AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0604280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0604280 MtrunA17_Chr4g0033341 96.140 544 21 0 1 544 1 544 0.0 1088
Msa0604280 MtrunA17_Chr4g0033311 68.217 516 160 2 31 543 1 515 0.0 752
Msa0604280 MtrunA17_Chr3g0088441 67.530 502 159 2 45 543 14 514 0.0 733
Msa0604280 MtrunA17_Chr4g0033331 67.540 496 155 3 51 541 19 513 0.0 718
Msa0604280 MtrunA17_Chr4g0033401 67.071 495 159 2 50 541 15 508 0.0 716
Msa0604280 MtrunA17_Chr4g0033321 66.534 502 161 4 45 541 8 507 0.0 714
Msa0604280 MtrunA17_Chr2g0329201 67.327 505 156 5 44 543 7 507 0.0 713
Msa0604280 MtrunA17_Chr2g0329191 68.172 487 150 3 60 543 25 509 0.0 711
Msa0604280 MtrunA17_Chr6g0482131 64.327 513 172 5 38 543 4 512 0.0 698
Msa0604280 MtrunA17_Chr6g0482071 62.428 519 180 5 34 543 1 513 0.0 677
Msa0604280 MtrunA17_Chr4g0033201 61.057 511 195 2 37 544 4 513 0.0 676
Msa0604280 MtrunA17_Chr4g0033191 62.828 495 177 3 56 544 27 520 0.0 670
Msa0604280 MtrunA17_Chr4g0033271 62.500 512 183 5 37 544 6 512 0.0 669
Msa0604280 MtrunA17_Chr4g0033261 62.573 513 163 4 33 543 8 493 0.0 664
Msa0604280 MtrunA17_Chr6g0482081 62.305 512 187 4 37 543 3 513 0.0 657
Msa0604280 MtrunA17_Chr4g0033231 60.878 501 170 3 46 544 18 494 0.0 651
Msa0604280 MtrunA17_Chr2g0329321 59.649 513 174 5 37 543 3 488 0.0 640
Msa0604280 MtrunA17_Chr5g0430451 56.084 526 190 6 31 543 1 498 0.0 580
Msa0604280 MtrunA17_Chr4g0006111 56.088 501 182 8 55 543 51 525 0.0 566
Msa0604280 MtrunA17_Chr4g0010501 55.230 478 206 4 68 543 23 494 0.0 560
Msa0604280 MtrunA17_Chr3g0087261 53.307 514 231 6 34 543 1 509 0.0 543
Msa0604280 MtrunA17_Chr4g0006131 54.902 510 193 9 46 544 19 502 0.0 540
Msa0604280 MtrunA17_Chr4g0043091 51.367 512 223 8 42 542 6 502 0.0 524
Msa0604280 MtrunA17_Chr3g0087271 51.845 515 241 5 31 543 1 510 2.97e-180 517
Msa0604280 MtrunA17_Chr4g0042171 48.047 512 242 8 42 542 1 499 6.24e-165 478
Msa0604280 MtrunA17_Chr6g0482941 48.622 508 248 8 40 543 14 512 2.40e-164 476
Msa0604280 MtrunA17_Chr6g0482951 47.233 506 258 6 40 543 14 512 2.44e-161 469
Msa0604280 MtrunA17_Chr2g0301231 53.957 417 157 7 137 543 1 392 3.00e-155 449
Msa0604280 MtrunA17_Chr3g0090131 46.960 477 242 5 66 542 18 483 5.77e-155 452
Msa0604280 MtrunA17_Chr3g0090111 46.624 474 241 6 69 542 19 480 1.55e-152 446
Msa0604280 MtrunA17_Chr4g0074561 45.436 493 250 6 55 541 31 510 3.52e-150 441
Msa0604280 MtrunA17_Chr4g0074551 48.000 475 239 6 74 544 55 525 6.07e-149 439
Msa0604280 MtrunA17_Chr2g0288071 46.751 477 247 5 72 544 70 543 8.93e-149 438
Msa0604280 MtrunA17_Chr2g0288021 44.358 514 272 8 37 544 5 510 2.60e-142 421
Msa0604280 MtrunA17_Chr5g0428831 44.685 508 249 11 43 542 6 489 2.16e-140 416
Msa0604280 MtrunA17_Chr7g0242691 44.398 482 246 8 68 543 30 495 6.45e-140 414
Msa0604280 MtrunA17_Chr7g0242681 45.979 485 229 11 69 542 38 500 6.84e-140 415
Msa0604280 MtrunA17_Chr1g0195611 45.079 508 246 10 43 542 6 488 1.40e-139 413
Msa0604280 MtrunA17_Chr2g0317821 42.105 513 275 8 36 541 6 503 7.81e-139 412
Msa0604280 MtrunA17_Chr2g0317841 41.715 513 277 8 36 541 6 503 1.94e-138 411
Msa0604280 MtrunA17_Chr4g0006971 45.094 479 241 9 69 541 27 489 9.09e-134 399
Msa0604280 MtrunA17_Chr7g0234131 43.882 474 247 6 70 541 76 532 8.11e-123 371
Msa0604280 MtrunA17_Chr7g0234091 43.396 477 250 7 70 544 76 534 1.50e-122 370
Msa0604280 MtrunA17_Chr7g0234111 43.277 476 252 5 70 544 76 534 2.35e-121 367
Msa0604280 MtrunA17_Chr3g0087201 53.293 334 147 4 213 543 17 344 3.06e-119 355
Msa0604280 MtrunA17_Chr5g0418871 56.623 302 124 4 88 386 2 299 5.51e-119 354
Msa0604280 MtrunA17_Chr1g0195641 47.449 392 187 7 68 453 26 404 1.07e-113 343
Msa0604280 MtrunA17_Chr7g0230651 42.895 373 199 5 116 488 18 376 4.85e-97 300
Msa0604280 MtrunA17_Chr3g0087191 67.586 145 47 0 68 212 21 165 1.08e-70 224
Msa0604280 MtrunA17_Chr7g0227561 38.007 271 129 5 71 337 48 283 3.74e-59 198
Msa0604280 MtrunA17_Chr7g0236731 46.250 240 124 3 158 396 28 263 4.43e-59 197
Msa0604280 MtrunA17_Chr4g0068871 25.754 431 238 13 110 533 169 524 2.01e-24 107
Msa0604280 MtrunA17_Chr5g0418861 52.222 90 42 1 453 541 1 90 6.03e-24 95.9
Msa0604280 MtrunA17_Chr8g0352881 45.833 96 51 1 293 388 58 152 8.82e-20 86.7
Msa0604280 MtrunA17_Chr8g0352881 50.000 54 26 1 78 131 7 59 2.34e-11 62.4
Msa0604280 MtrunA17_Chr2g0288031 54.237 59 25 1 221 277 4 62 3.27e-12 61.6
Msa0604280 MtrunA17_Chr7g0227591 52.727 55 26 0 490 544 14 68 5.70e-12 61.2
Msa0604280 MtrunA17_Chr1g0195651 54.902 51 22 1 493 542 1 51 2.74e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0604280 AT5G44640.1 53.876 516 218 8 36 544 5 507 0.0 566
Msa0604280 AT5G42260.1 54.473 503 214 7 39 537 6 497 0.0 558
Msa0604280 AT2G44450.1 55.165 484 204 5 61 541 27 500 0.0 554
Msa0604280 AT2G44480.1 54.412 476 214 2 68 541 34 508 0.0 543
Msa0604280 AT2G44480.3 54.412 476 214 2 68 541 65 539 0.0 543
Msa0604280 AT3G60130.1 53.556 478 212 5 69 542 30 501 0.0 527
Msa0604280 AT2G25630.1 51.569 510 212 8 36 541 5 483 0.0 519
Msa0604280 AT1G26560.1 53.222 481 215 6 67 541 30 506 1.97e-176 508
Msa0604280 AT5G24550.1 48.256 516 251 7 39 544 3 512 1.15e-174 504
Msa0604280 AT3G60140.1 48.560 486 236 5 68 544 25 505 2.45e-171 497
Msa0604280 AT3G18080.1 51.235 486 223 8 64 544 34 510 2.81e-171 494
Msa0604280 AT3G60120.1 50.728 481 230 5 68 543 13 491 4.41e-171 495
Msa0604280 AT5G54570.1 49.897 485 224 6 68 541 29 505 5.78e-170 492
Msa0604280 AT5G24540.1 48.269 520 242 7 39 544 6 512 4.46e-169 490
Msa0604280 AT1G51470.1 49.053 475 234 5 69 543 45 511 1.37e-167 485
Msa0604280 AT2G44490.1 49.687 479 233 5 71 542 16 493 8.26e-167 485
Msa0604280 AT1G47600.1 49.057 477 231 6 69 543 45 511 8.94e-166 480
Msa0604280 AT1G47600.2 48.847 477 231 6 69 543 45 510 1.43e-165 480
Msa0604280 AT2G44460.1 47.151 509 253 7 42 541 3 504 1.11e-162 475
Msa0604280 AT3G18070.3 48.466 489 230 9 61 544 22 493 1.96e-162 471
Msa0604280 AT3G18070.1 48.671 489 235 9 61 544 22 499 2.60e-161 469
Msa0604280 AT2G44470.3 44.727 512 273 5 37 543 1 507 2.62e-160 469
Msa0604280 AT2G44480.2 52.826 407 189 2 137 541 1 406 1.40e-157 456
Msa0604280 AT2G32860.1 48.017 479 241 5 69 541 94 570 2.21e-155 457
Msa0604280 AT3G60130.2 53.125 416 185 5 131 542 40 449 2.52e-155 452
Msa0604280 AT5G25980.2 46.080 523 262 12 30 542 11 523 4.08e-155 454
Msa0604280 AT2G32860.2 47.490 478 246 3 69 541 94 571 3.46e-154 454
Msa0604280 AT3G09260.1 46.458 480 250 5 69 543 35 512 1.25e-153 450
Msa0604280 AT1G75940.1 46.307 501 255 8 51 541 21 517 5.33e-153 449
Msa0604280 AT5G36890.2 46.835 474 239 6 71 542 18 480 9.96e-152 444
Msa0604280 AT5G36890.1 46.835 474 239 6 71 542 18 480 1.17e-151 444
Msa0604280 AT5G54570.3 50.116 431 197 5 68 488 29 451 2.13e-150 439
Msa0604280 AT3G03640.1 44.444 513 273 7 39 543 6 514 7.57e-150 441
Msa0604280 AT1G66280.1 45.000 500 263 7 50 541 15 510 2.26e-148 436
Msa0604280 AT1G66270.1 44.779 498 259 7 58 543 19 512 2.92e-147 434
Msa0604280 AT3G60130.3 51.923 416 179 6 131 542 40 438 4.72e-147 431
Msa0604280 AT3G21370.1 45.267 486 248 8 69 542 33 512 2.26e-146 431
Msa0604280 AT5G28510.1 43.107 515 279 7 37 541 9 519 4.52e-146 431
Msa0604280 AT5G24540.2 47.619 462 216 6 39 487 6 454 5.70e-146 428
Msa0604280 AT1G52400.3 45.224 513 266 9 39 542 10 516 8.50e-146 430
Msa0604280 AT1G52400.1 45.224 513 266 9 39 542 10 516 8.50e-146 430
Msa0604280 AT1G02850.2 47.107 484 221 10 70 542 27 486 9.59e-146 429
Msa0604280 AT5G26000.1 47.808 479 231 10 73 541 42 511 1.08e-144 428
Msa0604280 AT1G66270.2 44.378 498 259 8 58 543 19 510 8.81e-144 425
Msa0604280 AT2G44470.1 44.835 455 242 4 37 487 1 450 2.13e-141 416
