AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene039174

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039174.t1 MTR_2g070130 93.607 219 14 0 1 219 1 219 2.34e-152 422
MS.gene039174.t1 MTR_2g070150 85.784 204 29 0 1 204 1 204 1.95e-132 370
MS.gene039174.t1 MTR_2g070180 81.308 214 40 0 1 214 1 214 2.78e-131 368
MS.gene039174.t1 MTR_2g070140 81.773 203 37 0 17 219 1 203 5.84e-127 357
MS.gene039174.t1 MTR_2g070210 70.136 221 64 1 1 219 1 221 1.45e-116 331
MS.gene039174.t1 MTR_2g070200 68.326 221 68 2 1 219 1 221 3.05e-110 315
MS.gene039174.t1 MTR_2g070060 60.731 219 86 0 1 219 1 219 2.68e-101 292
MS.gene039174.t1 MTR_2g070070 61.187 219 85 0 1 219 1 219 9.92e-100 288
MS.gene039174.t1 MTR_3g467420 61.860 215 82 0 5 219 10 224 1.67e-99 288
MS.gene039174.t1 MTR_8g056940 61.111 216 84 0 1 216 1 216 3.76e-96 280
MS.gene039174.t1 MTR_3g467430 59.447 217 88 0 3 219 8 224 4.71e-96 279
MS.gene039174.t1 MTR_2g070110 60.274 219 86 1 1 219 1 218 6.58e-94 274
MS.gene039174.t1 MTR_2g070120 60.190 211 84 0 1 211 1 211 1.60e-93 273
MS.gene039174.t1 MTR_4g124130 57.727 220 92 1 1 219 1 220 3.51e-91 267
MS.gene039174.t1 MTR_5g076900 49.315 219 109 2 2 219 4 221 3.61e-74 224
MS.gene039174.t1 MTR_0186s0030 68.794 141 44 0 1 141 1 141 9.18e-66 199
MS.gene039174.t1 MTR_1g090060 42.718 206 113 3 4 205 3 207 1.37e-51 166
MS.gene039174.t1 MTR_7g065720 43.602 211 105 5 3 205 6 210 2.25e-51 166
MS.gene039174.t1 MTR_8g087425 43.204 206 114 2 2 205 3 207 2.96e-51 166
MS.gene039174.t1 MTR_4g019780 43.415 205 113 2 3 205 7 210 7.53e-51 164
MS.gene039174.t1 MTR_7g065750 44.144 222 107 6 3 216 6 218 1.50e-49 161
MS.gene039174.t1 MTR_4g059730 41.627 209 117 3 1 205 1 208 3.80e-49 160
MS.gene039174.t1 MTR_4g019790 44.390 205 109 5 4 205 7 209 9.83e-49 159
MS.gene039174.t1 MTR_3g099757 42.439 205 115 3 3 205 4 207 1.45e-48 159
MS.gene039174.t1 MTR_7g065740 43.415 205 110 4 3 203 6 208 1.70e-48 159
MS.gene039174.t1 MTR_5g037380 44.601 213 109 6 2 205 3 215 2.84e-48 158
MS.gene039174.t1 MTR_7g065630 42.927 205 108 5 3 204 24 222 3.89e-48 158
MS.gene039174.t1 MTR_5g040430 37.668 223 129 3 2 217 5 224 1.01e-47 156
MS.gene039174.t1 MTR_8g087410 42.157 204 115 2 4 205 6 208 1.21e-47 156
MS.gene039174.t1 MTR_7g065660 41.748 206 115 4 3 205 6 209 3.90e-47 155
MS.gene039174.t1 MTR_7g065710 42.512 207 110 6 1 204 1 201 1.31e-46 154
MS.gene039174.t1 MTR_7g065270 43.137 204 111 5 3 203 6 207 1.42e-46 154
MS.gene039174.t1 MTR_6g080440 42.408 191 105 4 18 205 17 205 3.07e-46 152
MS.gene039174.t1 MTR_7g065290 43.204 206 112 5 3 205 6 209 9.02e-46 151
MS.gene039174.t1 MTR_1g090070 41.262 206 115 5 4 204 9 213 1.58e-45 151
MS.gene039174.t1 MTR_7g065265 42.718 206 113 5 3 205 6 209 4.42e-45 150
MS.gene039174.t1 MTR_1g115195 42.180 211 113 6 1 205 1 208 4.52e-45 150
MS.gene039174.t1 MTR_1g090100 41.463 205 115 3 5 205 3 206 8.20e-45 149
