AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011716

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011716.t1 MTR_4g097090 92.998 457 32 0 1 457 1 457 0.0 880
MS.gene011716.t1 MTR_4g127650 79.333 450 90 3 9 457 10 457 0.0 734
MS.gene011716.t1 MTR_3g026950 43.016 451 237 9 17 456 10 451 3.58e-107 325
MS.gene011716.t1 MTR_3g049150 40.481 457 252 7 9 456 2 447 4.12e-106 323
MS.gene011716.t1 MTR_4g007540 37.862 449 255 9 9 453 3 431 6.78e-96 296
MS.gene011716.t1 MTR_4g013050 39.348 460 222 13 3 456 2 410 1.31e-93 289
MS.gene011716.t1 MTR_2g064495 37.639 449 255 9 9 453 3 430 1.03e-92 287
MS.gene011716.t1 MTR_8g075610 38.208 424 220 9 41 453 26 418 3.99e-92 285
MS.gene011716.t1 MTR_2g105330 37.416 449 256 9 9 453 3 430 9.65e-91 282
MS.gene011716.t1 MTR_7g085930 35.106 470 269 14 9 456 6 461 2.46e-76 246
MS.gene011716.t1 MTR_7g086000 35.841 452 272 10 17 456 12 457 1.10e-74 242
MS.gene011716.t1 MTR_7g085990 34.043 470 274 15 9 456 2 457 1.07e-71 234
MS.gene011716.t1 MTR_3g049140 43.802 242 133 3 215 456 6 244 1.80e-64 208
MS.gene011716.t1 MTR_8g096530 30.543 442 273 10 15 451 13 425 1.44e-50 177
MS.gene011716.t1 MTR_7g027175 30.788 406 240 11 15 411 22 395 2.98e-47 169
MS.gene011716.t1 MTR_1g053315 29.581 453 282 13 19 457 33 462 5.01e-47 169
MS.gene011716.t1 MTR_3g098980 29.500 400 248 11 17 411 22 392 1.13e-43 159
MS.gene011716.t1 MTR_7g084940 25.926 459 301 10 9 457 16 445 3.58e-38 144
MS.gene011716.t1 MTR_7g114520 28.723 470 287 16 1 452 1 440 8.93e-36 138
MS.gene011716.t1 MTR_7g085960 35.025 197 108 4 262 456 12 190 5.09e-33 124
MS.gene011716.t1 MTR_7g114470 25.986 431 272 12 34 448 37 436 1.69e-32 129
MS.gene011716.t1 MTR_1g081660 27.252 444 264 16 30 452 39 444 1.84e-32 128
MS.gene011716.t1 MTR_7g056087 23.820 445 301 12 17 448 12 431 2.33e-32 128
MS.gene011716.t1 MTR_7g114490 25.874 429 275 12 34 449 36 434 2.44e-32 129
MS.gene011716.t1 MTR_1g040215 28.074 431 249 17 1 411 1 390 2.81e-32 128
MS.gene011716.t1 MTR_1g040175 27.628 409 251 12 17 413 17 392 2.87e-32 128
MS.gene011716.t1 MTR_5g076700 25.404 433 279 13 34 452 36 438 2.91e-32 128
MS.gene011716.t1 MTR_3g071320 26.350 463 295 15 7 454 3 434 8.10e-32 126
MS.gene011716.t1 MTR_7g114560 25.499 451 290 14 17 452 18 437 2.76e-31 125
MS.gene011716.t1 MTR_6g045553 25.988 481 277 11 6 441 11 457 3.14e-31 125
MS.gene011716.t1 MTR_7g084910 25.604 414 260 9 2 409 10 381 3.42e-31 124
MS.gene011716.t1 MTR_5g081960 25.646 503 293 16 1 449 1 476 4.00e-31 125
MS.gene011716.t1 MTR_5g090830 26.407 462 296 14 19 456 23 464 4.31e-31 125
MS.gene011716.t1 MTR_5g090810 25.966 466 293 16 19 456 23 464 1.09e-30 124
MS.gene011716.t1 MTR_7g114550 25.404 433 278 13 34 452 36 437 2.27e-30 122
MS.gene011716.t1 MTR_4g015180 26.358 497 282 19 1 441 1 469 8.41e-30 121
MS.gene011716.t1 MTR_3g112180 28.395 405 234 13 65 449 61 429 1.65e-29 120
MS.gene011716.t1 MTR_1g040195 26.464 461 272 16 1 448 1 407 2.79e-29 119
MS.gene011716.t1 MTR_4g055170 26.681 476 292 17 4 452 7 452 7.35e-29 118
MS.gene011716.t1 MTR_3g070170 25.410 488 272 15 3 440 6 451 1.30e-28 118
MS.gene011716.t1 MTR_3g101960 27.027 444 268 16 30 449 29 440 1.98e-27 114
MS.gene011716.t1 MTR_8g079970 26.503 449 279 18 17 451 11 422 3.43e-27 113
MS.gene011716.t1 MTR_8g041770 25.275 455 272 18 18 447 18 429 2.16e-26 111
MS.gene011716.t1 MTR_3g102020 27.500 400 238 13 61 440 65 432 2.93e-26 111
MS.gene011716.t1 MTR_1g040320 26.608 451 251 17 1 438 1 384 3.08e-26 110
MS.gene011716.t1 MTR_1g094790 26.603 421 260 16 30 432 26 415 3.49e-26 110
MS.gene011716.t1 MTR_8g079950 26.316 456 259 17 22 451 18 422 4.91e-26 110
MS.gene011716.t1 MTR_5g084870 26.174 447 280 18 17 452 11 418 6.28e-26 109
MS.gene011716.t1 MTR_2g015430 26.500 400 259 10 63 450 56 432 9.85e-26 109
MS.gene011716.t1 MTR_3g102010 26.402 428 273 16 30 440 30 432 2.71e-25 108
MS.gene011716.t1 MTR_3g101970 25.935 428 262 15 30 440 29 418 5.89e-25 107
MS.gene011716.t1 MTR_4g094835 25.445 393 247 12 65 440 64 427 3.26e-24 105
MS.gene011716.t1 MTR_3g101930 25.843 445 273 15 5 421 3 418 5.17e-24 104
MS.gene011716.t1 MTR_5g084850 23.947 451 284 14 17 452 11 417 6.84e-24 103
