AlfalfaGEDB | Msa1229320

M. sativa cultivar zhongmu-4 / Msa1229320

KEGG: K02921
GO: -
PFAM: PF01780.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1229320	XP_034932641.1	100.000	92	0	0	1	92	48	139	2.05e-59	188

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1229320	sp\|Q9ZRS8\|RL37A_PSEMZ	97.802	91	2	0	1	91	1	91	6.61e-60	180

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1229320	M5WNW1	98.913	92	1	0	1	92	37	128	8.10e-60	188

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027260	Msa1229320	0.830407	2.989154e-55	-8.615850e-47
Msa0049370	Msa1229320	0.806894	6.590865e-50	-8.615850e-47
Msa0608320	Msa1229320	0.802780	4.768184e-49	-8.615850e-47
Msa0621950	Msa1229320	0.837980	3.773858e-57	-8.615850e-47
Msa0784200	Msa1229320	0.854916	8.945208e-62	-8.615850e-47
Msa0792760	Msa1229320	0.800358	1.495993e-48	-8.615850e-47
Msa0818310	Msa1229320	0.849902	2.400079e-60	-8.615850e-47
Msa0122970	Msa1229320	0.808030	3.784370e-50	-8.615850e-47
Msa0128860	Msa1229320	0.804720	1.886331e-49	-8.615850e-47
Msa0140820	Msa1229320	0.825686	4.093422e-54	-8.615850e-47
Msa0140850	Msa1229320	0.820033	8.491047e-53	-8.615850e-47
Msa0174730	Msa1229320	0.802862	4.586142e-49	-8.615850e-47
Msa0189700	Msa1229320	0.814347	1.613476e-51	-8.615850e-47
Msa0229230	Msa1229320	0.826794	2.230389e-54	-8.615850e-47
Msa0229760	Msa1229320	0.807814	4.204885e-50	-8.615850e-47
Msa0232010	Msa1229320	0.819132	1.363662e-52	-8.615850e-47
Msa0232080	Msa1229320	0.821298	4.348949e-53	-8.615850e-47
Msa1229320	Msa1343260	0.825480	4.582431e-54	-8.615850e-47
Msa1229320	Msa1379760	0.800895	1.162826e-48	-8.615850e-47
Msa1229320	Msa1433310	0.807394	5.164971e-50	-8.615850e-47
Msa1229320	Msa1448690	0.801783	7.650414e-49	-8.615850e-47
Msa0369450	Msa1229320	0.872219	3.762666e-67	-8.615850e-47
Msa0427150	Msa1229320	0.800642	1.309730e-48	-8.615850e-47
Msa1013400	Msa1229320	0.822116	2.813186e-53	-8.615850e-47
Msa1019260	Msa1229320	0.810956	8.908311e-51	-8.615850e-47
Msa1093530	Msa1229320	0.820759	5.789530e-53	-8.615850e-47
Msa1104140	Msa1229320	0.810595	1.065908e-50	-8.615850e-47
Msa1104210	Msa1229320	0.804133	2.500330e-49	-8.615850e-47
Msa0280610	Msa1229320	0.836329	9.975972e-57	-8.615850e-47
Msa1126510	Msa1229320	0.837283	5.696330e-57	-8.615850e-47
Msa1165940	Msa1229320	0.830934	2.219763e-55	-8.615850e-47
Msa1166860	Msa1229320	0.834530	2.842732e-56	-8.615850e-47
Msa1183450	Msa1229320	0.876949	9.296864e-69	-8.615850e-47
Msa1218600	Msa1229320	0.820361	7.143205e-53	-8.615850e-47
Msa0518390	Msa1229320	0.816025	6.840588e-52	-8.615850e-47
Msa0518450	Msa1229320	0.812641	3.828320e-51	-8.615850e-47
Msa0524500	Msa1229320	0.806924	6.494575e-50	-8.615850e-47
Msa0588360	Msa1229320	0.815777	7.770519e-52	-8.615850e-47
Msa0858770	Msa1229320	0.860550	1.909786e-63	-8.615850e-47
Msa0881290	Msa1229320	0.814807	1.276495e-51	-8.615850e-47
Msa0902760	Msa1229320	0.819187	1.325020e-52	-8.615850e-47
Msa0953310	Msa1229320	0.823044	1.712096e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
Msa1229320	MtrunA17_Chr8g0336121	100.000	92	1	92	1	92	7.50e-63	185
Msa1229320	MtrunA17_Chr4g0055341	100.000	92	1	92	1	92	7.50e-63	185
Msa1229320	MtrunA17_Chr4g0057431	100.000	92	1	92	1	92	7.50e-63	185
Msa1229320	MtrunA17_Chr8g0336341	100.000	92	1	92	1	92	7.50e-63	185

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1229320	AT3G10950.1	93.478	92	6	0	1	92	1	92	4.50e-59	176
