AlfalfaGEDB | Msa1293400

M. sativa cultivar zhongmu-4 / Msa1293400

KEGG: -
GO: -
PFAM: PF14368.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1293400	XP_013446213.1	95.000	120	6	0	1	120	1	120	2.88e-74	226

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1293400	A0A072U339	95.000	120	6	0	1	120	1	120	1.37e-74	226

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0016540	Msa1293400	0.821483	3.941931e-53	-8.615850e-47
Msa0041690	Msa1293400	0.826809	2.213197e-54	-8.615850e-47
Msa0116790	Msa1293400	0.815983	6.988866e-52	-8.615850e-47
Msa0621840	Msa1293400	0.807460	4.999661e-50	-8.615850e-47
Msa0628420	Msa1293400	0.801732	7.837122e-49	-8.615850e-47
Msa0628500	Msa1293400	0.815867	7.420229e-52	-8.615850e-47
Msa0713340	Msa1293400	0.826672	2.385925e-54	-8.615850e-47
Msa0754930	Msa1293400	0.824940	6.148245e-54	-8.615850e-47
Msa0789560	Msa1293400	0.821961	3.056822e-53	-8.615850e-47
Msa0799340	Msa1293400	0.826316	2.899754e-54	-8.615850e-47
Msa0834330	Msa1293400	0.823467	1.363889e-53	-8.615850e-47
Msa0136120	Msa1293400	0.808041	3.763284e-50	-8.615850e-47
Msa0223330	Msa1293400	0.805851	1.093107e-49	-8.615850e-47
Msa1252610	Msa1293400	0.944859	8.964919e-104	-8.615850e-47
Msa1258980	Msa1293400	0.800628	1.318081e-48	-8.615850e-47
Msa1259640	Msa1293400	0.818433	1.965401e-52	-8.615850e-47
Msa1266020	Msa1293400	0.809953	1.466304e-50	-8.615850e-47
Msa1293390	Msa1293400	0.829881	4.017039e-55	-8.615850e-47
Msa1293400	Msa1320820	0.802194	6.298692e-49	-8.615850e-47
Msa1293400	Msa1329790	0.811358	7.287734e-51	-8.615850e-47
Msa1293400	Msa1337170	0.800188	1.620220e-48	-8.615850e-47
Msa1293400	Msa1344310	0.824693	7.028063e-54	-8.615850e-47
Msa1293400	Msa1356610	0.822063	2.894691e-53	-8.615850e-47
Msa1293400	Msa1362420	0.805816	1.111930e-49	-8.615850e-47
Msa1293400	Msa1410390	0.819158	1.344793e-52	-8.615850e-47
Msa1293400	Msa1411000	0.810893	9.191725e-51	-8.615850e-47
Msa1293400	Msa1411950	0.811569	6.555490e-51	-8.615850e-47
Msa1293400	Msa1416260	0.801992	6.929688e-49	-8.615850e-47
Msa1293400	Msa1432480	0.800224	1.593623e-48	-8.615850e-47
Msa0395980	Msa1293400	0.825445	4.669576e-54	-8.615850e-47
Msa0416960	Msa1293400	0.826023	3.405790e-54	-8.615850e-47
Msa0432070	Msa1293400	0.809090	2.246798e-50	-8.615850e-47
Msa0440510	Msa1293400	0.809245	2.081376e-50	-8.615850e-47
Msa0482040	Msa1293400	0.819243	1.286420e-52	-8.615850e-47
Msa1010460	Msa1293400	0.809995	1.436325e-50	-8.615850e-47
Msa1027360	Msa1293400	0.807082	6.013488e-50	-8.615850e-47
Msa1072600	Msa1293400	0.820605	6.278295e-53	-8.615850e-47
Msa0244970	Msa1293400	0.820637	6.174553e-53	-8.615850e-47
