AlfalfaGEDB | Msa1224400

M. sativa cultivar zhongmu-4 / Msa1224400

KEGG: K03955
GO: GO:0000035 GO:0000036 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009245 GO:0009247 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016053 GO:0019637 GO:0019752 GO:0019842 GO:0031177 GO:0032787 GO:0033218 GO:0036094 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0044620 GO:0046394 GO:0046467 GO:0046493 GO:0048037 GO:0051192 GO:0071704 GO:0072330 GO:0072341 GO:0090407 GO:0140104 GO:1901135 GO:1901137 GO:1901269 GO:1901271 GO:1901576 GO:1903509
PFAM: PF00550.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224400	PNY14930.1	95.161	124	6	0	1	124	1	124	1.61e-79	239

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224400	sp\|Q9FGJ4\|ACPM3_ARATH	56.693	127	51	1	1	123	1	127	1.07e-41	137

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008330	Msa1224400	0.810210	1.290742e-50	-8.615850e-47
Msa0036150	Msa1224400	0.835146	1.988739e-56	-8.615850e-47
Msa0040190	Msa1224400	0.825586	4.323217e-54	-8.615850e-47
Msa0075850	Msa1224400	0.806702	7.234357e-50	-8.615850e-47
Msa0087880	Msa1224400	0.802821	4.678078e-49	-8.615850e-47
Msa0619330	Msa1224400	0.823025	1.729054e-53	-8.615850e-47
Msa0621890	Msa1224400	0.812277	4.596752e-51	-8.615850e-47
Msa0647000	Msa1224400	0.803233	3.844472e-49	-8.615850e-47
Msa0678240	Msa1224400	0.800928	1.145023e-48	-8.615850e-47
Msa0694690	Msa1224400	-0.804358	2.244449e-49	-8.615850e-47
Msa0701770	Msa1224400	0.807089	5.994229e-50	-8.615850e-47
Msa0725480	Msa1224400	-0.804861	1.763030e-49	-8.615850e-47
Msa0739570	Msa1224400	0.837626	4.651026e-57	-8.615850e-47
Msa0749550	Msa1224400	0.811313	7.452501e-51	-8.615850e-47
Msa0749610	Msa1224400	0.803924	2.763911e-49	-8.615850e-47
Msa0756480	Msa1224400	0.827814	1.270869e-54	-8.615850e-47
Msa0766100	Msa1224400	-0.809764	1.610503e-50	-8.615850e-47
Msa0782330	Msa1224400	0.819363	1.207777e-52	-8.615850e-47
Msa0792510	Msa1224400	0.829821	4.152644e-55	-8.615850e-47
Msa0815380	Msa1224400	0.806009	1.012975e-49	-8.615850e-47
Msa0835510	Msa1224400	0.834259	3.323664e-56	-8.615850e-47
Msa0837990	Msa1224400	0.805718	1.165943e-49	-8.615850e-47
Msa0134550	Msa1224400	0.824235	9.010720e-54	-8.615850e-47
Msa0141110	Msa1224400	-0.800798	1.217216e-48	-8.615850e-47
Msa0141670	Msa1224400	0.801745	7.788679e-49	-8.615850e-47
Msa0164660	Msa1224400	0.803909	2.784049e-49	-8.615850e-47
Msa0168390	Msa1224400	0.814584	1.430171e-51	-8.615850e-47
Msa0189050	Msa1224400	0.827541	1.477637e-54	-8.615850e-47
Msa0211010	Msa1224400	0.804523	2.073571e-49	-8.615850e-47
Msa0227500	Msa1224400	0.803990	2.677997e-49	-8.615850e-47
Msa0231310	Msa1224400	0.828652	7.981606e-55	-8.615850e-47
Msa0365360	Msa1224400	0.829861	4.062045e-55	-8.615850e-47
Msa0371610	Msa1224400	0.827784	1.292283e-54	-8.615850e-47
Msa0374860	Msa1224400	-0.802649	5.075007e-49	-8.615850e-47
Msa0404870	Msa1224400	0.805326	1.409029e-49	-8.615850e-47
Msa0409820	Msa1224400	0.805352	1.391767e-49	-8.615850e-47
Msa0413740	Msa1224400	0.821062	4.929249e-53	-8.615850e-47
Msa0418000	Msa1224400	0.845698	3.452660e-59	-8.615850e-47
Msa0431710	Msa1224400	0.808294	3.323946e-50	-8.615850e-47
Msa0436900	Msa1224400	0.812088	5.054223e-51	-8.615850e-47
Msa0457370	Msa1224400	0.815742	7.911163e-52	-8.615850e-47
Msa0458790	Msa1224400	0.809423	1.906147e-50	-8.615850e-47
Msa0466100	Msa1224400	0.808336	3.256456e-50	-8.615850e-47
Msa0475830	Msa1224400	0.820382	7.064114e-53	-8.615850e-47
