AlfalfaGEDB | Msa0931150

M. sativa cultivar zhongmu-4 / Msa0931150

KEGG: K02710|K02712
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF02533.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0931150	RDY14692.1	90.722	97	9	0	1	97	4	100	7.75e-56	178

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0931150	sp\|Q49L15\|PSBK_EUCGG	91.667	60	5	0	1	60	1	60	1.80e-32	110

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0035090	Msa0931150	0.824309	8.656900e-54	-8.615850e-47
Msa0040190	Msa0931150	0.811692	6.164323e-51	-8.615850e-47
Msa0083570	Msa0931150	0.802877	4.555098e-49	-8.615850e-47
Msa0092750	Msa0931150	0.827139	1.845212e-54	-8.615850e-47
Msa0095140	Msa0931150	0.863981	1.687946e-64	-8.615850e-47
Msa0095200	Msa0931150	0.885876	5.571182e-72	-8.615850e-47
Msa0095240	Msa0931150	0.836022	1.193583e-56	-8.615850e-47
Msa0095270	Msa0931150	0.857487	1.578981e-62	-8.615850e-47
Msa0619060	Msa0931150	0.802334	5.893368e-49	-8.615850e-47
Msa0624240	Msa0931150	0.828044	1.118584e-54	-8.615850e-47
Msa0647110	Msa0931150	0.805293	1.431596e-49	-8.615850e-47
Msa0702070	Msa0931150	0.805677	1.189365e-49	-8.615850e-47
Msa0702120	Msa0931150	0.802639	5.099464e-49	-8.615850e-47
Msa0704750	Msa0931150	0.804820	1.798080e-49	-8.615850e-47
Msa0705610	Msa0931150	0.810922	9.057916e-51	-8.615850e-47
Msa0706710	Msa0931150	0.800009	1.761978e-48	-8.615850e-47
Msa0708120	Msa0931150	0.800857	1.184040e-48	-8.615850e-47
Msa0727020	Msa0931150	0.858759	6.607730e-63	-8.615850e-47
Msa0766880	Msa0931150	0.835319	1.798245e-56	-8.615850e-47
Msa0772210	Msa0931150	0.813347	2.680687e-51	-8.615850e-47
Msa0804930	Msa0931150	0.816990	4.158913e-52	-8.615850e-47
Msa0821490	Msa0931150	0.805877	1.079517e-49	-8.615850e-47
Msa0130120	Msa0931150	0.815952	7.101213e-52	-8.615850e-47
Msa0133880	Msa0931150	0.801622	8.255774e-49	-8.615850e-47
Msa0134550	Msa0931150	0.814165	1.769919e-51	-8.615850e-47
Msa0139740	Msa0931150	0.810426	1.159610e-50	-8.615850e-47
Msa0175900	Msa0931150	0.821348	4.235054e-53	-8.615850e-47
Msa0190000	Msa0931150	0.804596	2.002109e-49	-8.615850e-47
Msa0216010	Msa0931150	0.800270	1.559112e-48	-8.615850e-47
Msa0221860	Msa0931150	0.831685	1.451052e-55	-8.615850e-47
Msa0222070	Msa0931150	0.814196	1.742054e-51	-8.615850e-47
Msa0222120	Msa0931150	0.813442	2.553975e-51	-8.615850e-47
Msa0227290	Msa0931150	0.815474	9.074595e-52	-8.615850e-47
Msa0377460	Msa0931150	0.805259	1.455439e-49	-8.615850e-47
Msa0426860	Msa0931150	0.810645	1.039890e-50	-8.615850e-47
Msa0426890	Msa0931150	0.805254	1.458821e-49	-8.615850e-47
Msa0427050	Msa0931150	0.814545	1.458701e-51	-8.615850e-47
Msa0473450	Msa0931150	0.805014	1.637433e-49	-8.615850e-47
