AlfalfaGEDB | Msa1201240

M. sativa cultivar zhongmu-4 / Msa1201240

KEGG: -
GO: GO:0000724 GO:0000725 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006109 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009378 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010565 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0030656 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043255 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0045912 GO:0046137 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051193 GO:0051195 GO:0051196 GO:0051198 GO:0051276 GO:0051716 GO:0062012 GO:0062014 GO:0065007 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901363 GO:2000082 GO:2000083
PFAM: PF03478.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1201240	XP_003629214.1	91.270	378	32	1	106	483	1	377	0.0	704

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1201240	sp\|Q3EBZ2\|SKI23_ARATH	31.135	379	224	13	110	466	2	365	3.86e-38	147

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0012040	Msa1201240	0.816087	6.626211e-52	-8.615850e-47
Msa0020300	Msa1201240	0.833633	4.766469e-56	-8.615850e-47
Msa0052640	Msa1201240	0.807823	4.187233e-50	-8.615850e-47
Msa0052770	Msa1201240	0.817401	3.362008e-52	-8.615850e-47
Msa0113750	Msa1201240	0.826418	2.742448e-54	-8.615850e-47
Msa0610430	Msa1201240	0.825098	5.640354e-54	-8.615850e-47
Msa0663240	Msa1201240	0.802527	5.378763e-49	-8.615850e-47
Msa0669440	Msa1201240	0.800430	1.446954e-48	-8.615850e-47
Msa0692830	Msa1201240	0.813785	2.146739e-51	-8.615850e-47
Msa0692950	Msa1201240	0.820395	7.018070e-53	-8.615850e-47
Msa0715700	Msa1201240	0.800700	1.274170e-48	-8.615850e-47
Msa0716840	Msa1201240	0.813535	2.436903e-51	-8.615850e-47
Msa0737600	Msa1201240	0.807926	3.980740e-50	-8.615850e-47
Msa0743720	Msa1201240	0.813923	2.001613e-51	-8.615850e-47
Msa0769920	Msa1201240	0.815999	6.932936e-52	-8.615850e-47
Msa0771720	Msa1201240	0.804500	2.096773e-49	-8.615850e-47
Msa0824680	Msa1201240	0.808914	2.449973e-50	-8.615850e-47
Msa0150820	Msa1201240	0.822585	2.189566e-53	-8.615850e-47
Msa0159180	Msa1201240	0.844700	6.425711e-59	-8.615850e-47
Msa0159220	Msa1201240	0.819394	1.188243e-52	-8.615850e-47
Msa0433360	Msa1201240	0.800180	1.626765e-48	-8.615850e-47
Msa0433640	Msa1201240	0.808944	2.414961e-50	-8.615850e-47
Msa0442200	Msa1201240	0.803430	3.499636e-49	-8.615850e-47
Msa0477080	Msa1201240	0.805540	1.271027e-49	-8.615850e-47
Msa1051200	Msa1201240	0.817169	3.791669e-52	-8.615850e-47
Msa1082500	Msa1201240	0.812479	4.152436e-51	-8.615850e-47
Msa0255080	Msa1201240	0.802870	4.569835e-49	-8.615850e-47
Msa0298700	Msa1201240	0.810381	1.186043e-50	-8.615850e-47
Msa0309770	Msa1201240	0.806341	8.624244e-50	-8.615850e-47
Msa1131220	Msa1201240	0.801530	8.623270e-49	-8.615850e-47
Msa1173140	Msa1201240	0.816040	6.786925e-52	-8.615850e-47
Msa1174040	Msa1201240	0.806002	1.016382e-49	-8.615850e-47
Msa1197410	Msa1201240	0.800429	1.447445e-48	-8.615850e-47
Msa1199220	Msa1201240	0.802003	6.893768e-49	-8.615850e-47
Msa1201240	Msa1250890	0.812814	3.508256e-51	-8.615850e-47
Msa1201240	Msa1253020	0.807726	4.391113e-50	-8.615850e-47
Msa1201240	Msa1305340	0.807546	4.795509e-50	-8.615850e-47
Msa1201240	Msa1385770	0.810054	1.395044e-50	-8.615850e-47
Msa1201240	Msa1409240	0.809790	1.589949e-50	-8.615850e-47
Msa1201240	Msa1424450	0.800490	1.406416e-48	-8.615850e-47
Msa1201240	Msa1427230	0.805552	1.263467e-49	-8.615850e-47
Msa1201240	Msa1434140	0.822896	1.853697e-53	-8.615850e-47
Msa1201240	Msa1435370	0.810618	1.054187e-50	-8.615850e-47
Msa1201240	Msa1440910	0.803468	3.436682e-49	-8.615850e-47
Msa1201240	Msa1454480	0.802955	4.387741e-49	-8.615850e-47
Msa1201240	Msa1460830	0.808889	2.481169e-50	-8.615850e-47
Msa0484780	Msa1201240	0.813541	2.430042e-51	-8.615850e-47
Msa0502880	Msa1201240	0.802496	5.458061e-49	-8.615850e-47
Msa0866550	Msa1201240	0.810489	1.123734e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1201240	MtrunA17_Chr8g0371101	91.270	378	32	1	106	483	1	377	0.0	704
Msa1201240	MtrunA17_Chr8g0371031	76.842	380	84	4	106	483	20	397	0.0	595
Msa1201240	MtrunA17_Chr2g0300541	73.067	401	96	5	86	483	1	392	0.0	560
Msa1201240	MtrunA17_Chr5g0435201	35.027	374	194	12	106	465	1	339	1.55e-50	176
Msa1201240	MtrunA17_Chr6g0488491	34.474	380	192	11	107	469	4	343	1.25e-49	174
Msa1201240	MtrunA17_Chr8g0378641	33.588	393	198	14	101	459	1	364	1.43e-47	169
Msa1201240	MtrunA17_Chr7g0261121	32.682	358	192	11	113	445	15	348	1.01e-43	158
Msa1201240	MtrunA17_Chr1g0157711	30.227	397	222	13	106	481	1	363	1.27e-35	135
Msa1201240	MtrunA17_Chr3g0088831	30.282	426	235	15	104	481	9	420	3.83e-35	135
Msa1201240	MtrunA17_Chr3g0088931	29.156	391	211	14	108	470	2	354	3.65e-30	120
Msa1201240	MtrunA17_Chr6g0457531	29.722	360	189	13	110	445	4	323	2.31e-29	118
Msa1201240	MtrunA17_Chr4g0047461	33.865	251	134	8	240	469	7	246	1.09e-28	114
Msa1201240	MtrunA17_Chr3g0088751	27.456	397	238	11	109	481	3	373	1.13e-27	114