Msa1229320	AT3G60245.1	92.308	91	7	0	1	91	1	91	8.73e-58	172

Find 49 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCAACAATACCAGCCTTCT+TGG	0.219039	8_2:-5013065	None:intergenic
AGAGGAAGGCTGTCGGTATT+TGG	0.231684	8_2:+5014627	Msa1229320:CDS
TCCATTCAATCCACCGAAAA+TGG	0.247511	8_2:+5012910	Msa1229320:exon
GAGGAAGGCTGTCGGTATTT+GGG	0.275108	8_2:+5014628	Msa1229320:CDS
TATGGGTCTGTGACTTCTAA+TGG	0.282118	8_2:-5012798	None:intergenic
TAAATTTCAGGCACCCGATA+TGG	0.335327	8_2:+5014430	Msa1229320:intron
TCCTCAAACTAGCACCATAT+CGG	0.361347	8_2:-5014444	None:intergenic
CTATATGTTGTCTAGGGTTA+AGG	0.365835	8_2:+5015107	Msa1229320:three_prime_UTR
ATCAAATATAATCTTAAAAC+AGG	0.400478	8_2:-5014917	None:intergenic
GAGGAAGCAAATTAAGAAGA+TGG	0.413539	8_2:+5014462	Msa1229320:CDS
GCTGGTATTGTTGGCAAATA+CGG	0.422228	8_2:+5013073	Msa1229320:CDS
GCACGGTTACACTTCAACTA+AGG	0.425371	8_2:-5012865	None:intergenic
ACCAAGAAGGCTGGTATTGT+TGG	0.440905	8_2:+5013064	Msa1229320:CDS
CCCGATATGGTGCTAGTTTG+AGG	0.444329	8_2:+5014443	Msa1229320:CDS
CAGTCTGCTCCCTCAACCTT+CGG	0.446744	8_2:-5014830	None:intergenic
TATTTATAAGACTGCGAAAT+GGG	0.456126	8_2:-5012821	None:intergenic
TTATTTATAAGACTGCGAAA+TGG	0.457977	8_2:-5012822	None:intergenic
ATGGGTCTGTGACTTCTAAT+GGG	0.458034	8_2:-5012797	None:intergenic
TAATACAGTATGCTGTTAAG+AGG	0.458691	8_2:+5014609	Msa1229320:intron
TGATTTGCTATATGTTGTCT+AGG	0.463453	8_2:+5015100	Msa1229320:three_prime_UTR
GAACGACTTTCTTCTAATCC+TGG	0.467465	8_2:-5015186	None:intergenic
ATAAGACTGCGAAATGGGTA+TGG	0.475388	8_2:-5012816	None:intergenic
CCTCAAACTAGCACCATATC+GGG	0.503731	8_2:-5014443	None:intergenic
GAAGCACCATCCGAAGGTTG+AGG	0.512356	8_2:+5014820	Msa1229320:CDS
TTGAATGCATGCACAATACC+AGG	0.517484	8_2:+5015168	Msa1229320:three_prime_UTR
ATTTGGGGCTGCAAAGATTG+TGG	0.525352	8_2:+5014644	Msa1229320:CDS
GATTTGCTATATGTTGTCTA+GGG	0.527970	8_2:+5015101	Msa1229320:three_prime_UTR
AATGGAAGACGAGGGAATCA+AGG	0.533008	8_2:-5012893	None:intergenic
CACTTCAACTAAGGTTTGTG+AGG	0.533432	8_2:-5012856	None:intergenic
AAGCACCATCCGAAGGTTGA+GGG	0.541427	8_2:+5014821	Msa1229320:CDS
AGGAAGGCTGTCGGTATTTG+GGG	0.543829	8_2:+5014629	Msa1229320:CDS
CTGCTCCCTCAACCTTCGGA+TGG	0.565963	8_2:-5014826	None:intergenic
TAAGACTGCGAAATGGGTAT+GGG	0.568490	8_2:-5012815	None:intergenic
GATTGTGGAAAAGTGAAAGC+TGG	0.576075	8_2:+5014659	Msa1229320:CDS
ACAGACAAAGAGAACCAAGA+AGG	0.579258	8_2:+5013051	Msa1229320:intron
ACAAAGAGAACCAAGAAGGC+TGG	0.580496	8_2:+5013055	Msa1229320:CDS
AAATTCTTCTGCGAGTTCTG+TGG	0.586791	8_2:+5014502	Msa1229320:CDS
ACAGTATGCTGTTAAGAGGA+AGG	0.590694	8_2:+5014613	Msa1229320:intron
AGACGAGGGAATCAAGGTTG+AGG	0.600033	8_2:-5012887	None:intergenic
GCTTCTCACAGTTACAGCAC+TGG	0.604667	8_2:-5014802	None:intergenic
CTTCTGCGAGTTCTGTGGAA+AGG	0.605984	8_2:+5014507	Msa1229320:CDS
ACTGATTCATCATCGTAGGA+AGG	0.609651	8_2:-5015036	None:intergenic
CTGTGAGAAGCACCATCCGA+AGG	0.653930	8_2:+5014814	Msa1229320:CDS
GCTGTTAAGAGGAAGGCTGT+CGG	0.664645	8_2:+5014620	Msa1229320:CDS
TCAAATGCTATCTAGTTACG+TGG	0.670577	8_2:+5015009	Msa1229320:three_prime_UTR
GGTGGATTGAATGGAAGACG+AGG	0.673099	8_2:-5012902	None:intergenic
AGGGAATCAAGGTTGAGGCA+CGG	0.684740	8_2:-5012882	None:intergenic
GTGGATTGAATGGAAGACGA+GGG	0.693984	8_2:-5012901	None:intergenic
TGTGGAAAAGTGAAAGCTGG+CGG	0.773884	8_2:+5014662	Msa1229320:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATATTTAATTTCAACATTT+TGG	+	chr8_2:5013175-5013194	Msa1229320:intron	10.0%