Msa0244980	Msa1293400	0.813802	2.128044e-51	-8.615850e-47
Msa0249310	Msa1293400	0.818959	1.492934e-52	-8.615850e-47
Msa0283420	Msa1293400	0.833106	6.453616e-56	-8.615850e-47
Msa0287790	Msa1293400	0.804111	2.526689e-49	-8.615850e-47
Msa0311720	Msa1293400	0.800040	1.736488e-48	-8.615850e-47
Msa0321690	Msa1293400	0.809355	1.971081e-50	-8.615850e-47
Msa0322900	Msa1293400	0.809806	1.577368e-50	-8.615850e-47
Msa0363060	Msa1293400	0.856202	3.773713e-62	-8.615850e-47
Msa1205480	Msa1293400	0.805004	1.645595e-49	-8.615850e-47
Msa1205590	Msa1293400	0.810767	9.787399e-51	-8.615850e-47
Msa1208790	Msa1293400	0.810259	1.259964e-50	-8.615850e-47
Msa1209670	Msa1293400	0.824574	7.499559e-54	-8.615850e-47
Msa1211340	Msa1293400	0.817189	3.751574e-52	-8.615850e-47
Msa0484740	Msa1293400	0.807121	5.900062e-50	-8.615850e-47
Msa0491000	Msa1293400	0.810181	1.309803e-50	-8.615850e-47
Msa0531250	Msa1293400	0.803941	2.741116e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1293400	MtrunA17_Chr8g0368861	95.000	120	6	0	1	120	1	120	2.65e-78	226
Msa1293400	MtrunA17_Chr8g0368831	94.167	120	7	0	1	120	9	128	1.22e-77	224
Msa1293400	MtrunA17_Chr7g0240471	51.515	99	46	2	23	120	20	117	1.23e-26	95.5
Msa1293400	MtrunA17_Chr7g0240451	51.579	95	44	2	27	120	24	117	5.45e-25	91.3
Msa1293400	MtrunA17_Chr7g0240441	57.407	54	22	1	63	116	38	90	2.17e-14	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1293400	AT2G37870.1	67.797	118	35	1	3	120	1	115	1.70e-50	156
Msa1293400	AT5G05960.1	45.902	122	58	3	1	120	1	116	2.69e-29	102
Msa1293400	AT3G53980.2	46.809	94	49	1	27	120	22	114	2.10e-27	97.8
Msa1293400	AT3G53980.1	46.809	94	49	1	27	120	22	114	2.10e-27	97.8

Find 33 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCATCAAGTGTGTTAATTT+TGG	0.223634	8_3:-50503241	Msa1293400:CDS
TTAATTCAATTAAATTATAA+TGG	0.325426	8_3:+50502144	None:intergenic
GTGAGACTATATTTGTCATT+TGG	0.337993	8_3:+50502017	None:intergenic
AGCTAGTCTAAGTCCATGTT+TGG	0.350440	8_3:-50503169	Msa1293400:CDS
AATTAAATTATAATGGAAGT+TGG	0.376374	8_3:+50502151	None:intergenic
TTCCTAATGTTGCATCTCTT+AGG	0.378053	8_3:+50502994	None:intergenic
GTGACTAACTAGTTTGTATA+TGG	0.382759	8_3:+50503357	None:intergenic
TGGGTGCTGTACGTAATGTT+AGG	0.392658	8_3:-50503149	Msa1293400:CDS
GAATGTGGAAGGACACCAAT+TGG	0.434211	8_3:-50503201	Msa1293400:CDS
AACAATAGTGATGGAGTTCA+AGG	0.437224	8_3:-50503301	Msa1293400:exon
AGAACAGAACAAAGACATCT+TGG	0.469439	8_3:+50503072	None:intergenic
CTTCCACATTTCTTTCCAGC+AGG	0.469545	8_3:+50502967	None:intergenic
CCAACTCTAGCACAACAAAC+TGG	0.478057	8_3:+50503114	None:intergenic