Msa1201240	MtrunA17_Chr1g0146401	27.518	407	225	16	91	464	1	370	2.04e-26	110
Msa1201240	MtrunA17_Chr2g0303111	30.168	358	207	14	106	447	1	331	2.17e-26	110
Msa1201240	MtrunA17_Chr3g0088531	31.680	363	193	16	110	450	2	331	3.01e-25	107
Msa1201240	MtrunA17_Chr6g0460421	27.930	401	235	18	110	483	4	377	3.00e-24	104
Msa1201240	MtrunA17_Chr3g0088721	29.064	406	218	17	103	480	1	364	7.11e-24	103
Msa1201240	MtrunA17_Chr6g0460451	28.180	401	234	18	110	483	4	377	2.07e-23	101
Msa1201240	MtrunA17_Chr3g0089001	29.513	349	195	11	110	447	34	342	3.26e-23	101
Msa1201240	MtrunA17_Chr6g0457541	26.768	396	201	15	108	467	2	344	1.29e-22	99.4
Msa1201240	MtrunA17_Chr3g0088551	26.933	375	217	15	109	447	3	356	1.99e-22	99.0
Msa1201240	MtrunA17_Chr1g0151721	27.747	364	194	11	91	447	1	302	2.51e-22	98.2
Msa1201240	MtrunA17_Chr3g0088951	26.724	348	210	10	109	447	3	314	5.15e-22	97.4
Msa1201240	MtrunA17_Chr3g0088541	29.477	363	191	16	110	450	3	322	1.39e-21	96.3
Msa1201240	MtrunA17_Chr6g0457501	29.086	361	204	17	110	447	4	335	4.47e-21	95.5
Msa1201240	MtrunA17_Chr3g0143621	26.933	401	231	16	110	479	22	391	1.26e-20	94.0
Msa1201240	MtrunA17_Chr3g0088621	25.441	397	239	16	95	459	2	373	1.47e-20	93.6
Msa1201240	MtrunA17_Chr3g0088671	28.134	359	194	16	110	447	4	319	3.85e-20	91.7
Msa1201240	MtrunA17_Chr3g0088841	27.374	358	201	15	111	447	7	326	1.55e-19	90.1
Msa1201240	MtrunA17_Chr3g0088631	23.810	378	228	8	110	456	4	352	7.21e-19	88.6
Msa1201240	MtrunA17_Chr3g0088741	27.824	363	197	16	110	450	4	323	8.45e-19	87.8
Msa1201240	MtrunA17_Chr3g0143611	25.337	371	231	16	110	468	3	339	1.08e-18	87.4
Msa1201240	MtrunA17_Chr3g0088921	27.536	414	208	20	106	464	1	377	2.06e-18	87.0
Msa1201240	MtrunA17_Chr3g0088601	26.027	365	220	9	110	448	16	356	3.07e-18	86.7
Msa1201240	MtrunA17_Chr3g0127311	26.171	363	215	11	109	445	5	340	4.07e-18	86.3
Msa1201240	MtrunA17_Chr3g0088661	28.750	400	165	17	103	475	1	307	2.98e-17	82.8
Msa1201240	MtrunA17_Chr3g0088701	28.500	400	166	17	103	475	1	307	7.07e-17	81.6
Msa1201240	MtrunA17_Chr3g0096401	28.191	376	219	16	111	481	30	359	1.67e-15	78.2
Msa1201240	MtrunA17_Chr3g0092331	36.496	137	81	2	315	447	3	137	2.26e-15	74.7
Msa1201240	MtrunA17_Chr3g0088711	32.203	177	85	6	300	473	17	161	1.07e-14	72.4
Msa1201240	MtrunA17_Chr3g0088561	35.115	131	76	2	315	445	5	126	9.69e-13	66.6
Msa1201240	MtrunA17_Chr3g0088581	27.711	166	107	4	302	459	27	187	2.54e-12	66.2
Msa1201240	MtrunA17_Chr3g0088681	32.143	140	75	3	315	454	5	124	4.75e-12	64.3
Msa1201240	MtrunA17_Chr1g0151701	34.965	143	75	4	312	447	2	133	7.57e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1201240	AT2G17030.1	31.135	379	224	13	110	466	2	365	3.92e-39	147
Msa1201240	AT1G65770.1	30.084	359	217	10	110	459	2	335	3.35e-35	135
Msa1201240	AT4G35733.1	29.365	378	211	12	106	464	1	341	3.67e-34	132
Msa1201240	AT1G44080.1	27.419	372	203	9	109	463	3	324	4.72e-29	117
Msa1201240	AT3G25750.2	28.760	379	205	14	108	461	2	340	1.16e-28	117
Msa1201240	AT2G17036.2	27.614	373	239	11	110	466	1	358	1.49e-28	117
Msa1201240	AT2G17036.1	27.614	373	239	11	110	466	2	359	1.73e-28	117
Msa1201240	AT1G65740.1	29.041	365	225	12	110	459	2	347	2.30e-26	110
Msa1201240	AT3G25750.1	28.117	377	196	13	108	461	2	326	1.41e-25	107
Msa1201240	AT2G26160.1	27.841	352	209	12	108	447	2	320	1.82e-24	104
Msa1201240	AT1G65760.1	28.686	373	216	15	111	464	5	346	3.86e-23	100
Msa1201240	AT2G17690.1	27.316	421	221	14	111	464	3	405	2.70e-21	96.3
Msa1201240	AT2G16290.1	24.615	390	234	16	110	461	2	369	7.79e-18	85.9
Msa1201240	AT1G64840.1	25.989	354	215	13	111	447	4	327	1.89e-15	78.6
Msa1201240	AT5G24040.1	23.499	383	198	17	110	451	2	330	3.06e-13	71.6
Msa1201240	AT1G57906.1	31.638	177	79	8	292	434	58	226	4.73e-12	66.2

Find 125 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTACATTCTGCACTTCTTTA+TGG	0.053179	8_1:+54428258	None:intergenic
TGTAGCTTGTTGTATCTTTC+AGG	0.173014	8_1:-54429441	Msa1201240:CDS
TTCAACAAGTTCCATATAAT+TGG	0.175089	8_1:+54429581	None:intergenic
AGGGAATTGCATCTACTTTA+AGG	0.185868	8_1:-54428976	Msa1201240:CDS
TATGGAACTTGTTGAATTAT+AGG	0.195427	8_1:-54429574	Msa1201240:CDS
AAAGGAAAAGAAGGAGAATT+TGG	0.229744	8_1:+54429839	None:intergenic
GTTTCTGCTAAAGAATTAAT+TGG	0.230909	8_1:-54429005	Msa1201240:CDS
ATCTTGGACCATAGCTCTTT+GGG	0.243195	8_1:+54429947	None:intergenic
GTGAAAAGGAATAATAATTA+AGG	0.251820	8_1:-54428034	Msa1201240:three_prime_UTR
ATCTTTCAGGAAGGTAAATT+AGG	0.270135	8_1:-54429428	Msa1201240:CDS
CTTGTGCCACCACTGATTAC+AGG	0.281043	8_1:-54431431	Msa1201240:intron
TATCTTGGACCATAGCTCTT+TGG	0.291416	8_1:+54429946	None:intergenic
ATGGAACTTGTTGAATTATA+GGG	0.301160	8_1:-54429573	Msa1201240:CDS
CACCCTACGTCTGCCCCGAC+TGG	0.310258	8_1:-54431487	Msa1201240:CDS
TCACCTCCTTTGTGTGGGTT+TGG	0.311829	8_1:-54429251	Msa1201240:CDS
TGAAATATGGAGACACAAAA+TGG	0.318037	8_1:-54429400	Msa1201240:CDS
AAATTAGGATTCATGAAATA+TGG	0.332072	8_1:-54429413	Msa1201240:CDS
ATCAAGATACTTCCCTATCT+TGG	0.333662	8_1:+54429931	None:intergenic
