Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1150850 | XP_003623204.1 | 98.190 | 221 | 4 | 0 | 1 | 221 | 1 | 221 | 1.06e-156 | 442 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1150850 | sp|P32110|GSTX6_SOYBN | 67.265 | 223 | 68 | 2 | 1 | 218 | 1 | 223 | 3.49e-100 | 293 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1150850 | G7L6H8 | 98.190 | 221 | 4 | 0 | 1 | 221 | 1 | 221 | 5.08e-157 | 442 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0030160 | Msa1150850 | 0.801775 | 7.680505e-49 | -8.615850e-47 |
| Msa0079110 | Msa1150850 | 0.804751 | 1.858802e-49 | -8.615850e-47 |
| Msa0092410 | Msa1150850 | 0.801880 | 7.308808e-49 | -8.615850e-47 |
| Msa0688390 | Msa1150850 | 0.801434 | 9.019932e-49 | -8.615850e-47 |
| Msa0698290 | Msa1150850 | 0.803350 | 3.634647e-49 | -8.615850e-47 |
| Msa0703850 | Msa1150850 | 0.805079 | 1.587218e-49 | -8.615850e-47 |
| Msa0711150 | Msa1150850 | 0.814976 | 1.170883e-51 | -8.615850e-47 |
| Msa0755050 | Msa1150850 | 0.807560 | 4.763072e-50 | -8.615850e-47 |
| Msa0795070 | Msa1150850 | 0.820949 | 5.235286e-53 | -8.615850e-47 |
| Msa0828240 | Msa1150850 | 0.815054 | 1.124952e-51 | -8.615850e-47 |
| Msa0187030 | Msa1150850 | 0.825145 | 5.498293e-54 | -8.615850e-47 |
| Msa0207350 | Msa1150850 | 0.830083 | 3.585798e-55 | -8.615850e-47 |
| Msa0215590 | Msa1150850 | 0.805529 | 1.277275e-49 | -8.615850e-47 |
| Msa0217700 | Msa1150850 | 0.809617 | 1.731482e-50 | -8.615850e-47 |
| Msa0227340 | Msa1150850 | 0.810174 | 1.313914e-50 | -8.615850e-47 |
| Msa0369920 | Msa1150850 | 0.803842 | 2.874383e-49 | -8.615850e-47 |
| Msa0397460 | Msa1150850 | 0.805735 | 1.156359e-49 | -8.615850e-47 |
| Msa0417330 | Msa1150850 | 0.816240 | 6.124086e-52 | -8.615850e-47 |
| Msa0467100 | Msa1150850 | 0.823568 | 1.291754e-53 | -8.615850e-47 |
| Msa0468410 | Msa1150850 | 0.813778 | 2.154314e-51 | -8.615850e-47 |
| Msa1028590 | Msa1150850 | 0.800027 | 1.746939e-48 | -8.615850e-47 |
| Msa0241140 | Msa1150850 | 0.817912 | 2.577580e-52 | -8.615850e-47 |
| Msa0255850 | Msa1150850 | 0.822154 | 2.757668e-53 | -8.615850e-47 |
| Msa0264830 | Msa1150850 | 0.818984 | 1.473149e-52 | -8.615850e-47 |
| Msa0269560 | Msa1150850 | 0.809106 | 2.229128e-50 | -8.615850e-47 |
| Msa0278570 | Msa1150850 | 0.804433 | 2.165000e-49 | -8.615850e-47 |
| Msa0296480 | Msa1150850 | 0.807781 | 4.274889e-50 | -8.615850e-47 |
| Msa0296820 | Msa1150850 | 0.800142 | 1.655948e-48 | -8.615850e-47 |
| Msa0302870 | Msa1150850 | 0.815544 | 8.755372e-52 | -8.615850e-47 |
| Msa0333650 | Msa1150850 | 0.803987 | 2.681056e-49 | -8.615850e-47 |
| Msa0340340 | Msa1150850 | 0.814053 | 1.874028e-51 | -8.615850e-47 |
| Msa1142670 | Msa1150850 | 0.816597 | 5.094090e-52 | -8.615850e-47 |
| Msa1150850 | Msa1188710 | 0.807225 | 5.608118e-50 | -8.615850e-47 |
| Msa1150850 | Msa1238170 | 0.810340 | 1.210406e-50 | -8.615850e-47 |
| Msa1150850 | Msa1243870 | 0.803652 | 3.147320e-49 | -8.615850e-47 |
| Msa1150850 | Msa1330180 | 0.805484 | 1.305366e-49 | -8.615850e-47 |
| Msa1150850 | Msa1355890 | 0.800279 | 1.552959e-48 | -8.615850e-47 |
| Msa1150850 | Msa1418240 | 0.822561 | 2.217725e-53 | -8.615850e-47 |
| Msa1150850 | Msa1418280 | 0.804152 | 2.477298e-49 | -8.615850e-47 |
| Msa1150850 | Msa1433350 | 0.812984 | 3.219005e-51 | -8.615850e-47 |
| Msa0485240 | Msa1150850 | 0.821529 | 3.846130e-53 | -8.615850e-47 |
| Msa0506860 | Msa1150850 | 0.800610 | 1.329509e-48 | -8.615850e-47 |
| Msa0549890 | Msa1150850 | 0.817819 | 2.706066e-52 | -8.615850e-47 |
| Msa0558360 | Msa1150850 | 0.800954 | 1.130855e-48 | -8.615850e-47 |
| Msa0880810 | Msa1150850 | 0.801108 | 1.051751e-48 | -8.615850e-47 |