Msa0604280 AT1G02850.5 45.168 507 220 11 70 542 27 509 3.97e-141 418
Msa0604280 AT5G25980.3 46.039 467 234 10 30 488 11 467 9.15e-140 412
Msa0604280 AT5G25980.1 46.039 467 234 10 30 488 11 467 9.15e-140 412
Msa0604280 AT1G61820.1 45.397 478 253 7 72 544 33 507 1.05e-139 414
Msa0604280 AT1G02850.4 45.276 508 219 12 70 542 27 510 2.26e-139 413
Msa0604280 AT3G60140.2 48.615 397 191 4 68 456 25 416 4.15e-138 408
Msa0604280 AT4G27830.1 44.099 483 247 8 65 541 19 484 1.44e-137 408
Msa0604280 AT1G60090.2 43.590 507 254 12 41 542 4 483 1.19e-136 406
Msa0604280 AT4G27820.1 45.833 480 233 9 69 541 23 482 2.68e-136 405
Msa0604280 AT4G22100.1 44.259 479 245 8 71 544 24 485 1.58e-135 403
Msa0604280 AT1G02850.1 45.248 484 203 10 70 542 27 459 3.32e-134 398
Msa0604280 AT5G54570.2 47.596 416 199 6 137 541 1 408 6.18e-134 397
Msa0604280 AT1G60090.1 42.969 512 257 13 41 542 4 490 6.52e-134 399
Msa0604280 AT2G44450.2 53.521 355 156 4 190 541 1 349 8.06e-134 393
Msa0604280 AT1G45191.4 44.538 476 240 8 70 542 30 484 8.51e-133 396
Msa0604280 AT4G21760.2 40.226 532 288 10 18 541 12 521 3.91e-132 395
Msa0604280 AT1G45191.2 43.724 478 247 8 70 542 30 490 5.63e-131 392
Msa0604280 AT1G61810.1 44.075 481 261 7 69 544 33 510 9.90e-130 389
Msa0604280 AT5G26000.2 47.877 424 204 8 73 488 42 456 1.05e-129 386
Msa0604280 AT1G45191.6 43.505 485 243 9 70 542 30 495 1.47e-129 388
Msa0604280 AT2G44480.4 58.654 312 125 3 68 377 65 374 1.99e-129 383
Msa0604280 AT4G21760.1 39.662 532 281 11 18 541 12 511 4.99e-127 382
Msa0604280 AT1G52400.2 45.295 457 236 8 39 487 10 460 6.89e-127 379
Msa0604280 AT1G45191.1 42.886 492 248 10 70 542 30 507 7.35e-127 382
Msa0604280 AT3G62750.1 41.458 480 251 10 66 541 20 473 9.28e-125 375
Msa0604280 AT3G62750.2 41.458 480 251 10 66 541 46 499 1.81e-124 375
Msa0604280 AT1G61810.3 43.871 465 253 7 69 528 33 494 1.71e-122 371
Msa0604280 AT2G44470.2 44.361 399 213 4 37 431 1 394 1.75e-120 360
Msa0604280 AT3G62740.1 40.041 487 269 9 58 541 12 478 1.10e-118 360
Msa0604280 AT1G02850.3 46.394 416 172 9 70 452 27 424 2.64e-117 354
Msa0604280 AT5G48375.1 44.218 441 170 11 69 503 38 408 4.22e-115 348
Msa0604280 AT3G18070.4 51.682 327 150 5 61 385 22 342 8.43e-113 340
Msa0604280 AT1G60270.1 46.791 374 183 7 41 410 1 362 4.35e-111 336
Msa0604280 AT4G27830.2 43.066 411 213 7 137 541 1 396 1.22e-109 333
Msa0604280 AT4G27820.2 44.417 412 207 8 137 541 1 397 1.26e-107 328
Msa0604280 AT4G27820.3 44.417 412 207 8 137 541 1 397 1.26e-107 328
Msa0604280 AT2G44460.2 47.107 363 178 5 188 541 1 358 9.37e-107 327
Msa0604280 AT3G62750.4 40.553 434 229 9 66 496 46 453 3.31e-106 326
Msa0604280 AT1G51490.1 47.246 345 171 5 76 419 27 361 3.51e-105 322
Msa0604280 AT1G45191.3 47.761 335 163 5 70 400 30 356 5.05e-105 322
Msa0604280 AT1G45191.5 47.761 335 163 5 70 400 30 356 7.24e-105 321
Msa0604280 AT1G61810.5 43.627 408 222 7 142 544 6 410 6.31e-104 319
Msa0604280 AT1G61810.4 44.473 389 209 6 69 452 33 419 2.60e-103 317
Msa0604280 AT3G62740.2 39.815 432 238 8 58 487 12 423 1.25e-101 313
Msa0604280 AT3G18070.2 47.720 329 161 6 221 544 100 422 2.71e-100 310
Msa0604280 AT3G18070.2 54.930 71 31 1 61 131 22 91 8.70e-18 86.3
Msa0604280 AT2G44480.5 48.515 303 153 2 241 541 1 302 1.42e-98 301
Msa0604280 AT3G62750.7 43.860 342 172 6 66 407 20 341 2.12e-97 300
Msa0604280 AT3G62750.8 43.860 342 172 6 66 407 20 341 2.12e-97 300
Msa0604280 AT3G62750.6 39.853 409 221 9 137 541 1 388 1.06e-96 300
Msa0604280 AT3G62750.5 39.853 409 221 9 137 541 1 388 1.06e-96 300
Msa0604280 AT3G62750.3 43.860 342 172 6 66 407 46 367 2.39e-96 300
Msa0604280 AT1G61820.3 43.324 367 201 6 183 544 4 368 1.03e-94 294
Msa0604280 AT3G18070.7 47.152 316 156 6 234 544 1 310 1.23e-94 291
Msa0604280 AT1G61810.2 50.562 267 128 3 72 336 36 300 5.49e-86 269
Msa0604280 AT1G60270.2 47.482 278 139 4 137 410 1 275 1.02e-81 257
Msa0604280 AT3G18070.5 47.699 239 117 4 221 454 100 335 3.07e-68 224
Msa0604280 AT3G18070.5 54.930 71 31 1 61 131 22 91 4.80e-18 86.3
Msa0604280 AT3G18070.6 47.111 225 111 4 234 453 1 222 1.26e-62 205
Msa0604280 AT5G16580.1 38.811 286 160 4 215 498 27 299 4.00e-52 180
Msa0604280 AT3G06510.1 25.714 420 235 14 132 542 159 510 7.07e-26 112
Msa0604280 AT3G06510.2 25.173 433 255 13 132 542 159 544 3.38e-24 107

Find 127 sgRNAs with CRISPR-Local

Find 460 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTTAATTTCAGGTTTA+TGG 0.066072 4_2:-49735089 Msa0604280:intron
TAGCCAATGAATACCATATT+TGG 0.163685 4_2:+49741657 None:intergenic
TTTCCACGTGGCTTCATCTT+TGG 0.183969 4_2:-49741522 Msa0604280:CDS
TGATTATGTGTAACTATATA+AGG 0.212347 4_2:+49737595 None:intergenic
TTCCACGTGGCTTCATCTTT+GGG 0.217307 4_2:-49741521 Msa0604280:CDS
ACATGTTTGGGTGGTAATTC+TGG 0.227903 4_2:-49737625 Msa0604280:CDS
ACAATTGCAGTTGAACAAAA+TGG 0.236440 4_2:-49734751 Msa0604280:intron
GACTTACTTGGGAGTATTCT+TGG 0.244929 4_2:+49738240 None:intergenic
AGATCACTTGACTTCCAATT+TGG 0.245781 4_2:-49737318 Msa0604280:CDS
ATATATAGGCTGCTTCAATT+TGG 0.265595 4_2:-49734555 Msa0604280:intron
TTCTGTAGAAGAAGCTCTTT+TGG 0.269060 4_2:-49734244 Msa0604280:CDS
TAATTAATTACCATCCAAAT+TGG 0.278065 4_2:+49737304 None:intergenic
CATTGCCTTGTGGAGGTAAA+TGG 0.282756 4_2:+49734921 None:intergenic
GCATAACATTGGTAACAAAT+TGG 0.283831 4_2:-49737389 Msa0604280:CDS
GACTGTAATGAATGGTTTGC+AGG 0.311829 4_2:-49733962 Msa0604280:CDS
ACAAGCATATGGATCGTAAT+AGG 0.315574 4_2:-49741749 None:intergenic
GCAGGCTTTACTGTTCGTTT+TGG 0.320577 4_2:-49733944 Msa0604280:CDS
GTGGTAGAGGACCAAGTATC+TGG 0.325786 4_2:-49740900 Msa0604280:CDS
ATGTTCTAGCCCAACATGTT+TGG 0.329686 4_2:-49737638 Msa0604280:CDS
CAAGCATATGGATCGTAATA+GGG 0.335114 4_2:-49741748 None:intergenic
GATTATGAAGGATCAGAATA+TGG 0.342816 4_2:-49738289 Msa0604280:CDS
GTGGTCTACATTACTGAAAA+TGG 0.345716 4_2:-49734466 Msa0604280:intron
GATCTTTGCTTCAAGGAATT+CGG 0.349501 4_2:-49737748 Msa0604280:CDS
TACCATTGAGCAGAAAGCTT+TGG 0.350960 4_2:+49733881 None:intergenic
CAAGCAGCACAAACCAAATA+TGG 0.360644 4_2:-49741670 Msa0604280:CDS
CTATGCTGATCTTTGCTTCA+AGG 0.368377 4_2:-49737755 Msa0604280:CDS
TGTTCTAGCCCAACATGTTT+GGG 0.369906 4_2:-49737637 Msa0604280:CDS
GGATAATCTCCGGTTGTTAG+AGG 0.377413 4_2:+49735064 None:intergenic
ACCATTGAGCAGAAAGCTTT+GGG 0.379817 4_2:+49733882 None:intergenic
GCATATCAAAAGGGCAAGAT+AGG 0.386935 4_2:-49737411 Msa0604280:CDS
AACTTTGTAGATTACAATGA+TGG 0.387322 4_2:-49733917 Msa0604280:CDS
TGACCAATACCATCGCTATA+AGG 0.400152 4_2:-49739478 Msa0604280:intron
AAAAGATCTCGAAGTCCTCT+TGG 0.415218 4_2:+49734520 None:intergenic
CGTGGCTTCATCTTTGGGGC+GGG 0.423208 4_2:-49741516 Msa0604280:CDS
TCGGAGATAGAGTGAAGTAC+TGG 0.425135 4_2:-49737729 Msa0604280:CDS
ATACTCGTCTTCTAAGACTT+GGG 0.426002 4_2:+49737898 None:intergenic
TGTGTATAAGATCAATTATC+AGG 0.433730 4_2:-49737545 Msa0604280:intron
CAACCTCATCAACGAATTGT+TGG 0.435996 4_2:-49738078 Msa0604280:CDS
AAGTGTGCAGGCATATCAAA+AGG 0.439406 4_2:-49737421 Msa0604280:intron
ATCAACGAATTGTTGGCTAA+TGG 0.451216 4_2:-49738071 Msa0604280:intron
AAACCAAATATGGTATTCAT+TGG 0.452074 4_2:-49741660 Msa0604280:CDS
CAATAGCATACCCGATCAAA+AGG 0.455559 4_2:-49737349 Msa0604280:CDS
GCACCAGCTTCAAACAATAT+AGG 0.456067 4_2:+49733832 None:intergenic
ATTTAGTGAACTTAGGCAAT+CGG 0.457331 4_2:+49735014 None:intergenic
TTGGAAGTCAAGTGATCTCT+TGG 0.460981 4_2:+49737323 None:intergenic
CAAACATGTATATAGTTTGA+AGG 0.469332 4_2:-49740937 Msa0604280:intron
TATTACTACAGGAAGATGTT+GGG 0.470013 4_2:-49738311 Msa0604280:intron
TTATTACTACAGGAAGATGT+TGG 0.473846 4_2:-49738312 Msa0604280:intron
CCTATAGATTCTCAATCGCT+TGG 0.475831 4_2:-49738263 Msa0604280:CDS
CAAGTCTTAGAAGACGAGTA+TGG 0.476058 4_2:-49737896 Msa0604280:CDS