ACAACAGTTCTCCAATTATA+TGG	0.333718	8_1:-54429592	Msa1201240:CDS
TTGATAGAGGTAATTGTATA+GGG	0.347302	8_1:+54429790	None:intergenic
AATAATTAAGGGAACTGATC+AGG	0.349159	8_1:-54428022	Msa1201240:three_prime_UTR
CTCACAAAACTTGATGAGTA+TGG	0.352709	8_1:+54429512	None:intergenic
ACTCTCCTTGGATTAATGTT+GGG	0.357299	8_1:-54429469	Msa1201240:CDS
ATTGATAGAGGTAATTGTAT+AGG	0.365166	8_1:+54429789	None:intergenic
CACTTTGATCAACCAACCTT+TGG	0.369704	8_1:+54429694	None:intergenic
ATTATTATTCCTTGGTGAAA+AGG	0.369932	8_1:-54428048	Msa1201240:three_prime_UTR
AAAGAGCTATGGTCCAAGAT+AGG	0.372622	8_1:-54429944	Msa1201240:CDS
ATAAATGGGGAACTATTTCA+TGG	0.373052	8_1:-54429307	Msa1201240:CDS
CTTGCATTGACAAGACTTTG+TGG	0.374534	8_1:-54428890	Msa1201240:CDS
AGGTAATTGTATAGGGAGAC+AGG	0.376170	8_1:+54429797	None:intergenic
ACATTAATCCAAGGAGAGTT+AGG	0.379494	8_1:+54429473	None:intergenic
AAAGAATTAATTGGATATCA+AGG	0.389911	8_1:-54428996	Msa1201240:CDS
GATAATTTGCAGTCGGAGAT+AGG	0.391629	8_1:+54429632	None:intergenic
TTTATATGTTGGATGAAGAA+TGG	0.404503	8_1:-54429097	Msa1201240:CDS
CCATGATCGGGACGCGGTTG+TGG	0.420452	8_1:-54429132	Msa1201240:CDS
ATTTGGAAGAGAGGGAGGAA+TGG	0.422238	8_1:+54429856	None:intergenic
AAGATGGAACTTGCATTAGT+TGG	0.425401	8_1:+54429746	None:intergenic
ACCCTACGTCTGCCCCGACT+GGG	0.425539	8_1:-54431486	Msa1201240:CDS
AGATGTTAGAGGACATTAAC+AGG	0.426803	8_1:-54428849	Msa1201240:exon
TGTCCTATCCTAACTCTCCT+TGG	0.428803	8_1:-54429481	Msa1201240:CDS
TGATGATGTCATTGTATTCA+AGG	0.430249	8_1:-54429348	Msa1201240:CDS
TTATATGTTGGATGAAGAAT+GGG	0.432756	8_1:-54429096	Msa1201240:CDS
GATAAATAGTTGATTGATAG+AGG	0.439760	8_1:+54429777	None:intergenic
CTCTCCTTGGATTAATGTTG+GGG	0.439895	8_1:-54429468	Msa1201240:CDS
AACATTTGGTGGAATCTTGT+GGG	0.442266	8_1:-54429220	Msa1201240:CDS
GTTTGGAAACAAGAAACATT+TGG	0.451619	8_1:-54429234	Msa1201240:CDS
AACTCTCCTTGGATTAATGT+TGG	0.455668	8_1:-54429470	Msa1201240:CDS
GAAGGAGAATTTGGAAGAGA+GGG	0.458355	8_1:+54429848	None:intergenic
TGTATGTAATTATTATTCCT+TGG	0.461026	8_1:-54428056	Msa1201240:three_prime_UTR
TGATGAAGTGAAAGAGAGAA+AGG	0.463163	8_1:+54431614	None:intergenic
TGAAAAGGAATAATAATTAA+GGG	0.463750	8_1:-54428033	Msa1201240:three_prime_UTR
AAACATTTGGTGGAATCTTG+TGG	0.483180	8_1:-54429221	Msa1201240:CDS
CACGTTGGATGAGATGTTAG+AGG	0.485509	8_1:-54428860	Msa1201240:exon
TGTTGGAAGACGCCATGATC+GGG	0.485748	8_1:-54429144	Msa1201240:CDS
TTATAGGTTTACTTGTGAAA+AGG	0.488114	8_1:-54427985	Msa1201240:three_prime_UTR
TTAGAGGACATTAACAGGTC+TGG	0.489507	8_1:-54428844	Msa1201240:exon
AGAAGGAGAATTTGGAAGAG+AGG	0.489920	8_1:+54429847	None:intergenic
CAAGAAAGGTTGATCGGATA+CGG	0.491225	8_1:+54431454	None:intergenic
CCACAACCGCGTCCCGATCA+TGG	0.492597	8_1:+54429132	None:intergenic
GGTATTTGTAAAGGAAAAGA+AGG	0.498247	8_1:+54429830	None:intergenic
ACAACTGCTGCTTAAAATGA+AGG	0.500009	8_1:+54429037	None:intergenic
CACCTTCTTATACCAAAGGT+TGG	0.500177	8_1:-54429706	Msa1201240:CDS
AGTGGTGGCACAAGCAAGAA+AGG	0.503006	8_1:+54431440	None:intergenic
TAATGCTCGTATGTACAGCT+TGG	0.504456	8_1:-54428946	Msa1201240:CDS
GGAGAATTTGGAAGAGAGGG+AGG	0.512512	8_1:+54429851	None:intergenic
AAATCATGACAGAAGCAAGC+TGG	0.516173	8_1:+54428105	None:intergenic
AACCAACCTTTGGTATAAGA+AGG	0.522904	8_1:+54429704	None:intergenic
GACATTAACAGGTCTGGCAA+TGG	0.526788	8_1:-54428838	Msa1201240:three_prime_UTR
AACATGAGAGGGAACAATGT+TGG	0.526890	8_1:-54429161	Msa1201240:CDS
CCCTACGTCTGCCCCGACTG+GGG	0.527213	8_1:-54431485	Msa1201240:CDS
TTGGTATAAGAAGGTGCTAA+AGG	0.538170	8_1:+54429713	None:intergenic
AGGGAATAGAATTGAAGAAG+AGG	0.544380	8_1:+54431535	None:intergenic
AAGAGCTATGGTCCAAGATA+GGG	0.546977	8_1:-54429943	Msa1201240:CDS
CTCATCTCCCCAAAGAGCTA+TGG	0.548235	8_1:-54429955	Msa1201240:CDS
GATGATGGCGGAAGGACAAA+GGG	0.549094	8_1:+54431516	None:intergenic
ATAATTAAGGGAACTGATCA+GGG	0.550869	8_1:-54428021	Msa1201240:three_prime_UTR
GGACTCAACAAACTCTTCAA+AGG	0.551008	8_1:+54429653	None:intergenic
GTGTTTCAAAGTTTATATGT+TGG	0.555002	8_1:-54429108	Msa1201240:CDS
ATGTTGGAAGACGCCATGAT+CGG	0.555148	8_1:-54429145	Msa1201240:CDS
GGTAATTGTATAGGGAGACA+GGG	0.555775	8_1:+54429798	None:intergenic
TTCTGCACTTCTTTATGGAG+AGG	0.557580	8_1:+54428263	None:intergenic
TAAAGGTGGTAAATTGAAGA+TGG	0.558190	8_1:+54429730	None:intergenic
TGATGATGGCGGAAGGACAA+AGG	0.561064	8_1:+54431515	None:intergenic
AAGAATTAATTGGATATCAA+GGG	0.561141	8_1:-54428995	Msa1201240:CDS
ATGGTGCGAGGTATTTGTAA+AGG	0.569151	8_1:+54429821	None:intergenic
TGGAAACAAGAAACATTTGG+TGG	0.570071	8_1:-54429231	Msa1201240:CDS
TTAGCACCTTCTTATACCAA+AGG	0.570602	8_1:-54429710	Msa1201240:CDS
GAAAGACTTTGTACATCAGA+AGG	0.574062	8_1:-54428157	Msa1201240:three_prime_UTR
CAAAGGTTGGTTGATCAAAG+TGG	0.574189	8_1:-54429693	Msa1201240:CDS
CACAAAGTCTTGTCAATGCA+AGG	0.575490	8_1:+54428891	None:intergenic
GTTGTTGATAGATACTATGA+AGG	0.579405	8_1:-54429188	Msa1201240:CDS
AAAGGTTGATCGGATACGGC+GGG	0.581865	8_1:+54431458	None:intergenic
ATGAAGGTGAAAACATGAGA+GGG	0.582267	8_1:-54429172	Msa1201240:CDS
