Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1133630 | XP_039684299.1 | 96.667 | 180 | 4 | 1 | 1 | 178 | 1 | 180 | 1.30e-123 | 357 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1133630 | sp|O24543|AX22E_VIGRR | 73.770 | 183 | 39 | 5 | 1 | 178 | 1 | 179 | 7.93e-82 | 243 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1133630 | A0A396HA94 | 96.667 | 180 | 4 | 1 | 1 | 178 | 1 | 180 | 6.22e-124 | 357 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1133630 | TR | AUX/IAA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000940 | Msa1133630 | 0.997389 | 1.143703e-241 | -8.615850e-47 |
| Msa0025810 | Msa1133630 | 0.807032 | 6.161021e-50 | -8.615850e-47 |
| Msa0094010 | Msa1133630 | 0.821580 | 3.744722e-53 | -8.615850e-47 |
| Msa0100800 | Msa1133630 | 0.820720 | 5.909350e-53 | -8.615850e-47 |
| Msa0609970 | Msa1133630 | 0.814527 | 1.472427e-51 | -8.615850e-47 |
| Msa0615660 | Msa1133630 | 0.807760 | 4.318892e-50 | -8.615850e-47 |
| Msa0625790 | Msa1133630 | 0.812426 | 4.265544e-51 | -8.615850e-47 |
| Msa0639120 | Msa1133630 | 0.809562 | 1.779639e-50 | -8.615850e-47 |
| Msa0658480 | Msa1133630 | 0.806832 | 6.793340e-50 | -8.615850e-47 |
| Msa0664760 | Msa1133630 | 0.804779 | 1.833785e-49 | -8.615850e-47 |
| Msa0704800 | Msa1133630 | 0.809914 | 1.495214e-50 | -8.615850e-47 |
| Msa0707390 | Msa1133630 | 0.832836 | 7.529380e-56 | -8.615850e-47 |
| Msa0742510 | Msa1133630 | 0.811323 | 7.417041e-51 | -8.615850e-47 |
| Msa0797750 | Msa1133630 | 0.807030 | 6.168375e-50 | -8.615850e-47 |
| Msa0824730 | Msa1133630 | 0.807491 | 4.926400e-50 | -8.615850e-47 |
| Msa0131450 | Msa1133630 | 0.805597 | 1.236249e-49 | -8.615850e-47 |
| Msa0152840 | Msa1133630 | 0.801489 | 8.788292e-49 | -8.615850e-47 |
| Msa0165900 | Msa1133630 | 0.801016 | 1.098315e-48 | -8.615850e-47 |
| Msa0212750 | Msa1133630 | -0.834242 | 3.356607e-56 | -8.615850e-47 |
| Msa0370160 | Msa1133630 | -0.800509 | 1.394309e-48 | -8.615850e-47 |
| Msa0437690 | Msa1133630 | -0.801936 | 7.115302e-49 | -8.615850e-47 |
| Msa0449820 | Msa1133630 | 0.801817 | 7.527728e-49 | -8.615850e-47 |
| Msa0463500 | Msa1133630 | 0.819697 | 1.013490e-52 | -8.615850e-47 |
| Msa1033520 | Msa1133630 | 0.812142 | 4.919227e-51 | -8.615850e-47 |
| Msa1036320 | Msa1133630 | 0.812590 | 3.927650e-51 | -8.615850e-47 |
| Msa1070050 | Msa1133630 | 0.801687 | 8.004143e-49 | -8.615850e-47 |
| Msa1071660 | Msa1133630 | 0.801017 | 1.097962e-48 | -8.615850e-47 |
| Msa1073400 | Msa1133630 | 0.837293 | 5.661709e-57 | -8.615850e-47 |
| Msa1073830 | Msa1133630 | 0.810501 | 1.117297e-50 | -8.615850e-47 |
| Msa1076600 | Msa1133630 | 0.864381 | 1.266772e-64 | -8.615850e-47 |
| Msa1113590 | Msa1133630 | 0.811565 | 6.569303e-51 | -8.615850e-47 |
| Msa1113880 | Msa1133630 | 0.815385 | 9.499762e-52 | -8.615850e-47 |
| Msa1114040 | Msa1133630 | 0.842794 | 2.079848e-58 | -8.615850e-47 |
| Msa1133630 | Msa1158770 | 0.804360 | 2.243059e-49 | -8.615850e-47 |
