AlfalfaGEDB | Msa1389120

M. sativa cultivar zhongmu-4 / Msa1389120

KEGG: K03955
GO: GO:0000035 GO:0000036 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009245 GO:0009247 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016053 GO:0019637 GO:0019752 GO:0019842 GO:0031177 GO:0032787 GO:0033218 GO:0036094 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0044620 GO:0046394 GO:0046467 GO:0046493 GO:0048037 GO:0051192 GO:0071704 GO:0072330 GO:0072341 GO:0090407 GO:0140104 GO:1901135 GO:1901137 GO:1901269 GO:1901271 GO:1901576 GO:1903509
PFAM: PF00550.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389120	PNY14930.1	95.161	124	6	0	1	124	1	124	1.61e-79	239

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389120	sp\|Q9FGJ4\|ACPM3_ARATH	56.693	127	51	1	1	123	1	127	1.07e-41	137

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389120	A0A2K3PI09	95.161	124	6	0	1	124	1	124	7.68e-80	239

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008330	Msa1389120	0.806066	9.850275e-50	-8.615850e-47
Msa0036150	Msa1389120	0.831182	1.929367e-55	-8.615850e-47
Msa0040190	Msa1389120	0.819361	1.209096e-52	-8.615850e-47
Msa0075850	Msa1389120	0.805647	1.206820e-49	-8.615850e-47
Msa1355260	Msa1389120	0.843350	1.479512e-58	-8.615850e-47
Msa1373950	Msa1389120	0.827864	1.235880e-54	-8.615850e-47
Msa1387090	Msa1389120	0.816886	4.387950e-52	-8.615850e-47
Msa1389120	Msa1390410	0.829034	6.451672e-55	-8.615850e-47
Msa1389120	Msa1397540	0.801610	8.300250e-49	-8.615850e-47
Msa1389120	Msa1399430	0.800108	1.682589e-48	-8.615850e-47
Msa1389120	Msa1409520	0.806044	9.959794e-50	-8.615850e-47
Msa0619330	Msa1389120	0.818280	2.128656e-52	-8.615850e-47
Msa0621890	Msa1389120	0.805307	1.422249e-49	-8.615850e-47
Msa0694690	Msa1389120	-0.800285	1.548288e-48	-8.615850e-47
Msa0701770	Msa1389120	0.802205	6.265069e-49	-8.615850e-47
Msa0725480	Msa1389120	-0.803336	3.660584e-49	-8.615850e-47
Msa0739570	Msa1389120	0.833090	6.511476e-56	-8.615850e-47
Msa0749550	Msa1389120	0.810599	1.064032e-50	-8.615850e-47
Msa0749610	Msa1389120	0.803184	3.934047e-49	-8.615850e-47
Msa0756480	Msa1389120	0.821789	3.348900e-53	-8.615850e-47
Msa0766100	Msa1389120	-0.808962	2.393717e-50	-8.615850e-47
Msa0782330	Msa1389120	0.814317	1.638156e-51	-8.615850e-47
Msa0792510	Msa1389120	0.825280	5.109167e-54	-8.615850e-47
Msa0815380	Msa1389120	0.802660	5.048467e-49	-8.615850e-47
Msa0835510	Msa1389120	0.827769	1.303179e-54	-8.615850e-47
Msa0837990	Msa1389120	0.800088	1.697837e-48	-8.615850e-47
Msa0134550	Msa1389120	0.818630	1.773446e-52	-8.615850e-47
Msa0141110	Msa1389120	-0.800183	1.624088e-48	-8.615850e-47
Msa0164660	Msa1389120	0.801475	8.850169e-49	-8.615850e-47
Msa0168390	Msa1389120	0.809496	1.838286e-50	-8.615850e-47
Msa0189050	Msa1389120	0.823986	1.030791e-53	-8.615850e-47
Msa0211010	Msa1389120	0.801616	8.276882e-49	-8.615850e-47
Msa0231310	Msa1389120	0.824649	7.197724e-54	-8.615850e-47
Msa1238480	Msa1389120	0.803064	4.166345e-49	-8.615850e-47
Msa1244830	Msa1389120	0.804560	2.037517e-49	-8.615850e-47
Msa1254770	Msa1389120	0.814489	1.500959e-51	-8.615850e-47
Msa1254800	Msa1389120	0.813321	2.715739e-51	-8.615850e-47
Msa1287160	Msa1389120	0.809282	2.044113e-50	-8.615850e-47
Msa1287340	Msa1389120	0.805004	1.645325e-49	-8.615850e-47