CTAGCTGCTGCTGACCCAAT+TGG	0.492373	8_3:+50503186	None:intergenic
TTCCTAAGAGATGCAACATT+AGG	0.493031	8_3:-50502996	Msa1293400:CDS
GGGTGCTGTACGTAATGTTA+GGG	0.506383	8_3:-50503148	Msa1293400:CDS
CCAGTTTGTTGTGCTAGAGT+TGG	0.508251	8_3:-50503114	Msa1293400:CDS
CACATTTCTTTCCAGCAGGT+CGG	0.514268	8_3:+50502971	None:intergenic
GCTGAGGCAGCTGGAGAATG+TGG	0.520830	8_3:-50503216	Msa1293400:CDS
AATGTGGAAGGACACCAATT+GGG	0.523864	8_3:-50503200	Msa1293400:CDS
TCATAAATACAAAATGCTAC+AGG	0.524540	8_3:+50502239	None:intergenic
AACATTAGGAACCGACCTGC+TGG	0.527493	8_3:-50502982	Msa1293400:CDS
GCTAGTCTAAGTCCATGTTT+GGG	0.544347	8_3:-50503168	Msa1293400:CDS
AGTTGGAGAAAATAACAGTA+AGG	0.551063	8_3:+50502168	None:intergenic
GGAACAGTGATAGCAATAGC+AGG	0.582273	8_3:+50503015	None:intergenic
TAGAACTTTCCTAGCCATAG+TGG	0.583702	8_3:-50503277	Msa1293400:CDS
GCTAAATAAATTCTGTAACA+TGG	0.587936	8_3:-50502293	Msa1293400:three_prime_UTR
AAAACATCACCACTATGGCT+AGG	0.592042	8_3:+50503268	None:intergenic
TTTATGAGTGTATTATTGCA+TGG	0.595193	8_3:-50502223	Msa1293400:three_prime_UTR
TTACGTACAGCACCCAAACA+TGG	0.602239	8_3:+50503156	None:intergenic
AGGCAGCTGGAGAATGTGGA+AGG	0.609221	8_3:-50503212	Msa1293400:CDS
CGACCTGCTGGAAAGAAATG+TGG	0.609362	8_3:-50502970	Msa1293400:CDS
ACTCTAGCACAACAAACTGG+TGG	0.633479	8_3:+50503117	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAAATATGAATCAATGAT+AGG	+	chr8_3:50502754-50502773	None:intergenic	15.0%
!!!	AATTAAATTATAATGGAAGT+TGG	+	chr8_3:50503240-50503259	None:intergenic	15.0%
!!	TAAAAATATGAATCAATGAT+AGG	+	chr8_3:50502754-50502773	None:intergenic	15.0%
!!!	AATTAAATTATAATGGAAGT+TGG	+	chr8_3:50503240-50503259	None:intergenic	15.0%
!!	AAAATACAAGGTTAAACTAA+AGG	-	chr8_3:50502674-50502693	Msa1293400:intron	20.0%
!!	AGAATTTGTAAAACTAAAAC+AGG	-	chr8_3:50502788-50502807	Msa1293400:intron	20.0%
!!!	TGTCATTTAAACTTTGTTTT+TGG	+	chr8_3:50502827-50502846	None:intergenic	20.0%
!!	AAAATACAAGGTTAAACTAA+AGG	-	chr8_3:50502674-50502693	Msa1293400:intron	20.0%
!!	AGAATTTGTAAAACTAAAAC+AGG	-	chr8_3:50502788-50502807	Msa1293400:intron	20.0%
!!!	TGTCATTTAAACTTTGTTTT+TGG	+	chr8_3:50502827-50502846	None:intergenic	20.0%
!	ATTCTCAAAAACAATAGTGA+TGG	-	chr8_3:50502078-50502097	Msa1293400:three_prime_UTR	25.0%
!	GTTCGAAATAGTAAAATACA+AGG	-	chr8_3:50502662-50502681	Msa1293400:intron	25.0%
!	TATGAATCAATGATAGGTTA+AGG	+	chr8_3:50502748-50502767	None:intergenic	25.0%
!!!	ACCTTCTTTTTTTGTTTTAC+AGG	-	chr8_3:50503003-50503022	Msa1293400:CDS	25.0%
!!!	CCTTCTTTTTTTGTTTTACA+GGG	-	chr8_3:50503004-50503023	Msa1293400:CDS	25.0%
!!	CGTATATTATTTTCTTGCTT+TGG	+	chr8_3:50503047-50503066	None:intergenic	25.0%
