Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0625790 | XP_003604244.1 | 100.000 | 259 | 0 | 0 | 1 | 259 | 1 | 259 | 0.0 | 524 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0625790 | sp|Q9XGM1|VATD_ARATH | 79.389 | 262 | 50 | 2 | 1 | 259 | 1 | 261 | 1.51e-151 | 426 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0625790 | G7JEF1 | 100.000 | 259 | 0 | 0 | 1 | 259 | 1 | 259 | 0.0 | 524 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000940 | Msa0625790 | 0.807801 | 4.231844e-50 | -8.615850e-47 |
| Msa0008840 | Msa0625790 | 0.818832 | 1.595150e-52 | -8.615850e-47 |
| Msa0023440 | Msa0625790 | 0.800672 | 1.291268e-48 | -8.615850e-47 |
| Msa0026660 | Msa0625790 | 0.807469 | 4.977827e-50 | -8.615850e-47 |
| Msa0027420 | Msa0625790 | 0.804512 | 2.084641e-49 | -8.615850e-47 |
| Msa0027580 | Msa0625790 | 0.807651 | 4.555575e-50 | -8.615850e-47 |
| Msa0036370 | Msa0625790 | 0.820083 | 8.273069e-53 | -8.615850e-47 |
| Msa0047980 | Msa0625790 | 0.812054 | 5.142482e-51 | -8.615850e-47 |
| Msa0055100 | Msa0625790 | 0.812092 | 5.045863e-51 | -8.615850e-47 |
| Msa0061970 | Msa0625790 | 0.800568 | 1.356230e-48 | -8.615850e-47 |
| Msa0065010 | Msa0625790 | 0.805839 | 1.099547e-49 | -8.615850e-47 |
| Msa0072170 | Msa0625790 | 0.834719 | 2.547482e-56 | -8.615850e-47 |
| Msa0073670 | Msa0625790 | 0.802105 | 6.568979e-49 | -8.615850e-47 |
| Msa0075340 | Msa0625790 | 0.806789 | 6.934869e-50 | -8.615850e-47 |
| Msa0077540 | Msa0625790 | 0.800342 | 1.507521e-48 | -8.615850e-47 |
| Msa0090030 | Msa0625790 | 0.812280 | 4.589027e-51 | -8.615850e-47 |
| Msa0094010 | Msa0625790 | 0.807294 | 5.422017e-50 | -8.615850e-47 |
| Msa0100800 | Msa0625790 | 0.811885 | 5.596640e-51 | -8.615850e-47 |
| Msa0101180 | Msa0625790 | 0.838368 | 2.998477e-57 | -8.615850e-47 |
| Msa0120540 | Msa0625790 | 0.829322 | 5.492018e-55 | -8.615850e-47 |
| Msa0607290 | Msa0625790 | -0.819302 | 1.247360e-52 | -8.615850e-47 |
| Msa0607510 | Msa0625790 | 0.800239 | 1.582283e-48 | -8.615850e-47 |
| Msa0609220 | Msa0625790 | 0.818721 | 1.690549e-52 | -8.615850e-47 |
| Msa0609970 | Msa0625790 | 0.807623 | 4.617716e-50 | -8.615850e-47 |
| Msa0615660 | Msa0625790 | 0.814673 | 1.366456e-51 | -8.615850e-47 |
| Msa0617260 | Msa0625790 | 0.809316 | 2.009357e-50 | -8.615850e-47 |
| Msa0625790 | Msa0626940 | 0.810540 | 1.095704e-50 | -8.615850e-47 |
| Msa0625790 | Msa0633760 | 0.800361 | 1.494409e-48 | -8.615850e-47 |
| Msa0625790 | Msa0639120 | 0.823959 | 1.045643e-53 | -8.615850e-47 |
| Msa0625790 | Msa0649410 | 0.802118 | 6.529292e-49 | -8.615850e-47 |
| Msa0625790 | Msa0650190 | 0.801767 | 7.710190e-49 | -8.615850e-47 |
| Msa0625790 | Msa0663510 | 0.810826 | 9.503061e-51 | -8.615850e-47 |
| Msa0625790 | Msa0664760 | 0.806608 | 7.575615e-50 | -8.615850e-47 |
| Msa0625790 | Msa0664960 | 0.809750 | 1.621396e-50 | -8.615850e-47 |
| Msa0625790 | Msa0687230 | 0.803367 | 3.605596e-49 | -8.615850e-47 |
| Msa0625790 | Msa0692110 | 0.810270 | 1.252772e-50 | -8.615850e-47 |
| Msa0625790 | Msa0704800 | 0.805439 | 1.334177e-49 | -8.615850e-47 |
| Msa0625790 | Msa0707390 | 0.800671 | 1.292032e-48 | -8.615850e-47 |
| Msa0625790 | Msa0711490 | 0.861283 | 1.143542e-63 | -8.615850e-47 |
| Msa0625790 | Msa0713460 | 0.800361 | 1.494227e-48 | -8.615850e-47 |
| Msa0625790 | Msa0716860 | 0.815512 | 8.899541e-52 | -8.615850e-47 |
| Msa0625790 | Msa0720370 | 0.801096 | 1.057937e-48 | -8.615850e-47 |
| Msa0625790 | Msa0748920 | 0.805363 | 1.383758e-49 | -8.615850e-47 |
| Msa0625790 | Msa0751010 | 0.824852 | 6.446774e-54 | -8.615850e-47 |