| Msa0902830 | Msa1150850 | 0.819357 | 1.211600e-52 | -8.615850e-47 |
| Msa0936380 | Msa1150850 | 0.807978 | 3.881575e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1150850 | MtrunA17_Chr7g0240651 | 98.190 | 221 | 4 | 0 | 1 | 221 | 1 | 221 | 9.79e-161 | 442 |
| Msa1150850 | MtrunA17_Chr7g0240611 | 85.068 | 221 | 33 | 0 | 1 | 221 | 1 | 221 | 1.71e-137 | 384 |
| Msa1150850 | MtrunA17_Chr7g0240671 | 82.381 | 210 | 34 | 1 | 1 | 210 | 1 | 207 | 2.63e-127 | 358 |
| Msa1150850 | MtrunA17_Chr7g0240661 | 87.143 | 210 | 27 | 0 | 1 | 210 | 1 | 210 | 1.09e-125 | 354 |
| Msa1150850 | MtrunA17_Chr7g0240561 | 65.766 | 222 | 72 | 1 | 1 | 218 | 1 | 222 | 1.19e-103 | 298 |
| Msa1150850 | MtrunA17_Chr7g0240641 | 65.333 | 225 | 74 | 1 | 1 | 221 | 1 | 225 | 4.52e-100 | 290 |
| Msa1150850 | MtrunA17_Chr7g0240581 | 65.315 | 222 | 73 | 1 | 1 | 218 | 1 | 222 | 6.11e-99 | 286 |
| Msa1150850 | MtrunA17_Chr7g0240511 | 67.890 | 218 | 69 | 1 | 1 | 218 | 1 | 217 | 7.80e-99 | 286 |
| Msa1150850 | MtrunA17_Chr7g0240631 | 64.889 | 225 | 75 | 1 | 1 | 221 | 1 | 225 | 5.22e-98 | 284 |
| Msa1150850 | MtrunA17_Chr7g0240681 | 65.158 | 221 | 72 | 2 | 1 | 216 | 1 | 221 | 7.87e-97 | 281 |
| Msa1150850 | MtrunA17_Chr7g0240551 | 62.557 | 219 | 78 | 1 | 1 | 215 | 1 | 219 | 1.05e-93 | 273 |
| Msa1150850 | MtrunA17_Chr7g0240531 | 63.426 | 216 | 75 | 1 | 1 | 212 | 1 | 216 | 2.15e-92 | 270 |
| Msa1150850 | MtrunA17_Chr7g0240691 | 61.538 | 221 | 80 | 2 | 6 | 221 | 36 | 256 | 3.29e-92 | 271 |
| Msa1150850 | MtrunA17_Chr7g0240541 | 60.648 | 216 | 81 | 1 | 1 | 212 | 1 | 216 | 6.88e-89 | 261 |
| Msa1150850 | MtrunA17_Chr4g0008791 | 56.889 | 225 | 93 | 1 | 1 | 221 | 1 | 225 | 2.24e-85 | 252 |
| Msa1150850 | MtrunA17_Chr7g0240521 | 61.435 | 223 | 81 | 2 | 1 | 218 | 8 | 230 | 5.12e-83 | 246 |
| Msa1150850 | MtrunA17_Chr4g0008781 | 57.339 | 218 | 87 | 3 | 2 | 214 | 3 | 219 | 1.92e-82 | 244 |
| Msa1150850 | MtrunA17_Chr6g0483141 | 46.154 | 208 | 106 | 3 | 7 | 209 | 3 | 209 | 1.36e-59 | 187 |
| Msa1150850 | MtrunA17_Chr3g0132581 | 47.059 | 221 | 111 | 3 | 5 | 220 | 3 | 222 | 6.91e-58 | 182 |
| Msa1150850 | MtrunA17_Chr8g0379561 | 40.541 | 222 | 126 | 3 | 5 | 221 | 4 | 224 | 2.43e-56 | 178 |
| Msa1150850 | MtrunA17_Chr8g0379581 | 43.662 | 213 | 114 | 3 | 5 | 212 | 3 | 214 | 5.08e-53 | 170 |
| Msa1150850 | MtrunA17_Chr5g0417011 | 40.991 | 222 | 119 | 4 | 1 | 211 | 1 | 221 | 6.20e-52 | 167 |
| Msa1150850 | MtrunA17_Chr1g0196771 | 43.458 | 214 | 112 | 4 | 6 | 212 | 2 | 213 | 8.46e-52 | 167 |
| Msa1150850 | MtrunA17_Chr1g0196691 | 41.892 | 222 | 120 | 4 | 7 | 220 | 28 | 248 | 1.35e-51 | 167 |
| Msa1150850 | MtrunA17_Chr1g0196661 | 41.981 | 212 | 111 | 4 | 7 | 209 | 3 | 211 | 4.63e-50 | 162 |
| Msa1150850 | MtrunA17_Chr1g0212301 | 44.608 | 204 | 102 | 4 | 7 | 201 | 3 | 204 | 2.05e-49 | 160 |
| Msa1150850 | MtrunA17_Chr4g0029341 | 39.252 | 214 | 120 | 4 | 6 | 211 | 3 | 214 | 4.96e-47 | 154 |
| Msa1150850 | MtrunA17_Chr1g0196721 | 41.981 | 212 | 113 | 4 | 8 | 211 | 3 | 212 | 5.41e-47 | 154 |
| Msa1150850 | MtrunA17_Chr5g0415411 | 40.090 | 222 | 121 | 3 | 7 | 216 | 5 | 226 | 3.94e-46 | 152 |
| Msa1150850 | MtrunA17_Chr2g0312771 | 39.713 | 209 | 117 | 5 | 6 | 208 | 3 | 208 | 8.79e-45 | 149 |
| Msa1150850 | MtrunA17_Chr2g0312731 | 42.439 | 205 | 109 | 6 | 6 | 204 | 3 | 204 | 1.89e-44 | 148 |
| Msa1150850 | MtrunA17_Chr2g0312751 | 39.524 | 210 | 118 | 5 | 6 | 209 | 3 | 209 | 2.00e-44 | 148 |
| Msa1150850 | MtrunA17_Chr1g0196711 | 39.524 | 210 | 117 | 4 | 7 | 208 | 3 | 210 | 3.24e-44 | 147 |
| Msa1150850 | MtrunA17_Chr3g0107731 | 40.609 | 197 | 112 | 5 | 16 | 208 | 18 | 213 | 5.68e-41 | 139 |
