AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1353590


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353590 MtrunA17_Chr1g0187861 93.923 181 11 0 1 181 1 181 2.86e-93 269
Msa1353590 MtrunA17_Chr7g0260751 42.038 157 88 2 20 176 36 189 9.95e-36 123
Msa1353590 MtrunA17_Chr1g0184741 45.695 151 79 2 21 171 30 177 1.07e-29 107
Msa1353590 MtrunA17_Chr5g0437751 38.462 156 95 1 8 163 37 191 2.55e-26 99.8
Msa1353590 MtrunA17_Chr2g0330441 32.298 161 105 3 1 161 15 171 7.06e-25 95.5
Msa1353590 MtrunA17_Chr4g0005191 34.395 157 102 1 8 164 45 200 8.71e-25 96.3
Msa1353590 MtrunA17_Chr6g0488551 32.450 151 95 4 5 155 23 166 4.96e-20 83.2
Msa1353590 MtrunA17_Chr1g0190601 46.154 91 46 2 25 115 37 124 2.50e-19 81.3
Msa1353590 MtrunA17_Chr8g0341921 34.667 150 95 2 6 155 18 164 8.70e-18 76.6
Msa1353590 MtrunA17_Chr2g0330451 33.529 170 106 3 2 167 15 181 9.53e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353590 AT1G04260.1 40.462 173 100 2 4 176 2 171 3.01e-40 135
Msa1353590 AT2G38360.1 35.897 156 99 1 8 163 40 194 2.15e-27 103
Msa1353590 AT1G55190.1 37.179 156 95 2 6 161 20 172 4.16e-27 101
Msa1353590 AT1G08770.1 32.759 174 112 2 12 181 37 209 2.97e-26 99.8
Msa1353590 AT3G56110.3 39.310 145 87 1 19 163 41 184 1.46e-23 92.8
Msa1353590 AT3G56110.1 39.310 145 87 1 19 163 41 184 1.46e-23 92.8
Msa1353590 AT3G56110.2 39.310 145 87 1 19 163 41 184 1.46e-23 92.8
Msa1353590 AT3G56110.4 39.310 145 87 1 19 163 41 184 1.46e-23 92.8
Msa1353590 AT5G01640.1 35.099 151 97 1 13 163 45 194 1.86e-22 90.5
Msa1353590 AT2G40380.1 36.424 151 95 1 21 171 45 194 2.82e-22 89.7
Msa1353590 AT1G17700.1 34.810 158 99 3 12 168 26 180 2.21e-21 86.7
Msa1353590 AT5G05380.1 37.821 156 96 1 8 163 33 187 2.54e-21 87.4
Msa1353590 AT5G05380.3 37.821 156 96 1 8 163 84 238 5.78e-21 87.4
Msa1353590 AT5G05380.4 37.821 156 96 1 8 163 76 230 6.06e-21 87.0
Msa1353590 AT5G05380.2 37.821 156 96 1 8 163 100 254 9.54e-21 87.0
Msa1353590 AT3G13710.1 30.857 175 104 5 6 176 20 181 9.97e-21 85.1
Msa1353590 AT3G13720.1 31.579 171 108 3 6 176 20 181 2.35e-20 84.0
Msa1353590 AT5G07110.1 33.099 142 94 1 23 164 45 185 2.41e-14 68.6
Msa1353590 AT4G29658.2 33.333 153 99 3 2 154 14 163 2.12e-13 65.9
Msa1353590 AT1G55640.1 30.935 139 93 2 17 155 33 168 2.67e-13 65.5

Find 56 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATGTGGTGGTGTCGGTTT+TGG 0.241307 tig0013832:-4290 Msa1353590:CDS
AAGTGCGGCTTCTTTGAAAT+TGG 0.320337 tig0013832:+4660 Msa1353590:intergenic