!	GCTAAATAAATTCTGTAACA+TGG	-	chr8_3:50503095-50503114	Msa1293400:CDS	25.0%
!	TCATAAATACAAAATGCTAC+AGG	+	chr8_3:50503152-50503171	None:intergenic	25.0%
!	TTTATGAGTGTATTATTGCA+TGG	-	chr8_3:50503165-50503184	Msa1293400:CDS	25.0%
!	TGTACGACTAAAAAATTTGT+CGG	-	chr8_3:50503301-50503320	Msa1293400:exon	25.0%
!	ATTCTCAAAAACAATAGTGA+TGG	-	chr8_3:50502078-50502097	Msa1293400:three_prime_UTR	25.0%
!	GTTCGAAATAGTAAAATACA+AGG	-	chr8_3:50502662-50502681	Msa1293400:intron	25.0%
!	TATGAATCAATGATAGGTTA+AGG	+	chr8_3:50502748-50502767	None:intergenic	25.0%
!!!	ACCTTCTTTTTTTGTTTTAC+AGG	-	chr8_3:50503003-50503022	Msa1293400:CDS	25.0%
!!!	CCTTCTTTTTTTGTTTTACA+GGG	-	chr8_3:50503004-50503023	Msa1293400:CDS	25.0%
!!	CGTATATTATTTTCTTGCTT+TGG	+	chr8_3:50503047-50503066	None:intergenic	25.0%
!	GCTAAATAAATTCTGTAACA+TGG	-	chr8_3:50503095-50503114	Msa1293400:CDS	25.0%
!	TCATAAATACAAAATGCTAC+AGG	+	chr8_3:50503152-50503171	None:intergenic	25.0%
!	TTTATGAGTGTATTATTGCA+TGG	-	chr8_3:50503165-50503184	Msa1293400:CDS	25.0%
!	TGTACGACTAAAAAATTTGT+CGG	-	chr8_3:50503301-50503320	Msa1293400:exon	25.0%
!	GTGACTAACTAGTTTGTATA+TGG	+	chr8_3:50502034-50502053	None:intergenic	30.0%
	CAAAAAAAACATCACCACTA+TGG	+	chr8_3:50502128-50502147	None:intergenic	30.0%
!!!	CATAGTGGTGATGTTTTTTT+TGG	-	chr8_3:50502126-50502145	Msa1293400:three_prime_UTR	30.0%
	TTCATATTGTAGGCACAATA+AGG	+	chr8_3:50502543-50502562	None:intergenic	30.0%
	AACTAGTAGCTTCATATTGT+AGG	+	chr8_3:50502553-50502572	None:intergenic	30.0%
!!	TTAAGGTGTTTTCAGAACTA+AGG	+	chr8_3:50502731-50502750	None:intergenic	30.0%
	TATGTTCTATTTGTGTCAAG+TGG	+	chr8_3:50502868-50502887	None:intergenic	30.0%
	CCCTGTAAAACAAAAAAAGA+AGG	+	chr8_3:50503007-50503026	None:intergenic	30.0%
!!	ATTTTCTTGCTTTGGTTTCA+AGG	+	chr8_3:50503039-50503058	None:intergenic	30.0%
	AGTTGGAGAAAATAACAGTA+AGG	+	chr8_3:50503223-50503242	None:intergenic	30.0%
!	GTGACTAACTAGTTTGTATA+TGG	+	chr8_3:50502034-50502053	None:intergenic	30.0%
	CAAAAAAAACATCACCACTA+TGG	+	chr8_3:50502128-50502147	None:intergenic	30.0%
!!!	CATAGTGGTGATGTTTTTTT+TGG	-	chr8_3:50502126-50502145	Msa1293400:three_prime_UTR	30.0%
	TTCATATTGTAGGCACAATA+AGG	+	chr8_3:50502543-50502562	None:intergenic	30.0%
	AACTAGTAGCTTCATATTGT+AGG	+	chr8_3:50502553-50502572	None:intergenic	30.0%
!!	TTAAGGTGTTTTCAGAACTA+AGG	+	chr8_3:50502731-50502750	None:intergenic	30.0%
	TATGTTCTATTTGTGTCAAG+TGG	+	chr8_3:50502868-50502887	None:intergenic	30.0%
	CCCTGTAAAACAAAAAAAGA+AGG	+	chr8_3:50503007-50503026	None:intergenic	30.0%
!!	ATTTTCTTGCTTTGGTTTCA+AGG	+	chr8_3:50503039-50503058	None:intergenic	30.0%