Msa1329990	Msa1389120	0.803348	3.638047e-49	-8.615850e-47
Msa1332450	Msa1389120	0.993105	1.743915e-197	-8.615850e-47
Msa1337190	Msa1389120	0.951289	2.792247e-109	-8.615850e-47
Msa0365360	Msa1389120	0.828664	7.929489e-55	-8.615850e-47
Msa0371610	Msa1389120	0.822795	1.956858e-53	-8.615850e-47
Msa0374860	Msa1389120	-0.802154	6.418070e-49	-8.615850e-47
Msa0413740	Msa1389120	0.814485	1.504343e-51	-8.615850e-47
Msa0418000	Msa1389120	0.838653	2.530764e-57	-8.615850e-47
Msa0431710	Msa1389120	0.806494	8.004080e-50	-8.615850e-47
Msa0436900	Msa1389120	0.808130	3.602210e-50	-8.615850e-47
Msa0457370	Msa1389120	0.807525	4.843336e-50	-8.615850e-47
Msa0458790	Msa1389120	0.803344	3.645390e-49	-8.615850e-47
Msa0466100	Msa1389120	0.805156	1.529093e-49	-8.615850e-47
Msa0470350	Msa1389120	-0.801586	8.394862e-49	-8.615850e-47
Msa0475830	Msa1389120	0.817850	2.662716e-52	-8.615850e-47
Msa1009000	Msa1389120	0.815149	1.072074e-51	-8.615850e-47
Msa1018720	Msa1389120	0.807050	6.108950e-50	-8.615850e-47
Msa1064420	Msa1389120	0.809749	1.622519e-50	-8.615850e-47
Msa1069300	Msa1389120	0.802606	5.180716e-49	-8.615850e-47
Msa1079610	Msa1389120	0.810375	1.189045e-50	-8.615850e-47
Msa1081600	Msa1389120	0.855495	6.072193e-62	-8.615850e-47
Msa0257930	Msa1389120	-0.812308	4.526092e-51	-8.615850e-47
Msa0286790	Msa1389120	0.820669	6.069569e-53	-8.615850e-47
Msa0304990	Msa1389120	0.827812	1.272303e-54	-8.615850e-47
Msa0306130	Msa1389120	0.839127	1.908162e-57	-8.615850e-47
Msa0309160	Msa1389120	0.827505	1.507759e-54	-8.615850e-47
Msa0320540	Msa1389120	0.804529	2.068250e-49	-8.615850e-47
Msa1125040	Msa1389120	0.806504	7.967947e-50	-8.615850e-47
Msa1166370	Msa1389120	0.842766	2.116616e-58	-8.615850e-47
Msa1201990	Msa1389120	0.805750	1.147928e-49	-8.615850e-47
Msa1208070	Msa1389120	0.801448	8.963173e-49	-8.615850e-47
Msa1211370	Msa1389120	0.804117	2.519778e-49	-8.615850e-47
Msa1220710	Msa1389120	0.814183	1.753818e-51	-8.615850e-47
Msa1221200	Msa1389120	-0.809616	1.732348e-50	-8.615850e-47
Msa1224400	Msa1389120	0.996548	5.901795e-229	-8.615850e-47
Msa0498920	Msa1389120	0.808004	3.831848e-50	-8.615850e-47
Msa0504630	Msa1389120	0.816355	5.772623e-52	-8.615850e-47
Msa0505390	Msa1389120	0.808392	3.168568e-50	-8.615850e-47
Msa0535020	Msa1389120	0.810699	1.012491e-50	-8.615850e-47
Msa0535050	Msa1389120	0.818230	2.184505e-52	-8.615850e-47
Msa0575250	Msa1389120	0.810548	1.091388e-50	-8.615850e-47
Msa0591780	Msa1389120	0.824394	8.267572e-54	-8.615850e-47
Msa0855520	Msa1389120	0.818465	1.932671e-52	-8.615850e-47
Msa0860270	Msa1389120	0.845766	3.308139e-59	-8.615850e-47
Msa0880080	Msa1389120	0.802132	6.486898e-49	-8.615850e-47
Msa0951720	Msa1389120	0.800044	1.733362e-48	-8.615850e-47
Msa0960270	Msa1389120	0.814825	1.264598e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389120	MtrunA17_Chr4g0076301	94.355	124	7	1	124	1	124	6.58e-83	238
Msa1389120	MtrunA17_Chr7g0249561	50.649	77	38	47	123	54	130	2.02e-21	82.8
Msa1389120	MtrunA17_Chr7g0249801	48.649	74	38	47	120	43	116	2.03e-21	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1389120	AT5G47630.1	56.693	127	51	1	1	123	1	127	1.09e-42	137
Msa1389120	AT5G47630.2	56.693	127	51	1	1	123	1	127	1.09e-42	137
Msa1389120	AT5G47630.3	56.693	127	51	1	1	123	1	127	1.09e-42	137