TCCAATGCGCGTGGTGGCTT+CGG 0.332193 tig0013832:+4564 Msa1353590:intergenic
ATGGAAAGGAGAGAGATGAT+AGG 0.345503 tig0013832:+4596 Msa1353590:intergenic
TCCTCTCCACGCGCCACCCT+TGG 0.363758 tig0013832:-4633 Msa1353590:CDS
CTCCTCCTTGTCGCTTTCGC+TGG 0.377058 tig0013832:-4451 Msa1353590:CDS
GAGGACGGATGTTGGTGGTT+TGG 0.395824 tig0013832:-4230 Msa1353590:CDS
TCCTTGTCGCTTTCGCTGGT+TGG 0.409335 tig0013832:-4447 Msa1353590:CDS
TGGAAAGGAGAGAGATGATA+GGG 0.418104 tig0013832:+4597 Msa1353590:intergenic
AGATGATAGGGAGAGGAATT+CGG 0.432739 tig0013832:+4609 Msa1353590:intergenic
TAATATCAAACAGGTGCATA+AGG 0.438615 tig0013832:+4157 Msa1353590:intergenic
GCGGGGATTGTTTGTTTGCA+TGG 0.445223 tig0013832:-4262 Msa1353590:CDS
TCCGAAGCCACCACGCGCAT+TGG 0.447170 tig0013832:-4565 Msa1353590:CDS
TCGTGTTGTTGTTGTGGCGC+TGG 0.481756 tig0013832:-4356 Msa1353590:CDS
AGATGAAGGTGTAGTTGAAG+AGG 0.484589 tig0013832:+4524 Msa1353590:intergenic
CGACACCAATAATATCAAAC+AGG 0.486498 tig0013832:+4148 Msa1353590:intergenic
AGGAGAGAGATGATAGGGAG+AGG 0.492521 tig0013832:+4602 Msa1353590:intergenic
TCCAAGGGTGGCGCGTGGAG+AGG 0.505040 tig0013832:+4632 Msa1353590:intergenic
GTGGCTTCGGAGAGGGAAGA+TGG 0.507798 tig0013832:+4577 Msa1353590:intergenic
TGATGATCGTGTTGTTGTTG+TGG 0.510697 tig0013832:-4362 Msa1353590:CDS
GGAGAACGATGGCGAGAGGA+TGG 0.514793 tig0013832:+4470 Msa1353590:intergenic
GAGTGGTGTGTGGGTGAATG+TGG 0.517878 tig0013832:-4305 Msa1353590:CDS
GTGGGTGAATGTGGTGGTGT+CGG 0.523922 tig0013832:-4296 Msa1353590:CDS
GCGCGTGGTGGCTTCGGAGA+GGG 0.524314 tig0013832:+4570 Msa1353590:intergenic
GATGGTAGACGAGGCTGATG+AGG 0.526706 tig0013832:+4488 Msa1353590:intergenic
GAGGATGAAGAGGAAGATGA+AGG 0.535008 tig0013832:+4510 Msa1353590:intergenic
GGCTGATGAGGAGGATGAAG+AGG 0.545059 tig0013832:+4500 Msa1353590:intergenic
ATGCTAGCTACTGATACTGC+TGG 0.564776 tig0013832:-4181 Msa1353590:CDS
GATGATTATGAATCGCCGTA+TGG 0.570258 tig0013832:-4208 Msa1353590:CDS
TTCGGAGAGGGAAGATGGAA+AGG 0.572285 tig0013832:+4582 Msa1353590:intergenic
AGGGAGAGGAATTCGGTCCA+AGG 0.574199 tig0013832:+4616 Msa1353590:intergenic
GTTTGCATGGTGCGTTGAGG+AGG 0.576839 tig0013832:-4249 Msa1353590:CDS
GCAGTATCAGTAGCTAGCAT+AGG 0.582398 tig0013832:+4184 Msa1353590:intergenic
GCATGGTGCGTTGAGGAGGA+CGG 0.586209 tig0013832:-4245 Msa1353590:CDS
ACGACGTTGAACAGCGACAA+AGG 0.588894 tig0013832:+4391 Msa1353590:intergenic
GAACAGCGACAAAGGCTCAT+CGG 0.591879 tig0013832:+4399 Msa1353590:intergenic