MS.gene011716.t1 MTR_8g079980 25.618 445 290 16 16 451 10 422 9.53e-24 103
MS.gene011716.t1 MTR_5g090880 25.527 427 274 15 56 456 58 466 1.58e-23 103
MS.gene011716.t1 MTR_3g450710 23.844 411 267 11 61 446 61 450 1.83e-23 103
MS.gene011716.t1 MTR_0190s0020 26.031 388 248 15 65 440 63 423 7.22e-23 100
MS.gene011716.t1 MTR_3g101900 23.568 454 298 12 16 449 14 438 9.38e-23 100
MS.gene011716.t1 MTR_4g094838 25.450 389 238 12 65 440 64 413 1.95e-22 99.8
MS.gene011716.t1 MTR_3g450690 23.786 412 263 12 61 446 68 454 3.59e-22 99.4
MS.gene011716.t1 MTR_3g026310 24.000 450 279 16 27 447 21 436 6.71e-22 98.2
MS.gene011716.t1 MTR_1g040315 26.331 338 219 11 4 331 2 319 9.31e-22 97.4
MS.gene011716.t1 MTR_3g088420 24.554 448 285 18 27 457 21 432 2.72e-21 96.3
MS.gene011716.t1 MTR_4g094832 23.662 355 234 9 95 440 85 411 3.00e-21 96.3
MS.gene011716.t1 MTR_4g094842 26.551 403 234 15 65 440 62 429 3.42e-21 95.9
MS.gene011716.t1 MTR_2g045540 43.478 115 53 1 215 329 6 108 6.75e-21 88.2
MS.gene011716.t1 MTR_2g045550 39.161 143 76 2 9 151 4 135 8.16e-21 89.0
MS.gene011716.t1 MTR_3g465990 26.857 350 219 13 12 342 13 344 1.33e-20 94.4
MS.gene011716.t1 MTR_3g465930 27.729 339 223 12 17 342 18 347 3.49e-20 93.2
MS.gene011716.t1 MTR_7g114960 25.688 327 217 8 45 363 45 353 1.34e-19 91.3
MS.gene011716.t1 MTR_5g026320 25.199 377 237 13 42 405 53 397 2.62e-19 90.5
MS.gene011716.t1 MTR_8g467580 24.764 424 279 15 17 427 11 407 3.93e-19 89.7
MS.gene011716.t1 MTR_7g110610 25.618 445 245 18 17 453 13 379 6.92e-19 88.6
MS.gene011716.t1 MTR_4g022290 27.518 407 237 20 30 414 30 400 8.94e-19 88.6
MS.gene011716.t1 MTR_3g465910 27.957 372 221 18 17 363 18 367 1.22e-18 89.0
MS.gene011716.t1 MTR_3g465950 30.000 270 159 10 65 318 68 323 3.75e-18 87.0
MS.gene011716.t1 MTR_4g022720 24.505 404 251 15 65 448 55 424 4.62e-18 86.7
MS.gene011716.t1 MTR_4g022800 24.557 395 253 13 65 440 55 423 5.50e-18 86.3
MS.gene011716.t1 MTR_3g028210 23.820 466 309 15 14 452 14 460 9.84e-18 85.9
MS.gene011716.t1 MTR_8g467590 24.169 451 303 19 12 451 6 428 1.01e-17 85.5
MS.gene011716.t1 MTR_4g022825 24.018 433 265 14 4 414 8 398 1.62e-17 85.1
MS.gene011716.t1 MTR_3g465980 25.360 347 228 12 12 342 13 344 2.73e-17 84.3
MS.gene011716.t1 MTR_7g026860 24.215 446 253 19 30 440 24 419 3.15e-17 84.0
MS.gene011716.t1 MTR_2g089735 23.691 401 253 13 40 408 37 416 1.03e-16 82.8
MS.gene011716.t1 MTR_8g079710 24.942 433 272 21 28 440 27 426 1.03e-16 82.4
MS.gene011716.t1 MTR_4g094828 25.679 405 240 18 65 440 59 431 2.33e-16 81.3
MS.gene011716.t1 MTR_7g014160 23.904 502 307 20 1 456 1 473 3.78e-16 80.9
MS.gene011716.t1 MTR_7g014200 23.770 488 316 18 1 454 1 466 1.02e-15 79.7
MS.gene011716.t1 MTR_7g085970 42.308 78 44 1 102 179 34 110 2.57e-15 72.8
MS.gene011716.t1 MTR_4g007460 36.283 113 64 2 79 191 1 105 1.72e-14 71.2
MS.gene011716.t1 MTR_2g036780 23.410 393 222 15 68 431 62 404 1.08e-13 73.2
MS.gene011716.t1 MTR_7g114510 25.237 317 196 11 147 450 8 296 2.28e-13 71.2
MS.gene011716.t1 MTR_7g114500 35.000 120 73 3 34 151 36 152 8.38e-13 66.2
MS.gene011716.t1 MTR_2g089650 24.190 401 248 15 41 408 41 418 1.32e-12 70.1
MS.gene011716.t1 MTR_0042s0190 30.189 159 91 5 258 413 22 163 1.73e-12 67.4
MS.gene011716.t1 MTR_7g014610 22.547 479 313 16 17 456 16 475 1.85e-12 69.3
MS.gene011716.t1 MTR_0042s0190 30.189 159 91 5 258 413 87 228 3.26e-12 67.4
MS.gene011716.t1 MTR_7g014330 22.927 410 260 15 28 406 24 408 2.52e-11 65.9
MS.gene011716.t1 MTR_3g100150 23.399 406 245 16 65 449 65 425 2.61e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011716.t1 AT2G19070 54.148 458 190 8 9 456 4 451 1.65e-174 497
MS.gene011716.t1 AT5G48930 37.665 454 251 11 9 454 3 432 4.34e-95 293
MS.gene011716.t1 AT5G57840 31.947 457 277 12 9 454 3 436 6.10e-66 218
MS.gene011716.t1 AT5G41040 31.320 447 271 11 15 454 23 440 1.89e-56 193
MS.gene011716.t1 AT5G41040 31.320 447 271 11 15 454 39 456 3.57e-56 193
MS.gene011716.t1 AT3G48720 30.886 463 270 13 1 450 1 426 4.56e-51 179
MS.gene011716.t1 AT5G63560 31.533 463 261 14 1 449 1 421 1.63e-48 172
MS.gene011716.t1 AT5G17540 27.682 466 293 15 1 452 1 436 1.08e-38 146