Msa0482020	Msa0931150	0.809834	1.555590e-50	-8.615850e-47
Msa0253410	Msa0931150	0.806743	7.091903e-50	-8.615850e-47
Msa0263620	Msa0931150	0.812285	4.578999e-51	-8.615850e-47
Msa0268180	Msa0931150	0.902223	1.207963e-78	-8.615850e-47
Msa0268220	Msa0931150	0.829344	5.424287e-55	-8.615850e-47
Msa0268340	Msa0931150	0.838795	2.325850e-57	-8.615850e-47
Msa0268380	Msa0931150	0.871475	6.641837e-67	-8.615850e-47
Msa0268420	Msa0931150	0.938015	1.350137e-98	-8.615850e-47
Msa0268460	Msa0931150	0.826432	2.721338e-54	-8.615850e-47
Msa0268500	Msa0931150	0.840855	6.763726e-58	-8.615850e-47
Msa0268540	Msa0931150	0.918880	9.019123e-87	-8.615850e-47
Msa0268650	Msa0931150	0.931071	6.487663e-94	-8.615850e-47
Msa0268680	Msa0931150	0.805721	1.164148e-49	-8.615850e-47
Msa0268690	Msa0931150	0.801863	7.366649e-49	-8.615850e-47
Msa0268730	Msa0931150	0.857819	1.258821e-62	-8.615850e-47
Msa0268760	Msa0931150	0.834340	3.171483e-56	-8.615850e-47
Msa0268850	Msa0931150	0.909702	4.250946e-82	-8.615850e-47
Msa0268900	Msa0931150	0.837192	6.009914e-57	-8.615850e-47
Msa0268930	Msa0931150	0.858812	6.371114e-63	-8.615850e-47
Msa0269060	Msa0931150	0.910497	1.753818e-82	-8.615850e-47
Msa0269100	Msa0931150	0.834506	2.881024e-56	-8.615850e-47
Msa0301800	Msa0931150	0.809823	1.564352e-50	-8.615850e-47
Msa0306130	Msa0931150	0.805827	1.105991e-49	-8.615850e-47
Msa0307490	Msa0931150	0.816338	5.822906e-52	-8.615850e-47
Msa0324060	Msa0931150	0.802869	4.571596e-49	-8.615850e-47
Msa0344200	Msa0931150	0.852126	5.665319e-61	-8.615850e-47
Msa0344250	Msa0931150	0.825609	4.269821e-54	-8.615850e-47
Msa0344290	Msa0931150	0.803627	3.184857e-49	-8.615850e-47
Msa0517400	Msa0931150	0.800963	1.126216e-48	-8.615850e-47
Msa0577140	Msa0931150	0.810284	1.244528e-50	-8.615850e-47
Msa0582530	Msa0931150	0.883057	6.190778e-71	-8.615850e-47
Msa0582570	Msa0931150	0.831870	1.306427e-55	-8.615850e-47
Msa0582580	Msa0931150	0.836310	1.008343e-56	-8.615850e-47
Msa0582620	Msa0931150	0.871789	5.226878e-67	-8.615850e-47
Msa0582660	Msa0931150	0.800320	1.522884e-48	-8.615850e-47
Msa0582710	Msa0931150	0.926134	7.111870e-91	-8.615850e-47
Msa0582740	Msa0931150	0.876752	1.087317e-68	-8.615850e-47
Msa0582830	Msa0931150	0.924316	8.294198e-90	-8.615850e-47
Msa0582900	Msa0931150	0.862247	5.800346e-64	-8.615850e-47
Msa0582930	Msa0931150	0.813178	2.918726e-51	-8.615850e-47
Msa0591370	Msa0931150	0.817432	3.307847e-52	-8.615850e-47
Msa0595780	Msa0931150	0.810790	9.674193e-51	-8.615850e-47
Msa0855490	Msa0931150	0.826156	3.166731e-54	-8.615850e-47
Msa0860270	Msa0931150	0.801291	9.649358e-49	-8.615850e-47