!!!	ATATTTAATTTCAACATTTT+GGG	+	chr8_2:5013176-5013195	Msa1229320:intron	10.0%
!!!	TTTTAAGATTATATTTGATT+TGG	+	chr8_2:5014921-5014940	Msa1229320:three_prime_UTR	10.0%
!!!	AATATTTAATTTCAACATTT+TGG	+	chr8_2:5013175-5013194	Msa1229320:intron	10.0%
!!!	ATATTTAATTTCAACATTTT+GGG	+	chr8_2:5013176-5013195	Msa1229320:intron	10.0%
!!!	TTTTAAGATTATATTTGATT+TGG	+	chr8_2:5014921-5014940	Msa1229320:three_prime_UTR	10.0%
!!	AAAAATAAGCTAATCAAAAA+GGG	-	chr8_2:5013242-5013261	None:intergenic	15.0%
!!	AAAAAATAAGCTAATCAAAA+AGG	-	chr8_2:5013243-5013262	None:intergenic	15.0%
!!	ATTTAGATTGAATAAATTTC+AGG	+	chr8_2:5014418-5014437	Msa1229320:intron	15.0%
!!	ATCAAATATAATCTTAAAAC+AGG	-	chr8_2:5014920-5014939	None:intergenic	15.0%
!!!	GTTTTTTTTTTTTTGCTAAT+AGG	+	chr8_2:5015057-5015076	Msa1229320:three_prime_UTR	15.0%
!!	AAAAATAAGCTAATCAAAAA+GGG	-	chr8_2:5013242-5013261	None:intergenic	15.0%
!!	AAAAAATAAGCTAATCAAAA+AGG	-	chr8_2:5013243-5013262	None:intergenic	15.0%
!!	ATTTAGATTGAATAAATTTC+AGG	+	chr8_2:5014418-5014437	Msa1229320:intron	15.0%
!!	ATCAAATATAATCTTAAAAC+AGG	-	chr8_2:5014920-5014939	None:intergenic	15.0%
!!!	GTTTTTTTTTTTTTGCTAAT+AGG	+	chr8_2:5015057-5015076	Msa1229320:three_prime_UTR	15.0%
!!!	TATTGGTTTGTTCAATTTTA+AGG	+	chr8_2:5012961-5012980	Msa1229320:intron	20.0%
!!!	GCTTTTATGTATTATTCATA+AGG	-	chr8_2:5013364-5013383	None:intergenic	20.0%
!!	TTCTACATTCAAACTATTAA+GGG	-	chr8_2:5013432-5013451	None:intergenic	20.0%
!!	TTTCTACATTCAAACTATTA+AGG	-	chr8_2:5013433-5013452	None:intergenic	20.0%
!!	CTTCACTTACTAAAATTTAA+AGG	-	chr8_2:5013996-5014015	None:intergenic	20.0%
!!	AAATTGTCTTGAGTTAAAAT+TGG	-	chr8_2:5014303-5014322	None:intergenic	20.0%
!!!	AAGACAATTTTGTCAAATAT+TGG	+	chr8_2:5014313-5014332	Msa1229320:intron	20.0%
!!!	AGTGATTTATTTTTTGCTTT+TGG	+	chr8_2:5015131-5015150	Msa1229320:three_prime_UTR	20.0%
!!!	TTGCTTTTGGTAAATTATTT+TGG	+	chr8_2:5015144-5015163	Msa1229320:three_prime_UTR	20.0%
!!!	TGCTTTTGGTAAATTATTTT+GGG	+	chr8_2:5015145-5015164	Msa1229320:three_prime_UTR	20.0%
!!!	TATTGGTTTGTTCAATTTTA+AGG	+	chr8_2:5012961-5012980	Msa1229320:intron	20.0%
!!!	GCTTTTATGTATTATTCATA+AGG	-	chr8_2:5013364-5013383	None:intergenic	20.0%
!!	TTCTACATTCAAACTATTAA+GGG	-	chr8_2:5013432-5013451	None:intergenic	20.0%
!!	TTTCTACATTCAAACTATTA+AGG	-	chr8_2:5013433-5013452	None:intergenic	20.0%
!!	CTTCACTTACTAAAATTTAA+AGG	-	chr8_2:5013996-5014015	None:intergenic	20.0%
!!	AAATTGTCTTGAGTTAAAAT+TGG	-	chr8_2:5014303-5014322	None:intergenic	20.0%
!!!	AAGACAATTTTGTCAAATAT+TGG	+	chr8_2:5014313-5014332	Msa1229320:intron	20.0%
!!!	AGTGATTTATTTTTTGCTTT+TGG	+	chr8_2:5015131-5015150	Msa1229320:three_prime_UTR	20.0%
!!!	TTGCTTTTGGTAAATTATTT+TGG	+	chr8_2:5015144-5015163	Msa1229320:three_prime_UTR	20.0%
!!!	TGCTTTTGGTAAATTATTTT+GGG	+	chr8_2:5015145-5015164	Msa1229320:three_prime_UTR	20.0%
!	TATTTATAAGACTGCGAAAT+GGG	-	chr8_2:5012824-5012843	None:intergenic	25.0%
!	TTATTTATAAGACTGCGAAA+TGG	-	chr8_2:5012825-5012844	None:intergenic	25.0%
!!!	TGTTTTTTCATAAACTACCT+TGG	+	chr8_2:5013335-5013354	Msa1229320:intron	25.0%
!!!	TTATTTATTTTGCATCAGCT+CGG	+	chr8_2:5013384-5013403	Msa1229320:intron	25.0%
!	AAAACCCTTAAAATACTAGT+GGG	+	chr8_2:5013521-5013540	Msa1229320:intron	25.0%
!!	GTCACTTAAACTGATTATTT+TGG	+	chr8_2:5013928-5013947	Msa1229320:intron	25.0%
!	CACTTACTAAAATTTAAAGG+TGG	-	chr8_2:5013993-5014012	None:intergenic	25.0%