MS.gene011716.t1 AT1G65450 27.214 463 292 13 9 457 18 449 1.13e-37 143
MS.gene011716.t1 AT5G07080 26.503 449 292 13 17 452 22 445 7.09e-35 135
MS.gene011716.t1 AT1G27620 26.606 436 277 10 15 442 15 415 1.10e-34 134
MS.gene011716.t1 AT2G40230 25.612 449 298 9 9 449 5 425 1.16e-34 134
MS.gene011716.t1 AT1G03390 27.451 459 274 15 17 448 25 451 2.54e-32 128
MS.gene011716.t1 AT3G26040 28.604 444 273 19 17 450 11 420 9.65e-32 126
MS.gene011716.t1 AT3G03480 26.402 428 264 13 1 411 7 400 1.34e-31 126
MS.gene011716.t1 AT1G32910 25.553 407 265 14 40 433 44 425 2.43e-31 125
MS.gene011716.t1 AT4G31910 25.618 445 261 13 44 452 45 455 1.74e-28 117
MS.gene011716.t1 AT3G30280 28.889 450 258 21 22 451 21 428 1.34e-27 114
MS.gene011716.t1 AT5G23940 25.702 463 281 13 15 446 9 439 3.55e-27 114
MS.gene011716.t1 AT3G50270 25.513 439 266 15 9 420 6 410 4.95e-27 113
MS.gene011716.t1 AT2G39980 26.526 426 237 14 65 440 62 461 6.41e-27 113
MS.gene011716.t1 AT2G25150 26.432 454 301 16 12 452 16 449 8.89e-27 112
MS.gene011716.t1 AT5G16410 24.471 425 262 16 28 432 59 444 1.21e-26 112
MS.gene011716.t1 AT5G07860 26.866 469 293 15 1 449 1 439 1.24e-26 112
MS.gene011716.t1 AT1G24430 27.296 392 234 18 30 411 29 379 1.36e-26 111
MS.gene011716.t1 AT1G78990 25.628 398 261 13 64 450 66 439 2.59e-26 111
MS.gene011716.t1 AT5G01210 25.954 393 248 12 76 440 73 450 3.78e-26 110
MS.gene011716.t1 AT3G50300 26.521 411 258 14 45 436 35 420 1.08e-25 109
MS.gene011716.t1 AT5G42830 26.757 441 271 14 30 449 30 439 4.09e-25 107
MS.gene011716.t1 AT3G62160 23.239 426 281 11 36 450 26 416 4.00e-24 104
MS.gene011716.t1 AT1G28680 26.716 408 259 14 57 450 57 438 3.21e-23 102
MS.gene011716.t1 AT1G24420 29.551 379 227 16 76 449 76 419 4.02e-23 101
MS.gene011716.t1 AT1G24420 29.551 379 227 16 76 449 76 419 4.02e-23 101
MS.gene011716.t1 AT5G47980 28.070 456 261 19 17 448 13 425 4.43e-23 101
MS.gene011716.t1 AT5G67150 25.917 436 267 16 27 440 26 427 4.61e-23 101
MS.gene011716.t1 AT3G50280 25.926 378 239 11 59 420 53 405 5.44e-23 101
MS.gene011716.t1 AT3G47170 26.362 459 299 17 17 452 20 462 1.65e-22 100
MS.gene011716.t1 AT5G38130 25.499 451 277 17 9 436 24 438 1.67e-22 100
MS.gene011716.t1 AT4G15390 24.667 450 290 14 17 451 16 431 1.88e-22 99.8
MS.gene011716.t1 AT4G15390 24.667 450 290 14 17 451 16 431 1.88e-22 99.8
MS.gene011716.t1 AT5G47950 27.209 430 257 18 13 432 10 393 3.03e-22 99.0
MS.gene011716.t1 AT2G23510 24.213 413 254 14 65 452 69 447 4.82e-22 98.6
MS.gene011716.t1 AT5G07870 27.044 477 294 17 1 449 1 451 1.65e-21 97.1
MS.gene011716.t1 AT1G28680 26.557 305 209 8 57 357 57 350 2.18e-20 93.2
MS.gene011716.t1 AT3G29670 25.054 463 267 18 27 452 26 445 3.55e-19 90.1
MS.gene011716.t1 AT5G07850 26.420 405 247 14 65 449 70 443 3.05e-18 87.4
MS.gene011716.t1 AT5G67160 25.000 436 257 15 21 426 20 415 7.09e-18 85.9
MS.gene011716.t1 AT3G50280 26.042 288 180 8 141 420 13 275 1.01e-16 81.3
MS.gene011716.t1 AT3G50290 26.263 297 178 10 125 407 2 271 8.16e-16 77.8
MS.gene011716.t1 AT4G15400 25.727 447 283 17 13 448 9 417 1.24e-14 76.3
MS.gene011716.t1 AT3G29635 24.379 443 290 19 28 448 27 446 1.66e-14 75.9
MS.gene011716.t1 AT1G65450 25.974 231 137 6 236 457 80 285 7.72e-14 72.4
MS.gene011716.t1 AT1G65450 32.857 140 89 4 9 146 18 154 1.12e-12 66.2
MS.gene011716.t1 AT5G61160 24.292 424 260 19 59 454 58 448 9.63e-12 67.4
MS.gene011716.t1 AT5G39050 23.846 390 237 15 99 456 106 467 6.39e-11 64.7

Find 112 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTACCAAAAGGGTATTT+TGG 0.102364 8.3:-22149010 MS.gene011716:CDS
CCAATTTATGGACTTGATTT+TGG 0.108014 8.3:-22148737 MS.gene011716:CDS
TGGTTGTCATTGCCAATTTA+TGG 0.127883 8.3:-22148749 MS.gene011716:CDS
GATCCATATGGATGCCTTTA+AGG 0.198031 8.3:-22148597 MS.gene011716:CDS
TTAACATTGTTACGACATTT+TGG 0.220212 8.3:+22149322 None:intergenic
AAGCAGTAACCGGTCATATT+TGG 0.239075 8.3:-22149120 MS.gene011716:CDS
GATCAACTTCCTTTATTGTT+AGG 0.251787 8.3:-22149286 MS.gene011716:CDS
CGCTTGGTGTTTGCGTTGAT+TGG 0.258434 8.3:-22149051 MS.gene011716:CDS
CAACTAACCACTCCTAAATT+AGG 0.269802 8.3:+22148770 None:intergenic
TCTGAATTTCCTAACAATAA+AGG 0.299564 8.3:+22149277 None:intergenic