GATGATGTCATTGTATTCAA+GGG	0.582955	8_1:-54429347	Msa1201240:CDS
CACAAGCAAGAAAGGTTGAT+CGG	0.583350	8_1:+54431448	None:intergenic
AGGATATGATAATTTGCAGT+CGG	0.584721	8_1:+54429625	None:intergenic
CTTTGTGGTCTCATTCACGT+TGG	0.590681	8_1:-54428875	Msa1201240:CDS
TAGAATTTCACCATGTGAGA+CGG	0.590948	8_1:+54431289	None:intergenic
GCTTGTTGTATCTTTCAGGA+AGG	0.596136	8_1:-54429437	Msa1201240:CDS
AATCTTTGAACTGTCAGCCA+TGG	0.599833	8_1:-54429996	Msa1201240:CDS
CCTACGTCTGCCCCGACTGG+GGG	0.602545	8_1:-54431484	Msa1201240:CDS
GTATAAGAAGGTGCTAAAGG+TGG	0.604135	8_1:+54429716	None:intergenic
AAGACGCCATGATCGGGACG+CGG	0.613949	8_1:-54429138	Msa1201240:CDS
CGTAGGGTGATGACTGATGA+TGG	0.621889	8_1:+54431501	None:intergenic
CAATCCCCAACATTAATCCA+AGG	0.626001	8_1:+54429464	None:intergenic
TCTTGGACCATAGCTCTTTG+GGG	0.628895	8_1:+54429948	None:intergenic
GAACTGTTGTTACTGTCAAG+AGG	0.629902	8_1:+54429605	None:intergenic
TATATGTTGGATGAAGAATG+GGG	0.631666	8_1:-54429095	Msa1201240:CDS
GGGAACTGATCAGGGCACAA+TGG	0.634451	8_1:-54428013	Msa1201240:three_prime_UTR
ATTGTATAGGGAGACAGGGA+TGG	0.639587	8_1:+54429802	None:intergenic
AATCCAAGGAGAGTTAGGAT+AGG	0.641071	8_1:+54429478	None:intergenic
GAGACGGTAGATATACAGAC+AGG	0.642951	8_1:+54431305	None:intergenic
AGGGAGACAGGGATGGTGCG+AGG	0.646104	8_1:+54429809	None:intergenic
ATATGTTGGATGAAGAATGG+GGG	0.646805	8_1:-54429094	Msa1201240:CDS
TATGAAGGTGAAAACATGAG+AGG	0.647138	8_1:-54429173	Msa1201240:CDS
GAAAGGTTGATCGGATACGG+CGG	0.649643	8_1:+54431457	None:intergenic
TTTCCAAACCCACACAAAGG+AGG	0.652810	8_1:+54429248	None:intergenic
TGTATATCTACCGTCTCACA+TGG	0.653506	8_1:-54431299	Msa1201240:CDS
AATGCAAGGATCAAAATCAA+CGG	0.653913	8_1:+54428905	None:intergenic
AGACACAAAATGGAAACTTG+TGG	0.657681	8_1:-54429390	Msa1201240:CDS
TTGTTTCCAAACCCACACAA+AGG	0.659541	8_1:+54429245	None:intergenic
TACGTACCTGTAATCAGTGG+TGG	0.665003	8_1:+54431425	None:intergenic
TGATGACTGATGATGGCGGA+AGG	0.680427	8_1:+54431508	None:intergenic
ATATACGTACCTGTAATCAG+TGG	0.711726	8_1:+54431422	None:intergenic
AGGGTGATGACTGATGATGG+CGG	0.815078	8_1:+54431504	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTATTAATTTTTTTTAT+TGG	-	chr8_1:54428459-54428478	Msa1201240:intron	0.0%
!!!	ATTTTTTTTATTTTTTATAT+TGG	-	chr8_1:54428729-54428748	Msa1201240:intron	0.0%
!!!	TTTTTTTTATTTTTTATATT+GGG	-	chr8_1:54428730-54428749	Msa1201240:intron	0.0%
!!!	AAGTTTTTTCTTTTATTATA+GGG	+	chr8_1:54428433-54428452	None:intergenic	10.0%
!!!	AAAGTTTTTTCTTTTATTAT+AGG	+	chr8_1:54428434-54428453	None:intergenic	10.0%
!!	AAAAATAAGTATAATAGAAA+TGG	+	chr8_1:54429531-54429550	None:intergenic	10.0%
!!	ATATAATTAATTATGTTCAA+CGG	+	chr8_1:54431090-54431109	None:intergenic	10.0%
!!	CATTTAAAGGATATATATTA+AGG	+	chr8_1:54428583-54428602	None:intergenic	15.0%
!!	TAAAAAACTTATTATGGTAT+CGG	+	chr8_1:54428710-54428729	None:intergenic	15.0%
!!	ATAAAATTAACATGTCTTTA+TGG	+	chr8_1:54430916-54430935	None:intergenic	15.0%
!!	ATTAATGAATATAACAAACA+AGG	+	chr8_1:54431064-54431083	None:intergenic	15.0%
!!	TGAAAAGGAATAATAATTAA+GGG	-	chr8_1:54431572-54431591	Msa1201240:CDS	15.0%
!!	AAAACAAAAAAAATGACCAA+AGG	+	chr8_1:54428387-54428406	None:intergenic	20.0%
!!!	CTAAATCTATCTATTTTGTA+AGG	+	chr8_1:54428496-54428515	None:intergenic	20.0%
!!	ACAAAATAGATAGATTTAGT+AGG	-	chr8_1:54428497-54428516	Msa1201240:intron	20.0%
!!	CAAAATAGATAGATTTAGTA+GGG	-	chr8_1:54428498-54428517	Msa1201240:intron	20.0%
!!	AATAATTGGTCATCATTTAA+AGG	+	chr8_1:54428596-54428615	None:intergenic	20.0%
!!!	AATCAAGTATTGCAAATAAT+TGG	+	chr8_1:54428610-54428629	None:intergenic	20.0%
!!	ACTAACTCAACAATTATTAT+TGG	+	chr8_1:54428854-54428873	None:intergenic	20.0%
!!!	CTTCAATGTAGTTTTATAAA+TGG	+	chr8_1:54428994-54429013	None:intergenic	20.0%
!!!	TGTTTTCATGTATTACTTTT+AGG	-	chr8_1:54429178-54429197	Msa1201240:CDS	20.0%
!!!	GTTTTCATGTATTACTTTTA+GGG	-	chr8_1:54429179-54429198	Msa1201240:CDS	20.0%
!!	AATTCAAAAGAATATGCTAA+GGG	+	chr8_1:54429278-54429297	None:intergenic	20.0%
!!	AAATTCAAAAGAATATGCTA+AGG	+	chr8_1:54429279-54429298	None:intergenic	20.0%
!!	TCTATTAATGAATTAGAAAG+TGG	+	chr8_1:54429325-54429344	None:intergenic	20.0%
!!	AAATTAGGATTCATGAAATA+TGG	-	chr8_1:54430192-54430211	Msa1201240:intron	20.0%
!!!	AGTTTTATGTTACTGATAAA+TGG	-	chr8_1:54430283-54430302	Msa1201240:intron	20.0%
!!!	GTTTTATGTTACTGATAAAT+GGG	-	chr8_1:54430284-54430303	Msa1201240:intron	20.0%
!!!	TTTTATGTTACTGATAAATG+GGG	-	chr8_1:54430285-54430304	Msa1201240:intron	20.0%
!!	AAAGAATTAATTGGATATCA+AGG	-	chr8_1:54430609-54430628	Msa1201240:intron	20.0%
!!	AAGAATTAATTGGATATCAA+GGG	-	chr8_1:54430610-54430629	Msa1201240:intron	20.0%
!!!	GACATGTTAATTTTATTACA+TGG	-	chr8_1:54430920-54430939	Msa1201240:intron	20.0%
!!!	GAAAAATATCATTTTGATAC+AGG	+	chr8_1:54431220-54431239	None:intergenic	20.0%
!!	TAAAATATCCAATTATCGAA+AGG	+	chr8_1:54431253-54431272	None:intergenic	20.0%
!!	TGTATGTAATTATTATTCCT+TGG	-	chr8_1:54431549-54431568	Msa1201240:CDS	20.0%
!!	GTGAAAAGGAATAATAATTA+AGG	-	chr8_1:54431571-54431590	Msa1201240:CDS	20.0%