MS.gene039174.t1 MTR_1g090090 41.395 215 118 5 4 211 3 216 1.00e-44 149
MS.gene039174.t1 MTR_7g065230 43.902 205 103 6 3 203 6 202 1.56e-44 148
MS.gene039174.t1 MTR_7g065590 39.806 206 119 4 3 205 6 209 1.58e-44 148
MS.gene039174.t1 MTR_1g090150 37.557 221 120 7 3 215 2 212 1.02e-43 146
MS.gene039174.t1 MTR_7g065700 44.498 209 107 6 1 206 4 206 9.20e-43 144
MS.gene039174.t1 MTR_7g065680 50.920 163 75 4 3 162 6 166 9.72e-43 144
MS.gene039174.t1 MTR_8g061950 37.668 223 130 3 3 216 4 226 1.53e-41 141
MS.gene039174.t1 MTR_7g065600 37.788 217 127 5 3 216 6 217 2.63e-41 140
MS.gene039174.t1 MTR_7g065640 41.379 203 110 5 3 197 6 207 3.34e-41 139
MS.gene039174.t1 MTR_7g065260 41.176 187 105 5 3 186 13 197 1.70e-36 128
MS.gene039174.t1 MTR_2g072120 69.118 68 21 0 47 114 1 68 5.62e-30 106
MS.gene039174.t1 MTR_1g067180 27.941 204 134 6 11 209 31 226 5.23e-11 60.5
MS.gene039174.t1 MTR_1g067180 28.431 204 131 7 11 208 34 228 9.45e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene039174.t1 AT1G17180 64.840 219 77 0 1 219 1 219 1.45e-106 306
MS.gene039174.t1 AT1G78380 64.352 216 77 0 1 216 1 216 2.46e-104 300
MS.gene039174.t1 AT1G78340 63.426 216 79 0 1 216 1 216 6.82e-102 294
MS.gene039174.t1 AT1G78360 62.727 220 81 1 1 219 1 220 6.24e-101 291
MS.gene039174.t1 AT1G78370 63.084 214 79 0 1 214 1 214 1.03e-100 291
MS.gene039174.t1 AT1G78320 65.854 205 70 0 1 205 1 205 3.88e-100 290
MS.gene039174.t1 AT1G17170 61.644 219 83 1 1 219 1 218 1.31e-98 286
MS.gene039174.t1 AT1G17190 57.209 215 92 0 3 217 4 218 6.75e-94 274
MS.gene039174.t1 AT1G53680 54.378 217 97 2 5 219 8 224 3.69e-86 254
MS.gene039174.t1 AT3G43800 55.455 220 94 3 3 218 4 223 1.00e-81 243
MS.gene039174.t1 AT1G78320 56.098 205 63 1 1 205 1 178 1.52e-77 231
MS.gene039174.t1 AT2G29420 43.415 205 113 3 2 204 7 210 1.36e-56 179
MS.gene039174.t1 AT3G09270 44.037 218 116 4 3 217 5 219 7.13e-55 175
MS.gene039174.t1 AT2G29490 42.233 206 113 4 3 204 6 209 9.03e-53 169
MS.gene039174.t1 AT2G29480 41.262 206 115 4 3 204 6 209 6.75e-51 165
MS.gene039174.t1 AT2G29460 41.262 206 115 4 3 204 6 209 1.30e-50 164
MS.gene039174.t1 AT2G29440 41.262 206 115 4 3 204 5 208 1.32e-50 164
MS.gene039174.t1 AT2G29450 41.063 207 115 5 3 204 5 209 6.67e-50 162
MS.gene039174.t1 AT2G29470 40.000 205 116 5 5 204 8 210 5.17e-48 157
MS.gene039174.t1 AT1G59700 41.364 220 120 5 3 213 5 224 8.40e-48 157
MS.gene039174.t1 AT1G74590 39.091 220 124 5 3 216 6 221 3.49e-46 153
MS.gene039174.t1 AT1G10360 40.639 219 122 4 2 212 3 221 1.71e-45 151
MS.gene039174.t1 AT1G69930 39.423 208 119 3 5 205 13 220 2.22e-44 148
MS.gene039174.t1 AT1G10370 40.741 216 118 5 2 208 3 217 3.41e-44 147
MS.gene039174.t1 AT1G59670 40.359 223 122 5 3 215 5 226 6.84e-44 147
MS.gene039174.t1 AT1G27140 39.631 217 121 4 3 209 5 221 1.02e-43 147
MS.gene039174.t1 AT1G27130 38.208 212 122 4 3 205 5 216 6.54e-42 142
MS.gene039174.t1 AT5G62480 35.909 220 129 4 1 209 5 223 7.19e-42 142