Msa0981010	Msa1224400	0.806268	8.931314e-50	-8.615850e-47
Msa0988500	Msa1224400	0.800485	1.410061e-48	-8.615850e-47
Msa1009000	Msa1224400	0.821508	3.889810e-53	-8.615850e-47
Msa1018720	Msa1224400	0.808659	2.777824e-50	-8.615850e-47
Msa1064420	Msa1224400	0.816092	6.606773e-52	-8.615850e-47
Msa1069300	Msa1224400	0.805073	1.591598e-49	-8.615850e-47
Msa1079610	Msa1224400	0.816555	5.206550e-52	-8.615850e-47
Msa1081600	Msa1224400	0.860880	1.516594e-63	-8.615850e-47
Msa1098560	Msa1224400	0.804362	2.240453e-49	-8.615850e-47
Msa0257930	Msa1224400	-0.814168	1.767340e-51	-8.615850e-47
Msa0286790	Msa1224400	0.823253	1.529680e-53	-8.615850e-47
Msa0304990	Msa1224400	0.833296	5.787400e-56	-8.615850e-47
Msa0306130	Msa1224400	0.843196	1.626133e-58	-8.615850e-47
Msa0309160	Msa1224400	0.832376	9.790909e-56	-8.615850e-47
Msa0320540	Msa1224400	0.809449	1.881614e-50	-8.615850e-47
Msa0358710	Msa1224400	0.804527	2.070155e-49	-8.615850e-47
Msa1125040	Msa1224400	0.810193	1.301856e-50	-8.615850e-47
Msa1166370	Msa1224400	0.848394	6.302972e-60	-8.615850e-47
Msa1201990	Msa1224400	0.811686	6.184535e-51	-8.615850e-47
Msa1208070	Msa1224400	0.803382	3.580119e-49	-8.615850e-47
Msa1211370	Msa1224400	0.808202	3.476917e-50	-8.615850e-47
Msa1220230	Msa1224400	0.801175	1.019255e-48	-8.615850e-47
Msa1220710	Msa1224400	0.818756	1.660266e-52	-8.615850e-47
Msa1221200	Msa1224400	-0.813770	2.163126e-51	-8.615850e-47
Msa1221570	Msa1224400	-0.800616	1.325470e-48	-8.615850e-47
Msa1224400	Msa1238480	0.807920	3.993525e-50	-8.615850e-47
Msa1224400	Msa1244830	0.805814	1.112993e-49	-8.615850e-47
Msa1224400	Msa1254770	0.817474	3.236214e-52	-8.615850e-47
Msa1224400	Msa1254800	0.816301	5.934692e-52	-8.615850e-47
Msa1224400	Msa1265660	0.804583	2.015181e-49	-8.615850e-47
Msa1224400	Msa1287160	0.812971	3.241147e-51	-8.615850e-47
Msa1224400	Msa1287340	0.808168	3.535274e-50	-8.615850e-47
Msa1224400	Msa1295010	0.802991	4.314164e-49	-8.615850e-47
Msa1224400	Msa1303570	0.803791	2.944508e-49	-8.615850e-47
Msa1224400	Msa1303640	0.801203	1.005958e-48	-8.615850e-47
Msa1224400	Msa1326000	0.805823	1.108039e-49	-8.615850e-47
Msa1224400	Msa1329990	0.809825	1.562427e-50	-8.615850e-47
Msa1224400	Msa1332450	0.986913	2.098991e-168	-8.615850e-47
Msa1224400	Msa1337190	0.949966	4.347688e-108	-8.615850e-47
Msa1224400	Msa1355260	0.850636	1.493411e-60	-8.615850e-47
Msa1224400	Msa1373950	0.833213	6.067547e-56	-8.615850e-47
Msa1224400	Msa1387090	0.819693	1.015775e-52	-8.615850e-47
Msa1224400	Msa1389120	0.996548	5.901795e-229	-8.615850e-47
Msa1224400	Msa1390410	0.833517	5.094975e-56	-8.615850e-47
Msa1224400	Msa1397540	0.803969	2.704919e-49	-8.615850e-47
Msa1224400	Msa1399430	0.801783	7.651656e-49	-8.615850e-47
Msa1224400	Msa1409520	0.813903	2.022218e-51	-8.615850e-47
Msa1224400	Msa1423080	0.803066	4.161846e-49	-8.615850e-47
Msa0498920	Msa1224400	0.812426	4.264693e-51	-8.615850e-47
Msa0504630	Msa1224400	0.824634	7.257787e-54	-8.615850e-47
Msa0505390	Msa1224400	0.814687	1.357371e-51	-8.615850e-47
Msa0511890	Msa1224400	0.801490	8.784209e-49	-8.615850e-47
Msa0524050	Msa1224400	0.802245	6.148157e-49	-8.615850e-47
Msa0535020	Msa1224400	0.809401	1.926738e-50	-8.615850e-47
Msa0535050	Msa1224400	0.816933	4.283237e-52	-8.615850e-47
Msa0542010	Msa1224400	0.800045	1.732340e-48	-8.615850e-47
Msa0557680	Msa1224400	0.801119	1.046608e-48	-8.615850e-47
Msa0575250	Msa1224400	0.818922	1.522059e-52	-8.615850e-47
Msa0591780	Msa1224400	0.825534	4.449588e-54	-8.615850e-47