| Msa1150850 | MtrunA17_Chr4g0069471 | 47.904 | 167 | 81 | 5 | 3 | 166 | 21 | 184 | 1.14e-40 | 137 |
| Msa1150850 | MtrunA17_Chr2g0312811 | 37.264 | 212 | 122 | 6 | 6 | 209 | 3 | 211 | 1.29e-40 | 138 |
| Msa1150850 | MtrunA17_Chr8g0357241 | 47.239 | 163 | 81 | 4 | 6 | 166 | 3 | 162 | 4.87e-40 | 136 |
| Msa1150850 | MtrunA17_Chr8g0357211 | 47.239 | 163 | 81 | 4 | 6 | 166 | 3 | 162 | 5.25e-40 | 136 |
| Msa1150850 | MtrunA17_Chr2g0312791 | 44.172 | 163 | 87 | 3 | 6 | 166 | 3 | 163 | 6.95e-40 | 136 |
| Msa1150850 | MtrunA17_Chr8g0363751 | 38.249 | 217 | 122 | 4 | 7 | 211 | 5 | 221 | 9.29e-40 | 136 |
| Msa1150850 | MtrunA17_Chr2g0312781 | 36.667 | 210 | 124 | 5 | 1 | 204 | 15 | 221 | 3.62e-39 | 135 |
| Msa1150850 | MtrunA17_Chr3g0107741 | 37.799 | 209 | 121 | 5 | 6 | 208 | 8 | 213 | 1.90e-38 | 132 |
| Msa1150850 | MtrunA17_Chr2g0312721 | 36.453 | 203 | 124 | 4 | 6 | 204 | 3 | 204 | 6.38e-38 | 131 |
| Msa1150850 | MtrunA17_Chr2g0312691 | 35.945 | 217 | 130 | 5 | 7 | 217 | 4 | 217 | 9.91e-38 | 130 |
| Msa1150850 | MtrunA17_Chr2g0312681 | 35.577 | 208 | 125 | 5 | 7 | 208 | 4 | 208 | 1.09e-36 | 128 |
| Msa1150850 | MtrunA17_Chr5g0433801 | 38.164 | 207 | 123 | 4 | 6 | 208 | 5 | 210 | 1.24e-35 | 125 |
| Msa1150850 | MtrunA17_Chr2g0312711 | 36.406 | 217 | 128 | 6 | 7 | 217 | 4 | 216 | 6.84e-34 | 120 |
| Msa1150850 | MtrunA17_Chr8g0391631 | 43.590 | 156 | 78 | 4 | 7 | 153 | 21 | 175 | 1.43e-31 | 113 |
| Msa1150850 | MtrunA17_Chr2g0313561 | 39.130 | 69 | 40 | 2 | 51 | 118 | 2 | 69 | 4.84e-13 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1150850 | AT3G09270.1 | 51.584 | 221 | 99 | 5 | 5 | 218 | 4 | 223 | 1.89e-69 | 212 |
| Msa1150850 | AT2G29420.1 | 45.455 | 220 | 114 | 3 | 6 | 220 | 8 | 226 | 4.59e-65 | 201 |
| Msa1150850 | AT2G29450.1 | 44.749 | 219 | 112 | 4 | 6 | 215 | 5 | 223 | 7.10e-54 | 172 |
| Msa1150850 | AT2G29480.1 | 46.119 | 219 | 105 | 5 | 1 | 210 | 1 | 215 | 1.07e-53 | 172 |
| Msa1150850 | AT2G29490.1 | 43.750 | 224 | 119 | 4 | 1 | 218 | 1 | 223 | 1.44e-53 | 172 |
| Msa1150850 | AT2G29460.1 | 45.370 | 216 | 111 | 4 | 1 | 210 | 1 | 215 | 1.86e-53 | 171 |
| Msa1150850 | AT2G29470.1 | 41.333 | 225 | 124 | 5 | 1 | 218 | 1 | 224 | 6.96e-52 | 167 |
| Msa1150850 | AT2G29440.1 | 40.909 | 220 | 123 | 4 | 5 | 218 | 4 | 222 | 2.35e-48 | 158 |
| Msa1150850 | AT1G10370.1 | 41.232 | 211 | 111 | 4 | 1 | 200 | 1 | 209 | 5.57e-46 | 152 |
| Msa1150850 | AT1G59700.1 | 43.913 | 230 | 114 | 7 | 6 | 220 | 5 | 234 | 1.09e-45 | 152 |
| Msa1150850 | AT1G59670.1 | 42.512 | 207 | 107 | 5 | 6 | 200 | 5 | 211 | 4.37e-45 | 150 |
| Msa1150850 | AT1G27140.1 | 38.667 | 225 | 121 | 5 | 3 | 212 | 2 | 224 | 3.38e-44 | 148 |
| Msa1150850 | AT1G69930.1 | 38.532 | 218 | 122 | 5 | 5 | 210 | 10 | 227 | 7.68e-44 | 147 |
| Msa1150850 | AT1G78340.1 | 48.214 | 168 | 84 | 3 | 6 | 171 | 3 | 169 | 3.22e-43 | 145 |
| Msa1150850 | AT1G10360.1 | 38.350 | 206 | 116 | 4 | 6 | 200 | 4 | 209 | 1.09e-42 | 144 |
| Msa1150850 | AT1G27130.1 | 37.838 | 222 | 126 | 5 | 3 | 212 | 2 | 223 | 2.79e-42 | 143 |
| Msa1150850 | AT1G78320.1 | 39.130 | 207 | 117 | 5 | 4 | 204 | 1 | 204 | 5.63e-42 | 142 |
| Msa1150850 | AT1G78370.1 | 40.394 | 203 | 116 | 5 | 10 | 208 | 7 | 208 | 5.71e-42 | 142 |
| Msa1150850 | AT1G17170.1 | 38.889 | 216 | 126 | 5 | 6 | 217 | 3 | 216 | 5.91e-41 | 139 |
| Msa1150850 | AT1G78380.1 | 40.196 | 204 | 113 | 5 | 7 | 204 | 4 | 204 | 1.75e-40 | 138 |
| Msa1150850 | AT1G17180.1 | 39.901 | 203 | 117 | 5 | 6 | 204 | 3 | 204 | 5.84e-40 | 137 |
| Msa1150850 | AT1G53680.1 | 36.493 | 211 | 129 | 5 | 1 | 206 | 1 | 211 | 2.12e-38 | 133 |
| Msa1150850 | AT5G62480.1 | 38.627 | 233 | 123 | 8 | 4 | 217 | 1 | 232 | 5.58e-38 | 132 |