| Msa1133630 | Msa1160360 | 0.813521 | 2.453713e-51 | -8.615850e-47 |
| Msa1133630 | Msa1192850 | 0.807027 | 6.175401e-50 | -8.615850e-47 |
| Msa1133630 | Msa1193950 | -0.822737 | 2.018150e-53 | -8.615850e-47 |
| Msa1133630 | Msa1201830 | 0.816603 | 5.078536e-52 | -8.615850e-47 |
| Msa1133630 | Msa1246530 | -0.821967 | 3.045996e-53 | -8.615850e-47 |
| Msa1133630 | Msa1253810 | 0.800574 | 1.352246e-48 | -8.615850e-47 |
| Msa1133630 | Msa1299360 | 0.808359 | 3.218898e-50 | -8.615850e-47 |
| Msa1133630 | Msa1310690 | 0.805258 | 1.455699e-49 | -8.615850e-47 |
| Msa1133630 | Msa1327490 | 0.807669 | 4.515449e-50 | -8.615850e-47 |
| Msa1133630 | Msa1338610 | 0.804201 | 2.420621e-49 | -8.615850e-47 |
| Msa1133630 | Msa1373880 | 0.991248 | 1.188992e-186 | -8.615850e-47 |
| Msa1133630 | Msa1438450 | 0.801320 | 9.520040e-49 | -8.615850e-47 |
| Msa1133630 | Msa1438640 | 0.814241 | 1.702873e-51 | -8.615850e-47 |
| Msa0534080 | Msa1133630 | 0.806706 | 7.220567e-50 | -8.615850e-47 |
| Msa0578090 | Msa1133630 | 0.820259 | 7.539785e-53 | -8.615850e-47 |
| Msa0959010 | Msa1133630 | 0.802067 | 6.687187e-49 | -8.615850e-47 |
| Msa0966720 | Msa1133630 | -0.834221 | 3.397165e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1133630 | MtrunA17_Chr7g0260621 | 96.667 | 180 | 4 | 1 | 1 | 178 | 1 | 180 | 1.20e-127 | 357 |
| Msa1133630 | MtrunA17_Chr1g0184801 | 71.676 | 173 | 42 | 5 | 11 | 179 | 12 | 181 | 7.66e-80 | 236 |
| Msa1133630 | MtrunA17_Chr1g0166011 | 60.947 | 169 | 57 | 4 | 11 | 178 | 1 | 161 | 4.65e-66 | 200 |
| Msa1133630 | MtrunA17_Chr1g0187961 | 56.471 | 170 | 49 | 5 | 11 | 178 | 11 | 157 | 1.15e-59 | 184 |
| Msa1133630 | MtrunA17_Chr6g0486121 | 44.231 | 208 | 77 | 7 | 11 | 179 | 15 | 222 | 3.81e-43 | 144 |
| Msa1133630 | MtrunA17_Chr1g0190711 | 42.130 | 216 | 71 | 7 | 10 | 179 | 14 | 221 | 7.77e-40 | 135 |
| Msa1133630 | MtrunA17_Chr1g0187921 | 41.709 | 199 | 82 | 7 | 11 | 178 | 8 | 203 | 5.69e-38 | 130 |
| Msa1133630 | MtrunA17_Chr8g0390281 | 40.329 | 243 | 76 | 10 | 6 | 179 | 19 | 261 | 1.34e-37 | 130 |
| Msa1133630 | MtrunA17_Chr5g0428141 | 37.647 | 255 | 77 | 6 | 6 | 178 | 60 | 314 | 1.47e-37 | 132 |
| Msa1133630 | MtrunA17_Chr1g0184791 | 39.631 | 217 | 81 | 7 | 2 | 178 | 6 | 212 | 6.43e-37 | 127 |
| Msa1133630 | MtrunA17_Chr8g0366881 | 37.349 | 249 | 75 | 8 | 11 | 178 | 67 | 315 | 9.66e-37 | 130 |
| Msa1133630 | MtrunA17_Chr2g0331411 | 45.695 | 151 | 58 | 3 | 52 | 178 | 143 | 293 | 4.68e-36 | 127 |
| Msa1133630 | MtrunA17_Chr5g0411501 | 35.659 | 258 | 82 | 10 | 6 | 179 | 46 | 303 | 2.20e-34 | 123 |
| Msa1133630 | MtrunA17_Chr7g0260641 | 38.095 | 210 | 85 | 7 | 11 | 178 | 11 | 217 | 1.32e-31 | 114 |
| Msa1133630 | MtrunA17_Chr4g0004181 | 43.558 | 163 | 64 | 4 | 16 | 178 | 11 | 145 | 8.57e-31 | 110 |
| Msa1133630 | MtrunA17_Chr2g0330951 | 31.858 | 226 | 90 | 7 | 14 | 178 | 15 | 237 | 2.11e-23 | 93.6 |