ATAGTTTGAAGGTGCAGTAA+AGG 0.476392 4_2:-49740926 Msa0604280:intron
GCAGTAAAGGAAGGTGGTAG+AGG 0.488218 4_2:-49740913 Msa0604280:CDS
ACCCAAAGCTTTCTGCTCAA+TGG 0.488244 4_2:-49733883 Msa0604280:CDS
TTACATGAAGTATTAGTCAT+AGG 0.489885 4_2:+49734875 None:intergenic
CCAAGCGATTGAGAATCTAT+AGG 0.495495 4_2:+49738263 None:intergenic
ATAGGATCTGTTAAGTAACT+TGG 0.496012 4_2:+49734893 None:intergenic
CAGGGCCGGCTCGAATGTAA+AGG 0.500785 4_2:-49734008 Msa0604280:CDS
GATTGCAATTTAGTGAACTT+AGG 0.503385 4_2:+49735007 None:intergenic
GTGAAGGTGTCCCAGATACT+TGG 0.503819 4_2:+49740889 None:intergenic
CATACTCGTCTTCTAAGACT+TGG 0.503889 4_2:+49737897 None:intergenic
AGTAATGGTGGATATGCAAT+GGG 0.504655 4_2:-49737679 Msa0604280:CDS
CCATGATCATATATATTAGT+TGG 0.505953 4_2:-49733721 Msa0604280:three_prime_UTR
AATTACCACCCAAACATGTT+GGG 0.506442 4_2:+49737629 None:intergenic
GTACCTTATAGCGATGGTAT+TGG 0.507757 4_2:+49739475 None:intergenic
GTCACACTTACTTTCGCAAA+CGG 0.507884 4_2:-49741594 Msa0604280:CDS
CACTTGACTTCCAATTTGGA+TGG 0.509427 4_2:-49737314 Msa0604280:intron
TCCACGTGGCTTCATCTTTG+GGG 0.509644 4_2:-49741520 Msa0604280:CDS
AGTCCTATATTGTTTGAAGC+TGG 0.511064 4_2:-49733835 Msa0604280:three_prime_UTR
ACAAATTGGTTCATACCACT+TGG 0.515567 4_2:-49737375 Msa0604280:CDS
TTTATGGAACCTCTAACAAC+CGG 0.516409 4_2:-49735073 Msa0604280:CDS
AAAATCAAACTGATATATGA+AGG 0.518983 4_2:+49733697 None:intergenic
CCAACTAATATATATGATCA+TGG 0.521748 4_2:+49733721 None:intergenic
AGGGCCGGCTCGAATGTAAA+GGG 0.522499 4_2:-49734007 Msa0604280:CDS
GTGGAAAACTACTCCGACTC+AGG 0.522539 4_2:+49741538 None:intergenic
TTGCAATCAAAGCTAGTGAA+TGG 0.522902 4_2:-49734992 Msa0604280:CDS
GAATTACCACCCAAACATGT+TGG 0.526460 4_2:+49737628 None:intergenic
TGATCATGGCTGCTGGTACA+AGG 0.528228 4_2:+49733735 None:intergenic
GAGCCATGGTTGTTTAGTAA+TGG 0.533702 4_2:-49737694 Msa0604280:CDS
GTCTGCCTCTTTATACATGA+AGG 0.537626 4_2:+49741717 None:intergenic
CCATGGTTGTTTAGTAATGG+TGG 0.540507 4_2:-49737691 Msa0604280:CDS
GGATCTGTTAAGTAACTTGG+AGG 0.542167 4_2:+49734896 None:intergenic
ACCTTCACCCATCAACATCC+AGG 0.542518 4_2:-49740874 Msa0604280:intron
ATGGAAAACCTCTAGGTCCA+AGG 0.542893 4_2:-49734732 Msa0604280:CDS
ATATATATGATCATGGCTGC+TGG 0.547638 4_2:+49733728 None:intergenic
AACAACAAAGAGAGCAAGCA+TGG 0.551044 4_2:+49741623 None:intergenic
ATTACAATGATGGATTGAAA+AGG 0.551309 4_2:-49733907 Msa0604280:CDS
AAAATAGAGGGGAAGTTGAG+TGG 0.552316 4_2:-49738134 Msa0604280:intron
GGGTTACATTTAACGAGCCA+TGG 0.553181 4_2:-49737708 Msa0604280:CDS
GAACAAAATGGAAAACCTCT+AGG 0.554211 4_2:-49734739 Msa0604280:CDS
GAAAGATTATTACTTGATTG+CGG 0.555386 4_2:+49734173 None:intergenic
TAGCCAACAATTCGTTGATG+AGG 0.556574 4_2:+49738075 None:intergenic
TTTGAAGGTGCAGTAAAGGA+AGG 0.559649 4_2:-49740922 Msa0604280:CDS
GTCCTCCTTCATGTATAAAG+AGG 0.565539 4_2:-49741722 Msa0604280:CDS
ACGTGGCTTCATCTTTGGGG+CGG 0.566913 4_2:-49741517 Msa0604280:CDS
TGGTAGAGGACCAAGTATCT+GGG 0.567259 4_2:-49740899 Msa0604280:CDS
GAAGGTGCAGTAAAGGAAGG+TGG 0.572564 4_2:-49740919 Msa0604280:CDS
AAGCATATGGATCGTAATAG+GGG 0.574021 4_2:-49741747 None:intergenic
TTGACATCGGCAACCTGAGT+CGG 0.575732 4_2:-49741551 Msa0604280:CDS
CTCAGGTTGCCGATGTCAAG+AGG 0.581408 4_2:+49741555 None:intergenic
AAATCCCTTTACATTCGAGC+CGG 0.581654 4_2:+49734003 None:intergenic
TGGAAAACCTCTAGGTCCAA+GGG 0.581920 4_2:-49734731 Msa0604280:intron
GGAAGATGTTGGGATTATGA+AGG 0.590173 4_2:-49738301 Msa0604280:CDS
CGGAGATAGAGTGAAGTACT+GGG 0.599926 4_2:-49737728 Msa0604280:CDS
AGTGTGCAGGCATATCAAAA+GGG 0.608518 4_2:-49737420 Msa0604280:intron
CTTGGAGGAGCATTGCCTTG+TGG 0.612202 4_2:+49734911 None:intergenic
GCAATGGGAACAACAGCACC+AGG 0.613253 4_2:-49737664 Msa0604280:CDS
GAGCTAGTACCTTATAGCGA+TGG 0.616078 4_2:+49739469 None:intergenic
TGGATATACGTTTATCCAAG+AGG 0.618701 4_2:-49734535 Msa0604280:CDS
AGTGGAGGCATAAATCATGA+AGG 0.618703 4_2:-49738116 Msa0604280:CDS
GGAGGAGCATTGCCTTGTGG+AGG 0.623056 4_2:+49734914 None:intergenic
GGGCAAGATAGGCATAACAT+TGG 0.623136 4_2:-49737400 Msa0604280:CDS
ACCTGGATGTTGATGGGTGA+AGG 0.626176 4_2:+49740873 None:intergenic
TACTCGTCTTCTAAGACTTG+GGG 0.626972 4_2:+49737899 None:intergenic
AGACTTACCCTTGGACCTAG+AGG 0.628477 4_2:+49734724 None:intergenic
GTTTCAACTCCTCTTGACAT+CGG 0.629562 4_2:-49741564 Msa0604280:CDS
TAGTAATGGTGGATATGCAA+TGG 0.631646 4_2:-49737680 Msa0604280:CDS
GTCTTAGAAGACGAGTATGG+TGG 0.639976 4_2:-49737893 Msa0604280:CDS
ATAGAGGGGAAGTTGAGTGG+AGG 0.640276 4_2:-49738131 Msa0604280:intron
ATTCATTGGCTATTTCAATG+TGG 0.649386 4_2:-49741646 Msa0604280:CDS
TCTAGCCCAACATGTTTGGG+TGG 0.652575 4_2:-49737634 Msa0604280:CDS
GCCCCAAAGATGAAGCCACG+TGG 0.674470 4_2:+49741519 None:intergenic
TGCCTCTTTATACATGAAGG+AGG 0.679710 4_2:+49741720 None:intergenic
ATTAACCATTTACCTCCACA+AGG 0.685327 4_2:-49734926 Msa0604280:CDS
CCACCATTACTAAACAACCA+TGG 0.686919 4_2:+49737691 None:intergenic
GGTATGCTATTGTTTCCAAG+TGG 0.687798 4_2:+49737360 None:intergenic
GGAGAAATACAACAATCCTG+TGG 0.690499 4_2:-49734485 Msa0604280:CDS
GTTGGGCTAGAACATCGACC+TGG 0.733866 4_2:+49737646 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAAAAACC+TGG + chr4_2:49734543-49734562 None:intergenic 10.0%
!!! TTTTTTTGTTTTTTTTTTAC+CGG - chr4_2:49734961-49734980 Msa0604280:CDS 10.0%
!! CTTTATTAAAAAGTTTAATT+TGG + chr4_2:49735366-49735385 None:intergenic 10.0%
!!! TTTTAAATGTAGAAAAAATA+AGG - chr4_2:49735869-49735888 Msa0604280:intron 10.0%
!! TTTAAATGTAGAAAAAATAA+GGG - chr4_2:49735870-49735889 Msa0604280:intron 10.0%
!! TAATAATTCCTATTTAATAT+AGG + chr4_2:49735956-49735975 None:intergenic 10.0%
!! TATATTTAATGATAACTTAA+GGG - chr4_2:49736001-49736020 Msa0604280:intron 10.0%
!! AAAATCCTATTTATTATTAT+AGG + chr4_2:49736036-49736055 None:intergenic 10.0%
!!! AATAAAAAATTTGTTGTTTT+CGG + chr4_2:49736063-49736082 None:intergenic 10.0%
!! TTATATTATATATAATTTGG+AGG + chr4_2:49736501-49736520 None:intergenic 10.0%
!! AAGAATTAAAAAAGAATTAA+TGG - chr4_2:49736692-49736711 Msa0604280:intron 10.0%
!! TAATATATGAAAATTATACA+TGG + chr4_2:49736814-49736833 None:intergenic 10.0%
!!! AATTAATTATTGATTTGTTT+TGG - chr4_2:49736967-49736986 Msa0604280:intron 10.0%
!! TATATTAAATTTATTACTAC+AGG - chr4_2:49737074-49737093 Msa0604280:intron 10.0%
!! TATTAATAATAAATACAATG+AGG + chr4_2:49738494-49738513 None:intergenic 10.0%
!! TAATAAAAAACAAAAAAAGT+TGG - chr4_2:49738519-49738538 Msa0604280:intron 10.0%
!! AATAAAAAACAAAAAAAGTT+GGG - chr4_2:49738520-49738539 Msa0604280:intron 10.0%
!!! TTTATTTTAATTTGTTTGAT+TGG - chr4_2:49740289-49740308 Msa0604280:intron 10.0%
!! ACTTTATAATATATATTAGA+TGG + chr4_2:49741295-49741314 None:intergenic 10.0%
!! TAGTTTCTCATATATTATAA+AGG + chr4_2:49734375-49734394 None:intergenic 15.0%
!! TAAATGTTTACATTCAAATA+TGG + chr4_2:49734702-49734721 None:intergenic 15.0%
!!! ATTTAAAACTTGTTAATCAT+GGG - chr4_2:49734717-49734736 Msa0604280:intron 15.0%
!!! AAATATATTTTATTGCAAGT+GGG + chr4_2:49734758-49734777 None:intergenic 15.0%
!!! AAAATATATTTTATTGCAAG+TGG + chr4_2:49734759-49734778 None:intergenic 15.0%
!!! GATATCATTTTTTTTTTTGA+GGG + chr4_2:49735295-49735314 None:intergenic 15.0%
!!! TGATATCATTTTTTTTTTTG+AGG + chr4_2:49735296-49735315 None:intergenic 15.0%
!!! AACTTTTTAATAAAGACAAA+AGG - chr4_2:49735371-49735390 Msa0604280:intron 15.0%
!! CAAAAGATAATCTATTTAAA+TGG + chr4_2:49735655-49735674 None:intergenic 15.0%
!!! AATTTAAAATCAAACAATGA+AGG + chr4_2:49735805-49735824 None:intergenic 15.0%
!! GTATATTTAATGATAACTTA+AGG - chr4_2:49736000-49736019 Msa0604280:intron 15.0%
!! TTATATTGTAAAGTGATAAA+AGG + chr4_2:49736106-49736125 None:intergenic 15.0%
!!! GTTTTAAAAAAAACAAAAGA+GGG + chr4_2:49736178-49736197 None:intergenic 15.0%
!!! TGTTTTAAAAAAAACAAAAG+AGG + chr4_2:49736179-49736198 None:intergenic 15.0%