CCAAAATCAAGTCCATAAAT+TGG 0.313583 8.3:+22148737 None:intergenic
ATCTGATCCCATTCGGATAA+TGG 0.324028 8.3:+22149838 None:intergenic
AAATTAGGATTCCAATAAAA+AGG 0.326383 8.3:+22148785 None:intergenic
CGATGATGATGGTTCTATAT+TGG 0.355288 8.3:-22148639 MS.gene011716:CDS
ATGTGGTGAGCCTTTGAAAA+TGG 0.355480 8.3:-22149377 MS.gene011716:CDS
TCCCTACAACTTGAAGATCT+TGG 0.363159 8.3:+22148822 None:intergenic
GCATTCCCAAAATACCCTTT+TGG 0.365952 8.3:+22149004 None:intergenic
AGTAGCCAAAACATCCAAAA+TGG 0.383532 8.3:+22148982 None:intergenic
AACAATAAAGGAAGTTGATC+AGG 0.389462 8.3:+22149289 None:intergenic
GATATAATGTCCAAACCCTT+AGG 0.390597 8.3:-22148947 MS.gene011716:CDS
GAATGACCCCGACCATTACT+AGG 0.395098 8.3:+22149151 None:intergenic
TGATCTCGAATGCAACTCAT+TGG 0.407126 8.3:-22149650 MS.gene011716:CDS
CCACTTGCCGGTCGTTTACA+CGG 0.416496 8.3:-22149691 MS.gene011716:CDS
TTCCAAGATCTTCAAGTTGT+AGG 0.420035 8.3:-22148824 MS.gene011716:CDS
GTCGTAACAATGTTAATGAA+TGG 0.427383 8.3:-22149315 MS.gene011716:CDS
AGGTGAAAAGTCACCTAGTT+CGG 0.428468 8.3:+22149588 None:intergenic
GTGGAACCTAATTTACCAAA+AGG 0.435496 8.3:-22149019 MS.gene011716:CDS
CTAATTTAGGAGTGGTTAGT+TGG 0.435977 8.3:-22148769 MS.gene011716:CDS
GAATAAACCTAGTAATGGTC+GGG 0.442531 8.3:-22149158 MS.gene011716:CDS
TGAAGCATACCCTAAGGGTT+TGG 0.444962 8.3:+22148937 None:intergenic
AATGATGAGTATGTTAGGTT+GGG 0.453061 8.3:-22148884 MS.gene011716:CDS
GGTATGCTTCAAGTAGGATA+AGG 0.453543 8.3:-22148925 MS.gene011716:CDS
TGTAATCAACTTGTGGGATA+AGG 0.455844 8.3:+22149545 None:intergenic
TCGTAACAATGTTAATGAAT+GGG 0.463530 8.3:-22149314 MS.gene011716:CDS
CTTCCTAGTCCCGATGATGA+TGG 0.463543 8.3:-22148650 MS.gene011716:CDS
GATCTCGAATGCAACTCATT+GGG 0.466117 8.3:-22149649 MS.gene011716:CDS
GCTCCTTAAAGGCATCCATA+TGG 0.467393 8.3:+22148594 None:intergenic
TTTATTCCAACTCTCATTTG+CGG 0.467692 8.3:+22149174 None:intergenic
TCCAAGATCTTCAAGTTGTA+GGG 0.468030 8.3:-22148823 MS.gene011716:CDS
GAATGATGAGTATGTTAGGT+TGG 0.471062 8.3:-22148885 MS.gene011716:CDS
GGAACACATGAAGAACTTGA+TGG 0.478823 8.3:-22148686 MS.gene011716:CDS
GTATGCTTCAAGTAGGATAA+GGG 0.479099 8.3:-22148924 MS.gene011716:CDS
AATGACCAACCAACCGCGCT+TGG 0.479297 8.3:-22149067 MS.gene011716:CDS
CCGCACCATGTTGGTTCCAT+TGG 0.480928 8.3:+22149868 None:intergenic
TCGAGGTATGAAGCAGTAAC+CGG 0.483627 8.3:-22149130 MS.gene011716:CDS
CACTTGCCGGTCGTTTACAC+GGG 0.488481 8.3:-22149690 MS.gene011716:CDS
AAGTGAATGATGAGTATGTT+AGG 0.491531 8.3:-22148889 MS.gene011716:CDS
TCGTTTACCATTATCCGAAT+GGG 0.493784 8.3:-22149845 MS.gene011716:CDS
TTGTCTGCAAGAGATCCATA+TGG 0.500703 8.3:-22148609 MS.gene011716:CDS
ATATAATGTCCAAACCCTTA+GGG 0.507169 8.3:-22148946 MS.gene011716:CDS
TCCTTTCCCGTGTAAACGAC+CGG 0.508630 8.3:+22149684 None:intergenic
GAACCATCATCATCGGGACT+AGG 0.512386 8.3:+22148647 None:intergenic
TAACTGTAATATTCCAAAGA+AGG 0.512720 8.3:+22149568 None:intergenic
GATTCCAATCTGATCCCATT+CGG 0.514466 8.3:+22149831 None:intergenic
CCTACTTGAAGCATACCCTA+AGG 0.516468 8.3:+22148931 None:intergenic
CGACCGGCAAGTGGATAAAA+CGG 0.518498 8.3:+22149700 None:intergenic
GGAGTGTGTAATCAACTTGT+GGG 0.520503 8.3:+22149539 None:intergenic
GTCGTTTACCATTATCCGAA+TGG 0.522947 8.3:-22149846 MS.gene011716:CDS
CCAATGGAATATCTTCCATC+GGG 0.526580 8.3:+22149518 None:intergenic
ATTCAGATAATGTTGAAGAA+AGG 0.528429 8.3:-22149261 MS.gene011716:CDS
GATCTTCAAGTTGTAGGGAA+TGG 0.530205 8.3:-22148818 MS.gene011716:CDS
TTGGTGTTTGCGTTGATTGG+AGG 0.530584 8.3:-22149048 MS.gene011716:CDS
TCGTTAACTTTATCCGAACT+AGG 0.531806 8.3:-22149601 MS.gene011716:CDS
AAAATGAAATGCAACGCGCT+TGG 0.536125 8.3:+22149418 None:intergenic
TGGAACCTAATTTACCAAAA+GGG 0.539334 8.3:-22149018 MS.gene011716:CDS
GTTGTAGGGAATGGTGAGAA+GGG 0.540329 8.3:-22148809 MS.gene011716:CDS
ATCTCACATGCAGTTGCAGA+TGG 0.540526 8.3:-22149442 MS.gene011716:CDS
CCCGATGGAAGATATTCCAT+TGG 0.541022 8.3:-22149518 MS.gene011716:CDS
CTACTTGAAGCATACCCTAA+GGG 0.541120 8.3:+22148932 None:intergenic
AATATAGAACCATCATCATC+GGG 0.541670 8.3:+22148641 None:intergenic
AAGGAGGTTTATATGGGGCT+AGG 0.542309 8.3:-22148707 MS.gene011716:CDS
GGAATAAACCTAGTAATGGT+CGG 0.543778 8.3:-22149159 MS.gene011716:CDS
CCGTGTAAACGACCGGCAAG+TGG 0.543864 8.3:+22149691 None:intergenic