MS.gene039174.t1 AT1G69920 36.607 224 124 6 5 216 35 252 1.84e-38 134
MS.gene039174.t1 AT5G62480 29.817 218 114 5 3 209 7 196 6.10e-25 97.8
MS.gene039174.t1 AT5G62480 29.817 218 115 5 3 209 7 197 6.30e-25 97.8

Find 48 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTAAAGATTCACAAAT+TGG 0.245931 2.1:-28437403 None:intergenic
ATCCCTGTTCTTATTCATAA+TGG 0.265063 2.1:+28437376 MS.gene039174:CDS
CCATTATACAATTGGTTTAG+AGG 0.271694 2.1:+28439475 MS.gene039174:CDS
TTCAGTATATTGATGAAGTT+TGG 0.281776 2.1:+28437428 MS.gene039174:CDS
AAGTGATTCTGCTTAATTAC+TGG 0.295778 2.1:+28437227 MS.gene039174:CDS
TTCAATCTGCATGCATCTAT+TGG 0.310100 2.1:-28439557 None:intergenic
AATTACTGGCCAAGTCCTTT+TGG 0.329731 2.1:+28437241 MS.gene039174:CDS
CTAGCTTGTGCTTTCTGATA+AGG 0.347248 2.1:-28437481 None:intergenic
CCTCTAAACCAATTGTATAA+TGG 0.353655 2.1:-28439475 None:intergenic
CATTTATTCCATTATACAAT+TGG 0.359711 2.1:+28439467 MS.gene039174:CDS
CAAAATAGCCCTAGCTGAAA+AGG 0.359899 2.1:+28437273 MS.gene039174:CDS
TTTCCATTATGAATAAGAAC+AGG 0.384624 2.1:-28437379 None:intergenic
TCATGGATCTTATCCTGATC+AGG 0.386076 2.1:-28439598 None:intergenic
AAATAGATCTATGAAGTTGC+AGG 0.398837 2.1:+28439310 MS.gene039174:intron
AGAAAGCACAAGCTAGATTC+TGG 0.400529 2.1:+28437488 MS.gene039174:CDS
ATGAAGTTGCAGGGAACAAT+TGG 0.408328 2.1:+28439320 MS.gene039174:CDS
GTGGAGCTCAAGAAGAAGTA+TGG 0.423879 2.1:+28439628 MS.gene039174:CDS
TTGGCCCAAGCACTGAACTT+AGG 0.425895 2.1:-28439538 None:intergenic
TTTAGAGGCTATGAGGCCTT+TGG 0.433110 2.1:+28439490 MS.gene039174:CDS
ACTCTTGCTCCAAGAGTTTG+AGG 0.435255 2.1:-28439393 None:intergenic
AGTCTCATGCCAAAAGGACT+TGG 0.442560 2.1:-28437250 None:intergenic
CATAGAAGCCCTCAAACTCT+TGG 0.450864 2.1:+28439384 MS.gene039174:CDS
TTCCAAGTCACTCCCTGATC+AGG 0.451766 2.1:+28439585 MS.gene039174:CDS
TGGCCCAAGCACTGAACTTA+GGG 0.452366 2.1:-28439537 None:intergenic
GGTTCATTTGTAACAACAAA+GGG 0.474781 2.1:-28437339 None:intergenic
GTGCCCTAAGTTCAGTGCTT+GGG 0.482104 2.1:+28439534 MS.gene039174:CDS
CTCTTGCTCCAAGAGTTTGA+GGG 0.482617 2.1:-28439392 None:intergenic
GAAAGCACAAGCTAGATTCT+GGG 0.487661 2.1:+28437489 MS.gene039174:CDS
CAAACTCTTGGAGCAAGAGT+TGG 0.489749 2.1:+28439396 MS.gene039174:CDS
AAACTCTTGGAGCAAGAGTT+GGG 0.491382 2.1:+28439397 MS.gene039174:CDS
AGAGCAAGAAGCTGCAAAGA+AGG 0.498696 2.1:+28439357 MS.gene039174:CDS
GGCTGATTATATTGATAAGA+AGG 0.507102 2.1:+28437510 MS.gene039174:CDS
CAATTGGTTTAGAGGCTATG+AGG 0.508218 2.1:+28439483 MS.gene039174:CDS
AAAATAGCCCTAGCTGAAAA+GGG 0.509162 2.1:+28437274 MS.gene039174:CDS
AGTGCCCTAAGTTCAGTGCT+TGG 0.527093 2.1:+28439533 MS.gene039174:CDS
TGAGCTCCACAATCAAATCA+TGG 0.537535 2.1:-28439615 None:intergenic
TAAGATCCATGATTTGATTG+TGG 0.557919 2.1:+28439609 MS.gene039174:CDS
CATGGATCTTATCCTGATCA+GGG 0.576720 2.1:-28439597 None:intergenic
GAAGAAGTATGGCTTTGAGT+AGG 0.577171 2.1:+28439639 MS.gene039174:CDS