| Msa1133630 | MtrunA17_Chr8g0340731 | 32.335 | 167 | 70 | 4 | 51 | 178 | 81 | 243 | 1.63e-20 | 85.9 |
| Msa1133630 | MtrunA17_Chr2g0332141 | 49.398 | 83 | 29 | 2 | 108 | 179 | 8 | 88 | 1.76e-20 | 82.0 |
| Msa1133630 | MtrunA17_Chr1g0183251 | 32.903 | 155 | 46 | 4 | 81 | 178 | 82 | 235 | 3.78e-18 | 79.3 |
| Msa1133630 | MtrunA17_Chr4g0069561 | 37.143 | 105 | 62 | 2 | 76 | 179 | 66 | 167 | 2.79e-17 | 75.5 |
| Msa1133630 | MtrunA17_Chr7g0270851 | 41.121 | 107 | 56 | 3 | 74 | 179 | 42 | 142 | 4.31e-17 | 74.3 |
| Msa1133630 | MtrunA17_Chr1g0193861 | 39.000 | 100 | 50 | 3 | 85 | 179 | 64 | 157 | 2.43e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1133630 | AT5G43700.1 | 57.059 | 170 | 53 | 3 | 10 | 178 | 10 | 160 | 1.35e-61 | 189 |
| Msa1133630 | AT1G04240.1 | 55.114 | 176 | 57 | 4 | 10 | 178 | 4 | 164 | 2.20e-59 | 184 |
| Msa1133630 | AT1G04240.2 | 54.444 | 180 | 60 | 4 | 6 | 178 | 100 | 264 | 7.69e-59 | 186 |
| Msa1133630 | AT3G23030.1 | 55.172 | 174 | 39 | 4 | 11 | 178 | 9 | 149 | 6.19e-54 | 169 |
| Msa1133630 | AT3G23030.2 | 55.172 | 174 | 39 | 4 | 11 | 178 | 96 | 236 | 2.44e-53 | 171 |
| Msa1133630 | AT4G14560.1 | 56.548 | 168 | 45 | 3 | 11 | 178 | 7 | 146 | 1.47e-50 | 160 |
| Msa1133630 | AT3G04730.1 | 43.062 | 209 | 73 | 7 | 10 | 178 | 1 | 203 | 5.70e-42 | 140 |
| Msa1133630 | AT1G04250.1 | 41.837 | 196 | 80 | 6 | 11 | 178 | 7 | 196 | 6.32e-41 | 138 |
| Msa1133630 | AT4G29080.1 | 42.128 | 235 | 69 | 10 | 11 | 178 | 38 | 272 | 8.78e-41 | 139 |
| Msa1133630 | AT3G23050.2 | 40.291 | 206 | 85 | 6 | 10 | 178 | 5 | 209 | 5.82e-39 | 132 |
| Msa1133630 | AT4G14550.3 | 41.327 | 196 | 86 | 5 | 11 | 178 | 1 | 195 | 6.29e-39 | 132 |
| Msa1133630 | AT4G14550.1 | 41.327 | 196 | 86 | 5 | 11 | 178 | 1 | 195 | 8.66e-39 | 132 |
| Msa1133630 | AT4G14550.2 | 41.327 | 196 | 86 | 5 | 11 | 178 | 1 | 195 | 1.04e-38 | 132 |
| Msa1133630 | AT3G23050.1 | 40.291 | 206 | 85 | 6 | 10 | 178 | 5 | 209 | 1.28e-38 | 132 |
| Msa1133630 | AT2G22670.3 | 35.833 | 240 | 82 | 8 | 11 | 178 | 47 | 286 | 1.26e-31 | 116 |
| Msa1133630 | AT2G22670.1 | 35.833 | 240 | 82 | 8 | 11 | 178 | 47 | 286 | 1.26e-31 | 116 |
| Msa1133630 | AT2G22670.5 | 35.833 | 240 | 82 | 8 | 11 | 178 | 47 | 286 | 1.27e-31 | 116 |
| Msa1133630 | AT2G22670.2 | 35.833 | 240 | 82 | 8 | 11 | 178 | 47 | 286 | 1.27e-31 | 116 |
| Msa1133630 | AT2G22670.4 | 35.833 | 240 | 82 | 8 | 11 | 178 | 64 | 303 | 1.63e-31 | 116 |
| Msa1133630 | AT1G52830.1 | 44.578 | 166 | 78 | 8 | 16 | 179 | 11 | 164 | 2.18e-31 | 112 |
| Msa1133630 | AT5G65670.2 | 49.194 | 124 | 40 | 5 | 78 | 178 | 180 | 303 | 2.34e-30 | 113 |
| Msa1133630 | AT5G65670.1 | 49.194 | 124 | 40 | 5 | 78 | 178 | 180 | 303 | 2.42e-30 | 113 |
| Msa1133630 | AT1G15580.1 | 41.818 | 165 | 62 | 5 | 16 | 179 | 13 | 144 | 3.01e-30 | 108 |
| Msa1133630 | AT3G15540.1 | 41.212 | 165 | 87 | 4 | 16 | 178 | 11 | 167 | 1.90e-29 | 107 |
| Msa1133630 | AT3G16500.2 | 35.096 | 208 | 86 | 8 | 14 | 179 | 36 | 236 | 5.78e-25 | 97.8 |