	AGTTGGAGAAAATAACAGTA+AGG	+	chr8_3:50503223-50503242	None:intergenic	30.0%
!	AACAATAGTGATGGAGTTCA+AGG	-	chr8_3:50502087-50502106	Msa1293400:three_prime_UTR	35.0%
!	GGCATCAAGTGTGTTAATTT+TGG	-	chr8_3:50502147-50502166	Msa1293400:three_prime_UTR	35.0%
!!	TGTGTTAATTTTGGAAGCTG+AGG	-	chr8_3:50502156-50502175	Msa1293400:three_prime_UTR	35.0%
	AGAACAGAACAAAGACATCT+TGG	+	chr8_3:50502319-50502338	None:intergenic	35.0%
!	TTCTGTTCTTTTGTCACCTA+TGG	-	chr8_3:50502330-50502349	Msa1293400:three_prime_UTR	35.0%
!!!	ATCTTAGCTTGTTTTGCCAT+AGG	+	chr8_3:50502349-50502368	None:intergenic	35.0%
	TTCCTAAGAGATGCAACATT+AGG	-	chr8_3:50502392-50502411	Msa1293400:intron	35.0%
	TTCCTAATGTTGCATCTCTT+AGG	+	chr8_3:50502397-50502416	None:intergenic	35.0%
	TTATCATTTCTCACATCCCA+AGG	+	chr8_3:50502596-50502615	None:intergenic	35.0%
	AAATGTACCAAGTGTCTGTT+TGG	+	chr8_3:50502632-50502651	None:intergenic	35.0%
!	TGTGCACATTTTGCAAAACT+TGG	-	chr8_3:50502888-50502907	Msa1293400:intron	35.0%
!	GTAACATGGCATTTTCTACT+TGG	-	chr8_3:50503109-50503128	Msa1293400:CDS	35.0%
!	AACAATAGTGATGGAGTTCA+AGG	-	chr8_3:50502087-50502106	Msa1293400:three_prime_UTR	35.0%
!	GGCATCAAGTGTGTTAATTT+TGG	-	chr8_3:50502147-50502166	Msa1293400:three_prime_UTR	35.0%
!!	TGTGTTAATTTTGGAAGCTG+AGG	-	chr8_3:50502156-50502175	Msa1293400:three_prime_UTR	35.0%
	AGAACAGAACAAAGACATCT+TGG	+	chr8_3:50502319-50502338	None:intergenic	35.0%
!	TTCTGTTCTTTTGTCACCTA+TGG	-	chr8_3:50502330-50502349	Msa1293400:three_prime_UTR	35.0%
!!!	ATCTTAGCTTGTTTTGCCAT+AGG	+	chr8_3:50502349-50502368	None:intergenic	35.0%
	TTCCTAAGAGATGCAACATT+AGG	-	chr8_3:50502392-50502411	Msa1293400:intron	35.0%
	TTCCTAATGTTGCATCTCTT+AGG	+	chr8_3:50502397-50502416	None:intergenic	35.0%
	TTATCATTTCTCACATCCCA+AGG	+	chr8_3:50502596-50502615	None:intergenic	35.0%
	AAATGTACCAAGTGTCTGTT+TGG	+	chr8_3:50502632-50502651	None:intergenic	35.0%
!	TGTGCACATTTTGCAAAACT+TGG	-	chr8_3:50502888-50502907	Msa1293400:intron	35.0%
!	GTAACATGGCATTTTCTACT+TGG	-	chr8_3:50503109-50503128	Msa1293400:CDS	35.0%
	TAGAACTTTCCTAGCCATAG+TGG	-	chr8_3:50502111-50502130	Msa1293400:three_prime_UTR	40.0%
	AAAACATCACCACTATGGCT+AGG	+	chr8_3:50502123-50502142	None:intergenic	40.0%
	AATGTGGAAGGACACCAATT+GGG	-	chr8_3:50502188-50502207	Msa1293400:three_prime_UTR	40.0%
!	AGCTAGTCTAAGTCCATGTT+TGG	-	chr8_3:50502219-50502238	Msa1293400:three_prime_UTR	40.0%
!	GCTAGTCTAAGTCCATGTTT+GGG	-	chr8_3:50502220-50502239	Msa1293400:three_prime_UTR	40.0%
	TAGGCACAATAAGGAATGTG+CGG	+	chr8_3:50502534-50502553	None:intergenic	40.0%
	TACAGTTCCAAACAGACACT+TGG	-	chr8_3:50502622-50502641	Msa1293400:intron	40.0%