Msa0910650	Msa0931150	0.803540	3.320043e-49	-8.615850e-47
Msa0924820	Msa0931150	0.800762	1.237680e-48	-8.615850e-47
Msa0924860	Msa0931150	0.820234	7.637804e-53	-8.615850e-47
Msa0924870	Msa0931150	0.804070	2.576379e-49	-8.615850e-47
Msa0924890	Msa0931150	0.835115	2.024383e-56	-8.615850e-47
Msa0925020	Msa0931150	0.822588	2.186271e-53	-8.615850e-47
Msa0931090	Msa0931150	0.930754	1.032619e-93	-8.615850e-47
Msa0931150	Msa0931200	0.813473	2.514008e-51	-8.615850e-47
Msa0931150	Msa0931280	0.910300	2.185344e-82	-8.615850e-47
Msa0931150	Msa0983670	0.826602	2.479338e-54	-8.615850e-47
Msa0931150	Msa0983710	0.821104	4.821747e-53	-8.615850e-47
Msa0931150	Msa0992570	0.814627	1.399029e-51	-8.615850e-47
Msa0931150	Msa0992590	0.811448	6.965783e-51	-8.615850e-47
Msa0931150	Msa1002040	0.802180	6.339035e-49	-8.615850e-47
Msa0931150	Msa1030280	0.826823	2.195914e-54	-8.615850e-47
Msa0931150	Msa1049440	0.804966	1.675625e-49	-8.615850e-47
Msa0931150	Msa1086020	0.851172	1.054863e-60	-8.615850e-47
Msa0931150	Msa1086060	0.877158	7.863334e-69	-8.615850e-47
Msa0931150	Msa1086160	0.870847	1.070878e-66	-8.615850e-47
Msa0931150	Msa1086170	0.885471	7.903345e-72	-8.615850e-47
Msa0931150	Msa1086260	0.834751	2.500753e-56	-8.615850e-47
Msa0931150	Msa1086290	0.926836	2.709371e-91	-8.615850e-47
Msa0931150	Msa1086330	0.824615	7.331303e-54	-8.615850e-47
Msa0931150	Msa1086340	0.831985	1.223767e-55	-8.615850e-47
Msa0931150	Msa1086370	0.887386	1.493280e-72	-8.615850e-47
Msa0931150	Msa1086440	0.852750	3.760530e-61	-8.615850e-47
Msa0931150	Msa1086480	0.891634	3.321708e-74	-8.615850e-47
Msa0931150	Msa1086520	0.830482	2.864194e-55	-8.615850e-47
Msa0931150	Msa1086630	0.842991	1.843724e-58	-8.615850e-47
Msa0931150	Msa1086690	0.830574	2.720154e-55	-8.615850e-47
Msa0931150	Msa1086830	0.846936	1.588174e-59	-8.615850e-47
Msa0931150	Msa1086870	0.858883	6.065520e-63	-8.615850e-47
Msa0931150	Msa1090230	0.818863	1.569622e-52	-8.615850e-47
Msa0931150	Msa1091690	0.855431	6.336976e-62	-8.615850e-47
Msa0931150	Msa1124440	0.801609	8.305322e-49	-8.615850e-47
Msa0931150	Msa1130700	0.805129	1.549116e-49	-8.615850e-47
Msa0931150	Msa1143140	0.880373	5.789545e-70	-8.615850e-47
Msa0931150	Msa1143150	0.832573	8.749636e-56	-8.615850e-47
Msa0931150	Msa1157160	0.831573	1.546642e-55	-8.615850e-47
Msa0931150	Msa1173410	0.802453	5.569243e-49	-8.615850e-47
Msa0931150	Msa1195860	0.807136	5.858078e-50	-8.615850e-47
Msa0931150	Msa1198420	0.815728	7.967141e-52	-8.615850e-47
Msa0931150	Msa1209390	0.814061	1.865983e-51	-8.615850e-47
Msa0931150	Msa1298740	0.806097	9.703748e-50	-8.615850e-47
Msa0931150	Msa1302920	0.810709	1.007211e-50	-8.615850e-47