| Msa1133630 | AT3G23050.3 | 38.415 | 164 | 75 | 5 | 10 | 148 | 5 | 167 | 5.97e-25 | 95.5 |
| Msa1133630 | AT3G16500.1 | 35.096 | 208 | 86 | 8 | 14 | 179 | 36 | 236 | 6.11e-25 | 97.8 |
| Msa1133630 | AT4G14550.4 | 39.726 | 146 | 72 | 3 | 11 | 141 | 1 | 145 | 4.41e-24 | 93.6 |
| Msa1133630 | AT4G28640.3 | 39.716 | 141 | 50 | 5 | 73 | 178 | 80 | 220 | 2.50e-21 | 87.8 |
| Msa1133630 | AT4G28640.1 | 39.716 | 141 | 50 | 5 | 73 | 178 | 80 | 220 | 2.70e-21 | 87.8 |
| Msa1133630 | AT4G28640.2 | 39.716 | 141 | 50 | 5 | 73 | 178 | 80 | 220 | 1.97e-20 | 86.3 |
| Msa1133630 | AT1G51950.1 | 30.144 | 209 | 89 | 7 | 14 | 179 | 40 | 234 | 2.28e-20 | 85.5 |
| Msa1133630 | AT1G04550.2 | 36.076 | 158 | 64 | 4 | 58 | 179 | 47 | 203 | 1.93e-19 | 82.8 |
| Msa1133630 | AT3G17600.1 | 41.237 | 97 | 50 | 2 | 85 | 178 | 48 | 140 | 1.99e-19 | 80.9 |
| Msa1133630 | AT2G33310.3 | 37.267 | 161 | 58 | 5 | 56 | 179 | 56 | 210 | 3.03e-19 | 82.4 |
| Msa1133630 | AT2G33310.1 | 37.267 | 161 | 58 | 5 | 56 | 179 | 56 | 210 | 3.03e-19 | 82.4 |
| Msa1133630 | AT2G33310.2 | 35.220 | 159 | 66 | 4 | 58 | 179 | 53 | 211 | 4.21e-19 | 82.0 |
| Msa1133630 | AT2G46990.2 | 46.575 | 73 | 39 | 0 | 107 | 179 | 85 | 157 | 1.47e-16 | 73.2 |
| Msa1133630 | AT2G46990.1 | 46.575 | 73 | 39 | 0 | 107 | 179 | 85 | 157 | 2.55e-16 | 73.2 |
| Msa1133630 | AT3G62100.2 | 42.683 | 82 | 47 | 0 | 98 | 179 | 74 | 155 | 4.85e-16 | 72.0 |
| Msa1133630 | AT3G62100.1 | 42.683 | 82 | 47 | 0 | 98 | 179 | 74 | 155 | 7.88e-16 | 71.6 |
| Msa1133630 | AT1G04100.1 | 35.333 | 150 | 49 | 5 | 78 | 179 | 90 | 239 | 8.59e-16 | 73.2 |
| Msa1133630 | AT5G25890.1 | 32.061 | 131 | 70 | 4 | 56 | 178 | 27 | 146 | 1.12e-15 | 71.2 |
| Msa1133630 | AT1G80390.1 | 35.433 | 127 | 59 | 4 | 16 | 142 | 19 | 122 | 2.19e-14 | 67.0 |
| Msa1133630 | AT4G32280.2 | 45.205 | 73 | 35 | 2 | 107 | 179 | 118 | 185 | 3.62e-11 | 60.1 |
Find 62 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTATTTATTTATAATTT+TGG | 0.106018 | 7_3:-87250678 | Msa1133630:three_prime_UTR |
| CTCAGAACTTCTTAAGGTTT+TGG | 0.201758 | 7_3:-87251270 | Msa1133630:CDS |
| CTTTATTTATATAGAGATTA+AGG | 0.258286 | 7_3:-87251820 | Msa1133630:five_prime_UTR |
| ATAGAGTTTGGATTTGGATT+TGG | 0.302329 | 7_3:-87251859 | Msa1133630:five_prime_UTR |
| TTCTTCCTAAAAGATCTAAT+TGG | 0.303224 | 7_3:+87251401 | None:intergenic |
| AATTAATTATTATCAAGAAA+TGG | 0.308561 | 7_3:-87251791 | Msa1133630:exon |
| CAGAATTTATCAATAGAGTT+TGG | 0.318162 | 7_3:-87251871 | Msa1133630:five_prime_UTR |
| TTATCAATAGAGTTTGGATT+TGG | 0.319954 | 7_3:-87251865 | Msa1133630:five_prime_UTR |
| AGAATTGATGATTGACGAAA+TGG | 0.360169 | 7_3:+87251927 | None:intergenic |
| ATTAATTATTATCAAGAAAT+GGG | 0.388582 | 7_3:-87251790 | Msa1133630:exon |
| AAAGGCTTGAATTAAGAAAT+CGG | 0.390933 | 7_3:+87250865 | None:intergenic |
| ATTAACAAAAGCACAAAATC+AGG | 0.391339 | 7_3:-87250945 | Msa1133630:three_prime_UTR |