Msa1389120	AT1G65290.1	50.649	77	38	0	47	123	50	126	3.52e-21	82.4
Msa1389120	AT2G44620.1	41.000	100	46	1	17	116	29	115	6.34e-21	81.6

Find 34 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCAACAAAACTTCTCTTTA+TGG	0.154842	tig0021212:-86130	Msa1389130:three_prime_UTR
GGATAGGGTTGAGCTTATTA+TGG	0.307345	tig0021212:+85951	Msa1389120:CDS
AGTAAAAGTGAAAATAATAT+TGG	0.326289	tig0021212:-85494	None:intergenic
AGCTTATCTGCTTTCTCATC+AGG	0.361227	tig0021212:-86003	Msa1389130:three_prime_UTR
GCAAAACAATTGAAAAGTTA+TGG	0.376214	tig0021212:-86089	Msa1389130:three_prime_UTR
ATATGCCGACAAAATTTGAT+TGG	0.384777	tig0021212:-84587	None:intergenic
CACTCACTGGGAGATTCGTT+TGG	0.407167	tig0021212:-84610	None:intergenic
TCAGGTGTCATGCTTACATT+GGG	0.419498	tig0021212:-86241	Msa1389130:three_prime_UTR
ATCAGGTGTCATGCTTACAT+TGG	0.439491	tig0021212:-86242	Msa1389130:three_prime_UTR
CATAATCACATTATCATCCT+TGG	0.439969	tig0021212:-85657	Msa1389130:three_prime_UTR
GGACTTAAACCTGGACAGCT+TGG	0.459469	tig0021212:+85930	Msa1389120:CDS
TGATTATGCATTTGAGATGT+TGG	0.460487	tig0021212:+85672	Msa1389120:CDS
TGAGAAGGTCAACTGAAAGA+TGG	0.475010	tig0021212:+85627	Msa1389120:CDS
GTCTAGTATCGGATCAGAGC+TGG	0.475971	tig0021212:-85711	Msa1389130:three_prime_UTR
TGTCATGCTTACATTGGGGT+GGG	0.479726	tig0021212:-86236	Msa1389130:three_prime_UTR
AGATGTTGGTGCTCTTCAGC+AGG	0.487417	tig0021212:+85686	Msa1389120:CDS
CAACCCTATCCAAGCTGTCC+AGG	0.498453	tig0021212:-85939	Msa1389130:three_prime_UTR
TGCCACTTTGAATTAGCACT+TGG	0.508091	tig0021212:+85542	Msa1389120:five_prime_UTR
TTCAATAAAGCATAACTCAA+CGG	0.513754	tig0021212:-86312	Msa1389130:three_prime_UTR
TAAACCTGGACAGCTTGGAT+AGG	0.517739	tig0021212:+85935	Msa1389120:CDS
GTGTCATGCTTACATTGGGG+TGG	0.521344	tig0021212:-86237	Msa1389130:three_prime_UTR
TCTTGACCAGTCTGATTACT+CGG	0.521850	tig0021212:-85733	Msa1389130:three_prime_UTR
AAACCTGGACAGCTTGGATA+GGG	0.522123	tig0021212:+85936	Msa1389120:CDS
CTGATTACTCGGTCTAGTAT+CGG	0.536163	tig0021212:-85722	Msa1389130:three_prime_UTR
GTCATGCTTACATTGGGGTG+GGG	0.536900	tig0021212:-86235	Msa1389130:three_prime_UTR
GGCTATGTACTTTGCAACAT+CGG	0.551029	tig0021212:-86037	Msa1389130:three_prime_UTR
TTTCTCATCAGGAATTTCGA+TGG	0.580152	tig0021212:-85992	Msa1389130:three_prime_UTR
TGATAAAATTAATGCAGCAA+AGG	0.580568	tig0021212:+85760	Msa1389120:CDS
TGTACGAAAATAGAAAAGCA+AGG	0.585420	tig0021212:-86275	Msa1389130:three_prime_UTR
GTACGAAAATAGAAAAGCAA+GGG	0.606712	tig0021212:-86274	Msa1389130:three_prime_UTR
TAAGTCATGTGAATTTGAGA+AGG	0.613186	tig0021212:+85612	Msa1389120:CDS
ATCCAAGTGCTAATTCAAAG+TGG	0.625412	tig0021212:-85544	None:intergenic
ACTAGACCGAGTAATCAGAC+TGG	0.680991	tig0021212:+85727	Msa1389120:CDS
CAGGTGTCATGCTTACATTG+GGG	0.762397	tig0021212:-86240	Msa1389130:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGTCATTTTTTATTTTTTTT+TGG	+	tig0021212:84949-84968	Msa1389120:intron	10.0%
!!!	GTCATTTTTTATTTTTTTTT+GGG	+	tig0021212:84950-84969	Msa1389120:intron	10.0%
!!!	TCATTTTTTATTTTTTTTTG+GGG	+	tig0021212:84951-84970	Msa1389120:intron	10.0%
!!!	TGTCATTTTTTATTTTTTTT+TGG	+	tig0021212:84949-84968	Msa1389120:intron	10.0%
!!!	GTCATTTTTTATTTTTTTTT+GGG	+	tig0021212:84950-84969	Msa1389120:intron	10.0%
!!!	TCATTTTTTATTTTTTTTTG+GGG	+	tig0021212:84951-84970	Msa1389120:intron	10.0%
!!!	CATTTTTTATTTTTTTTTGG+GGG	+	tig0021212:84952-84971	Msa1389120:intron	15.0%