GCTTTCTGATAAGGATCAGA+AGG 0.581675 2.1:-28437472 None:intergenic
CAGAATCACTTCATCAGCCA+TGG 0.586532 2.1:-28437215 None:intergenic
AATAGATCTATGAAGTTGCA+GGG 0.596469 2.1:+28439311 MS.gene039174:intron
GGGTTCATTTGTAACAACAA+AGG 0.598423 2.1:-28437340 None:intergenic
GTCTACACTGATCTTGCCAA+AGG 0.602126 2.1:-28439506 None:intergenic
TGGCAAGATCAGTGTAGACA+AGG 0.634237 2.1:+28439510 MS.gene039174:CDS
TTCCATTATGAATAAGAACA+GGG 0.638163 2.1:-28437378 None:intergenic
ATCCTGATCAGGGAGTGACT+TGG 0.640797 2.1:-28439587 None:intergenic
TAAGGATCAGAAGGTAACAA+AGG 0.642669 2.1:-28437463 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAATATTAAAAAATTT+TGG - chr2.1:28438865-28438884 None:intergenic 0.0%
!! TATATAAAAAAAAATTTGCA+GGG - chr2.1:28437903-28437922 None:intergenic 10.0%
!!! ATTTTTTTATTATATAACTG+GGG + chr2.1:28439055-28439074 MS.gene039174:intron 10.0%
!!! CAAAATTTTAATAAAAATTC+TGG + chr2.1:28438352-28438371 MS.gene039174:intron 10.0%
!!! CAAATTTTTTTTATAGTTTT+TGG - chr2.1:28437863-28437844 None:intergenic 10.0%
!!! CAATTTTTTTTTATAGTTTT+TGG + chr2.1:28437784-28437803 MS.gene039174:intron 10.0%
!!! TTCTAAAAAATTAAAAATTC+TGG + chr2.1:28438131-28438150 MS.gene039174:intron 10.0%
!! AAACTATAAAAAAAAATTGC+AGG - chr2.1:28437783-28437802 None:intergenic 15.0%
!! AAACTATAAAAAAAATTTGC+AGG + chr2.1:28439151-28439170 MS.gene039174:intron 15.0%
!! AAACTATAAAAAAAATTTGC+AGG - chr2.1:28439170-28439151 None:intergenic 15.0%
!! AACTATAAAAAAAAATTGCA+GGG - chr2.1:28437782-28437801 None:intergenic 15.0%
!! AACTATAAAAAAAATTTGCA+GGG + chr2.1:28439152-28439171 MS.gene039174:intron 15.0%
!! AACTATAAAAAAAATTTGCA+GGG - chr2.1:28439171-28439152 None:intergenic 15.0%
!! AGGAAAAAATTTAAAAATTC+TGG - chr2.1:28438796-28438815 None:intergenic 15.0%
!! CTATATAAAAAAAAATTTGC+AGG - chr2.1:28437904-28437923 None:intergenic 15.0%
!! TATAAATACACAAAAATTCT+TGG + chr2.1:28438406-28438425 MS.gene039174:intron 15.0%
!! TCTGAAAAAAATTAAAATTC+TGG - chr2.1:28438650-28438669 None:intergenic 15.0%
!! TTTATTTCCAAAAAAATTTC+CGG - chr2.1:28438545-28438564 None:intergenic 15.0%
!!! ATTTTTGAGATAATTCATAT+TGG + chr2.1:28437659-28437678 MS.gene039174:intron 15.0%
!!! ATTTTTGTGTATTTATAAAC+CGG - chr2.1:28438403-28438422 None:intergenic 15.0%
!!! GGAAATTTTAATAAAAATTC+TGG - chr2.1:28438613-28438632 None:intergenic 15.0%
!!! TTTTTGAGATAATTCATATT+GGG + chr2.1:28437660-28437679 MS.gene039174:intron 15.0%
!! CATTTATTCCATTATACAAT+TGG + chr2.1:28439467-28439486 MS.gene039174:CDS 20.0%
!! TATTTATAAACCGAGAATAA+GGG - chr2.1:28438512-28438531 None:intergenic 20.0%
!! TTATTTCCAAAAAAATTTCC+GGG - chr2.1:28438544-28438563 None:intergenic 20.0%
!!! ATTCTGGAAAAATGAAATTT+TGG - chr2.1:28438634-28438653 None:intergenic 20.0%
!!! ATTTTTCCAGAATTTTTGTA+GGG - chr2.1:28437935-28437954 None:intergenic 20.0%
!!! TTTTTGTGTATTTATAAACC+GGG - chr2.1:28438402-28438421 None:intergenic 20.0%
! AATAGGCTTAAATATGGTAT+TGG - chr2.1:28439210-28439229 None:intergenic 25.0%
