa1024310	MtrunA17_Chr7g0242081	51.961	102	43	3	30	126	27	127	4.27e-22	85.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 36 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGGGGATTCTCTTATAA+GGG	0.477382	7_1:+53383546	Msa1024310:CDS
ACAATGAATTGCACAAACAC+GGG	0.606791	7_1:+53383616	Msa1024310:CDS
ACCCGAATTGCTGACGATTC+AGG	0.254329	7_1:-53384886	None:intergenic
ACCCTGAATCGTCAGCAATT+CGG	0.349585	7_1:+53384884	Msa1024310:CDS
ACTCCATGCTCATCATCCGA+AGG	0.646422	7_1:-53383579	None:intergenic
AGCAATTCGGGTAAGTCTGG+AGG	0.657891	7_1:+53384897	Msa1024310:CDS
ATAACTTGGAAAGCAATTTC+AGG	0.384541	7_1:-53385038	None:intergenic
ATCGCGGTTTCTTGATTGGA+GGG	0.519025	7_1:-53383663	None:intergenic
ATCTCCGATCTTACAAATCA+AGG	0.478783	7_1:+53383684	Msa1024310:CDS
ATTTCAGGCCAAGTTTGCGA+AGG	0.621581	7_1:-53385023	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAGGGAAATAGTTATCCTT+CGG	+	chr7_1:53383563-53383582	Msa1024310:CDS	30.0%
TACAATGAATTGCACAAACA+CGG	+	chr7_1:53383615-53383634	Msa1024310:CDS	30.0%
CATGCAGAAAGTTGAAAATA+GGG	-	chr7_1:53383944-53383963	None:intergenic	30.0%
TCATGCAGAAAGTTGAAAAT+AGG	-	chr7_1:53383945-53383964	None:intergenic	30.0%
ATAACTTGGAAAGCAATTTC+AGG	-	chr7_1:53385041-53385060	None:intergenic	30.0%
TAAGGGAAATAGTTATCCTT+CGG	+	chr7_1:53383563-53383582	Msa1024310:CDS	30.0%
TACAATGAATTGCACAAACA+CGG	+	chr7_1:53383615-53383634	Msa1024310:CDS	30.0%
CATGCAGAAAGTTGAAAATA+GGG	-	chr7_1:53383944-53383963	None:intergenic	30.0%
TCATGCAGAAAGTTGAAAAT+AGG	-	chr7_1:53383945-53383964	None:intergenic	30.0%
ATAACTTGGAAAGCAATTTC+AGG	-	chr7_1:53385041-53385060	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	53383528	53385062	53383528	ID=Msa1024310;Name=Msa1024310
chr7_1	mRNA	53383528	53385062	53383528	ID=Msa1024310-mRNA-1;Parent=Msa1024310;Name=Msa1024310-mRNA-1;_AED=0.03;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|2\|0\|126
chr7_1	exon	53383528	53383720	53383528	ID=Msa1024310-mRNA-1:exon:10557;Parent=Msa1024310-mRNA-1
chr7_1	exon	53384875	53385062	53384875	ID=Msa1024310-mRNA-1:exon:10558;Parent=Msa1024310-mRNA-1
chr7_1	CDS	53383528	53383720	53383528	ID=Msa1024310-mRNA-1:cds;Parent=Msa1024310-mRNA-1
chr7_1	CDS	53384875	53385062	53384875	ID=Msa1024310-mRNA-1:cds;Parent=Msa1024310-mRNA-1

Gene Sequence	>Msa1024310 ATGGGTGAAAGCGATCATAAGAGGGGATTCTCTTATAAGGGAAATAGTTATCCTTCGGATGATGAGCATGGAGTCAAAAAAGCTAAGTACAATGAATTGCACAAACACGGGCGTAAACCAGAATCCAATTCTCCCCCCTCCAATCAAGAAACCGCGATCTCCGATCTTACAAATCAAGGATCAATAATAACAAAGAAATTCGACCCTGAATCGTCAGCAATTCGGGTAAGTCTGGAGGTGAACAAGGGAGAGTATAATCTGAATCTCAGAAGTGTTCCAACAGACAACGCGATGGAAGAGCATGCACACACCGCAGCTGTGTTGGTTTCTCAAGGAGAACTCCTTCGCAAACTTGGCCTGAAATTGCTTTCCAAGTTATGA
Protein sequence	>Msa1024310 MGESDHKRGFSYKGNSYPSDDEHGVKKAKYNELHKHGRKPESNSPPSNQETAISDLTNQGSIITKKFDPESSAIRVSLEVNKGEYNLNLRSVPTDNAMEEHAHTAAVLVSQGELLRKLGLKLLSKL*