!!!	GATTTTGTTATTTTTAATGA+AGG	+	tig0021212:85359-85378	Msa1389120:intron	15.0%
!!	AGTAAAAGTGAAAATAATAT+TGG	-	tig0021212:85497-85516	None:intergenic	15.0%
!!	TGACATAAAAAAATAAGAAA+AGG	-	tig0021212:86215-86234	Msa1389130:three_prime_UTR	15.0%
!!!	CATTTTTTATTTTTTTTTGG+GGG	+	tig0021212:84952-84971	Msa1389120:intron	15.0%
!!!	GATTTTGTTATTTTTAATGA+AGG	+	tig0021212:85359-85378	Msa1389120:intron	15.0%
!!	AGTAAAAGTGAAAATAATAT+TGG	-	tig0021212:85497-85516	None:intergenic	15.0%
!!	TGACATAAAAAAATAAGAAA+AGG	-	tig0021212:86215-86234	Msa1389130:three_prime_UTR	15.0%
!!!	TTAATCCAATCAAATTTTGT+CGG	+	tig0021212:84582-84601	Msa1389120:five_prime_UTR	20.0%
!!	AATTCAACAATAGAAGTAAA+TGG	+	tig0021212:85013-85032	Msa1389120:intron	20.0%
!!	AAATAAAAAGTAGAAGTCAT+AGG	-	tig0021212:85310-85329	None:intergenic	20.0%
!!	TGCATGTTTATTTATATGAA+TGG	+	tig0021212:85329-85348	Msa1389120:intron	20.0%
!!!	TATTTTCTTTTGACTATAAC+TGG	-	tig0021212:85790-85809	Msa1389130:three_prime_UTR	20.0%
!!!	ATTGGTATATATTTTTGTGT+AGG	+	tig0021212:85882-85901	Msa1389120:intron	20.0%
!!!	TTAATCCAATCAAATTTTGT+CGG	+	tig0021212:84582-84601	Msa1389120:five_prime_UTR	20.0%
!!	AATTCAACAATAGAAGTAAA+TGG	+	tig0021212:85013-85032	Msa1389120:intron	20.0%
!!	AAATAAAAAGTAGAAGTCAT+AGG	-	tig0021212:85310-85329	None:intergenic	20.0%
!!	TGCATGTTTATTTATATGAA+TGG	+	tig0021212:85329-85348	Msa1389120:intron	20.0%
!!!	TATTTTCTTTTGACTATAAC+TGG	-	tig0021212:85790-85809	Msa1389130:three_prime_UTR	20.0%
!!!	ATTGGTATATATTTTTGTGT+AGG	+	tig0021212:85882-85901	Msa1389120:intron	20.0%
!	TACAAGACATAAAGAAGAAA+AGG	-	tig0021212:84647-84666	None:intergenic	25.0%
!!!	ATTTTTTTTTGGGGGTATAT+AGG	+	tig0021212:84960-84979	Msa1389120:intron	25.0%
!!!	TTTTTTTTTGGGGGTATATA+GGG	+	tig0021212:84961-84980	Msa1389120:intron	25.0%
!	AGAAAACACAAATCCTTTAT+AGG	+	tig0021212:85126-85145	Msa1389120:intron	25.0%
!	TCATTAGTGAAAACCTATAA+AGG	-	tig0021212:85142-85161	None:intergenic	25.0%
!	TGATAAAATTAATGCAGCAA+AGG	+	tig0021212:85760-85779	Msa1389120:CDS	25.0%
!	GCAAAACAATTGAAAAGTTA+TGG	-	tig0021212:86092-86111	Msa1389130:three_prime_UTR	25.0%
!	AAAAAATAAGAAAAGGAGAC+AGG	-	tig0021212:86208-86227	Msa1389130:three_prime_UTR	25.0%
!	TACAAGACATAAAGAAGAAA+AGG	-	tig0021212:84647-84666	None:intergenic	25.0%
!!!	ATTTTTTTTTGGGGGTATAT+AGG	+	tig0021212:84960-84979	Msa1389120:intron	25.0%
!!!	TTTTTTTTTGGGGGTATATA+GGG	+	tig0021212:84961-84980	Msa1389120:intron	25.0%
!	AGAAAACACAAATCCTTTAT+AGG	+	tig0021212:85126-85145	Msa1389120:intron	25.0%
!	TCATTAGTGAAAACCTATAA+AGG	-	tig0021212:85142-85161	None:intergenic	25.0%
!	TGATAAAATTAATGCAGCAA+AGG	+	tig0021212:85760-85779	Msa1389120:CDS	25.0%
!	GCAAAACAATTGAAAAGTTA+TGG	-	tig0021212:86092-86111	Msa1389130:three_prime_UTR	25.0%
!	AAAAAATAAGAAAAGGAGAC+AGG	-	tig0021212:86208-86227	Msa1389130:three_prime_UTR	25.0%
	ATATGCCGACAAAATTTGAT+TGG	-	tig0021212:84590-84609	None:intergenic	30.0%
	TTATGTCTTGTAAGTACTTC+TGG	+	tig0021212:84655-84674	Msa1389120:intron	30.0%
!	TTTTCACAATACCAATTGTG+TGG	+	tig0021212:84692-84711	Msa1389120:intron	30.0%
	TGATTCCTGATAGTACATAA+AGG	+	tig0021212:84922-84941	Msa1389120:intron	30.0%
	GATTCCTGATAGTACATAAA+GGG	+	tig0021212:84923-84942	Msa1389120:intron	30.0%
!!!	TTTTTTTTGGGGGTATATAG+GGG	+	tig0021212:84962-84981	Msa1389120:intron	30.0%