| Msa1150850 | AT1G69920.1 | 37.799 | 209 | 118 | 5 | 5 | 201 | 32 | 240 | 2.94e-37 | 130 |
| Msa1150850 | AT1G78360.1 | 39.070 | 215 | 118 | 7 | 1 | 208 | 1 | 209 | 3.32e-37 | 129 |
| Msa1150850 | AT1G17190.1 | 38.172 | 186 | 110 | 5 | 5 | 186 | 3 | 187 | 1.10e-35 | 125 |
| Msa1150850 | AT3G43800.1 | 38.049 | 205 | 118 | 7 | 5 | 201 | 3 | 206 | 5.86e-35 | 124 |
| Msa1150850 | AT1G74590.1 | 35.961 | 203 | 117 | 5 | 7 | 198 | 7 | 207 | 5.03e-31 | 114 |
| Msa1150850 | AT1G78320.2 | 32.039 | 206 | 106 | 5 | 4 | 204 | 1 | 177 | 8.80e-26 | 99.4 |
| Msa1150850 | AT5G62480.2 | 31.466 | 232 | 115 | 8 | 4 | 217 | 1 | 206 | 5.44e-21 | 87.4 |
| Msa1150850 | AT5G62480.3 | 31.466 | 232 | 114 | 8 | 4 | 217 | 1 | 205 | 5.85e-21 | 87.4 |
Find 52 sgRNAs with CRISPR-Local
Find 137 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCAATTTCTCAATTAATT+TGG | 0.166798 | 7_4:-34689898 | None:intergenic |
| TCAAATACAATCCAGTTTAT+AGG | 0.216244 | 7_4:+34690070 | Msa1150850:CDS |
| ACTGTTTCTCTGCTACTTCT+TGG | 0.238294 | 7_4:-34691829 | None:intergenic |
| TATCTTTATGTTTAACTCTA+AGG | 0.238671 | 7_4:-34689852 | None:intergenic |
| ACTTGACTTTGTTGAGTTCT+TGG | 0.247717 | 7_4:+34692051 | Msa1150850:three_prime_UTR |
| TCTGCTACTTCTTGGAATAA+AGG | 0.258191 | 7_4:-34691821 | None:intergenic |
| GTTCCTGTTCTTGTTCATAA+TGG | 0.264740 | 7_4:+34690096 | Msa1150850:CDS |
| GATGAAGTTCAAATCCCTTA+TGG | 0.266625 | 7_4:+34692248 | Msa1150850:three_prime_UTR |
| GAGCTAAAGGACAAGTTCTT+TGG | 0.282556 | 7_4:+34691755 | Msa1150850:CDS |
| AAAGAGCCTTGGATCGGTTT+TGG | 0.296896 | 7_4:+34690205 | Msa1150850:CDS |
| TGATGAAGAAGTTCAGCTTT+TGG | 0.304952 | 7_4:+34689939 | Msa1150850:CDS |
| CCTTCAAAGGAGCAACAATT+TGG | 0.305433 | 7_4:+34691932 | Msa1150850:CDS |
| CTGCTACTTCTTGGAATAAA+GGG | 0.326236 | 7_4:-34691820 | None:intergenic |
| TTCCTGTTCTTGTTCATAAT+GGG | 0.343118 | 7_4:+34690097 | Msa1150850:CDS |
| CCAAATTGTTGCTCCTTTGA+AGG | 0.353336 | 7_4:-34691932 | None:intergenic |
| TAAGGATCATTAGGCAAGAT+TGG | 0.355374 | 7_4:-34690180 | None:intergenic |
| ATCACACGAGACTCAGAAAT+AGG | 0.380094 | 7_4:-34690123 | None:intergenic |
| ACAGGAACCATCCTATAAAC+TGG | 0.382808 | 7_4:-34690081 | None:intergenic |
| GGCATAACTTCTTTGACAAC+TGG | 0.383415 | 7_4:-34691911 | None:intergenic |
| GTCAAAATTCATAGATGAAA+AGG | 0.387976 | 7_4:+34690227 | Msa1150850:CDS |
| TTCTCGCGCTCTTTCTCATC+AGG | 0.391480 | 7_4:-34691689 | None:intergenic |
| GGGAAGTCCATTTGTGATAA+GGG | 0.397256 | 7_4:+34689969 | Msa1150850:CDS |
| ATTTGAACCCTTATCACAAA+TGG | 0.404722 | 7_4:-34689976 | None:intergenic |
| TGGGAAGTCCATTTGTGATA+AGG | 0.415245 | 7_4:+34689968 | Msa1150850:CDS |
| TTCTTTGGAGGACAAGAGAT+TGG | 0.418946 | 7_4:+34691770 | Msa1150850:CDS |
| ATGAAGTTCAAATCCCTTAT+GGG | 0.423623 | 7_4:+34692249 | Msa1150850:three_prime_UTR |
| TGTTTGAATATCAGTTCAAA+TGG | 0.426966 | 7_4:+34692075 | Msa1150850:three_prime_UTR |
| GATTTGAACTTCATCCTTTG+AGG | 0.431012 | 7_4:-34692240 | None:intergenic |
| TTGAGTATATTGATGAGGCT+TGG | 0.451367 | 7_4:+34690148 | Msa1150850:CDS |
| AGCTTGGGAAATTTGTCAGC+AGG | 0.453112 | 7_4:-34691857 | None:intergenic |
| TTCCCATTATGAACAAGAAC+AGG | 0.453502 | 7_4:-34690099 | None:intergenic |
| AGGACAAGAGATTGGCTTTG+TGG | 0.456715 | 7_4:+34691778 | Msa1150850:CDS |
| CAAAGAAGTTATGCCTTCAA+AGG | 0.497169 | 7_4:+34691919 | Msa1150850:CDS |
| CAAATTGCTCTGAAATTGAA+AGG | 0.499164 | 7_4:+34689994 | Msa1150850:CDS |
| GTTTCTTGAGAATGAGCTAA+AGG | 0.504881 | 7_4:+34691742 | Msa1150850:CDS |
| TTGCTATTTCAATTAATGAA+AGG | 0.515675 | 7_4:+34692146 | Msa1150850:three_prime_UTR |
| CTAAAGGACAAGTTCTTTGG+AGG | 0.516999 | 7_4:+34691758 | Msa1150850:CDS |