! CTGGGAAAATAAAAAAATTC+TGG - chr2.1:28439014-28439033 None:intergenic 25.0%
! GCAATTAAAGATTCACAAAT+TGG - chr2.1:28437406-28437425 None:intergenic 25.0%
! GCATGCTAAAAATATCAAAT+GGG - chr2.1:28437690-28437709 None:intergenic 25.0%
! GTATTTATAAACCGAGAATA+AGG - chr2.1:28438513-28438532 None:intergenic 25.0%
! TATTAGCAAAGGCTTAAATA+TGG + chr2.1:28437718-28437737 MS.gene039174:intron 25.0%
! TGCATAATCATTATTAGCAA+AGG + chr2.1:28437707-28437726 MS.gene039174:intron 25.0%
! TGCATGCTAAAAATATCAAA+TGG - chr2.1:28437691-28437710 None:intergenic 25.0%
! TTATAAATACGCAAAATTCC+CGG + chr2.1:28438523-28438542 MS.gene039174:intron 25.0%
! TTCAGTATATTGATGAAGTT+TGG + chr2.1:28437428-28437447 MS.gene039174:CDS 25.0%
! TTCCATTATGAATAAGAACA+GGG - chr2.1:28437381-28437400 None:intergenic 25.0%
! TTTCCATTATGAATAAGAAC+AGG - chr2.1:28437382-28437401 None:intergenic 25.0%
! TTTGCTAATAGGCTTAAATA+TGG - chr2.1:28439216-28439235 None:intergenic 25.0%
!! CATTTTTCCAGAATTTTTGT+AGG - chr2.1:28437936-28437955 None:intergenic 25.0%
!! CCAAAAACAAAAATTTTGCA+GGG + chr2.1:28439091-28439110 MS.gene039174:intron 25.0%
!! GTATCATGTTATTTGCTAAT+AGG - chr2.1:28439227-28439246 None:intergenic 25.0%
!! TCCAAAAACAAAAATTTTGC+AGG + chr2.1:28439090-28439109 MS.gene039174:intron 25.0%
!! TTTCTGAAAAGTTTAGACAT+TGG + chr2.1:28437588-28437607 MS.gene039174:intron 25.0%
!!! AAAATTCCCGGAAATTTTTT+TGG + chr2.1:28438535-28438554 MS.gene039174:intron 25.0%
!!! CATATTTTTTCCCTTATTCT+CGG + chr2.1:28438499-28438518 MS.gene039174:intron 25.0%
AAATAGATCTATGAAGTTGC+AGG + chr2.1:28439310-28439329 MS.gene039174:intron 30.0%
AAGTGATTCTGCTTAATTAC+TGG + chr2.1:28437227-28437246 MS.gene039174:CDS 30.0%
AATAGATCTATGAAGTTGCA+GGG + chr2.1:28439311-28439330 MS.gene039174:intron 30.0%
ATACACAAAAAACCTAAGCT+CGG + chr2.1:28438464-28438483 MS.gene039174:intron 30.0%
ATCCCTGTTCTTATTCATAA+TGG + chr2.1:28437376-28437395 MS.gene039174:CDS 30.0%
CAAAGGCTTAAATATGGTAT+TGG + chr2.1:28437724-28437743 MS.gene039174:intron 30.0%
CCTCTAAACCAATTGTATAA+TGG - chr2.1:28439478-28439497 None:intergenic 30.0%
GGCTGATTATATTGATAAGA+AGG + chr2.1:28437510-28437529 MS.gene039174:CDS 30.0%
GGTTCATTTGTAACAACAAA+GGG - chr2.1:28437342-28437361 None:intergenic 30.0%
TAAGATCCATGATTTGATTG+TGG + chr2.1:28439609-28439628 MS.gene039174:CDS 30.0%
TCTAAATACACAAAAGTTCC+CGG + chr2.1:28438381-28438400 MS.gene039174:intron 30.0%
TGTATTTATAAACCGAGCTT+AGG - chr2.1:28438479-28438498 None:intergenic 30.0%
TTATTATATAACTGGGGTCA+GGG + chr2.1:28439061-28439080 MS.gene039174:intron 30.0%
TTTATTATATAACTGGGGTC+AGG + chr2.1:28439060-28439079 MS.gene039174:intron 30.0%
! CCATTATACAATTGGTTTAG+AGG + chr2.1:28439475-28439494 MS.gene039174:CDS 30.0%
! TTTTTGATCCCTGCAAATAT+AGG + chr2.1:28437860-28437879 MS.gene039174:intron 30.0%
!! AAAACTGGTCCATTTTTGTA+GGG + chr2.1:28439123-28439142 MS.gene039174:intron 30.0%
!!! CCCTGCAAAATTTTTGTTTT+TGG - chr2.1:28439094-28439113 None:intergenic 30.0%