!	AGGTGTTTTCAGAACTAAGG+TGG	+	chr8_3:50502728-50502747	None:intergenic	40.0%
	TAGAACTTTCCTAGCCATAG+TGG	-	chr8_3:50502111-50502130	Msa1293400:three_prime_UTR	40.0%
	AAAACATCACCACTATGGCT+AGG	+	chr8_3:50502123-50502142	None:intergenic	40.0%
	AATGTGGAAGGACACCAATT+GGG	-	chr8_3:50502188-50502207	Msa1293400:three_prime_UTR	40.0%
!	AGCTAGTCTAAGTCCATGTT+TGG	-	chr8_3:50502219-50502238	Msa1293400:three_prime_UTR	40.0%
!	GCTAGTCTAAGTCCATGTTT+GGG	-	chr8_3:50502220-50502239	Msa1293400:three_prime_UTR	40.0%
	TAGGCACAATAAGGAATGTG+CGG	+	chr8_3:50502534-50502553	None:intergenic	40.0%
	TACAGTTCCAAACAGACACT+TGG	-	chr8_3:50502622-50502641	Msa1293400:intron	40.0%
!	AGGTGTTTTCAGAACTAAGG+TGG	+	chr8_3:50502728-50502747	None:intergenic	40.0%
	GAATGTGGAAGGACACCAAT+TGG	-	chr8_3:50502187-50502206	Msa1293400:three_prime_UTR	45.0%
	TTACGTACAGCACCCAAACA+TGG	+	chr8_3:50502235-50502254	None:intergenic	45.0%
!	TGGGTGCTGTACGTAATGTT+AGG	-	chr8_3:50502239-50502258	Msa1293400:three_prime_UTR	45.0%
!	GGGTGCTGTACGTAATGTTA+GGG	-	chr8_3:50502240-50502259	Msa1293400:three_prime_UTR	45.0%
	ACTCTAGCACAACAAACTGG+TGG	+	chr8_3:50502274-50502293	None:intergenic	45.0%
	CCAACTCTAGCACAACAAAC+TGG	+	chr8_3:50502277-50502296	None:intergenic	45.0%
!!	CCAGTTTGTTGTGCTAGAGT+TGG	-	chr8_3:50502274-50502293	Msa1293400:three_prime_UTR	45.0%
	GGAACAGTGATAGCAATAGC+AGG	+	chr8_3:50502376-50502395	None:intergenic	45.0%
	CACATTTCTTTCCAGCAGGT+CGG	+	chr8_3:50502420-50502439	None:intergenic	45.0%
	CTTCCACATTTCTTTCCAGC+AGG	+	chr8_3:50502424-50502443	None:intergenic	45.0%
	GAATGTGGAAGGACACCAAT+TGG	-	chr8_3:50502187-50502206	Msa1293400:three_prime_UTR	45.0%
	TTACGTACAGCACCCAAACA+TGG	+	chr8_3:50502235-50502254	None:intergenic	45.0%
!	TGGGTGCTGTACGTAATGTT+AGG	-	chr8_3:50502239-50502258	Msa1293400:three_prime_UTR	45.0%
!	GGGTGCTGTACGTAATGTTA+GGG	-	chr8_3:50502240-50502259	Msa1293400:three_prime_UTR	45.0%
	ACTCTAGCACAACAAACTGG+TGG	+	chr8_3:50502274-50502293	None:intergenic	45.0%
	CCAACTCTAGCACAACAAAC+TGG	+	chr8_3:50502277-50502296	None:intergenic	45.0%
!!	CCAGTTTGTTGTGCTAGAGT+TGG	-	chr8_3:50502274-50502293	Msa1293400:three_prime_UTR	45.0%
	GGAACAGTGATAGCAATAGC+AGG	+	chr8_3:50502376-50502395	None:intergenic	45.0%
	CACATTTCTTTCCAGCAGGT+CGG	+	chr8_3:50502420-50502439	None:intergenic	45.0%
	CTTCCACATTTCTTTCCAGC+AGG	+	chr8_3:50502424-50502443	None:intergenic	45.0%
!!	TTAATTCAATTAAATTATAA+TGG	+	chr8_3:50503247-50503266	None:intergenic	5.0%
!!	TTAATTCAATTAAATTATAA+TGG	+	chr8_3:50503247-50503266	None:intergenic	5.0%
!!	ATTTTGGAAGCTGAGGCAGC+TGG	-	chr8_3:50502163-50502182	Msa1293400:three_prime_UTR	50.0%