| TGATCCTTACAAAAGAGCCT+TGG | 0.527046 | 7_4:+34690194 | Msa1150850:CDS |
| GGCTCGTGAAGAATTATTCG+AGG | 0.551878 | 7_4:+34691712 | Msa1150850:CDS |
| AGGAATTGAACACAAATATG+TGG | 0.552045 | 7_4:+34690014 | Msa1150850:CDS |
| GTTTAACTTTGTAACCTCAA+AGG | 0.554826 | 7_4:+34692226 | Msa1150850:three_prime_UTR |
| TAACTTCTTTGACAACTGGA+TGG | 0.565089 | 7_4:-34691907 | None:intergenic |
| ATACAATCCAGTTTATAGGA+TGG | 0.576358 | 7_4:+34690074 | Msa1150850:CDS |
| CTTACAAAAGAGCCTTGGAT+CGG | 0.586658 | 7_4:+34690199 | Msa1150850:CDS |
| GCTTGGGAAATTTGTCAGCA+GGG | 0.597981 | 7_4:-34691856 | None:intergenic |
| TGATGAGAAAGAGCGCGAGA+AGG | 0.601244 | 7_4:+34691691 | Msa1150850:CDS |
| GAGAAATTGAAGAACTACAA+TGG | 0.605007 | 7_4:+34689909 | Msa1150850:exon |
| ATCAGTGTACGATTGCTGCA+TGG | 0.617894 | 7_4:+34691651 | Msa1150850:intron |
| ATGTTTGTAATTCATATGTG+AGG | 0.627487 | 7_4:+34692184 | Msa1150850:three_prime_UTR |
| TATGTGAGGTGTGAGCTCAT+TGG | 0.627786 | 7_4:+34692198 | Msa1150850:three_prime_UTR |
| GATTCTTGAGTATATTGATG+AGG | 0.639329 | 7_4:+34690143 | Msa1150850:CDS |
| TCACATATGAATTACAAACA+TGG | 0.655628 | 7_4:-34692182 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATAAAATTCTATTTTGA+TGG | - | chr7_4:34691992-34692011 | None:intergenic | 10.0% |
| !!! | AATTTCACCAAATTTTTTAA+TGG | - | chr7_4:34690433-34690452 | None:intergenic | 15.0% |
| !! | ATTTCATTCATGAATTATAT+CGG | + | chr7_4:34690450-34690469 | Msa1150850:intron | 15.0% |
| !! | GTTCTATTTATTTATGTTAT+GGG | - | chr7_4:34690685-34690704 | None:intergenic | 15.0% |
| !! | AGTTCTATTTATTTATGTTA+TGG | - | chr7_4:34690686-34690705 | None:intergenic | 15.0% |
| !! | TATCTTTATGTTTAACTCTA+AGG | - | chr7_4:34689855-34689874 | None:intergenic | 20.0% |
| !!! | TTTTATTAAGCAATTTGTGT+TGG | - | chr7_4:34689878-34689897 | None:intergenic | 20.0% |
| !! | CTTCAATTTCTCAATTAATT+TGG | - | chr7_4:34689901-34689920 | None:intergenic | 20.0% |
| !! | TTATTTATGTTATGGGTTTA+GGG | - | chr7_4:34690678-34690697 | None:intergenic | 20.0% |
| !! | TTTATTTATGTTATGGGTTT+AGG | - | chr7_4:34690679-34690698 | None:intergenic | 20.0% |
| !!! | CAATTTTCTTTTAACTATAC+TGG | + | chr7_4:34690784-34690803 | Msa1150850:intron | 20.0% |
| !! | CATAACTAAATTTGATCAAT+TGG | - | chr7_4:34690850-34690869 | None:intergenic | 20.0% |
| !! | AAAAATGAAATACAAAACAC+AGG | - | chr7_4:34691154-34691173 | None:intergenic | 20.0% |
| !!! | TTGCTATTTCAATTAATGAA+AGG | + | chr7_4:34692146-34692165 | Msa1150850:three_prime_UTR | 20.0% |
| ! | AAATATGTGGAAGAAAAACT+GGG | + | chr7_4:34690027-34690046 | Msa1150850:CDS | 25.0% |
| ! | TCAAATACAATCCAGTTTAT+AGG | + | chr7_4:34690070-34690089 | Msa1150850:CDS | 25.0% |
| ! | GTCAAAATTCATAGATGAAA+AGG | + | chr7_4:34690227-34690246 | Msa1150850:CDS | 25.0% |
| ! | GATAGTATACATCTCATAAA+AGG | - | chr7_4:34690283-34690302 | None:intergenic | 25.0% |
| !!! | AATTTTTTAATGGTTGACAG+TGG | - | chr7_4:34690423-34690442 | None:intergenic | 25.0% |
| ! | CTGTCAACCATTAAAAAATT+TGG | + | chr7_4:34690423-34690442 | Msa1150850:intron | 25.0% |
| ! | ATCATAACAAACGTTTAAAG+TGG | - | chr7_4:34690875-34690894 | None:intergenic | 25.0% |
| !! | GTTATGATGTTCAGATTTTA+AGG | + | chr7_4:34690887-34690906 | Msa1150850:intron | 25.0% |
| !!! | TCTACTTTAGAATGAATGTT+TGG | + | chr7_4:34691079-34691098 | Msa1150850:intron | 25.0% |
| !!! | GTATTTCGAGTTATAGAAAT+GGG | - | chr7_4:34691233-34691252 | None:intergenic | 25.0% |
| !!! | TGTATTTCGAGTTATAGAAA+TGG | - | chr7_4:34691234-34691253 | None:intergenic | 25.0% |
| ! | ATCAGTAAGAAACAAATATG+TGG | - | chr7_4:34691312-34691331 | None:intergenic | 25.0% |
| !! | TTTGTTTCTTACTGATGTAA+TGG | + | chr7_4:34691316-34691335 | Msa1150850:intron | 25.0% |
