AlfalfaGEDB | Msa1410390

M. sativa cultivar zhongmu-4 / Msa1410390

KEGG: K09419
GO: GO:0000302 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006979 GO:0006986 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033554 GO:0034605 GO:0034620 GO:0035966 GO:0035967 GO:0036293 GO:0036294 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071310 GO:0071453 GO:0071456 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410390	sp\|P41152\|HSF30_SOLPE	34.865	370	178	10	48	378	2	347	1.19e-52	181

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410390	A0A396IIY0	87.943	423	7	3	1	380	1	422	0.0	741

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TFs/TRs:

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Gene ID	Type	Classification
Msa1410390	TF	HSF

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000910	Msa1410390	0.814145	1.788509e-51	-8.615850e-47
Msa0003220	Msa1410390	0.842464	2.544597e-58	-8.615850e-47
Msa0003270	Msa1410390	0.818658	1.747746e-52	-8.615850e-47
Msa0005270	Msa1410390	0.802300	5.988715e-49	-8.615850e-47
Msa0010770	Msa1410390	0.836448	9.304285e-57	-8.615850e-47
Msa0030300	Msa1410390	0.806081	9.782402e-50	-8.615850e-47
Msa0037120	Msa1410390	0.820903	5.361874e-53	-8.615850e-47
Msa0038870	Msa1410390	0.833667	4.674091e-56	-8.615850e-47
Msa0041690	Msa1410390	0.832891	7.295794e-56	-8.615850e-47
Msa0046560	Msa1410390	0.804757	1.853074e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410390	MtrunA17_Chr4g0071051	87.943	423	7	3	1	380	1	422	0.0	741
Msa1410390	MtrunA17_Chr3g0135831	34.085	355	164	11	76	378	37	373	5.90e-50	172
Msa1410390	MtrunA17_Chr7g0260331	40.909	242	106	5	76	280	40	281	1.45e-48	168
Msa1410390	MtrunA17_Chr1g0204741	39.184	245	108	6	56	264	11	250	1.33e-47	164
Msa1410390	MtrunA17_Chr5g0410881	42.051	195	73	3	82	236	29	223	1.71e-42	155
Msa1410390	MtrunA17_Chr4g0039891	40.845	213	84	5	84	257	11	220	2.37e-39	146
Msa1410390	MtrunA17_Chr1g0185131	37.500	232	102	5	68	263	7	231	6.97e-38	139
Msa1410390	MtrunA17_Chr8g0379701	36.364	198	90	3	84	245	11	208	1.51e-32	126
Msa1410390	MtrunA17_Chr2g0330821	29.537	281	152	6	14	260	8	276	7.66e-29	115
Msa1410390	MtrunA17_Chr6g0467041	33.054	239	111	6	85	294	22	240	4.57e-28	112

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410390	AT2G26150.1	33.634	333	140	9	76	368	35	326	7.42e-48	166
Msa1410390	AT2G26150.4	33.634	333	140	9	76	368	35	326	7.42e-48	166
Msa1410390	AT5G54070.1	42.857	231	104	7	78	281	63	292	4.07e-47	164
Msa1410390	AT3G22830.1	38.528	231	100	5	76	265	52	281	5.20e-46	162
Msa1410390	AT3G51910.1	42.929	198	93	6	76	254	20	216	1.88e-42	149
Msa1410390	AT5G16820.1	40.816	196	78	3	84	241	26	221	8.81e-41	150
Msa1410390	AT5G16820.2	40.816	196	78	3	84	241	26	221	8.81e-41	150
Msa1410390	AT4G17750.1	40.566	212	74	4	83	247	50	256	1.59e-39	147
Msa1410390	AT1G32330.2	37.333	225	99	4	78	260	30	254	9.40e-38	142
Msa1410390	AT1G32330.1	37.333	225	99	4	78	260	30	254	9.51e-38	142