Msa0855520	Msa1224400	0.823870	1.097503e-53	-8.615850e-47
Msa0860270	Msa1224400	0.850639	1.490711e-60	-8.615850e-47
Msa0880080	Msa1224400	0.806171	9.363439e-50	-8.615850e-47
Msa0921630	Msa1224400	0.805049	1.610437e-49	-8.615850e-47
Msa0951720	Msa1224400	0.807253	5.532117e-50	-8.615850e-47
Msa0960270	Msa1224400	0.815707	8.054711e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224400	MtrunA17_Chr4g0076301	94.355	124	7	1	124	1	124	6.58e-83	238
Msa1224400	MtrunA17_Chr7g0249561	50.649	77	38	47	123	54	130	2.02e-21	82.8
Msa1224400	MtrunA17_Chr7g0249801	48.649	74	38	47	120	43	116	2.03e-21	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224400	AT5G47630.1	56.693	127	51	1	1	123	1	127	1.09e-42	137
Msa1224400	AT5G47630.2	56.693	127	51	1	1	123	1	127	1.09e-42	137
Msa1224400	AT5G47630.3	56.693	127	51	1	1	123	1	127	1.09e-42	137
Msa1224400	AT1G65290.1	50.649	77	38	0	47	123	50	126	3.52e-21	82.4
Msa1224400	AT2G44620.1	41.000	100	46	1	17	116	29	115	6.34e-21	81.6

Find 34 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCAACAAAACTTCTCTTTA+TGG	0.154842	8_1:-89339850	Msa1224410:three_prime_UTR
GGATAGGGTTGAGCTTATTA+TGG	0.307345	8_1:+89339671	Msa1224400:CDS
AGTAAAAGTGAAAATAATAT+TGG	0.326289	8_1:-89339214	None:intergenic
AGCTTATCTGCTTTCTCATC+AGG	0.361227	8_1:-89339723	Msa1224410:three_prime_UTR
GCAAAACAATTGAAAAGTTA+TGG	0.376214	8_1:-89339809	Msa1224410:three_prime_UTR
ATATGCCGACAAAATTTGAT+TGG	0.384777	8_1:-89338307	None:intergenic
CACTCACTGGGAGATTCGTT+TGG	0.407167	8_1:-89338330	None:intergenic
TCAGGTGTCATGCTTACATT+GGG	0.419498	8_1:-89339961	Msa1224410:three_prime_UTR
ATCAGGTGTCATGCTTACAT+TGG	0.439491	8_1:-89339962	Msa1224410:three_prime_UTR
CATAATCACATTATCATCCT+TGG	0.439969	8_1:-89339377	Msa1224410:three_prime_UTR
GGACTTAAACCTGGACAGCT+TGG	0.459469	8_1:+89339650	Msa1224400:CDS
TGATTATGCATTTGAGATGT+TGG	0.460487	8_1:+89339392	Msa1224400:CDS
TGAGAAGGTCAACTGAAAGA+TGG	0.475010	8_1:+89339347	Msa1224400:CDS
GTCTAGTATCGGATCAGAGC+TGG	0.475971	8_1:-89339431	Msa1224410:three_prime_UTR
TGTCATGCTTACATTGGGGT+GGG	0.479726	8_1:-89339956	Msa1224410:three_prime_UTR
AGATGTTGGTGCTCTTCAGC+AGG	0.487417	8_1:+89339406	Msa1224400:CDS
CAACCCTATCCAAGCTGTCC+AGG	0.498453	8_1:-89339659	Msa1224410:three_prime_UTR
TGCCACTTTGAATTAGCACT+TGG	0.508091	8_1:+89339262	Msa1224400:five_prime_UTR
TTCAATAAAGCATAACTCAA+CGG	0.513754	8_1:-89340032	Msa1224410:three_prime_UTR
TAAACCTGGACAGCTTGGAT+AGG	0.517739	8_1:+89339655	Msa1224400:CDS
GTGTCATGCTTACATTGGGG+TGG	0.521344	8_1:-89339957	Msa1224410:three_prime_UTR
TCTTGACCAGTCTGATTACT+CGG	0.521850	8_1:-89339453	Msa1224410:three_prime_UTR
AAACCTGGACAGCTTGGATA+GGG	0.522123	8_1:+89339656	Msa1224400:CDS
CTGATTACTCGGTCTAGTAT+CGG	0.536163	8_1:-89339442	Msa1224410:three_prime_UTR
GTCATGCTTACATTGGGGTG+GGG	0.536900	8_1:-89339955	Msa1224410:three_prime_UTR
GGCTATGTACTTTGCAACAT+CGG	0.551029	8_1:-89339757	Msa1224410:three_prime_UTR
TTTCTCATCAGGAATTTCGA+TGG	0.580152	8_1:-89339712	Msa1224410:three_prime_UTR
TGATAAAATTAATGCAGCAA+AGG	0.580568	8_1:+89339480	Msa1224400:CDS
TGTACGAAAATAGAAAAGCA+AGG	0.585420	8_1:-89339995	Msa1224410:three_prime_UTR
GTACGAAAATAGAAAAGCAA+GGG	0.606712	8_1:-89339994	Msa1224410:three_prime_UTR
TAAGTCATGTGAATTTGAGA+AGG	0.613186	8_1:+89339332	Msa1224400:CDS
ATCCAAGTGCTAATTCAAAG+TGG	0.625412	8_1:-89339264	None:intergenic
ACTAGACCGAGTAATCAGAC+TGG	0.680991	8_1:+89339447	Msa1224400:CDS
CAGGTGTCATGCTTACATTG+GGG	0.762397	8_1:-89339960	Msa1224410:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGTCATTTTTTATTTTTTTT+TGG	+	chr8_1:89338669-89338688	Msa1224400:intron	10.0%