Msa0931150	Msa1309910	0.813961	1.963794e-51	-8.615850e-47
Msa0931150	Msa1327820	0.806233	9.085246e-50	-8.615850e-47
Msa0931150	Msa1354850	0.862707	4.189099e-64	-8.615850e-47
Msa0931150	Msa1354890	0.877862	4.469609e-69	-8.615850e-47
Msa0931150	Msa1354930	0.806169	9.374312e-50	-8.615850e-47
Msa0931150	Msa1355020	0.847399	1.185491e-59	-8.615850e-47
Msa0931150	Msa1355060	0.881373	2.533343e-70	-8.615850e-47
Msa0931150	Msa1355100	0.804960	1.681152e-49	-8.615850e-47
Msa0931150	Msa1367810	0.803942	2.739789e-49	-8.615850e-47
Msa0931150	Msa1371250	0.805163	1.524122e-49	-8.615850e-47
Msa0931150	Msa1380540	0.841218	5.429641e-58	-8.615850e-47
Msa0931150	Msa1380580	0.803574	3.267547e-49	-8.615850e-47
Msa0931150	Msa1385940	0.823928	1.063514e-53	-8.615850e-47
Msa0931150	Msa1394090	0.846637	1.916905e-59	-8.615850e-47
Msa0931150	Msa1394100	0.829349	5.410133e-55	-8.615850e-47
Msa0931150	Msa1394140	0.825065	5.743285e-54	-8.615850e-47
Msa0931150	Msa1394170	0.810749	9.874837e-51	-8.615850e-47
Msa0931150	Msa1394240	0.874996	4.361080e-68	-8.615850e-47
Msa0931150	Msa1394280	0.840832	6.855665e-58	-8.615850e-47
Msa0931150	Msa1406230	0.809769	1.606440e-50	-8.615850e-47
Msa0931150	Msa1415120	0.826741	2.296882e-54	-8.615850e-47
Msa0931150	Msa1426870	0.808681	2.748951e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0931150	MtrunA17_Chr4g0015681	96.667	60	2	1	60	1	60	7.64e-35	113
Msa0931150	MtrunA17_CPg0492901	96.667	60	2	1	60	1	60	7.64e-35	113
Msa0931150	MtrunA17_Chr4g0016771	96.667	60	2	1	60	1	60	7.64e-35	113

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0931150	ATCG00070.1	76.667	60	14	0	1	60	1	60	3.76e-26	92.0
Msa0931150	ATCG00080.1	97.222	36	1	0	62	97	1	36	2.92e-19	73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGCTTAATATCTTTAGCT+TGG	0.440922	6_2:-80361219	Msa0931150:CDS
ACTGGCATAAAATCTACAAT+TGG	0.453871	6_2:+80361116	None:intergenic
AATGATCCAGGACGTAATCC+TGG	0.474800	6_2:-80360569	Msa0931150:CDS
GGATTCCTATCTAATGATCC+AGG	0.536202	6_2:-80360581	Msa0931150:CDS
TCACGTCCAGGATTACGTCC+TGG	0.548081	6_2:+80360563	None:intergenic
TACGTCCTGGATCATTAGAT+AGG	0.581605	6_2:+80360576	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTCCAAATATTAAAATTTT+TGG	-	chr6_2:80360845-80360864	Msa0931150:intron	10.0%
!!!	TATCCAAAAATTTTAATATT+TGG	+	chr6_2:80360851-80360870	None:intergenic	10.0%
!!	ATTTCAAAAAAAAAAAACTT+AGG	+	chr6_2:80361002-80361021	None:intergenic	10.0%
!!!	AATTTTAATATTTGGAATCA+AGG	+	chr6_2:80360843-80360862	None:intergenic	15.0%
!!!	CGTTTTTTTTTTAATTATCT+TGG	-	chr6_2:80361097-80361116	Msa0931150:CDS	15.0%
!!	GAAAAAACTACTTGAATAAA+GGG	+	chr6_2:80360602-80360621	None:intergenic	20.0%