!!!	ATTTTTTTCACTCTCCAAAT+TGG	-	chr8_1:54428221-54428240	Msa1201240:three_prime_UTR	25.0%
!!!	TTTTTTTCACTCTCCAAATT+GGG	-	chr8_1:54428222-54428241	Msa1201240:three_prime_UTR	25.0%
!	ATAGAAAACTGCAGAAAAAA+TGG	+	chr8_1:54429109-54429128	None:intergenic	25.0%
!!	GAATTAGAAAGTGGTATTTT+TGG	+	chr8_1:54429316-54429335	None:intergenic	25.0%
!	AACCAACTAACATATCAAAA+TGG	-	chr8_1:54429476-54429495	Msa1201240:CDS	25.0%
!!!	AACCATTTTGATATGTTAGT+TGG	+	chr8_1:54429481-54429500	None:intergenic	25.0%
!	ATGGAAAAAAAAAACAGAGA+AGG	+	chr8_1:54429512-54429531	None:intergenic	25.0%
!	TTGATAGAGGTAATTGTATA+GGG	+	chr8_1:54429818-54429837	None:intergenic	25.0%
!	ATTGATAGAGGTAATTGTAT+AGG	+	chr8_1:54429819-54429838	None:intergenic	25.0%
!	GATAAATAGTTGATTGATAG+AGG	+	chr8_1:54429831-54429850	None:intergenic	25.0%
!	TTCAACAAGTTCCATATAAT+TGG	+	chr8_1:54430027-54430046	None:intergenic	25.0%
!!!	TATGGAACTTGTTGAATTAT+AGG	-	chr8_1:54430031-54430050	Msa1201240:CDS	25.0%
!!!	ATGGAACTTGTTGAATTATA+GGG	-	chr8_1:54430032-54430051	Msa1201240:intron	25.0%
!	GTGTTTCAAAGTTTATATGT+TGG	-	chr8_1:54430497-54430516	Msa1201240:intron	25.0%
!	TTTATATGTTGGATGAAGAA+TGG	-	chr8_1:54430508-54430527	Msa1201240:intron	25.0%
!	TTATATGTTGGATGAAGAAT+GGG	-	chr8_1:54430509-54430528	Msa1201240:intron	25.0%
!	GTTTCTGCTAAAGAATTAAT+TGG	-	chr8_1:54430600-54430619	Msa1201240:intron	25.0%
!	TGTAATGCAGTATAAGTATT+TGG	-	chr8_1:54431001-54431020	Msa1201240:intron	25.0%
!	ATTTGGTTAATTGTCTTCAA+TGG	-	chr8_1:54431018-54431037	Msa1201240:intron	25.0%
!	CTATTCATTTCTTCATTACA+TGG	+	chr8_1:54431127-54431146	None:intergenic	25.0%
!	TATTTATGCCTTTCGATAAT+TGG	-	chr8_1:54431242-54431261	Msa1201240:intron	25.0%
!!!	AATTAAACTGTGTAGTTTTG+AGG	-	chr8_1:54431423-54431442	Msa1201240:intron	25.0%
!	ATTATTATTCCTTGGTGAAA+AGG	-	chr8_1:54431557-54431576	Msa1201240:CDS	25.0%
!!	ATTATTATTCCTTTTCACCA+AGG	+	chr8_1:54431569-54431588	None:intergenic	25.0%
!	TTATAGGTTTACTTGTGAAA+AGG	-	chr8_1:54431620-54431639	Msa1201240:CDS	25.0%
!!!	TTTTTTCACTCTCCAAATTG+GGG	-	chr8_1:54428223-54428242	Msa1201240:three_prime_UTR	30.0%
	GTTACTAGATCATCCAATTA+AGG	-	chr8_1:54428348-54428367	Msa1201240:intron	30.0%
	AAAAAAAATGACCAAAGGCT+AGG	+	chr8_1:54428382-54428401	None:intergenic	30.0%
	ACACATGAATAGAAAACAAG+TGG	+	chr8_1:54428553-54428572	None:intergenic	30.0%
	AAATTCATGCTTACAAAACC+TGG	-	chr8_1:54428755-54428774	Msa1201240:intron	30.0%
	TCACTCTTTATAAACTTCAG+TGG	-	chr8_1:54428795-54428814	Msa1201240:intron	30.0%
	TGTCACATAGATTAAAGACA+AGG	+	chr8_1:54428821-54428840	None:intergenic	30.0%
	TCTCAATTTCACGTTCAAAT+TGG	-	chr8_1:54428913-54428932	Msa1201240:CDS	30.0%
	TGGATTTCTATTAGCATGTT+TGG	+	chr8_1:54428974-54428993	None:intergenic	30.0%
!	GGTTGCAGTAAAAAATTTTG+TGG	+	chr8_1:54429082-54429101	None:intergenic	30.0%
!	TCAAGTTTGAATCTGGTTTA+TGG	-	chr8_1:54429373-54429392	Msa1201240:CDS	30.0%
!	TTGAATCTGGTTTATGGTTA+AGG	-	chr8_1:54429379-54429398	Msa1201240:CDS	30.0%
!	TGAATCTGGTTTATGGTTAA+GGG	-	chr8_1:54429380-54429399	Msa1201240:CDS	30.0%
	GACTTTAAATGTCTTCTAAG+TGG	+	chr8_1:54429421-54429440	None:intergenic	30.0%
	ATACCTGTGAATTTACAAGA+AGG	+	chr8_1:54429561-54429580	None:intergenic	30.0%
	AAAGGAAAAGAAGGAGAATT+TGG	+	chr8_1:54429769-54429788	None:intergenic	30.0%
	GGTATTTGTAAAGGAAAAGA+AGG	+	chr8_1:54429778-54429797	None:intergenic	30.0%
	TAAAGGTGGTAAATTGAAGA+TGG	+	chr8_1:54429878-54429897	None:intergenic	30.0%
!	AGGATATGATAATTTGCAGT+CGG	+	chr8_1:54429983-54430002	None:intergenic	30.0%
	ACAACAGTTCTCCAATTATA+TGG	-	chr8_1:54430013-54430032	Msa1201240:CDS	30.0%
	ATCTTTCAGGAAGGTAAATT+AGG	-	chr8_1:54430177-54430196	Msa1201240:intron	30.0%
	TGAAATATGGAGACACAAAA+TGG	-	chr8_1:54430205-54430224	Msa1201240:intron	30.0%
!	TGATGATGTCATTGTATTCA+AGG	-	chr8_1:54430257-54430276	Msa1201240:intron	30.0%
	GATGATGTCATTGTATTCAA+GGG	-	chr8_1:54430258-54430277	Msa1201240:intron	30.0%
	ATAAATGGGGAACTATTTCA+TGG	-	chr8_1:54430298-54430317	Msa1201240:intron	30.0%
	GTTTGGAAACAAGAAACATT+TGG	-	chr8_1:54430371-54430390	Msa1201240:intron	30.0%
!	GTTGTTGATAGATACTATGA+AGG	-	chr8_1:54430417-54430436	Msa1201240:intron	30.0%
	TATATGTTGGATGAAGAATG+GGG	-	chr8_1:54430510-54430529	Msa1201240:intron	30.0%
	AATGCAAGGATCAAAATCAA+CGG	+	chr8_1:54430703-54430722	None:intergenic	30.0%
!!!	TGGAGTTTTAAAGGTACATT+TGG	-	chr8_1:54430787-54430806	Msa1201240:intron	30.0%
!!!	GCTATGTTATTTTTGTTTCG+CGG	-	chr8_1:54431382-54431401	Msa1201240:intron	30.0%
	AATAATTAAGGGAACTGATC+AGG	-	chr8_1:54431583-54431602	Msa1201240:CDS	30.0%
	ATAATTAAGGGAACTGATCA+GGG	-	chr8_1:54431584-54431603	Msa1201240:CDS	30.0%
	TGATGAAGTGAAAGAGAGAA+AGG	+	chr8_1:54427994-54428013	None:intergenic	35.0%
	AGGGAATAGAATTGAAGAAG+AGG	+	chr8_1:54428073-54428092	None:intergenic	35.0%
	ATATACGTACCTGTAATCAG+TGG	+	chr8_1:54428186-54428205	None:intergenic	35.0%
!	AGATTTGAGATACCCCAATT+TGG	+	chr8_1:54428238-54428257	None:intergenic	35.0%
	TAGAATTTCACCATGTGAGA+CGG	+	chr8_1:54428319-54428338	None:intergenic	35.0%