	AAGAAAGGAAACTAACTAAG+TGG	-	tig0021212:85075-85094	None:intergenic	30.0%
!!!	GTTTCCTTTCTTATTTTCCT+TGG	+	tig0021212:85083-85102	Msa1389120:intron	30.0%
	AATTGTATTACATTCGACCA+AGG	-	tig0021212:85103-85122	None:intergenic	30.0%
!	ATCAAGTTTTCAGACATTTC+TGG	+	tig0021212:85192-85211	Msa1389120:intron	30.0%
	TAAGTCATGTGAATTTGAGA+AGG	+	tig0021212:85612-85631	Msa1389120:CDS	30.0%
	CATAATCACATTATCATCCT+TGG	-	tig0021212:85660-85679	Msa1389130:three_prime_UTR	30.0%
!	TGATTATGCATTTGAGATGT+TGG	+	tig0021212:85672-85691	Msa1389120:CDS	30.0%
	TTCACGTGTAGATTAAGTAT+TGG	+	tig0021212:85864-85883	Msa1389120:intron	30.0%
!	TTTTCAAAAGGACTTAAACC+TGG	+	tig0021212:85921-85940	Msa1389120:CDS	30.0%
	TGAAGCTGACCAAAAAATTT+AGG	+	tig0021212:86062-86081	Msa1389120:CDS	30.0%
!!!	TATGGCTTGCCTAAATTTTT+TGG	-	tig0021212:86074-86093	Msa1389130:three_prime_UTR	30.0%
	ACCAACAAAACTTCTCTTTA+TGG	-	tig0021212:86133-86152	Msa1389130:three_prime_UTR	30.0%
	GTACGAAAATAGAAAAGCAA+GGG	-	tig0021212:86277-86296	Msa1389130:three_prime_UTR	30.0%
	TGTACGAAAATAGAAAAGCA+AGG	-	tig0021212:86278-86297	Msa1389130:three_prime_UTR	30.0%
	ATATGCCGACAAAATTTGAT+TGG	-	tig0021212:84590-84609	None:intergenic	30.0%
	TTATGTCTTGTAAGTACTTC+TGG	+	tig0021212:84655-84674	Msa1389120:intron	30.0%
!	TTTTCACAATACCAATTGTG+TGG	+	tig0021212:84692-84711	Msa1389120:intron	30.0%
	TGATTCCTGATAGTACATAA+AGG	+	tig0021212:84922-84941	Msa1389120:intron	30.0%
	GATTCCTGATAGTACATAAA+GGG	+	tig0021212:84923-84942	Msa1389120:intron	30.0%
!!!	TTTTTTTTGGGGGTATATAG+GGG	+	tig0021212:84962-84981	Msa1389120:intron	30.0%
	AAGAAAGGAAACTAACTAAG+TGG	-	tig0021212:85075-85094	None:intergenic	30.0%
!!!	GTTTCCTTTCTTATTTTCCT+TGG	+	tig0021212:85083-85102	Msa1389120:intron	30.0%
	AATTGTATTACATTCGACCA+AGG	-	tig0021212:85103-85122	None:intergenic	30.0%
!	ATCAAGTTTTCAGACATTTC+TGG	+	tig0021212:85192-85211	Msa1389120:intron	30.0%
	TAAGTCATGTGAATTTGAGA+AGG	+	tig0021212:85612-85631	Msa1389120:CDS	30.0%
	CATAATCACATTATCATCCT+TGG	-	tig0021212:85660-85679	Msa1389130:three_prime_UTR	30.0%
!	TGATTATGCATTTGAGATGT+TGG	+	tig0021212:85672-85691	Msa1389120:CDS	30.0%
	TTCACGTGTAGATTAAGTAT+TGG	+	tig0021212:85864-85883	Msa1389120:intron	30.0%
!	TTTTCAAAAGGACTTAAACC+TGG	+	tig0021212:85921-85940	Msa1389120:CDS	30.0%
	TGAAGCTGACCAAAAAATTT+AGG	+	tig0021212:86062-86081	Msa1389120:CDS	30.0%
!!!	TATGGCTTGCCTAAATTTTT+TGG	-	tig0021212:86074-86093	Msa1389130:three_prime_UTR	30.0%
	ACCAACAAAACTTCTCTTTA+TGG	-	tig0021212:86133-86152	Msa1389130:three_prime_UTR	30.0%
	GTACGAAAATAGAAAAGCAA+GGG	-	tig0021212:86277-86296	Msa1389130:three_prime_UTR	30.0%
	TGTACGAAAATAGAAAAGCA+AGG	-	tig0021212:86278-86297	Msa1389130:three_prime_UTR	30.0%
	GAAAAACATAAGCACTCACT+GGG	-	tig0021212:84625-84644	None:intergenic	35.0%
!!!	CTTGTAAGTACTTCTGGTTT+TGG	+	tig0021212:84661-84680	Msa1389120:intron	35.0%
	AAGATCCGAAGGCTTATATT+CGG	+	tig0021212:84794-84813	Msa1389120:intron	35.0%
	AAAGTCCGAATATAAGCCTT+CGG	-	tig0021212:84802-84821	None:intergenic	35.0%
	CTGCAACTGCAATGAATTAT+TGG	+	tig0021212:84887-84906	Msa1389120:intron	35.0%
	TATCAGGAATCAGATTCCTT+GGG	-	tig0021212:84914-84933	None:intergenic	35.0%
	ACATCCCTTTATGTACTATC+AGG	-	tig0021212:84930-84949	None:intergenic	35.0%
	GGGTATTATACATACATAGC+AGG	+	tig0021212:84982-85001	Msa1389120:intron	35.0%