!!	AGAAAAAACTACTTGAATAA+AGG	+	chr6_2:80360603-80360622	None:intergenic	20.0%
!!!	TTATTCAAGTAGTTTTTTCT+TGG	-	chr6_2:80360603-80360622	Msa0931150:CDS	20.0%
!!	TGAAAATTCTATTATTCGAT+TGG	-	chr6_2:80360889-80360908	Msa0931150:intron	20.0%
!!!	TGTGGATATGAAAAAAATTT+TGG	-	chr6_2:80360940-80360959	Msa0931150:intron	20.0%
!!	TCTTATAAACTTAGTATCAA+AGG	-	chr6_2:80361031-80361050	Msa0931150:intron	20.0%
!!!	TTTTTCTCTTAGCTTTTGTT+TGG	-	chr6_2:80360695-80360714	Msa0931150:intron	25.0%
!!!	TTTGTTTCTCTTTTCATCTT+CGG	-	chr6_2:80361172-80361191	Msa0931150:CDS	25.0%
!	AATTGGATTCAAAAAAGCGT+AGG	+	chr6_2:80360644-80360663	None:intergenic	30.0%
	ACTGGCATAAAATCTACAAT+TGG	+	chr6_2:80360661-80360680	None:intergenic	30.0%
!!	AAAAAGAGTAAGGGTATTAC+TGG	+	chr6_2:80360679-80360698	None:intergenic	30.0%
	GCTAAGAGAAAAAAGAGTAA+GGG	+	chr6_2:80360688-80360707	None:intergenic	30.0%
	AGCTAAGAGAAAAAAGAGTA+AGG	+	chr6_2:80360689-80360708	None:intergenic	30.0%
!!	AATCAAGATCTAGGTATTGG+TGG	+	chr6_2:80360921-80360940	None:intergenic	35.0%
	CACAATCAAGATCTAGGTAT+TGG	+	chr6_2:80360924-80360943	None:intergenic	35.0%
	CAATACCTAGATCTTGATTG+TGG	-	chr6_2:80360922-80360941	Msa0931150:intron	35.0%
	CATATCCACAATCAAGATCT+AGG	+	chr6_2:80360930-80360949	None:intergenic	35.0%
	GAGAATCTATTCTCTTTCTC+TGG	-	chr6_2:80361074-80361093	Msa0931150:CDS	35.0%
!	TTCAAAAAAGCGTAGGCTTC+AGG	+	chr6_2:80360637-80360656	None:intergenic	40.0%
	GGATTCCTATCTAATGATCC+AGG	-	chr6_2:80361193-80361212	Msa0931150:CDS	40.0%
	TACGTCCTGGATCATTAGAT+AGG	+	chr6_2:80361201-80361220	None:intergenic	40.0%
!!	CAAGATCTAGGTATTGGTGG+TGG	+	chr6_2:80360918-80360937	None:intergenic	45.0%
	AATGATCCAGGACGTAATCC+TGG	-	chr6_2:80361205-80361224	Msa0931150:CDS	45.0%
!!!	AATAAATTCTTAATTTTTTT+CGG	+	chr6_2:80360766-80360785	None:intergenic	5.0%
	TCACGTCCAGGATTACGTCC+TGG	+	chr6_2:80361214-80361233	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	80360556	80361240	80360556	ID=Msa0931150;Name=Msa0931150
chr6_2	mRNA	80360556	80361240	80360556	ID=Msa0931150-mRNA-1;Parent=Msa0931150;Name=Msa0931150-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0\|0\|0\|1\|0\|0\|2\|0\|97
chr6_2	exon	80361066	80361240	80361066	ID=Msa0931150-mRNA-1:exon:14078;Parent=Msa0931150-mRNA-1
chr6_2	exon	80360556	80360674	80360556	ID=Msa0931150-mRNA-1:exon:14077;Parent=Msa0931150-mRNA-1
chr6_2	CDS	80361066	80361240	80361066	ID=Msa0931150-mRNA-1:cds;Parent=Msa0931150-mRNA-1
chr6_2	CDS	80360556	80360674	80360556	ID=Msa0931150-mRNA-1:cds;Parent=Msa0931150-mRNA-1

Gene Sequence	>Msa0931150 ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA
Protein sequence	>Msa0931150 MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*