AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0582620


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582620 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0582620 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0582620 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0582620 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa0582620 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 4_2:+1113106 None:intergenic
ACTGGCATAAAATCTACAAT+TGG 0.453871 4_2:-1113209 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 4_2:+1113756 Msa0582620:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 4_2:+1113744 Msa0582620:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 4_2:-1113762 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 4_2:-1113749 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG + chr4_2:1113396-1113415 Msa0582620:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG - chr4_2:1113402-1113421 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG - chr4_2:1113553-1113572 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG - chr4_2:1113394-1113413 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG + chr4_2:1113648-1113667 Msa0582620:intron 15.0%
!! GAAAAAACTACTTGAATAAA+GGG - chr4_2:1113153-1113172 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG - chr4_2:1113154-1113173 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG + chr4_2:1113154-1113173 Msa0582620:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG + chr4_2:1113440-1113459 Msa0582620:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG + chr4_2:1113491-1113510 Msa0582620:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG + chr4_2:1113582-1113601 Msa0582620:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG + chr4_2:1113246-1113265 Msa0582620:CDS 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG + chr4_2:1113723-1113742 Msa0582620:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG - chr4_2:1113195-1113214 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG - chr4_2:1113212-1113231 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG - chr4_2:1113230-1113249 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG - chr4_2:1113239-1113258 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG - chr4_2:1113240-1113259 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG - chr4_2:1113472-1113491 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG - chr4_2:1113475-1113494 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG + chr4_2:1113473-1113492 Msa0582620:intron 35.0%
CATATCCACAATCAAGATCT+AGG - chr4_2:1113481-1113500 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG + chr4_2:1113625-1113644 Msa0582620:intron 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG - chr4_2:1113188-1113207 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG + chr4_2:1113744-1113763 Msa0582620:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG - chr4_2:1113752-1113771 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG - chr4_2:1113469-1113488 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG + chr4_2:1113756-1113775 Msa0582620:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG - chr4_2:1113317-1113336 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG - chr4_2:1113765-1113784 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 1113107 1113791 1113107 ID=Msa0582620;Name=Msa0582620
chr4_2 mRNA 1113107 1113791 1113107 ID=Msa0582620-mRNA-1;Parent=Msa0582620;Name=Msa0582620-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr4_2 exon 1113107 1113281 1113107 ID=Msa0582620-mRNA-1:exon:261;Parent=Msa0582620-mRNA-1
chr4_2 exon 1113673 1113791 1113673 ID=Msa0582620-mRNA-1:exon:262;Parent=Msa0582620-mRNA-1
chr4_2 CDS 1113107 1113281 1113107 ID=Msa0582620-mRNA-1:cds;Parent=Msa0582620-mRNA-1
chr4_2 CDS 1113673 1113791 1113673 ID=Msa0582620-mRNA-1:cds;Parent=Msa0582620-mRNA-1
Gene Sequence

>Msa0582620

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa0582620

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*