!!!	GTCATTTTTTATTTTTTTTT+GGG	+	chr8_1:89338670-89338689	Msa1224400:intron	10.0%
!!!	TCATTTTTTATTTTTTTTTG+GGG	+	chr8_1:89338671-89338690	Msa1224400:intron	10.0%
!!!	TGTCATTTTTTATTTTTTTT+TGG	+	chr8_1:89338669-89338688	Msa1224400:intron	10.0%
!!!	GTCATTTTTTATTTTTTTTT+GGG	+	chr8_1:89338670-89338689	Msa1224400:intron	10.0%
!!!	TCATTTTTTATTTTTTTTTG+GGG	+	chr8_1:89338671-89338690	Msa1224400:intron	10.0%
!!!	CATTTTTTATTTTTTTTTGG+GGG	+	chr8_1:89338672-89338691	Msa1224400:intron	15.0%
!!!	GATTTTGTTATTTTTAATGA+AGG	+	chr8_1:89339079-89339098	Msa1224400:intron	15.0%
!!	AGTAAAAGTGAAAATAATAT+TGG	-	chr8_1:89339217-89339236	None:intergenic	15.0%
!!	TGACATAAAAAAATAAGAAA+AGG	-	chr8_1:89339935-89339954	Msa1224410:three_prime_UTR	15.0%
!!!	CATTTTTTATTTTTTTTTGG+GGG	+	chr8_1:89338672-89338691	Msa1224400:intron	15.0%
!!!	GATTTTGTTATTTTTAATGA+AGG	+	chr8_1:89339079-89339098	Msa1224400:intron	15.0%
!!	AGTAAAAGTGAAAATAATAT+TGG	-	chr8_1:89339217-89339236	None:intergenic	15.0%
!!	TGACATAAAAAAATAAGAAA+AGG	-	chr8_1:89339935-89339954	Msa1224410:three_prime_UTR	15.0%
!!!	TTAATCCAATCAAATTTTGT+CGG	+	chr8_1:89338302-89338321	Msa1224400:five_prime_UTR	20.0%
!!	AATTCAACAATAGAAGTAAA+TGG	+	chr8_1:89338733-89338752	Msa1224400:intron	20.0%
!!	AAATAAAAAGTAGAAGTCAT+AGG	-	chr8_1:89339030-89339049	None:intergenic	20.0%
!!	TGCATGTTTATTTATATGAA+TGG	+	chr8_1:89339049-89339068	Msa1224400:intron	20.0%
!!!	TATTTTCTTTTGACTATAAC+TGG	-	chr8_1:89339510-89339529	Msa1224410:three_prime_UTR	20.0%
!!!	ATTGGTATATATTTTTGTGT+AGG	+	chr8_1:89339602-89339621	Msa1224400:intron	20.0%
!!!	TTAATCCAATCAAATTTTGT+CGG	+	chr8_1:89338302-89338321	Msa1224400:five_prime_UTR	20.0%
!!	AATTCAACAATAGAAGTAAA+TGG	+	chr8_1:89338733-89338752	Msa1224400:intron	20.0%
!!	AAATAAAAAGTAGAAGTCAT+AGG	-	chr8_1:89339030-89339049	None:intergenic	20.0%
!!	TGCATGTTTATTTATATGAA+TGG	+	chr8_1:89339049-89339068	Msa1224400:intron	20.0%
!!!	TATTTTCTTTTGACTATAAC+TGG	-	chr8_1:89339510-89339529	Msa1224410:three_prime_UTR	20.0%
!!!	ATTGGTATATATTTTTGTGT+AGG	+	chr8_1:89339602-89339621	Msa1224400:intron	20.0%
!	TACAAGACATAAAGAAGAAA+AGG	-	chr8_1:89338367-89338386	None:intergenic	25.0%
!!!	ATTTTTTTTTGGGGGTATAT+AGG	+	chr8_1:89338680-89338699	Msa1224400:intron	25.0%
!!!	TTTTTTTTTGGGGGTATATA+GGG	+	chr8_1:89338681-89338700	Msa1224400:intron	25.0%
!	AGAAAACACAAATCCTTTAT+AGG	+	chr8_1:89338846-89338865	Msa1224400:intron	25.0%
!	TCATTAGTGAAAACCTATAA+AGG	-	chr8_1:89338862-89338881	None:intergenic	25.0%
!	TGATAAAATTAATGCAGCAA+AGG	+	chr8_1:89339480-89339499	Msa1224400:CDS	25.0%
!	GCAAAACAATTGAAAAGTTA+TGG	-	chr8_1:89339812-89339831	Msa1224410:three_prime_UTR	25.0%
!	AAAAAATAAGAAAAGGAGAC+AGG	-	chr8_1:89339928-89339947	Msa1224410:three_prime_UTR	25.0%
!	TACAAGACATAAAGAAGAAA+AGG	-	chr8_1:89338367-89338386	None:intergenic	25.0%
!!!	ATTTTTTTTTGGGGGTATAT+AGG	+	chr8_1:89338680-89338699	Msa1224400:intron	25.0%
!!!	TTTTTTTTTGGGGGTATATA+GGG	+	chr8_1:89338681-89338700	Msa1224400:intron	25.0%
!	AGAAAACACAAATCCTTTAT+AGG	+	chr8_1:89338846-89338865	Msa1224400:intron	25.0%
!	TCATTAGTGAAAACCTATAA+AGG	-	chr8_1:89338862-89338881	None:intergenic	25.0%
!	TGATAAAATTAATGCAGCAA+AGG	+	chr8_1:89339480-89339499	Msa1224400:CDS	25.0%
!	GCAAAACAATTGAAAAGTTA+TGG	-	chr8_1:89339812-89339831	Msa1224410:three_prime_UTR	25.0%
!	AAAAAATAAGAAAAGGAGAC+AGG	-	chr8_1:89339928-89339947	Msa1224410:three_prime_UTR	25.0%