!	AAACCTGGCTTGTAAAATGA+CGG	-	chr8_1:54428770-54428789	Msa1201240:intron	35.0%
	TTGTTGAGTTAGTGTCTGTT+TGG	-	chr8_1:54428861-54428880	Msa1201240:exon	35.0%
	TTTCACGTTCAAATTGGCAT+TGG	-	chr8_1:54428919-54428938	Msa1201240:CDS	35.0%
	AACTGCAGAAAAAATGGTTG+TGG	+	chr8_1:54429103-54429122	None:intergenic	35.0%
!	TCTGCAGTTTTCTATTCAAG+AGG	-	chr8_1:54429114-54429133	Msa1201240:CDS	35.0%
!	TTGATAATTCAAACGCAGTG+AGG	-	chr8_1:54429139-54429158	Msa1201240:CDS	35.0%
!!!	TGTGTTTGCAAGTTGGTTTT+TGG	-	chr8_1:54429202-54429221	Msa1201240:CDS	35.0%
!!!	GAAAGTGGTATTTTTGGTGT+TGG	+	chr8_1:54429310-54429329	None:intergenic	35.0%
	TCCTAAGAAAGAACCTATGA+TGG	-	chr8_1:54429447-54429466	Msa1201240:CDS	35.0%
	TCCATCATAGGTTCTTTCTT+AGG	+	chr8_1:54429451-54429470	None:intergenic	35.0%
	TCACCTTCTTGTAAATTCAC+AGG	-	chr8_1:54429555-54429574	Msa1201240:CDS	35.0%
!!!	CCAATCTACTTTTTCATCCA+TGG	+	chr8_1:54429629-54429648	None:intergenic	35.0%
	CCATGGATGAAAAAGTAGAT+TGG	-	chr8_1:54429626-54429645	Msa1201240:CDS	35.0%
	ATCAAGATACTTCCCTATCT+TGG	+	chr8_1:54429677-54429696	None:intergenic	35.0%
	AAGATGGAACTTGCATTAGT+TGG	+	chr8_1:54429862-54429881	None:intergenic	35.0%
!!	TTGGTATAAGAAGGTGCTAA+AGG	+	chr8_1:54429895-54429914	None:intergenic	35.0%
	TTAGCACCTTCTTATACCAA+AGG	-	chr8_1:54429895-54429914	Msa1201240:CDS	35.0%
	AACCAACCTTTGGTATAAGA+AGG	+	chr8_1:54429904-54429923	None:intergenic	35.0%
	CTCACAAAACTTGATGAGTA+TGG	+	chr8_1:54430096-54430115	None:intergenic	35.0%
	ACATTAATCCAAGGAGAGTT+AGG	+	chr8_1:54430135-54430154	None:intergenic	35.0%
	AACTCTCCTTGGATTAATGT+TGG	-	chr8_1:54430135-54430154	Msa1201240:intron	35.0%
	ACTCTCCTTGGATTAATGTT+GGG	-	chr8_1:54430136-54430155	Msa1201240:intron	35.0%
!!	TGTAGCTTGTTGTATCTTTC+AGG	-	chr8_1:54430164-54430183	Msa1201240:intron	35.0%
	AGACACAAAATGGAAACTTG+TGG	-	chr8_1:54430215-54430234	Msa1201240:intron	35.0%
!	AATTTTCACCTCCTTTGTGT+GGG	-	chr8_1:54430349-54430368	Msa1201240:intron	35.0%
	TGGAAACAAGAAACATTTGG+TGG	-	chr8_1:54430374-54430393	Msa1201240:intron	35.0%
!	AAACATTTGGTGGAATCTTG+TGG	-	chr8_1:54430384-54430403	Msa1201240:intron	35.0%
!	AACATTTGGTGGAATCTTGT+GGG	-	chr8_1:54430385-54430404	Msa1201240:intron	35.0%
	TATGAAGGTGAAAACATGAG+AGG	-	chr8_1:54430432-54430451	Msa1201240:intron	35.0%
	ATGAAGGTGAAAACATGAGA+GGG	-	chr8_1:54430433-54430452	Msa1201240:intron	35.0%
	ATATGTTGGATGAAGAATGG+GGG	-	chr8_1:54430511-54430530	Msa1201240:intron	35.0%
!	ACAACTGCTGCTTAAAATGA+AGG	+	chr8_1:54430571-54430590	None:intergenic	35.0%
	AGGGAATTGCATCTACTTTA+AGG	-	chr8_1:54430629-54430648	Msa1201240:intron	35.0%
	AGATGTTAGAGGACATTAAC+AGG	-	chr8_1:54430756-54430775	Msa1201240:intron	35.0%
!	TCCCATCTTTTTATCTGTCT+TGG	-	chr8_1:54430818-54430837	Msa1201240:intron	35.0%
	GCCAAGACAGATAAAAAGAT+GGG	+	chr8_1:54430822-54430841	None:intergenic	35.0%
	TGCCAAGACAGATAAAAAGA+TGG	+	chr8_1:54430823-54430842	None:intergenic	35.0%
!	TTTTATCTGTCTTGGCAATG+TGG	-	chr8_1:54430826-54430845	Msa1201240:intron	35.0%
!!!	TCTTGGCAATGTGGTTTTAT+CGG	-	chr8_1:54430835-54430854	Msa1201240:intron	35.0%
	GGTTAATTGTCTTCAATGGA+TGG	-	chr8_1:54431022-54431041	Msa1201240:intron	35.0%
	CTACATTCTGCACTTCTTTA+TGG	+	chr8_1:54431350-54431369	None:intergenic	35.0%
	GAAAGACTTTGTACATCAGA+AGG	-	chr8_1:54431448-54431467	Msa1201240:CDS	35.0%
!!!	GGGCACAATGGTTTTTTTAT+AGG	-	chr8_1:54431604-54431623	Msa1201240:CDS	35.0%
!	CAAGAAAGGTTGATCGGATA+CGG	+	chr8_1:54428154-54428173	None:intergenic	40.0%
!	CACAAGCAAGAAAGGTTGAT+CGG	+	chr8_1:54428160-54428179	None:intergenic	40.0%
	GTTTCAATCACACACTTTGC+AGG	-	chr8_1:54428276-54428295	Msa1201240:three_prime_UTR	40.0%
	TGTATATCTACCGTCTCACA+TGG	-	chr8_1:54428306-54428325	Msa1201240:intron	40.0%
!	CTAGGAGGGTATACCTTAAT+TGG	+	chr8_1:54428364-54428383	None:intergenic	40.0%
	AAAATGACCAAAGGCTAGGA+GGG	+	chr8_1:54428378-54428397	None:intergenic	40.0%
	AAAAATGACCAAAGGCTAGG+AGG	+	chr8_1:54428379-54428398	None:intergenic	40.0%
!	CTTATTATGGTATCGGAGTC+CGG	+	chr8_1:54428703-54428722	None:intergenic	40.0%
!	AACCTGGCTTGTAAAATGAC+GGG	-	chr8_1:54428771-54428790	Msa1201240:intron	40.0%
!	TGAGACATGCGTTTTTAACG+TGG	+	chr8_1:54428898-54428917	None:intergenic	40.0%
!	TTGGCATTGGAATGTGAGTT+TGG	-	chr8_1:54428932-54428951	Msa1201240:CDS	40.0%
	TTCAAACGCAGTGAGGATTT+AGG	-	chr8_1:54429146-54429165	Msa1201240:CDS	40.0%
!	TTTAGGGTGTGTTTGCAAGT+TGG	-	chr8_1:54429195-54429214	Msa1201240:CDS	40.0%
!	CAAACTTGAGCGTTGTTTAG+AGG	+	chr8_1:54429362-54429381	None:intergenic	40.0%
	ACAACGCTCAAGTTTGAATC+TGG	-	chr8_1:54429366-54429385	Msa1201240:CDS	40.0%
	GTTGGTTGTGTTTCCATCAT+AGG	+	chr8_1:54429463-54429482	None:intergenic	40.0%
	AATCTTTGAACTGTCAGCCA+TGG	-	chr8_1:54429609-54429628	Msa1201240:CDS	40.0%
	ATCTTGGACCATAGCTCTTT+GGG	+	chr8_1:54429661-54429680	None:intergenic	40.0%
	TATCTTGGACCATAGCTCTT+TGG	+	chr8_1:54429662-54429681	None:intergenic	40.0%
	AAAGAGCTATGGTCCAAGAT+AGG	-	chr8_1:54429661-54429680	Msa1201240:CDS	40.0%
	AAGAGCTATGGTCCAAGATA+GGG	-	chr8_1:54429662-54429681	Msa1201240:CDS	40.0%