!!! AAAATCCTACAATTTATTTT+AGG + chr4_2:49736583-49736602 None:intergenic 15.0%
!! AATCAAAACAACAATATTAT+AGG + chr4_2:49736739-49736758 None:intergenic 15.0%
!!! GTTTTTAACATAATATTTTC+TGG - chr4_2:49737197-49737216 Msa0604280:intron 15.0%
!!! TTTTTATGAAGTAAAATTGT+TGG + chr4_2:49737246-49737265 None:intergenic 15.0%
!!! ATTTTACTTCATAAAAATAG+AGG - chr4_2:49737249-49737268 Msa0604280:intron 15.0%
!!! TTTTACTTCATAAAAATAGA+GGG - chr4_2:49737250-49737269 Msa0604280:intron 15.0%
!! ACAAAATTTAACAAATAGTT+TGG + chr4_2:49737371-49737390 None:intergenic 15.0%
!! CAAATATTCATAATATATGT+CGG - chr4_2:49737425-49737444 Msa0604280:intron 15.0%
!! ATGTATAGATAACTATAATT+TGG - chr4_2:49737591-49737610 Msa0604280:CDS 15.0%
!! AATTATGTTGTATTATGTAA+GGG + chr4_2:49738325-49738344 None:intergenic 15.0%
!! AAATTATGTTGTATTATGTA+AGG + chr4_2:49738326-49738345 None:intergenic 15.0%
!!! TTTTCTTTTTTAAACAATTG+TGG + chr4_2:49738662-49738681 None:intergenic 15.0%
!!! TTCTTTGAAAAATATATTTG+AGG + chr4_2:49738766-49738785 None:intergenic 15.0%
!! AATTCAAAATAAAAAGTTGA+AGG - chr4_2:49739146-49739165 Msa0604280:intron 15.0%
!! AAATAAACTACAAAATTAAC+CGG - chr4_2:49739507-49739526 Msa0604280:CDS 15.0%
!! CAAATGTATCAAAATATTAA+AGG - chr4_2:49740245-49740264 Msa0604280:intron 15.0%
!!! AAACATACATTTTGTATAAT+AGG + chr4_2:49741012-49741031 None:intergenic 15.0%
!!! TGGTTTTTTTTTTTTTTTAC+AGG - chr4_2:49741369-49741388 Msa0604280:intron 15.0%
!!! GGTTTTTTTTTTTTTTTACA+GGG - chr4_2:49741370-49741389 Msa0604280:intron 15.0%
!!! AAGATTAACAACTTTTATTT+AGG + chr4_2:49741610-49741629 None:intergenic 15.0%
!! ACTAACACAAAAAAGTAAAT+TGG - chr4_2:49733702-49733721 Msa0604280:three_prime_UTR 20.0%
!! CAAAATTGGTTAACATTATT+TGG + chr4_2:49734218-49734237 None:intergenic 20.0%
!!! AATAATGTTAACCAATTTTG+AGG - chr4_2:49734218-49734237 Msa0604280:CDS 20.0%
!!! TGATTTAGAAAAGCTTTTAT+TGG - chr4_2:49734251-49734270 Msa0604280:CDS 20.0%
!! AACTAGAAATTGAAATTAAC+TGG + chr4_2:49734625-49734644 None:intergenic 20.0%
!!! CATTTAAAACTTGTTAATCA+TGG - chr4_2:49734716-49734735 Msa0604280:intron 20.0%
!! GCATAATATCTTAATCTTAA+AGG + chr4_2:49734806-49734825 None:intergenic 20.0%
!!! TTTTTTTTTGTTGAAGAATC+CGG - chr4_2:49735010-49735029 Msa0604280:CDS 20.0%
!! TGATTTATGTATATCAAACT+TGG + chr4_2:49735436-49735455 None:intergenic 20.0%
!! AGATAATCTATTTAAATGGA+GGG + chr4_2:49735651-49735670 None:intergenic 20.0%
!! AAGATAATCTATTTAAATGG+AGG + chr4_2:49735652-49735671 None:intergenic 20.0%
!! AATTCCTATTTAATATAGGA+TGG + chr4_2:49735952-49735971 None:intergenic 20.0%
!!! TTATATTAGTTTCTGTAACT+AGG - chr4_2:49736131-49736150 Msa0604280:intron 20.0%
!!! ATCATTAATCAATTTGAAGT+GGG + chr4_2:49736313-49736332 None:intergenic 20.0%
!! TATCATTAATCAATTTGAAG+TGG + chr4_2:49736314-49736333 None:intergenic 20.0%
!!! TTGTTGGTAATAAAAAACAA+TGG - chr4_2:49736387-49736406 Msa0604280:intron 20.0%
!! AACAAATGACTAATCAAATT+TGG + chr4_2:49736538-49736557 None:intergenic 20.0%
!!! TTTGATTAGTCATTTGTTAT+CGG - chr4_2:49736540-49736559 Msa0604280:intron 20.0%
!!! TATGCTTACATTTTTTTTGA+AGG + chr4_2:49736632-49736651 None:intergenic 20.0%
!! TTAACACATGTAATATGAAT+TGG - chr4_2:49736944-49736963 Msa0604280:intron 20.0%
!!! ATTTCATTCCATTTTTTTTG+AGG - chr4_2:49736994-49737013 Msa0604280:intron 20.0%
!!! TTTCATTCCATTTTTTTTGA+GGG - chr4_2:49736995-49737014 Msa0604280:intron 20.0%
!!! TTAACATAATATTTTCTGGT+TGG - chr4_2:49737201-49737220 Msa0604280:intron 20.0%
!! TTTACTTCATAAAAATAGAG+GGG - chr4_2:49737251-49737270 Msa0604280:intron 20.0%
!! ATGGTAACATTCAAAATTTA+CGG - chr4_2:49737344-49737363 Msa0604280:CDS 20.0%
!! TGATTATGTGTAACTATATA+AGG + chr4_2:49737804-49737823 None:intergenic 20.0%
!!! TCCTTGTTTTTAAACAAATT+TGG - chr4_2:49737911-49737930 Msa0604280:CDS 20.0%
!!! TCCAAATTTGTTTAAAAACA+AGG + chr4_2:49737915-49737934 None:intergenic 20.0%
!! TAATTAATTACCATCCAAAT+TGG + chr4_2:49738095-49738114 None:intergenic 20.0%
!! ATTATGTTGTATTATGTAAG+GGG + chr4_2:49738324-49738343 None:intergenic 20.0%
!!! CTATTTTACTTAGATTTGTT+TGG + chr4_2:49738564-49738583 None:intergenic 20.0%
!! ATCTAAGTAAAATAGTATGT+AGG - chr4_2:49738569-49738588 Msa0604280:intron 20.0%
!! AAACTAATCCTAATAAAACT+AGG - chr4_2:49738613-49738632 Msa0604280:intron 20.0%
!!! CTATATATCCTAGTTTTATT+AGG + chr4_2:49738624-49738643 None:intergenic 20.0%
!! AAATACATATTGTCAACAAT+TGG + chr4_2:49738918-49738937 None:intergenic 20.0%
!!! ATTAACCATTTTATCATGTT+TGG + chr4_2:49739068-49739087 None:intergenic 20.0%
!! ACATGATAAAATGGTTAATT+TGG - chr4_2:49739069-49739088 Msa0604280:intron 20.0%
!! TTTGTAAAGCTAATTCAAAA+AGG + chr4_2:49739188-49739207 None:intergenic 20.0%
!! TTTACAAAAAAATGAAAGAC+TGG - chr4_2:49739200-49739219 Msa0604280:intron 20.0%
!! TTACAAAAAAATGAAAGACT+GGG - chr4_2:49739201-49739220 Msa0604280:intron 20.0%
!!! ATAAAGACACTTTTTTTTTG+TGG - chr4_2:49739457-49739476 Msa0604280:intron 20.0%
!!! TTTGTAGTTTATTTTCACTT+GGG + chr4_2:49739501-49739520 None:intergenic 20.0%
!!! TTTTGTAGTTTATTTTCACT+TGG + chr4_2:49739502-49739521 None:intergenic 20.0%
!!! ATCATTTGATTTGATTGTTT+TGG + chr4_2:49739603-49739622 None:intergenic 20.0%
!!! AATGGTTCATTTGATTTTAT+TGG - chr4_2:49740422-49740441 Msa0604280:intron 20.0%
!! AAAATCAAACTGATATATGA+AGG + chr4_2:49741702-49741721 None:intergenic 20.0%
! AAACCAAATATGGTATTCAT+TGG - chr4_2:49733736-49733755 Msa0604280:three_prime_UTR 25.0%
! CAACAAAATATGTACATACT+TGG + chr4_2:49733918-49733937 None:intergenic 25.0%
!! AAAAATCGTGAAACGATTTT+AGG + chr4_2:49734098-49734117 None:intergenic 25.0%
!!! GTTACATGATTTTAGAATCA+TGG + chr4_2:49734187-49734206 None:intergenic 25.0%
!! AAAAGTTTTTGCCTCAAAAT+TGG + chr4_2:49734232-49734251 None:intergenic 25.0%
!! TTAGAAAAGCTTTTATTGGT+TGG - chr4_2:49734255-49734274 Msa0604280:CDS 25.0%
! AATTCACGAGTTTAATTACA+AGG + chr4_2:49734316-49734335 None:intergenic 25.0%
! ACATACAAAATGTTAATGAG+AGG - chr4_2:49734336-49734355 Msa0604280:intron 25.0%
! CAAACATGTATATAGTTTGA+AGG - chr4_2:49734459-49734478 Msa0604280:intron 25.0%
! ATATCTTAATCTTAAAGGAG+AGG + chr4_2:49734801-49734820 None:intergenic 25.0%
!! TTTTAATAGCCATAGTATGT+AGG - chr4_2:49734930-49734949 Msa0604280:CDS 25.0%
!!! TCGTCTTTTAAGAAAATATC+CGG - chr4_2:49735218-49735237 Msa0604280:intron 25.0%
!! TTTAAAGAATTTTGCTCTAC+CGG + chr4_2:49735240-49735259 None:intergenic 25.0%
! TATATCAAACTTGTATAGCA+TGG + chr4_2:49735339-49735358 None:intergenic 25.0%
!!! ACTTTTTTCTCAATGACAAA+AGG - chr4_2:49735463-49735482 Msa0604280:intron 25.0%
! ACATGTTTAATCAAAACATC+TGG + chr4_2:49735725-49735744 None:intergenic 25.0%
! AATGTAGAAAAAATAAGGGA+TGG - chr4_2:49735874-49735893 Msa0604280:intron 25.0%
! TGGTGCCTATAATAATAAAT+AGG - chr4_2:49736028-49736047 Msa0604280:intron 25.0%
! TATTGTAAAGTGATAAAAGG+TGG + chr4_2:49736103-49736122 None:intergenic 25.0%
!! TCATTAATCAATTTGAAGTG+GGG + chr4_2:49736312-49736331 None:intergenic 25.0%
! AACAAAAAGAGAAACATTGA+AGG + chr4_2:49736372-49736391 None:intergenic 25.0%
!! TTCAATGTTTCTCTTTTTGT+TGG - chr4_2:49736371-49736390 Msa0604280:intron 25.0%
! GAGTACCTAAAATAAATTGT+AGG - chr4_2:49736575-49736594 Msa0604280:intron 25.0%
! ATGTAAGCATACACTATTAA+TGG - chr4_2:49736641-49736660 Msa0604280:intron 25.0%
! TCAAAATAACAAGATTGCAT+TGG - chr4_2:49736840-49736859 Msa0604280:intron 25.0%
! TGAAATTCCCTCAAAAAAAA+TGG + chr4_2:49737005-49737024 None:intergenic 25.0%
!!! GATCAAATTTTGACTTACTT+GGG + chr4_2:49737170-49737189 None:intergenic 25.0%
!!! TGATCAAATTTTGACTTACT+TGG + chr4_2:49737171-49737190 None:intergenic 25.0%
!! ATATATGTCGGTGAAATTTT+AGG - chr4_2:49737437-49737456 Msa0604280:intron 25.0%
!!! ATTCGTAACTCTTTTTCATT+GGG - chr4_2:49737469-49737488 Msa0604280:intron 25.0%