	ATATGCCGACAAAATTTGAT+TGG	-	chr8_1:89338310-89338329	None:intergenic	30.0%
	TTATGTCTTGTAAGTACTTC+TGG	+	chr8_1:89338375-89338394	Msa1224400:intron	30.0%
!	TTTTCACAATACCAATTGTG+TGG	+	chr8_1:89338412-89338431	Msa1224400:intron	30.0%
	TGATTCCTGATAGTACATAA+AGG	+	chr8_1:89338642-89338661	Msa1224400:intron	30.0%
	GATTCCTGATAGTACATAAA+GGG	+	chr8_1:89338643-89338662	Msa1224400:intron	30.0%
!!!	TTTTTTTTGGGGGTATATAG+GGG	+	chr8_1:89338682-89338701	Msa1224400:intron	30.0%
	AAGAAAGGAAACTAACTAAG+TGG	-	chr8_1:89338795-89338814	None:intergenic	30.0%
!!!	GTTTCCTTTCTTATTTTCCT+TGG	+	chr8_1:89338803-89338822	Msa1224400:intron	30.0%
	AATTGTATTACATTCGACCA+AGG	-	chr8_1:89338823-89338842	None:intergenic	30.0%
!	ATCAAGTTTTCAGACATTTC+TGG	+	chr8_1:89338912-89338931	Msa1224400:intron	30.0%
	TAAGTCATGTGAATTTGAGA+AGG	+	chr8_1:89339332-89339351	Msa1224400:CDS	30.0%
	CATAATCACATTATCATCCT+TGG	-	chr8_1:89339380-89339399	Msa1224410:three_prime_UTR	30.0%
!	TGATTATGCATTTGAGATGT+TGG	+	chr8_1:89339392-89339411	Msa1224400:CDS	30.0%
	TTCACGTGTAGATTAAGTAT+TGG	+	chr8_1:89339584-89339603	Msa1224400:intron	30.0%
!	TTTTCAAAAGGACTTAAACC+TGG	+	chr8_1:89339641-89339660	Msa1224400:CDS	30.0%
	TGAAGCTGACCAAAAAATTT+AGG	+	chr8_1:89339782-89339801	Msa1224400:CDS	30.0%
!!!	TATGGCTTGCCTAAATTTTT+TGG	-	chr8_1:89339794-89339813	Msa1224410:three_prime_UTR	30.0%
	ACCAACAAAACTTCTCTTTA+TGG	-	chr8_1:89339853-89339872	Msa1224410:three_prime_UTR	30.0%
	GTACGAAAATAGAAAAGCAA+GGG	-	chr8_1:89339997-89340016	Msa1224410:three_prime_UTR	30.0%
	TGTACGAAAATAGAAAAGCA+AGG	-	chr8_1:89339998-89340017	Msa1224410:three_prime_UTR	30.0%
	ATATGCCGACAAAATTTGAT+TGG	-	chr8_1:89338310-89338329	None:intergenic	30.0%
	TTATGTCTTGTAAGTACTTC+TGG	+	chr8_1:89338375-89338394	Msa1224400:intron	30.0%
!	TTTTCACAATACCAATTGTG+TGG	+	chr8_1:89338412-89338431	Msa1224400:intron	30.0%
	TGATTCCTGATAGTACATAA+AGG	+	chr8_1:89338642-89338661	Msa1224400:intron	30.0%
	GATTCCTGATAGTACATAAA+GGG	+	chr8_1:89338643-89338662	Msa1224400:intron	30.0%
!!!	TTTTTTTTGGGGGTATATAG+GGG	+	chr8_1:89338682-89338701	Msa1224400:intron	30.0%
	AAGAAAGGAAACTAACTAAG+TGG	-	chr8_1:89338795-89338814	None:intergenic	30.0%
!!!	GTTTCCTTTCTTATTTTCCT+TGG	+	chr8_1:89338803-89338822	Msa1224400:intron	30.0%
	AATTGTATTACATTCGACCA+AGG	-	chr8_1:89338823-89338842	None:intergenic	30.0%
!	ATCAAGTTTTCAGACATTTC+TGG	+	chr8_1:89338912-89338931	Msa1224400:intron	30.0%
	TAAGTCATGTGAATTTGAGA+AGG	+	chr8_1:89339332-89339351	Msa1224400:CDS	30.0%
	CATAATCACATTATCATCCT+TGG	-	chr8_1:89339380-89339399	Msa1224410:three_prime_UTR	30.0%
!	TGATTATGCATTTGAGATGT+TGG	+	chr8_1:89339392-89339411	Msa1224400:CDS	30.0%
	TTCACGTGTAGATTAAGTAT+TGG	+	chr8_1:89339584-89339603	Msa1224400:intron	30.0%
!	TTTTCAAAAGGACTTAAACC+TGG	+	chr8_1:89339641-89339660	Msa1224400:CDS	30.0%
	TGAAGCTGACCAAAAAATTT+AGG	+	chr8_1:89339782-89339801	Msa1224400:CDS	30.0%
!!!	TATGGCTTGCCTAAATTTTT+TGG	-	chr8_1:89339794-89339813	Msa1224410:three_prime_UTR	30.0%
	ACCAACAAAACTTCTCTTTA+TGG	-	chr8_1:89339853-89339872	Msa1224410:three_prime_UTR	30.0%
	GTACGAAAATAGAAAAGCAA+GGG	-	chr8_1:89339997-89340016	Msa1224410:three_prime_UTR	30.0%
	TGTACGAAAATAGAAAAGCA+AGG	-	chr8_1:89339998-89340017	Msa1224410:three_prime_UTR	30.0%
	GAAAAACATAAGCACTCACT+GGG	-	chr8_1:89338345-89338364	None:intergenic	35.0%
!!!	CTTGTAAGTACTTCTGGTTT+TGG	+	chr8_1:89338381-89338400	Msa1224400:intron	35.0%
	AAGATCCGAAGGCTTATATT+CGG	+	chr8_1:89338514-89338533	Msa1224400:intron	35.0%
	AAAGTCCGAATATAAGCCTT+CGG	-	chr8_1:89338522-89338541	None:intergenic	35.0%