	GAAGGAGAATTTGGAAGAGA+GGG	+	chr8_1:54429760-54429779	None:intergenic	40.0%
	AGAAGGAGAATTTGGAAGAG+AGG	+	chr8_1:54429761-54429780	None:intergenic	40.0%
!	ATGGTGCGAGGTATTTGTAA+AGG	+	chr8_1:54429787-54429806	None:intergenic	40.0%
	GGTAATTGTATAGGGAGACA+GGG	+	chr8_1:54429810-54429829	None:intergenic	40.0%
	AGGTAATTGTATAGGGAGAC+AGG	+	chr8_1:54429811-54429830	None:intergenic	40.0%
!!	GTATAAGAAGGTGCTAAAGG+TGG	+	chr8_1:54429892-54429911	None:intergenic	40.0%
	CACCTTCTTATACCAAAGGT+TGG	-	chr8_1:54429899-54429918	Msa1201240:CDS	40.0%
	CACTTTGATCAACCAACCTT+TGG	+	chr8_1:54429914-54429933	None:intergenic	40.0%
!	CAAAGGTTGGTTGATCAAAG+TGG	-	chr8_1:54429912-54429931	Msa1201240:CDS	40.0%
	GGACTCAACAAACTCTTCAA+AGG	+	chr8_1:54429955-54429974	None:intergenic	40.0%
!	GATAATTTGCAGTCGGAGAT+AGG	+	chr8_1:54429976-54429995	None:intergenic	40.0%
	GAACTGTTGTTACTGTCAAG+AGG	+	chr8_1:54430003-54430022	None:intergenic	40.0%
	AATCCAAGGAGAGTTAGGAT+AGG	+	chr8_1:54430130-54430149	None:intergenic	40.0%
	CTCTCCTTGGATTAATGTTG+GGG	-	chr8_1:54430137-54430156	Msa1201240:intron	40.0%
	CAATCCCCAACATTAATCCA+AGG	+	chr8_1:54430144-54430163	None:intergenic	40.0%
!!	GCTTGTTGTATCTTTCAGGA+AGG	-	chr8_1:54430168-54430187	Msa1201240:intron	40.0%
!	CAATTTTCACCTCCTTTGTG+TGG	-	chr8_1:54430348-54430367	Msa1201240:intron	40.0%
	TTGTTTCCAAACCCACACAA+AGG	+	chr8_1:54430363-54430382	None:intergenic	40.0%
	AACATGAGAGGGAACAATGT+TGG	-	chr8_1:54430444-54430463	Msa1201240:intron	40.0%
	TAATGCTCGTATGTACAGCT+TGG	-	chr8_1:54430659-54430678	Msa1201240:intron	40.0%
!	CACAAAGTCTTGTCAATGCA+AGG	+	chr8_1:54430717-54430736	None:intergenic	40.0%
!	CTTGCATTGACAAGACTTTG+TGG	-	chr8_1:54430715-54430734	Msa1201240:intron	40.0%
	TTAGAGGACATTAACAGGTC+TGG	-	chr8_1:54430761-54430780	Msa1201240:intron	40.0%
!!	GTCTGGCAATGGAGTTTTAA+AGG	-	chr8_1:54430778-54430797	Msa1201240:intron	40.0%
	GATTCATTGCGTTCAGTAGA+TGG	-	chr8_1:54430963-54430982	Msa1201240:intron	40.0%
	TGCGTTCAGTAGATGGTTAT+TGG	-	chr8_1:54430970-54430989	Msa1201240:intron	40.0%
	GCGTTCAGTAGATGGTTATT+GGG	-	chr8_1:54430971-54430990	Msa1201240:intron	40.0%
!!	AAGCACATCAGTACTGATAC+AGG	+	chr8_1:54431281-54431300	None:intergenic	40.0%
	ATCTAGACGCTCTTAACTGT+TGG	+	chr8_1:54431311-54431330	None:intergenic	40.0%
	TTCTGCACTTCTTTATGGAG+AGG	+	chr8_1:54431345-54431364	None:intergenic	40.0%
	AAATCATGACAGAAGCAAGC+TGG	+	chr8_1:54431503-54431522	None:intergenic	40.0%
	TACGTACCTGTAATCAGTGG+TGG	+	chr8_1:54428183-54428202	None:intergenic	45.0%
	GAGACGGTAGATATACAGAC+AGG	+	chr8_1:54428303-54428322	None:intergenic	45.0%
	TCAACAATGTTGTGCACGAG+GGG	+	chr8_1:54428645-54428664	None:intergenic	45.0%
	CTCAACAATGTTGTGCACGA+GGG	+	chr8_1:54428646-54428665	None:intergenic	45.0%
	GTGCACAACATTGTTGAGCT+TGG	-	chr8_1:54428648-54428667	Msa1201240:intron	45.0%
	TTGGTGCGTGTATGTATGGT+GGG	-	chr8_1:54428667-54428686	Msa1201240:intron	45.0%
!	ACCTGGCTTGTAAAATGACG+GGG	-	chr8_1:54428772-54428791	Msa1201240:intron	45.0%
	TCTTGGACCATAGCTCTTTG+GGG	+	chr8_1:54429660-54429679	None:intergenic	45.0%
	ATTTGGAAGAGAGGGAGGAA+TGG	+	chr8_1:54429752-54429771	None:intergenic	45.0%
	ATTGTATAGGGAGACAGGGA+TGG	+	chr8_1:54429806-54429825	None:intergenic	45.0%
	TGTCCTATCCTAACTCTCCT+TGG	-	chr8_1:54430124-54430143	Msa1201240:intron	45.0%
	TTTCCAAACCCACACAAAGG+AGG	+	chr8_1:54430360-54430379	None:intergenic	45.0%
	ATGTTGGAAGACGCCATGAT+CGG	-	chr8_1:54430460-54430479	Msa1201240:intron	45.0%
	TGGATGAAGAATGGGGGAAA+TGG	-	chr8_1:54430517-54430536	Msa1201240:intron	45.0%
	GGATGAAGAATGGGGGAAAT+GGG	-	chr8_1:54430518-54430537	Msa1201240:intron	45.0%
	CTTTGTGGTCTCATTCACGT+TGG	-	chr8_1:54430730-54430749	Msa1201240:intron	45.0%
	CACGTTGGATGAGATGTTAG+AGG	-	chr8_1:54430745-54430764	Msa1201240:intron	45.0%
	GACATTAACAGGTCTGGCAA+TGG	-	chr8_1:54430767-54430786	Msa1201240:intron	45.0%
!!	AAAAAATAAAAAACTTATTA+TGG	+	chr8_1:54428716-54428735	None:intergenic	5.0%
!!	GATGATGGCGGAAGGACAAA+GGG	+	chr8_1:54428092-54428111	None:intergenic	50.0%
!!	TGATGATGGCGGAAGGACAA+AGG	+	chr8_1:54428093-54428112	None:intergenic	50.0%
!!	TGATGACTGATGATGGCGGA+AGG	+	chr8_1:54428100-54428119	None:intergenic	50.0%
!!	AGGGTGATGACTGATGATGG+CGG	+	chr8_1:54428104-54428123	None:intergenic	50.0%
	CGTAGGGTGATGACTGATGA+TGG	+	chr8_1:54428107-54428126	None:intergenic	50.0%
!	AAAGGTTGATCGGATACGGC+GGG	+	chr8_1:54428150-54428169	None:intergenic	50.0%
!	GAAAGGTTGATCGGATACGG+CGG	+	chr8_1:54428151-54428170	None:intergenic	50.0%
	AGTGGTGGCACAAGCAAGAA+AGG	+	chr8_1:54428168-54428187	None:intergenic	50.0%
!	CTTGTGCCACCACTGATTAC+AGG	-	chr8_1:54428174-54428193	Msa1201240:three_prime_UTR	50.0%
!	AGGTATACCCTCCTAGCCTT+TGG	-	chr8_1:54428368-54428387	Msa1201240:intron	50.0%
	TTGTGCACGAGGGGATCAAT+TGG	+	chr8_1:54428636-54428655	None:intergenic	50.0%
	GCTCAACAATGTTGTGCACG+AGG	+	chr8_1:54428647-54428666	None:intergenic	50.0%
!!	GAGCTTGGTGCGTGTATGTA+TGG	-	chr8_1:54428663-54428682	Msa1201240:intron	50.0%
!	CTTGGTGCGTGTATGTATGG+TGG	-	chr8_1:54428666-54428685	Msa1201240:intron	50.0%