| ! | CAAATTCATAGATAATCACA+TGG | - | chr7_4:34691493-34691512 | None:intergenic | 25.0% |
| ! | TGTTTGAATATCAGTTCAAA+TGG | + | chr7_4:34692075-34692094 | Msa1150850:three_prime_UTR | 25.0% |
| ! | AAAAATGACAACAATCTCTT+AGG | - | chr7_4:34692128-34692147 | None:intergenic | 25.0% |
| ! | TCACATATGAATTACAAACA+TGG | - | chr7_4:34692185-34692204 | None:intergenic | 25.0% |
| ! | ATGTTTGTAATTCATATGTG+AGG | + | chr7_4:34692184-34692203 | Msa1150850:three_prime_UTR | 25.0% |
| GAGAAATTGAAGAACTACAA+TGG | + | chr7_4:34689909-34689928 | Msa1150850:exon | 30.0% | |
| ATTTGAACCCTTATCACAAA+TGG | - | chr7_4:34689979-34689998 | None:intergenic | 30.0% | |
| !! | CAAATTGCTCTGAAATTGAA+AGG | + | chr7_4:34689994-34690013 | Msa1150850:CDS | 30.0% |
| AGGAATTGAACACAAATATG+TGG | + | chr7_4:34690014-34690033 | Msa1150850:CDS | 30.0% | |
| CAAATATGTGGAAGAAAAAC+TGG | + | chr7_4:34690026-34690045 | Msa1150850:CDS | 30.0% | |
| ATACAATCCAGTTTATAGGA+TGG | + | chr7_4:34690074-34690093 | Msa1150850:CDS | 30.0% | |
| TTCCTGTTCTTGTTCATAAT+GGG | + | chr7_4:34690097-34690116 | Msa1150850:CDS | 30.0% | |
| GATTCTTGAGTATATTGATG+AGG | + | chr7_4:34690143-34690162 | Msa1150850:CDS | 30.0% | |
| !! | CAGTGGACTATATGTTAAAT+TGG | - | chr7_4:34690406-34690425 | None:intergenic | 30.0% |
| CTCATATATAGACACAAAAG+AGG | + | chr7_4:34690495-34690514 | Msa1150850:intron | 30.0% | |
| TAACTGCTCTATAATCGTAA+AGG | + | chr7_4:34690707-34690726 | Msa1150850:intron | 30.0% | |
| AACCAAACATACAGTAGTAT+AGG | + | chr7_4:34691117-34691136 | Msa1150850:intron | 30.0% | |
| !! | TTGGTTTTTAAAAGATGCAG+TGG | + | chr7_4:34691194-34691213 | Msa1150850:intron | 30.0% |
| TTCTTACTGATGTAATGGTA+AGG | + | chr7_4:34691321-34691340 | Msa1150850:intron | 30.0% | |
| !! | TAATTCGTGTCGTTTGTTAT+GGG | - | chr7_4:34691376-34691395 | None:intergenic | 30.0% |
| AAAGTTCACATTTAGTGACA+TGG | - | chr7_4:34691589-34691608 | None:intergenic | 30.0% | |
| AATTTCCCAAGCTACAAAAA+TGG | + | chr7_4:34691867-34691886 | Msa1150850:CDS | 30.0% | |
| ! | GTTTAACTTTGTAACCTCAA+AGG | + | chr7_4:34692226-34692245 | Msa1150850:three_prime_UTR | 30.0% |
| ATGAAGTTCAAATCCCTTAT+GGG | + | chr7_4:34692249-34692268 | Msa1150850:three_prime_UTR | 30.0% | |
| ! | ATTTTACAACTGTCCCATAA+GGG | - | chr7_4:34692265-34692284 | None:intergenic | 30.0% |
| ! | TGATGAAGAAGTTCAGCTTT+TGG | + | chr7_4:34689939-34689958 | Msa1150850:CDS | 35.0% |
| ! | GATGAAGAAGTTCAGCTTTT+GGG | + | chr7_4:34689940-34689959 | Msa1150850:CDS | 35.0% |
| GTTCCTGTTCTTGTTCATAA+TGG | + | chr7_4:34690096-34690115 | Msa1150850:CDS | 35.0% | |
| TTCCCATTATGAACAAGAAC+AGG | - | chr7_4:34690102-34690121 | None:intergenic | 35.0% | |
| TTGAGTATATTGATGAGGCT+TGG | + | chr7_4:34690148-34690167 | Msa1150850:CDS | 35.0% | |
| TAAGGATCATTAGGCAAGAT+TGG | - | chr7_4:34690183-34690202 | None:intergenic | 35.0% | |
| ! | GCTCTTTTGTAAGGATCATT+AGG | - | chr7_4:34690192-34690211 | None:intergenic | 35.0% |
| !! | TCAAGGGTATTGCTGTAATT+TGG | + | chr7_4:34690651-34690670 | Msa1150850:intron | 35.0% |
| CTCCTATACTACTGTATGTT+TGG | - | chr7_4:34691122-34691141 | None:intergenic | 35.0% | |
| AGAAAACAAGAGAAGATCCT+TGG | + | chr7_4:34691261-34691280 | Msa1150850:intron | 35.0% | |
| GATGTAATGGTAAGGTTACA+AGG | + | chr7_4:34691329-34691348 | Msa1150850:intron | 35.0% | |
| !! | CTAATTCGTGTCGTTTGTTA+TGG | - | chr7_4:34691377-34691396 | None:intergenic | 35.0% |
| ATGGTAGATGAATTCTAGCA+TGG | - | chr7_4:34691570-34691589 | None:intergenic | 35.0% | |
| GTTTCTTGAGAATGAGCTAA+AGG | + | chr7_4:34691742-34691761 | Msa1150850:CDS | 35.0% | |
| ! | CTGCTACTTCTTGGAATAAA+GGG | - | chr7_4:34691823-34691842 | None:intergenic | 35.0% |