	ATTATACATACATAGCAGGC+AGG	+	tig0021212:84986-85005	Msa1389120:intron	35.0%
	TCGACCAAGGAAAATAAGAA+AGG	-	tig0021212:85090-85109	None:intergenic	35.0%
!	CTCCTTTTCCTCAATCAAAA+CGG	-	tig0021212:85467-85486	None:intergenic	35.0%
	ATCCAAGTGCTAATTCAAAG+TGG	-	tig0021212:85547-85566	None:intergenic	35.0%
!	GTCAACTGAAAGATGGTTTT+TGG	+	tig0021212:85634-85653	Msa1389120:CDS	35.0%
	TTTATCAGTGTTACACAGTC+TGG	+	tig0021212:85832-85851	Msa1389120:intron	35.0%
	TTATCAGTGTTACACAGTCT+GGG	+	tig0021212:85833-85852	Msa1389120:intron	35.0%
!!	TGAAGCAGCTGATTTTCAAA+AGG	+	tig0021212:85909-85928	Msa1389120:CDS	35.0%
	TTTCTCATCAGGAATTTCGA+TGG	-	tig0021212:85995-86014	Msa1389130:three_prime_UTR	35.0%
!!!	AATTTTTTGGTCAGCTTCAG+AGG	-	tig0021212:86061-86080	Msa1389130:three_prime_UTR	35.0%
!!	GCCATAAAGAGAAGTTTTGT+TGG	+	tig0021212:86129-86148	Msa1389120:three_prime_UTR	35.0%
!!!	AGCAAGGGAATATTTTGATC+AGG	-	tig0021212:86262-86281	Msa1389130:three_prime_UTR	35.0%
	GAAAAACATAAGCACTCACT+GGG	-	tig0021212:84625-84644	None:intergenic	35.0%
!!!	CTTGTAAGTACTTCTGGTTT+TGG	+	tig0021212:84661-84680	Msa1389120:intron	35.0%
	AAGATCCGAAGGCTTATATT+CGG	+	tig0021212:84794-84813	Msa1389120:intron	35.0%
	AAAGTCCGAATATAAGCCTT+CGG	-	tig0021212:84802-84821	None:intergenic	35.0%
	CTGCAACTGCAATGAATTAT+TGG	+	tig0021212:84887-84906	Msa1389120:intron	35.0%
	TATCAGGAATCAGATTCCTT+GGG	-	tig0021212:84914-84933	None:intergenic	35.0%
	ACATCCCTTTATGTACTATC+AGG	-	tig0021212:84930-84949	None:intergenic	35.0%
	GGGTATTATACATACATAGC+AGG	+	tig0021212:84982-85001	Msa1389120:intron	35.0%
	ATTATACATACATAGCAGGC+AGG	+	tig0021212:84986-85005	Msa1389120:intron	35.0%
	TCGACCAAGGAAAATAAGAA+AGG	-	tig0021212:85090-85109	None:intergenic	35.0%
!	CTCCTTTTCCTCAATCAAAA+CGG	-	tig0021212:85467-85486	None:intergenic	35.0%
	ATCCAAGTGCTAATTCAAAG+TGG	-	tig0021212:85547-85566	None:intergenic	35.0%
!	GTCAACTGAAAGATGGTTTT+TGG	+	tig0021212:85634-85653	Msa1389120:CDS	35.0%
	TTTATCAGTGTTACACAGTC+TGG	+	tig0021212:85832-85851	Msa1389120:intron	35.0%
	TTATCAGTGTTACACAGTCT+GGG	+	tig0021212:85833-85852	Msa1389120:intron	35.0%
!!	TGAAGCAGCTGATTTTCAAA+AGG	+	tig0021212:85909-85928	Msa1389120:CDS	35.0%
	TTTCTCATCAGGAATTTCGA+TGG	-	tig0021212:85995-86014	Msa1389130:three_prime_UTR	35.0%
!!!	AATTTTTTGGTCAGCTTCAG+AGG	-	tig0021212:86061-86080	Msa1389130:three_prime_UTR	35.0%
!!	GCCATAAAGAGAAGTTTTGT+TGG	+	tig0021212:86129-86148	Msa1389120:three_prime_UTR	35.0%
!!!	AGCAAGGGAATATTTTGATC+AGG	-	tig0021212:86262-86281	Msa1389130:three_prime_UTR	35.0%
	GGAAAAACATAAGCACTCAC+TGG	-	tig0021212:84626-84645	None:intergenic	40.0%
	TCACAATACCAATTGTGTGG+TGG	+	tig0021212:84695-84714	Msa1389120:intron	40.0%
	ATCAGGAATCAGATTCCTTG+GGG	-	tig0021212:84913-84932	None:intergenic	40.0%
	CTATCAGGAATCAGATTCCT+TGG	-	tig0021212:84915-84934	None:intergenic	40.0%
!!!	TCTATGCACCGTTTTGATTG+AGG	+	tig0021212:85456-85475	Msa1389120:five_prime_UTR	40.0%
!!!	CACCGTTTTGATTGAGGAAA+AGG	+	tig0021212:85462-85481	Msa1389120:five_prime_UTR	40.0%
!!!	CGTTTTGATTGAGGAAAAGG+AGG	+	tig0021212:85465-85484	Msa1389120:five_prime_UTR	40.0%
!!	TGCCACTTTGAATTAGCACT+TGG	+	tig0021212:85542-85561	Msa1389120:five_prime_UTR	40.0%
	TGAGAAGGTCAACTGAAAGA+TGG	+	tig0021212:85627-85646	Msa1389120:CDS	40.0%
!!!	TGAAAGATGGTTTTTGGCCA+AGG	+	tig0021212:85640-85659	Msa1389120:CDS	40.0%