	CTGCAACTGCAATGAATTAT+TGG	+	chr8_1:89338607-89338626	Msa1224400:intron	35.0%
	TATCAGGAATCAGATTCCTT+GGG	-	chr8_1:89338634-89338653	None:intergenic	35.0%
	ACATCCCTTTATGTACTATC+AGG	-	chr8_1:89338650-89338669	None:intergenic	35.0%
	GGGTATTATACATACATAGC+AGG	+	chr8_1:89338702-89338721	Msa1224400:intron	35.0%
	ATTATACATACATAGCAGGC+AGG	+	chr8_1:89338706-89338725	Msa1224400:intron	35.0%
	TCGACCAAGGAAAATAAGAA+AGG	-	chr8_1:89338810-89338829	None:intergenic	35.0%
!	CTCCTTTTCCTCAATCAAAA+CGG	-	chr8_1:89339187-89339206	None:intergenic	35.0%
	ATCCAAGTGCTAATTCAAAG+TGG	-	chr8_1:89339267-89339286	None:intergenic	35.0%
!	GTCAACTGAAAGATGGTTTT+TGG	+	chr8_1:89339354-89339373	Msa1224400:CDS	35.0%
	TTTATCAGTGTTACACAGTC+TGG	+	chr8_1:89339552-89339571	Msa1224400:intron	35.0%
	TTATCAGTGTTACACAGTCT+GGG	+	chr8_1:89339553-89339572	Msa1224400:intron	35.0%
!!	TGAAGCAGCTGATTTTCAAA+AGG	+	chr8_1:89339629-89339648	Msa1224400:CDS	35.0%
	TTTCTCATCAGGAATTTCGA+TGG	-	chr8_1:89339715-89339734	Msa1224410:three_prime_UTR	35.0%
!!!	AATTTTTTGGTCAGCTTCAG+AGG	-	chr8_1:89339781-89339800	Msa1224410:three_prime_UTR	35.0%
!!	GCCATAAAGAGAAGTTTTGT+TGG	+	chr8_1:89339849-89339868	Msa1224400:three_prime_UTR	35.0%
!!!	AGCAAGGGAATATTTTGATC+AGG	-	chr8_1:89339982-89340001	Msa1224410:three_prime_UTR	35.0%
	GAAAAACATAAGCACTCACT+GGG	-	chr8_1:89338345-89338364	None:intergenic	35.0%
!!!	CTTGTAAGTACTTCTGGTTT+TGG	+	chr8_1:89338381-89338400	Msa1224400:intron	35.0%
	AAGATCCGAAGGCTTATATT+CGG	+	chr8_1:89338514-89338533	Msa1224400:intron	35.0%
	AAAGTCCGAATATAAGCCTT+CGG	-	chr8_1:89338522-89338541	None:intergenic	35.0%
	CTGCAACTGCAATGAATTAT+TGG	+	chr8_1:89338607-89338626	Msa1224400:intron	35.0%
	TATCAGGAATCAGATTCCTT+GGG	-	chr8_1:89338634-89338653	None:intergenic	35.0%
	ACATCCCTTTATGTACTATC+AGG	-	chr8_1:89338650-89338669	None:intergenic	35.0%
	GGGTATTATACATACATAGC+AGG	+	chr8_1:89338702-89338721	Msa1224400:intron	35.0%
	ATTATACATACATAGCAGGC+AGG	+	chr8_1:89338706-89338725	Msa1224400:intron	35.0%
	TCGACCAAGGAAAATAAGAA+AGG	-	chr8_1:89338810-89338829	None:intergenic	35.0%
!	CTCCTTTTCCTCAATCAAAA+CGG	-	chr8_1:89339187-89339206	None:intergenic	35.0%
	ATCCAAGTGCTAATTCAAAG+TGG	-	chr8_1:89339267-89339286	None:intergenic	35.0%
!	GTCAACTGAAAGATGGTTTT+TGG	+	chr8_1:89339354-89339373	Msa1224400:CDS	35.0%
	TTTATCAGTGTTACACAGTC+TGG	+	chr8_1:89339552-89339571	Msa1224400:intron	35.0%
	TTATCAGTGTTACACAGTCT+GGG	+	chr8_1:89339553-89339572	Msa1224400:intron	35.0%
!!	TGAAGCAGCTGATTTTCAAA+AGG	+	chr8_1:89339629-89339648	Msa1224400:CDS	35.0%
	TTTCTCATCAGGAATTTCGA+TGG	-	chr8_1:89339715-89339734	Msa1224410:three_prime_UTR	35.0%
!!!	AATTTTTTGGTCAGCTTCAG+AGG	-	chr8_1:89339781-89339800	Msa1224410:three_prime_UTR	35.0%
!!	GCCATAAAGAGAAGTTTTGT+TGG	+	chr8_1:89339849-89339868	Msa1224400:three_prime_UTR	35.0%
!!!	AGCAAGGGAATATTTTGATC+AGG	-	chr8_1:89339982-89340001	Msa1224410:three_prime_UTR	35.0%
	GGAAAAACATAAGCACTCAC+TGG	-	chr8_1:89338346-89338365	None:intergenic	40.0%
	TCACAATACCAATTGTGTGG+TGG	+	chr8_1:89338415-89338434	Msa1224400:intron	40.0%
	ATCAGGAATCAGATTCCTTG+GGG	-	chr8_1:89338633-89338652	None:intergenic	40.0%
	CTATCAGGAATCAGATTCCT+TGG	-	chr8_1:89338635-89338654	None:intergenic	40.0%
!!!	TCTATGCACCGTTTTGATTG+AGG	+	chr8_1:89339176-89339195	Msa1224400:five_prime_UTR	40.0%
!!!	CACCGTTTTGATTGAGGAAA+AGG	+	chr8_1:89339182-89339201	Msa1224400:five_prime_UTR	40.0%
!!!	CGTTTTGATTGAGGAAAAGG+AGG	+	chr8_1:89339185-89339204	Msa1224400:five_prime_UTR	40.0%