| ! | TCTGCTACTTCTTGGAATAA+AGG | - | chr7_4:34691824-34691843 | None:intergenic | 35.0% |
| !! | AGACTCCATTTTTGTAGCTT+GGG | - | chr7_4:34691875-34691894 | None:intergenic | 35.0% |
| !! | TAGACTCCATTTTTGTAGCT+TGG | - | chr7_4:34691876-34691895 | None:intergenic | 35.0% |
| TAACTTCTTTGACAACTGGA+TGG | - | chr7_4:34691910-34691929 | None:intergenic | 35.0% | |
| CAAAGAAGTTATGCCTTCAA+AGG | + | chr7_4:34691919-34691938 | Msa1150850:CDS | 35.0% | |
| ACTTGACTTTGTTGAGTTCT+TGG | + | chr7_4:34692051-34692070 | Msa1150850:three_prime_UTR | 35.0% | |
| GATTTGAACTTCATCCTTTG+AGG | - | chr7_4:34692243-34692262 | None:intergenic | 35.0% | |
| GATGAAGTTCAAATCCCTTA+TGG | + | chr7_4:34692248-34692267 | Msa1150850:three_prime_UTR | 35.0% | |
| ! | CATTTTACAACTGTCCCATA+AGG | - | chr7_4:34692266-34692285 | None:intergenic | 35.0% |
| TGGGAAGTCCATTTGTGATA+AGG | + | chr7_4:34689968-34689987 | Msa1150850:CDS | 40.0% | |
| GGGAAGTCCATTTGTGATAA+GGG | + | chr7_4:34689969-34689988 | Msa1150850:CDS | 40.0% | |
| ACAGGAACCATCCTATAAAC+TGG | - | chr7_4:34690084-34690103 | None:intergenic | 40.0% | |
| ATCACACGAGACTCAGAAAT+AGG | - | chr7_4:34690126-34690145 | None:intergenic | 40.0% | |
| TGATCCTTACAAAAGAGCCT+TGG | + | chr7_4:34690194-34690213 | Msa1150850:CDS | 40.0% | |
| CTTACAAAAGAGCCTTGGAT+CGG | + | chr7_4:34690199-34690218 | Msa1150850:CDS | 40.0% | |
| ! | TTTTGACCAAAACCGATCCA+AGG | - | chr7_4:34690214-34690233 | None:intergenic | 40.0% |
| AATTGAGCCTAACACACGTT+CGG | - | chr7_4:34690592-34690611 | None:intergenic | 40.0% | |
| GTGTTAGGCTCAATTCATGT+TGG | + | chr7_4:34690597-34690616 | Msa1150850:intron | 40.0% | |
| TTAGGCTCAATTCATGTTGG+AGG | + | chr7_4:34690600-34690619 | Msa1150850:intron | 40.0% | |
| TAGGCTCAATTCATGTTGGA+GGG | + | chr7_4:34690601-34690620 | Msa1150850:intron | 40.0% | |
| ATCGTAAAGGTAAGCACCAT+TGG | + | chr7_4:34690720-34690739 | Msa1150850:intron | 40.0% | |
| CTTGACACACTTACACAACT+TGG | + | chr7_4:34691175-34691194 | Msa1150850:intron | 40.0% | |
| AGCATGGATCACACATTATG+TGG | - | chr7_4:34691554-34691573 | None:intergenic | 40.0% | |
| GAGCTAAAGGACAAGTTCTT+TGG | + | chr7_4:34691755-34691774 | Msa1150850:CDS | 40.0% | |
| CTAAAGGACAAGTTCTTTGG+AGG | + | chr7_4:34691758-34691777 | Msa1150850:CDS | 40.0% | |
| ! | TTCTTTGGAGGACAAGAGAT+TGG | + | chr7_4:34691770-34691789 | Msa1150850:CDS | 40.0% |
| ! | ACTGTTTCTCTGCTACTTCT+TGG | - | chr7_4:34691832-34691851 | None:intergenic | 40.0% |
| GGCATAACTTCTTTGACAAC+TGG | - | chr7_4:34691914-34691933 | None:intergenic | 40.0% | |
| CCAAATTGTTGCTCCTTTGA+AGG | - | chr7_4:34691935-34691954 | None:intergenic | 40.0% | |
| CCTTCAAAGGAGCAACAATT+TGG | + | chr7_4:34691932-34691951 | Msa1150850:CDS | 40.0% | |
| !!! | TCTATTTTGATGGAGCAGCA+AGG | - | chr7_4:34691982-34692001 | None:intergenic | 40.0% |
| !!! | AGTTCAGCTTTTGGGTTCAG+TGG | + | chr7_4:34689948-34689967 | Msa1150850:CDS | 45.0% |
| !!! | GTTCAGCTTTTGGGTTCAGT+GGG | + | chr7_4:34689949-34689968 | Msa1150850:CDS | 45.0% |
| ! | CGATCCAAGGCTCTTTTGTA+AGG | - | chr7_4:34690201-34690220 | None:intergenic | 45.0% |
| !! | AAAGAGCCTTGGATCGGTTT+TGG | + | chr7_4:34690205-34690224 | Msa1150850:CDS | 45.0% |
| ! | ATAGACACAAAAGAGGCGCA+AGG | + | chr7_4:34690502-34690521 | Msa1150850:intron | 45.0% |
| ! | TAGACACAAAAGAGGCGCAA+GGG | + | chr7_4:34690503-34690522 | Msa1150850:intron | 45.0% |
| ATCAGTGTACGATTGCTGCA+TGG | + | chr7_4:34691651-34691670 | Msa1150850:intron | 45.0% | |
| GGCTCGTGAAGAATTATTCG+AGG | + | chr7_4:34691712-34691731 | Msa1150850:CDS | 45.0% | |
| !! | AGGACAAGAGATTGGCTTTG+TGG | + | chr7_4:34691778-34691797 | Msa1150850:CDS | 45.0% |
| GCTTGGGAAATTTGTCAGCA+GGG | - | chr7_4:34691859-34691878 | None:intergenic | 45.0% | |