!!!	CAATTTTAACTTCAGAACAA+CGG	+	chr8_2:5014217-5014236	Msa1229320:intron	25.0%
!!	AATTATAACAGAGCTTCTTT+TGG	+	chr8_2:5014252-5014271	Msa1229320:intron	25.0%
!	TAGACAAGAAAATAAACCAA+GGG	-	chr8_2:5014731-5014750	None:intergenic	25.0%
!	ATAGACAAGAAAATAAACCA+AGG	-	chr8_2:5014732-5014751	None:intergenic	25.0%
!	CAAAACGACAAAAATGTAAA+TGG	-	chr8_2:5014867-5014886	None:intergenic	25.0%
!!!	CATTTTTGTCGTTTTGTTTT+TGG	+	chr8_2:5014871-5014890	Msa1229320:three_prime_UTR	25.0%
!	TATTTATAAGACTGCGAAAT+GGG	-	chr8_2:5012824-5012843	None:intergenic	25.0%
!	TTATTTATAAGACTGCGAAA+TGG	-	chr8_2:5012825-5012844	None:intergenic	25.0%
!!!	TGTTTTTTCATAAACTACCT+TGG	+	chr8_2:5013335-5013354	Msa1229320:intron	25.0%
!!!	TTATTTATTTTGCATCAGCT+CGG	+	chr8_2:5013384-5013403	Msa1229320:intron	25.0%
!	AAAACCCTTAAAATACTAGT+GGG	+	chr8_2:5013521-5013540	Msa1229320:intron	25.0%
!!	GTCACTTAAACTGATTATTT+TGG	+	chr8_2:5013928-5013947	Msa1229320:intron	25.0%
!	CACTTACTAAAATTTAAAGG+TGG	-	chr8_2:5013993-5014012	None:intergenic	25.0%
!!!	CAATTTTAACTTCAGAACAA+CGG	+	chr8_2:5014217-5014236	Msa1229320:intron	25.0%
!!	AATTATAACAGAGCTTCTTT+TGG	+	chr8_2:5014252-5014271	Msa1229320:intron	25.0%
!	TAGACAAGAAAATAAACCAA+GGG	-	chr8_2:5014731-5014750	None:intergenic	25.0%
!	ATAGACAAGAAAATAAACCA+AGG	-	chr8_2:5014732-5014751	None:intergenic	25.0%
!	CAAAACGACAAAAATGTAAA+TGG	-	chr8_2:5014867-5014886	None:intergenic	25.0%
!!!	CATTTTTGTCGTTTTGTTTT+TGG	+	chr8_2:5014871-5014890	Msa1229320:three_prime_UTR	25.0%
	TCTCTCTCTCTAATTGTTAT+TGG	+	chr8_2:5012944-5012963	Msa1229320:intron	30.0%
!	ACATGAATTTAGAACAACGT+TGG	-	chr8_2:5012998-5013017	None:intergenic	30.0%
!!!	TATGTTGCTTTTCAACATTC+TGG	+	chr8_2:5013096-5013115	Msa1229320:intron	30.0%
!!!	ATGTTGCTTTTCAACATTCT+GGG	+	chr8_2:5013097-5013116	Msa1229320:intron	30.0%
!!	TTTCAACATTTTGGGTGTTT+TGG	+	chr8_2:5013184-5013203	Msa1229320:intron	30.0%
!!!	CTCATCAATTTTAAGATCTG+AGG	+	chr8_2:5013216-5013235	Msa1229320:intron	30.0%
	TATTATTCATAAGGTTGCCA+AGG	-	chr8_2:5013355-5013374	None:intergenic	30.0%
	AAACTATTAAGGGCTTCATT+TGG	-	chr8_2:5013422-5013441	None:intergenic	30.0%
!	TTGAATGTAGAAAGCTTTGT+TGG	+	chr8_2:5013440-5013459	Msa1229320:intron	30.0%
	AACCCTAACGTTAAGATATA+AGG	+	chr8_2:5013491-5013510	Msa1229320:intron	30.0%
	ACCCTAACGTTAAGATATAA+GGG	+	chr8_2:5013492-5013511	Msa1229320:intron	30.0%
	CAAAACCCTTAAAATACTAG+TGG	+	chr8_2:5013520-5013539	Msa1229320:intron	30.0%
!!	CTAACCCACTAGTATTTTAA+GGG	-	chr8_2:5013528-5013547	None:intergenic	30.0%
	TTCGCTAAGTAAATGAACTA+GGG	+	chr8_2:5013588-5013607	Msa1229320:intron	30.0%
	AGAACAATGTTGTCAAATAG+TGG	+	chr8_2:5013785-5013804	Msa1229320:intron	30.0%
!	TTGTCAAATAGTGGCTATTT+TGG	+	chr8_2:5013794-5013813	Msa1229320:intron	30.0%
!!	CGTTGTTCTGAAGTTAAAAT+TGG	-	chr8_2:5014219-5014238	None:intergenic	30.0%
!	TTGTCAAATATTGGCTATAG+TGG	+	chr8_2:5014322-5014341	Msa1229320:intron	30.0%
!!	ATAGTGGATCTATAGTTTAG+CGG	+	chr8_2:5014338-5014357	Msa1229320:intron	30.0%
!	TGAATTCAATCTGCTTTGTT+TGG	+	chr8_2:5014553-5014572	Msa1229320:intron	30.0%
	TAATACAGTATGCTGTTAAG+AGG	+	chr8_2:5014609-5014628	Msa1229320:intron	30.0%
	AGACAAGAAAATAAACCAAG+GGG	-	chr8_2:5014730-5014749	None:intergenic	30.0%
!	AAAAACTGATTCATCATCGT+AGG	-	chr8_2:5015043-5015062	None:intergenic	30.0%
!!	TGATTTGCTATATGTTGTCT+AGG	+	chr8_2:5015100-5015119	Msa1229320:three_prime_UTR	30.0%
!!	GATTTGCTATATGTTGTCTA+GGG	+	chr8_2:5015101-5015120	Msa1229320:three_prime_UTR	30.0%