	AACATTAGGAACCGACCTGC+TGG	-	chr8_3:50502406-50502425	Msa1293400:intron	50.0%
	CGACCTGCTGGAAAGAAATG+TGG	-	chr8_3:50502418-50502437	Msa1293400:intron	50.0%
	ACTTGTGTCGCAAGTAGCCT+TGG	-	chr8_3:50502576-50502595	Msa1293400:intron	50.0%
	CTTGTGTCGCAAGTAGCCTT+GGG	-	chr8_3:50502577-50502596	Msa1293400:intron	50.0%
!!	ATTTTGGAAGCTGAGGCAGC+TGG	-	chr8_3:50502163-50502182	Msa1293400:three_prime_UTR	50.0%
	AACATTAGGAACCGACCTGC+TGG	-	chr8_3:50502406-50502425	Msa1293400:intron	50.0%
	CGACCTGCTGGAAAGAAATG+TGG	-	chr8_3:50502418-50502437	Msa1293400:intron	50.0%
	ACTTGTGTCGCAAGTAGCCT+TGG	-	chr8_3:50502576-50502595	Msa1293400:intron	50.0%
	CTTGTGTCGCAAGTAGCCTT+GGG	-	chr8_3:50502577-50502596	Msa1293400:intron	50.0%
	AGGCAGCTGGAGAATGTGGA+AGG	-	chr8_3:50502176-50502195	Msa1293400:three_prime_UTR	55.0%
	CTAGCTGCTGCTGACCCAAT+TGG	+	chr8_3:50502205-50502224	None:intergenic	55.0%
	AGGCAGCTGGAGAATGTGGA+AGG	-	chr8_3:50502176-50502195	Msa1293400:three_prime_UTR	55.0%
	CTAGCTGCTGCTGACCCAAT+TGG	+	chr8_3:50502205-50502224	None:intergenic	55.0%
	GCTGAGGCAGCTGGAGAATG+TGG	-	chr8_3:50502172-50502191	Msa1293400:three_prime_UTR	60.0%
	GCTGAGGCAGCTGGAGAATG+TGG	-	chr8_3:50502172-50502191	Msa1293400:three_prime_UTR	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	50502025	50503385	50502025	ID=Msa1293400;Name=Msa1293400
chr8_3	mRNA	50502025	50503385	50502025	ID=Msa1293400-mRNA-1;Parent=Msa1293400;Name=Msa1293400-mRNA-1;_AED=0.25;_eAED=0.25;_QI=72\|1\|1\|1\|1\|1\|2\|344\|120
chr8_3	exon	50502025	50502385	50502025	ID=Msa1293400-mRNA-1:exon:14088;Parent=Msa1293400-mRNA-1
chr8_3	exon	50502968	50503385	50502968	ID=Msa1293400-mRNA-1:exon:14087;Parent=Msa1293400-mRNA-1
chr8_3	five_prime_UTR	50503314	50503385	50503314	ID=Msa1293400-mRNA-1:five_prime_utr;Parent=Msa1293400-mRNA-1
chr8_3	CDS	50502968	50503313	50502968	ID=Msa1293400-mRNA-1:cds;Parent=Msa1293400-mRNA-1
chr8_3	CDS	50502369	50502385	50502369	ID=Msa1293400-mRNA-1:cds;Parent=Msa1293400-mRNA-1
chr8_3	three_prime_UTR	50502025	50502368	50502025	ID=Msa1293400-mRNA-1:three_prime_utr;Parent=Msa1293400-mRNA-1

Gene Sequence	>Msa1293400 ATGGAGTTCAAGGTTAGAACTTTCCTAGCCATAGTGGTGATGTTTTTTTTGGCATCAAGTGTGTTAATTTTGGAAGCTGAGGCAGCTGGAGAATGTGGAAGGACACCAATTGGGTCAGCAGCAGCTAGTCTAAGTCCATGTTTGGGTGCTGTACGTAATGTTAGGGCAAAAGTTCCACCAGTTTGTTGTGCTAGAGTTGGTGCTTTGATTAGAACTTCTCCAAGATGTCTTTGTTCTGTTCTTTTGTCACCTATGGCAAAACAAGCTAAGATTAATCCTGCTATTGCTATCACTGTTCCTAAGAGATGCAACATTAGGAACCGACCTGCTGGAAAGAAATGTGGAAGGTACACACTTCCTTGA
Protein sequence	>Msa1293400 MEFKVRTFLAIVVMFFLASSVLILEAEAAGECGRTPIGSAAASLSPCLGAVRNVRAKVPPVCCARVGALIRTSPRCLCSVLLSPMAKQAKINPAIAITVPKRCNIRNRPAGKKCGRYTLP*