ACCAATGGAATATCTTCCAT+CGG 0.544804 8.3:+22149517 None:intergenic
CAATATAGAACCATCATCAT+CGG 0.546460 8.3:+22148640 None:intergenic
ACGCAAACACCAAGCGCGGT+TGG 0.546925 8.3:+22149058 None:intergenic
GGGTGGTAAAAGTATATTGT+TGG 0.548512 8.3:+22149796 None:intergenic
ATGTTTGTATTGAGAAACCT+TGG 0.549573 8.3:+22149352 None:intergenic
AGCCTTTGAAAATGGTGCCA+AGG 0.550882 8.3:-22149369 MS.gene011716:CDS
CGTTTACACGGGAAAGGAAA+CGG 0.559586 8.3:-22149679 MS.gene011716:CDS
GTAATGGTCGGGGTCATTCG+AGG 0.564407 8.3:-22149147 MS.gene011716:CDS
GTAAGTTGAACGATAACCAA+TGG 0.564502 8.3:+22149502 None:intergenic
TAATGAGTCTTTCAAAGTGG+TGG 0.571832 8.3:+22149738 None:intergenic
AAACACCAAGCGCGGTTGGT+TGG 0.571976 8.3:+22149062 None:intergenic
CGTTGATTGGAGGCGTCGTG+TGG 0.572282 8.3:-22149038 MS.gene011716:CDS
GGGAGTGTGTAATCAACTTG+TGG 0.575329 8.3:+22149538 None:intergenic
GCCGGTCGTTTACACGGGAA+AGG 0.576269 8.3:-22149685 MS.gene011716:CDS
TGCACTTCTCCAAATATGAC+CGG 0.576981 8.3:+22149111 None:intergenic
TTATCCGAATGGGATCAGAT+TGG 0.580343 8.3:-22149835 MS.gene011716:CDS
AGTTGTAGGGAATGGTGAGA+AGG 0.580345 8.3:-22148810 MS.gene011716:CDS
TTCAAGTAGGATAAGGGAAG+CGG 0.586112 8.3:-22148918 MS.gene011716:CDS
CCTTAGGGTATGCTTCAAGT+AGG 0.589064 8.3:-22148931 MS.gene011716:CDS
TGGTTCCATTGGTCTCACAT+AGG 0.592926 8.3:+22149879 None:intergenic
ATGATGAGTATGTTAGGTTG+GGG 0.599568 8.3:-22148883 MS.gene011716:CDS
GAGTGGGCTCTGATTGCATG+TGG 0.601269 8.3:-22149394 MS.gene011716:CDS
TTGGAATCCTAATTTAGGAG+TGG 0.605814 8.3:-22148777 MS.gene011716:CDS
AGTTGGAATAAACCTAGTAA+TGG 0.606296 8.3:-22149163 MS.gene011716:CDS
CAAGTGGATAAAACGGCACA+AGG 0.608604 8.3:+22149707 None:intergenic
TCAACATCAGTAGCATTCAA+AGG 0.614083 8.3:-22149907 MS.gene011716:CDS
ACTGATGTTGATGTTGAAGA+TGG 0.622854 8.3:+22149919 None:intergenic
GCTTAATGAGTCTTTCAAAG+TGG 0.631806 8.3:+22149735 None:intergenic
AGAAGTGCATGTAAAGCAAG+AGG 0.632435 8.3:-22149097 MS.gene011716:CDS
GGTAAACGACCGCACCATGT+TGG 0.632845 8.3:+22149859 None:intergenic
TTGTTCCTATGTGAGACCAA+TGG 0.634046 8.3:-22149884 MS.gene011716:CDS
TTGTAGGGAATGGTGAGAAG+GGG 0.646958 8.3:-22148808 MS.gene011716:CDS
AGTTGATTACACACTCCCGA+TGG 0.651878 8.3:-22149533 MS.gene011716:CDS
AATAAACCTAGTAATGGTCG+GGG 0.655287 8.3:-22149157 MS.gene011716:CDS
GAAGTGCATGTAAAGCAAGA+GGG 0.674901 8.3:-22149096 MS.gene011716:CDS
ATCAACGCAAACACCAAGCG+CGG 0.676455 8.3:+22149054 None:intergenic
CCAATGGAACCAACATGGTG+CGG 0.677814 8.3:-22149868 MS.gene011716:CDS
TAGTTCGGATAAAGTTAACG+AGG 0.728148 8.3:+22149603 None:intergenic
TGAGACCAATGGAACCAACA+TGG 0.751806 8.3:-22149873 MS.gene011716:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAGGATTCCAATAAAA+AGG + chr8.3:22149711-22149730 None:intergenic 20.0%
!!! CAACTTACAAATTTTAAATG+TGG - chr8.3:22149006-22149025 MS.gene011716:CDS 20.0%
!!! TTTTATTGGAATCCTAATTT+AGG - chr8.3:22149711-22149730 MS.gene011716:CDS 20.0%
! AGAAAGGAAGAAAAAAACAA+CGG - chr8.3:22149248-22149267 MS.gene011716:CDS 25.0%
! ATTCAGATAATGTTGAAGAA+AGG - chr8.3:22149232-22149251 MS.gene011716:CDS 25.0%
! TAACTGTAATATTCCAAAGA+AGG + chr8.3:22148928-22148947 None:intergenic 25.0%
! TCGTAACAATGTTAATGAAT+GGG - chr8.3:22149179-22149198 MS.gene011716:CDS 25.0%
! TCTGAATTTCCTAACAATAA+AGG + chr8.3:22149219-22149238 None:intergenic 25.0%
! TTGATAAAAATGCTCCTTAA+AGG + chr8.3:22149913-22149932 None:intergenic 25.0%
!! AAATACCCTTTTGGTAAATT+AGG + chr8.3:22149483-22149502 None:intergenic 25.0%
!! CAATTTATGGACTTGATTTT+GGG - chr8.3:22149757-22149776 MS.gene011716:CDS 25.0%
!! TTAACATTGTTACGACATTT+TGG + chr8.3:22149174-22149193 None:intergenic 25.0%
!!! AATTTACCAAAAGGGTATTT+TGG - chr8.3:22149483-22149502 MS.gene011716:CDS 25.0%
!!! ATTTACCAAAAGGGTATTTT+GGG - chr8.3:22149484-22149503 MS.gene011716:CDS 25.0%
AAGTGAATGATGAGTATGTT+AGG - chr8.3:22149604-22149623 MS.gene011716:CDS 30.0%
AATATAGAACCATCATCATC+GGG + chr8.3:22149855-22149874 None:intergenic 30.0%
AATGATGAGTATGTTAGGTT+GGG - chr8.3:22149609-22149628 MS.gene011716:CDS 30.0%
AGTTGGAATAAACCTAGTAA+TGG - chr8.3:22149330-22149349 MS.gene011716:CDS 30.0%
ATATAATGTCCAAACCCTTA+GGG - chr8.3:22149547-22149566 MS.gene011716:CDS 30.0%
ATGTTTGTATTGAGAAACCT+TGG + chr8.3:22149144-22149163 None:intergenic 30.0%