	CTGATTACTCGGTCTAGTAT+CGG	-	tig0021212:85725-85744	Msa1389130:three_prime_UTR	40.0%
	TCTTGACCAGTCTGATTACT+CGG	-	tig0021212:85736-85755	Msa1389130:three_prime_UTR	40.0%
	GGATAGGGTTGAGCTTATTA+TGG	+	tig0021212:85951-85970	Msa1389120:CDS	40.0%
	AGCTTATCTGCTTTCTCATC+AGG	-	tig0021212:86006-86025	Msa1389130:three_prime_UTR	40.0%
	GGCTATGTACTTTGCAACAT+CGG	-	tig0021212:86040-86059	Msa1389130:three_prime_UTR	40.0%
!!	GTTTTGTTGGTTGAGCTGTT+TGG	+	tig0021212:86142-86161	Msa1389120:three_prime_UTR	40.0%
!	TCAGGTGTCATGCTTACATT+GGG	-	tig0021212:86244-86263	Msa1389130:three_prime_UTR	40.0%
!	ATCAGGTGTCATGCTTACAT+TGG	-	tig0021212:86245-86264	Msa1389130:three_prime_UTR	40.0%
	GGAAAAACATAAGCACTCAC+TGG	-	tig0021212:84626-84645	None:intergenic	40.0%
	TCACAATACCAATTGTGTGG+TGG	+	tig0021212:84695-84714	Msa1389120:intron	40.0%
	ATCAGGAATCAGATTCCTTG+GGG	-	tig0021212:84913-84932	None:intergenic	40.0%
	CTATCAGGAATCAGATTCCT+TGG	-	tig0021212:84915-84934	None:intergenic	40.0%
!!!	TCTATGCACCGTTTTGATTG+AGG	+	tig0021212:85456-85475	Msa1389120:five_prime_UTR	40.0%
!!!	CACCGTTTTGATTGAGGAAA+AGG	+	tig0021212:85462-85481	Msa1389120:five_prime_UTR	40.0%
!!!	CGTTTTGATTGAGGAAAAGG+AGG	+	tig0021212:85465-85484	Msa1389120:five_prime_UTR	40.0%
!!	TGCCACTTTGAATTAGCACT+TGG	+	tig0021212:85542-85561	Msa1389120:five_prime_UTR	40.0%
	TGAGAAGGTCAACTGAAAGA+TGG	+	tig0021212:85627-85646	Msa1389120:CDS	40.0%
!!!	TGAAAGATGGTTTTTGGCCA+AGG	+	tig0021212:85640-85659	Msa1389120:CDS	40.0%
	CTGATTACTCGGTCTAGTAT+CGG	-	tig0021212:85725-85744	Msa1389130:three_prime_UTR	40.0%
	TCTTGACCAGTCTGATTACT+CGG	-	tig0021212:85736-85755	Msa1389130:three_prime_UTR	40.0%
	GGATAGGGTTGAGCTTATTA+TGG	+	tig0021212:85951-85970	Msa1389120:CDS	40.0%
	AGCTTATCTGCTTTCTCATC+AGG	-	tig0021212:86006-86025	Msa1389130:three_prime_UTR	40.0%
	GGCTATGTACTTTGCAACAT+CGG	-	tig0021212:86040-86059	Msa1389130:three_prime_UTR	40.0%
!!	GTTTTGTTGGTTGAGCTGTT+TGG	+	tig0021212:86142-86161	Msa1389120:three_prime_UTR	40.0%
!	TCAGGTGTCATGCTTACATT+GGG	-	tig0021212:86244-86263	Msa1389130:three_prime_UTR	40.0%
!	ATCAGGTGTCATGCTTACAT+TGG	-	tig0021212:86245-86264	Msa1389130:three_prime_UTR	40.0%
	AGCCTTCGGATCTTCAATCA+GGG	-	tig0021212:84788-84807	None:intergenic	45.0%
	AAGCCTTCGGATCTTCAATC+AGG	-	tig0021212:84789-84808	None:intergenic	45.0%
	GCAATGAATTATTGGCCCCA+AGG	+	tig0021212:84895-84914	Msa1389120:intron	45.0%
	ACTAGACCGAGTAATCAGAC+TGG	+	tig0021212:85727-85746	Msa1389120:CDS	45.0%
	TAAACCTGGACAGCTTGGAT+AGG	+	tig0021212:85935-85954	Msa1389120:CDS	45.0%
	AAACCTGGACAGCTTGGATA+GGG	+	tig0021212:85936-85955	Msa1389120:CDS	45.0%
	TGTCATGCTTACATTGGGGT+GGG	-	tig0021212:86239-86258	Msa1389130:three_prime_UTR	45.0%
!	CAGGTGTCATGCTTACATTG+GGG	-	tig0021212:86243-86262	Msa1389130:three_prime_UTR	45.0%
	AGCCTTCGGATCTTCAATCA+GGG	-	tig0021212:84788-84807	None:intergenic	45.0%
	AAGCCTTCGGATCTTCAATC+AGG	-	tig0021212:84789-84808	None:intergenic	45.0%
	GCAATGAATTATTGGCCCCA+AGG	+	tig0021212:84895-84914	Msa1389120:intron	45.0%
	ACTAGACCGAGTAATCAGAC+TGG	+	tig0021212:85727-85746	Msa1389120:CDS	45.0%
	TAAACCTGGACAGCTTGGAT+AGG	+	tig0021212:85935-85954	Msa1389120:CDS	45.0%
	AAACCTGGACAGCTTGGATA+GGG	+	tig0021212:85936-85955	Msa1389120:CDS	45.0%
	TGTCATGCTTACATTGGGGT+GGG	-	tig0021212:86239-86258	Msa1389130:three_prime_UTR	45.0%