TTGAGGAGGACGGATGTTGG+TGG 0.594756 tig0013832:-4235 Msa1353590:CDS
GGGAGAGGAATTCGGTCCAA+GGG 0.594820 tig0013832:+4617 Msa1353590:intergenic
AGAGGAATTCGGTCCAAGGG+TGG 0.596200 tig0013832:+4620 Msa1353590:intergenic
TGTTGTTGTTGTGGCGCTGG+TGG 0.598452 tig0013832:-4353 Msa1353590:CDS
GGTAGACGAGGCTGATGAGG+AGG 0.602901 tig0013832:+4491 Msa1353590:intergenic
GCGTTGAGGAGGACGGATGT+TGG 0.603625 tig0013832:-4238 Msa1353590:CDS
ACCAACCAGCGAAAGCGACA+AGG 0.609728 tig0013832:+4446 Msa1353590:intergenic
TGGTGTGTGGGTGAATGTGG+TGG 0.613899 tig0013832:-4302 Msa1353590:CDS
TTTGTCGCTGTTCAACGTCG+TGG 0.617871 tig0013832:-4389 Msa1353590:CDS
GTAGCTAGCATAGGACCATA+CGG 0.624008 tig0013832:+4193 Msa1353590:intergenic
CGTGGAGAGGATTGAAAGTG+CGG 0.624477 tig0013832:+4645 Msa1353590:intergenic
GTCGCTGTTCAACGTCGTGG+CGG 0.633869 tig0013832:-4386 Msa1353590:CDS
AGGAGGAGAACGATGGCGAG+AGG 0.638618 tig0013832:+4466 Msa1353590:intergenic
TGCGCGTGGTGGCTTCGGAG+AGG 0.641439 tig0013832:+4569 Msa1353590:intergenic
AGCGACAAGGAGGAGAACGA+TGG 0.650682 tig0013832:+4459 Msa1353590:intergenic
ATTGATTCCAATGCGCGTGG+TGG 0.675368 tig0013832:+4558 Msa1353590:intergenic
GAGATTGATTCCAATGCGCG+TGG 0.679657 tig0013832:+4555 Msa1353590:intergenic
AACCAGCGAAAGCGACAAGG+AGG 0.690375 tig0013832:+4449 Msa1353590:intergenic
TGGCGAGAGGATGGTAGACG+AGG 0.695341 tig0013832:+4479 Msa1353590:intergenic
TTTGTTTGCATGGTGCGTTG+AGG 0.700659 tig0013832:-4252 Msa1353590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTGTTGTTTTTGTGAG+TGG - tig0013832:4525-4544 Msa1353590:CDS 30.0%
!! AAGTGCGGCTTCTTTGAAAT+TGG + tig0013832:4190-4209 Msa0988860:intergenic 40.0%
AGATGATAGGGAGAGGAATT+CGG + tig0013832:4241-4260 Msa1353590:intergenic 40.0%
TGGAAAGGAGAGAGATGATA+GGG + tig0013832:4253-4272 Msa1353590:intergenic 40.0%
ATGGAAAGGAGAGAGATGAT+AGG + tig0013832:4254-4273 Msa1353590:intergenic 40.0%
! AGATGAAGGTGTAGTTGAAG+AGG + tig0013832:4326-4345 Msa1353590:intergenic 40.0%
! TGATGATCGTGTTGTTGTTG+TGG - tig0013832:4485-4504 Msa1353590:CDS 40.0%
!!! TTGTTTTTGTGAGTGGTGTG+TGG - tig0013832:4532-4551 Msa1353590:CDS 40.0%
!! TGTTTTTGTGAGTGGTGTGT+GGG - tig0013832:4533-4552 Msa1353590:CDS 40.0%
GATGATTATGAATCGCCGTA+TGG - tig0013832:4639-4658 Msa1353590:CDS 40.0%
GAGGATGAAGAGGAAGATGA+AGG + tig0013832:4340-4359 Msa1353590:intergenic 45.0%
TTTGTTTGCATGGTGCGTTG+AGG - tig0013832:4595-4614 Msa1353590:CDS 45.0%
GTAGCTAGCATAGGACCATA+CGG + tig0013832:4657-4676 Msa1353590:intergenic 45.0%