	TCTCTCTCTCTAATTGTTAT+TGG	+	chr8_2:5012944-5012963	Msa1229320:intron	30.0%
!	ACATGAATTTAGAACAACGT+TGG	-	chr8_2:5012998-5013017	None:intergenic	30.0%
!!!	TATGTTGCTTTTCAACATTC+TGG	+	chr8_2:5013096-5013115	Msa1229320:intron	30.0%
!!!	ATGTTGCTTTTCAACATTCT+GGG	+	chr8_2:5013097-5013116	Msa1229320:intron	30.0%
!!	TTTCAACATTTTGGGTGTTT+TGG	+	chr8_2:5013184-5013203	Msa1229320:intron	30.0%
!!!	CTCATCAATTTTAAGATCTG+AGG	+	chr8_2:5013216-5013235	Msa1229320:intron	30.0%
	TATTATTCATAAGGTTGCCA+AGG	-	chr8_2:5013355-5013374	None:intergenic	30.0%
	AAACTATTAAGGGCTTCATT+TGG	-	chr8_2:5013422-5013441	None:intergenic	30.0%
!	TTGAATGTAGAAAGCTTTGT+TGG	+	chr8_2:5013440-5013459	Msa1229320:intron	30.0%
	AACCCTAACGTTAAGATATA+AGG	+	chr8_2:5013491-5013510	Msa1229320:intron	30.0%
	ACCCTAACGTTAAGATATAA+GGG	+	chr8_2:5013492-5013511	Msa1229320:intron	30.0%
	CAAAACCCTTAAAATACTAG+TGG	+	chr8_2:5013520-5013539	Msa1229320:intron	30.0%
!!	CTAACCCACTAGTATTTTAA+GGG	-	chr8_2:5013528-5013547	None:intergenic	30.0%
	TTCGCTAAGTAAATGAACTA+GGG	+	chr8_2:5013588-5013607	Msa1229320:intron	30.0%
	AGAACAATGTTGTCAAATAG+TGG	+	chr8_2:5013785-5013804	Msa1229320:intron	30.0%
!	TTGTCAAATAGTGGCTATTT+TGG	+	chr8_2:5013794-5013813	Msa1229320:intron	30.0%
!!	CGTTGTTCTGAAGTTAAAAT+TGG	-	chr8_2:5014219-5014238	None:intergenic	30.0%
!	TTGTCAAATATTGGCTATAG+TGG	+	chr8_2:5014322-5014341	Msa1229320:intron	30.0%
!!	ATAGTGGATCTATAGTTTAG+CGG	+	chr8_2:5014338-5014357	Msa1229320:intron	30.0%
!	TGAATTCAATCTGCTTTGTT+TGG	+	chr8_2:5014553-5014572	Msa1229320:intron	30.0%
	TAATACAGTATGCTGTTAAG+AGG	+	chr8_2:5014609-5014628	Msa1229320:intron	30.0%
	AGACAAGAAAATAAACCAAG+GGG	-	chr8_2:5014730-5014749	None:intergenic	30.0%
!	AAAAACTGATTCATCATCGT+AGG	-	chr8_2:5015043-5015062	None:intergenic	30.0%
!!	TGATTTGCTATATGTTGTCT+AGG	+	chr8_2:5015100-5015119	Msa1229320:three_prime_UTR	30.0%
!!	GATTTGCTATATGTTGTCTA+GGG	+	chr8_2:5015101-5015120	Msa1229320:three_prime_UTR	30.0%
	ATTAAGGGCTTCATTTGGAT+TGG	-	chr8_2:5013417-5013436	None:intergenic	35.0%
	GCCCTTATATCTTAACGTTA+GGG	-	chr8_2:5013496-5013515	None:intergenic	35.0%
	TGCCCTTATATCTTAACGTT+AGG	-	chr8_2:5013497-5013516	None:intergenic	35.0%
!	CCTAACCCACTAGTATTTTA+AGG	-	chr8_2:5013529-5013548	None:intergenic	35.0%
!!	CCTTAAAATACTAGTGGGTT+AGG	+	chr8_2:5013526-5013545	Msa1229320:intron	35.0%
	GTTCGCTAAGTAAATGAACT+AGG	+	chr8_2:5013587-5013606	Msa1229320:intron	35.0%
!	TTGCGTCACTAGGATTTTAA+GGG	-	chr8_2:5013622-5013641	None:intergenic	35.0%
!	ATTGCGTCACTAGGATTTTA+AGG	-	chr8_2:5013623-5013642	None:intergenic	35.0%
	TAGATGTAAATTGCGTCACT+AGG	-	chr8_2:5013632-5013651	None:intergenic	35.0%
	TAACTTCAGAACAACGGTTA+TGG	+	chr8_2:5014223-5014242	Msa1229320:intron	35.0%
	AACTTCAGAACAACGGTTAT+GGG	+	chr8_2:5014224-5014243	Msa1229320:intron	35.0%
	GAGGAAGCAAATTAAGAAGA+TGG	+	chr8_2:5014462-5014481	Msa1229320:CDS	35.0%
	GACAAGAAAATAAACCAAGG+GGG	-	chr8_2:5014729-5014748	None:intergenic	35.0%
	AATACACAAGAACAGAAGCA+CGG	-	chr8_2:5014766-5014785	None:intergenic	35.0%
!	TGATGAACAAGAAGTTTTGC+TGG	+	chr8_2:5014949-5014968	Msa1229320:three_prime_UTR	35.0%
	TCAAATGCTATCTAGTTACG+TGG	+	chr8_2:5015009-5015028	Msa1229320:three_prime_UTR	35.0%
!	CTATATGTTGTCTAGGGTTA+AGG	+	chr8_2:5015107-5015126	Msa1229320:three_prime_UTR	35.0%
	ATTAAGGGCTTCATTTGGAT+TGG	-	chr8_2:5013417-5013436	None:intergenic	35.0%
	GCCCTTATATCTTAACGTTA+GGG	-	chr8_2:5013496-5013515	None:intergenic	35.0%
	TGCCCTTATATCTTAACGTT+AGG	-	chr8_2:5013497-5013516	None:intergenic	35.0%