! TAGGCAAATGATATTTGAAA+TGG + chr4_2:49737558-49737577 None:intergenic 25.0%
! TGTGTATAAGATCAATTATC+AGG - chr4_2:49737851-49737870 Msa0604280:intron 25.0%
!! AATGCGATATGCATTAATTT+TGG - chr4_2:49738370-49738389 Msa0604280:intron 25.0%
!! ATGCGATATGCATTAATTTT+GGG - chr4_2:49738371-49738390 Msa0604280:intron 25.0%
! AAAAAACAAAAAAAGTTGGG+AGG - chr4_2:49738523-49738542 Msa0604280:intron 25.0%
!! TTACTTAGATTTGTTTGGTT+TGG + chr4_2:49738559-49738578 None:intergenic 25.0%
! AGAAAAACTTTACTTCTATC+CGG - chr4_2:49738676-49738695 Msa0604280:intron 25.0%
! GAAATCCAAACATGATAAAA+TGG - chr4_2:49739060-49739079 Msa0604280:intron 25.0%
!! TGATAAAATGGTTAATTTGG+AGG - chr4_2:49739072-49739091 Msa0604280:intron 25.0%
!! GACAAAAAACATGTTTTCTT+CGG + chr4_2:49739386-49739405 None:intergenic 25.0%
!!! TTTTTTTGTGGTACAAAAAG+AGG - chr4_2:49739469-49739488 Msa0604280:intron 25.0%
!!! TTTTTTGTGGTACAAAAAGA+GGG - chr4_2:49739470-49739489 Msa0604280:intron 25.0%
! TTAGTCATAAGAATACTACT+TGG + chr4_2:49740077-49740096 None:intergenic 25.0%
!! TTAAATGTGTGTATGGATTT+TGG + chr4_2:49740197-49740216 None:intergenic 25.0%
! TTTAAACACTTCATAGGTTA+AGG - chr4_2:49740212-49740231 Msa0604280:intron 25.0%
!! TTGTTTGATTGGTTAATTTC+AGG - chr4_2:49740300-49740319 Msa0604280:intron 25.0%
!!! ATTGGTTAATTTCAGGTTTA+TGG - chr4_2:49740307-49740326 Msa0604280:intron 25.0%
!! TTACATGAAGTATTAGTCAT+AGG + chr4_2:49740524-49740543 None:intergenic 25.0%
! TATGACTAATACTTCATGTA+AGG - chr4_2:49740523-49740542 Msa0604280:intron 25.0%
! TTAAAGGAACTAATTAACAC+AGG + chr4_2:49740573-49740592 None:intergenic 25.0%
!!! AGATCTTTTGTTATACACTA+AGG - chr4_2:49740890-49740909 Msa0604280:CDS 25.0%
! CATATTCTTTCAAATTAGTC+AGG + chr4_2:49741090-49741109 None:intergenic 25.0%
! AGAATATGTATGATATGTAC+AGG - chr4_2:49741102-49741121 Msa0604280:intron 25.0%
! GAAAGATTATTACTTGATTG+CGG + chr4_2:49741226-49741245 None:intergenic 25.0%
!! AGTTATGAATTTTCATTTCG+TGG - chr4_2:49741349-49741368 Msa0604280:intron 25.0%
!!! TTTTTTTTTTTTTACAGGGC+CGG - chr4_2:49741374-49741393 Msa0604280:intron 25.0%
! AACTTTGTAGATTACAATGA+TGG - chr4_2:49741479-49741498 Msa0604280:intron 25.0%
!!! ATTACAATGATGGATTGAAA+AGG - chr4_2:49741489-49741508 Msa0604280:intron 25.0%
!!! TTTTATTTAGGTGCTGAATT+TGG + chr4_2:49741598-49741617 None:intergenic 25.0%
! CCAACTAATATATATGATCA+TGG + chr4_2:49741678-49741697 None:intergenic 25.0%
! CCATGATCATATATATTAGT+TGG - chr4_2:49741675-49741694 Msa0604280:CDS 25.0%
TAGCCAATGAATACCATATT+TGG + chr4_2:49733742-49733761 None:intergenic 30.0%
! ATTCATTGGCTATTTCAATG+TGG - chr4_2:49733750-49733769 Msa0604280:three_prime_UTR 30.0%
CTGTATAGAGTAATTCTTCT+TGG - chr4_2:49733967-49733986 Msa0604280:CDS 30.0%
! CACTAGTTTAAGCACTATTA+AGG + chr4_2:49734003-49734022 None:intergenic 30.0%
!!! GTGACATGATTTTAGAATCA+TGG + chr4_2:49734157-49734176 None:intergenic 30.0%
AAAAAAACCTGGATGTTGAT+GGG + chr4_2:49734532-49734551 None:intergenic 30.0%
AAAAAAAACCTGGATGTTGA+TGG + chr4_2:49734533-49734552 None:intergenic 30.0%
CTAACATACAGAGAAAACAA+AGG - chr4_2:49734581-49734600 Msa0604280:intron 30.0%
!! TATTTTATTGCAAGTGGGTT+AGG + chr4_2:49734753-49734772 None:intergenic 30.0%
!!! CATTTACGAACTTTTCAAGT+AGG + chr4_2:49734852-49734871 None:intergenic 30.0%
ACAAACAAACCTACATACTA+TGG + chr4_2:49734942-49734961 None:intergenic 30.0%
!!! GTTTTTTTTTTACCGGTAGT+AGG - chr4_2:49734968-49734987 Msa0604280:CDS 30.0%
TATTAAAGTTAAGCACTCCA+CGG - chr4_2:49735399-49735418 Msa0604280:intron 30.0%
! ACTTAGCATATTTTATGCCA+TGG + chr4_2:49735519-49735538 None:intergenic 30.0%
CATAAAATATGCTAAGTGTG+AGG - chr4_2:49735522-49735541 Msa0604280:intron 30.0%
TTAATCAAAACATCTGGCAT+AGG + chr4_2:49735719-49735738 None:intergenic 30.0%
AGCTCCATCCTATATTAAAT+AGG - chr4_2:49735945-49735964 Msa0604280:intron 30.0%
ATTATCCTTAGTCGTGAAAT+AGG - chr4_2:49735972-49735991 Msa0604280:intron 30.0%
AATGATAACTTAAGGGAAGT+TGG - chr4_2:49736008-49736027 Msa0604280:intron 30.0%
!! GTCATTCAACTCTTTAAAGT+CGG + chr4_2:49736289-49736308 None:intergenic 30.0%
! CATTAATCAATTTGAAGTGG+GGG + chr4_2:49736311-49736330 None:intergenic 30.0%
TTATTACTACAGGAAGATGT+TGG - chr4_2:49737084-49737103 Msa0604280:intron 30.0%
TATTACTACAGGAAGATGTT+GGG - chr4_2:49737085-49737104 Msa0604280:intron 30.0%
GATTATGAAGGATCAGAATA+TGG - chr4_2:49737107-49737126 Msa0604280:intron 30.0%
CAATGAAAAAGAGTTACGAA+TGG + chr4_2:49737470-49737489 None:intergenic 30.0%
!! CATTCGTAACTCTTTTTCAT+TGG - chr4_2:49737468-49737487 Msa0604280:intron 30.0%
TTAAAAACAAGGACTAGAGA+CGG + chr4_2:49737904-49737923 None:intergenic 30.0%
TTAAAGTTACATAAGTGTGC+AGG - chr4_2:49737963-49737982 Msa0604280:intron 30.0%
! GCATAACATTGGTAACAAAT+TGG - chr4_2:49738007-49738026 Msa0604280:intron 30.0%
! TGCGATATGCATTAATTTTG+GGG - chr4_2:49738372-49738391 Msa0604280:intron 30.0%
AACATTGTATTACGATGACT+TGG + chr4_2:49738422-49738441 None:intergenic 30.0%
TAAATACAATGAGGTGCATA+CGG + chr4_2:49738485-49738504 None:intergenic 30.0%
!! TAGATTTGTTTGGTTTGGTT+TGG + chr4_2:49738554-49738573 None:intergenic 30.0%
AAATGCAATCTTATGACTTC+CGG + chr4_2:49738698-49738717 None:intergenic 30.0%
ACTGTGAGAAAAGAATTTCA+AGG - chr4_2:49739112-49739131 Msa0604280:intron 30.0%
CTGTGAGAAAAGAATTTCAA+GGG - chr4_2:49739113-49739132 Msa0604280:intron 30.0%
TTGAAGGATTGTTAGTGTTT+GGG - chr4_2:49739162-49739181 Msa0604280:intron 30.0%
AAATGATGCATTGGGATTAA+AGG - chr4_2:49739412-49739431 Msa0604280:intron 30.0%
TATCAAGAAACTACTGCAAA+TGG + chr4_2:49739707-49739726 None:intergenic 30.0%
! CTGACAAGTTCAAGTTTAAT+GGG + chr4_2:49739748-49739767 None:intergenic 30.0%
! TCTGACAAGTTCAAGTTTAA+TGG + chr4_2:49739749-49739768 None:intergenic 30.0%
!! GCATTTTGATCATCTTATAG+TGG + chr4_2:49739804-49739823 None:intergenic 30.0%
! TTTTTGATGCAGATGGTAAA+TGG + chr4_2:49739919-49739938 None:intergenic 30.0%
!!! GGTATTTTTTTTGATGCAGA+TGG + chr4_2:49739926-49739945 None:intergenic 30.0%
AAAAAAAATACCACCACAAC+TGG - chr4_2:49739934-49739953 Msa0604280:intron 30.0%
! GAAGTGTTTAAATGTGTGTA+TGG + chr4_2:49740204-49740223 None:intergenic 30.0%
CACACATTTAAACACTTCAT+AGG - chr4_2:49740206-49740225 Msa0604280:intron 30.0%
ATTTAGTGAACTTAGGCAAT+CGG + chr4_2:49740385-49740404 None:intergenic 30.0%
GATTGCAATTTAGTGAACTT+AGG + chr4_2:49740392-49740411 None:intergenic 30.0%
ATAGGATCTGTTAAGTAACT+TGG + chr4_2:49740506-49740525 None:intergenic 30.0%
ACAATTGCAGTTGAACAAAA+TGG - chr4_2:49740645-49740664 Msa0604280:intron 30.0%
ATATATAGGCTGCTTCAATT+TGG - chr4_2:49740841-49740860 Msa0604280:intron 30.0%
!! ATTTTTCGCATGGTCATTTT+TGG - chr4_2:49741412-49741431 Msa0604280:intron 30.0%
!! CATTTTTGGACTGTAATGAA+TGG - chr4_2:49741426-49741445 Msa0604280:intron 30.0%
! ATAGTTTGAAGGTGCAGTAA+AGG - chr4_2:49734470-49734489 Msa0604280:CDS 35.0%
AATCTTAAAGGAGAGGAGTT+AGG + chr4_2:49734794-49734813 None:intergenic 35.0%
!!! TTTTTTTACCGGTAGTAGGT+AGG - chr4_2:49734972-49734991 Msa0604280:CDS 35.0%
AAAAGAAACCTACCTACTAC+CGG + chr4_2:49734983-49735002 None:intergenic 35.0%
AAAGTTAATAAGCACCACCA+TGG - chr4_2:49735499-49735518 Msa0604280:intron 35.0%
! TAGCATATTTTATGCCATGG+TGG + chr4_2:49735516-49735535 None:intergenic 35.0%
CTATTTAAATGGAGGGTCTA+TGG + chr4_2:49735644-49735663 None:intergenic 35.0%
TAGGTTGTTCGTATGGAAAT+TGG + chr4_2:49735700-49735719 None:intergenic 35.0%
TTTCTCCTATTTCACGACTA+AGG + chr4_2:49735980-49735999 None:intergenic 35.0%
TTTCTGTAACTAGGTTTCCA+GGG - chr4_2:49736140-49736159 Msa0604280:intron 35.0%
! ATCAACGAATTGTTGGCTAA+TGG - chr4_2:49737325-49737344 Msa0604280:CDS 35.0%
!! ATACTCGTCTTCTAAGACTT+GGG + chr4_2:49737501-49737520 None:intergenic 35.0%
ATACATTGAATCGCTATGCT+AGG + chr4_2:49737577-49737596 None:intergenic 35.0%