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Find 72 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGCGGTGGCACACGG+TGG	0.747694	tig0023654:+31484	None:intergenic
AAACTGACCCGATTGTTTCA+TGG	0.421449	tig0023654:+31824	Msa1410390:CDS
AAATATGAACACTGGTAGAA+TGG	0.455673	tig0023654:+33934	Msa1410390:CDS
AACAAAATGGCGGTGGCACA+CGG	0.510707	tig0023654:+31481	None:intergenic
AACGTCTTCTTGAGAAACGG+TGG	0.656391	tig0023654:-31781	None:intergenic
AAGCACTCCATGAAACAATC+GGG	0.577813	tig0023654:-31831	None:intergenic
AAGGTACTAATGTTGATTAT+AGG	0.444857	tig0023654:+33852	Msa1410390:CDS
AAGTATAACAAACTGCATCA+AGG	0.578236	tig0023654:+33527	Msa1410390:CDS
AATGTTGATTATAGGAGTCA+GGG	0.582952	tig0023654:+33860	Msa1410390:CDS
AATTGGAAATTCTGAAGCTA+AGG	0.526195	tig0023654:+33621	Msa1410390:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCAACTCAATGCATGTT+TGG	-	tig0023654:32079-32098	None:intergenic	30.0%
TTCGCCATTTGAATTTATTC+TGG	+	tig0023654:32482-32501	Msa1410390:intron	30.0%
GGTAATTTCCCAGAAATAAT+GGG	-	tig0023654:32806-32825	None:intergenic	30.0%
TGGTAATTTCCCAGAAATAA+TGG	-	tig0023654:32807-32826	None:intergenic	30.0%
ATATCCCGCATGTTAATATT+TGG	+	tig0023654:32958-32977	Msa1410390:intron	30.0%
AAGTATAACAAACTGCATCA+AGG	+	tig0023654:33527-33546	Msa1410390:CDS	30.0%
AGATCAGAACATGTTGAAAT+TGG	+	tig0023654:33604-33623	Msa1410390:CDS	30.0%
TATTCAACTCACAAATGTTC+AGG	+	tig0023654:33664-33683	Msa1410390:CDS	30.0%
ATTATCTGCTCCATAAACAA+TGG	-	tig0023654:33758-33777	None:intergenic	30.0%
ACTTTGCAATCTGAATTGAA+TGG	+	tig0023654:33899-33918	Msa1410390:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0023654	gene	31487	34219	31487	ID=Msa1410390;Name=Msa1410390
tig0023654	mRNA	31487	34219	31487	ID=Msa1410390-mRNA-1;Parent=Msa1410390;Name=Msa1410390-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|0\|0\|3\|0\|380
tig0023654	exon	31487	31715	31487	ID=Msa1410390-mRNA-1:exon:26575;Parent=Msa1410390-mRNA-1
tig0023654	exon	31761	31963	31761	ID=Msa1410390-mRNA-1:exon:26576;Parent=Msa1410390-mRNA-1
tig0023654	exon	33509	34219	33509	ID=Msa1410390-mRNA-1:exon:26577;Parent=Msa1410390-mRNA-1
tig0023654	CDS	31487	31715	31487	ID=Msa1410390-mRNA-1:cds;Parent=Msa1410390-mRNA-1
tig0023654	CDS	31761	31963	31761	ID=Msa1410390-mRNA-1:cds;Parent=Msa1410390-mRNA-1
tig0023654	CDS	33509	34219	33509	ID=Msa1410390-mRNA-1:cds;Parent=Msa1410390-mRNA-1

Gene Sequence

>Msa1410390

ATGGCGGTGGCACACGGTGGTTACGACGGTGAAATCGGTGGCTACAATTCTCGCCTAATCCCATCAATAAAACCAGAGTTATTCAACGACAGTGCAAATTCAAGCTCTTACACCTACGACGACGTTTCAACCGAAAGAAAAGAATCAGTACTAACCGAAGAAGAAGAAGAAGAAGGTGCTACAGCTTCTCACGCAATTCATATCAAAGAAGAGGTTCTTGAAGAAGACGGGTTACATGAGGTTGGTCCACCACCGTTTCTCAAGAAGACGTTTGAAATGGTGGAGGATCCGGAAACTGACCCGATTGTTTCATGGAGTGCTTCTCGTGATAGTTTTGTTGTTTGGGATTCTCATGAATTCTCCAAAATTCTTCTTCCTAAATACTTTAAGCATAATAATTTTTCTAGCTTCATTCGCCAGCTCAACACCTATAACATAAGAAGGAGAAGCAAGTATAACAAACTGCATCAAGGAGGATTCAATTTGATGAATCCTTGCGTTGAATCTGAAGTGGAGAAACTGAAAAAAGATCAGAACATGTTGAAATTGGAAATTCTGAAGCTAAGGCAACAACAAGAGAATTCAAATATTCAACTCACAAATGTTCAGGAGCGAGTTCGGCGAGCAGAATCGAAGCAATATCAGATGCTATTTTTCCTCACCAAAATGGCCAAAAGGCCATTGTTTATGGAGCAGATAATTCAAAAGATTAAGCGAAAAGGAGAAGTTGATGCTAATATAGACATGGTTAAAAGACCTAGATTGCTTGGAACACAAGGTACTAATGTTGATTATAGGAGTCAGGGTCGTGAACAATTTGCTACTTTGCAATCTGAATTGAATGGACTTTTTCCTGAAAATATGAACACTGGTAGAATGGAACCACCTGTTTCTACTCCCATGGAAGATGGACTGTGTAGCTCTTTACATGACTTGAGGGCTTGTGGTGGTTCTAGACAAAGTGTTCAAGATGCTACATCTGCATATCATGTTATGTCAGAGAAGCTTTTGGGGGAAAATTCTGTTGTTGATGAAGAAATGGATGTGAATGACTCTAATATCTATCTTGAATTAGAGGATTTGATAAGTAAGCCAACAGATTGGGGTGGATCTGCTAGTGGATTGGTGGGACAAACTAGTTGA

Protein sequence

>Msa1410390

MAVAHGGYDGEIGGYNSRLIPSIKPELFNDSANSSSYTYDDVSTERKESVLTEEEEEEGATASHAIHIKEEVLEEDGLHEVGPPPFLKKTFEMVEDPETDPIVSWSASRDSFVVWDSHEFSKILLPKYFKHNNFSSFIRQLNTYNIRRRSKYNKLHQGGFNLMNPCVESEVEKLKKDQNMLKLEILKLRQQQENSNIQLTNVQERVRRAESKQYQMLFFLTKMAKRPLFMEQIIQKIKRKGEVDANIDMVKRPRLLGTQGTNVDYRSQGREQFATLQSELNGLFPENMNTGRMEPPVSTPMEDGLCSSLHDLRACGGSRQSVQDATSAYHVMSEKLLGENSVVDEEMDVNDSNIYLELEDLISKPTDWGGSASGLVGQTS*