!	CAGGTGTCATGCTTACATTG+GGG	-	tig0021212:86243-86262	Msa1389130:three_prime_UTR	45.0%
	CACTCACTGGGAGATTCGTT+TGG	-	tig0021212:84613-84632	None:intergenic	50.0%
	TCTGCGTACCACCACACAAT+TGG	-	tig0021212:84706-84725	None:intergenic	50.0%
	TCCCCTGATTGAAGATCCGA+AGG	+	tig0021212:84783-84802	Msa1389120:intron	50.0%
	GCCTTCGGATCTTCAATCAG+GGG	-	tig0021212:84787-84806	None:intergenic	50.0%
!	AGATGTTGGTGCTCTTCAGC+AGG	+	tig0021212:85686-85705	Msa1389120:CDS	50.0%
!	GTCTAGTATCGGATCAGAGC+TGG	-	tig0021212:85714-85733	Msa1389130:three_prime_UTR	50.0%
	GGACTTAAACCTGGACAGCT+TGG	+	tig0021212:85930-85949	Msa1389120:CDS	50.0%
	GTCATGCTTACATTGGGGTG+GGG	-	tig0021212:86238-86257	Msa1389130:three_prime_UTR	50.0%
	GTGTCATGCTTACATTGGGG+TGG	-	tig0021212:86240-86259	Msa1389130:three_prime_UTR	50.0%
	CACTCACTGGGAGATTCGTT+TGG	-	tig0021212:84613-84632	None:intergenic	50.0%
	TCTGCGTACCACCACACAAT+TGG	-	tig0021212:84706-84725	None:intergenic	50.0%
	TCCCCTGATTGAAGATCCGA+AGG	+	tig0021212:84783-84802	Msa1389120:intron	50.0%
	GCCTTCGGATCTTCAATCAG+GGG	-	tig0021212:84787-84806	None:intergenic	50.0%
!	AGATGTTGGTGCTCTTCAGC+AGG	+	tig0021212:85686-85705	Msa1389120:CDS	50.0%
!	GTCTAGTATCGGATCAGAGC+TGG	-	tig0021212:85714-85733	Msa1389130:three_prime_UTR	50.0%
	GGACTTAAACCTGGACAGCT+TGG	+	tig0021212:85930-85949	Msa1389120:CDS	50.0%
	GTCATGCTTACATTGGGGTG+GGG	-	tig0021212:86238-86257	Msa1389130:three_prime_UTR	50.0%
	GTGTCATGCTTACATTGGGG+TGG	-	tig0021212:86240-86259	Msa1389130:three_prime_UTR	50.0%
	CAACCCTATCCAAGCTGTCC+AGG	-	tig0021212:85942-85961	Msa1389130:three_prime_UTR	55.0%
	CAACCCTATCCAAGCTGTCC+AGG	-	tig0021212:85942-85961	Msa1389130:three_prime_UTR	55.0%

Chromosome	Type	Strat	End	Strand	Name
tig0021212	gene	84555	86319	84555	ID=Msa1389120;Name=Msa1389120
tig0021212	mRNA	84555	86319	84555	ID=Msa1389120-mRNA-1;Parent=Msa1389120;Name=Msa1389120-mRNA-1;_AED=0.01;_eAED=0.01;_QI=204\|1\|1\|1\|0.5\|0.33\|3\|236\|124
tig0021212	exon	84555	84625	84555	ID=Msa1389120-mRNA-1:exon:11449;Parent=Msa1389120-mRNA-1
tig0021212	exon	85454	85781	85454	ID=Msa1389120-mRNA-1:exon:11450;Parent=Msa1389120-mRNA-1
tig0021212	exon	85904	86319	85904	ID=Msa1389120-mRNA-1:exon:11451;Parent=Msa1389120-mRNA-1
tig0021212	five_prime_UTR	84555	84625	84555	ID=Msa1389120-mRNA-1:five_prime_utr;Parent=Msa1389120-mRNA-1
tig0021212	five_prime_UTR	85454	85586	85454	ID=Msa1389120-mRNA-1:five_prime_utr;Parent=Msa1389120-mRNA-1
tig0021212	CDS	85587	85781	85587	ID=Msa1389120-mRNA-1:cds;Parent=Msa1389120-mRNA-1
tig0021212	CDS	85904	86083	85904	ID=Msa1389120-mRNA-1:cds;Parent=Msa1389120-mRNA-1
tig0021212	three_prime_UTR	86084	86319	86084	ID=Msa1389120-mRNA-1:three_prime_utr;Parent=Msa1389120-mRNA-1

Gene Sequence	>Msa1389120 ATGCAAAGCATAAAGAAATCTATACTAAGTCATGTGAATTTGAGAAGGTCAACTGAAAGATGGTTTTTGGCCAAGGATGATAATGTGATTATGCATTTGAGATGTTGGTGCTCTTCAGCAGGTGCCAGCTCTGATCCGATACTAGACCGAGTAATCAGACTGGTCAAGAAATATGATAAAATTAATGCAGCAAAGGTTACTGAAGCAGCTGATTTTCAAAAGGACTTAAACCTGGACAGCTTGGATAGGGTTGAGCTTATTATGGCTCTTGAAGAAGAATTTTCCATCGAAATTCCTGATGAGAAAGCAGATAAGCTTACTTGCTGTACCGATGTTGCAAAGTACATAGCCTCTGAAGCTGACCAAAAAATTTAG
Protein sequence	>Msa1389120 MQSIKKSILSHVNLRRSTERWFLAKDDNVIMHLRCWCSSAGASSDPILDRVIRLVKKYDKINAAKVTEAADFQKDLNLDSLDRVELIMALEEEFSIEIPDEKADKLTCCTDVAKYIASEADQKI*