!!! TTCTTGCTCTTCTTCTTTTT+TGG - chr2.1:28439347-28439366 None:intergenic 30.0%
!!! TTGGGAGACAAGAATTTTTT+TGG + chr2.1:28439415-28439434 MS.gene039174:CDS 30.0%
AAAATAGCCCTAGCTGAAAA+GGG + chr2.1:28437274-28437293 MS.gene039174:CDS 35.0%
GGGTTCATTTGTAACAACAA+AGG - chr2.1:28437343-28437362 None:intergenic 35.0%
TAAGGATCAGAAGGTAACAA+AGG - chr2.1:28437466-28437485 None:intergenic 35.0%
TTCAATCTGCATGCATCTAT+TGG - chr2.1:28439560-28439579 None:intergenic 35.0%
TTTGGTCCCTACAAAAATTC+TGG + chr2.1:28437926-28437945 MS.gene039174:intron 35.0%
! AAAACTGGCCTATATTTGCA+GGG - chr2.1:28437871-28437890 None:intergenic 35.0%
! ACAGGGATTTTCTTATGAAC+AGG - chr2.1:28437364-28437383 None:intergenic 35.0%
! CAGGGATTTTCTTATGAACA+GGG - chr2.1:28437363-28437382 None:intergenic 35.0%
! CTACAAAAATGGACCAGTTT+TGG - chr2.1:28439124-28439143 None:intergenic 35.0%
!! ATTTTGAGTCTCATGCCAAA+AGG - chr2.1:28437259-28437278 None:intergenic 35.0%
!! CAAAACTGGTCCATTTTTGT+AGG + chr2.1:28439122-28439141 MS.gene039174:intron 35.0%
!!! GGAGACAAGAATTTTTTTGG+AGG + chr2.1:28439418-28439437 MS.gene039174:CDS 35.0%
!!! GTTTTTGGTCCCTACAAAAA+TGG - chr2.1:28437942-28437923 None:intergenic 35.0%
!!! GTTTTTGGTCCTTGCAAAAT+AGG + chr2.1:28437799-28437818 MS.gene039174:intron 35.0%
AAACTCTTGGAGCAAGAGTT+GGG + chr2.1:28439397-28439416 MS.gene039174:CDS 40.0%
ATGAAGTTGCAGGGAACAAT+TGG + chr2.1:28439320-28439339 MS.gene039174:CDS 40.0%
CAAAATAGCCCTAGCTGAAA+AGG + chr2.1:28437273-28437292 MS.gene039174:CDS 40.0%
CATGGATCTTATCCTGATCA+GGG - chr2.1:28439600-28439619 None:intergenic 40.0%
GCTTTCTGATAAGGATCAGA+AGG - chr2.1:28437475-28437494 None:intergenic 40.0%
TCATGGATCTTATCCTGATC+AGG - chr2.1:28439601-28439620 None:intergenic 40.0%
TGAGCTCCACAATCAAATCA+TGG - chr2.1:28439618-28439637 None:intergenic 40.0%
! AATTACTGGCCAAGTCCTTT+TGG + chr2.1:28437241-28437260 MS.gene039174:CDS 40.0%
! AGAAAGCACAAGCTAGATTC+TGG + chr2.1:28437488-28437507 MS.gene039174:CDS 40.0%
! CAAAACTGGCCTATATTTGC+AGG - chr2.1:28437872-28437891 None:intergenic 40.0%
! CAATTGGTTTAGAGGCTATG+AGG + chr2.1:28439483-28439502 MS.gene039174:CDS 40.0%
! CTAGCTTGTGCTTTCTGATA+AGG - chr2.1:28437484-28437503 None:intergenic 40.0%
! CTGCAAATATAGGCCAGTTT+TGG + chr2.1:28437870-28437889 MS.gene039174:intron 40.0%
! CTTGATACCCTTTTCAGCTA+GGG - chr2.1:28437284-28437303 None:intergenic 40.0%
! CTTGCAAAATAGGCCAGTTT+TGG + chr2.1:28437809-28437828 MS.gene039174:intron 40.0%
! GAAAGCACAAGCTAGATTCT+GGG + chr2.1:28437489-28437508 MS.gene039174:CDS 40.0%
!! CAAAACTGGCCTATTTTGCA+AGG - chr2.1:28439202-28439183 None:intergenic 40.0%
!! CAAAACTGGCCTATTTTGCA+GGG + chr2.1:28439183-28439202 MS.gene039174:intron 40.0%
!! CAAAACTGGCCTATTTTGCA+GGG - chr2.1:28439202-28439183 None:intergenic 40.0%
!!! TTTTTTGGAGGAGACAAGCT+TGG + chr2.1:28439430-28439449 MS.gene039174:CDS 40.0%
ACTCTTGCTCCAAGAGTTTG+AGG - chr2.1:28439396-28439415 None:intergenic 45.0%