| GAGTTACTCAGAACTTCTTA+AGG | 0.392999 | 7_3:-87251276 | Msa1133630:CDS |
| TTATGTATGACTTACTTTGT+AGG | 0.395482 | 7_3:+87251544 | None:intergenic |
| AATATGTTCAAGTGTACATT+TGG | 0.401164 | 7_3:-87251245 | Msa1133630:CDS |
| GTCATGATCACTTGTGGTTG+TGG | 0.404340 | 7_3:+87251582 | None:intergenic |
| TCTCTCTCAAATATCTAGAA+AGG | 0.408067 | 7_3:+87250847 | None:intergenic |
| GGATTTGGATTTGGATTTGA+AGG | 0.422068 | 7_3:-87251850 | Msa1133630:five_prime_UTR |
| ATCACCAGAGCTCTTAGAAA+TGG | 0.429190 | 7_3:+87251612 | None:intergenic |
| GAATTAAGATTGGGATTGCC+TGG | 0.450216 | 7_3:-87251724 | Msa1133630:CDS |
| GTGAAAGGATCAGAAGCAAA+AGG | 0.452877 | 7_3:-87250993 | Msa1133630:three_prime_UTR |
| GATTGTTCATCATCACTTCC+AGG | 0.460419 | 7_3:+87251706 | None:intergenic |
| AATTTAAGAACTTACTTCCA+TGG | 0.462324 | 7_3:+87251131 | None:intergenic |
| CAAGGACACAGAATTAAGAT+TGG | 0.462867 | 7_3:-87251734 | Msa1133630:CDS |
| GTTCTATAGAGGTGGCTTTG+AGG | 0.471957 | 7_3:-87250889 | Msa1133630:three_prime_UTR |
| TTGTAGGTTGGACAGTGTCT+TGG | 0.481019 | 7_3:+87251560 | None:intergenic |
| GTATGACTTACTTTGTAGGT+TGG | 0.482291 | 7_3:+87251548 | None:intergenic |
| CAACAAAATAAAGTGGAAGA+TGG | 0.489467 | 7_3:-87251371 | Msa1133630:CDS |
| AATGTATGTGAAAGTGAGTA+TGG | 0.492807 | 7_3:-87251342 | Msa1133630:CDS |
| TGAACATGCTCCTACTTATG+AGG | 0.497905 | 7_3:-87251189 | Msa1133630:CDS |
| TTCCTAAAAGATCTAATTGG+TGG | 0.498613 | 7_3:+87251404 | None:intergenic |
| GAATGAGATGAGAAGAAGCT+AGG | 0.499678 | 7_3:+87251949 | None:intergenic |
| AAGGCTTGAATTAAGAAATC+GGG | 0.511357 | 7_3:+87250866 | None:intergenic |
| AAGGACACAGAATTAAGATT+GGG | 0.515527 | 7_3:-87251733 | Msa1133630:CDS |
| ATGAGGATAAAGATGGTGAT+TGG | 0.518002 | 7_3:-87251172 | Msa1133630:CDS |
| GAGTCCATTTCTAAGAGCTC+TGG | 0.522835 | 7_3:-87251616 | Msa1133630:CDS |
| GTGAATATTCAGAAAGAGAA+GGG | 0.526820 | 7_3:-87251223 | Msa1133630:CDS |
| AATAAAGTGGAAGATGGAAG+TGG | 0.532994 | 7_3:-87251365 | Msa1133630:CDS |
| GCAGGGTACAAGTAGTTGGA+TGG | 0.541049 | 7_3:-87251427 | Msa1133630:intron |
| GATGGTGATTGGATGCTAGT+TGG | 0.542154 | 7_3:-87251161 | Msa1133630:CDS |
| TAGTTTACAACAAAATAAAG+TGG | 0.543416 | 7_3:-87251378 | Msa1133630:CDS |
| GGTGAATATTCAGAAAGAGA+AGG | 0.544646 | 7_3:-87251224 | Msa1133630:CDS |
| CCATCTTTATCCTCATAAGT+AGG | 0.549431 | 7_3:+87251179 | None:intergenic |
| CCTACTTATGAGGATAAAGA+TGG | 0.550050 | 7_3:-87251179 | Msa1133630:CDS |
| GAGCAGAGAGAGTTCTATAG+AGG | 0.551485 | 7_3:-87250900 | Msa1133630:three_prime_UTR |
| TGAAAGGATCAGAAGCAAAA+GGG | 0.552833 | 7_3:-87250992 | Msa1133630:three_prime_UTR |
| TGTTTATATCCTCATGCAAG+AGG | 0.561614 | 7_3:-87251025 | Msa1133630:three_prime_UTR |
| ATTAAAATATATACACAACA+TGG | 0.565005 | 7_3:-87250730 | Msa1133630:three_prime_UTR |
| ATGTACAATCAAAATAGCAT+TGG | 0.581894 | 7_3:+87250804 | None:intergenic |
| GTCTTGGTCATGATCACTTG+TGG | 0.583842 | 7_3:+87251576 | None:intergenic |
| GATGATGAACAATCATGTAA+TGG | 0.589704 | 7_3:-87251697 | Msa1133630:CDS |