| AGCTTGGGAAATTTGTCAGC+AGG | - | chr7_4:34691860-34691879 | None:intergenic | 45.0% | |
| TATGTGAGGTGTGAGCTCAT+TGG | + | chr7_4:34692198-34692217 | Msa1150850:three_prime_UTR | 45.0% | |
| GCACAATCACAGCAGTCACA+TGG | - | chr7_4:34690305-34690324 | None:intergenic | 50.0% | |
| ! | AGACACAAAAGAGGCGCAAG+GGG | + | chr7_4:34690504-34690523 | Msa1150850:intron | 50.0% |
| TCATGTTGGAGGGAGACTAG+TGG | + | chr7_4:34690611-34690630 | Msa1150850:intron | 50.0% | |
| CATGTTGGAGGGAGACTAGT+GGG | + | chr7_4:34690612-34690631 | Msa1150850:intron | 50.0% | |
| TGTTCACGCAGTTCAGCCAA+TGG | - | chr7_4:34690739-34690758 | None:intergenic | 50.0% | |
| CAAGAGAAGATCCTTGGCAG+TGG | + | chr7_4:34691267-34691286 | Msa1150850:intron | 50.0% | |
| AAACACTGTCTCCACTGCCA+AGG | - | chr7_4:34691281-34691300 | None:intergenic | 50.0% | |
| TTCTCGCGCTCTTTCTCATC+AGG | - | chr7_4:34691692-34691711 | None:intergenic | 50.0% | |
| ! | TGATGAGAAAGAGCGCGAGA+AGG | + | chr7_4:34691691-34691710 | Msa1150850:CDS | 50.0% |
| GTTTGGGCCGAACGTGTGTT+AGG | + | chr7_4:34690582-34690601 | Msa1150850:intron | 55.0% | |
| !! | TTCAGGTGGCGTCGGTTCAA+GGG | + | chr7_4:34690635-34690654 | Msa1150850:intron | 55.0% |
| ! | CACAAAAGAGGCGCAAGGGG+TGG | + | chr7_4:34690507-34690526 | Msa1150850:intron | 60.0% |
| CAGGGGGAGGGAACTTTGCA+GGG | - | chr7_4:34690555-34690574 | None:intergenic | 60.0% | |
| ACAGGGGGAGGGAACTTTGC+AGG | - | chr7_4:34690556-34690575 | None:intergenic | 60.0% | |
| CAAACGCAAGGACAGGGGGA+GGG | - | chr7_4:34690567-34690586 | None:intergenic | 60.0% | |
| CTCCCCCTGTCCTTGCGTTT+GGG | + | chr7_4:34690566-34690585 | Msa1150850:intron | 60.0% | |
| TCGGCCCAAACGCAAGGACA+GGG | - | chr7_4:34690573-34690592 | None:intergenic | 60.0% | |
| TTCGGCCCAAACGCAAGGAC+AGG | - | chr7_4:34690574-34690593 | None:intergenic | 60.0% | |
| ACACGTTCGGCCCAAACGCA+AGG | - | chr7_4:34690579-34690598 | None:intergenic | 60.0% | |
| !! | GGAGGGAGACTAGTGGGTTC+AGG | + | chr7_4:34690618-34690637 | Msa1150850:intron | 60.0% |
| !! | GGGAGACTAGTGGGTTCAGG+TGG | + | chr7_4:34690621-34690640 | Msa1150850:intron | 60.0% |
| !! | CTAGTGGGTTCAGGTGGCGT+CGG | + | chr7_4:34690627-34690646 | Msa1150850:intron | 60.0% |
| !! | GTTCAGGTGGCGTCGGTTCA+AGG | + | chr7_4:34690634-34690653 | Msa1150850:intron | 60.0% |
| CCAAACGCAAGGACAGGGGG+AGG | - | chr7_4:34690568-34690587 | None:intergenic | 65.0% | |
| CCTCCCCCTGTCCTTGCGTT+TGG | + | chr7_4:34690565-34690584 | Msa1150850:intron | 65.0% | |
| GGCCCAAACGCAAGGACAGG+GGG | - | chr7_4:34690571-34690590 | None:intergenic | 65.0% | |
| CGGCCCAAACGCAAGGACAG+GGG | - | chr7_4:34690572-34690591 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 34689833 | 34692376 | 34689833 | ID=Msa1150850;Name=Msa1150850 |
| chr7_4 | mRNA | 34689833 | 34692376 | 34689833 | ID=Msa1150850-mRNA-1;Parent=Msa1150850;Name=Msa1150850-mRNA-1;_AED=0.04;_eAED=0.04;_QI=95|1|1|1|1|1|2|376|221 |
| chr7_4 | exon | 34689833 | 34690248 | 34689833 | ID=Msa1150850-mRNA-1:exon:8226;Parent=Msa1150850-mRNA-1 |
| chr7_4 | exon | 34691656 | 34692376 | 34691656 | ID=Msa1150850-mRNA-1:exon:8227;Parent=Msa1150850-mRNA-1 |
| chr7_4 | five_prime_UTR | 34689833 | 34689927 | 34689833 | ID=Msa1150850-mRNA-1:five_prime_utr;Parent=Msa1150850-mRNA-1 |
| chr7_4 | CDS | 34689928 | 34690248 | 34689928 | ID=Msa1150850-mRNA-1:cds;Parent=Msa1150850-mRNA-1 |
| chr7_4 | CDS | 34691656 | 34692000 | 34691656 | ID=Msa1150850-mRNA-1:cds;Parent=Msa1150850-mRNA-1 |
| chr7_4 | three_prime_UTR | 34692001 | 34692376 | 34692001 | ID=Msa1150850-mRNA-1:three_prime_utr;Parent=Msa1150850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
