AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0931090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0931090 MtrunA17_Chr4g0015681 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0931090 MtrunA17_CPg0492901 96.667 60 2 0 1 60 1 60 7.64e-35 113
Msa0931090 MtrunA17_Chr4g0016771 96.667 60 2 0 1 60 1 60 7.64e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0931090 ATCG00070.1 76.667 60 14 0 1 60 1 60 3.76e-26 92.0
Msa0931090 ATCG00080.1 97.222 36 1 0 62 97 1 36 2.92e-19 73.9

Find 6 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 6_2:-80324686 Msa0931090:CDS
ACTGGCATAAAATCTACAAT+TGG 0.453871 6_2:+80324583 None:intergenic
AATGATCCAGGACGTAATCC+TGG 0.474800 6_2:-80324036 Msa0931090:CDS
GGATTCCTATCTAATGATCC+AGG 0.536202 6_2:-80324048 Msa0931090:CDS
TCACGTCCAGGATTACGTCC+TGG 0.548081 6_2:+80324030 None:intergenic
TACGTCCTGGATCATTAGAT+AGG 0.581605 6_2:+80324043 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCCAAATATTAAAATTTT+TGG - chr6_2:80324312-80324331 Msa0931090:intron 10.0%
!!! TATCCAAAAATTTTAATATT+TGG + chr6_2:80324318-80324337 None:intergenic 10.0%
!! ATTTCAAAAAAAAAAAACTT+AGG + chr6_2:80324469-80324488 None:intergenic 10.0%
!!! AATTTTAATATTTGGAATCA+AGG + chr6_2:80324310-80324329 None:intergenic 15.0%
!!! CGTTTTTTTTTTAATTATCT+TGG - chr6_2:80324564-80324583 Msa0931090:CDS 15.0%
!! GAAAAAACTACTTGAATAAA+GGG + chr6_2:80324069-80324088 None:intergenic 20.0%
!! AGAAAAAACTACTTGAATAA+AGG + chr6_2:80324070-80324089 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG - chr6_2:80324070-80324089 Msa0931090:CDS 20.0%
!! TGAAAATTCTATTATTCGAT+TGG - chr6_2:80324356-80324375 Msa0931090:intron 20.0%
!!! TGTGGATATGAAAAAAATTT+TGG - chr6_2:80324407-80324426 Msa0931090:intron 20.0%
!! TCTTATAAACTTAGTATCAA+AGG - chr6_2:80324498-80324517 Msa0931090:intron 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG - chr6_2:80324162-80324181 Msa0931090:intron 25.0%
!!! TTTGTTTCTCTTTTCATCTT+CGG - chr6_2:80324639-80324658 Msa0931090:CDS 25.0%
! AATTGGATTCAAAAAAGCGT+AGG + chr6_2:80324111-80324130 None:intergenic 30.0%
ACTGGCATAAAATCTACAAT+TGG + chr6_2:80324128-80324147 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG + chr6_2:80324146-80324165 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG + chr6_2:80324155-80324174 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG + chr6_2:80324156-80324175 None:intergenic 30.0%
!! AATCAAGATCTAGGTATTGG+TGG + chr6_2:80324388-80324407 None:intergenic 35.0%
CACAATCAAGATCTAGGTAT+TGG + chr6_2:80324391-80324410 None:intergenic 35.0%
CAATACCTAGATCTTGATTG+TGG - chr6_2:80324389-80324408 Msa0931090:intron 35.0%
CATATCCACAATCAAGATCT+AGG + chr6_2:80324397-80324416 None:intergenic 35.0%
GAGAATCTATTCTCTTTCTC+TGG - chr6_2:80324541-80324560 Msa0931090:CDS 35.0%
! TTCAAAAAAGCGTAGGCTTC+AGG + chr6_2:80324104-80324123 None:intergenic 40.0%
GGATTCCTATCTAATGATCC+AGG - chr6_2:80324660-80324679 Msa0931090:CDS 40.0%
TACGTCCTGGATCATTAGAT+AGG + chr6_2:80324668-80324687 None:intergenic 40.0%
!! CAAGATCTAGGTATTGGTGG+TGG + chr6_2:80324385-80324404 None:intergenic 45.0%
AATGATCCAGGACGTAATCC+TGG - chr6_2:80324672-80324691 Msa0931090:CDS 45.0%
!!! AATAAATTCTTAATTTTTTT+CGG + chr6_2:80324233-80324252 None:intergenic 5.0%
TCACGTCCAGGATTACGTCC+TGG + chr6_2:80324681-80324700 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 80324023 80324707 80324023 ID=Msa0931090;Name=Msa0931090
chr6_2 mRNA 80324023 80324707 80324023 ID=Msa0931090-mRNA-1;Parent=Msa0931090;Name=Msa0931090-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|97
chr6_2 exon 80324533 80324707 80324533 ID=Msa0931090-mRNA-1:exon:14076;Parent=Msa0931090-mRNA-1
chr6_2 exon 80324023 80324141 80324023 ID=Msa0931090-mRNA-1:exon:14075;Parent=Msa0931090-mRNA-1
chr6_2 CDS 80324533 80324707 80324533 ID=Msa0931090-mRNA-1:cds;Parent=Msa0931090-mRNA-1
chr6_2 CDS 80324023 80324141 80324023 ID=Msa0931090-mRNA-1:cds;Parent=Msa0931090-mRNA-1
Gene Sequence

>Msa0931090

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAATTTTGTAATGCTTACTCTAAAACTATTTGTTTACACGATAGTGATATTCTTTGTTTCTCTTTTCATCTTCGGATTCCTATCTAATGATCCAGGACGTAATCCTGGACGTGAAGAATAA

Protein sequence

>Msa0931090

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVNFVMLTLKLFVYTIVIFFVSLFIFGFLSNDPGRNPGREE*