| TATGTGAAAGTGAGTATGGC+TGG | 0.590400 | 7_3:-87251338 | Msa1133630:CDS |
| CAGAGAGAGTTCTATAGAGG+TGG | 0.594735 | 7_3:-87250897 | Msa1133630:three_prime_UTR |
| AGGATCAGAAGCAAAAGGGT+TGG | 0.596397 | 7_3:-87250988 | Msa1133630:three_prime_UTR |
| TCAGAAAGAGAAGGGTACAA+TGG | 0.598816 | 7_3:-87251215 | Msa1133630:CDS |
| TGGCTGGTGCACCTTACTTG+AGG | 0.603488 | 7_3:-87251322 | Msa1133630:CDS |
| TTAAAATATATACACAACAT+GGG | 0.610245 | 7_3:-87250729 | Msa1133630:three_prime_UTR |
| TGCTAGTTGGAGATGTTCCA+TGG | 0.610466 | 7_3:-87251148 | Msa1133630:CDS |
| AAGAGGTTGAAGATAGTGAA+AGG | 0.619298 | 7_3:-87251008 | Msa1133630:three_prime_UTR |
| TATCTTCAACCTCTTGCATG+AGG | 0.646730 | 7_3:+87251016 | None:intergenic |
| GATGAACAATCATGTAATGG+TGG | 0.660059 | 7_3:-87251694 | Msa1133630:CDS |
| AGAGTTGATTATTAATCACA+AGG | 0.665606 | 7_3:-87251752 | Msa1133630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTATTTATTTATAATTT+TGG | - | chr7_3:87251901-87251920 | Msa1133630:five_prime_UTR | 0.0% |
| !! | AATTAATTATTATCAAGAAA+TGG | - | chr7_3:87250788-87250807 | Msa1133630:three_prime_UTR | 10.0% |
| !! | ATTAATTATTATCAAGAAAT+GGG | - | chr7_3:87250789-87250808 | Msa1133630:three_prime_UTR | 10.0% |
| !!! | CTTTATTTATATAGAGATTA+AGG | - | chr7_3:87250759-87250778 | Msa1133630:three_prime_UTR | 15.0% |
| !! | ATCAACATATATATGAATAA+TGG | - | chr7_3:87251057-87251076 | Msa1133630:three_prime_UTR | 15.0% |
| !!! | ATAAACTTTTGTTGATTTTT+TGG | - | chr7_3:87251091-87251110 | Msa1133630:three_prime_UTR | 15.0% |
| !!! | TAAACTTTTGTTGATTTTTT+GGG | - | chr7_3:87251092-87251111 | Msa1133630:three_prime_UTR | 15.0% |
| !!! | TTTAATTATTTTTGGTTTCA+TGG | - | chr7_3:87251489-87251508 | Msa1133630:intron | 15.0% |
| !! | ATTAAAATATATACACAACA+TGG | - | chr7_3:87251849-87251868 | Msa1133630:five_prime_UTR | 15.0% |
| !! | TTAAAATATATACACAACAT+GGG | - | chr7_3:87251850-87251869 | Msa1133630:five_prime_UTR | 15.0% |
| !!! | AAATTCATGAATATTTTTGC+AGG | - | chr7_3:87251134-87251153 | Msa1133630:exon | 20.0% |
| !!! | AATTCATGAATATTTTTGCA+GGG | - | chr7_3:87251135-87251154 | Msa1133630:exon | 20.0% |
| !! | TAGTTTACAACAAAATAAAG+TGG | - | chr7_3:87251201-87251220 | Msa1133630:CDS | 20.0% |
| ! | GAAAAATCTGATTAAGTCTT+TGG | + | chr7_3:87250689-87250708 | None:intergenic | 25.0% |
| ! | CAGAATTTATCAATAGAGTT+TGG | - | chr7_3:87250708-87250727 | Msa1133630:three_prime_UTR | 25.0% |
| ! | TTATCAATAGAGTTTGGATT+TGG | - | chr7_3:87250714-87250733 | Msa1133630:three_prime_UTR | 25.0% |
| !! | AGAGTTGATTATTAATCACA+AGG | - | chr7_3:87250827-87250846 | Msa1133630:three_prime_UTR | 25.0% |
| ! | TTATGTATGACTTACTTTGT+AGG | + | chr7_3:87251038-87251057 | None:intergenic | 25.0% |
| ! | TTCTTCCTAAAAGATCTAAT+TGG | + | chr7_3:87251181-87251200 | None:intergenic | 25.0% |
| ! | AATATGTTCAAGTGTACATT+TGG | - | chr7_3:87251334-87251353 | Msa1133630:CDS | 25.0% |