GATCTTTGCTTCAAGGAATT+CGG - chr4_2:49737648-49737667 Msa0604280:CDS 35.0%
!! TAGTAATGGTGGATATGCAA+TGG - chr4_2:49737716-49737735 Msa0604280:CDS 35.0%
!! AGTAATGGTGGATATGCAAT+GGG - chr4_2:49737717-49737736 Msa0604280:CDS 35.0%
AATTACCACCCAAACATGTT+GGG + chr4_2:49737770-49737789 None:intergenic 35.0%
! ACAAATTGGTTCATACCACT+TGG - chr4_2:49738021-49738040 Msa0604280:intron 35.0%
AGATCACTTGACTTCCAATT+TGG - chr4_2:49738078-49738097 Msa0604280:CDS 35.0%
!! TCTAATACACCGATTCCATT+TGG - chr4_2:49738199-49738218 Msa0604280:intron 35.0%
AGAGTGTATCCAAATGGAAT+CGG + chr4_2:49738211-49738230 None:intergenic 35.0%
TCCATTTGGATACACTCTTT+CGG - chr4_2:49738213-49738232 Msa0604280:intron 35.0%
TTCAGAGGTGAAGAAAATGA+TGG + chr4_2:49738250-49738269 None:intergenic 35.0%
! ATTTTCTTCACCTCTGAACA+GGG - chr4_2:49738252-49738271 Msa0604280:CDS 35.0%
! GCGATATGCATTAATTTTGG+GGG - chr4_2:49738373-49738392 Msa0604280:intron 35.0%
GTTGAAGGATTGTTAGTGTT+TGG - chr4_2:49739161-49739180 Msa0604280:intron 35.0%
AACAAGGCTGCATTTAAAGA+GGG + chr4_2:49739254-49739273 None:intergenic 35.0%
TAACAAGGCTGCATTTAAAG+AGG + chr4_2:49739255-49739274 None:intergenic 35.0%
!!! CAGATTTTTTTTTAGGGTGG+GGG + chr4_2:49739284-49739303 None:intergenic 35.0%
!!! CCAGATTTTTTTTTAGGGTG+GGG + chr4_2:49739285-49739304 None:intergenic 35.0%
!!! CCCAGATTTTTTTTTAGGGT+GGG + chr4_2:49739286-49739305 None:intergenic 35.0%
CCCACCCTAAAAAAAAATCT+GGG - chr4_2:49739283-49739302 Msa0604280:intron 35.0%
TCCATTCGAAATGATGCATT+GGG - chr4_2:49739404-49739423 Msa0604280:intron 35.0%
!!! AGGAGAGTTTTAGAAATTGC+TGG - chr4_2:49739432-49739451 Msa0604280:intron 35.0%
AAAATTAACCGGACATTCCT+AGG - chr4_2:49739518-49739537 Msa0604280:CDS 35.0%
! CATGTTTGATGATATGTCCA+AGG + chr4_2:49739552-49739571 None:intergenic 35.0%
!! AAGGAAATAGAAGTGCCAAT+TGG + chr4_2:49739651-49739670 None:intergenic 35.0%
ATGTGCTAATCAACCATACT+TGG + chr4_2:49739678-49739697 None:intergenic 35.0%
GTTGTATTTGGGTATAGAGA+TGG + chr4_2:49739837-49739856 None:intergenic 35.0%
AAAGGCTACACTAAGAAGAT+TGG + chr4_2:49739893-49739912 None:intergenic 35.0%
!! GCAGATGGTAAATGGATTAA+AGG + chr4_2:49739911-49739930 None:intergenic 35.0%
GTGAAGGACACTATTTACAT+AGG + chr4_2:49740031-49740050 None:intergenic 35.0%
TTTATGGAACCTCTAACAAC+CGG - chr4_2:49740323-49740342 Msa0604280:intron 35.0%
!!! CATGCTTTTAGGATAATCTC+CGG + chr4_2:49740345-49740364 None:intergenic 35.0%
TTGCAATCAAAGCTAGTGAA+TGG - chr4_2:49740404-49740423 Msa0604280:intron 35.0%
ATTAACCATTTACCTCCACA+AGG - chr4_2:49740470-49740489 Msa0604280:intron 35.0%
GAACAAAATGGAAAACCTCT+AGG - chr4_2:49740657-49740676 Msa0604280:intron 35.0%
TGGATATACGTTTATCCAAG+AGG - chr4_2:49740861-49740880 Msa0604280:intron 35.0%
! TTTTCAGTAATGTAGACCAC+AGG + chr4_2:49740930-49740949 None:intergenic 35.0%
GTGGTCTACATTACTGAAAA+TGG - chr4_2:49740930-49740949 Msa0604280:intron 35.0%
TTCTTGAATGCCATCATAAC+TGG - chr4_2:49740980-49740999 Msa0604280:intron 35.0%
TAGGATTGTTCCAGTTATGA+TGG + chr4_2:49740993-49741012 None:intergenic 35.0%
! TTCTGTAGAAGAAGCTCTTT+TGG - chr4_2:49741152-49741171 Msa0604280:intron 35.0%
! ATGTAAAGGGATTTTTCGCA+TGG - chr4_2:49741402-49741421 Msa0604280:intron 35.0%
! AGTCCTATATTGTTTGAAGC+TGG - chr4_2:49741561-49741580 Msa0604280:CDS 35.0%
ATATATATGATCATGGCTGC+TGG + chr4_2:49741671-49741690 None:intergenic 35.0%
GTCCTCCTTCATGTATAAAG+AGG - chr4_2:49733674-49733693 Msa0604280:three_prime_UTR 40.0%
TGCCTCTTTATACATGAAGG+AGG + chr4_2:49733679-49733698 None:intergenic 40.0%
GTCTGCCTCTTTATACATGA+AGG + chr4_2:49733682-49733701 None:intergenic 40.0%
CAAGCAGCACAAACCAAATA+TGG - chr4_2:49733726-49733745 Msa0604280:three_prime_UTR 40.0%
AACAACAAAGAGAGCAAGCA+TGG + chr4_2:49733776-49733795 None:intergenic 40.0%
GTCACACTTACTTTCGCAAA+CGG - chr4_2:49733802-49733821 Msa0604280:three_prime_UTR 40.0%
GTTTCAACTCCTCTTGACAT+CGG - chr4_2:49733832-49733851 Msa0604280:three_prime_UTR 40.0%
CTCTATACAGTTGCGTGAAT+AGG + chr4_2:49733957-49733976 None:intergenic 40.0%
ATGGCACGATGATAGAATGT+GGG + chr4_2:49734138-49734157 None:intergenic 40.0%
! TTTGAAGGTGCAGTAAAGGA+AGG - chr4_2:49734474-49734493 Msa0604280:CDS 40.0%
TTGTTGAAGAATCCGGTAGT+AGG - chr4_2:49735017-49735036 Msa0604280:CDS 40.0%
TATACCAACCTACCTACTAC+CGG + chr4_2:49735032-49735051 None:intergenic 40.0%
! ACTTGGCATATTTTATGCCG+TGG + chr4_2:49735419-49735438 None:intergenic 40.0%
TGACCAATACCATCGCTATA+AGG - chr4_2:49735918-49735937 Msa0604280:intron 40.0%
GTACCTTATAGCGATGGTAT+TGG + chr4_2:49735924-49735943 None:intergenic 40.0%
GTTTCTGTAACTAGGTTTCC+AGG - chr4_2:49736139-49736158 Msa0604280:intron 40.0%
TTGTTATCGGATCACTCTCT+AGG - chr4_2:49736553-49736572 Msa0604280:intron 40.0%
GGAAGATGTTGGGATTATGA+AGG - chr4_2:49737095-49737114 Msa0604280:intron 40.0%
CCAAGCGATTGAGAATCTAT+AGG + chr4_2:49737136-49737155 None:intergenic 40.0%
CCTATAGATTCTCAATCGCT+TGG - chr4_2:49737133-49737152 Msa0604280:intron 40.0%
!! GACTTACTTGGGAGTATTCT+TGG + chr4_2:49737159-49737178 None:intergenic 40.0%
AAAATAGAGGGGAAGTTGAG+TGG - chr4_2:49737262-49737281 Msa0604280:intron 40.0%
! AGTGGAGGCATAAATCATGA+AGG - chr4_2:49737280-49737299 Msa0604280:intron 40.0%
CAACCTCATCAACGAATTGT+TGG - chr4_2:49737318-49737337 Msa0604280:CDS 40.0%
TAGCCAACAATTCGTTGATG+AGG + chr4_2:49737324-49737343 None:intergenic 40.0%
!! TACTCGTCTTCTAAGACTTG+GGG + chr4_2:49737500-49737519 None:intergenic 40.0%
!! CATACTCGTCTTCTAAGACT+TGG + chr4_2:49737502-49737521 None:intergenic 40.0%
CAAGTCTTAGAAGACGAGTA+TGG - chr4_2:49737500-49737519 Msa0604280:intron 40.0%
CTATGCTGATCTTTGCTTCA+AGG - chr4_2:49737641-49737660 Msa0604280:CDS 40.0%
GAGCCATGGTTGTTTAGTAA+TGG - chr4_2:49737702-49737721 Msa0604280:CDS 40.0%
CCACCATTACTAAACAACCA+TGG + chr4_2:49737708-49737727 None:intergenic 40.0%
CCATGGTTGTTTAGTAATGG+TGG - chr4_2:49737705-49737724 Msa0604280:CDS 40.0%
ATGTTCTAGCCCAACATGTT+TGG - chr4_2:49737758-49737777 Msa0604280:CDS 40.0%
TGTTCTAGCCCAACATGTTT+GGG - chr4_2:49737759-49737778 Msa0604280:CDS 40.0%
GAATTACCACCCAAACATGT+TGG + chr4_2:49737771-49737790 None:intergenic 40.0%
ACATGTTTGGGTGGTAATTC+TGG - chr4_2:49737771-49737790 Msa0604280:CDS 40.0%
AAGTGTGCAGGCATATCAAA+AGG - chr4_2:49737975-49737994 Msa0604280:intron 40.0%
AGTGTGCAGGCATATCAAAA+GGG - chr4_2:49737976-49737995 Msa0604280:intron 40.0%
GCATATCAAAAGGGCAAGAT+AGG - chr4_2:49737985-49738004 Msa0604280:intron 40.0%
GGTATGCTATTGTTTCCAAG+TGG + chr4_2:49738039-49738058 None:intergenic 40.0%
CAATAGCATACCCGATCAAA+AGG - chr4_2:49738047-49738066 Msa0604280:intron 40.0%
TTGGAAGTCAAGTGATCTCT+TGG + chr4_2:49738076-49738095 None:intergenic 40.0%
CACTTGACTTCCAATTTGGA+TGG - chr4_2:49738082-49738101 Msa0604280:CDS 40.0%
! GTGTATTAGAGATGGTAGTG+GGG + chr4_2:49738189-49738208 None:intergenic 40.0%
GGTGTATTAGAGATGGTAGT+GGG + chr4_2:49738190-49738209 None:intergenic 40.0%
TGGAATCGGTGTATTAGAGA+TGG + chr4_2:49738197-49738216 None:intergenic 40.0%
! TCCGAAAGAGTGTATCCAAA+TGG + chr4_2:49738217-49738236 None:intergenic 40.0%
TTTGGATACACTCTTTCGGA+CGG - chr4_2:49738217-49738236 Msa0604280:intron 40.0%
! CATTTTCTTCACCTCTGAAC+AGG - chr4_2:49738251-49738270 Msa0604280:CDS 40.0%
! CTTTTCTCAACCTTGACCTA+GGG + chr4_2:49738299-49738318 None:intergenic 40.0%
! TCTTTTCTCAACCTTGACCT+AGG + chr4_2:49738300-49738319 None:intergenic 40.0%
!! GACTTGGTGTCAAGATAGTT+TGG + chr4_2:49738406-49738425 None:intergenic 40.0%
CATAGCAAAACGTGCAAAGA+AGG + chr4_2:49739227-49739246 None:intergenic 40.0%
CCCCACCCTAAAAAAAAATC+TGG - chr4_2:49739282-49739301 Msa0604280:intron 40.0%
!!! GCCCAGATTTTTTTTTAGGG+TGG + chr4_2:49739287-49739306 None:intergenic 40.0%
!!! GGGGCCCAGATTTTTTTTTA+GGG + chr4_2:49739290-49739309 None:intergenic 40.0%