| Msa0625790 | Msa0753770 | 0.837824 | 4.137971e-57 | -8.615850e-47 |
| Msa0625790 | Msa0792090 | 0.803387 | 3.571103e-49 | -8.615850e-47 |
| Msa0625790 | Msa0793720 | 0.802874 | 4.561463e-49 | -8.615850e-47 |
| Msa0625790 | Msa0794050 | 0.844238 | 8.556345e-59 | -8.615850e-47 |
| Msa0625790 | Msa0795660 | 0.803114 | 4.068784e-49 | -8.615850e-47 |
| Msa0625790 | Msa0797750 | 0.810521 | 1.106049e-50 | -8.615850e-47 |
| Msa0625790 | Msa0798810 | -0.816337 | 5.824878e-52 | -8.615850e-47 |
| Msa0625790 | Msa0814250 | 0.804174 | 2.452011e-49 | -8.615850e-47 |
| Msa0625790 | Msa0830990 | 0.801450 | 8.953469e-49 | -8.615850e-47 |
| Msa0625790 | Msa0869010 | 0.818380 | 2.020776e-52 | -8.615850e-47 |
| Msa0625790 | Msa0888940 | 0.800701 | 1.273817e-48 | -8.615850e-47 |
| Msa0625790 | Msa0901110 | 0.808917 | 2.446293e-50 | -8.615850e-47 |
| Msa0625790 | Msa0905380 | 0.802671 | 5.023111e-49 | -8.615850e-47 |
| Msa0625790 | Msa0921870 | 0.802006 | 6.886111e-49 | -8.615850e-47 |
| Msa0625790 | Msa0928120 | 0.805677 | 1.189251e-49 | -8.615850e-47 |
| Msa0625790 | Msa0937330 | 0.815982 | 6.991912e-52 | -8.615850e-47 |
| Msa0625790 | Msa0940830 | 0.818222 | 2.194037e-52 | -8.615850e-47 |
| Msa0625790 | Msa0957450 | -0.802060 | 6.710886e-49 | -8.615850e-47 |
| Msa0625790 | Msa0959010 | 0.827296 | 1.692270e-54 | -8.615850e-47 |
| Msa0625790 | Msa0959840 | 0.801649 | 8.151930e-49 | -8.615850e-47 |
| Msa0625790 | Msa0976330 | 0.801375 | 9.276676e-49 | -8.615850e-47 |
| Msa0625790 | Msa0993530 | 0.816711 | 4.802999e-52 | -8.615850e-47 |
| Msa0625790 | Msa1000930 | 0.811635 | 6.342406e-51 | -8.615850e-47 |
| Msa0625790 | Msa1004420 | 0.802657 | 5.055135e-49 | -8.615850e-47 |
| Msa0625790 | Msa1009780 | 0.805530 | 1.276876e-49 | -8.615850e-47 |
| Msa0625790 | Msa1010460 | 0.802091 | 6.611583e-49 | -8.615850e-47 |
| Msa0625790 | Msa1027360 | 0.801392 | 9.199470e-49 | -8.615850e-47 |
| Msa0625790 | Msa1033520 | 0.802167 | 6.379141e-49 | -8.615850e-47 |
| Msa0625790 | Msa1036740 | 0.804343 | 2.260513e-49 | -8.615850e-47 |
| Msa0625790 | Msa1073400 | 0.836773 | 7.687555e-57 | -8.615850e-47 |
| Msa0625790 | Msa1113590 | 0.802114 | 6.540523e-49 | -8.615850e-47 |
| Msa0625790 | Msa1113880 | 0.858293 | 9.096792e-63 | -8.615850e-47 |
| Msa0625790 | Msa1132310 | 0.816753 | 4.701024e-52 | -8.615850e-47 |
| Msa0625790 | Msa1133630 | 0.812426 | 4.265544e-51 | -8.615850e-47 |
| Msa0625790 | Msa1144970 | 0.808389 | 3.172926e-50 | -8.615850e-47 |
| Msa0625790 | Msa1160360 | 0.818944 | 1.504489e-52 | -8.615850e-47 |
| Msa0625790 | Msa1171770 | -0.802501 | 5.445428e-49 | -8.615850e-47 |
| Msa0625790 | Msa1182770 | 0.802598 | 5.199470e-49 | -8.615850e-47 |
| Msa0625790 | Msa1185130 | 0.815136 | 1.078997e-51 | -8.615850e-47 |
| Msa0625790 | Msa1188560 | 0.807510 | 4.880772e-50 | -8.615850e-47 |
| Msa0625790 | Msa1192850 | 0.804744 | 1.864884e-49 | -8.615850e-47 |
| Msa0625790 | Msa1199290 | 0.813553 | 2.414934e-51 | -8.615850e-47 |
| Msa0625790 | Msa1201810 | 0.800981 | 1.116884e-48 | -8.615850e-47 |
| Msa0625790 | Msa1201830 | 0.814912 | 1.209526e-51 | -8.615850e-47 |
| Msa0625790 | Msa1204730 | 0.822973 | 1.778740e-53 | -8.615850e-47 |
| Msa0625790 | Msa1205480 | 0.832534 | 8.950016e-56 | -8.615850e-47 |
| Msa0625790 | Msa1205590 | 0.806467 | 8.111561e-50 | -8.615850e-47 |
| Msa0625790 | Msa1207730 | 0.819823 | 9.486312e-53 | -8.615850e-47 |
| Msa0625790 | Msa1214320 | 0.811544 | 6.640419e-51 | -8.615850e-47 |
| Msa0625790 | Msa1235630 | 0.814417 | 1.556874e-51 | -8.615850e-47 |
| Msa0625790 | Msa1253810 | 0.838591 | 2.625905e-57 | -8.615850e-47 |