!!	TGCCACTTTGAATTAGCACT+TGG	+	chr8_1:89339262-89339281	Msa1224400:five_prime_UTR	40.0%
	TGAGAAGGTCAACTGAAAGA+TGG	+	chr8_1:89339347-89339366	Msa1224400:CDS	40.0%
!!!	TGAAAGATGGTTTTTGGCCA+AGG	+	chr8_1:89339360-89339379	Msa1224400:CDS	40.0%
	CTGATTACTCGGTCTAGTAT+CGG	-	chr8_1:89339445-89339464	Msa1224410:three_prime_UTR	40.0%
	TCTTGACCAGTCTGATTACT+CGG	-	chr8_1:89339456-89339475	Msa1224410:three_prime_UTR	40.0%
	GGATAGGGTTGAGCTTATTA+TGG	+	chr8_1:89339671-89339690	Msa1224400:CDS	40.0%
	AGCTTATCTGCTTTCTCATC+AGG	-	chr8_1:89339726-89339745	Msa1224410:three_prime_UTR	40.0%
	GGCTATGTACTTTGCAACAT+CGG	-	chr8_1:89339760-89339779	Msa1224410:three_prime_UTR	40.0%
!!	GTTTTGTTGGTTGAGCTGTT+TGG	+	chr8_1:89339862-89339881	Msa1224400:three_prime_UTR	40.0%
!	TCAGGTGTCATGCTTACATT+GGG	-	chr8_1:89339964-89339983	Msa1224410:three_prime_UTR	40.0%
!	ATCAGGTGTCATGCTTACAT+TGG	-	chr8_1:89339965-89339984	Msa1224410:three_prime_UTR	40.0%
	GGAAAAACATAAGCACTCAC+TGG	-	chr8_1:89338346-89338365	None:intergenic	40.0%
	TCACAATACCAATTGTGTGG+TGG	+	chr8_1:89338415-89338434	Msa1224400:intron	40.0%
	ATCAGGAATCAGATTCCTTG+GGG	-	chr8_1:89338633-89338652	None:intergenic	40.0%
	CTATCAGGAATCAGATTCCT+TGG	-	chr8_1:89338635-89338654	None:intergenic	40.0%
!!!	TCTATGCACCGTTTTGATTG+AGG	+	chr8_1:89339176-89339195	Msa1224400:five_prime_UTR	40.0%
!!!	CACCGTTTTGATTGAGGAAA+AGG	+	chr8_1:89339182-89339201	Msa1224400:five_prime_UTR	40.0%
!!!	CGTTTTGATTGAGGAAAAGG+AGG	+	chr8_1:89339185-89339204	Msa1224400:five_prime_UTR	40.0%
!!	TGCCACTTTGAATTAGCACT+TGG	+	chr8_1:89339262-89339281	Msa1224400:five_prime_UTR	40.0%
	TGAGAAGGTCAACTGAAAGA+TGG	+	chr8_1:89339347-89339366	Msa1224400:CDS	40.0%
!!!	TGAAAGATGGTTTTTGGCCA+AGG	+	chr8_1:89339360-89339379	Msa1224400:CDS	40.0%
	CTGATTACTCGGTCTAGTAT+CGG	-	chr8_1:89339445-89339464	Msa1224410:three_prime_UTR	40.0%
	TCTTGACCAGTCTGATTACT+CGG	-	chr8_1:89339456-89339475	Msa1224410:three_prime_UTR	40.0%
	GGATAGGGTTGAGCTTATTA+TGG	+	chr8_1:89339671-89339690	Msa1224400:CDS	40.0%
	AGCTTATCTGCTTTCTCATC+AGG	-	chr8_1:89339726-89339745	Msa1224410:three_prime_UTR	40.0%
	GGCTATGTACTTTGCAACAT+CGG	-	chr8_1:89339760-89339779	Msa1224410:three_prime_UTR	40.0%
!!	GTTTTGTTGGTTGAGCTGTT+TGG	+	chr8_1:89339862-89339881	Msa1224400:three_prime_UTR	40.0%
!	TCAGGTGTCATGCTTACATT+GGG	-	chr8_1:89339964-89339983	Msa1224410:three_prime_UTR	40.0%
!	ATCAGGTGTCATGCTTACAT+TGG	-	chr8_1:89339965-89339984	Msa1224410:three_prime_UTR	40.0%
	AGCCTTCGGATCTTCAATCA+GGG	-	chr8_1:89338508-89338527	None:intergenic	45.0%
	AAGCCTTCGGATCTTCAATC+AGG	-	chr8_1:89338509-89338528	None:intergenic	45.0%
	GCAATGAATTATTGGCCCCA+AGG	+	chr8_1:89338615-89338634	Msa1224400:intron	45.0%
	ACTAGACCGAGTAATCAGAC+TGG	+	chr8_1:89339447-89339466	Msa1224400:CDS	45.0%
	TAAACCTGGACAGCTTGGAT+AGG	+	chr8_1:89339655-89339674	Msa1224400:CDS	45.0%
	AAACCTGGACAGCTTGGATA+GGG	+	chr8_1:89339656-89339675	Msa1224400:CDS	45.0%
	TGTCATGCTTACATTGGGGT+GGG	-	chr8_1:89339959-89339978	Msa1224410:three_prime_UTR	45.0%
!	CAGGTGTCATGCTTACATTG+GGG	-	chr8_1:89339963-89339982	Msa1224410:three_prime_UTR	45.0%
	AGCCTTCGGATCTTCAATCA+GGG	-	chr8_1:89338508-89338527	None:intergenic	45.0%
	AAGCCTTCGGATCTTCAATC+AGG	-	chr8_1:89338509-89338528	None:intergenic	45.0%
	GCAATGAATTATTGGCCCCA+AGG	+	chr8_1:89338615-89338634	Msa1224400:intron	45.0%
	ACTAGACCGAGTAATCAGAC+TGG	+	chr8_1:89339447-89339466	Msa1224400:CDS	45.0%
	TAAACCTGGACAGCTTGGAT+AGG	+	chr8_1:89339655-89339674	Msa1224400:CDS	45.0%