| ! | AATTTAAGAACTTACTTCCA+TGG | + | chr7_3:87251451-87251470 | None:intergenic | 25.0% |
| !! | ATTAACAAAAGCACAAAATC+AGG | - | chr7_3:87251634-87251653 | Msa1133630:CDS | 25.0% |
| ! | AAAGGCTTGAATTAAGAAAT+CGG | + | chr7_3:87251717-87251736 | None:intergenic | 25.0% |
| ! | ATGTACAATCAAAATAGCAT+TGG | + | chr7_3:87251778-87251797 | None:intergenic | 25.0% |
| ! | AGAATTGATGATTGACGAAA+TGG | + | chr7_3:87250655-87250674 | None:intergenic | 30.0% |
| ATAGAGTTTGGATTTGGATT+TGG | - | chr7_3:87250720-87250739 | Msa1133630:three_prime_UTR | 30.0% | |
| AAGGACACAGAATTAAGATT+GGG | - | chr7_3:87250846-87250865 | Msa1133630:three_prime_UTR | 30.0% | |
| GATGATGAACAATCATGTAA+TGG | - | chr7_3:87250882-87250901 | Msa1133630:three_prime_UTR | 30.0% | |
| TTCCTAAAAGATCTAATTGG+TGG | + | chr7_3:87251178-87251197 | None:intergenic | 30.0% | |
| CAACAAAATAAAGTGGAAGA+TGG | - | chr7_3:87251208-87251227 | Msa1133630:CDS | 30.0% | |
| AATGTATGTGAAAGTGAGTA+TGG | - | chr7_3:87251237-87251256 | Msa1133630:CDS | 30.0% | |
| ! | AGATCTATTTTCCTCAAGTA+AGG | + | chr7_3:87251271-87251290 | None:intergenic | 30.0% |
| GTGAATATTCAGAAAGAGAA+GGG | - | chr7_3:87251356-87251375 | Msa1133630:CDS | 30.0% | |
| ! | GTGCTTTTGTTAATTTTGTG+AGG | + | chr7_3:87251628-87251647 | None:intergenic | 30.0% |
| AAGGCTTGAATTAAGAAATC+GGG | + | chr7_3:87251716-87251735 | None:intergenic | 30.0% | |
| TCTCTCTCAAATATCTAGAA+AGG | + | chr7_3:87251735-87251754 | None:intergenic | 30.0% | |
| GGATTTGGATTTGGATTTGA+AGG | - | chr7_3:87250729-87250748 | Msa1133630:three_prime_UTR | 35.0% | |
| CAAGGACACAGAATTAAGAT+TGG | - | chr7_3:87250845-87250864 | Msa1133630:three_prime_UTR | 35.0% | |
| GATGAACAATCATGTAATGG+TGG | - | chr7_3:87250885-87250904 | Msa1133630:three_prime_UTR | 35.0% | |
| GTATGACTTACTTTGTAGGT+TGG | + | chr7_3:87251034-87251053 | None:intergenic | 35.0% | |
| AATAAAGTGGAAGATGGAAG+TGG | - | chr7_3:87251214-87251233 | Msa1133630:CDS | 35.0% | |
| GAGTTACTCAGAACTTCTTA+AGG | - | chr7_3:87251303-87251322 | Msa1133630:CDS | 35.0% | |
| ! | CTCAGAACTTCTTAAGGTTT+TGG | - | chr7_3:87251309-87251328 | Msa1133630:CDS | 35.0% |
| GGTGAATATTCAGAAAGAGA+AGG | - | chr7_3:87251355-87251374 | Msa1133630:CDS | 35.0% | |
| CCATCTTTATCCTCATAAGT+AGG | + | chr7_3:87251403-87251422 | None:intergenic | 35.0% | |
| CCTACTTATGAGGATAAAGA+TGG | - | chr7_3:87251400-87251419 | Msa1133630:CDS | 35.0% | |
| ATGAGGATAAAGATGGTGAT+TGG | - | chr7_3:87251407-87251426 | Msa1133630:CDS | 35.0% | |
| TGTTTATATCCTCATGCAAG+AGG | - | chr7_3:87251554-87251573 | Msa1133630:intron | 35.0% | |
| AAGAGGTTGAAGATAGTGAA+AGG | - | chr7_3:87251571-87251590 | Msa1133630:CDS | 35.0% | |
| TGAAAGGATCAGAAGCAAAA+GGG | - | chr7_3:87251587-87251606 | Msa1133630:CDS | 35.0% | |
| GAATGAGATGAGAAGAAGCT+AGG | + | chr7_3:87250633-87250652 | None:intergenic | 40.0% | |
| GAATTAAGATTGGGATTGCC+TGG | - | chr7_3:87250855-87250874 | Msa1133630:three_prime_UTR | 40.0% | |