| Msa0625790 | Msa1261070 | 0.803968 | 2.705893e-49 | -8.615850e-47 |
| Msa0625790 | Msa1271580 | 0.811355 | 7.298427e-51 | -8.615850e-47 |
| Msa0625790 | Msa1283990 | 0.806578 | 7.686621e-50 | -8.615850e-47 |
| Msa0625790 | Msa1286510 | 0.806430 | 8.256628e-50 | -8.615850e-47 |
| Msa0625790 | Msa1296300 | 0.835459 | 1.657152e-56 | -8.615850e-47 |
| Msa0625790 | Msa1299360 | 0.830856 | 2.320165e-55 | -8.615850e-47 |
| Msa0625790 | Msa1299700 | 0.810108 | 1.357993e-50 | -8.615850e-47 |
| Msa0625790 | Msa1310690 | 0.801983 | 6.959411e-49 | -8.615850e-47 |
| Msa0625790 | Msa1327490 | 0.811430 | 7.028373e-51 | -8.615850e-47 |
| Msa0625790 | Msa1338610 | 0.814053 | 1.874309e-51 | -8.615850e-47 |
| Msa0625790 | Msa1363700 | 0.814160 | 1.775068e-51 | -8.615850e-47 |
| Msa0625790 | Msa1372310 | 0.809622 | 1.727830e-50 | -8.615850e-47 |
| Msa0625790 | Msa1373880 | 0.812808 | 3.519464e-51 | -8.615850e-47 |
| Msa0625790 | Msa1381650 | 0.814481 | 1.507455e-51 | -8.615850e-47 |
| Msa0625790 | Msa1382410 | 0.809318 | 2.007454e-50 | -8.615850e-47 |
| Msa0625790 | Msa1385350 | 0.833626 | 4.786347e-56 | -8.615850e-47 |
| Msa0625790 | Msa1393800 | 0.827103 | 1.881966e-54 | -8.615850e-47 |
| Msa0625790 | Msa1396940 | 0.819618 | 1.056680e-52 | -8.615850e-47 |
| Msa0625790 | Msa1397940 | 0.810451 | 1.145398e-50 | -8.615850e-47 |
| Msa0625790 | Msa1426910 | 0.835287 | 1.832105e-56 | -8.615850e-47 |
| Msa0625790 | Msa1431040 | 0.819191 | 1.322004e-52 | -8.615850e-47 |
| Msa0625790 | Msa1433600 | 0.813869 | 2.057724e-51 | -8.615850e-47 |
| Msa0625790 | Msa1438450 | 0.825423 | 4.726037e-54 | -8.615850e-47 |
| Msa0625790 | Msa1450870 | 0.804559 | 2.038509e-49 | -8.615850e-47 |
| Msa0625790 | Msa1452470 | 0.800392 | 1.472329e-48 | -8.615850e-47 |
| Msa0127040 | Msa0625790 | 0.809749 | 1.622407e-50 | -8.615850e-47 |
| Msa0145150 | Msa0625790 | 0.833179 | 6.188803e-56 | -8.615850e-47 |
| Msa0152840 | Msa0625790 | 0.801814 | 7.537721e-49 | -8.615850e-47 |
| Msa0166750 | Msa0625790 | 0.807542 | 4.803293e-50 | -8.615850e-47 |
| Msa0174000 | Msa0625790 | 0.807410 | 5.123842e-50 | -8.615850e-47 |
| Msa0182280 | Msa0625790 | 0.805609 | 1.229216e-49 | -8.615850e-47 |
| Msa0183460 | Msa0625790 | 0.809205 | 2.123052e-50 | -8.615850e-47 |
| Msa0212750 | Msa0625790 | -0.834088 | 3.668658e-56 | -8.615850e-47 |
| Msa0236270 | Msa0625790 | 0.803610 | 3.210610e-49 | -8.615850e-47 |
| Msa0236980 | Msa0625790 | 0.805165 | 1.522458e-49 | -8.615850e-47 |
| Msa0392940 | Msa0625790 | 0.802269 | 6.078103e-49 | -8.615850e-47 |
| Msa0394400 | Msa0625790 | 0.809622 | 1.727555e-50 | -8.615850e-47 |
| Msa0431770 | Msa0625790 | 0.822242 | 2.630203e-53 | -8.615850e-47 |
| Msa0432070 | Msa0625790 | 0.827083 | 1.902978e-54 | -8.615850e-47 |
| Msa0436190 | Msa0625790 | 0.808565 | 2.909582e-50 | -8.615850e-47 |
| Msa0436200 | Msa0625790 | 0.810728 | 9.979999e-51 | -8.615850e-47 |
| Msa0437510 | Msa0625790 | 0.805486 | 1.304059e-49 | -8.615850e-47 |
| Msa0442270 | Msa0625790 | 0.807143 | 5.836833e-50 | -8.615850e-47 |
| Msa0442310 | Msa0625790 | 0.804734 | 1.874037e-49 | -8.615850e-47 |
| Msa0449820 | Msa0625790 | 0.815118 | 1.088948e-51 | -8.615850e-47 |
| Msa0465960 | Msa0625790 | 0.805624 | 1.220194e-49 | -8.615850e-47 |
| Msa0241740 | Msa0625790 | 0.830036 | 3.681014e-55 | -8.615850e-47 |
| Msa0265160 | Msa0625790 | 0.806993 | 6.279121e-50 | -8.615850e-47 |
| Msa0281360 | Msa0625790 | 0.821487 | 3.933527e-53 | -8.615850e-47 |
| Msa0310410 | Msa0625790 | 0.814211 | 1.729073e-51 | -8.615850e-47 |
| Msa0328210 | Msa0625790 | 0.822454 | 2.348633e-53 | -8.615850e-47 |