!	CCTAACCCACTAGTATTTTA+AGG	-	chr8_2:5013529-5013548	None:intergenic	35.0%
!!	CCTTAAAATACTAGTGGGTT+AGG	+	chr8_2:5013526-5013545	Msa1229320:intron	35.0%
	GTTCGCTAAGTAAATGAACT+AGG	+	chr8_2:5013587-5013606	Msa1229320:intron	35.0%
!	TTGCGTCACTAGGATTTTAA+GGG	-	chr8_2:5013622-5013641	None:intergenic	35.0%
!	ATTGCGTCACTAGGATTTTA+AGG	-	chr8_2:5013623-5013642	None:intergenic	35.0%
	TAGATGTAAATTGCGTCACT+AGG	-	chr8_2:5013632-5013651	None:intergenic	35.0%
	TAACTTCAGAACAACGGTTA+TGG	+	chr8_2:5014223-5014242	Msa1229320:intron	35.0%
	AACTTCAGAACAACGGTTAT+GGG	+	chr8_2:5014224-5014243	Msa1229320:intron	35.0%
	GAGGAAGCAAATTAAGAAGA+TGG	+	chr8_2:5014462-5014481	Msa1229320:CDS	35.0%
	GACAAGAAAATAAACCAAGG+GGG	-	chr8_2:5014729-5014748	None:intergenic	35.0%
	AATACACAAGAACAGAAGCA+CGG	-	chr8_2:5014766-5014785	None:intergenic	35.0%
!	TGATGAACAAGAAGTTTTGC+TGG	+	chr8_2:5014949-5014968	Msa1229320:three_prime_UTR	35.0%
	TCAAATGCTATCTAGTTACG+TGG	+	chr8_2:5015009-5015028	Msa1229320:three_prime_UTR	35.0%
!	CTATATGTTGTCTAGGGTTA+AGG	+	chr8_2:5015107-5015126	Msa1229320:three_prime_UTR	35.0%
	ATGGGTCTGTGACTTCTAAT+GGG	-	chr8_2:5012800-5012819	None:intergenic	40.0%
	TATGGGTCTGTGACTTCTAA+TGG	-	chr8_2:5012801-5012820	None:intergenic	40.0%
	TAAGACTGCGAAATGGGTAT+GGG	-	chr8_2:5012818-5012837	None:intergenic	40.0%
	ATAAGACTGCGAAATGGGTA+TGG	-	chr8_2:5012819-5012838	None:intergenic	40.0%
	CACTTCAACTAAGGTTTGTG+AGG	-	chr8_2:5012859-5012878	None:intergenic	40.0%
	TCCATTCAATCCACCGAAAA+TGG	+	chr8_2:5012910-5012929	Msa1229320:exon	40.0%
!!!	ACCATTTTCGGTGGATTGAA+TGG	-	chr8_2:5012914-5012933	None:intergenic	40.0%
	ACAGACAAAGAGAACCAAGA+AGG	+	chr8_2:5013051-5013070	Msa1229320:intron	40.0%
	GCTGGTATTGTTGGCAAATA+CGG	+	chr8_2:5013073-5013092	Msa1229320:CDS	40.0%
	CTCAGCTAAGACGAGATTAT+GGG	+	chr8_2:5013666-5013685	Msa1229320:intron	40.0%
	TAAATTTCAGGCACCCGATA+TGG	+	chr8_2:5014430-5014449	Msa1229320:intron	40.0%
	TCCTCAAACTAGCACCATAT+CGG	-	chr8_2:5014447-5014466	None:intergenic	40.0%
	AAATTCTTCTGCGAGTTCTG+TGG	+	chr8_2:5014502-5014521	Msa1229320:CDS	40.0%
	GATTGAATTCATCGTCGTAG+AGG	-	chr8_2:5014544-5014563	None:intergenic	40.0%
	GTATTAACAACATCGCGTGA+CGG	-	chr8_2:5014595-5014614	None:intergenic	40.0%
	ACAGTATGCTGTTAAGAGGA+AGG	+	chr8_2:5014613-5014632	Msa1229320:intron	40.0%
	GATTGTGGAAAAGTGAAAGC+TGG	+	chr8_2:5014659-5014678	Msa1229320:CDS	40.0%
!	TTTGAATCTGTTTTCCCCCT+TGG	+	chr8_2:5014712-5014731	Msa1229320:intron	40.0%
!	ACTGATTCATCATCGTAGGA+AGG	-	chr8_2:5015039-5015058	None:intergenic	40.0%
	TTGAATGCATGCACAATACC+AGG	+	chr8_2:5015168-5015187	Msa1229320:three_prime_UTR	40.0%
	GAACGACTTTCTTCTAATCC+TGG	-	chr8_2:5015189-5015208	None:intergenic	40.0%
	ATGGGTCTGTGACTTCTAAT+GGG	-	chr8_2:5012800-5012819	None:intergenic	40.0%
	TATGGGTCTGTGACTTCTAA+TGG	-	chr8_2:5012801-5012820	None:intergenic	40.0%
	TAAGACTGCGAAATGGGTAT+GGG	-	chr8_2:5012818-5012837	None:intergenic	40.0%
	ATAAGACTGCGAAATGGGTA+TGG	-	chr8_2:5012819-5012838	None:intergenic	40.0%
	CACTTCAACTAAGGTTTGTG+AGG	-	chr8_2:5012859-5012878	None:intergenic	40.0%
	TCCATTCAATCCACCGAAAA+TGG	+	chr8_2:5012910-5012929	Msa1229320:exon	40.0%
!!!	ACCATTTTCGGTGGATTGAA+TGG	-	chr8_2:5012914-5012933	None:intergenic	40.0%
	ACAGACAAAGAGAACCAAGA+AGG	+	chr8_2:5013051-5013070	Msa1229320:intron	40.0%
	GCTGGTATTGTTGGCAAATA+CGG	+	chr8_2:5013073-5013092	Msa1229320:CDS	40.0%
	CTCAGCTAAGACGAGATTAT+GGG	+	chr8_2:5013666-5013685	Msa1229320:intron	40.0%