GTCCATTCGAAATGATGCAT+TGG - chr4_2:49739403-49739422 Msa0604280:intron 40.0%
TCCCAATGCATCATTTCGAA+TGG + chr4_2:49739408-49739427 None:intergenic 40.0%
!!! CATGAACTTTTGAAGCTCAG+TGG - chr4_2:49739568-49739587 Msa0604280:intron 40.0%
GCTAAAACTAGCAAGCCAAT+TGG - chr4_2:49739633-49739652 Msa0604280:intron 40.0%
ATTTGGGTATAGAGATGGCT+TGG + chr4_2:49739832-49739851 None:intergenic 40.0%
TGTCTTACATGCTGAGATGT+GGG + chr4_2:49739861-49739880 None:intergenic 40.0%
ATGTCTTACATGCTGAGATG+TGG + chr4_2:49739862-49739881 None:intergenic 40.0%
AACTTCATCCTACCTTCTTG+AGG - chr4_2:49739995-49740014 Msa0604280:intron 40.0%
!! ACTATGGCTTGCATGCTTTT+AGG + chr4_2:49740356-49740375 None:intergenic 40.0%
!! AGGCAATCGGTTTTTCACTA+TGG + chr4_2:49740372-49740391 None:intergenic 40.0%
GGATCTGTTAAGTAACTTGG+AGG + chr4_2:49740503-49740522 None:intergenic 40.0%
AGTTACTAGAGACTTACCCT+TGG + chr4_2:49740684-49740703 None:intergenic 40.0%
AAAAGATCTCGAAGTCCTCT+TGG + chr4_2:49740879-49740898 None:intergenic 40.0%
GGAGAAATACAACAATCCTG+TGG - chr4_2:49740911-49740930 Msa0604280:CDS 40.0%
AAATCCCTTTACATTCGAGC+CGG + chr4_2:49741396-49741415 None:intergenic 40.0%
! GACTGTAATGAATGGTTTGC+AGG - chr4_2:49741434-49741453 Msa0604280:intron 40.0%
ACCATTGAGCAGAAAGCTTT+GGG + chr4_2:49741517-49741536 None:intergenic 40.0%
TACCATTGAGCAGAAAGCTT+TGG + chr4_2:49741518-49741537 None:intergenic 40.0%
GCACCAGCTTCAAACAATAT+AGG + chr4_2:49741567-49741586 None:intergenic 40.0%
TTTCCACGTGGCTTCATCTT+TGG - chr4_2:49733874-49733893 Msa0604280:CDS 45.0%
TTCCACGTGGCTTCATCTTT+GGG - chr4_2:49733875-49733894 Msa0604280:CDS 45.0%
CATGGCACGATGATAGAATG+TGG + chr4_2:49734139-49734158 None:intergenic 45.0%
TGGTAGAGGACCAAGTATCT+GGG - chr4_2:49734497-49734516 Msa0604280:CDS 45.0%
GAGAGGAGTTAGGTCTCAAT+AGG + chr4_2:49734784-49734803 None:intergenic 45.0%
TGAAGAATCCGGTAGTAGGT+AGG - chr4_2:49735021-49735040 Msa0604280:CDS 45.0%
TCTGGCATAGGTTGTTCGTA+TGG + chr4_2:49735707-49735726 None:intergenic 45.0%
GAGCTAGTACCTTATAGCGA+TGG + chr4_2:49735930-49735949 None:intergenic 45.0%
GTCTTAGAAGACGAGTATGG+TGG - chr4_2:49737503-49737522 Msa0604280:intron 45.0%
TCGGAGATAGAGTGAAGTAC+TGG - chr4_2:49737667-49737686 Msa0604280:CDS 45.0%
! CGGAGATAGAGTGAAGTACT+GGG - chr4_2:49737668-49737687 Msa0604280:CDS 45.0%
GGGTTACATTTAACGAGCCA+TGG - chr4_2:49737688-49737707 Msa0604280:CDS 45.0%
GGGCAAGATAGGCATAACAT+TGG - chr4_2:49737996-49738015 Msa0604280:intron 45.0%
! TCTCTTGGCAGCCTTTTGAT+CGG + chr4_2:49738061-49738080 None:intergenic 45.0%
CGGTGTATTAGAGATGGTAG+TGG + chr4_2:49738191-49738210 None:intergenic 45.0%
! GCATACGGTATGCGATGTTT+TGG + chr4_2:49738470-49738489 None:intergenic 45.0%
!!! GGGGGCCCAGATTTTTTTTT+AGG + chr4_2:49739291-49739310 None:intergenic 45.0%
! GATCCTTTGTTTCAGAGGCA+GGG + chr4_2:49739364-49739383 None:intergenic 45.0%
! CTTCGGATCCTTTGTTTCAG+AGG + chr4_2:49739369-49739388 None:intergenic 45.0%
!!! AACTTTTGAAGCTCAGTGGC+TGG - chr4_2:49739572-49739591 Msa0604280:intron 45.0%
TATTTCCTTCGCGCCAAGTA+TGG - chr4_2:49739662-49739681 Msa0604280:intron 45.0%
GAGATGTGGGGGTTGTATTT+GGG + chr4_2:49739848-49739867 None:intergenic 45.0%
TGAGATGTGGGGGTTGTATT+TGG + chr4_2:49739849-49739868 None:intergenic 45.0%
TCTTACATGCTGAGATGTGG+GGG + chr4_2:49739859-49739878 None:intergenic 45.0%
GTCTTACATGCTGAGATGTG+GGG + chr4_2:49739860-49739879 None:intergenic 45.0%
ATGGGTCGTATTTCCAGTTG+TGG + chr4_2:49739950-49739969 None:intergenic 45.0%
AATACGACCCATATCCTTGC+AGG - chr4_2:49739958-49739977 Msa0604280:intron 45.0%
AGAAACGACCTCAAGAAGGT+AGG + chr4_2:49740006-49740025 None:intergenic 45.0%
GGATAGAAACGACCTCAAGA+AGG + chr4_2:49740010-49740029 None:intergenic 45.0%
! TCATAGAGGGTCTAAGGTGA+AGG + chr4_2:49740047-49740066 None:intergenic 45.0%
TCACCTTAGACCCTCTATGA+AGG - chr4_2:49740047-49740066 Msa0604280:intron 45.0%
! ACTTGGAGCACCTTCATAGA+GGG + chr4_2:49740060-49740079 None:intergenic 45.0%
! TACTTGGAGCACCTTCATAG+AGG + chr4_2:49740061-49740080 None:intergenic 45.0%
TTCATAGGTTAAGGTGCGCA+AGG - chr4_2:49740221-49740240 Msa0604280:intron 45.0%
GGATAATCTCCGGTTGTTAG+AGG + chr4_2:49740335-49740354 None:intergenic 45.0%
CATTGCCTTGTGGAGGTAAA+TGG + chr4_2:49740478-49740497 None:intergenic 45.0%
ATGGAAAACCTCTAGGTCCA+AGG - chr4_2:49740664-49740683 Msa0604280:intron 45.0%
TGGAAAACCTCTAGGTCCAA+GGG - chr4_2:49740665-49740684 Msa0604280:intron 45.0%
! GCAGGCTTTACTGTTCGTTT+TGG - chr4_2:49741452-49741471 Msa0604280:intron 45.0%
ACCCAAAGCTTTCTGCTCAA+TGG - chr4_2:49741513-49741532 Msa0604280:CDS 45.0%
!!! AAAATTCTTTAAAAATAAAT+TGG - chr4_2:49735247-49735266 Msa0604280:intron 5.0%
!! ACTTTATATTATATATAATT+TGG + chr4_2:49736504-49736523 None:intergenic 5.0%
!! TTAATTTATCATAATATTAA+AGG + chr4_2:49740589-49740608 None:intergenic 5.0%
!! TTTATAATAATTGTATATAT+AGG - chr4_2:49740827-49740846 Msa0604280:intron 5.0%
TTGACATCGGCAACCTGAGT+CGG - chr4_2:49733845-49733864 Msa0604280:exon 50.0%
GTGGAAAACTACTCCGACTC+AGG + chr4_2:49733861-49733880 None:intergenic 50.0%
! AGTCGGAGTAGTTTTCCACG+TGG - chr4_2:49733862-49733881 Msa0604280:CDS 50.0%
TCCACGTGGCTTCATCTTTG+GGG - chr4_2:49733876-49733895 Msa0604280:CDS 50.0%
! GAAGGTGCAGTAAAGGAAGG+TGG - chr4_2:49734477-49734496 Msa0604280:CDS 50.0%
GCAGTAAAGGAAGGTGGTAG+AGG - chr4_2:49734483-49734502 Msa0604280:CDS 50.0%
GTGGTAGAGGACCAAGTATC+TGG - chr4_2:49734496-49734515 Msa0604280:CDS 50.0%
!! GTGAAGGTGTCCCAGATACT+TGG + chr4_2:49734510-49734529 None:intergenic 50.0%
ACCTTCACCCATCAACATCC+AGG - chr4_2:49734522-49734541 Msa0604280:CDS 50.0%
! ACCTGGATGTTGATGGGTGA+AGG + chr4_2:49734526-49734545 None:intergenic 50.0%
GAATCCGGTAGTAGGTAGGT+TGG - chr4_2:49735025-49735044 Msa0604280:CDS 50.0%
ATAGAGGGGAAGTTGAGTGG+AGG - chr4_2:49737265-49737284 Msa0604280:intron 50.0%
TCTAGCCCAACATGTTTGGG+TGG - chr4_2:49737762-49737781 Msa0604280:CDS 50.0%
! CTCTTGGCAGCCTTTTGATC+GGG + chr4_2:49738060-49738079 None:intergenic 50.0%
! GTAGTGGGGTGTAGCTTTCA+TGG + chr4_2:49738175-49738194 None:intergenic 50.0%
! GGGCCGTTCCTAGCATTTTA+CGG - chr4_2:49738272-49738291 Msa0604280:CDS 50.0%
! GGCCGTTCCTAGCATTTTAC+GGG - chr4_2:49738273-49738292 Msa0604280:CDS 50.0%
GGCCCGTAAAATGCTAGGAA+CGG + chr4_2:49738278-49738297 None:intergenic 50.0%
! ATTTTACGGGCCCTAGGTCA+AGG - chr4_2:49738286-49738305 Msa0604280:CDS 50.0%
! GGGTGGGGGTGTATATAACA+AGG + chr4_2:49739270-49739289 None:intergenic 50.0%
AAAAAAATCTGGGCCCCCCA+CGG - chr4_2:49739293-49739312 Msa0604280:intron 50.0%
! GGATCCTTTGTTTCAGAGGC+AGG + chr4_2:49739365-49739384 None:intergenic 50.0%
CTTGCATGCCTAGGAATGTC+CGG + chr4_2:49739529-49739548 None:intergenic 50.0%
ATTCCTAGGCATGCAAGCCT+TGG - chr4_2:49739532-49739551 Msa0604280:intron 50.0%
ATCAACCATACTTGGCGCGA+AGG + chr4_2:49739670-49739689 None:intergenic 50.0%
! GGGTATAGAGATGGCTTGGA+GGG + chr4_2:49739828-49739847 None:intergenic 50.0%
! TGGGTATAGAGATGGCTTGG+AGG + chr4_2:49739829-49739848 None:intergenic 50.0%
GGTCGTATTTCCAGTTGTGG+TGG + chr4_2:49739947-49739966 None:intergenic 50.0%
GACAGTGCCTGCAAGGATAT+GGG + chr4_2:49739968-49739987 None:intergenic 50.0%
GCACCTTCATAGAGGGTCTA+AGG + chr4_2:49740053-49740072 None:intergenic 50.0%
AGACTTACCCTTGGACCTAG+AGG + chr4_2:49740675-49740694 None:intergenic 50.0%
TGATCATGGCTGCTGGTACA+AGG + chr4_2:49741664-49741683 None:intergenic 50.0%
! CTCAGGTTGCCGATGTCAAG+AGG + chr4_2:49733844-49733863 None:intergenic 55.0%
! ACGTGGCTTCATCTTTGGGG+CGG - chr4_2:49733879-49733898 Msa0604280:CDS 55.0%
GCAATGGGAACAACAGCACC+AGG - chr4_2:49737732-49737751 Msa0604280:CDS 55.0%
! GTTGGGCTAGAACATCGACC+TGG + chr4_2:49737753-49737772 None:intergenic 55.0%
CCTAGGGCCCGTAAAATGCT+AGG + chr4_2:49738283-49738302 None:intergenic 55.0%