| Msa0337510 | Msa0625790 | 0.808692 | 2.733342e-50 | -8.615850e-47 |
| Msa0358510 | Msa0625790 | 0.808394 | 3.164754e-50 | -8.615850e-47 |
| Msa0495220 | Msa0625790 | 0.808507 | 2.993635e-50 | -8.615850e-47 |
| Msa0510290 | Msa0625790 | 0.808777 | 2.621945e-50 | -8.615850e-47 |
| Msa0524590 | Msa0625790 | 0.800903 | 1.158701e-48 | -8.615850e-47 |
| Msa0534080 | Msa0625790 | 0.819701 | 1.011502e-52 | -8.615850e-47 |
| Msa0534410 | Msa0625790 | 0.812764 | 3.597013e-51 | -8.615850e-47 |
| Msa0560470 | Msa0625790 | 0.820857 | 5.494434e-53 | -8.615850e-47 |
| Msa0578090 | Msa0625790 | 0.821817 | 3.299955e-53 | -8.615850e-47 |
| Msa0579370 | Msa0625790 | 0.814641 | 1.389370e-51 | -8.615850e-47 |
| Msa0582280 | Msa0625790 | 0.807932 | 3.970508e-50 | -8.615850e-47 |
| Msa0585900 | Msa0625790 | 0.833658 | 4.698262e-56 | -8.615850e-47 |
| Msa0586850 | Msa0625790 | 0.812016 | 5.241774e-51 | -8.615850e-47 |
| Msa0588120 | Msa0625790 | 0.805935 | 1.049610e-49 | -8.615850e-47 |
| Msa0590650 | Msa0625790 | 0.804067 | 2.581226e-49 | -8.615850e-47 |
| Msa0591540 | Msa0625790 | 0.827786 | 1.290592e-54 | -8.615850e-47 |
| Msa0599900 | Msa0625790 | 0.806102 | 9.684028e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0625790 | MtrunA17_Chr4g0001341 | 100.000 | 259 | 0 | 0 | 1 | 259 | 1 | 259 | 0.0 | 524 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0625790 | AT3G58730.1 | 79.389 | 262 | 50 | 2 | 1 | 259 | 1 | 261 | 1.53e-152 | 426 |
Find 64 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTAGGTTGAAGAAGATTC+AGG | 0.251278 | 4_3:-5144773 | Msa0625790:CDS |
| GAATACTATTAGTTACATTA+AGG | 0.278798 | 4_3:-5144827 | Msa0625790:CDS |
| GTGTCGTGTTGCTTATATTA+AGG | 0.324047 | 4_3:-5144971 | Msa0625790:CDS |
| TTGAAAGGGAGGATTTCTTT+AGG | 0.370172 | 4_3:-5144789 | Msa0625790:CDS |
| AACTTGTTGGCTGGTACGAC+TGG | 0.385007 | 4_3:-5144646 | Msa0625790:CDS |
| GACAATGCTTGGAGTGGTTA+AGG | 0.388176 | 4_3:-5145337 | Msa0625790:CDS |
| TGAAACCTCTCACAACTTGT+TGG | 0.400530 | 4_3:-5144659 | Msa0625790:CDS |
| AGAAGATTCAGGGTTATAAG+AGG | 0.430053 | 4_3:-5144762 | Msa0625790:CDS |
| TGTTTCAACAAAGGAATCAA+TGG | 0.435503 | 4_3:-5145223 | Msa0625790:CDS |
| GTGAGTTGGATGAGCTTGAA+AGG | 0.439414 | 4_3:-5144804 | Msa0625790:CDS |
| AATACTATTAGTTACATTAA+GGG | 0.450457 | 4_3:-5144826 | Msa0625790:CDS |
| TAGTTACATTAAGGGTGAGT+TGG | 0.451344 | 4_3:-5144818 | Msa0625790:CDS |
| AAAGGAAGATGGTGATTGCT+AGG | 0.464882 | 4_3:-5144729 | Msa0625790:CDS |
| TGGAGTGGTTAAGGCACGTT+TGG | 0.467712 | 4_3:-5145328 | Msa0625790:CDS |
| GTTTCAACAAAGGAATCAAT+GGG | 0.471657 | 4_3:-5145222 | Msa0625790:CDS |
| ACCTCTCACAACTTGTTGGC+TGG | 0.473238 | 4_3:-5144655 | Msa0625790:CDS |
| TGCTTATATTAAGGCGATTG+AGG | 0.474951 | 4_3:-5144962 | Msa0625790:CDS |
| AAAAGTTACAATTGCAGCAA+GGG | 0.488148 | 4_3:-5144690 | Msa0625790:CDS |
| CTAATAGTATTCTCCAACCT+CGG | 0.495607 | 4_3:+5144838 | None:intergenic |
| GTTGGATGAGCTTGAAAGGG+AGG | 0.499311 | 4_3:-5144800 | Msa0625790:CDS |
| GTGGTTAAGGCACGTTTGGT+TGG | 0.501885 | 4_3:-5145324 | Msa0625790:CDS |
| TTTAGGTTGAAGAAGATTCA+GGG | 0.502528 | 4_3:-5144772 | Msa0625790:CDS |
| TAATAGCATCATCAAGGGTA+AGG | 0.507164 | 4_3:+5144905 | None:intergenic |
| TGAGTTGGATGAGCTTGAAA+GGG | 0.514528 | 4_3:-5144803 | Msa0625790:CDS |
| ATTGGCTAGAGGTGGTCAGC+AGG | 0.515670 | 4_3:-5145001 | Msa0625790:CDS |
| GATTCAGGGTTATAAGAGGA+GGG | 0.517039 | 4_3:-5144758 | Msa0625790:CDS |
| TCCAGTCAGATCATTCTTCG+TGG | 0.520047 | 4_3:+5145023 | None:intergenic |
| TCAGCACTGTAATCAAACTT+TGG | 0.538953 | 4_3:+5145054 | None:intergenic |
| TAGTCTTAATAGCATCATCA+AGG | 0.542669 | 4_3:+5144899 | None:intergenic |