!	ATGGTATCGGAGTCCGGTTT+CGG	+	chr8_1:54428697-54428716	None:intergenic	50.0%
!!	ACCCCGTCATTTTACAAGCC+AGG	+	chr8_1:54428776-54428795	None:intergenic	50.0%
	CTCATCTCCCCAAAGAGCTA+TGG	-	chr8_1:54429650-54429669	Msa1201240:CDS	50.0%
	GGAGAATTTGGAAGAGAGGG+AGG	+	chr8_1:54429757-54429776	None:intergenic	50.0%
	TCACCTCCTTTGTGTGGGTT+TGG	-	chr8_1:54430354-54430373	Msa1201240:intron	50.0%
	TGTTGGAAGACGCCATGATC+GGG	-	chr8_1:54430461-54430480	Msa1201240:intron	50.0%
!!	TATGGTGGGTGACCCGAAAC+CGG	-	chr8_1:54428681-54428700	Msa1201240:intron	55.0%
!	TGGTATCGGAGTCCGGTTTC+GGG	+	chr8_1:54428696-54428715	None:intergenic	55.0%
	GGGAACTGATCAGGGCACAA+TGG	-	chr8_1:54431592-54431611	Msa1201240:CDS	55.0%
	AAGACGCCATGATCGGGACG+CGG	-	chr8_1:54430467-54430486	Msa1201240:intron	60.0%
	ACCCTACGTCTGCCCCGACT+GGG	-	chr8_1:54428119-54428138	Msa1201240:three_prime_UTR	65.0%
	AGGGAGACAGGGATGGTGCG+AGG	+	chr8_1:54429799-54429818	None:intergenic	65.0%
	CCACAACCGCGTCCCGATCA+TGG	+	chr8_1:54430476-54430495	None:intergenic	65.0%
	CCATGATCGGGACGCGGTTG+TGG	-	chr8_1:54430473-54430492	Msa1201240:intron	65.0%
	CACCCTACGTCTGCCCCGAC+TGG	-	chr8_1:54428118-54428137	Msa1201240:three_prime_UTR	70.0%
	CCCCAGTCGGGGCAGACGTA+GGG	+	chr8_1:54428123-54428142	None:intergenic	70.0%
	CCCTACGTCTGCCCCGACTG+GGG	-	chr8_1:54428120-54428139	Msa1201240:three_prime_UTR	70.0%
	CCTACGTCTGCCCCGACTGG+GGG	-	chr8_1:54428121-54428140	Msa1201240:three_prime_UTR	70.0%
	TACGGCGGGATGCCCCCAGT+CGG	+	chr8_1:54428136-54428155	None:intergenic	70.0%
	CCCCCAGTCGGGGCAGACGT+AGG	+	chr8_1:54428124-54428143	None:intergenic	75.0%
	ACGGCGGGATGCCCCCAGTC+GGG	+	chr8_1:54428135-54428154	None:intergenic	75.0%
!	CGGCGGGATGCCCCCAGTCG+GGG	+	chr8_1:54428134-54428153	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	54427982	54431645	54427982	ID=Msa1201240;Name=Msa1201240
chr8_1	mRNA	54427982	54431645	54427982	ID=Msa1201240-mRNA-1;Parent=Msa1201240;Name=Msa1201240-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0\|0.66\|0.5\|1\|0.33\|0.25\|4\|366\|483
chr8_1	exon	54427982	54428312	54427982	ID=Msa1201240-mRNA-1:exon:17469;Parent=Msa1201240-mRNA-1
chr8_1	exon	54428828	54430050	54428828	ID=Msa1201240-mRNA-1:exon:17468;Parent=Msa1201240-mRNA-1
chr8_1	exon	54431280	54431329	54431280	ID=Msa1201240-mRNA-1:exon:17467;Parent=Msa1201240-mRNA-1
chr8_1	exon	54431432	54431645	54431432	ID=Msa1201240-mRNA-1:exon:17466;Parent=Msa1201240-mRNA-1
chr8_1	CDS	54431432	54431645	54431432	ID=Msa1201240-mRNA-1:cds;Parent=Msa1201240-mRNA-1
chr8_1	CDS	54431280	54431329	54431280	ID=Msa1201240-mRNA-1:cds;Parent=Msa1201240-mRNA-1
chr8_1	CDS	54428863	54430050	54428863	ID=Msa1201240-mRNA-1:cds;Parent=Msa1201240-mRNA-1
chr8_1	three_prime_UTR	54428828	54428862	54428828	ID=Msa1201240-mRNA-1:three_prime_utr;Parent=Msa1201240-mRNA-1
chr8_1	three_prime_UTR	54427982	54428312	54427982	ID=Msa1201240-mRNA-1:three_prime_utr;Parent=Msa1201240-mRNA-1

Gene Sequence

>Msa1201240

TTTCTCTCACCTTTCTCTCTTTCACTTCATCATCTATATTTTCATAACACTTTCTTTTTTCTTTTCTTTTCTCTTCATCATCTATATTCCTCTTCTTCAATTCTATTCCCTTTGTCCTTCCGCCATCATCAGTCATCACCCTACGTCTGCCCCGACTGGGGGCATCCCGCCGTATCCGATCAACCTTTCTTGCTTGTGCCACCACTGATTACAGGTCCTGTCTGTATATCTACCGTCTCACATGGTGAAATTCTAATGTTCATTGTATTTTTGACATACAAAAAAAAAAAAACACAAATCTTTGAACTGTCAGCCATGGATGAAAAAGTAGATTGGTCTCATCTCCCCAAAGAGCTATGGTCCAAGATAGGGAAGTATCTTGATAATCACATAGATATTCTCAGATTTCGCAGTGTTTGTGAACTATTTCATTCTTCCATTCCTCCCTCTCTTCCAAATTCTCCTTCTTTTCCTTTACAAATACCTCGCACCATCCCTGTCTCCCTATACAATTACCTCTATCAATCAACTATTTATCTTATTGAACCAACTAATGCAAGTTCCATCTTCAATTTACCACCTTTAGCACCTTCTTATACCAAAGGTTGGTTGATCAAAGTGGAAGAATCAAAGAATAATCCTTTGAAGAGTTTGTTGAGTCCTATCTCCGACTGCAAATTATCATATCCTCTTGACAGTAACAACAGTTCTCCAATTATATGGAACTTGTTGAATTATAGGGTCATTGAGCTATGCAAGTCATATTCATTTCAAAAGACACCATACTCATCAAGTTTTGTGAGCAAAGTTGTGTCCTATCCTAACTCTCCTTGGATTAATGTTGGGGATTGTGTAGCTTGTTGTATCTTTCAGGAAGGTAAATTAGGATTCATGAAATATGGAGACACAAAATGGAAACTTGTGGATGAAAACAATTTCTACTATGATGATGTCATTGTATTCAAGGGTCAGTTTTATGTTACTGATAAATGGGGAACTATTTCATGGATTAATATTTCTTCTTTGAAGTTGATACAATTTTCACCTCCTTTGTGTGGGTTTGGAAACAAGAAACATTTGGTGGAATCTTGTGGGAATCTTTATGTTGTTGATAGATACTATGAAGGTGAAAACATGAGAGGGAACAATGTTGGAAGACGCCATGATCGGGACGCGGTTGTGGAGTGTTTCAAAGTTTATATGTTGGATGAAGAATGGGGGAAATGGGTTGATGTGAAAAACTTGAGAGATAGAGCCTTCATTTTAAGCAGCAGTTGTAATTTCTCAGTTTCTGCTAAAGAATTAATTGGATATCAAGGGAATTGCATCTACTTTAAGGATACTTTTAATGCTCGTATGTACAGCTTGGATGATAATATAATCATTACCGTTGATTTTGATCCTTGCATTGACAAGACTTTGTGGTCTCATTCACGTTGGATGAGATGTTAG

Protein sequence

>Msa1201240

FLSPFSLSLHHLYFHNTFFFLFFSLHHLYSSSSILFPLSFRHHQSSPYVCPDWGHPAVSDQPFLLVPPLITGPVCISTVSHGEILMFIVFLTYKKKKTQIFELSAMDEKVDWSHLPKELWSKIGKYLDNHIDILRFRSVCELFHSSIPPSLPNSPSFPLQIPRTIPVSLYNYLYQSTIYLIEPTNASSIFNLPPLAPSYTKGWLIKVEESKNNPLKSLLSPISDCKLSYPLDSNNSSPIIWNLLNYRVIELCKSYSFQKTPYSSSFVSKVVSYPNSPWINVGDCVACCIFQEGKLGFMKYGDTKWKLVDENNFYYDDVIVFKGQFYVTDKWGTISWINISSLKLIQFSPPLCGFGNKKHLVESCGNLYVVDRYYEGENMRGNNVGRRHDRDAVVECFKVYMLDEEWGKWVDVKNLRDRAFILSSSCNFSVSAKELIGYQGNCIYFKDTFNARMYSLDDNIIITVDFDPCIDKTLWSHSRWMRC*