	TAAATTTCAGGCACCCGATA+TGG	+	chr8_2:5014430-5014449	Msa1229320:intron	40.0%
	TCCTCAAACTAGCACCATAT+CGG	-	chr8_2:5014447-5014466	None:intergenic	40.0%
	AAATTCTTCTGCGAGTTCTG+TGG	+	chr8_2:5014502-5014521	Msa1229320:CDS	40.0%
	GATTGAATTCATCGTCGTAG+AGG	-	chr8_2:5014544-5014563	None:intergenic	40.0%
	GTATTAACAACATCGCGTGA+CGG	-	chr8_2:5014595-5014614	None:intergenic	40.0%
	ACAGTATGCTGTTAAGAGGA+AGG	+	chr8_2:5014613-5014632	Msa1229320:intron	40.0%
	GATTGTGGAAAAGTGAAAGC+TGG	+	chr8_2:5014659-5014678	Msa1229320:CDS	40.0%
!	TTTGAATCTGTTTTCCCCCT+TGG	+	chr8_2:5014712-5014731	Msa1229320:intron	40.0%
!	ACTGATTCATCATCGTAGGA+AGG	-	chr8_2:5015039-5015058	None:intergenic	40.0%
	TTGAATGCATGCACAATACC+AGG	+	chr8_2:5015168-5015187	Msa1229320:three_prime_UTR	40.0%
	GAACGACTTTCTTCTAATCC+TGG	-	chr8_2:5015189-5015208	None:intergenic	40.0%
	GCACGGTTACACTTCAACTA+AGG	-	chr8_2:5012868-5012887	None:intergenic	45.0%
	AATGGAAGACGAGGGAATCA+AGG	-	chr8_2:5012896-5012915	None:intergenic	45.0%
!	GTGGATTGAATGGAAGACGA+GGG	-	chr8_2:5012904-5012923	None:intergenic	45.0%
!!	GAGAGAGCACGAACCATTTT+CGG	-	chr8_2:5012926-5012945	None:intergenic	45.0%
	ACAAAGAGAACCAAGAAGGC+TGG	+	chr8_2:5013055-5013074	Msa1229320:CDS	45.0%
	ACCAAGAAGGCTGGTATTGT+TGG	+	chr8_2:5013064-5013083	Msa1229320:CDS	45.0%
	CCATAATCTCGTCTTAGCTG+AGG	-	chr8_2:5013668-5013687	None:intergenic	45.0%
	CCTCAGCTAAGACGAGATTA+TGG	+	chr8_2:5013665-5013684	Msa1229320:intron	45.0%
	CCTCAAACTAGCACCATATC+GGG	-	chr8_2:5014446-5014465	None:intergenic	45.0%
!	ATTTGGGGCTGCAAAGATTG+TGG	+	chr8_2:5014644-5014663	Msa1229320:CDS	45.0%
	TGTGGAAAAGTGAAAGCTGG+CGG	+	chr8_2:5014662-5014681	Msa1229320:CDS	45.0%
	GCACGGTTACACTTCAACTA+AGG	-	chr8_2:5012868-5012887	None:intergenic	45.0%
	AATGGAAGACGAGGGAATCA+AGG	-	chr8_2:5012896-5012915	None:intergenic	45.0%
!	GTGGATTGAATGGAAGACGA+GGG	-	chr8_2:5012904-5012923	None:intergenic	45.0%
!!	GAGAGAGCACGAACCATTTT+CGG	-	chr8_2:5012926-5012945	None:intergenic	45.0%
	ACAAAGAGAACCAAGAAGGC+TGG	+	chr8_2:5013055-5013074	Msa1229320:CDS	45.0%
	ACCAAGAAGGCTGGTATTGT+TGG	+	chr8_2:5013064-5013083	Msa1229320:CDS	45.0%
	CCATAATCTCGTCTTAGCTG+AGG	-	chr8_2:5013668-5013687	None:intergenic	45.0%
	CCTCAGCTAAGACGAGATTA+TGG	+	chr8_2:5013665-5013684	Msa1229320:intron	45.0%
	CCTCAAACTAGCACCATATC+GGG	-	chr8_2:5014446-5014465	None:intergenic	45.0%
!	ATTTGGGGCTGCAAAGATTG+TGG	+	chr8_2:5014644-5014663	Msa1229320:CDS	45.0%
	TGTGGAAAAGTGAAAGCTGG+CGG	+	chr8_2:5014662-5014681	Msa1229320:CDS	45.0%
	AGGGAATCAAGGTTGAGGCA+CGG	-	chr8_2:5012885-5012904	None:intergenic	50.0%
	AGACGAGGGAATCAAGGTTG+AGG	-	chr8_2:5012890-5012909	None:intergenic	50.0%
!!	GGTGGATTGAATGGAAGACG+AGG	-	chr8_2:5012905-5012924	None:intergenic	50.0%
!	AGAGCACGAACCATTTTCGG+TGG	-	chr8_2:5012923-5012942	None:intergenic	50.0%
	GCCAACAATACCAGCCTTCT+TGG	-	chr8_2:5013068-5013087	None:intergenic	50.0%
!	CCCGATATGGTGCTAGTTTG+AGG	+	chr8_2:5014443-5014462	Msa1229320:CDS	50.0%
	CTTCTGCGAGTTCTGTGGAA+AGG	+	chr8_2:5014507-5014526	Msa1229320:CDS	50.0%
	GCTGTTAAGAGGAAGGCTGT+CGG	+	chr8_2:5014620-5014639	Msa1229320:CDS	50.0%
	AGAGGAAGGCTGTCGGTATT+TGG	+	chr8_2:5014627-5014646	Msa1229320:CDS	50.0%
	GAGGAAGGCTGTCGGTATTT+GGG	+	chr8_2:5014628-5014647	Msa1229320:CDS	50.0%
	AGGAAGGCTGTCGGTATTTG+GGG	+	chr8_2:5014629-5014648	Msa1229320:CDS	50.0%
	GCTTCTCACAGTTACAGCAC+TGG	-	chr8_2:5014805-5014824	None:intergenic	50.0%