CAATATAGAACCATCATCAT+CGG + chr8.3:22149856-22149875 None:intergenic 30.0%
CCAAAATCAAGTCCATAAAT+TGG + chr8.3:22149759-22149778 None:intergenic 30.0%
GATCAACTTCCTTTATTGTT+AGG - chr8.3:22149207-22149226 MS.gene011716:CDS 30.0%
GTCGTAACAATGTTAATGAA+TGG - chr8.3:22149178-22149197 MS.gene011716:CDS 30.0%
TGGAACCTAATTTACCAAAA+GGG - chr8.3:22149475-22149494 MS.gene011716:CDS 30.0%
TTTATTCCAACTCTCATTTG+CGG + chr8.3:22149322-22149341 None:intergenic 30.0%
! AACAATAAAGGAAGTTGATC+AGG + chr8.3:22149207-22149226 None:intergenic 30.0%
! CCAATTTATGGACTTGATTT+TGG - chr8.3:22149756-22149775 MS.gene011716:CDS 30.0%
!!! GTTGCATTTCATTTTTGAGT+GGG - chr8.3:22149083-22149102 MS.gene011716:CDS 30.0%
AAGGAAGAAAAAAACAACGG+TGG - chr8.3:22149251-22149270 MS.gene011716:CDS 35.0%
AATAAACCTAGTAATGGTCG+GGG - chr8.3:22149336-22149355 MS.gene011716:CDS 35.0%
ACCAATGGAATATCTTCCAT+CGG + chr8.3:22148979-22148998 None:intergenic 35.0%
ACTGATGTTGATGTTGAAGA+TGG + chr8.3:22148577-22148596 None:intergenic 35.0%
AGTAGCCAAAACATCCAAAA+TGG + chr8.3:22149514-22149533 None:intergenic 35.0%
CAACTAACCACTCCTAAATT+AGG + chr8.3:22149726-22149745 None:intergenic 35.0%
CTAATTTAGGAGTGGTTAGT+TGG - chr8.3:22149724-22149743 MS.gene011716:CDS 35.0%
GAATAAACCTAGTAATGGTC+GGG - chr8.3:22149335-22149354 MS.gene011716:CDS 35.0%
GAATGATGAGTATGTTAGGT+TGG - chr8.3:22149608-22149627 MS.gene011716:CDS 35.0%
GATATAATGTCCAAACCCTT+AGG - chr8.3:22149546-22149565 MS.gene011716:CDS 35.0%
GCTTAATGAGTCTTTCAAAG+TGG + chr8.3:22148761-22148780 None:intergenic 35.0%
GGAATAAACCTAGTAATGGT+CGG - chr8.3:22149334-22149353 MS.gene011716:CDS 35.0%
GTATGCTTCAAGTAGGATAA+GGG - chr8.3:22149569-22149588 MS.gene011716:CDS 35.0%
GTGGAACCTAATTTACCAAA+AGG - chr8.3:22149474-22149493 MS.gene011716:CDS 35.0%
TAATGAGTCTTTCAAAGTGG+TGG + chr8.3:22148758-22148777 None:intergenic 35.0%
TAGTTCGGATAAAGTTAACG+AGG + chr8.3:22148893-22148912 None:intergenic 35.0%
TCAACATCAGTAGCATTCAA+AGG - chr8.3:22148586-22148605 MS.gene011716:CDS 35.0%
TCGTTAACTTTATCCGAACT+AGG - chr8.3:22148892-22148911 MS.gene011716:CDS 35.0%
TCGTTTACCATTATCCGAAT+GGG - chr8.3:22148648-22148667 MS.gene011716:CDS 35.0%
TGGTTGTCATTGCCAATTTA+TGG - chr8.3:22149744-22149763 MS.gene011716:CDS 35.0%
TGTAATCAACTTGTGGGATA+AGG + chr8.3:22148951-22148970 None:intergenic 35.0%
TTGGAATCCTAATTTAGGAG+TGG - chr8.3:22149716-22149735 MS.gene011716:CDS 35.0%
! ATGATGAGTATGTTAGGTTG+GGG - chr8.3:22149610-22149629 MS.gene011716:CDS 35.0%
! GGGTGGTAAAAGTATATTGT+TGG + chr8.3:22148700-22148719 None:intergenic 35.0%
! GTAAGTTGAACGATAACCAA+TGG + chr8.3:22148994-22149013 None:intergenic 35.0%
! TTTATGGACTTGATTTTGGG+TGG - chr8.3:22149760-22149779 MS.gene011716:CDS 35.0%
!! CGATGATGATGGTTCTATAT+TGG - chr8.3:22149854-22149873 MS.gene011716:CDS 35.0%
!! CGTTGCATTTCATTTTTGAG+TGG - chr8.3:22149082-22149101 MS.gene011716:CDS 35.0%
!! GTATTTTGGGAATGCCATTT+TGG - chr8.3:22149497-22149516 MS.gene011716:CDS 35.0%
!! TCCAAGATCTTCAAGTTGTA+GGG - chr8.3:22149670-22149689 MS.gene011716:CDS 35.0%
!! TTATGGACTTGATTTTGGGT+GGG - chr8.3:22149761-22149780 MS.gene011716:CDS 35.0%
!! TTCCAAGATCTTCAAGTTGT+AGG - chr8.3:22149669-22149688 MS.gene011716:CDS 35.0%
!!! GAATGCCATTTTGGATGTTT+TGG - chr8.3:22149506-22149525 MS.gene011716:CDS 35.0%
AAAATGAAATGCAACGCGCT+TGG + chr8.3:22149078-22149097 None:intergenic 40.0%
AAGCAGTAACCGGTCATATT+TGG - chr8.3:22149373-22149392 MS.gene011716:CDS 40.0%
AGGTGAAAAGTCACCTAGTT+CGG + chr8.3:22148908-22148927 None:intergenic 40.0%
ATCTGATCCCATTCGGATAA+TGG + chr8.3:22148658-22148677 None:intergenic 40.0%
CCAATGGAATATCTTCCATC+GGG + chr8.3:22148978-22148997 None:intergenic 40.0%
CTACTTGAAGCATACCCTAA+GGG + chr8.3:22149564-22149583 None:intergenic 40.0%
GAAAAACCGCAAATGAGAGT+TGG - chr8.3:22149313-22149332 MS.gene011716:CDS 40.0%
GAAGTGCATGTAAAGCAAGA+GGG - chr8.3:22149397-22149416 MS.gene011716:CDS 40.0%
GATCCATATGGATGCCTTTA+AGG - chr8.3:22149896-22149915 MS.gene011716:CDS 40.0%
GATCTCGAATGCAACTCATT+GGG - chr8.3:22148844-22148863 MS.gene011716:CDS 40.0%
GATTCCAATCTGATCCCATT+CGG + chr8.3:22148665-22148684 None:intergenic 40.0%
GGAACACATGAAGAACTTGA+TGG - chr8.3:22149807-22149826 MS.gene011716:CDS 40.0%