| GAAAAGTTACAATTGCAGCA+AGG | 0.543936 | 4_3:-5144691 | Msa0625790:CDS |
| AGATTCAGGGTTATAAGAGG+AGG | 0.545100 | 4_3:-5144759 | Msa0625790:CDS |
| GAAGAATGATCTGACTGGAT+TGG | 0.545863 | 4_3:-5145019 | Msa0625790:CDS |
| GAGGGAAATTGAAAGGAAGA+TGG | 0.546430 | 4_3:-5144740 | Msa0625790:CDS |
| GAATGTTGTTAAGCCGAGGT+TGG | 0.546577 | 4_3:-5144851 | Msa0625790:CDS |
| GTCCCCACAGTGACAATGCT+TGG | 0.555756 | 4_3:-5145348 | Msa0625790:CDS |
| ATCATCAAGGGTAAGGAAAG+AGG | 0.556678 | 4_3:+5144912 | None:intergenic |
| ATAAGAGGAGGGAAATTGAA+AGG | 0.563654 | 4_3:-5144747 | Msa0625790:CDS |
| TCTCGTACTGAGAATGTGGC+AGG | 0.566710 | 4_3:-5145087 | Msa0625790:CDS |
| ATGTTAAAGAAGCTTCACTC+AGG | 0.568588 | 4_3:-5145116 | Msa0625790:CDS |
| CTATTAAGACTACTAACCGT+AGG | 0.570720 | 4_3:-5144888 | Msa0625790:CDS |
| GCCACGAAGAATGATCTGAC+TGG | 0.577877 | 4_3:-5145024 | Msa0625790:CDS |
| ACTCCAAGCATTGTCACTGT+GGG | 0.583853 | 4_3:+5145345 | None:intergenic |
| ACTGAGGCTAAGTATGTAGC+CGG | 0.591330 | 4_3:-5145168 | Msa0625790:CDS |
| GCAATCACAACGTTTGAATG+TGG | 0.591655 | 4_3:-5145370 | Msa0625790:CDS |
| TTCTTCATTTGCATTAACTG+AGG | 0.593026 | 4_3:-5145184 | Msa0625790:CDS |
| CGTTTGGTTGGAGCAACAAG+AGG | 0.602491 | 4_3:-5145312 | Msa0625790:CDS |
| TTGAGAATGTTGTTAAGCCG+AGG | 0.609957 | 4_3:-5144855 | Msa0625790:CDS |
| AAGGAAGATGGTGATTGCTA+GGG | 0.610095 | 4_3:-5144728 | Msa0625790:CDS |
| CACTCCAAGCATTGTCACTG+TGG | 0.610571 | 4_3:+5145344 | None:intergenic |
| AAGTTTGATTACAGTGCTGA+CGG | 0.611337 | 4_3:-5145051 | Msa0625790:CDS |
| TCTCAAGAGCATTAACCCTA+CGG | 0.615251 | 4_3:+5144872 | None:intergenic |
| AAAGTTACAATTGCAGCAAG+GGG | 0.616721 | 4_3:-5144689 | Msa0625790:CDS |
| AGTCTTAATAGCATCATCAA+GGG | 0.622324 | 4_3:+5144900 | None:intergenic |
| GACGTGCTTGATGTTATCAC+CGG | 0.624967 | 4_3:+5145149 | None:intergenic |
| ACGTTCTCGTACTGAGAATG+TGG | 0.627068 | 4_3:-5145091 | Msa0625790:CDS |
| CACAGTGACAATGCTTGGAG+TGG | 0.627138 | 4_3:-5145343 | Msa0625790:CDS |
| TATTAAGACTACTAACCGTA+GGG | 0.628796 | 4_3:-5144887 | Msa0625790:CDS |
| ACCAGCCAACAAGTTGTGAG+AGG | 0.637543 | 4_3:+5144654 | None:intergenic |
| TGTTAAAGAAGCTTCACTCA+GGG | 0.649586 | 4_3:-5145115 | Msa0625790:CDS |
| CTGACTGGATTGGCTAGAGG+TGG | 0.655401 | 4_3:-5145009 | Msa0625790:CDS |
| TGATTACAGTGCTGACGGTG+AGG | 0.656119 | 4_3:-5145046 | Msa0625790:CDS |
| GATCTGACTGGATTGGCTAG+AGG | 0.670803 | 4_3:-5145012 | Msa0625790:CDS |
| CTCCAAGCATTGTCACTGTG+GGG | 0.709998 | 4_3:+5145346 | None:intergenic |
| TGGTACGACTGGAAAAGACG+AGG | 0.748180 | 4_3:-5144635 | Msa0625790:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATACTATTAGTTACATTAA+GGG | - | chr4_3:5145173-5145192 | Msa0625790:CDS | 15.0% |
| !! | AATACTATTAGTTACATTAA+GGG | - | chr4_3:5145173-5145192 | Msa0625790:CDS | 15.0% |
| !! | GAATACTATTAGTTACATTA+AGG | - | chr4_3:5145172-5145191 | Msa0625790:CDS | 20.0% |
| !! | GAATACTATTAGTTACATTA+AGG | - | chr4_3:5145172-5145191 | Msa0625790:CDS | 20.0% |
| ! | GAAAAAGATTGTTTCAACAA+AGG | - | chr4_3:5144767-5144786 | Msa0625790:CDS | 25.0% |
| ! | GAAAAAGATTGTTTCAACAA+AGG | - | chr4_3:5144767-5144786 | Msa0625790:CDS | 25.0% |
| TGTTTCAACAAAGGAATCAA+TGG | - | chr4_3:5144776-5144795 | Msa0625790:CDS | 30.0% | |
| GTTTCAACAAAGGAATCAAT+GGG | - | chr4_3:5144777-5144796 | Msa0625790:CDS | 30.0% | |
| TTCTTCATTTGCATTAACTG+AGG | - | chr4_3:5144815-5144834 | Msa0625790:CDS | 30.0% | |
| AGTCTTAATAGCATCATCAA+GGG | + | chr4_3:5145102-5145121 | None:intergenic | 30.0% | |
| ! | TAGTCTTAATAGCATCATCA+AGG | + | chr4_3:5145103-5145122 | None:intergenic | 30.0% |