	AAGCACCATCCGAAGGTTGA+GGG	+	chr8_2:5014821-5014840	Msa1229320:CDS	50.0%
	AGGGAATCAAGGTTGAGGCA+CGG	-	chr8_2:5012885-5012904	None:intergenic	50.0%
	AGACGAGGGAATCAAGGTTG+AGG	-	chr8_2:5012890-5012909	None:intergenic	50.0%
!!	GGTGGATTGAATGGAAGACG+AGG	-	chr8_2:5012905-5012924	None:intergenic	50.0%
!	AGAGCACGAACCATTTTCGG+TGG	-	chr8_2:5012923-5012942	None:intergenic	50.0%
	GCCAACAATACCAGCCTTCT+TGG	-	chr8_2:5013068-5013087	None:intergenic	50.0%
!	CCCGATATGGTGCTAGTTTG+AGG	+	chr8_2:5014443-5014462	Msa1229320:CDS	50.0%
	CTTCTGCGAGTTCTGTGGAA+AGG	+	chr8_2:5014507-5014526	Msa1229320:CDS	50.0%
	GCTGTTAAGAGGAAGGCTGT+CGG	+	chr8_2:5014620-5014639	Msa1229320:CDS	50.0%
	AGAGGAAGGCTGTCGGTATT+TGG	+	chr8_2:5014627-5014646	Msa1229320:CDS	50.0%
	GAGGAAGGCTGTCGGTATTT+GGG	+	chr8_2:5014628-5014647	Msa1229320:CDS	50.0%
	AGGAAGGCTGTCGGTATTTG+GGG	+	chr8_2:5014629-5014648	Msa1229320:CDS	50.0%
	GCTTCTCACAGTTACAGCAC+TGG	-	chr8_2:5014805-5014824	None:intergenic	50.0%
	AAGCACCATCCGAAGGTTGA+GGG	+	chr8_2:5014821-5014840	Msa1229320:CDS	50.0%
!!	CTGTGAGAAGCACCATCCGA+AGG	+	chr8_2:5014814-5014833	Msa1229320:CDS	55.0%
!	GAAGCACCATCCGAAGGTTG+AGG	+	chr8_2:5014820-5014839	Msa1229320:CDS	55.0%
	CAGTCTGCTCCCTCAACCTT+CGG	-	chr8_2:5014833-5014852	None:intergenic	55.0%
!!	CTGTGAGAAGCACCATCCGA+AGG	+	chr8_2:5014814-5014833	Msa1229320:CDS	55.0%
!	GAAGCACCATCCGAAGGTTG+AGG	+	chr8_2:5014820-5014839	Msa1229320:CDS	55.0%
	CAGTCTGCTCCCTCAACCTT+CGG	-	chr8_2:5014833-5014852	None:intergenic	55.0%
	CTGCTCCCTCAACCTTCGGA+TGG	-	chr8_2:5014829-5014848	None:intergenic	60.0%
	CTGCTCCCTCAACCTTCGGA+TGG	-	chr8_2:5014829-5014848	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	5012796	5015210	5012796	ID=Msa1229320;Name=Msa1229320
chr8_2	mRNA	5012796	5015210	5012796	ID=Msa1229320-mRNA-1;Parent=Msa1229320;Name=Msa1229320-mRNA-1;_AED=0.02;_eAED=0.02;_QI=133\|1\|1\|1\|1\|1\|5\|350\|92
chr8_2	exon	5012796	5012931	5012796	ID=Msa1229320-mRNA-1:exon:1702;Parent=Msa1229320-mRNA-1
chr8_2	exon	5013055	5013094	5013055	ID=Msa1229320-mRNA-1:exon:1703;Parent=Msa1229320-mRNA-1
chr8_2	exon	5014440	5014528	5014440	ID=Msa1229320-mRNA-1:exon:1704;Parent=Msa1229320-mRNA-1
chr8_2	exon	5014617	5014699	5014617	ID=Msa1229320-mRNA-1:exon:1705;Parent=Msa1229320-mRNA-1
chr8_2	exon	5014797	5015210	5014797	ID=Msa1229320-mRNA-1:exon:1706;Parent=Msa1229320-mRNA-1
chr8_2	five_prime_UTR	5012796	5012928	5012796	ID=Msa1229320-mRNA-1:five_prime_utr;Parent=Msa1229320-mRNA-1
chr8_2	CDS	5012929	5012931	5012929	ID=Msa1229320-mRNA-1:cds;Parent=Msa1229320-mRNA-1
chr8_2	CDS	5013055	5013094	5013055	ID=Msa1229320-mRNA-1:cds;Parent=Msa1229320-mRNA-1
chr8_2	CDS	5014440	5014528	5014440	ID=Msa1229320-mRNA-1:cds;Parent=Msa1229320-mRNA-1
chr8_2	CDS	5014617	5014699	5014617	ID=Msa1229320-mRNA-1:cds;Parent=Msa1229320-mRNA-1
chr8_2	CDS	5014797	5014860	5014797	ID=Msa1229320-mRNA-1:cds;Parent=Msa1229320-mRNA-1
chr8_2	three_prime_UTR	5014861	5015210	5014861	ID=Msa1229320-mRNA-1:three_prime_utr;Parent=Msa1229320-mRNA-1

Gene Sequence	>Msa1229320 ATGACAAAGAGAACCAAGAAGGCTGGTATTGTTGGCAAATACGGCACCCGATATGGTGCTAGTTTGAGGAAGCAAATTAAGAAGATGGAAGTCAGTCAGCACAGTAAATTCTTCTGCGAGTTCTGTGGAAAGTATGCTGTTAAGAGGAAGGCTGTCGGTATTTGGGGCTGCAAAGATTGTGGAAAAGTGAAAGCTGGCGGTGCTTACACTTTGAATACTGCCAGTGCTGTAACTGTGAGAAGCACCATCCGAAGGTTGAGGGAGCAGACTGAGAGTTAG
Protein sequence	>Msa1229320 MTKRTKKAGIVGKYGTRYGASLRKQIKKMEVSQHSKFFCEFCGKYAVKRKAVGIWGCKDCGKVKAGGAYTLNTASAVTVRSTIRRLREQTES*