GGAGTGTGTAATCAACTTGT+GGG + chr8.3:22148957-22148976 None:intergenic 40.0%
GGTATGCTTCAAGTAGGATA+AGG - chr8.3:22149568-22149587 MS.gene011716:CDS 40.0%
GTCGTTTACCATTATCCGAA+TGG - chr8.3:22148647-22148666 MS.gene011716:CDS 40.0%
TCCCTACAACTTGAAGATCT+TGG + chr8.3:22149674-22149693 None:intergenic 40.0%
TGATCTCGAATGCAACTCAT+TGG - chr8.3:22148843-22148862 MS.gene011716:CDS 40.0%
TGCACTTCTCCAAATATGAC+CGG + chr8.3:22149385-22149404 None:intergenic 40.0%
TTATCCGAATGGGATCAGAT+TGG - chr8.3:22148658-22148677 MS.gene011716:CDS 40.0%
TTCAAGTAGGATAAGGGAAG+CGG - chr8.3:22149575-22149594 MS.gene011716:CDS 40.0%
TTGTCTGCAAGAGATCCATA+TGG - chr8.3:22149884-22149903 MS.gene011716:CDS 40.0%
TTGTTCCTATGTGAGACCAA+TGG - chr8.3:22148609-22148628 MS.gene011716:CDS 40.0%
! AACCTTGGCACCATTTTCAA+AGG + chr8.3:22149129-22149148 None:intergenic 40.0%
! AGAAGTGCATGTAAAGCAAG+AGG - chr8.3:22149396-22149415 MS.gene011716:CDS 40.0%
! AGGTGACTTTTCACCTTCTT+TGG - chr8.3:22148912-22148931 MS.gene011716:CDS 40.0%
! ATGTGGTGAGCCTTTGAAAA+TGG - chr8.3:22149116-22149135 MS.gene011716:CDS 40.0%
! GCATTCCCAAAATACCCTTT+TGG + chr8.3:22149492-22149511 None:intergenic 40.0%
!! AAAGTGGTGGCGATTTTAGT+GGG + chr8.3:22148745-22148764 None:intergenic 40.0%
!! GATCTTCAAGTTGTAGGGAA+TGG - chr8.3:22149675-22149694 MS.gene011716:CDS 40.0%
!! TATGGACTTGATTTTGGGTG+GGG - chr8.3:22149762-22149781 MS.gene011716:CDS 40.0%
!!! TGTTTTGGCTACTAGTCTAG+CGG - chr8.3:22149521-22149540 MS.gene011716:CDS 40.0%
AAGGAGGTTTATATGGGGCT+AGG - chr8.3:22149786-22149805 MS.gene011716:CDS 45.0%
AGTTGATTACACACTCCCGA+TGG - chr8.3:22148960-22148979 MS.gene011716:CDS 45.0%
AGTTGTAGGGAATGGTGAGA+AGG - chr8.3:22149683-22149702 MS.gene011716:CDS 45.0%
ATCTCACATGCAGTTGCAGA+TGG - chr8.3:22149051-22149070 MS.gene011716:CDS 45.0%
CAAGTGGATAAAACGGCACA+AGG + chr8.3:22148789-22148808 None:intergenic 45.0%
CCCGATGGAAGATATTCCAT+TGG - chr8.3:22148975-22148994 MS.gene011716:CDS 45.0%
CCTACTTGAAGCATACCCTA+AGG + chr8.3:22149565-22149584 None:intergenic 45.0%
CCTTAGGGTATGCTTCAAGT+AGG - chr8.3:22149562-22149581 MS.gene011716:CDS 45.0%
CGTTTACACGGGAAAGGAAA+CGG - chr8.3:22148814-22148833 MS.gene011716:CDS 45.0%
GTTGTAGGGAATGGTGAGAA+GGG - chr8.3:22149684-22149703 MS.gene011716:CDS 45.0%
TCGAGGTATGAAGCAGTAAC+CGG - chr8.3:22149363-22149382 MS.gene011716:CDS 45.0%
TGAAGCATACCCTAAGGGTT+TGG + chr8.3:22149559-22149578 None:intergenic 45.0%
TGAGACCAATGGAACCAACA+TGG - chr8.3:22148620-22148639 MS.gene011716:CDS 45.0%
TGGGGGAAGGAGGTTTATAT+GGG - chr8.3:22149780-22149799 MS.gene011716:CDS 45.0%
TTGTAGGGAATGGTGAGAAG+GGG - chr8.3:22149685-22149704 MS.gene011716:CDS 45.0%
! AGCCTTTGAAAATGGTGCCA+AGG - chr8.3:22149124-22149143 MS.gene011716:CDS 45.0%
! GGGAGTGTGTAATCAACTTG+TGG + chr8.3:22148958-22148977 None:intergenic 45.0%
! GTGAGAAGGGGCCTTTTTAT+TGG - chr8.3:22149697-22149716 MS.gene011716:CDS 45.0%
! TGGTTCCATTGGTCTCACAT+AGG + chr8.3:22148617-22148636 None:intergenic 45.0%
!! AAGTGGTGGCGATTTTAGTG+GGG + chr8.3:22148744-22148763 None:intergenic 45.0%
!! ATGGACTTGATTTTGGGTGG+GGG - chr8.3:22149763-22149782 MS.gene011716:CDS 45.0%
!! CAAAGTGGTGGCGATTTTAG+TGG + chr8.3:22148746-22148765 None:intergenic 45.0%
!! GCTCCTTAAAGGCATCCATA+TGG + chr8.3:22149902-22149921 None:intergenic 45.0%
!! TTGGTGTTTGCGTTGATTGG+AGG - chr8.3:22149445-22149464 MS.gene011716:CDS 45.0%
!!! GTTTTGGCTACTAGTCTAGC+GGG - chr8.3:22149522-22149541 MS.gene011716:CDS 45.0%
ATCAACGCAAACACCAAGCG+CGG + chr8.3:22149442-22149461 None:intergenic 50.0%
CCAATGGAACCAACATGGTG+CGG - chr8.3:22148625-22148644 MS.gene011716:CDS 50.0%
CGACCGGCAAGTGGATAAAA+CGG + chr8.3:22148796-22148815 None:intergenic 50.0%
CTTCCTAGTCCCGATGATGA+TGG - chr8.3:22149843-22149862 MS.gene011716:CDS 50.0%
GAACCATCATCATCGGGACT+AGG + chr8.3:22149849-22149868 None:intergenic 50.0%
GAATGACCCCGACCATTACT+AGG + chr8.3:22149345-22149364 None:intergenic 50.0%
GGGGGAAGGAGGTTTATATG+GGG - chr8.3:22149781-22149800 MS.gene011716:CDS 50.0%
GTGGGGGAAGGAGGTTTATA+TGG - chr8.3:22149779-22149798 MS.gene011716:CDS 50.0%
TCCTTTCCCGTGTAAACGAC+CGG + chr8.3:22148812-22148831 None:intergenic 50.0%
TTTACCACCCCCCAAAAAGC+TGG - chr8.3:22148710-22148729 MS.gene011716:CDS 50.0%