GCAGTATCAGTAGCTAGCAT+AGG + tig0013832:4666-4685 Msa1353590:intergenic 45.0%
ATGCTAGCTACTGATACTGC+TGG - tig0013832:4666-4685 Msa1353590:CDS 45.0%
CGTGGAGAGGATTGAAAGTG+CGG + tig0013832:4205-4224 Msa0988860:intergenic 50.0%
AGGAGAGAGATGATAGGGAG+AGG + tig0013832:4248-4267 Msa1353590:intergenic 50.0%
TTCGGAGAGGGAAGATGGAA+AGG + tig0013832:4268-4287 Msa1353590:intergenic 50.0%
! ATTGATTCCAATGCGCGTGG+TGG + tig0013832:4292-4311 Msa1353590:intergenic 50.0%
! GAGATTGATTCCAATGCGCG+TGG + tig0013832:4295-4314 Msa1353590:intergenic 50.0%
GAACAGCGACAAAGGCTCAT+CGG + tig0013832:4451-4470 Msa1353590:intergenic 50.0%
ACGACGTTGAACAGCGACAA+AGG + tig0013832:4459-4478 Msa1353590:intergenic 50.0%
TTTGTCGCTGTTCAACGTCG+TGG - tig0013832:4458-4477 Msa1353590:CDS 50.0%
!!! GAATGTGGTGGTGTCGGTTT+TGG - tig0013832:4557-4576 Msa1353590:CDS 50.0%
GCGGGGATTGTTTGTTTGCA+TGG - tig0013832:4585-4604 Msa1353590:CDS 50.0%
AGAGGAATTCGGTCCAAGGG+TGG + tig0013832:4230-4249 Msa1353590:intergenic 55.0%
GGGAGAGGAATTCGGTCCAA+GGG + tig0013832:4233-4252 Msa1353590:intergenic 55.0%
AGGGAGAGGAATTCGGTCCA+AGG + tig0013832:4234-4253 Msa1353590:intergenic 55.0%
GGCTGATGAGGAGGATGAAG+AGG + tig0013832:4350-4369 Msa1353590:intergenic 55.0%
GATGGTAGACGAGGCTGATG+AGG + tig0013832:4362-4381 Msa1353590:intergenic 55.0%
AGCGACAAGGAGGAGAACGA+TGG + tig0013832:4391-4410 Msa1353590:intergenic 55.0%
AACCAGCGAAAGCGACAAGG+AGG + tig0013832:4401-4420 Msa1353590:intergenic 55.0%
!! TCCTTGTCGCTTTCGCTGGT+TGG - tig0013832:4400-4419 Msa1353590:CDS 55.0%
ACCAACCAGCGAAAGCGACA+AGG + tig0013832:4404-4423 Msa1353590:intergenic 55.0%
! TCGTGTTGTTGTTGTGGCGC+TGG - tig0013832:4491-4510 Msa1353590:CDS 55.0%
! TGTTGTTGTTGTGGCGCTGG+TGG - tig0013832:4494-4513 Msa1353590:CDS 55.0%
GAGTGGTGTGTGGGTGAATG+TGG - tig0013832:4542-4561 Msa1353590:CDS 55.0%
TGGTGTGTGGGTGAATGTGG+TGG - tig0013832:4545-4564 Msa1353590:CDS 55.0%
! GTGGGTGAATGTGGTGGTGT+CGG - tig0013832:4551-4570 Msa1353590:CDS 55.0%
!!! GGTGGTGTCGGTTTTGGTTG+CGG - tig0013832:4563-4582 Msa1353590:CDS 55.0%
GTTTGCATGGTGCGTTGAGG+AGG - tig0013832:4598-4617 Msa1353590:CDS 55.0%
TTGAGGAGGACGGATGTTGG+TGG - tig0013832:4612-4631 Msa1353590:CDS 55.0%
!! GAGGACGGATGTTGGTGGTT+TGG - tig0013832:4617-4636 Msa1353590:CDS 55.0%
GTGGCTTCGGAGAGGGAAGA+TGG + tig0013832:4273-4292 Msa1353590:intergenic 60.0%
TCCAATGCGCGTGGTGGCTT+CGG + tig0013832:4286-4305 Msa1353590:intergenic 60.0%