AGAGCAAGAAGCTGCAAAGA+AGG + chr2.1:28439357-28439376 MS.gene039174:CDS 45.0%
AGTCTCATGCCAAAAGGACT+TGG - chr2.1:28437253-28437272 None:intergenic 45.0%
CAAACTCTTGGAGCAAGAGT+TGG + chr2.1:28439396-28439415 MS.gene039174:CDS 45.0%
CATAGAAGCCCTCAAACTCT+TGG + chr2.1:28439384-28439403 MS.gene039174:CDS 45.0%
CTCTTGCTCCAAGAGTTTGA+GGG - chr2.1:28439395-28439414 None:intergenic 45.0%
GCAGGGACTAAAACCAAAAC+TGG + chr2.1:28439108-28439127 MS.gene039174:intron 45.0%
GCAGGGACTAAAACCAAAAC+TGG - chr2.1:28439127-28439108 None:intergenic 45.0%
GTCTACACTGATCTTGCCAA+AGG - chr2.1:28439509-28439528 None:intergenic 45.0%
GTGGAGCTCAAGAAGAAGTA+TGG + chr2.1:28439628-28439647 MS.gene039174:CDS 45.0%
TGGCAAGATCAGTGTAGACA+AGG + chr2.1:28439510-28439529 MS.gene039174:CDS 45.0%
TTTAGAGGCTATGAGGCCTT+TGG + chr2.1:28439490-28439509 MS.gene039174:CDS 45.0%
! CCTGCAAAATAGGCCAGTTT+TGG + chr2.1:28437749-28437768 MS.gene039174:intron 45.0%
! CCTGCAAAATAGGCCAGTTT+TGG - chr2.1:28437768-28437749 None:intergenic 45.0%
! GCTTGATACCCTTTTCAGCT+AGG - chr2.1:28437285-28437304 None:intergenic 45.0%
!! CCAAAACTGGCCTATTTTGC+AGG + chr2.1:28439182-28439201 MS.gene039174:intron 45.0%
!! CCAAAACTGGCCTATTTTGC+AGG - chr2.1:28439201-28439182 None:intergenic 45.0%
!! GGTATTGGTCCCTGCAAAAT+AGG + chr2.1:28437739-28437758 MS.gene039174:intron 45.0%
!! GGTATTGGTCCCTGCAAAAT+AGG - chr2.1:28437758-28437739 None:intergenic 45.0%
!! AAAAAAAAATTAAAAAATTC+TGG - chr2.1:28439033-28439052 None:intergenic 5.0%
!! AAAAAAAATTAAAAAATTCT+GGG - chr2.1:28439032-28439051 None:intergenic 5.0%
!! AAAAAAATATTAAAAAAATC+TGG - chr2.1:28438916-28438935 None:intergenic 5.0%
!! AAAAAAATATTAAAAAATTC+TGG + chr2.1:28437975-28437994 MS.gene039174:intron 5.0%
!! AAAAAATTAGAAAAAAAATA+AGG - chr2.1:28438816-28438835 None:intergenic 5.0%
!!! ATATTTTTTTATTATATAAC+TGG + chr2.1:28439053-28439072 MS.gene039174:intron 5.0%
!!! ATTTTTTTTTATATAGTTTT+TGG + chr2.1:28437908-28437927 MS.gene039174:intron 5.0%
!!! TATTTTTTTATTATATAACT+GGG + chr2.1:28439054-28439073 MS.gene039174:intron 5.0%
AGTGCCCTAAGTTCAGTGCT+TGG + chr2.1:28439533-28439552 MS.gene039174:CDS 50.0%
ATCCTGATCAGGGAGTGACT+TGG - chr2.1:28439590-28439609 None:intergenic 50.0%
GTGCCCTAAGTTCAGTGCTT+GGG + chr2.1:28439534-28439553 MS.gene039174:CDS 50.0%
TTCCAAGTCACTCCCTGATC+AGG + chr2.1:28439585-28439604 MS.gene039174:CDS 50.0%
!! TGGCCCAAGCACTGAACTTA+GGG - chr2.1:28439540-28439559 None:intergenic 50.0%
!! TTGGCCCAAGCACTGAACTT+AGG - chr2.1:28439541-28439560 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 28437217 28439660 28437217 ID=MS.gene039174
chr2.1 mRNA 28437217 28439660 28437217 ID=MS.gene039174.t1;Parent=MS.gene039174
chr2.1 exon 28437217 28437531 28437217 ID=MS.gene039174.t1.exon1;Parent=MS.gene039174.t1
chr2.1 CDS 28437217 28437531 28437217 ID=cds.MS.gene039174.t1;Parent=MS.gene039174.t1