! GAGGTAAGCCAGCTTTTTGG+GGG + chr8.3:22148721-22148740 None:intergenic 50.0%
! GGAGGTAAGCCAGCTTTTTG+GGG + chr8.3:22148722-22148741 None:intergenic 50.0%
! GGGAGGTAAGCCAGCTTTTT+GGG + chr8.3:22148723-22148742 None:intergenic 50.0%
!! ACTTGATTTTGGGTGGGGGA+AGG - chr8.3:22149767-22149786 MS.gene011716:CDS 50.0%
!! AGGTAAGCCAGCTTTTTGGG+GGG + chr8.3:22148720-22148739 None:intergenic 50.0%
!! AGTGGTGGCGATTTTAGTGG+GGG + chr8.3:22148743-22148762 None:intergenic 50.0%
!! CGCTTGGTGTTTGCGTTGAT+TGG - chr8.3:22149442-22149461 MS.gene011716:CDS 50.0%
!!! GTGCCGTTTTATCCACTTGC+CGG - chr8.3:22148790-22148809 MS.gene011716:CDS 50.0%
AAACACCAAGCGCGGTTGGT+TGG + chr8.3:22149434-22149453 None:intergenic 55.0%
AATGACCAACCAACCGCGCT+TGG - chr8.3:22149426-22149445 MS.gene011716:CDS 55.0%
CACTTGCCGGTCGTTTACAC+GGG - chr8.3:22148803-22148822 MS.gene011716:CDS 55.0%
CCACTTGCCGGTCGTTTACA+CGG - chr8.3:22148802-22148821 MS.gene011716:CDS 55.0%
GGTAAACGACCGCACCATGT+TGG + chr8.3:22148637-22148656 None:intergenic 55.0%
GTAATGGTCGGGGTCATTCG+AGG - chr8.3:22149346-22149365 MS.gene011716:CDS 55.0%
! GAGTGGGCTCTGATTGCATG+TGG - chr8.3:22149099-22149118 MS.gene011716:CDS 55.0%
! GGGGAGGTAAGCCAGCTTTT+TGG + chr8.3:22148724-22148743 None:intergenic 55.0%
!! CCGCACCATGTTGGTTCCAT+TGG + chr8.3:22148628-22148647 None:intergenic 55.0%
!! TAAGCCAGCTTTTTGGGGGG+TGG + chr8.3:22148717-22148736 None:intergenic 55.0%
!! TGATTTTGGGTGGGGGAAGG+AGG - chr8.3:22149770-22149789 MS.gene011716:CDS 55.0%
ACGCAAACACCAAGCGCGGT+TGG + chr8.3:22149438-22149457 None:intergenic 60.0%
CCGTGTAAACGACCGGCAAG+TGG + chr8.3:22148805-22148824 None:intergenic 60.0%
GCCGGTCGTTTACACGGGAA+AGG - chr8.3:22148808-22148827 MS.gene011716:CDS 60.0%
!! CGTTGATTGGAGGCGTCGTG+TGG - chr8.3:22149455-22149474 MS.gene011716:CDS 60.0%
!! GGTGGCGATTTTAGTGGGGG+AGG + chr8.3:22148740-22148759 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 22148571 22149944 22148571 ID=MS.gene011716
chr8.3 mRNA 22148571 22149944 22148571 ID=MS.gene011716.t1;Parent=MS.gene011716
chr8.3 exon 22148571 22149944 22148571 ID=MS.gene011716.t1.exon1;Parent=MS.gene011716.t1
chr8.3 CDS 22148571 22149944 22148571 ID=cds.MS.gene011716.t1;Parent=MS.gene011716.t1
Gene Sequence

>MS.gene011716

ATGCCATCTTCAACATCAACATCAGTAGCATTCAAAGGTTGTTCCTATGTGAGACCAATGGAACCAACATGGTGCGGTCGTTTACCATTATCCGAATGGGATCAGATTGGAATCATAGCACACGTGCCAACAATATACTTTTACCACCCCCCAAAAAGCTGGCTTACCTCCCCCACTAAAATCGCCACCACTTTGAAAGACTCATTAAGCAAAGTCCTTGTGCCGTTTTATCCACTTGCCGGTCGTTTACACGGGAAAGGAAACGGTCGTTTTGATCTCGAATGCAACTCATTGGGTGTTCAATTCATCGAAGCACAATCCTCGTTAACTTTATCCGAACTAGGTGACTTTTCACCTTCTTTGGAATATTACAGTTACCTTATCCCACAAGTTGATTACACACTCCCGATGGAAGATATTCCATTGGTTATCGTTCAACTTACAAATTTTAAATGTGGTGTAGTTAGTATTAGTTTGTTAATCTCACATGCAGTTGCAGATGGACCAAGCGCGTTGCATTTCATTTTTGAGTGGGCTCTGATTGCATGTGGTGAGCCTTTGAAAATGGTGCCAAGGTTTCTCAATACAAACATGTTGTCGCCAAAATGTCGTAACAATGTTAATGAATGGGAGCCTGATCAACTTCCTTTATTGTTAGGAAATTCAGATAATGTTGAAGAAAGGAAGAAAAAAACAACGGTGGCTATAATCAACTTGAGCAAAACACAAGTTGAAAAGTTGAGAAAAACCGCAAATGAGAGTTGGAATAAACCTAGTAATGGTCGGGGTCATTCGAGGTATGAAGCAGTAACCGGTCATATTTGGAGAAGTGCATGTAAAGCAAGAGGGCTTGAAAATGACCAACCAACCGCGCTTGGTGTTTGCGTTGATTGGAGGCGTCGTGTGGAACCTAATTTACCAAAAGGGTATTTTGGGAATGCCATTTTGGATGTTTTGGCTACTAGTCTAGCGGGTGATATAATGTCCAAACCCTTAGGGTATGCTTCAAGTAGGATAAGGGAAGCGGTTGAGAAAGTGAATGATGAGTATGTTAGGTTGGGGATTGAGTACTTTAAGAAGCAAGAAGATTTGACTAAGTTCCAAGATCTTCAAGTTGTAGGGAATGGTGAGAAGGGGCCTTTTTATTGGAATCCTAATTTAGGAGTGGTTAGTTGGTTGTCATTGCCAATTTATGGACTTGATTTTGGGTGGGGGAAGGAGGTTTATATGGGGCTAGGAACACATGAAGAACTTGATGGAGACTCATTGCTTCTTCCTAGTCCCGATGATGATGGTTCTATATTGGTTATTATTTGTCTGCAAGAGATCCATATGGATGCCTTTAAGGAGCATTTTTATCAAGACATTGTGTGA
Protein sequence

>MS.gene011716.t1

MPSSTSTSVAFKGCSYVRPMEPTWCGRLPLSEWDQIGIIAHVPTIYFYHPPKSWLTSPTKIATTLKDSLSKVLVPFYPLAGRLHGKGNGRFDLECNSLGVQFIEAQSSLTLSELGDFSPSLEYYSYLIPQVDYTLPMEDIPLVIVQLTNFKCGVVSISLLISHAVADGPSALHFIFEWALIACGEPLKMVPRFLNTNMLSPKCRNNVNEWEPDQLPLLLGNSDNVEERKKKTTVAIINLSKTQVEKLRKTANESWNKPSNGRGHSRYEAVTGHIWRSACKARGLENDQPTALGVCVDWRRRVEPNLPKGYFGNAILDVLATSLAGDIMSKPLGYASSRIREAVEKVNDEYVRLGIEYFKKQEDLTKFQDLQVVGNGEKGPFYWNPNLGVVSWLSLPIYGLDFGWGKEVYMGLGTHEELDGDSLLLPSPDDDGSILVIICLQEIHMDAFKEHFYQDIV