! CCTAGCATTTTACGGGCCCT+AGG - chr4_2:49738280-49738299 Msa0604280:CDS 55.0%
AAAAAATCTGGGCCCCCCAC+GGG - chr4_2:49739294-49739313 Msa0604280:intron 55.0%
! CTTTGTTTCAGAGGCAGGGC+CGG + chr4_2:49739360-49739379 None:intergenic 55.0%
TGTCCAAGGCTTGCATGCCT+AGG + chr4_2:49739538-49739557 None:intergenic 55.0%
CGACAGTGCCTGCAAGGATA+TGG + chr4_2:49739969-49739988 None:intergenic 55.0%
CAATAGCGACAGTGCCTGCA+AGG + chr4_2:49739975-49739994 None:intergenic 55.0%
!! CTTGGAGGAGCATTGCCTTG+TGG + chr4_2:49740488-49740507 None:intergenic 55.0%
!! AGGGCCGGCTCGAATGTAAA+GGG - chr4_2:49741389-49741408 Msa0604280:intron 55.0%
GCCCCAAAGATGAAGCCACG+TGG + chr4_2:49733880-49733899 None:intergenic 60.0%
!! CGTGGCTTCATCTTTGGGGC+GGG - chr4_2:49733880-49733899 Msa0604280:CDS 60.0%
!! GAGGGTGCTAAAGAGTGCCC+TGG + chr4_2:49736160-49736179 None:intergenic 60.0%
CTAGGAACGGCCCTGTTCAG+AGG + chr4_2:49738265-49738284 None:intergenic 60.0%
!! CGGCCCTGCCTCTGAAACAA+AGG - chr4_2:49739358-49739377 Msa0604280:intron 60.0%
!! GGAGGAGCATTGCCTTGTGG+AGG + chr4_2:49740485-49740504 None:intergenic 60.0%
!! CAGGGCCGGCTCGAATGTAA+AGG - chr4_2:49741388-49741407 Msa0604280:intron 60.0%
GCACTCCCGGCCTATGCCTA+GGG - chr4_2:49739334-49739353 Msa0604280:intron 65.0%
! GGCATAGGCCGGGAGTGCGA+CGG + chr4_2:49739332-49739351 None:intergenic 70.0%
CGCACTCCCGGCCTATGCCT+AGG - chr4_2:49739333-49739352 Msa0604280:intron 70.0%
TCCCGGCCTATGCCTAGGGC+CGG - chr4_2:49739338-49739357 Msa0604280:intron 70.0%
AATCTGGGCCCCCCACGGGC+TGG - chr4_2:49739298-49739317 Msa0604280:intron 75.0%
ATCTGGGCCCCCCACGGGCT+GGG - chr4_2:49739299-49739318 Msa0604280:intron 75.0%
GCCCTAGGCCGTCGCACTCC+CGG - chr4_2:49739321-49739340 Msa0604280:intron 75.0%
! GCCGGGAGTGCGACGGCCTA+GGG + chr4_2:49739325-49739344 None:intergenic 75.0%
GCCGGCCCTAGGCATAGGCC+GGG + chr4_2:49739342-49739361 None:intergenic 75.0%
GGCCGGCCCTAGGCATAGGC+CGG + chr4_2:49739343-49739362 None:intergenic 75.0%
GCAGGGCCGGCCCTAGGCAT+AGG + chr4_2:49739347-49739366 None:intergenic 75.0%
TCAGAGGCAGGGCCGGCCCT+AGG + chr4_2:49739353-49739372 None:intergenic 75.0%
! CCTAGGGCCCAGCCCGTGGG+GGG + chr4_2:49739309-49739328 None:intergenic 80.0%
! GCCTAGGGCCCAGCCCGTGG+GGG + chr4_2:49739310-49739329 None:intergenic 80.0%
! GGCCTAGGGCCCAGCCCGTG+GGG + chr4_2:49739311-49739330 None:intergenic 80.0%
! CGGCCTAGGGCCCAGCCCGT+GGG + chr4_2:49739312-49739331 None:intergenic 80.0%
! ACGGCCTAGGGCCCAGCCCG+TGG + chr4_2:49739313-49739332 None:intergenic 80.0%
!! GGCCGGGAGTGCGACGGCCT+AGG + chr4_2:49739326-49739345 None:intergenic 80.0%
!!! CCCCCCACGGGCTGGGCCCT+AGG - chr4_2:49739306-49739325 Msa0604280:intron 85.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 49733655 49741763 49733655 ID=Msa0604280;Name=Msa0604280
chr4_2 mRNA 49733655 49741763 49733655 ID=Msa0604280-mRNA-1;Parent=Msa0604280;Name=Msa0604280-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.25|0.30|1|1|1|13|201|544
chr4_2 exon 49733655 49734027 49733655 ID=Msa0604280-mRNA-1:exon:11539;Parent=Msa0604280-mRNA-1
chr4_2 exon 49734186 49734294 49734186 ID=Msa0604280-mRNA-1:exon:11538;Parent=Msa0604280-mRNA-1
chr4_2 exon 49734467 49734569 49734467 ID=Msa0604280-mRNA-1:exon:11537;Parent=Msa0604280-mRNA-1
chr4_2 exon 49734732 49734763 49734732 ID=Msa0604280-mRNA-1:exon:11536;Parent=Msa0604280-mRNA-1
chr4_2 exon 49734879 49735096 49734879 ID=Msa0604280-mRNA-1:exon:11535;Parent=Msa0604280-mRNA-1
chr4_2 exon 49737315 49737433 49737315 ID=Msa0604280-mRNA-1:exon:11534;Parent=Msa0604280-mRNA-1
chr4_2 exon 49737546 49737792 49737546 ID=Msa0604280-mRNA-1:exon:11533;Parent=Msa0604280-mRNA-1
chr4_2 exon 49737872 49737959 49737872 ID=Msa0604280-mRNA-1:exon:11532;Parent=Msa0604280-mRNA-1
chr4_2 exon 49738072 49738149 49738072 ID=Msa0604280-mRNA-1:exon:11531;Parent=Msa0604280-mRNA-1
chr4_2 exon 49738247 49738322 49738247 ID=Msa0604280-mRNA-1:exon:11530;Parent=Msa0604280-mRNA-1
chr4_2 exon 49739479 49739537 49739479 ID=Msa0604280-mRNA-1:exon:11529;Parent=Msa0604280-mRNA-1
chr4_2 exon 49740875 49740944 49740875 ID=Msa0604280-mRNA-1:exon:11528;Parent=Msa0604280-mRNA-1
chr4_2 exon 49741500 49741763 49741500 ID=Msa0604280-mRNA-1:exon:11527;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49741500 49741763 49741500 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49740875 49740944 49740875 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49739479 49739537 49739479 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49738247 49738322 49738247 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49738072 49738149 49738072 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49737872 49737959 49737872 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49737546 49737792 49737546 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49737315 49737433 49737315 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49734879 49735096 49734879 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49734732 49734763 49734732 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49734467 49734569 49734467 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49734186 49734294 49734186 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 CDS 49733856 49734027 49733856 ID=Msa0604280-mRNA-1:cds;Parent=Msa0604280-mRNA-1
chr4_2 three_prime_UTR 49733655 49733855 49733655 ID=Msa0604280-mRNA-1:three_prime_utr;Parent=Msa0604280-mRNA-1
Gene Sequence

>Msa0604280

ATGGATCGTAATAGGGGAAGTCCTCCTTCATGTATAAAGAGGCAGACACTAACACAAAAAAGTAAATTGGTCAAGCAGCACAAACCAAATATGGTATTCATTGGCTATTTCAATGTGGCCATGCTTGCTCTCTTTGTTGTTAGCTCAGTCACACTTACTTTCGCAAACGGAGTTGAAGTTTCAACTCCTCTTGACATCGGCAACCTGAGTCGGAGTAGTTTTCCACGTGGCTTCATCTTTGGGGCGGGATCGTCATCTTACCAATTTGAAGGTGCAGTAAAGGAAGGTGGTAGAGGACCAAGTATCTGGGACACCTTCACCCATCAACATCCAGAAAAAATAAGGGATGGAAGCAATGCAGACGTGACTGTTGACCAATACCATCGCTATAAGGAAGATGTTGGGATTATGAAGGATCAGAATATGGATTCCTATAGATTCTCAATCGCTTGGCCAAGAATACTCCCAAAGGGGAAGTTGAGTGGAGGCATAAATCATGAAGGAATCAAATATTACAACAACCTCATCAACGAATTGTTGGCTAATGGTATACAACCATTCGTAACTCTTTTTCATTGGGATCTTCCCCAAGTCTTAGAAGACGAGTATGGTGGTTTCTTAAGCTCTCTCATAATAAATGATTTTCGAGACTATGCTGATCTTTGCTTCAAGGAATTCGGAGATAGAGTGAAGTACTGGGTTACATTTAACGAGCCATGGTTGTTTAGTAATGGTGGATATGCAATGGGAACAACAGCACCAGGTCGATGTTCTAGCCCAACATGTTTGGGTGGTAATTCTGGCACAGAACCTTATATAGTTACACATAATCAAATTCTTGCTCATGCAGAAGCTGTACATGTGTATAAGATCAATTATCAGGCATATCAAAAGGGCAAGATAGGCATAACATTGGTAACAAATTGGTTCATACCACTTGGAAACAATAGCATACCCGATCAAAAGGCTGCCAAGAGATCACTTGACTTCCAATTTGGATGGTTTATGGAACCTCTAACAACCGGAGATTATCCTAAAAGCATGCAAGCCATAGTGAAAAACCGATTGCCTAAGTTCACTAAATTGCAATCAAAGCTAGTGAATGGTTCATTTGATTTTATTGGTATAAACTATTACTCTTCTAGCTATATTAACCATTTACCTCCACAAGGCAATGCTCCTCCAAGTTACTTAACAGATCCTATGACTAATACTTCATTTGAACAAAATGGAAAACCTCTAGGTCCAAGGGCTGCTTCAATTTGGATATACGTTTATCCAAGAGGACTTCGAGATCTTTTGTTATACACTAAGGAGAAATACAACAATCCTGTGGTCTACATTACTGAAAATGGTATGAATGAATTCAACGACGCAACACTTTCTGTAGAAGAAGCTCTTTTGGATACTTACAGAATTGATTACTATTATCGACATTTCTTCTATATTCGTTCCGCAATCAAGGCCGGCTCGAATGTAAAGGGATTTTTCGCATGGTCATTTTTGGACTGTAATGAATGGTTTGCAGGCTTTACTGTTCGTTTTGGATTAAACTTTGTAGATTACAATGATGGATTGAAAAGGTACCCAAAGCTTTCTGCTCAATGGTACAAGAACTTTCTCAAAAGAAACTAG
Protein sequence

>Msa0604280

MDRNRGSPPSCIKRQTLTQKSKLVKQHKPNMVFIGYFNVAMLALFVVSSVTLTFANGVEVSTPLDIGNLSRSSFPRGFIFGAGSSSYQFEGAVKEGGRGPSIWDTFTHQHPEKIRDGSNADVTVDQYHRYKEDVGIMKDQNMDSYRFSIAWPRILPKGKLSGGINHEGIKYYNNLINELLANGIQPFVTLFHWDLPQVLEDEYGGFLSSLIINDFRDYADLCFKEFGDRVKYWVTFNEPWLFSNGGYAMGTTAPGRCSSPTCLGGNSGTEPYIVTHNQILAHAEAVHVYKINYQAYQKGKIGITLVTNWFIPLGNNSIPDQKAAKRSLDFQFGWFMEPLTTGDYPKSMQAIVKNRLPKFTKLQSKLVNGSFDFIGINYYSSSYINHLPPQGNAPPSYLTDPMTNTSFEQNGKPLGPRAASIWIYVYPRGLRDLLLYTKEKYNNPVVYITENGMNEFNDATLSVEEALLDTYRIDYYYRHFFYIRSAIKAGSNVKGFFAWSFLDCNEWFAGFTVRFGLNFVDYNDGLKRYPKLSAQWYKNFLKRN*