| GATTGTTCATCATCACTTCC+AGG | + | chr7_3:87250876-87250895 | None:intergenic | 40.0% | |
| ATCACCAGAGCTCTTAGAAA+TGG | + | chr7_3:87250970-87250989 | None:intergenic | 40.0% | |
| ! | TTTTGCAGGGTACAAGTAGT+TGG | - | chr7_3:87251148-87251167 | Msa1133630:CDS | 40.0% |
| ! | GGCCACCAATTAGATCTTTT+AGG | - | chr7_3:87251173-87251192 | Msa1133630:CDS | 40.0% |
| TATGTGAAAGTGAGTATGGC+TGG | - | chr7_3:87251241-87251260 | Msa1133630:CDS | 40.0% | |
| ! | TCAGAAAGAGAAGGGTACAA+TGG | - | chr7_3:87251364-87251383 | Msa1133630:CDS | 40.0% |
| TGAACATGCTCCTACTTATG+AGG | - | chr7_3:87251390-87251409 | Msa1133630:CDS | 40.0% | |
| TATCTTCAACCTCTTGCATG+AGG | + | chr7_3:87251566-87251585 | None:intergenic | 40.0% | |
| GTGAAAGGATCAGAAGCAAA+AGG | - | chr7_3:87251586-87251605 | Msa1133630:CDS | 40.0% | |
| ! | GAGTCCATTTCTAAGAGCTC+TGG | - | chr7_3:87250963-87250982 | Msa1133630:three_prime_UTR | 45.0% |
| GTCATGATCACTTGTGGTTG+TGG | + | chr7_3:87251000-87251019 | None:intergenic | 45.0% | |
| GTCTTGGTCATGATCACTTG+TGG | + | chr7_3:87251006-87251025 | None:intergenic | 45.0% | |
| ! | TTGTAGGTTGGACAGTGTCT+TGG | + | chr7_3:87251022-87251041 | None:intergenic | 45.0% |
| GATGGTGATTGGATGCTAGT+TGG | - | chr7_3:87251418-87251437 | Msa1133630:CDS | 45.0% | |
| TGCTAGTTGGAGATGTTCCA+TGG | - | chr7_3:87251431-87251450 | Msa1133630:intron | 45.0% | |
| AGGATCAGAAGCAAAAGGGT+TGG | - | chr7_3:87251591-87251610 | Msa1133630:CDS | 45.0% | |
| GAGCAGAGAGAGTTCTATAG+AGG | - | chr7_3:87251679-87251698 | Msa1133630:CDS | 45.0% | |
| CAGAGAGAGTTCTATAGAGG+TGG | - | chr7_3:87251682-87251701 | Msa1133630:CDS | 45.0% | |
| ! | GTTCTATAGAGGTGGCTTTG+AGG | - | chr7_3:87251690-87251709 | Msa1133630:CDS | 45.0% |
| !!! | CTTTTAATTTTAATTATTTT+TGG | - | chr7_3:87251481-87251500 | Msa1133630:intron | 5.0% |
| GCAGGGTACAAGTAGTTGGA+TGG | - | chr7_3:87251152-87251171 | Msa1133630:CDS | 50.0% | |
| TGGCTGGTGCACCTTACTTG+AGG | - | chr7_3:87251257-87251276 | Msa1133630:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 87250617 | 87251984 | 87250617 | ID=Msa1133630;Name=Msa1133630 |
| chr7_3 | mRNA | 87250617 | 87251984 | 87250617 | ID=Msa1133630-mRNA-1;Parent=Msa1133630;Name=Msa1133630-mRNA-1;_AED=0.02;_eAED=0.02;_QI=190|1|1|1|1|1|2|525|179 |
| chr7_3 | exon | 87250617 | 87251445 | 87250617 | ID=Msa1133630-mRNA-1:exon:26227;Parent=Msa1133630-mRNA-1 |
| chr7_3 | exon | 87251559 | 87251984 | 87251559 | ID=Msa1133630-mRNA-1:exon:26226;Parent=Msa1133630-mRNA-1 |
| chr7_3 | five_prime_UTR | 87251795 | 87251984 | 87251795 | ID=Msa1133630-mRNA-1:five_prime_utr;Parent=Msa1133630-mRNA-1 |
| chr7_3 | CDS | 87251559 | 87251794 | 87251559 | ID=Msa1133630-mRNA-1:cds;Parent=Msa1133630-mRNA-1 |
| chr7_3 | CDS | 87251142 | 87251445 | 87251142 | ID=Msa1133630-mRNA-1:cds;Parent=Msa1133630-mRNA-1 |
| chr7_3 | three_prime_UTR | 87250617 | 87251141 | 87250617 | ID=Msa1133630-mRNA-1:three_prime_utr;Parent=Msa1133630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