GGTAGACGAGGCTGATGAGG+AGG + tig0013832:4359-4378 Msa1353590:intergenic 60.0%
TGGCGAGAGGATGGTAGACG+AGG + tig0013832:4371-4390 Msa1353590:intergenic 60.0%
GGAGAACGATGGCGAGAGGA+TGG + tig0013832:4380-4399 Msa1353590:intergenic 60.0%
AGGAGGAGAACGATGGCGAG+AGG + tig0013832:4384-4403 Msa1353590:intergenic 60.0%
!! CTCCTCCTTGTCGCTTTCGC+TGG - tig0013832:4396-4415 Msa1353590:CDS 60.0%
GTCGCTGTTCAACGTCGTGG+CGG - tig0013832:4461-4480 Msa1353590:CDS 60.0%
!!! GGTGTCGGTTTTGGTTGCGG+CGG - tig0013832:4566-4585 Msa1353590:CDS 60.0%
!!! GTGTCGGTTTTGGTTGCGGC+GGG - tig0013832:4567-4586 Msa1353590:CDS 60.0%
!!! TGTCGGTTTTGGTTGCGGCG+GGG - tig0013832:4568-4587 Msa1353590:CDS 60.0%
GCATGGTGCGTTGAGGAGGA+CGG - tig0013832:4602-4621 Msa1353590:CDS 60.0%
GCGTTGAGGAGGACGGATGT+TGG - tig0013832:4609-4628 Msa1353590:CDS 60.0%
TCCGAAGCCACCACGCGCAT+TGG - tig0013832:4282-4301 Msa1353590:CDS 65.0%
TCCTCTCCACGCGCCACCCT+TGG - tig0013832:4214-4233 Msa1353590:CDS 70.0%
TCCAAGGGTGGCGCGTGGAG+AGG + tig0013832:4218-4237 Msa1353590:intergenic 70.0%
TTCGGTCCAAGGGTGGCGCG+TGG + tig0013832:4223-4242 Msa1353590:intergenic 70.0%
GCGCGTGGTGGCTTCGGAGA+GGG + tig0013832:4280-4299 Msa1353590:intergenic 70.0%
TGCGCGTGGTGGCTTCGGAG+AGG + tig0013832:4281-4300 Msa1353590:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0013832 gene 4162 4707 4162 ID=Msa1353590;Name=Msa1353590
tig0013832 mRNA 4162 4707 4162 ID=Msa1353590-mRNA-1;Parent=Msa1353590;Name=Msa1353590-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|181
tig0013832 exon 4162 4707 4162 ID=Msa1353590-mRNA-1:exon:8743;Parent=Msa1353590-mRNA-1
tig0013832 CDS 4162 4707 4162 ID=Msa1353590-mRNA-1:cds;Parent=Msa1353590-mRNA-1
Gene Sequence

>Msa1353590

ATGTCTTCTTCTACTGAAACACTCTCCAATTTCAAAGAAGCCGCACTTTCAATCCTCTCCACGCGCCACCCTTGGACCGAATTCCTCTCCCTATCATCTCTCTCCTTTCCATCTTCCCTCTCCGAAGCCACCACGCGCATTGGAATCAATCTCACGCGCTTCCTCTTCAACTACACCTTCATCTTCCTCTTCATCCTCCTCATCAGCCTCGTCTACCATCCTCTCGCCATCGTTCTCCTCCTTGTCGCTTTCGCTGGTTGGTACTTTCTGTTCTTCTCACGTGACTCCGATGAGCCTTTGTCGCTGTTCAACGTCGTGGCGGTTGATGATCGTGTTGTTGTTGTGGCGCTGGTGGTTTTTAGTTTGTTTGTTGTTTTTGTGAGTGGTGTGTGGGTGAATGTGGTGGTGTCGGTTTTGGTTGCGGCGGGGATTGTTTGTTTGCATGGTGCGTTGAGGAGGACGGATGTTGGTGGTTTGGATGATTATGAATCGCCGTATGGTCCTATGCTAGCTACTGATACTGCTGGTCCTTATGCACCTGTTTGA

Protein sequence

>Msa1353590

MSSSTETLSNFKEAALSILSTRHPWTEFLSLSSLSFPSSLSEATTRIGINLTRFLFNYTFIFLFILLISLVYHPLAIVLLLVAFAGWYFLFFSRDSDEPLSLFNVVAVDDRVVVVALVVFSLFVVFVSGVWVNVVVSVLVAAGIVCLHGALRRTDVGGLDDYESPYGPMLATDTAGPYAPV*