| TATTAAGACTACTAACCGTA+GGG | - | chr4_3:5145112-5145131 | Msa0625790:CDS | 30.0% | |
| !! | TTTAGGTTGAAGAAGATTCA+GGG | - | chr4_3:5145227-5145246 | Msa0625790:CDS | 30.0% |
| AAAAGTTACAATTGCAGCAA+GGG | - | chr4_3:5145309-5145328 | Msa0625790:CDS | 30.0% | |
| TGTTTCAACAAAGGAATCAA+TGG | - | chr4_3:5144776-5144795 | Msa0625790:CDS | 30.0% | |
| GTTTCAACAAAGGAATCAAT+GGG | - | chr4_3:5144777-5144796 | Msa0625790:CDS | 30.0% | |
| TTCTTCATTTGCATTAACTG+AGG | - | chr4_3:5144815-5144834 | Msa0625790:CDS | 30.0% | |
| AGTCTTAATAGCATCATCAA+GGG | + | chr4_3:5145102-5145121 | None:intergenic | 30.0% | |
| ! | TAGTCTTAATAGCATCATCA+AGG | + | chr4_3:5145103-5145122 | None:intergenic | 30.0% |
| TATTAAGACTACTAACCGTA+GGG | - | chr4_3:5145112-5145131 | Msa0625790:CDS | 30.0% | |
| !! | TTTAGGTTGAAGAAGATTCA+GGG | - | chr4_3:5145227-5145246 | Msa0625790:CDS | 30.0% |
| AAAAGTTACAATTGCAGCAA+GGG | - | chr4_3:5145309-5145328 | Msa0625790:CDS | 30.0% | |
| ATGTTAAAGAAGCTTCACTC+AGG | - | chr4_3:5144883-5144902 | Msa0625790:CDS | 35.0% | |
| TGTTAAAGAAGCTTCACTCA+GGG | - | chr4_3:5144884-5144903 | Msa0625790:CDS | 35.0% | |
| TCAGCACTGTAATCAAACTT+TGG | + | chr4_3:5144948-5144967 | None:intergenic | 35.0% | |
| ! | AAGTTTGATTACAGTGCTGA+CGG | - | chr4_3:5144948-5144967 | Msa0625790:CDS | 35.0% |
| !! | GTGTCGTGTTGCTTATATTA+AGG | - | chr4_3:5145028-5145047 | Msa0625790:CDS | 35.0% |
| TGCTTATATTAAGGCGATTG+AGG | - | chr4_3:5145037-5145056 | Msa0625790:CDS | 35.0% | |
| TAATAGCATCATCAAGGGTA+AGG | + | chr4_3:5145097-5145116 | None:intergenic | 35.0% | |
| CTATTAAGACTACTAACCGT+AGG | - | chr4_3:5145111-5145130 | Msa0625790:CDS | 35.0% | |
| CTAATAGTATTCTCCAACCT+CGG | + | chr4_3:5145164-5145183 | None:intergenic | 35.0% | |
| TAGTTACATTAAGGGTGAGT+TGG | - | chr4_3:5145181-5145200 | Msa0625790:CDS | 35.0% | |
| TTGAAAGGGAGGATTTCTTT+AGG | - | chr4_3:5145210-5145229 | Msa0625790:CDS | 35.0% | |
| !! | CTTTAGGTTGAAGAAGATTC+AGG | - | chr4_3:5145226-5145245 | Msa0625790:CDS | 35.0% |
| AGAAGATTCAGGGTTATAAG+AGG | - | chr4_3:5145237-5145256 | Msa0625790:CDS | 35.0% | |
| ATAAGAGGAGGGAAATTGAA+AGG | - | chr4_3:5145252-5145271 | Msa0625790:CDS | 35.0% | |
| GAAAAGTTACAATTGCAGCA+AGG | - | chr4_3:5145308-5145327 | Msa0625790:CDS | 35.0% | |
| AAAGTTACAATTGCAGCAAG+GGG | - | chr4_3:5145310-5145329 | Msa0625790:CDS | 35.0% | |
| ATGTTAAAGAAGCTTCACTC+AGG | - | chr4_3:5144883-5144902 | Msa0625790:CDS | 35.0% | |
| TGTTAAAGAAGCTTCACTCA+GGG | - | chr4_3:5144884-5144903 | Msa0625790:CDS | 35.0% | |
| TCAGCACTGTAATCAAACTT+TGG | + | chr4_3:5144948-5144967 | None:intergenic | 35.0% | |
| ! | AAGTTTGATTACAGTGCTGA+CGG | - | chr4_3:5144948-5144967 | Msa0625790:CDS | 35.0% |
| !! | GTGTCGTGTTGCTTATATTA+AGG | - | chr4_3:5145028-5145047 | Msa0625790:CDS | 35.0% |
| TGCTTATATTAAGGCGATTG+AGG | - | chr4_3:5145037-5145056 | Msa0625790:CDS | 35.0% | |
| TAATAGCATCATCAAGGGTA+AGG | + | chr4_3:5145097-5145116 | None:intergenic | 35.0% | |
| CTATTAAGACTACTAACCGT+AGG | - | chr4_3:5145111-5145130 | Msa0625790:CDS | 35.0% | |
| CTAATAGTATTCTCCAACCT+CGG | + | chr4_3:5145164-5145183 | None:intergenic | 35.0% | |
| TAGTTACATTAAGGGTGAGT+TGG | - | chr4_3:5145181-5145200 | Msa0625790:CDS | 35.0% | |
| TTGAAAGGGAGGATTTCTTT+AGG | - | chr4_3:5145210-5145229 | Msa0625790:CDS | 35.0% | |
| !! | CTTTAGGTTGAAGAAGATTC+AGG | - | chr4_3:5145226-5145245 | Msa0625790:CDS | 35.0% |
| AGAAGATTCAGGGTTATAAG+AGG | - | chr4_3:5145237-5145256 | Msa0625790:CDS | 35.0% | |
| ATAAGAGGAGGGAAATTGAA+AGG | - | chr4_3:5145252-5145271 | Msa0625790:CDS | 35.0% | |
| GAAAAGTTACAATTGCAGCA+AGG | - | chr4_3:5145308-5145327 | Msa0625790:CDS | 35.0% | |