chr2.1 exon 28439316 28439660 28439316 ID=MS.gene039174.t1.exon2;Parent=MS.gene039174.t1
chr2.1 CDS 28439316 28439660 28439316 ID=cds.MS.gene039174.t1;Parent=MS.gene039174.t1
Gene Sequence

>MS.gene039174

ATGGCTGATGAAGTGATTCTGCTTAATTACTGGCCAAGTCCTTTTGGCATGAGACTCAAAATAGCCCTAGCTGAAAAGGGTATCAAGCATGAGTACAGAGAAGAAGACTTAAGCAACAAAAGCCCTTTGTTGTTACAAATGAACCCTGTTCATAAGAAAATCCCTGTTCTTATTCATAATGGAAAACCAATTTGTGAATCTTTAATTGCTGTTCAGTATATTGATGAAGTTTGGAATGATAAATCTCCTTTGTTACCTTCTGATCCTTATCAGAAAGCACAAGCTAGATTCTGGGCTGATTATATTGATAAGAAGGTAAGATGTATTTCAAAACAAGCTTAATTTTTGAATCAACATTTTGTGTGCAATAATTTCTGAAAAGTTTAGACATTGGCTTTCTGTTCAACTTAATATGATGATTTATTGTGTATCTAAGTTAAGAATTTTTGAGATAATTCATATTGGGAGCACCCATTTGATATTTTTAGCATGCATAATCATTATTAGCAAAGGCTTAAATATGGTATTGGTCCCTGCAAAATAGGCCAGTTTTGGTTTTAGTCCCTGCAATTTTTTTTTATAGTTTTTGGTCCTTGCAAAATAGGCCAGTTTTGGTTTTAGTCCCTGCAAATTTTTTTTATAGTTTTTGATCCCTGCAAATATAGGCCAGTTTTGGTTTTAGTCCCTGCAAATTTTTTTTTATATAGTTTTTGGTCCCTACAAAAATTCTGGAAAAATGAAAAATTAATAAAAAAATAAAAAAAATATTAAAAAATTCTGGAAAATTGAAAAATTAATAAAAAAATATTAAAAAATTCTGGAAAAATGAAAAATTAATTAAAAAATTGAAAAAAATATTAAAAAATTCTGGAAAAACGAAAAATTAATAAAAAATTGAAAAAAATATTTAAAAATTCTAAAAAATTAAAAATTCTGGAAAAATTAATAAAAATAAAAAACGATTAAAAAAATAAAATAATCTAATAAAATGAAAAATAATGCTAAATTCTGAAAAAAAAATAAAAAAGATTAAAAAATTCTGAAAAAATAAAATTTTGAAATTTTCTAAAAAAAATTAAAATTCTAAAAAAATTAAATTTTCGAAATTTTAATGAAAAATTTTAGTTCAAATTTTCAAAATTTTAATAAAAATTCTGGAAAAATTCTAAATACACAAAAGTTCCCGGTTTATAAATACACAAAAATTCTTGGAAAAAATACTAATGAAAATTTCGAAATTTCGAAAAATACACAAAAAACCTAAGCTCGGTTTATAAATACACATATTTTTTCCCTTATTCTCGGTTTATAAATACGCAAAATTCCCGGAAATTTTTTTGGAAATAAATACACTGAAAAAACTTAAGCTCGACATATATACTAATGAAATTTTCCAGAATTTTTATTAAAATTTCCAAAATTTCATTTTTCCAGAATTTTAATTTTTTTCAGAAAATTTCGAAATTTTATTTTTTCAGAATTTTTTAATCTTTTTTATTTTTTCTGAATTTATCATTATTTTTCATTTTATTAGATTATTTTATTTTTTAATCATTTTTTATTTTTATTAATTTTTCCAGAATTTTTAAATTTTTTCCTTATTTTTTTTCTAATTTTTTTTCATTTTTATTAATTTTTCAATTTTCCAAAATTTTTTAATATTTTTTTTCAATTTTTTTATTAGTTTTTCATTTTTCCAGATTTTTTTAATATTTTTTTAATTTTTTATTAACTTTTCAATTTTTCAGAATTTTTTAATATTTTTTTCAATTTTTTATTAATTTTTCATTTTTTCCAGAATTTTTTTATTTTCCCAGAATTTTTTAATTTTTTTTTATATTTTTTTATTATATAACTGGGGTCAGGGACTATTTCCAAAAACAAAAATTTTGCAGGGACTAAAACCAAAACTGGTCCATTTTTGTAGGGACCAAAAACTATAAAAAAAATTTGCAGGGACTAAAACCAAAACTGGCCTATTTTGCAGGGACCAATACCATATTTAAGCCTATTAGCAAATAACATGATACTTTTTGTTTTTCATGTTCTTGCATGTTATGATCTAATAAATCAGTGTGTGTGAATTCTCTGTCAAATAGATCTATGAAGTTGCAGGGAACAATTGGACCAAAAAAGAAGAAGAGCAAGAAGCTGCAAAGAAGGAATTCATAGAAGCCCTCAAACTCTTGGAGCAAGAGTTGGGAGACAAGAATTTTTTTGGAGGAGACAAGCTTGGTTTTGTTGATGTAGCATTTATTCCATTATACAATTGGTTTAGAGGCTATGAGGCCTTTGGCAAGATCAGTGTAGACAAGGAGTGCCCTAAGTTCAGTGCTTGGGCCAATAGATGCATGCAGATTGAAAGTGTTTCCAAGTCACTCCCTGATCAGGATAAGATCCATGATTTGATTGTGGAGCTCAAGAAGAAGTATGGCTTTGAGTAG
Protein sequence

>MS.gene039174.t1

MADEVILLNYWPSPFGMRLKIALAEKGIKHEYREEDLSNKSPLLLQMNPVHKKIPVLIHNGKPICESLIAVQYIDEVWNDKSPLLPSDPYQKAQARFWADYIDKKIYEVAGNNWTKKEEEQEAAKKEFIEALKLLEQELGDKNFFGGDKLGFVDVAFIPLYNWFRGYEAFGKISVDKECPKFSAWANRCMQIESVSKSLPDQDKIHDLIVELKKKYGFE