	AAACCTGGACAGCTTGGATA+GGG	+	chr8_1:89339656-89339675	Msa1224400:CDS	45.0%
	TGTCATGCTTACATTGGGGT+GGG	-	chr8_1:89339959-89339978	Msa1224410:three_prime_UTR	45.0%
!	CAGGTGTCATGCTTACATTG+GGG	-	chr8_1:89339963-89339982	Msa1224410:three_prime_UTR	45.0%
	CACTCACTGGGAGATTCGTT+TGG	-	chr8_1:89338333-89338352	None:intergenic	50.0%
	TCTGCGTACCACCACACAAT+TGG	-	chr8_1:89338426-89338445	None:intergenic	50.0%
	TCCCCTGATTGAAGATCCGA+AGG	+	chr8_1:89338503-89338522	Msa1224400:intron	50.0%
	GCCTTCGGATCTTCAATCAG+GGG	-	chr8_1:89338507-89338526	None:intergenic	50.0%
!	AGATGTTGGTGCTCTTCAGC+AGG	+	chr8_1:89339406-89339425	Msa1224400:CDS	50.0%
!	GTCTAGTATCGGATCAGAGC+TGG	-	chr8_1:89339434-89339453	Msa1224410:three_prime_UTR	50.0%
	GGACTTAAACCTGGACAGCT+TGG	+	chr8_1:89339650-89339669	Msa1224400:CDS	50.0%
	GTCATGCTTACATTGGGGTG+GGG	-	chr8_1:89339958-89339977	Msa1224410:three_prime_UTR	50.0%
	GTGTCATGCTTACATTGGGG+TGG	-	chr8_1:89339960-89339979	Msa1224410:three_prime_UTR	50.0%
	CACTCACTGGGAGATTCGTT+TGG	-	chr8_1:89338333-89338352	None:intergenic	50.0%
	TCTGCGTACCACCACACAAT+TGG	-	chr8_1:89338426-89338445	None:intergenic	50.0%
	TCCCCTGATTGAAGATCCGA+AGG	+	chr8_1:89338503-89338522	Msa1224400:intron	50.0%
	GCCTTCGGATCTTCAATCAG+GGG	-	chr8_1:89338507-89338526	None:intergenic	50.0%
!	AGATGTTGGTGCTCTTCAGC+AGG	+	chr8_1:89339406-89339425	Msa1224400:CDS	50.0%
!	GTCTAGTATCGGATCAGAGC+TGG	-	chr8_1:89339434-89339453	Msa1224410:three_prime_UTR	50.0%
	GGACTTAAACCTGGACAGCT+TGG	+	chr8_1:89339650-89339669	Msa1224400:CDS	50.0%
	GTCATGCTTACATTGGGGTG+GGG	-	chr8_1:89339958-89339977	Msa1224410:three_prime_UTR	50.0%
	GTGTCATGCTTACATTGGGG+TGG	-	chr8_1:89339960-89339979	Msa1224410:three_prime_UTR	50.0%
	CAACCCTATCCAAGCTGTCC+AGG	-	chr8_1:89339662-89339681	Msa1224410:three_prime_UTR	55.0%
	CAACCCTATCCAAGCTGTCC+AGG	-	chr8_1:89339662-89339681	Msa1224410:three_prime_UTR	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	89338275	89340039	89338275	ID=Msa1224400;Name=Msa1224400
chr8_1	mRNA	89338275	89340039	89338275	ID=Msa1224400-mRNA-1;Parent=Msa1224400;Name=Msa1224400-mRNA-1;_AED=0.01;_eAED=0.01;_QI=204\|1\|1\|1\|0.5\|0.33\|3\|236\|124
chr8_1	exon	89338275	89338345	89338275	ID=Msa1224400-mRNA-1:exon:32401;Parent=Msa1224400-mRNA-1
chr8_1	exon	89339174	89339501	89339174	ID=Msa1224400-mRNA-1:exon:32402;Parent=Msa1224400-mRNA-1
chr8_1	exon	89339624	89340039	89339624	ID=Msa1224400-mRNA-1:exon:32403;Parent=Msa1224400-mRNA-1
chr8_1	five_prime_UTR	89338275	89338345	89338275	ID=Msa1224400-mRNA-1:five_prime_utr;Parent=Msa1224400-mRNA-1
chr8_1	five_prime_UTR	89339174	89339306	89339174	ID=Msa1224400-mRNA-1:five_prime_utr;Parent=Msa1224400-mRNA-1
chr8_1	CDS	89339307	89339501	89339307	ID=Msa1224400-mRNA-1:cds;Parent=Msa1224400-mRNA-1
chr8_1	CDS	89339624	89339803	89339624	ID=Msa1224400-mRNA-1:cds;Parent=Msa1224400-mRNA-1
chr8_1	three_prime_UTR	89339804	89340039	89339804	ID=Msa1224400-mRNA-1:three_prime_utr;Parent=Msa1224400-mRNA-1

Gene Sequence	>Msa1224400 ATGCAAAGCATAAAGAAATCTATACTAAGTCATGTGAATTTGAGAAGGTCAACTGAAAGATGGTTTTTGGCCAAGGATGATAATGTGATTATGCATTTGAGATGTTGGTGCTCTTCAGCAGGTGCCAGCTCTGATCCGATACTAGACCGAGTAATCAGACTGGTCAAGAAATATGATAAAATTAATGCAGCAAAGGTTACTGAAGCAGCTGATTTTCAAAAGGACTTAAACCTGGACAGCTTGGATAGGGTTGAGCTTATTATGGCTCTTGAAGAAGAATTTTCCATCGAAATTCCTGATGAGAAAGCAGATAAGCTTACTTGCTGTACCGATGTTGCAAAGTACATAGCCTCTGAAGCTGACCAAAAAATTTAG
Protein sequence	>Msa1224400 MQSIKKSILSHVNLRRSTERWFLAKDDNVIMHLRCWCSSAGASSDPILDRVIRLVKKYDKINAAKVTEAADFQKDLNLDSLDRVELIMALEEEFSIEIPDEKADKLTCCTDVAKYIASEADQKI*