| AAAGTTACAATTGCAGCAAG+GGG | - | chr4_3:5145310-5145329 | Msa0625790:CDS | 35.0% | |
| GCAATCACAACGTTTGAATG+TGG | - | chr4_3:5144629-5144648 | Msa0625790:CDS | 40.0% | |
| GAAGAATGATCTGACTGGAT+TGG | - | chr4_3:5144980-5144999 | Msa0625790:CDS | 40.0% | |
| ATCATCAAGGGTAAGGAAAG+AGG | + | chr4_3:5145090-5145109 | None:intergenic | 40.0% | |
| !! | TCTCAAGAGCATTAACCCTA+CGG | + | chr4_3:5145130-5145149 | None:intergenic | 40.0% |
| TTGAGAATGTTGTTAAGCCG+AGG | - | chr4_3:5145144-5145163 | Msa0625790:CDS | 40.0% | |
| ! | TGAGTTGGATGAGCTTGAAA+GGG | - | chr4_3:5145196-5145215 | Msa0625790:CDS | 40.0% |
| AGATTCAGGGTTATAAGAGG+AGG | - | chr4_3:5145240-5145259 | Msa0625790:CDS | 40.0% | |
| GATTCAGGGTTATAAGAGGA+GGG | - | chr4_3:5145241-5145260 | Msa0625790:CDS | 40.0% | |
| GAGGGAAATTGAAAGGAAGA+TGG | - | chr4_3:5145259-5145278 | Msa0625790:CDS | 40.0% | |
| AAAGGAAGATGGTGATTGCT+AGG | - | chr4_3:5145270-5145289 | Msa0625790:CDS | 40.0% | |
| AAGGAAGATGGTGATTGCTA+GGG | - | chr4_3:5145271-5145290 | Msa0625790:CDS | 40.0% | |
| TGAAACCTCTCACAACTTGT+TGG | - | chr4_3:5145340-5145359 | Msa0625790:CDS | 40.0% | |
| GCAATCACAACGTTTGAATG+TGG | - | chr4_3:5144629-5144648 | Msa0625790:CDS | 40.0% | |
| GAAGAATGATCTGACTGGAT+TGG | - | chr4_3:5144980-5144999 | Msa0625790:CDS | 40.0% | |
| ATCATCAAGGGTAAGGAAAG+AGG | + | chr4_3:5145090-5145109 | None:intergenic | 40.0% | |
| !! | TCTCAAGAGCATTAACCCTA+CGG | + | chr4_3:5145130-5145149 | None:intergenic | 40.0% |
| TTGAGAATGTTGTTAAGCCG+AGG | - | chr4_3:5145144-5145163 | Msa0625790:CDS | 40.0% | |
| ! | TGAGTTGGATGAGCTTGAAA+GGG | - | chr4_3:5145196-5145215 | Msa0625790:CDS | 40.0% |
| AGATTCAGGGTTATAAGAGG+AGG | - | chr4_3:5145240-5145259 | Msa0625790:CDS | 40.0% | |
| GATTCAGGGTTATAAGAGGA+GGG | - | chr4_3:5145241-5145260 | Msa0625790:CDS | 40.0% | |
| GAGGGAAATTGAAAGGAAGA+TGG | - | chr4_3:5145259-5145278 | Msa0625790:CDS | 40.0% | |
| AAAGGAAGATGGTGATTGCT+AGG | - | chr4_3:5145270-5145289 | Msa0625790:CDS | 40.0% | |
| AAGGAAGATGGTGATTGCTA+GGG | - | chr4_3:5145271-5145290 | Msa0625790:CDS | 40.0% | |
| TGAAACCTCTCACAACTTGT+TGG | - | chr4_3:5145340-5145359 | Msa0625790:CDS | 40.0% | |
| ! | ACTCCAAGCATTGTCACTGT+GGG | + | chr4_3:5144657-5144676 | None:intergenic | 45.0% |
| GACAATGCTTGGAGTGGTTA+AGG | - | chr4_3:5144662-5144681 | Msa0625790:CDS | 45.0% | |
| ACTGAGGCTAAGTATGTAGC+CGG | - | chr4_3:5144831-5144850 | Msa0625790:CDS | 45.0% | |
| GACGTGCTTGATGTTATCAC+CGG | + | chr4_3:5144853-5144872 | None:intergenic | 45.0% | |
| ACGTTCTCGTACTGAGAATG+TGG | - | chr4_3:5144908-5144927 | Msa0625790:CDS | 45.0% | |
| TCCAGTCAGATCATTCTTCG+TGG | + | chr4_3:5144979-5144998 | None:intergenic | 45.0% | |
| GAATGTTGTTAAGCCGAGGT+TGG | - | chr4_3:5145148-5145167 | Msa0625790:CDS | 45.0% | |
| ! | GTGAGTTGGATGAGCTTGAA+AGG | - | chr4_3:5145195-5145214 | Msa0625790:CDS | 45.0% |
| ! | ACTCCAAGCATTGTCACTGT+GGG | + | chr4_3:5144657-5144676 | None:intergenic | 45.0% |
| GACAATGCTTGGAGTGGTTA+AGG | - | chr4_3:5144662-5144681 | Msa0625790:CDS | 45.0% | |
| ACTGAGGCTAAGTATGTAGC+CGG | - | chr4_3:5144831-5144850 | Msa0625790:CDS | 45.0% | |
| GACGTGCTTGATGTTATCAC+CGG | + | chr4_3:5144853-5144872 | None:intergenic | 45.0% | |
| ACGTTCTCGTACTGAGAATG+TGG | - | chr4_3:5144908-5144927 | Msa0625790:CDS | 45.0% | |
| TCCAGTCAGATCATTCTTCG+TGG | + | chr4_3:5144979-5144998 | None:intergenic | 45.0% | |
| GAATGTTGTTAAGCCGAGGT+TGG | - | chr4_3:5145148-5145167 | Msa0625790:CDS | 45.0% | |
| ! | GTGAGTTGGATGAGCTTGAA+AGG | - | chr4_3:5145195-5145214 | Msa0625790:CDS | 45.0% |
| ! | CTCCAAGCATTGTCACTGTG+GGG | + | chr4_3:5144656-5144675 | None:intergenic | 50.0% |
| ! | CACTCCAAGCATTGTCACTG+TGG | + | chr4_3:5144658-5144677 | None:intergenic | 50.0% |
| CACAGTGACAATGCTTGGAG+TGG | - | chr4_3:5144656-5144675 | Msa0625790:CDS | 50.0% | |
| TGGAGTGGTTAAGGCACGTT+TGG | - | chr4_3:5144671-5144690 | Msa0625790:CDS | 50.0% | |
| GTGGTTAAGGCACGTTTGGT+TGG | - | chr4_3:5144675-5144694 | Msa0625790:CDS | 50.0% | |
| CGTTTGGTTGGAGCAACAAG+AGG | - | chr4_3:5144687-5144706 | Msa0625790:CDS | 50.0% | |
| TCTCGTACTGAGAATGTGGC+AGG | - | chr4_3:5144912-5144931 | Msa0625790:CDS | 50.0% | |
| !! | TGATTACAGTGCTGACGGTG+AGG | - | chr4_3:5144953-5144972 | Msa0625790:CDS | 50.0% |
| GCCACGAAGAATGATCTGAC+TGG | - | chr4_3:5144975-5144994 | Msa0625790:CDS | 50.0% | |
| !! | GATCTGACTGGATTGGCTAG+AGG | - | chr4_3:5144987-5145006 | Msa0625790:CDS | 50.0% |
| GTTGGATGAGCTTGAAAGGG+AGG | - | chr4_3:5145199-5145218 | Msa0625790:CDS | 50.0% | |
| !! | ACCTCTCACAACTTGTTGGC+TGG | - | chr4_3:5145344-5145363 | Msa0625790:CDS | 50.0% |
| ACCAGCCAACAAGTTGTGAG+AGG | + | chr4_3:5145348-5145367 | None:intergenic | 50.0% | |
| !! | AACTTGTTGGCTGGTACGAC+TGG | - | chr4_3:5145353-5145372 | Msa0625790:CDS | 50.0% |
| TGGTACGACTGGAAAAGACG+AGG | - | chr4_3:5145364-5145383 | Msa0625790:CDS | 50.0% | |
| ! | CTCCAAGCATTGTCACTGTG+GGG | + | chr4_3:5144656-5144675 | None:intergenic | 50.0% |
| ! | CACTCCAAGCATTGTCACTG+TGG | + | chr4_3:5144658-5144677 | None:intergenic | 50.0% |
| CACAGTGACAATGCTTGGAG+TGG | - | chr4_3:5144656-5144675 | Msa0625790:CDS | 50.0% | |
| TGGAGTGGTTAAGGCACGTT+TGG | - | chr4_3:5144671-5144690 | Msa0625790:CDS | 50.0% | |
| GTGGTTAAGGCACGTTTGGT+TGG | - | chr4_3:5144675-5144694 | Msa0625790:CDS | 50.0% | |
| CGTTTGGTTGGAGCAACAAG+AGG | - | chr4_3:5144687-5144706 | Msa0625790:CDS | 50.0% | |
| TCTCGTACTGAGAATGTGGC+AGG | - | chr4_3:5144912-5144931 | Msa0625790:CDS | 50.0% | |
| !! | TGATTACAGTGCTGACGGTG+AGG | - | chr4_3:5144953-5144972 | Msa0625790:CDS | 50.0% |
| GCCACGAAGAATGATCTGAC+TGG | - | chr4_3:5144975-5144994 | Msa0625790:CDS | 50.0% | |
| !! | GATCTGACTGGATTGGCTAG+AGG | - | chr4_3:5144987-5145006 | Msa0625790:CDS | 50.0% |
| GTTGGATGAGCTTGAAAGGG+AGG | - | chr4_3:5145199-5145218 | Msa0625790:CDS | 50.0% | |
| !! | ACCTCTCACAACTTGTTGGC+TGG | - | chr4_3:5145344-5145363 | Msa0625790:CDS | 50.0% |
| ACCAGCCAACAAGTTGTGAG+AGG | + | chr4_3:5145348-5145367 | None:intergenic | 50.0% | |
| !! | AACTTGTTGGCTGGTACGAC+TGG | - | chr4_3:5145353-5145372 | Msa0625790:CDS | 50.0% |
| TGGTACGACTGGAAAAGACG+AGG | - | chr4_3:5145364-5145383 | Msa0625790:CDS | 50.0% | |
| GTCCCCACAGTGACAATGCT+TGG | - | chr4_3:5144651-5144670 | Msa0625790:CDS | 55.0% | |
| !! | CTGACTGGATTGGCTAGAGG+TGG | - | chr4_3:5144990-5145009 | Msa0625790:CDS | 55.0% |
| ATTGGCTAGAGGTGGTCAGC+AGG | - | chr4_3:5144998-5145017 | Msa0625790:CDS | 55.0% | |
| GTCCCCACAGTGACAATGCT+TGG | - | chr4_3:5144651-5144670 | Msa0625790:CDS | 55.0% | |
| !! | CTGACTGGATTGGCTAGAGG+TGG | - | chr4_3:5144990-5145009 | Msa0625790:CDS | 55.0% |
| ATTGGCTAGAGGTGGTCAGC+AGG | - | chr4_3:5144998-5145017 | Msa0625790:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 5144621 | 5145400 | 5144621 | ID=Msa0625790;Name=Msa0625790 |
| chr4_3 | mRNA | 5144621 | 5145400 | 5144621 | ID=Msa0625790-mRNA-1;Parent=Msa0625790;Name=Msa0625790-mRNA-1;_AED=0.22;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|259 |
| chr4_3 | exon | 5144621 | 5145400 | 5144621 | ID=Msa0625790-mRNA-1:exon:1428;Parent=Msa0625790-mRNA-1 |
| chr4_3 | CDS | 5144621 | 5145400 | 5144621 | ID=Msa0625790-mRNA-1:cds;Parent=Msa0625790-mRNA-1 |
| Gene Sequence |
| Protein sequence |
